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Villanueva
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N. Villanueva
TABLE OF CONTENTS
Parastrongylus cantonensis 62
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• Parasite that does not live in its usual Water Borne • Drinking contaminated water
host • Giardia, Cryptosporidium
Spurious • Free-living organism that passes Vertical • Congenital transmission
Parasite through the GI tract without infecting Transmission • Toxoplasma gondii
the host Transmammary • Drinking of breast milk
Temporary • Transient parasites • Ancylostoma, Strongyloides
Permanent • Remains on host for its entire life Skin Penetration • Exposure of skin to soil or water
Parasites According to Egg Laying Capacity • Hookworms, Strongyloides,
Oviparous • Lays immature eggs (eggs not yet Schistosoma
embryonated, egg has no larva yet) Inhalation • Of airborne eggs
• Ex: Ascaris, Trichuris • Enterobius
Ovoviviparous • Lays mature eggs (embryonated, Intimate Contact • Sexual contact
larva present) • Trichomonas vaginalis
• Ex: Schistosoma, Clonorchis
Larviparous • Larva-laying
• Ex: Trichinella LIFE CYCLES
Parasites According to Sexes
Monoecious • Also known as Hermaphrodites • Life cycles: how the parasite develops
• Both testes and ovaries found in one • Can be direct or indirect
parasite • Direct: no intermediate host, only consists of a parasite and
• Ex: Flukes and Tapeworms a final host
Dioecious • Presence of separate sexes • Indirect: has an intermediate host
• Female and male parasite o Migration of larval stages present in some parasites
• Ex: Nematodes (except o Ex: Plasmodium
Strongyloides) • Life cycle more complicated = lesser chances for parasite to
Parthenogenetic • Females capable of self-fertilization survive
• Ex: Strongyloides stercoralis
EXPOSURE AND INFECTION
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AMEBA, FLAGELLATES, & CILIATES
Life Cycle
Ingestion of Cyst • Cyst goes to stomach
• Excystation takes place
• pH should be alkaline or neutral (acidic
pH does not favor formation of
trophozoites)
Multiplication of • takes place in large intestine
Trophozoites • multiplication through binary fission
• usually produces 4 trophozoites from 1
cyst (but not for all organisms!)
Trophozoites and • whether trophozoites or cysts appear
Cysts go to the depends on the type of stool (if formed
stool or watery)
Entamoeba histolytica
•MOT: ingestion of infective cyst
•Habitat: large intestine
•Only pathogenic amoeba
•Subphylum Sarcodina, superclass Rhizopoda, class Lobosea, order Amoebida, family Entamoebidae
•Cyst is resistant to gastric acidity and desiccation, can survive in a moist environment for several weeks
•Trophozoites multiply by binary fission
•Entamoeba species: spherical nucleus, distinct nuclear membrane lined with chromatin granules, small karyosome near center of
nucleus
Trophozoite Cyst
Nucleus 1 nucleus (vesicular appearance) 4 nuclei (ideally)
Karyosome Centrally located karyosome Small, centrally located karyosome
Peripheral chromatin Fine, evenly distributed Fine, evenly distributed
Appearance Clean-looking cytoplasm Thin wall, hyaline appearance, highly refractile
Additional structures • Finger-like appearance of pseudopodia Chromatoidal bar
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COMMENSAL AMEBAE
Entamoeba coli
• Transmitted via ingestion of infective cyst
• More common than other human amebae
Trophozoite Cyst
Nucleus 1 nucleus 8 nuclei
Karyosome Eccentric Eccentric
Peripheral chromatin Coarse, rough Coarse, rough
Appearance Dirty-looking Larger than E. histolytica,
cytoplasm (contains thick cystic wall
bacteria, debris,
yeast)
Entamoeba hartmanni
• Small race of E. histolytica
Trophozoite Cyst
Nucleus 1 nucleus 1-2 nuclei only (mature cyst can have 1-4 nuclei)
Karyosome Centrally located Centrally located
Peripheral chromatin Fine, evenly distributed Fine, evenly distributed
Additional structures Pseudopod Diffuse glycogen
(similar to E. vacuole/mass (not
histolytica) seen in permanent
stain)
Entamoeba polecki
• Ameba of pigs and monkeys
• Most common parasite in Papua New Guinea
• May resemble other Entamoeba species
• Zoonotic infection: can be passed from animals to humans
Trophozoite Cyst
Nucleus 1 nucleus 1 nucleus
Karyosome Centrally located Large, centrally located
Appearance Almost the same appearance as E. histolytica Almost the same appearance as E. histolytica
Additional structures Similar to E. Chromatoidal bar
histolytica • Angular/pointed
appearance
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Endolimax nana
• Smallest intestinal amebae (as small as RBC, 6-8 um)
• Commensal
• Endolimax: vesicular nucleus with a relatively large, irregularly shaped karyosome
Trophozoite Cyst
Nucleus 1 nucleus 4 nuclei
Karyosome Large, irregular Large, prominent, blot-like
Peripheral chromatin None None (this is only found in Entamoebas!)
Appearance Ingested bacteria, Oval, cross-eyed
blunt and hyaline
pseudopodia, food
vacuoles are also
present (which
may contain
bacteria)
Iodamoeba butschlii
• ameba of swine (pigs)
• large, chromatin-rich karyosome surrounded by a layer of achromatic globules and anchored to the nuclear membrane by
achromatic fibrils
Trophozoite Cyst
Nucleus 1 nucleus 1 nucleus
Karyosome Large, eccentric Large, eccentric
Peripheral chromatin None None
Appearance “Basket of Oval, also has
Flowers” basket of flowers
appearance appearance
(due to
achromatic
granules),
triangular
shaped
Additional structures Glycogen vacuole (Iodine used to visualize this) Glycogen vacuole (Iodine used to visualize this)
Motility Sluggish, non-progressive Nonmotile
Entamoeba gingivalis
• NO CYST STAGE
• Ameba of oral cavity (gum line)
• Also found in tartar, gingival pockets of teeth, and tonsillar crypts (of unhealthy mouths, but may
also be in healthy mouths)
• May also be seen in the genital tract
• Scavengers, eat debris
• Can also ingest RBCs (but it’s rare)
• Transmitted via direct-contact (kissing, sharing of personal items)
• First amoeba in man
• Can be seen in sputum sample (can go to the lungs)
• Also found in AIDS patients
• Found even in healthy people
• Non-pathogenic, but can be seen in patients with pyorrhea alveolaris (gum infections)
Trophozoite
Nucleus 1 nucleus
Peripheral chromatin Fine, evenly distributed
Additional structures • Capable of ingesting WBCs
• Numerous, blunt pseudopodia
• Numerous food vacuoles that contain cellular debris (mostly leukocytes from the ingested WBCs)
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Entamoeba histolytica
Disease manifestation Signs and Symptoms
Asymptomatic Carrier State Excrete cysts
(90% of cases)
Intestinal Disease (10% of • Incubation period: 1-4 weeks
cases) • Bloody diarrhea, dysentery (majority of cases), abdominal pain, flatulence, weight loss,
chronic fatigue
• Release of enzymes to lyse mucosal lining
• Formation of flask-shaped ulcers by the trophozoites
• Excess mucus in stool
• Tenesmus: cramping rectal pain
• 10 bowel movements per day
• Clinical Forms:
o Fulminating Colitis (inflammation of colon)
▪ Can lead to perforation and secondary bacterial peritonitis (most serious complications)
o Amebic Appendicitis
o Ameboma (granulomas, chronic inflammations, can be mistaken as carcinomas or cancer)
Extra-intestinal Disease (usually • Ectopic form of amebiasis
affects the upper lobe of the • Amebic Liver Abscess (ALA): liver aspirate (like anchovy sauce) where you can find
liver because blood vessels are trophozoites
connected to the small o Can lead to rupture into the pericardium, rupture into the pleura, super infection, and
intestine) intraperitoneal rupture
• Cardinal signs: fever and right upper quadrant pain
• Tender liver (tender: painful when you touch or palpate)
• Hepatomegaly (abnormal enlargement of liver)
• Cutaneous Amebiasis (amebiasis cutis): rare, infection of skin and soft tissue
• Skin rupture
• Affects inguinal areas
• Can be transmitted sexually
• *amebiasis is characterized by low amount of WBCs in stool
• Can cause lung abscess (found in sputum) and brain abscess
• Secondary amebic meningoencephalitis (occurs in 1-2%)
• Renal involvement is rare
• Genital involvement
o Caused by fistulae from ALA and colitis or primary infection through sexual transmission
Amebiasis IS DIFFERENT from bacterial dysentery
• In amebiasis, there is mucus and blood in the stool
• There is no granulocytosis and no high fever
• There is also a fishy smell of the stool
Laboratory Diagnosis for Commensal Amebae
• Stool examination
• FECT and iodine stain useful to differentiate the species
• E. gingivalis: swab between gums and teeth (examined for trophozoites)
• DFS
• Concentration techniques (FECT and zinc sulfate flotation) useful for recovering cysts
*no treatment necessary for commensal amebae (they do not cause disease)
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• Found inhabiting lakes, pools, tap water, air conditioning units, and heating units
• Parasites are facultative (with a free-living and parasitic phase)
Life Cycle
Person swims in • Parasite enters via olfactory region
contaminated • Parasite can also enter through the skin
water • Parasite cannot survive in salt water
• Infective stage: trophozoite
Trophozoite goes • Targets the CNS
straight to the brain • Brain tissue or CSF can be examined
Naegleria fowleri
• Belongs to family Vahlkampfiidae
• Free-living ameboflagellate (has an ameba and flagellate form)
• Only Naegleria species that can infect humans
• Thermophilic: thrive best in hot springs and other warm aquatic environments
• Trophozoites replicate by promitosis
• Cyst found only in the environment
• Enters the body through the olfactory epithelium, respiratory tract, and the skin and sinuses
• Targets the brain tissue (trophozoite goes straight to the brain)
• Cyst: spherical and single-walled
• Trophozoite: 1 nucleus, large and dense karyosome, cytoplasm is granular and contains many vacuoles
o Ameboid form: Limax-form (slug-like)
o Ameboflagellate: 2 anterior flagella
• Trophozoites also characterized by blunt, lobose pseudopodia and directional motility
Disease Manifestation • Primary Amebic Meningoencephalitis (PAM)
and Pathology o Inflammation of meninges in the brain
o Can affect healthy people, fast progression
o Very fatal
o When you swim in contaminated pools, lakes, and rivers
o Signs and symptoms: headache, fever, nausea, vomiting, nuchal rigidity, rhinitis, lethargy, olfactory
problems, mental status changes, mental confusion, coma
o Incubation period: 2-3 days or 1-2 weeks
o Patients usually dead after 1 week
o Brain has hemorrhaging (has lots of WBCs, especially neutrophils)
o Usually diagnosed post-mortem
o Few cases in the PH, usually in US
• Pathogenic determinant (virulence factor)
o Presence of amebostomes (food cups)
o Used to attach to the brain
o Releases enzymes (phospholipases) to destroy brain tissue
o Other pathogenic determinants include (produces a cytopathic effect on host tissues):
▪ Secretion of lytic enzymes
▪ Membrane pore-forming proteins
▪ Induction of apoptosis
▪ Direct feeding of the ameba
Laboratory Diagnosis • Wet mount examination of CSF (look for trophozoite)
• Smears stained with Wright’s or Giemsa
• Biopsy of tissue
• CSF Analysis
o Nonspecific for N. fowleri
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Life Cycle
Parasite enters through Goes to brain and affects CNS
the nose
Parasite enters through Causes blindness
the eyes
Parasite enters through Causes lesions on the skin (especially in AIDS
ulcerations in the skin patients)
Reproduce in the body Through mitosis
Acanthamoeba spp.
• Family Acanthamoebidae
• Acanthamoeba castellani (most common); A. culbertsoni; A. hutchetti; A. polyphaga; A. rhysoides
• Free-living ameba
• Aquatic organism
• Found in a myriad of natural and artificial environments
• Can survive even in contact lens solutions
• Entry can occur through the eyes, nasal passages to the lower respiratory tract, or ulcerated or broken skin
• Possible reservoir hosts for medically important bacteria such as Legionella spp., mycobacteria and gram-negative bacilli such
as E. coli
• Both trophozoite and cyst are its infective stages
• Trophozoites reproduce by binary fission
• Trophozoites
o Eats gram negative bacteria, blue-green algae, or yeasts
o Can adapt to feed on corneal epithelial cells and neurologic tissue
▪ through phagocytosis and secretion of lytic enzymes
• Trophozoite transforms to cyst when environmental conditions are unfavorable
Trophozoite Cyst
Nucleus Single large nucleus Single large nucleus
Karyosome Centrally located, densely staining Large karyosome
Additional structures • characteristic “thorn-like” appendages (acanthapodia) • Double-walled cyst
o “Acantha”- spring o Outer wall: wrinkled
o “Spring projections of the pseudopod” o Inner wall: polygonal
o For locomotion
o Evident on phase-contrast microscope
• Contractile vacuoles
• Large endosome
• Finely granulated
cytoplasm
• Eats gram negative
bacteria
• Can eat the host’s
tissues
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Balamuthia mandrillaris
• Family Leptomyxidae
• New species causing amebic meningoencephalitis
• Also causes Granulomatous Amebic Encephalitis (GAE)
• Cysts have a characteristic wavy appearance
• Trophozoites are branching
• Almost the same appearance with Acanthamoeba
• Both cysts and trophozoites can be seen in the brain (when infected)
Other free-living ameba that causes amebic encephalitis
• Sappinia diploidea
• Hartmanella vermiformis – considered now as opportunistic
INTESTINAL FLAGELLATES
• All inhabit the large intestine, except Giardia lamblia (small intestine), Trichomonas vaginalis (urogenital), Trichomonas tenax (mouth)
• All undergo encystation, except Trichomonas species and Dientamoeba fragilis
• All are commensals except Giardia lamblia, Dientamoeba fragilis, Trichomonas vaginalis
• Flagella is attached to the blepharoplast found on the body of the parasite
• All undergo asexual reproduction through binary fission
Life Cycle
Ingestion of cyst • Released in the small intestine
• Excystation takes place
Reproduction Reproduce through binary fission
takes place in the (longitudinal)
small intestine
Parasite passed in • Either cyst or trophozoite
the stool • Depends on type of stool
Giardia lamblia
• Also known as G. duodenalis and G. intestinalis
• Mode of transmission: ingestion of infective cysts (from fecally contaminated water or food)
• Zoonotic
• Habitat: small intestine (duodenum, jejunum, and upper ileum), only one in the small intestine, the rest of the intestinal
flagellates are located in the large intestine
• Low infective dose (only need to ingest around 8-10 cysts to be infected, reason for outbreaks of diarrhea)
• Beavers: reservoir hosts
• Reproduce by binary fission, longitudinal
• Prefers alkaline pH (7.8-8.2), the more alkaline, the more it attaches
Trophozoite Cyst
Nuclei 2 nuclei (ovoidal) 4 nuclei
Appearance Pear/pyriform shape, old man’s face with Refractile/clear cyst wall (hyaline), oval shaped
eyeglasses
Additional structures • Axostyle • Median/parabasal bodies (2)
▪ For support ▪ Energy structures
• 1 pair anterior flagella • Axoneme (multiple axostyles)
• 2 pairs lateral flagella • Deeply stained curved fibrils
• 1 pair conal/posterior flagella
• 2 Ventral sucking discs (virulence factor)
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• Serology
• Molecular methods
• Biopsy
o Tissue from intestine
o Check for flattening of villi
Life Cycle
Ingestion of • Goes to the large intestine
trophozoites
Reproduction takes • Replicates by binary fission
place • Trophozoites will be located in the lumen of
the colon
Dientamoeba fragilis
Trophozoite
Nucleus 2 nuclei (hence Dientamoeba)
Karyosome Rosette/rose-like
Appearance • sometimes not detected/seen
▪ fragilis – fragile
▪ its easily destroyed
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Treatment • Iodoquinol
• Other drugs: tetracycline and metronidazole
Life Cycle
Ingestion of cysts Parasite goes to the colon/large intestine
and develops and reproduces
Parasite is passed Cysts usually contaminates food, water,
in the feces hands of people, and other fomites
Chilomastix mesnili
Trophozoite Cyst
Nucleus 1 nucleus, with prominent karyosome 1 nucleus
Appearance Pyriform, pear-shaped, curved posture, twisted jaw • American lemon appearance
appearance • Nipple-shaped cyst
• 7-10 um in size
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Enteromonas hominis
• Commensal
• Almost the same life cycle as Chilomastix
• Mode of transmission: ingestion of cysts
o Contamination of water, food, or hands/fomites with infective cysts
Trophozoite Cyst
Nucleus 1 nucleus 2 or 4 nuclei (located at ends of the cyst)
Appearance Oval-shaped Oval-shaped
• 3 anterior
flagella
• 1 posterior
flagella
Retortamonas intestinalis
• Commensal
• Mode of transmission: ingestion of cysts
• Same life cycle as E. hominis
o Contamination of water, food, or hands/fomites with infective cysts
Trophozoite Cyst
Nucleus 1 nucleus 1 nucleus
Appearance • 1 anterior and 1 posterior • Pear-shaped or slightly
flagella lemon-shaped
• Cytostomal fibril
o Bird’s beak
• Cytostome: cleft-like
appearance
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UROGENITAL FLAGELLATES
Trichomonas vaginalis
Trophozoite
Nucleus 1 nucleus
Appearance Pyriform, Pear-shaped
Additional structures • 4 anterior flagella
• 1 flagella embedded in the undulating membrane
• Axostyle
• Cytostome
• Undulating membrane
o Found on the lateral portion
o Wave-like structure
o For motility
o Length is crucial for identification
o ½ of body length
o Attached to body of parasite via costa
▪ Rib-like structure
▪ Distinct for this parasite
• Siderophil granules
o Also known as paraxostylar granules
o Iron-rich
o Near axostyle
o No distinct function (only for identification)
• Vacuole with bacteria
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Laboratory Diagnosis • Wet Mounts of vaginal and urethral discharge (can also use urine samples)
o To check motility
o Low sensitivity
• Stained smears (Giemsa or Pap’s)
• Culture: Diamond modified medium, Feinberg Whittington, Cysteine Peptone Liver
Maltose, Simplified Trypticase Serum Semen Culture
o Gold standard, takes 2-5 days
o Best results seen with combination of urethral swabs and urine sediment
• Antigen detection
• Serology
• Molecular methods
• PCR (detects more cases with men than women)
• InPouch™ TV: allows specimen to be inoculated into a sealed pouch with culture media
Pentatrichomonas hominis
• Commensal
• Formerly known as Trichomonas hominis
• Penta: has 5 flagella
• Habitat: colon
• Trophozoites found in contaminated food, water, or hands/fomites
Trophozoite
Nucleus 1 nucleus
Additional structures • 4 anterior flagella
• 1 posterior flagella
• Conical cytostome
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• Axostyle
• No peripheral chromatin
• Undulating membrane
o Full body length
Trichomonas tenax
• Commensal
• Habitat: mouth (tartar of teeth, cavities of carious teeth, necrotic mucosal cells in gingival margins)
• Mode of transmission: direct contact (kissing) or use of contaminated glass or dishes (sharing of utensils)
• Generally harmless
o Though there are reports of respiratory infections and thoracic abscesses in cancer and other
immunocompromised patients or in patients with other lung diseases
o Pulmonary trichomoniasis reported among those with underlying chronic pulmonary disease
o Parasite probably unable to cause disease on its own
o Presence of bacteria most probably allows it to proliferate profusely
• Smallest among the species
• Resistant to changes in temperature
• Will survive for several hours in drinking water
• Diagnosis through swabbing tartar between teeth, gingival margin, or tonsillar crypts
• Treatment: metronidazole
Trophozoite
Nucleus 1 nucleus
Appearance Pyriform
Additional structures • 4 anterior flagella
• 1 posterior flagella
• Axostyle
• Cytostome
• Undulating membrane
o 2/3 body length
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CILIATES
Balantidium coli
• Largest protozoan infecting man
• Hosts: man (pigs usually reservoir hosts, zoonotic)
• Mode of transmission: ingestion of cysts
• Habitat: colon (cecum)
• Risk factors: close contact with pigs (in pig feces)
• Cysts found in fecally contaminated food or water
• Only ciliate known to cause human disease
Trophozoite Cyst
Nucleus 2 nuclei 2 nuclei (micronucleus and macronucleus), unlike in
• Micronucleus: For sexual reproduction amebae, encystation does not result in an increase of
o lies in concavity of macronucleus nuclei
• Macronucleus: Kidney-shaped
o for asexual reproduction and
vegetative function
• Mucocysts
o Extrusive organelles
o Located beneath cell membrane
Appearance Ellipsoid, tapered in anterior portion Spherical/Ellipsoidal (oval)
Additional • Cytostome (can be found by observing the • Cyst wall
structures tapered portion, since the anterior portion is o Double-walled
tapered) o Refractile (shiny)
o Oral apparatus • Cilia is enclosed within the cyst wall
o Through which it acquires food
• Cytopyge
(anus)
o Through
which it
excretes
waste
• Contractile
vacuoles: for
osmoregulation
• Food vacuoles
• Cilia
Motility Thrown-ball motility Nonmotile
Disease • Incubation period: 4-5 days
Manifestation • Causes Balantidiasis or Balantidial Dysentery
o Bloody Diarrhea
o Flask-shaped ulcers (wider and rounded)
o Extraintestinal spread may occur
• Virulence factor: hyaluronidase
o Lytic enzyme that causes ulceration
• Presence of Salmonella has been shown to aggravate Balantidiasis (by invading ulcers caused by the
protozoan)
• Three clinical manifestations
o Asymptomatic
▪ Do not present diarrhea
▪ Serve as parasite reservoir
o Acute Cases (Fulminant Balantidiasis)
▪ Diarrhea with bloody and mucoid stools
▪ Often associated with immunocompromised and malnourished states
o Chronic Cases
▪ Diarrhea may alternate with constipation
▪ Accompanied with abdominal pain, cramping, anemia, and cachexia
• Can spread to extraintestinal sites
o Mesenteric nodes
o Appendix
o Liver
o Genitourinary sites
o Pleura
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o Lungs
• Complications include intestinal perforation and acute appendicitis
Laboratory • Direct examination or concentration techniques
Diagnosis o Sedimentation or floatation
o Feces with trophozoites and cysts
• Biopsy specimens (from lesions obtained through sigmoidoscopy)
• Bronchoalveolar washings (in case of pulmonary infection)
Epidemiology • Prevalence (0.02% to 1%)
• Uncommon among humans
• Common in institutionalized patients (in overcrowded institutions)
• Areas with poor sanitation
• People at risk: those in close contact with pigs or pig feces
• Warm and humid climates in tropical and subtropical countries can contribute to cyst survival
Treatment and • Metronidazole, Iodoquinol Tetracycline
prevention • Avoid using pig feces as fertilizer
BLASTOCYSTIS HOMINIS
Blastocystis hominis
• Classified member of Stramenopiles
• Suggested new class: Class Blastocystea
• Previously classified as a yeast in Schizosaccharomyces
• Also previously associated with Blastomyces
• MOT: ingestion of thick walled cysts
• Life cycle still not fully understood
Morphologic Forms • Classic Vacuolated Form
(Central-Body Form): most
predominant
o Large central vacuole
pushes the cytoplasm
to the periphery
• Granular forms: multinucleated
• Multivacuolar
• Avacuolar
• Ameboid form: exhibit active
extension and retraction of
pseudopodia
o Nuclear chromatin
exhibits peripheral
clumping
o Intermediate stage
between vacuolar and
precystic form
• Cyst: has a thick, osmophilic, and electron dense cystic wall
Disease Manifestation • Blastocytosis
• Pathology is still in question and controversial
• Diarrhea, nausea, anorexia
• May also be associated with irritable bowel syndrome
Epidemiology • Occurs worldwide
• Zoonotic
• Most common subtype infecting man is subtype 3
Lab Diagnosis • DFS
• FECT
• Molecular methods
• Stains
• Culture: Boeck and Drborhlav
Treatment • Still controversial
• Usually use metronidazole
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MALARIAL PARASITES
• Hematin: pigment found in Plasmodium species as a result of the parasite feeding on hemoglobin
Plasmodium falciparum
• Most prevalent Plasmodium in the Philippines
• Merozoites develop in the parasitophorous vacuolar membrane (PVM)
o Inside RBC
o Modify structural and antigenic properties of RBC
• Once merozoites invade RBCs, RBCs reduce their deformability
o Due to changes in red blood cell cytoskeleton and increase in membrane stiffness and cytoplasmic viscosity
Type of Malaria Malignant Tertian Malaria
*or Subtertian Malaria or Estivoautumnal Malaria
Paroxysmal Cycle 36-48 hours
Type of RBC infected All forms (high rate of infectivity)
Size of Parasitized RBC Normal
Presence of RBC stages Ring forms, gametocytes (developing trophozoite is rare)
Ring Forms • Delicate small ring
• May have 2 chromatin dots (indicating
multiple infection)
• Common multiple rings in an RBC
o Headphone/smiley
o Accole/applique: chromatin in
periphery
o Exclamation point
o Question mark
Plasmodium vivax
• Most prevalent species (widest distribution)
• Infections usually benign (targets only young RBCs, with ovale)
o Relapses can occur (but this is a case-to-case basis)
▪ Renewed asexual parasitemia
▪ Disease manifests again after many years
▪ Do not need to get bitten again for relapse
▪ Reactivation of hypnozoites
▪ Factors that promote reactivation
• Stress
• High fever
• Pregnancy
• Depression
• Immunocompromised patients
▪ Exo erythrocytic schizogony
o Vivax more severe when compared to ovale
• Incubation period: 8-17 days
• Pre-patent period: 11-13 days
• Pre-erythrocytic stage: 6-8 days
• Anemia is mild to moderate
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Plasmodium ovale
• Infections usually benign (targets young RBCs only, with vivax)
o Spontaneous recovery can occur after 6-10 paroxysms
o Relapses can also occur
• Presence of 2 distinct nonrecombining species
o Classic: Plasmodium ovale curtisi
o Variant: Plasmodium ovale wallikeri
• Infections may no longer be limited to areas of Tropical Africa, Middle
East, Papua New Guinea, and Irian Jaya (Indonesia)
• Incubation period: 10-17 days
• Pre-patent period: 11-13 days
• Pre-erythrocytic stage: 9 days
• Mild anemia
• Possible CNS involvement
• Rare nephrotic syndrome
• No recrudescence
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Plasmodium malariae
Type of Malaria Quartan Malaria
Paroxysmal Cycle 72 hours
Type of RBC infected Old RBCS (Senescent RBCS)
Size of Parasitized RBC Normal
Presence of RBC stages Few ring forms seen (mostly trophozoites and schizonts)
Ring Forms • Heavy chromatin dot (bird’s eye appearance)
• Small form
Plasmodium knowlesi
• mostly in Southeast Asia (Malaysia, Indonesia, can also be seen in Palawan)
• almost the same morphology with malariae
• differentiated through molecular methods (PCR) and molecular characterization
• parasite of long-tailed macaques (Macaca fascicularis)
Type of Malaria Simian Malaria, Quotidian Malaria
Paroxysmal Cycle 24 hours (has the shortest erythrocytic cycle)
Type of RBC infected Can infect all
Size of Parasitized RBC Normal
Ring Forms • Early ring form: like falciparum
• Later ring form: like malariae
Developing Trophozoite Band formation
VECTORS
Mosquito Description
Anopheles minimus • Primary mosquito vector in the country
flavirostris • Only female bites (for egg nourishment and ovulation, males only go to flowers)
• Night biter (10pm – 2am)
• Bites indoors and outdoors
o Exophagic: likes biting outdoors
o Endophagic: likes biting indoors
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• Anthropophilic (likes biting humans) and zoophilic (also likes biting animals)
Anopheles maculatus • Transmits malaria in hilly or high altitudes
• Mountainous areas
• Zoophilic
• exophagic
Anopheles litoralis • transmits in coastal areas
• larvae breeds in salt or brackish (mix of fresh and salt water) waters
• anthropophilic
Anopheles mangyanus • transmits in forest-fringe areas (edge of the forest)
• similar breeding with flavirostris
Anopheles balabacensis • transmits in forests
• breed in stagnant water, hoof prints (when water goes inside the prints), dug wells
• anthropophilic
• secondary vector of malaria in the country
Anopheles gambiae • primary mosquito vector in Africa
• best vector of malaria
o bites humans only
o longer life span compared to other species
o more time to bite people
o Spontaneous abortion
o Still birth
o Low birth weight associated with risk for neonatal
death
• Children
o Cerebral malaria, seizures, neurologic sequelae,
opisthotonos
• Severe
o Impairment of consciousness and other signs of
cerebral dysfunction (delirium and generalized
convulsions)
o Severe hemolytic anemia
o hyperbilirubinemia
PATHOGENESIS
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N. Villanueva
• Orange: stains all malarial parasites (because the o Resistance has been reported
parasites have nucleic acids, and normal RBCs do not • Arthemether combination treatment like arthemether
have those) lumefantrine (coartem)
o WHO recommended drug for falciparum and malariae
RAPID DIAGNOSTIC TESTS (RDTs) o Combination therapy
• Quinine: severe malaria and DOC for pregnant women
• Immunochromatographic Mtds
• Artesunate – severe malaria
• Antigen detection
o HRP-II: histidine rich protein (Antigen produced by • Primaquine: relapsing vivax or ovale malariae
Falciparum trophozoite and gametocytes) • Tetracycline
▪ Paracheck PF, ParaHIT f • Doxycycline – not given to women and children (target
▪ Specific for Falciparum the bones)
o pLDH: parasite lactate dehydrogenase (produced • Tissue schizonticides: act on pre-erythrocytic forms
by viable parasites) • Gametocytocidal drugs: destroy sexual forms of parasite
▪ can distinguish Falciparum from non-Falciparum in the blood
species • Hypnozoitocidal/antirelapse: kill dormant forms in liver
▪ ex: Optimal brand • Sporonticidal drugs: inhibit development of oocysts on gut
o Aldolase: other panmalarial antigens wall of mosquito
▪ Can be positive for all malarial agents except for
knowlesi (can only distinguish it using molecular PREVENTION
methods)
• Early diagnosis
OTHER METHODS • Prophylaxis
• Use of insecticide treated nets and repellants (target
• Serology (ELISA, IHA, IFAT) vector)
• Molecular (PCR for low parasitemia and mixed infections) • Use of larviparous fish (prevent transmission)
• Culture: RPMI 1640 • Use of larvicides (kill larva)
o Ex: Bacillus thuringensis (secretes toxin that kills
TREATMENT larva)
• Chloroquine: main treatment • Health education
o DOC for uncomplicated P. falciparum, vivax, • No clear vaccine
malariae, and ovale
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N. Villanueva
INTESTINAL COCCIDIANS
CRYPTOSPORIDIUM
Asexual and Sexual Life Cycle
• Sexual and asexual cycle happens only in one host (no
intermediate hosts)
Sporulated oocyst • goes to stomach
(contains • then goes to small intestine
sporozoites which
are sausage-
shaped) is
ingested
Sporulated oocyst • 1 sporulated oocyst = 4 sporozoites
release • Sporozoites infect columnar cells of small
sporozoites in intestine
small intestine o Only target upper portion of columnar cells
o Usually only found in microvilli or brush border
• Site of infection: outside cytoplasm of the cell
o extracytoplasmic
Sporozoites • Type 1 Meront
become o Asexual reproduction (merogony)
trophozoites then o Contains merozoites
trophozoites o Meront ruptures to release the merozoites
become meronts o Merozoites infect other intestinal cells
o After many cycles, some merozoites become
Type 2 meront
• Type 2 Meront
o Sexual reproduction (sporogony/gametogony)
o Release merozoites
o Merozoites become gamonts (sexual cells)
▪ Macrogamont
▪ Macrogamont
o Microgametes and macrogametes released
▪ Fuse to form zygote
o Zygote eventually becomes an oocyst
(already sporulated, already has 4
sporozoites inside)
• Two types of oocyst
o Thin-walled oocyst
▪ Remains in small intestine
▪ Associated with autoinfection (deadly
among immunocompromised)
▪ Eventually ruptures and releases
sporozoites inside the small intestine
o Thick-walled oocyst
▪ This type is the one seen in the stool
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N. Villanueva
Cryptosporidium spp.
• Cryptosporidium hominis
• Most common species infecting man
• Formerly known as Cryptosporidium parvum antroponotic genotype (but they found out this does not usually
infect humans, usually infects animals)
• Infective stage: ingestion of sporulated oocyst
• Habitat: small intestine (jejunum)
• Target cells: enterocytes (intestinal cells, columnar cells with microvilli, brush border)
• Low infective dose (important cause of outbreak of diarrhea, ingest around 10 cysts only)
• Large multiplication capability: because of the autoinfection caused by the two types of cysts
Mode of • Ingestion of sporulated oocyst
Transmission • Drink contaminated water
• Swim in recreational pools that are fecally contaminated (accidentally drink the water)
Disease Healthy Immunocompetent Patients
Manifestation • Watery diarrhea (5-10 frothy bowel movements)
• Usually self-limiting (disappears in 2-3 weeks)
• Important cause of outbreaks of diarrhea (can be considered as a bioterror agent)
Immunocompromised patients (AIDS patients)
• Chronic diarrhea
• Extraintestinal infections
• Severe and life threatening
• Immune system is weak, so they cannot control the parasites
• Severe dehydration, electrolyte loss, excessive fluid loss
• Chronic respiratory infections may also occur (pneumonia, dyspnea, bronchiolitis, chronic cough)
• Cholecystitis: affecting bile ducts of the gall bladder
Pathology • Changes in the morphology of the villi
o Becomes blunted and infiltrated by inflammatory cells
o Important for absorption of nutrients
o Atrophy of villi (becomes smaller)
Diagnosis • Preferred sample: stool
o More watery: better (high detection rate)
• Concentration techniques
o Sheather’s Sugar Floatation
▪ Same principle as brine floatation
▪ But you use sucrose solution
▪ Better than FECT because Cryptosporidium is small (FECT involves
sedimentation)
o FECT
• Fecal smear using Modified Kinyoun Method (fastest and cheapest)
o Because the organism is partially acid fast
o Modify a step in the staining procedure: the decolorizer (use 1% H2SO4 instead of 2-3%
HCl and 95% alcohol found in acid alcohol)
▪ No more alcohol because it is strong to the organism (might kill it)
o Find circular structures that are color red
o Size of oocyst is important: 4-6 um (important diagnostic feature)
• Duodenal aspirates
• Enterotest
• Molecular methods
• Serologic tests
• Fluorescent technique
Epidemiology • Found worldwide
• Zoonotic infection
• Implicated in outbreaks (children at risk)
• Infective upon release
• Highly resistant to disinfectants
o not killed by chlorination
Treatment • no standardized treatment (because if you’re healthy, it will go away after 2 weeks)
• can give Nitazoxanide
• immunocompromised patients: improvement of immune status
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N. Villanueva
CYCLOSPORA CAYETANENSIS
Cyclospora cayetanensis
• Species name refers to Cayetano Heredia University in Lima, Peru (where
epidemiological and taxonomic work was done)
• Formerly classified as CLB (cyanobacterium like body)
• Appearance almost the same as Cryptosporidium
o Bigger size: 8-10 um
• Life cycle almost the same as Cryptosporidium
o Infective stage: sporulated oocyst
o Slight change in morphology of sporulated oocyst (presence of
sporocyst)
o Sporocyst: contains 2 sporozoites inside
o 4 sporozoites in 1 oocyst
o Oocyst released is unsporulated (not infective)
o Morula formation: undeveloped/undifferentiated structures inside the
unsporulated oocyst
o Sporulation happens in the environment (5-10 days)
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N. Villanueva
CYSTOISOSPORA BELLI
Cystoisospora belli
• Formerly known as Isospora belli
• Least common infecting man
• Largest oocyst
• Infective stage: sporulated oocysts
o Oval-shaped, 2 sporocysts with 4 sporozoites each (total of 8 sporozoites
per sporulated oocyst)
• Life cycle almost the same with Cryptosporidium
o Release unsporulated oocyst
o Sporoblasts: undifferentiated structures inside
o 48 hours for sporulation to take place in the environment
TISSUE COCCIDIANS
TOXOPLASMA GONDII
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N. Villanueva
Toxoplasma gondii
• Parasite of cats
• Complete life cycle happens in cats
• Humans: intermediate host only (accidental)
o We can also be dead-end host (life cycle stops once in our bodies)
• Infective stage: sporulated oocyst or tissue cysts
Mode of transmission • Ingestion of sporulated oocyst or tissue cysts
o Oocyst: ingestion of cat feces
o Tissue cysts: ingestion of contaminated meat (can be rat meat, undercooked, raw
meat), organ transplants
• Eating contaminated food/drink with feces of cat
• Vertical transmission (especially if mom is infected during pregnancy
• Eating cats (possible)
Diagnostic stage Tissue cysts
• Bradyzoites
o Multiply slowly
o Develop mostly in neural and muscular tissues
o May also develop in visceral organs
o Late stages of infection
o Enclosed in a tissue cyst
• Tachyzoites
o Rapidly multiply
o Infect cells of the intermediate hosts and non-intestinal epithelial cells of cats
o Found in early stages of infection
o Crescent-shaped
Disease manifestation and • Infections are usually asymptomatic (in immunocompetent individuals)
Pathogenesis o May exhibit flu-like symptoms
o People: almost all of us are actually exposed or positive for the parasite
▪ Most people would have antibodies against Toxoplasma
• Immunocompromised patients (AIDS patients)
o Lead to encephalitis
o Formation of multifocal brain lesions
o May lead to blindness (can affect the eyes)
o Retinochoroiditis
o Lymphadenopathy
o Splenomegaly
• Ocular infections (chorioretinitis)
• Transplant patients: multiorgan failure
• Congenital infections
o Stillbirth, abortion
o Triad of toxoplasmosis (if baby lives)
▪ Hydrocephalus
▪ Chorioretinitis
▪ Intracranial calcification (calcium deposits on brain)
o Microcephaly may also occur
o TORCH Test (screening during pregnancy)
▪ Toxoplasmosis
▪ Other infections (coxsackievirus, chickenpox, chlamydia, HIV, human T-
lymphotropic virus, syphilis)
▪ Rubella
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N. Villanueva
▪ Cytomegalovirus
▪ Herpes simplex
Pathogenesis • Obligate intracellular parasites (invade nucleated cells including macrophages)
• infected cells rupture leading to dissemination
• mostly asymptomatic among healthy people (because of humoral and cell mediated
immunity)
Diagnosis • usually biopsy
o examine tissues and look for bradyzoites and tachyzoites
o difficult and invasive
• preferred method: Serology
o detect antibodies
o Sabin-Feldman Test (uses methylene blue, most important, classic method)
▪ Sensitive and specific
▪ Specimen: serum sample
▪ Reagent: Live Toxoplasma
▪ Mix serum with Live Toxoplasma, antibodies in the sample will form
immune complexes with the reagent
▪ Add methylene blue (complex prevents binding of methylene blue)
▪ Positive result: non-uptake of the dye (colorless)
▪ Negative result: blue color
▪ Titer: highest solution of antibody
• High titer: >1024 indicates acute infection
o Enzyme immunoassay
o Hemagglutination test
o FAT
o TORCH testing
• Culture: use of vero cell lines and test animals
o Test animals: mice, gold hamster (gerbil, infect them and if they develop the
disease, the person is positive)
• Examination of giemsa stained tissue sections or imprints
• Examination of CSF smears, buffy coat smears
• Molecular methods
Epidemiology • Worldwide distribution (majority are seropositive)
• People at risk of severe toxoplasmosis
o Infants born to exposed mothers (during pregnancy)
o Immunocompromised
Treatment Pyrimethamine and Sulfadiazine
Prevention • Thoroughly cook meat
• Proper hygiene
• Disinfect and clean daily cat litter pans
• Pregnant women: avoid cats
• Avoid cats
SARCOCYSTIS SPP.
Sexual Cycle
In final host, • Zoites infect and produce
sarcocyst gametes (microgametes and
releases zoites macrogametes)
• Zygote formed
• Zygote forms oocyst
(sporulated in human host)
• Sporulated oocyst: contains
2 sporocysts, each with 4
sporozoites (total of 8)
Sporulated oocyst • Ingested by intermediate
released in feces host
Asexual Cycle
Sporozoites form • Merozoites form 2nd and 3rd
merozoites generation meronts
• Meronts form sarcocyst
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N. Villanueva
Sarcocystis spp.
• Infects a wide variety of animals and sometimes humans
• S. hominis: involves cattle
• S. suihominis: involves pigs (-suis: related to pigs)
• Final host: humans
• Intermediate hosts: pigs and cattle
• Infective stage to final host: sarcocyst (tissue cysts found in muscle and tissues of pigs and cattle)
o Sarcocysts contain zoites
• Man can sometimes be an intermediate host (accidentally ingest sporulated oocyst)
o Dead-end host
Mode of transmission • Ingestion of infected meat
Disease Sarcosporidiosis or Sarcocystosis
• Invasive form (rare)
o Accidentally ingest sporulated oocyst
o Vasculitis
o Myositis: inflammation of heart muscle
o If we become intermediate host
• Intestinal form (more common)
o Human: final host
o Nausea, abdominal pain, and diarrhea
o Usually mild, less severe (for 48 hours lang)
o Self-limiting
• Other manifestations
o Acute fever, myalgia, bronchospasm, elevated ESR, elevated Creatine Kinase enzyme
(elevated in muscle pain), symptoms may last up to 5 years
Diagnosis • muscle biopsy (definitive diagnosis)
o sarcocysts: microscopic in cattle (S. hominis)
o sarcocysts: macroscopic in pigs (S. suihominis)
▪ stain: H&E; PAS (confirmatory)
• stool exam: detection of sporocyst
o concentration methods: floatation
• PCR
Treatment • Rarely required (because asymptomatic)
• May use albendazole, metronidazole, co-trimoxazole
Prevention • rare in humans
• thorough cooking of meat
• freezing of meat (low temp kills sarcocysts)
TRYPANOSOMA CRUZI
Human Stages
Triatomine bug • Triatomine bug
takes a blood o Female
meal o Also known as Assassin bug
o Bites during the night
o Also known as kissing bug because they
prefer to bite in mucosal areas or in the lips
Triatomine bug • Infective stage to humans: feces of the bug
defecates on o Metacyclic trypomastigote
the wound o Feces enters bite wound (it is not injected)
Metacyclic • Metacyclic trypomastigote becomes an
trypomastigote amastigote inside host cells
penetrates • Amastigotes reproduce asexually (binary
different kinds fission)
of cells
Host cells • Amastigotes transform into trypomastigotes
release (diagnostic stage)
amastigotes • Enter the bloodstream
• Trypomastigotes infect other host cells
• Become amastigotes again inside the host
cells
• Clinical manifestations can arise from this
cycle
Triatomine Bug Stages
Triatomine bug • Acquires trypomastigote (infective stage to
bites human the bug)
In midgut of • Trypomastigotes become epimastigotes
triatomine bug (via longitudinal fission)
• Multiply
In hindgut of • Epimastigotes become metacyclic
triatomine bug trypomastigotes
• Fast transformation
o That’s why promastigote sometimes not
presented (but the stages happen in the
insect)
• Triatomine bug bites human, transfers
metacyclic trypomastigotes via feces
• Amastigote in tissue specimens:
intracellular
• Trypomastigote in blood: extracellular
Trypanosoma cruzi
• All four morphological forms are found
• Belongs to Trypanosome Group Stercoraria
• Primarily infects: myocytes, heart cells, and RESs (reticuloendothelial system: monocytes, macrophages, skin, gonads,
intestinal mucosa, placenta etc., so it is intracellular)
• Causes Chagas’ Disease (Dr. Chagas first to study the disease) or American Trypanosomiasis (because of high prevalence
in America)
• Found in the PH (but no cases)
o Found in squalid areas or dirty areas, mud walls
• Infective stage to humans: metacyclic trypomastigote
• Multiply within the mammalian host in a discontinuous manner
• Zoonotic mammalian reservoir hosts: domestic animals, armadillos, raccoons, rodents, marsupials, and some primates
Mode of transmission • Feces of vector entering bite wound
• Blood transfusion
• Transplacental (vertical, can cross placenta during pregnancy)
• Transmission associated with poor living conditions
Final Host Humans
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Intermediate Reduviid Bug/Kissing bug (Triatoma, Panstrongylus, Rhodnius)
host/Vector
Disease Manifestation Acute phase (Initial)
• Fever and lymphadenopathy (enlargement of lymph nodes near the chagoma)
• Diffuse or focal inflammation (affecting myocardium)
• Malaise
• Nausea
• Vomiting
• Chagoma: local inflammation, reddish nodule, furuncle-like lesions associated with central
edema, regional lymphadenopathy (at site of bite wound)
• Romaῆa’s sign: periorbital swelling (edema of eyelid and conjunctiva) parasite penetrates the
conjunctiva, unilateral swelling (only one eyelid affected), bipalpebral edema, conjunctivitis
• after a few months, symptoms disappear (latent phase)
Chronic Phase (after 10-20 years)
• no characteristic symptoms
• during this phase, still capable of transmitting it to other people
• amastigotes still reproducing
o triggers enlargement of vital organs
o fibrotic reactions that can cause injury to the myocardium, cardiac conduction network,
and enteric nervous system (decrease in nerve ganglia, leading to megasyndromes)
o congestive heart failure
o thromboembolism
o chest pain, palpitations, dizziness, syncopal episodes, abnormal ECG findings
o mega colon (chronic constipation)
o mega cardium/cardiomegaly (can develop arrhythmias and you can die)
o mega esophagus (achalasia)
*majority of symptomatic chronic patients manifest with the cardiac form, rest with gastrointestinal form
Pathogenesis • acute inflammatory reaction on bite (Chagoma)
• uses lectin like carbohydrates for binding
• target cells: cells of RES, cardiac cells, skeletal and smooth muscles, neuroglia cells
Diagnosis • complete patient history
o determine possible exposure, risk factors, recent transfusion, contact
o primary tool
• presence of lesions (in early phases)
o aspirate, prepare a smear, stain, then view
• cardiac symptoms present, especially if living in endemic regions
• demonstration of trypanosomes in:
o blood (thick and thin smears) for sdefinitive diagnosis
o buffy coat (concentration technique: Strout Method), can see trypomastigote and
sometimes the amastigote
o CSF
o Tissues (can see amastigotes)
o Lymph
o Trypomastigotes only seen in first two months of acute disease
• Concentration methods (microhematocrit)
• PCR
• Cardiac form
o ECG and echocardiography (may show atrial fibrillation/flutter, low QRS voltage, dilated
cardiomyopathy, and tricuspid and mitral regurgitation)
• Intestinal form
o Barium esophagogram (esophageal dilation)
o Barium enema (megacolon of the sigmoid and rectum)
• Xenodiagnoses
o Use of kissing bug (make the bug bite you)
o If parasite develops inside, then you are infected
• Culture: Chang’s, NNN
• Serology: IFAT, Complement Fixation (Guerreiro Machado Test), ELISA, Western Blot, IHA (for
chronic phase)
o WHO recommends using at least two techniques with concurrent positive results before
making a diagnosis of Chagas disease
Epidemiology • Occurs only in the American continent
• Highest prevalence in Brazil
• More common in rural areas (because they prefer squalid conditions, mudwalls)
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N. Villanueva
• Chronic disease more common
• Common in unsanitary housing conditions
Treatment Nifurtimox and Benznidazole
*symptom-specific management
Prevention • Vector control
• Screening of blood
• Health education
Trypanosoma rangeli
• Nonpathogenic
• Metacyclic trypomastigote is discharged via the salivary glands
o Injected
o Not in the feces
Vector • Rhodnius
Human stages
Tsetse fly bites • Takes a blood meal
human • Injects metacyclic
trypomastigotes
Injected • Goes to different parts of the
metacyclic body (brain and other vital
trypomastigotes organs)
transform into • Trypomastigotes multiply
bloodstream (binary fission) in various
trypomastigotes body fluids (blood, lymph,
(diagnostic stage) spinal fluid)
Trypomastigotes • Tsetse fly bites human,
found in blood acquires the trypomastigotes
Tsetse fly stages
Tsetse fly’s • Trypomastigotes become
midgut procyclic trypomastigotes
• Procyclic trypomastigotes
multiply by binary fission
Procyclic • Transform into epimastigotes
trypomastigotes
leave midgut
Epimastigotes go • They multiply here
to salivary glands • Transform into metacyclic
trypomastigotes
• Tsetse fly bites human and
injects the metacyclic
trypomastigotes
LEISHMANIA
Human stages
Sandflies bite human • Injects promastigote into the skin
Promastigotes • Promastigotes become
ingested/phagocytized amastigotes inside the
by macrophages in the macrophage
blood • Amastigotes multiply inside
Macrophages burst • Release amastigotes
• Amastigotes: infective stage to
sandflies
Sandfly stages
Sandflies bite human • Ingests macrophages infected
with amastigotes
In midgut of sandfly • Amastigotes transform into
promastigotes
• Amastigotes divide in the midgut
Amastigote migrate to • When it bites a human, it injects
proboscis the promastigotes
Leishmania spp.
• Vector borne parasitic disease
o Sandflies: Phlebotomus spp. (infects old world, Europe), Lutzomyia (infects new world, US)
• Intracellular parasites (inside host cells)
• Diploid protozoa
• Zoonotic (dogs in urban places, rodents in urban and rural places)
• Old World Leishmaniasis: L. tropica (Asia and Eastern Europe), L. aethiopica (Africa), L.major
• New World Leishmaniasis (Mexico, Central America, South America, Amazon rainforest): L. mexicana, L. amazonensis,
L. guyanensis, L. braziliensis, L. chagasi
• Leishmania tropica
• Leishmania braziliensis
• Leishmania donovani (most severe)
• Infective stage to humans: promastigotes
• Infective stage to sandflies: amastigotes
• Targets RE cells
• Viannia subgenus produces promastigotes in the hindgut, midgut, and proboscis (Leishmania subgenera only produces
promastigoes in the midgut and proboscis)
Mode of transmission • Bite of vector
• Blood transfusion
• Close contact
• Contamination of bite wounds
Intermediate host/Vector Sandflies
Final Host Humans
Disease Manifestation: • Also known as Old World Leishmaniasis, Aleppo Button, Delhi Boil, Baghdad Boil, Jericho Boil
Cutaneous • Etiologic agent: Leishmania tropica (can also be caused by L. major and L. mexicana)
Leishmaniasis o Live in skin capillaries (in the endothelial cells)
o That’s why they’re seen as lesions on the skin
o L. tropica: dry or urban oriental sore
o L. major: moist or rural oriental sore
o L. mexicana: chiclero ulcer
• Incubation period: weeks to months
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• Painless elevated skin ulcers
o Leaves an ugly scar and is highly disfiguring (erodes the skin)
o Oriental Button: erythematous papule which forms an ulcer
• Common in Middle East and some parts of Asia
Diffuse Cutaneous • AKA Anergic or Lepromatous Leishmaniasis
Leishmaniasis • Characterized by a localized, non-ulcerating papule
• Develops diffuse satellite lesions (affects the face and extremities)
• May be initially diagnosed as lepromatous leprosy
Disease Manifestation: • Also known as American, New World Leishmaniasis
Mucocutaneous • Etiologic agent: Leishmania braziliensis
Leishmaniasis • Incubation period: weeks to months
• Habitat: tissues in nose and mucous membranes
• Initial: ulcers are similar to Cutaneous Leishmaniasis
• Later stage: spreads to oronasal and pharyngeal mucosa (can lead to dysphonia, dysphagia,
and aspiration pneumonia)
o Involvement of the mucous membranes results in nasal stuffiness, discharge,
epistaxis, and destruction of the nasal septum
o Espundia: in the nose
o Tapir Nose: also affects the nose
o Chiclero Ulcer: affects ears
Disease Manifestation: • Also known as Kala-azar, Dumdum Fever (this is a place), Black Fever
Visceral Leishmaniasis • Etiologic agent: Leishmania donovani (can also be caused by L. chagasi and L. infantum)
• Incubation period: 1-3 months
• Habitat: RES
• Dromedary fever peak: fever with twice daily elevations (Double Quotidian)
• Splenomegaly
• Cachexia
• Reticuloendotheliosis
• Hepatomegaly
• Darkening of skin (forehead, temples, around the mouth)
o That’s why its called black fever
• Dermal leishmanoid lesions (rare)
o Present if treatment is incomplete
• Post-kala azar dermal leishmaniasis (PKDL): sequela
o Cutaneous eruption resulting in hypopigmented macules, malar erythema, nodules,
and ulcerations
o Manifest a few months to several years after treatment
Epidemiology • Endemic in 88 countries on 5 continents
• Visceral Leishmaniasis
o Bangladesh, Brazil, India, Nepal, and Sudan
• Cutaneous Leishmaniasis
o Afghanistan, Brazil, Iran, Peru, Saudi Arabia, Syria
• Mucocutaneous Leishmaniasis
o Brazil, Eastern Peru, Bolivia, Paraguay, Ecuador, Colombia, Venezuela
Diagnosis • Demonstration of lesions
• Tissue biopsies (for amastigotes)
• Skin biopsies (for amastigotes)
• Animal inoculation
• Examination of bone marrow, spleen, lymph node (also for amastigotes)
• Montenegro Skin Test
o Also called Leishmanin Skin Test
o Test to determine if you have a previous exposure to the parasite
o Person injected with a suspension of parasites (promastigote) in the intradermal area
o Positive result: if there is enlargement
o Negative in diffuse cutaneous leishmaniasis and kala azar
• Formol Gel Test
o Useful for donovani
o To determine if there is hypergammaglobulinemia
• Serology: IFAT, ELISA, rk39 antigen dipstick test
• Culture: NNN (Schneider’s medium also found useful)
• Molecular methods
Treatment • Antimony compounds (sodium stibogluconate, n-methyl-glucamine antimonite or meglumine)
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N. Villanueva
BABESIA
Erythrocytic cycle
Sporozoites • start of erythrocytic schizogony
infect RBCs • no exo-erythrocytic cycle in Babesia spp.
• sporozoites develop into an immature
trophozoite form
• trophozoites develop into merozoites
Merozoites • infects other RBCs
released from
RBCs
Gametogony • after many cycles, gametes are produced
(macrogamete and microgamete)
• no schizonts and gametocytes
Transmission of • gamete is ingested by another tick
gametocytes • gamete: infective stage to the definitive host
Sporogonic cycle
Gut/GI Tract • macrogamete and microgamete fuse to form
zygote
• fertilization
Formation of • zygote becomes elongated and motile
ookinete o forms ookinete
o subsequent development of Babesia:
development of numerous kinetes
(sporokinetes)
▪ when sporokinetes are released, they
continue to infect and multiply in
various organs and the ovaries, until
death ensues
• ookinete enters salivary gland
• ookinete develops into an oocyst
o sac-like structures with sporozoites
Oocyst bursts • releases sporozoites
• already in the salivary glands
Babesia spp.
• Blood-borne and vector-borne
o Transmitted by ticks (genus Ixodes)
▪ Ixodidae or hard ticks
▪ Transmission via soft tick (Ornithodoros erraticus) has been reported
▪ Other vectors: Boophilus spp., Rhipicephalus spp., Hyalomma spp., Haemaphysalis spp., and Dermacentor spp.
• Hemosporidian parasites
• Dr. Victor Babes: first documented Babesia in cattle (1888)
• Heteroxenous parasite: infesting more than one kind of host, requiring at least two kinds of host to complete the life cycle
(mammal as primary host and ticks as intermediate hosts or vectors)
• has a tendency to take on pleomorphic forms (ability to alter their shape or size in response to environmental conditions) in
different hosts
o obscures their identification at the species level
• transstadial: capable of stage-to-stage passage
o each of developmental stages capable of parasite transmission to mammals
• smaller forms (ex: Babesia bovis and Babesia equi more pathogenic
• larger forms (ex: Babesia bigemina and Babesia caballi) less virulent
• Sometimes mistaken for Plasmodium
o Important to differentiate them
o Babesia: blood parasite that causes malaria-like infections
o Babesia does not undergo exoerythrocytic merogony (residual bodies usually not found in infected RBCs)
• Zoonotic infection
• Causative agents: Babesia microti (found in the Northeastern US) and Babesia divergens (found in Europe)
o Groupings obtained through phylogenetic analyses of gene sequences of SSU rDNA (small subunit ribosomal
deoxyribonucleic acid) of Babesia spp.
Mode of • Bite of an infected tick (blood meal)
Transmission • Blood transfusion
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N. Villanueva
• Vertical transmission
• Organ transplant
• Transovarian transmission (for Babesia divergens)
o Ticks can pass the parasite to its progeny/offspring
o Infect ova (egg) of the ticks
o Terminates with death of the vector
Definitive Host Tick (Ixodes)
Intermediate Host Mammals
• White footed mouse (most important)
• livestock
• Cattle
• Humans (accidental intermediate host only, because this is primarily a zoonotic infection)
• Deer: primary reservoir host
Infective stage to intermediate hosts: sporozoites (called pyriform bodies)
Morphology Similar to malarial parasites
• But no schizonts or gametocytes
• Up to four trophozoites found in each cell (sometimes mistaken as Falciparum)
• Presence of up to four merozoites
o Maltese Cross Arrangement (four merozoites)
o Bunny Ears/Rabbit Ears Appearance (two merozoites)
Disease • Causes Babesiosis, Piroplasmosis, Nantucket Fever, Splenic Fever, Redwater Fever, Tick fever,
Manifestation and or Texas Cattle Fever (first identified among cattle)
Pathogenesis • Most cases are asymptomatic and usually self-limiting
o Healthy people have no symptoms (if ever there is, it is only mild and self-limiting)
o In low-grade and chronic infections
• Incubation period: 1-12 months (wide range)
• No paroxysmal cycle
• 1-6 weeks postexposure: fatigue, malaise, anorexia, and weight loss
o Followed by nonperiodic intermittent fever, chills, sweats, headache, myalgia, arthalgia,
nausea, vomiting, and prostration
• Signs and symptoms mimic malaria
o Mild chills and fever
o Hemolytic anemia
▪ With hemoglobinuria, jaundice, pulmonary edema in severe cases
o Jaundice
o Hepatomegaly
o Hepatosplenomegaly (because lots of RBCs are destroyed, and they go to the spleen)
• Patient may also manifest emotional lability, depression and hyperesthesia
Risk Factors • Most people have no problem with this (immunocompetent)
• Affects primarily the elderly, immunocompromised, splenectomized patients (those that underwent
splenectomy, because they cannot remove defective RBCs)
• Co-infection with Borrelia burgdorferi (Lyme disease)
o Both share same tick/vector
o Same endemic area
o Same manifestations
Immunity • Both innate and acquired immunity contribute to resolution of the primary infection (provide
protection against subsequent exposures)
Diagnosis • Blood smears: for acute infections only
• Examination of Giemsa stained smears
o Definitive diagnosis
o Shows unique morphological features
• Parasite dimensions and pleomorphisms are noted
o Ring form, pear-shaped, Maltese cross or tetrad form
o Need to be ascertained (with absence of pigment in infected RBCs)
o To rule out misdiagnosis from Plasmodium and Lyme disease
• Serology
o IFAT (diagnostic titer or amount of antibody present = 1:64)
▪ Indirect fluorescent antibody test
▪ Serological method for confirming the identity of viral isolates
o Immunofluorescent assay (IFA)
▪ Widely used in acute cases and epidemiological studies
o Inoculation of animals (Gold hamster or gerbil)
▪ If the animal dies or gets the disease, then they test positive
▪ For cases of low parasitemia
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N. Villanueva
o In cases of low-grade infection or parasitemia
o Serum dilution
▪ To rule out possible cross-reactions
• History of tick bite
• Molecular methods (sensitive but expensive)
o In cases of low-grade infection or parasitemia
o PCR: gold standard for Babesia detection
Epidemiology • Zoonotic
• Reported in Europe, North East North America, and also in the West Coast
• Human infections usually occur during spring or summer
• Affects farmers living in close habitation with livestock and wild animals
• No human infections reported yet in PH (but there are animal cases)
• Epidemiologic data useful for low-grade infection or parasitemia
• B. microti and B. divergens
o Third grouping: the WA1-type in Western US (tentatively grouped with B. microti or with
Theleria spp.)
• Recently in Italy and Austria: parasites revealed SSU rDNA sequences more closely related to B.
odocoileus (species has morphological, molecular, and immunological similarities to B. divergens)
Treatment • Combination of Clindamycin and Quinine
• Or Azithromycin and atovaquone
• For B. equi and B. caballi in vitro
o Use arteminisin, pyrimethamine, and pamaquine
Prevention • Avoid tick bites
• Apply insect repellants
• Screen blood donors
• Remain covered with clothing
• Immediately remove any attached ticks
• Control rodent population (since they are major carriers or reservoirs)
MICROSPORIDIA
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N. Villanueva
NEMATODES
ASCARIS LUMBRICOIDES
Life Cycle
Ingestion of embryonated egg • Goes to stomach then to the small
intestine
• Larva emerges from the egg
Larva undergoes heart-lung • Larva penetrate small intestine, goes
migration to heart and lungs
• Important for the development of the
parasite
• After migration, goes back to
esophagus to be swallowed back into
the small intestine
Becomes adult worm in small • Undergo sexual reproduction
intestine (produce eggs)
• Unembryonated eggs found in stool
sample
Egg should find its way to the • Embryonation in the soil takes 2-3
soil in order to become weeks
embryonated *if parasite undergoes heart-lung migration, life
*preferred soil is loamy (+moist cycle is classified as an indirect life cycle
environment)
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N. Villanueva
Ascaris lumbricoides
• Common name: Giant Intestinal Roundworm
• Infective stage: embryonated egg
• Ascaris of humans (primarily infects humans only)
o No known reservoir hosts
• Habitat: small intestine
• Final host: man
• Soil-transmitted helminth
• Normal life span: 1 – 2 years
• Do not multiply in the small intestine
• Polymyarian (arrangement of somatic muscles)
o Cells are numerous and project well into the body cavity
Mode of • Ingestion
transmission • Oro-fecal route
Adult worms • Trilobate/triradiate lips
o 1 dorsal and 2 (ventrolateral)
• Adults possess structures to protect themselves
o PI-3 (Pepsin inhibitor 3)
▪ To protect against digestion of stomach (to prevent being digested by stomach)
o Phosphorylcholine
▪ Suppress lymphocyte proliferation
• Females
o Capable of laying 200k eggs
o Have paired reproductive organs in the posterior two-thirds
• Males
o Have two spicules (also used for holding the female during copulation)
o Smooth striated cuticles
o Single, long, tortuous tubule
Types of Eggs Unfertilized Corticated
• Oval-shape
• Structures inside: coarse lecithin granules
• Glycogen layer surrounds the granules
• Mamillary/albuminous coat (corticated)
• Unfertilized decorticated: no mamillary coat
Fertilized Corticated
• More circular
• Lipoidal/vitelline layer: innermost layer
o Impermeable layer
o Encloses the amorphous mass of
protoplasm
• Glycogen layer surrounds lipoidal layer (Unfertilized Corticated) (Unfertilized Decorticated)
• Mamillary coat
• Fertilized decorticated: no mamillary coat
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• Nutritional deficiencies
o Worms compete for absorption of nutrients
o Lactose intolerance
o vitamin a malabsoprtion
Disease • Incubation period: approx. 60-75 days
• Light infections: usually asymptomatic
o Some don’t even know that they have it (can only know through stool exam)
• Luminal parasite
• Heavy infections: appearance of symptoms
o Abdominal pain
o Vomiting
o Growth stunting (attributed to nutritional deficiency)
o Can cause intussusception (part of the intestine folds into the section next to it) or volvulus
(can result in bowel infarction and intestinal perforation)
o Intestinal spasm (leading to intestinal obstruction)
▪ Caused by worms irritating nerve endings in mucosa
• Lots of worms leads to intestinal obstruction
o worms form bolus
o may lead to intestinal perforation
• worms can become erratic
o wander to other parts of the body
o triggered by high fever, stress, other illnesses, drugs
o escape through nostrils
o may be regurgitated and vomited
o inhaled into the trachea
o may invade bile ducts (through ampulla of Vater) and enter the gallbladder or liver
o Biliary Ascariasis: may experience severe colicky abdominal pain (because of movement of
worms to biliary tract)
o Appendicitis
o Pancreatitis
o Abscess (caused by intestinal bacteria being carried to migration sites)
o Acute peritonitis
o Chronic granulomatous peritonitis
• more eggs ingested = heavier infection
• no autoinfection
Diagnosis • specimen for larval stage: sputum
o because of heart-lung migration of larva
• specimen for eggs: stool
• DFS (less sensitive)
• Kato-Katz
o Quantitative diagnosis
o Intensity of helminth infection (eggs per gram, epg of stool)
• Concentration Techniques
Epidemiology • Soil transmitted helminth
• More common in poor countries where sanitation is poor
• Highly affected: school aged children (grades 1-3)
• Eggs are highly resistant (can survive very harsh environment)
Treatment • DOC: Mebendazole
• Other drugs: albendazole
TRICHURIS TRICHIURA
Life Cycle
Ingestion of • Go to stomach then to small intestine
embryonated egg • Small intestine: where larva emerge
Larva will crawl towards • Becomes adult in the large intestine
the colon • Attached parasites (heads are
embedded in the large intestine)
Parasites reproduce • Unembryonated egg seen in the stool
sample
Unembryonated egg • Maturation time: 10-14 days
goes to loamy soil
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N. Villanueva
Trichuris trichiura
• Common name: Whipworm
• Aka Trichocephalus trichiurius
• Final host: man
• Habitat: large intestine/colon
• Infective stage: embryonated egg
• Soil-transmitted helminth
• Female produces 7k-10k eggs
• No heart-lung migration
• Holomyarian (based on arrangement of somatic muscles in cross-section)
o cells are small, numerous, and closely packed in a narrow zone
Mode of Transmission Ingestion of embryonated egg
Morphology of adult worm • Whip: anterior portion is attenuated/tapered
o Where you can find the esophagus (has a string of beads appearance)
• Posterior portion: robust/thick
• One spicule: retractable/retractile
o Appearance: lanceolate/sword-like
• Anterior portion are embedded in the large intestine
o Pin fashion manner/pin cushion manner
• Male: has a retractile sheath
Morphology of egg • Lemon-shaped
• Barrel-shaped
• Football shaped
• Japanese lantern appearance
• Yellowish outer shell and transparent inner shell
• Unembryonated once released (needs to embryonate in soil)
• Bipolar mucus plugs: where larva goes out
(unstained, no color)
• Shell is nonstriated
• Color: bile-stained (dark yellow/brownish)
• Larva in egg: L1
*Trichuris eggs in soil more susceptible to desiccation
Pathology • Embedded in large intestine
o Secrete TT47 (promote pore formation)
o Cause petechial hemorrhages (may predispose to amebic dysentery, ulcers provide a suitable
site for E. histolytica)
o Mucosa becomes hyperemic (excess of blood) and edematous
▪ Enterorrhagia or intestinal bleeding is common
Disease • Patients usually asymptomatic
• Heavy infections: symptomatic (5000 eggs per gram of feces)
o Diarrhea
o Trichuris dysentery syndrome
▪ Manifested by chronic dysentery and rectal prolapse
o Blood in stool (because parasites are embedded, promote blood loss)
o Inflammation
▪ Prolonged/chronic inflammation leads to anemia
o IDA
o Rectal prolapse (cinnamon roll appearance)
▪ Parasite goes to rectosigmoid area and causes inflammation
▪ Lower portion of colon becomes turned inside out and protrudes outside the body
▪ Rectum loses its internal support due to the worms burying their heads in the intestinal
lining (loosens epithelium and weakens muscles)
o Weight loss
o Appendicitis
o Hypoalbuminemia: low albumin levels in blood because of blood loss
o Appendicitis and granuloma formation
o In children: poor appetite, wasting, stunting, and recued intellectual and cognitive
development
• Due to lumen of appendix being filled with worms
Diagnosis • DFS
• Kato-Katz
• Concentration techniques (FECT)
• FLOTAC technique
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N. Villanueva
Epidemiology • STH
• More common in tropical areas
• Common among children
Treatment • DOC: Mebendazole
• Albendazole, albendazole with ivermectin
ENTEROBIUS VERMICULARIS
Life Cycle
Ingestion of • Go to stomach then small intestine
embryonated egg • Small intestine: where larva hatches
Larva goes to colon • Where it becomes adults
Reproduction in • Female worm migrates to perianal area at night
large intestine o Perianal area for the oxygen requirement of
the eggs
• After laying eggs, female dies
• After copulation, male dies
After 4-6 hours, • Eggs can fall onto the bedsheet
embryonated o Egg goes to air, eggs can be inhaled
• Autoinfection: scratch butt, eggs goes to hand and can
go to mouth
• Retroinfection
Enterobius vermicularis
• Common name: Pinworm, Seatworm, Society worm, “Tiwa”
• AKA Oxyuris vermicularis
• Most common helminth infection in the world
• Final host: man
• Habitat: large intestine
• Infective stage: embryonated egg
• not a soil transmitted helminth
• Meromyarian (arrangement of somatic muscle)
o there are two to five cells per dorsal or ventral half
Mode of transmission • ingestion of eggs (oral)
• air-borne
• retroinfection: eggs hatch in perianal area, larva crawl back to large intestine
• autoinfection
Morphology of adult • anterior portion: wing-like structure (cephalic alae)
worm o chemoreceptor
o employed during migration of the worm (not fully understood)
o other references: cuticular alars
• esophagus: bulb/flask shaped
• male: one spicule
• larva: tadpole-like
*rhabditiform larva has no cuticular expansion on the anterior end
Morphology of eggs • d-shaped
• lopsided eggs
• football shaped with a flattened side
• embryonation after 4-6 hours
• 2 layers
o Albuminous layer (triple covering, for mechanical
protection)
o Lipoidal layer (for chemical protection)
• Adult female lays the eggs in the perianal area (around
11,105 eggs per day)
• Resistant to disinfectants but succumb to dehydration in dry air
o Moist conditions: can last up to 13 days
• larva in egg: L1
Pathology Enterobiasis or Oxyuriasis
• nocturnal pruritus ani (butt is itchy at night, because that’s the time the female lays the eggs)
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• insomnia (due to being awake at night due to itchiness)
• secondary bacterial infection (due to wounds)
• loss of appetite
• abdominal pain
• weight loss
• irritability
• grinding of teeth
• vulvovaginitis: eggs may go to vagina in females (because anal area is near to the vagina)
• mild catarrhal inflammation of intestinal mucosa (due to attachment of worms)
• appendicitis, endometritis, salpingitis, peritonitis, ectopic deposition of eggs may also occur
• innocuous: not harmful, rarely produces any serious lesions
*Familial disease, spreads easily, everyone can be infected (everyone in the family must be treated)
Diagnosis • Scotch tape swab
• Cellulose acetate swab
• Cellophane anal swab
• NIH swab
• Swellengrebel technique: instead of scotch tape, make use of a pestle with petrolatum or
petroleum jelly
o rolled in the area
• Graham’s technique: use of a slide
• Petrolatum
*at least 7 negative swabs before reporting as negative (because eggs are intermittently shed
Epidemiology • Most common helminth infection in the world
• Cosmopolitan: more common among females (locally)
• Cannot be controlled by sanitary disposal of waste
Treatment • DOC: Mebendazole
• Albendazole
• Pyrantel pamoate (secondary drug of choice)
HOOKWORMS
Life Cycle
Adult worm infects via • Worm prefers sandy soil
skin penetration (if • Goes to the blood vessels from the skin
people walk bare-
footed)
From blood vessels, • After, they are swallowed back into the small
worms undergo heart- intestine
lung migration
In the small intestine, • Diagnostic stage: unembryonated egg (found in
they become adults stool)
(become attached to o Rare to recover larva because they are
the small intestine) attached to the small intestine
• 1-2 days for egg to embryonate
• egg is thin-shelled
o hyaline (clear)
o with developing blastomeres (2-8)
o blastomeres have a morula ball formation
Embryonated egg Rhabditiform larva
become the • L1 larva
rhabditiform larva • Feeding stage (open-mouth)
• Buccal capsule (mouth) is longer
• Small genital primordium
• Short and stout
Rhabditiform larva Filariform larva
develops into the • L3 larva
filariform larva • non-feeding stage (closed mouth)
(usually after 7-10 • posterior portion
days, especially with o tail: pointed
unfavorable o membrane is sheathed
conditions) • cannot identify species based on larval
stages
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N. Villanueva
Human Hookworms
• Final host: man
• Infective stage: Filariform (L3) stage
• Habitat: small intestine
• Mode of transmission: skin penetration
o Ancylostoma: can be through oral (eating vegetables with larvae)
• Eggs are indistinguishable
• Undergoes heart-lung migration
• Identification of species: dental pattern/buccal cavity
• Necator americanus: more common in the Philippines
• Males would have the copulatory bursa (used during copulation)
• They are attached parasites
o attached to lining of small intestine (that’s why they are called hookworms)
• cellular immune response mediated by eosinophils, mast cells, and Th2 cells
o but still no clear evidence that host develops perpetual immunity to hookworm infection
o polyvalent IgE antibodies: suggested to provide some protective roles
• Meromyarian type of somatic muscle (2-5 cells arranged per dorsal or ventral half)
Ancylostoma duodenale Necator americanus
Common name Old World Hookworm (Europe) • New World Hookworm
• American Murderer
Adults Larger Smaller (cylindrical, fusiform, grayish-white)
Curvature C shape S shape
Color Pinkish or creamy gray color Grayish yellow with reddish undertone
Life Span 5-7 years 4-20 years
Dental pattern/ buccal 2 pairs of ventral teeth (inner pair is smaller) No teeth, contains semilunar cutting plates
cavity
Buccal spears (of • Inconspicuous • Conspicuous and parallel
filariform larva) • Transverse striations on the sheath in • Conspicuous transverse striations present
the tail region on the sheath in the tail region
Copulatory bursa • Bell-shaped • Bipartite dorsal rays
(campanate
appearance)
• Tripartite dorsal
rays
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N. Villanueva
• Steatorrhea
*hookworm infection usually chronic (patients rarely show acute symptoms)
*Hypoalbuminemia – low level of albumin due to combined loss of blood, lymph, and protein (other
symptoms: exertional dyspnea, weakness, dizziness, lassitude, rapid pulse, edema, and albuminuria)
Diagnosis • DFS (only when infection is heavy)
• Kato-Katz (not recommended because hookworm eggs are thin-shelled)
o Shell will disintegrate easily (will give a false negative result)
• Concentration Techniques (Zinc sulfate centrifugal flotation, FECT)
o Use greater amount of stool (contribute to increase in sensitivity)
o FLOTAC: higher sensitivity for the diagnosis of STH
• Harada Mori Technique
o Materials: filter paper for fecal material and test tube
o Moisture maintained by adding boiled or distilled water
o Incubation under suitable conditions will favor hatching of ova and/or development of
larvae
o Stool samples should not be refrigerated
o Filter paper strip should be handled carefully
o Eggs in stool samples hatch and migrate towards the water
• Coproculture
• Baermann technique
• Molecular methods (PCR, ELISA)
Epidemiology • Both species distributed widely
• Males more prone to infection (farmers)
• More blood loss in females (menstruation)
• Pregnant women and children at high risk
• Eggs embryonate best in damp sandy soil and at a temp of 24-32 degrees Celsius
• Other modes of transmission for Old World Hookworm
o Transmammary transmission (via breast milk)
Treatment • Albendazole (DOC)
• Mebendazole
• Pyrantel Pamoate
Animal Hookworms
• Ancylostoma caninum Pairs of Teeth
o Common name: Dog hookworm (final host: dog) N. a. – 0
o 3 pairs of teeth (largest mouth among hookworms) A. b. – 2
• Ancylostoma braziliense A. c. – 3
o Common name: Cat hookworm (final host: cat) A. d. – 2
o 2 pairs of teeth
• Humans only accidental hosts Size
C> D> A> B
• MOT: skin penetration of L3 larva
C = caninum
o Only penetrate underneath the skin
D = duodenale
o Not capable of maturation in humans
A = americanus
o Creeping eruption (Cutaneous Larva Migrans)
B = braziliense
• Patients infected: have elevated eosinophils
Trichostrongylus
• Related to hookworms
• Final host: herbivores (cow, sheep)
• Similar to hookworms, but have no heart lung
migration
• Eggs: similar to hookworm
o Symmetrical, thin-shelled, pointed ends
o Size: larger than hookworm egg
o Trichostrongylus: larger, elongated, ends are
pointed
o Hookworms: smaller, more rounded ends
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N. Villanueva
STRONGYLOIDES STERCORALIS
Life Cycle
Facultative: free- • Free-living in the environment (top portion
living and of the soil)
parasitic phase • Male and female copulate
• Eggs embryonate in the soil (loamy, but not
really specified what kind of soil)
• L1 found in the soil
Parasitic phase • L1 becomes L3
begins when • Females only ones to become parasitic
conditions o capable of self-fertilization (parthenogenesis)
become • L3 penetrates skin of humans
unfavorable • Heart-lung migration occurs
Larva goes back • L3 becomes adult worm
to small intestine • Eggs embryonate and hatch in the small intestine
of humans o Eggs have a Chinese lantern appearance
(similar to hook worms)
• L1 in stool, then goes to soil
• L1 becomes adult in the free-living phase
Possibility of • L1 larva in the small intestine can already
autoinfection transform into the L3 larva
• Trigger autoinfection
• Internal multiplication happens (increased
number of worms in the small intestine)
o Lead to hyperinfection
• Infections can last to many years
Strongyloides stercoralis
• Common name: Thread Worm
• Smallest nematode of man
• STH
• Facultative parasite (free-living and parasitic phase)
• Habitat: small intestine
• Infective stage: filariform larva
• Heart-lung migration occurs
• Eggs are rare in the stool sample
o Because they become larva immediately
o Stool sample contains larva = most likely Strongyloides stercoralis
o Clear thin shell (similar to hookworms)
• Parasitic males have not yet been identified
• Parasitic females: Parthenogenetic
• Free-living females smaller than parasitic female
o Has a muscular double-bulbed
esophagus
o Intestine: straight cylindrical tube
• Free-living male smaller than female
o Has a ventrally curved tail, two
copulatory spicules, a gubernaculum,
but no caudal alae
• Strongyloides fulleborni
o Affects small children in Central Africa
o Can also infect monkeys, racoons, etc.
o Swollen belly sickness
• Zoonotic Strongyloides
o Swimmer’s itch and swamp itch
o Ex: S. myopotami, S. procyonis
(raccons)
Mode of transmission Larval penetration
Rhabditiform larva • short buccal capsule (has four indistinct lips)
• big/prominent genital primordium
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N. Villanueva
• long and slender
• elongated esophagus with a pyriform posterior bulb
• slightly smaller and less attenuated posteriorly compared to hookworms
Filariform larva • posterior: forked/notched tail
o has a distinct cleft at the tip of the tail
• unsheathed
• female: colorless, semi-transparent, finely striated cuticle
Pathology • larval invasion of skin produces erythema and pruritic elevated hemorrhagic papules
• larval migration phase: causes lobar pneumonia with hemorrhage
• intestinal phase
o Vietnam Diarrhea (because of war veterans)
o Cochin China Diarrhea (based on the place)
o Severe and intermittent diarrhea
• Honeyform appearance of intestinal mucosa
o Eggs inside the small intestine
• Hyperinfection and dissemination
• Larva currens
o Allergic reaction attributed to penetrating larva
• Heart-lung migration causes pneumonitis (has no specific name)
• Infection can persist for many years (autoinfection)
*chronic strongyloidiasis often asymptomatic
Diagnosis • FECT: larva
• Baermann Technique (for high volume of specimen, and soil specimens, preference for
diagnosis depends on condition)
• Beale’s String Test
• Harada Mori
• Enterotest
Epidemiology • Less common as compared to hookworms
o Least common among STH
• Target elders, AIDS patients, and immunocompromised
o Young: get infection
o Recovered, but their immune status is weak so parasite still underwent reproduction in
small intestine, lead to autoinfection (can happen many years after initial infection)
• Most cases asymptomatic
• Disseminated and hyperinfection more common among immunocompromised
Treatment • Ivermectin with albendazole
CAPILLARIA PHILIPPINENSIS
Life Cycle
• Still not sure (still has conflicting results)
• Made from animal experiments
Infection from • Small intestine: larva emerge from fish
raw/undercooked fish • larva also becomes adults in the small
with the larva intestine
o First generation: females
are always larviparous
▪ Lays larva and larva
becomes 2nd gen
o Second generation: females
are oviparous
Diagnostic stage: • For life cycle to continue, must find its
unembryonated eggs way to freshwater
o Will embryonate in the
freshwater after a few days
Fish ingest • Larva emerge
embryonated eggs • Humans get infected once they eat the
fish
Birds eat fish • Migratory birds get infected
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N. Villanueva
Capillaria philippinensis
• Common name: Pudoc Worm, Mystery Disease
o First reported in the Philippines
o Pudoc: barrio in Ilocos, people had a severe outbreak of diarrhea
o Discovered by Nelia Salazar (a member of the team who studied the parasite)
• From superfamily Trichinelloidea
• Final host: humans, other vertebrates
• Natural host: migratory birds
o If no humans available to be final hosts
• Intermediate hosts: freshwater or Brackish fish (Glass Fish)
o Ex: Bagsit, Ipon, Birot, Bagsang
• Foodborne parasite
o Raw or undercooked fish
• Habitat: small intestine
• Infective stage: Larva (don’t indicate na the stage)
o Tissue or muscles of fish
• Deadly if not treated
o Causes severe diarrhea
o Autoinfection, hyperinfection, and superinfection because of the larviparous female
• Other species
o C. hepatica – affect the liver (hepatic capillariasis)
o C. aerophila – affect the lungs (pulmonary capillariasis)
o C. plica – urinary capillariasis
Mode of Transmission Ingestion of undercooked fish containing larva
Adult Worms Typical female
• Has 8-10 eggs in utero arranged in a single row
• oviparous
Atypical female
• Has 40-45 eggs in utero arranged in 2 to 3 rows
• Ovoviviparous
• Larviparous in other references
Male worm
• Spicule: chitinous, sheathed
Both Males and Females
• Stichocytes (secretory cells in the anterior portion)
• near esophagus
• many stichocytes = stichosome
Eggs Guitar-shaped (typical egg by typical female)
• Also peanut shaped
• Bipolar mucus plugs: flattened
• Striated shell
• Usually mistaken as Trichuris
o Smaller than Trichuris
• Color: pale brown
Atypical egg
• Already embryonated
• No mucus plugs
Pathology • Pudoc Disease, Mystery Disease (thought people were cursed), intestinal capillariasis
• Severe diarrhea
• Abdominal pain
• Borborygmus: gurgling of the stomach
• Death may occur if left untreated
• Protein losing enteropathy
• Electrolyte loss
• Autoinfection can occur
Epidemiology • Highly pathogenic (all infected persons will eventually develop the disease)
• Endemic areas: Ilocos region, La Union, Pangasinan, Zambales, Cagayan, Isabela,
Compostella Valley, Zamboanga del Norte
o Also in Mindanao (because of migratory birds)
Treatment • DOC: Mebendazole
• Albendazole
• Electrolyte replacement and high protein diet
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ANISAKIASIS
Life Cycle
Parasite lives in stomach of • Eggs found in the stool of
marine mammals/dolphins dolphins
(adult worms are embedded • Eggs embryonate in water (L2
in the gastric wall) larva)
Eggs hatch • When it comes out, becomes L3
larva (infective stage to final
host and paratenic host)
Cyclops can eat the L3 larva • Then get eaten by the paratenic
hosts
• L3 more concentrated in the fish
viscera, but can also be found
in their muscles
• Dolphins eat the fish
• Dolphins can also eat the
Cyclops immediately
Man eat • Larva in the small intestine
fish/squid/crustaceans
Anisakiasis
• Anisakis simplex and Pseudoterranova decipiens
• Other related species: Contracaecum sp., Hysterothylacium sp.
• Common name: Cod Worm, Herring Worm
• Not yet documented in the Philippines
• Nematode parasites of whales, dolphins, porpoises, walruses, seals, sea lions, and other deep-marine mammals
• Have elongated vermiform bodies without segmentation
• Final host: marine mammals (dolphins, whales)
• Paratenic host: fish (small) and squid
o Harbor larva, no development
• Accidental host: humans (L3 do not develop into adults)
• Intermediate host: Microcrustaceans (Cyclops)
• Infective stage (to humans): L3 larva (to both humans and animals)
Mode of transmission Ingestion of undercooked or raw seafood containing larva (saltwater fish, squid, rare in Crustaceans)
L3 Larva Anisakis simplex
• Milky white color, 19-36 mm in length
• Has a long stomach
• Has a blunt tail with a mucron (attachment organelle)
• This is called the Type I larvae
• Other species of Anisakis: L3 have shorter stomachs and blunt tails, are called Type II larvae
Pseudoterranova decipiens
• Yellowish brown in color, 25-50 mm in length
Pathology Anisakiasis or Anisakidosis
• Aka Herring’s Disease, Gastrointestinal Anisakiasis
• Occurs within 1-12 hours after ingestion
• Abdominal pain, nausea, vomiting diarrhea
• Irritation of small intestines
• Eosinophilic granulomatous reaction
o larva invade the submucosa of the small intestine
o this results in hemorrhage and inflammation
o larvae may die and detach
o if penetration is deep, causes an eosinophilic granulomatous/allergic reaction
• urticaria (hives), asthma, conjunctivitis, contact dermatitis are observed among workers in fish
and marine product processing factories
• Perforation of intestines reported (in heavy infections)
• You can cough out the parasites (regurgitated)
• Symptoms can be mistaken for peptic ulcer disease, cholecystitis, or even gastroenteritis
• Gastric form mimics appendicitis, Crohn’s disease, intestinal obstruction, or diverticulitis
• Ectopic anisakidosis: larvae found invading the oropharynx, esophagus, and colon
o Tingling throat syndrome: when oropharynx is involved
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Epidemiology • Common in Japan and Netherlands
• Also common in Korea, France, Germany, Italy, Spain, UK, North and South America, Egypt
• Common among coastal populations (because of fish)
• Ingestion of sushi or sashimi or pickled herring
Diagnosis • Gastroscopic Examination (Endoscopy, Gastroscopy)
• Biopsy
• Worms may be coughed off by patients
• Serology: ELISA, Radioallergosorbent Test (RAST)
Treatment • Albendazole
• Surgery or removal of larval stages
TISSUE NEMATODES
ANIMAL ASCARIDS
PARASTRONGYLUS CANTONENSIS
Life Cycle
Rat ingested snail with the • L3 mature in the lungs of rats
L3 larva (can also ingest the • Eggs in stool
vegetables/leaves where the
snail passes through, with
the slime of the snail)
Humans eat snails • Larva will not become adults
• Larva goes to the brain
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Parastrongylus cantonensis
• Aka Angiostrongylus cantonensis
• Formerly Haemostrongylus ratti
• Common name: Rat Lung Worm
• Definitive host: Rats (Rattus rattus)
• Humans are accidental or incidental hosts only
• Intermediate hosts
o Achatina fulica (giant African snail)
o Hemiplecta sagittifera
o Helicostyla macrostoma
o Vaginilus plebeius
o Veronicella altae
• Paratenic hosts: prawn, crab, vegetation
• Cantonensis: in Canton, China
• Infective stage: L3 Larva
• Habitat: lungs of definitive host (two main branches of pulmonary arteries)
• Eggs contain L1 larva (eggs have delicate hyaline shells)
Mode of Transmission • Ingestion of raw or undercooked infected snails
• Ingestion of paratenic hosts
• Drinking contaminated water
• Eating salad where the snail was
Larva • L1: distinct small knob near tip of the tail
• L3: have two well-developed chitinous rods below its buccal cavity (rods have expanded knob-
like tips)
Adult Worms Female
• Barber pole appearance
o Dark: intestine
o White: uterus
Male – has the spicule
*both have well-developed caudal bursa (kidney-shaped and single lobed)
Disease Manifestation Angiostrongyliasis/Parastrongyliasis
• Eosinophilic meningoencephalitis
o Headache, vomiting, neck stiffness, seizures, eosinophilia, neurologic abnormalities
o Inflammation of brain meninges
o Headache: intermittent occipital or bitemporal headache
o Can cause diplopia (blurring vision)
o Increased eosinophils
• Incubation period: 6-14 days (or 12-47 days)
• Ocular involvement reported (intraocular hemorrhage and retinal detachment)
• Prognosis is good (usually self-limiting)
• Postmortem examination may show leptomeningitis, encephalomalacia, moderate ventricular
dilation
• Dead worms can result in inflammatory reaction and local tissue necrosis
Diagnosis • Difficult
• Travel history and exposure must be noted
• CSF examination: more than 10% of WBCs is composed of eosinophils
o CSF Protein: mild elevation
• Charcot-Leyden crystals may be seen in the meninges
• CT-Scan
• Serology
• PCR
Epidemiology • Described among rats in Canton, China in 1935
• Human infection reported in Taiwan in 1945
• Endemic Areas: Southeast Asia, Pacific Basin
• Ocular Angiostrongylus has been reported
Treatment • No recommended treatment
• Mebendazole
• Thiabendazole
• Albendazole can be given
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TRICHINELLA SPIRALIS
Life Cycle
MOT for Pigs: Pigs develop the parasite
• Domestic life cycle
o Pig eat scrap food with larva
o Pig eats another pig (carnivorism)
o Pig eats rat
o Rat can also transmit it to other rats
• Sylvatic life cycle
o Omnivore and carnivore
o fraction of the pathogen population's
lifespan spent cycling between wild
animals and vectors
People can get the parasite upon accidental • Larva goes to
ingestion of pork with larva or when they are stomach then to the
hunting (also through improper preparation of small intestine
pork, like microwaving) • Adults in the small
intestine
• Reproduce then
produce larva
Larva penetrates mucosa of the small intestine • Goes to the striated
muscle
• Cyst: produced by
nurse cells of the
parasite
Trichinella spiralis
• Common name: Trichina Worm, Muscle Worm, Garbage/Trash Worm
• Nematode found in the tissues
• Final host: pigs and other mammals
• Man only an accidental host
• Habitat: small intestine, striated muscle
• Infective stage: encysted larva (life span of 5-10, or up to 40 years)
• Larval stages usually found in gastrocnemius, deltoid
• Other distinct species
o T. nativa: occurs in arctic and subarctic zones
▪ high pathogenicity
▪ high resistance to freezing
o T. nelsoni: occurs in tropical Africa
o T. britovi: occurs in temperate Paleoarctic region, very low
pathogenicity, 2nd most common
o T. pseudospiralis: cosmopolitan, does not encyst, infectious to birds
o Others: T. murrelli and T. papuae
• Host serves as both the final and intermediate host (harbors both adult and larval stages)
• Larva: has a spear-like, burrowing anterior tip (what it uses to burrow into the sub-epithelium of the villi, where they undergo
four molts)
*still has larval stages, just not shown
Mode of Transmission Ingestion of undercooked or raw pork (other meat) infected with larva
Adult worms Male
• Has conical papillae (for copulation, located in the posterior)
o For holding the female during copulation
• Has a single testis
Female
• Has the larva inside
• Has a single ovary
• larviparous
Disease Manifestation Trichinosis or Trichinellosis
and Pathology • Intestinal/Enteric phase: minor, usually non-specific gastroenteritis
• Muscle invasion (Invasive Phase)
o Pathology associated to the larva
o Fever and eosinophilia
o Induces greatest increase in eosinophils
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o High levels of IgE
o Muscle pain
o Myocarditis (most severe symptom): can cause pericardial pain, tachycardia, and
ECG abnormalities
o Some may experience CNS involvement
▪ Psychosis, meningoencephalitis
• Convalescent phase: fever, weakness, pain, and other symptoms start to abate
• Usually good prognosis (full recovery expected)
Diagnosis • Biopsy of Muscle (gold standard, or definitive test)
o Shows presence of larva
o Can use deltoid, gastrocnemius, pectoral, gluteus
• Biochemical tests
• CBC: show increased eosinophils
• Blood Chemistry: shows increase creatine kinase and lactate dehydrogenase
• Very high levels of eosinophils
• ELISA (detection of antibodies)
• Bentonite Flocculation Test
o Serologic test using the serum sample
o Detect antibodies against Trichinella
o Reagent: Trichinella antigen attached to Bentonite (carrier particle)
o Positive result: flocculation (reaction that is in between agglutination and
precipitation)
o Bentonite: clay
• Bachman Intradermal Test: use of 1:10,000 dilution of larval antigen
o Patient injected with Trichinella antigen in the skin
o Positive result: wheal and flare reaction
▪ Indicates previous exposure to the parasite
o Also a type of xenodiagnosis
• Beck’s Xenodiagnosis
o Uses albino rats
o Rat eats muscle, kill the rat after around 14 days
o Then you can find the female worm in the small intestine
Epidemiology • Life cycle maintained in the environment by carnivores
• reservoir hosts: boars and bears
• Humans usually infected by ingestion of raw or undercooked pork
Treatment • Mebendazole (Intestinal Phase)
• Steroids and Mebendazole (for severe acute infections, muscles)
Prevention • Health education
• Thoroughly cook meat (77 degrees Celsius)
• Freeze meat (-15C for 20 days or -30C for 6 days)
• Cannot be prevented via handwashing
DRACUNCULUS MEDINENSIS
Life Cycle
Humans ingest • Copepods goes to stomach
water contaminated • L3 larva penetrates the
with the copepods stomach
or L3
Larva goes to the • Develop into adults
subcutaneous • Diagnostic: find only 1 or 2
tissue pairs of male and female
worms
After 1 year of • Migrates to the surface of the
maturation, female skin
is ready to release • Forms a blister (where female
the larva emerges to release the L1)
• Release of larva triggered if
person immerses feet in the
water
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Dracunculus medinensis
• Common name: Guinea Worm, Fiery Serpent, Medina Worm, Dragon Worm
• Largest nematode infecting man (female around 1 meter in length)
• Parasite of antiquity (old parasite)
• Final host: humans
• Intermediate host: Copepods (Cyclops) found in freshwater
• Infective stage to FH: L3
• Infective stage to IH: L1
• Habitat: subcutaneous tissue
• Primarily found in Africa
Mode of Transmission Ingestion/Drinking of contaminated water with the copepods or L3
Disease Manifestation Guinea Worm Disease; Dracunculiasis
• Formation of blister
• Urticaria
• Vomiting
• Diarrhea
• Asthma attacks
• Symptoms disappear once ulcers appear
• Discharge of the worm
Epidemiology • Water sources can be breeding grounds for copepods
o Ex: stepwells of India, open ponds, Open Cisterns
• Important parasite in middle east, Central India Pakistan, Africa
o Can also be found in Asian countries
• Disease is nearing its eradication
Diagnosis • Appearance of cutaneous lesion and worms
• X-ray: visualization of calcified worms
Treatment • Removal of worms using a stick
o Don’t break the worm
▪ will trigger an allergic reaction
▪ can also cause calcification of the parasite
▪ will need surgery to get the worm out
• can immerse foot in water to make the larva go out
• DOC: Metronidazole
FILARIAL WORMS
Life Cycle
Mosquitoes suck human blood • Drops of blood drop on skin
• Blood contains the larva (penetrates the skin through the bite wound)
• Larva is not injected
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L3 larva goes to lymphatic system • Develops into adult male and female
• Reproduce to produce microfilaria
Microfilaria released to bloodstream • Passes through the lungs (blood vessels of lungs)
• Goes to the peripheral blood
• Diagnostic stage
Mosquito bites human • Ingests microfilaria
• Microfilaria goes to mosquito’s stomach
• Sheath sheds
Unsheathed microfilaria migrates to the • Microfilaria becomes L1 then L3
thorax of the mosquito • L3 migrates to proboscis
Filarial Worms
• Malayan Filariasis: seen in Palawan, Eastern Samar, Agusan del Sur, Sulu
• Disease seen more in adults than children
• Males more affected
• Final host: man
• Intermediate host: mosquito
• W. bancrofti: prefer localization in scrotal lymphatics
• B. malayi: less severe
• Infective stage to FH: L3 filiform larva (not filariform, describes the
appearance)
• Infective stage to IH: microfilaria (sheathed)
• Diagnostic stage: microfilaria
• ideal breeding sites for mosquitoes:
• Colocasia esculenta
• Musa textilis
o Water lilies
• Those constantly exposed: have less severe symptoms
o Immune system is primed/exposed
o People not exposed: when they go from a non-endemic to an endemic area, they may experience more severe
symptoms (called the Expatriate Syndrome)
Wuchereria bancrofti Brugia malayi
Common name Bancroft’s Filarial Worm Malayan Filarial Worm
Intermediate host Mosquitoes • Mansonia bonneae (freshwater)
• Aedes poecilus (transmit in urban areas) • Mansonia uniformis (rice paddies)
• Anopheles flavirostris • Anopheles
• Culex (primarily transmit in agricultural
areas)
Life Span 10 years
Movement Graceful, smooth movement Kinky (angular) movement
Adult Worms • Sheathed • Sheathed
• Non-overlapping/organized body nuclei • Overlapping/non-organized body nuclei
• Unstained with Giemsa • Stains pink with Giemsa
• No posterior/terminal nuclei • Contains posterior/terminal nuclei
• Shorter than Wuchereria
Periodicity Nocturnal Subperiodic (12pm-8pm)
*microfilaria released during the day
Cephalic Space Length = breadth (width) Length = 2 x breadth
Habitat Lymphatics (in adults)
Mode of Transmission Active larval penetration
Disease Manifestation Lymphatic Filariasis
• To get the infection, they are constantly bitten by the mosquito
• Asymptomatic Phase: thousands to millions of microfilaria in the blood and adult worms in the
lymphatics
o Seen in “Endemic Normals” (they have in their blood the parasite antigen instead of
the microfilariae)
o Patients appear healthy and no clinical manifestations seen
o Microfilaria come from lymphatic vessels (from the adult worms there)
o Symptoms are nonspecific
• Early Manifestation: Fever, lymphadenitis, welling, redness of arms and legs, vomiting,
headache
o Lymphadenitis: inflammation of lymphatic vessels, triggered by the adult worms
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o Main pathology attributed to adult worms
• ADLA (Acute Dermatolymphangioadenitis)
o Inflammation of lymph
o Adenolymphangitis
o Swelling or inflammation of lymphatic vessels
o Most common manifestation
o This is of bacterial etiology (most frequently associated with Group A Streptococcus)
• Constant irritation and inflammation triggers chronic phase after many years
• Chronic Phase: Elephantiasis, Hydrocoele, Chylocoele, Lymphocoele, Chyluria
o 10 years or more after the initial infection
o Elephantiasis: enlargement of different parts of the body
o Parasites might be dead already (life span of around 10 years)
▪ Parasites calcify and cause obstruction and inflammation (this triggers the
Elephantiasis, Hydrocele, Chylocele, Chyluria)
• Accumulation of lymphatic fluid
• Chyluria: fluid find its way to the urinary system (discharge of urine
is milky)
• Wolbachia: also attributed to the pathology, type of bacteria
o Gram-negative, type of Rickettsia
o Found in the insect vector and inside the filarial worms
o If worms die, this will also be excreted and also triggers inflammation
o Promotes inflammation in Filariasis
• Tropical Pulmonary Eosinophilia – attributed to microfilaria
o Microfilaria in tissues (passes through the lungs and tissue)
o Paroxysmal nocturnal cough
▪ Respiratory symptoms, lung function impaired
▪ Coughing during the night coinciding with release of microfilaria
o Hypereosinophilia
o Type of Occult filariasis (hidden filariasis, because none in blood)
• Wuchereria: more severe deformity
o Enlargement happens below the waist
o Thighs, males: scrotum
• 2nd leading causes of permanent disability
o Enlargement is irreversible
• Brugia: can selectively induce CD4+ lymphocyte apoptosis (can contrinute to immune
unresponsiveness to filariasis)
Diagnosis • Best to diagnose early (when acute and asymptomatic)
• Thick smears (Giemsa) – look for microfilaria
o W. bancrofti: collect blood from 10 pm to 2 am (Nocturnal Periodicity)
o B. malayi: subperiodic periodicity
• Diethylcarbamazine Provocative Test
o Orally
o Done especially if difficult to collect sample during the night
o 3 mg/weight kilogram of patient
o Triggers release of microfilaria
o With this, do not need to wait for night for microfilaria
• Concentration Techniques
o Knott’s Concentration Technique
▪ 9 ml of 2% formalin added to 1 ml of whole blood
▪ Formalin added to lyse RBCs (better visualize microfilaria)
▪ Centrifuge
▪ Positive result: sediment
▪ Sediment stained with Giemsa
o Membrane Filtration Method (Nucleopore filter)
▪ Pore size is smaller than the microfilaria
▪ RBCs pass through the filter
▪ Residue on filter paper = microfilaria
• Serology
o Detection of circulating filarial antigen (CFA)
o Rapid diagnostic test
▪ Brugia Rapid (using Ag BmR1, for Brugia)
▪ Using WbSXP-1 (for Wuchereria)
• Use of urine samples
• Molecular methods
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• Ultrasonography (useful for elephantiasis)
Epidemiology • Bancroftian Filariasis: more widespread, affects India, SEA, Pacific Islands, Africa, and South
and Central America
• Malayan Filariasis – affects SEA
• Common in Bicol (because of abaca, where the mosquitoes breed)
o Most affected: farmers working in abaca plantations
• Disease among adults (elephantiasis)
o Because they got the infection when they were children, manifested when they were
adults already
Treatment • DOC: Diethylcarbamazine (DEC)
• Ivermectin
• Albendazole
• doxycycline
• For elephantiasis, doctor aspirates fluid
o Can lead to bacterial infections
Prevention • Insect repellants
• Vector control
• Mass drug administration (MDA)
Brugia timori
• Similar to bancroftian filarisis in clinical presentation
• Microfilaria
o Longer than B. malayi
o Cephalic space = 3:1
o Sheath unstained by Giemsa
o Periodicity: nocturnal
o Vector: Anopheles barbirostris
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LOA LOA
Loa loa
• common name: African Eye Worm
Vector • Chrysops, Mango fly, Tabanid fly
Disease Manifestation Loaiasis
• worms able to migrate through tissues and even through conjunctival tissue across the
eyeball
o Edema of Conjunctivitis and eyelids
o Calabar swellings: localized subcutaneous edema (also called fugitive
swelling)
▪ Allergic reaction indicative of Loa loa
• Cause blindness
• Progressive keratitis
Diagnosis • Presence of calabar swellings (allergic reaction)
• Appearance of worm in conjunctiva
• Detection of microfilaria
• Skin test
Periodicity Diurnal (during the day)
Epidemiology Sudan, Congo, West Africa
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TREMATODES
SCHISTOSOMA SPP.
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o Factors that influence infection of snails: age of snails and miracidium, number
of miracidium per snail, length of contact time, water flow, turbulence
o Ciliated surface disappears once penetration is complete
• Miracidium becomes sporocyst (no redia stage)
o Miracidium develops into mother sporocysts after a few days
o After 96 hours after penetration, becomes an elongated sac filled with germinal
cells
o On 8th day, germ cells bud off epithelial lining and develop into daughter
sporocysts
o Daughter sporocysts migrate to liver through the loose connective tissue
• Sporocyst reproduce asexually and become free swimming cercaria (after 60 to 70
days)
o Limiting factor of number of cercaria produced: size of snail host (S. mansoni
and S. haematobium have bigger snail hosts, have more cercaria, Biomphalaria
biggest intermediate host)
o Singly infected snails: shed 230 cercariae
o Snails with multiple infections: shed 280 cercariae
o Cercaria release usually during night
• Cercaria released from 1st intermediate host
Cercaria swims to patient • Chemotaxis: swims toward light (detects antigens from human host)
• Penetration stimulated by skin lipids
• Dimethylate and niclosamide repel cercaria when applied to skin, but impractical due to
need to frequent reapplication
Schistosoma spp.
• Male: shorter and thick
• No pharynx
• Female: longer and thin/slender
• Contains the uterus and ovaries
• Found in copula (in copulation)
• Entrenched together (most romantic)
• Female inside the gynecophoric canal on the male
• Final host: man
o But has a wide range of definitive hosts (domestic mammals such as dogs, pigs, cats, carabaos, and cows
o Susceptibility to infection can vary (some may be permissive and become infected over an extended period of time
and some may be non-permissive wherein schistosomes are stunted or mature but die prematurely)
• Intermediate host: snails
• Infective stage: fork-tailed cercaria
• Habitat: mesenteric veins, blood
• MOT: skin penetration
• japonicum: found in the Philippines, Japan, China, and other Asian countries
• mansoni and intercalatum: Africa
• haematobium: Africa and Middle East
• mekongki: Mekong river basin in Cambodia, Myanmar, Asia
• injection of irradiated cercariae of Chinese strain confers resistance against
homologous strain (but not against Philippine strain)
• mouse pathogenicity of Chinese strain is less than Philippine strain
• most studies of biology of S. japonicum have been done on the Leyte strain
• eggs in multicellular stage when released
o immature eggs passed in feces no longer mature in soil and are not viable
o mature eggs in feces can survive and still hatch up to a week if desiccation is slow
• male: testes arranged in one row above ventral sucker
• female: single pyramidal ovary located in midline
• worms ingest red blood cells and possess a protease (hemoglobinase) that breaks down globulin and hemoglobin
o also utilize glucose at a rapid rate
o absorb nutrients through the body wall
• main pathology due to the egg
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S. japonicum S. mansoni S. haematobium
Common name Oriental Blood Fluke Manson’s Blood Fluke Vesical Blood Fluke
Habitat Superior mesenteric vein of small Inferior mesenteric vein of the Vesical, Prostatic, Uterine
intestine colon Plexuses of the Venous
Circulation
Intermediate Host Oncomelania hupensis quadrasi Biomphalaria Bulinus
Adult stages Largest Smallest
Location of ovary Middle Anterior ½ Posterior ½
Egg Production Greatest (3000 eggs per worm pair 190-300 eggs/day 30 eggs/day
per day)
Integumentary None Prominent/Grossly tuberculated Fine
Tuberculations
Number of Testes 6-8 8-9 (arranged in a zigzag pattern) 4-5
Eggs Oval with recurved hook or knob, Elliptical with lateral spine Elliptical with terminal spine
smallest
• ovoid, round, pear-shaped
• pale yellow
• thin-shelled (with residual
tissue or red cells adherent
to it)
Reservoir Host Humans and other mammals (dogs, Humans, non-human primates Humans
pigs, cats, carabaos, rodents,
monkeys)
Disease Distribution China, Indonesia, Japan, PH Africa, Madagascar, West Indies, Africa, Middle East, India,
Suriname, Brazil, Venezuela Portugal
Disease (S. Oriental Schistosomiasis, Snail Fever, Schistosomiasis japonica
japonicum) • Initial phase
o When cercaria penetrates the skin
o Schistosome Cercarial Dermatitis
o Trigger allergic reaction and inflammation, redness where parasite enters
o Petechial hemorrhages
o Localized edema and pruritus/itchiness
o Schistosomule goes to blood vessels
• Pulmonary Schistosomiasis – for migrating larval stages, transient period
o Migrates to lungs, causes respiratory manifestations
o Cor pulmonale: dyspnea, cough hemoptysis
▪ Cor pulmonale: abnormal enlargement of right side of heart because of disease
of the lungs or pulmonary blood vessels
▪ Parasite can migrate to heart
o Adult worms, when migrating, can pass through the liver
• Acute phase: Katayama Syndrome/Fever
o Happens 1-3 months after initial phase
o Parasite in the mesenteric veins
o Abrupt onset of fever, chills, muscle pain, headache, hepatomegaly, lymphadenopathy,
dysentery
▪ Dysentery: bloody diarrhea, due to the eggs, eggs pass through blood vessel
linings and go to the intestines
o Fulminating meningoencephalitis with fever, confusion, lethargy, coma
o Acute and intestinal phases are usually overlapping
• Intestinal phase: mucosal inflammation, hyperplasia, ulceration microabscess, blood loss, lower
abdominal pain
o Colonic involvement: during early period of egg deposition, ulcerations cause dysentery or
diarrhea
o Eggs are released to the liver (because blood vessels supplies the liver)
• Chronic infection: Hepatosplenic Schistosomiasis
o Eggs become trapped up by the portal blood flow (abundant eggs in the liver)
▪ Trigger inflammation reaction because eggs are antigenic (triggers immune
system)
o Accumulation of eggs in the portal triads
▪ Eggs cause obstruction
▪ Form granulomas: masses/aggregates of activated macrophages and immune
cells
▪ Causes portal hypertension and hepatosplenomegaly
o granulomatous inflammation
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o granulomas eventually cause fibrosis – Symmer’s Pipe Stem Fibrosis (also called
Fibro-obstructive Hepatic Schistosomiasis)
▪ hallmark/pathognomonic for Schistosomiasis
o Leading to Esophageal Varices: varicose veins in esophagus, can explode and lead to
death
o Leading to Massive Ascites: accumulation of ascitic fluid (stomach enlarges)
o Tumors and increased intracranial pressure
• Aberrant migration may obstruct circulation of brain and spinal cord (can cause seizures,
paresthesias, transient ischemic attacks, and strokes)
• Cerebral Schistosomiasis (motor or sensory disturbances)
o Highly associated with S. japonicum, high propensity, lower risk in other species
• Primary lesion is a granulomatous hypersensitivity reaction around a single egg or egg cluster
• Link to occurrence to colon cancer and liver cancer
o Prolonged inflammation = can cause cancer
Disease (S. mansoni) Intestinal Bilharziasis
• Cercarial Dermatitis
• Acute Schistosomiasis = Katayama like fever
• Association with kidneys
o Hepatosplenic Schistosomiasis (similar to S. japonicum)
o Nephrotic syndrome (affects glomerulus, due to circulating immune complex that
triggered inflammation in the kidneys)
• Spinal cord schistosomiasis
• Manifestations almost the same with S. japonicum but less severe
Disease (S. Urinary Bilharziasis, Egyptian Hematuria
haematobium) • Egyptian – mostly seen in the Middle East
• Light infections usually asymptomatic
• Symptoms found in heavy infections
• Early signs and symptoms: dysuria and hematuria
o Not much diarrhea, because parasite found in blood vessels supplying the bladder,
symptoms are more on the urinary side
• Vesical mucosa: yellow sandy patches
• Granuloma formation leading to fibrosis and ulceration, pseudoabscess in the bladder
o Granulomas can be seen in the bladder
• Pulmonary involvement common (also has Cor pulmonale)
• Link with bladder carcinoma (squamous cell carcinoma)
Diagnosis • Stool Exam: Kato-Katz, DFS, FECT, MIFC
o For S. japonicum and S. mansoni
• Modified Acid-Fast Staining
o Stain the egg-shell (structure with the acid-fast property)
o Positive: S. japonicum, S. mansoni, and S. intercalatum
o Positive result: red color
o Modified Ziehl-Neelsen
o Negative: S. haematobium
• Detection of eggs in urine for S. haematobium
o Best to collect urine from 12 pm to 3 pm (time when there is highest recovery)
o Concentration techniques: Nucleopore Filtration Technique
o Centrifuge urine, analyze the sediment
• Faust-Meleney’s Egg Hatching Technique
o Determine the viability of the egg
o Positive result: egg hatches, releasing the miracidium
o Use a specialized flask, cover with dark paper, put egg and distilled water in the flask
o Put a flashlight at the opening of the flask, miracidium swims towards the light
• Rectal snips and imprints (most sensitive and most invasive)
• Rectal or Liver Biopsy
• Immunodiagnosis/Serologic tests
o Intradermal tests using adult worm extracts
▪ Fairley’s Test
▪ Positive result: wheal and flare reaction
o Indirect hemagglutination
o ELISA
o Detection of antigens (CCA and CAA, SEA)
▪ CCA: circulating cathodic antigen
▪ CAA: circulating anodic antigen
▪ SEA: soluble egg antigen
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o COPT (Circumoval Precipitin Test)
▪ Confirmatory test/Definitive test in the Philippines
▪ Positive result: bleb or septate precipitates
▪ Sample: serum
▪ Find antibodies against Schistosoma
▪ Reagent: Lyophilized schistosome egg (serves as antigen)
▪ Step 1: Place few amounts of lyophilized egg on glass slide
▪ Step 2: Place serum sample
▪ Step 3: Mix using applicator sick
▪ Step 5: On four corners of the size of a cover slip, place crushed/ground glass
or a drop of nail polish
▪ Step 6: Place the cover slip on top of the glass/nail polish (tuntungan, so the egg
won’t break)
▪ Step 7: Use melted paraffin to seal the edges of the cover slip
▪ Incubate at 37 degrees Celsius for 24-48 hours
▪ Antibody will react with the antigen, form a bleb (structure that grew on the egg)
• Molecular Methods
• Use a rabbit
o Historical notes from Sir Flores! 😊 (They did this daw dati)
o Use infected snails
o Put crushed snails on shaved underbelly of rabbit
o Kill rabbit after a few months, drain all the blood
o Get the lyophilized egg
Epidemiology • 28 endemic provinces in the Philippines
• Covering 190 endemic municipalities
• National Prevalence Rate = 2.5% (DOH, 2008)
• Mindanao: 60%, Visayas: 45%, Luzon: 37.5%
• Region
o CARAGA: highest, 1.63%, Region 8: 1.5%
• Province
o Agusan Del Sur: highest with 3.95%
• Higher among males (occupational hazards)
• Higher among adults
• Occur with other helminthic infections
o STHs (Hookworms and Schistosoma same MOT)
• Reported new foci in Cagayan valley and Negros Occidental (new endemic areas)
• More common during wet months (snails more abundant during this time, people have more
contact with water)
Treatment • DOC: Praziquantel
• For S. haematobium: alternative drug is Metrifonate
Other Blood Flukes Schistosoma intercalatum
• Occurs in Western and Central Africa
• Snail: Bulinus
• Eggs: similar to S. haematobium, there is curvature of the spine, presence of central-bulge
o Hour Glass in Appearance
• Found in the stool
Schistosoma mekongki
• Eggs smaller than S. japonicum
• Milder infection (similar manifestation with S. japonicum)
• Intermediate hosts: Neotricula, Lithoglyphopsis
• Found in Cambodia
• Reservoir host: pigs
Avian Schistosomes – cause swimmer’s itch, lake itch, duck itch, Schistosoma of birds
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LUNG FLUKES
PARAGONIMUS WESTERMANI
• Life cycle almost the same with the general life cycle
• Ingest the crab with the metacercaria
• Metacercaria released in the duodenum (excyst in the duodenum)
• Penetrate intestinal lining (until it reaches the body cavity, peritoneal
wall)
• Worms may not reach the lungs, may go to other parts of the body
(erratic)
• Worm wanders then embeds itself in the abdominal wall
• Then worm returns to coelom and migrates to pleural cavity
o Juvenile diploid worm wanders in the pleural spaces until it
finds one or several diploid worms
o Pair or group then migrates into the lung parenchyma to
develop into adults
o Juvenile triploid worms (Japan, Korea, Taiwan) can establish
themselves in the lung parenchyma without a mate
• Found in sputum or feces
• Embryonate in water
• Miracidium passes through one sporocyst and two redial stages of development
• Cercaria penetrates soft parts of crustacean and encysts as a metacercaria in the gills, body muscles, viscera, or legs
• Immature worm traverses through intestinal wall into peritoneal cavity
• Same banana
Paragonimus westermani
• Common name: Oriental Lung Fluke
• Zoonotic: humans and other animals can serve as definitive and reservoir host
• Habitat: lungs (encysted in lung tissue)
• Paratenic host: boars (harbor larval stage)
• Reservoir hosts: dogs, cats, field rats
• 1st intermediate host: Antemelania asperata (old name Brotia asperata) and Antemelania dactylus (snail)
• 2nd intermediate host: Sundathelphusa philippina, old name: Parathelphusa grapsoides (freshwater/mountain crab)
• IS: metacercaria
• MOT: ingestion of uncooked or undercooked crabs with metacercaria
• P. siamensis: in PH, only been identified in cats
• Cercaria: covered with spines, has an ellipsoidal body, and a small tail
o Stylet present at dorsal side of oral sucker
• Life span of up to 20 years
• Cysteine proteases: play an important role in development of young parasites (involved in metacercarial excystment, tissue
invasion, and immune modulation of the host)
o those with masses 27 and 28 kD: Cleaves IgG therefore creating a zone of immune privilege around the worm
o additional masses of 15, 17, and 53 kD are expressed as juvenile parasite moves towards the lungs
Adult worm • Reddish brown, coffee bean shaped
• Rounded anteriorly and slightly tapered posteriorly
• Found in pairs or threes in fibrotic capsules of the lung
o They undergo reproduction in pairs or in threes
o Capsule: small hole/covering where the egg will be released
• Adult firmly attached to the lung tissue
o Can cause fibrosis (blood in lungs)
o Even if you cough, will not go out
• Presence of spines on tegument (covering)
• Intestinal ceca: wavy
• Testes: lobed, usually 2
o Arranged in opposite (left and right)
o Important characteristic for parasite identification
• Ovary: anterior to testes and posterior to ventral sucker, has six long unbranched lobes
Eggs • Golden brown, operculated, big and large
• Ridge: opercular shoulder
• Presence of abopercular thickening (opposite operculum)
o Characteristic of Paragonimus
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• Immature when released
o Embryonates in water, moist soil, or leached feces
o Takes 2-7 weeks to embryonate
Disease Lung Fluke Disease, Pulmonary Distomiasis, Endemic Hemoptysis, or Parasitic Hemoptysis, Paragonimiasis (signs
and symptoms mistaken for TB)
• Early stages: asymptomatic
• Heavy infections: dry cough/chronic cough, blood stained or rust colored sputum (fishy odor)
• May mimic signs and symptoms of TB, chronic bronchitis, and bronchial asthma
o Living in an endemic area, have to be tested both for TB and Paragonimus
• Erratic Paragonimiasis
o Aberrant or erratic worms
o Can go to heart and brain (brain: meningitis, seizures)
o Localization in the abdominal wall and cavity, mesenteric lymph nodes, pericardium, myocardium,
cerebral involvement may occur
o Cutaneous paragonimiasis: slow-moving, nodular lesion in subcutaneous tissue on abdomen or
chest is seen
o Cerebral paragonimiasis: migrate from pleural cavity to cranial cavity through the jugular vein, can
cause congestion, vasculitis, capillary rupture, infarction, hemorrhage, and necrosis
• Pathology: adult worms provoke a granulomatous reaction (leading to formation of fibrotic encapsulation)
• Chronic stage: liquefaction necrosis and fibrinous gliosis
• Serious sequelae: chronic bronchiectasis and pleural fibrosis (secondary to severe parenchymal and
pleural damage)
• Elevated eosinophils and IgE
Diagnosis • Detection of eggs
o Stool: FECT, Kato-Katz
o Sputum
• X-ray: nonspecific
o Appearance of TB and Paragonimiasis is the same in x-ray
o Focal areas in lung tissue
o Whitish part in the lung tissue
• CT scan, MRI
o Characteristic finding: conglomerated, multiple, ring-enhancing lesions (grape-cluster
appearance)
• Serology: complement fixation, intradermal test, ELISA, immunoblot
o Classic and well-known method: complement fixation
▪ Find the complement-fixing antibodies
▪ High sensitivity
• Microscopy
• Double diffusion in agarose gel
• Immunoelectrophoresis
• Loop-mediated Isothermal Amplification (LAMP) test
o Rapid amplification of deoxyribonucleic acid under isothermal conditions
o Uses DNA polymerase with strand-displacement activity
o Magnesium pyrophosphate: reaction by-product
• Preferred specimen: sputum
• Viscous, thick, and hard to process
• Before examination, add mucolytic agent (3% NaOH)
• Centrifuge after, examine sediment
• Lung biopsy can also be done (but invasive)
• Stool: can be examined if patient swallows sputum
Epidemiology • Global distribution of freshwater crabs
• Endemic areas: Japan, South Korea, Thailand, Taiwan, China, PH
• Endemic Foci in PH: Leyte, Sorsogon, Mindoro, Camarines, Samar, Davao, Cotabato, Basilan,
Zamboanga del Norte, Davao Oriental
• Pulmonary TB must be ruled out (especially in endemic areas)
• PTB may coexist with Paragonimiasis
• Transmission mainly due to food preparation practices
• Dietary habits and presence of snail hosts could be related to endemicity
o Eat raw or undercooked crabs, juice of the crab
• Reservoir hosts: dogs, cats, rodents
• Paratenic hosts: boars and pigs
Treatment • DOC: Praziquantel
• Alternative drug: Bithionol
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INTESTINAL FLUKES
FASCIOLOPSIS BUSKI
• Life cycle starts with eating the water plant with the metacercaria
• Metacercaria goes to duodenum, becomes adults here
• Produce eggs, will be found in the stool
• Eggs are unembryonated
• Take 2 weeks to embryonate in fresh water
• Miracidium penetrate snail
• Same banana
Fasciolopsis buski
•Common name: Giant Intestinal Fluke
•Final host: humans
•Reservoir hosts: pigs, dogs, rabbits
•Habitat: Duodenum and jejunum (small intestine)
•Largest intestinal fluke
•1st intermediate host: Segmentina or Hippeutis
•2nd intermediate host: water plants
o Trapa bicornis (water caltrop)
o Eliocharis tuberosa (water chestnut)
o Ipomea aquatica (water morning glory)
o Ipomea obscura (kangkong)
o Nymphaea lotus (lotus)
• IS: metacercaria
• MOT: ingestion of encysted metacercariae from aquatic plants
• Get infection if water plants are raw/undercooked
Adult Worms • Elongated, oval
• Whitish in fresh specimen
• Ventral sucker: larger than oral sucker
• No cephalic cone (pyramidal structure)
• Intestinal ceca simple and unbranched
o Reach up to posterior end
• Dendritic testes arranged in tandem
• Branched ovary, lies to right of midline
• Fine vitelline follicles situated throughout lateral margin
• Life span: 1 year
Eggs • Large and operculated
• Indistinguishable from Fasciola
• unembryonated
• Hen’s egg appearance
Disease Fasciolopsiasis
• Parasite attached to small intestine
• Pathology due to adult
• Traumatic
o Inflammation and ulceration >> bleeding
o Gland abscesses in mucosa
• Obstructive
o Heavy infections
• Toxic
o Poisons people
o Worm metabolites
o Allergic reactions
o Death
• Marked eosinophilia
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• Malabsorption: of vitamins (B12)
Diagnosis • DFS, FECT, Kato-Katz (eggs indistinguishable from Fasciola)
• Patient history
• Check eating habits
• Clinical impression
Epidemiology • Endemic in: South East Asia, China, Korea, India, Bangladesh
• No local transmission yet
• Reservoir hosts: pigs, dogs, rabbits
Treatment DOC: Praziquantel
ECHINOSTOMA ILOCANUM
Echinostoma ilocanum
•Common name: Garrison’s Fluke
•Final host: humans
•Reservoir hosts: dogs, cats, rats, pigs
•Habitat: small intestine
•1st intermediate host: Gyraulus convexiusculus and Hippeutis umbilicalis
•2nd intermediate host: Pila luzonica (kuhol) and Vivipara angularis (susong pampang)
•MOT: ingestion of metacercariae encysted in snails
•IS: metacercaria
Adult Worms • Reddish gray, tapers at the posterior end (thinner)
• elongated
• Characteristic circumoral disk (with spines surrounding the oral sucker)
o 49-51 collar spines
o Aids in attachment
o Bloody diarrhea attributed to this
• Testes deeply bilobed and in tandem
• Intestinal ceca are simple and unbranched
Eggs • Straw colored or light brown, operculated, ovoid
• unembryonated
• Less prominent operculum, dot like appearance
• size: Fasciolopsis bigger
Disease Echinostomiasis
• Inflammation at site of attachment
• Heavy infections
o Diarrhea (bloody) and abdominal pain
• Intoxication: metabolites (causes poisoning)
Diagnosis Egg (from stool)
• FECT, Kato-Katz (eggs resemble F. buski, smaller compared to F. buski)
Epidemiology • Endemic in: Northern Luzon, Leyte, Samar, provinces in the Philippines, Ilocos
• High incidence in rainy weather
• Eating habits and practices
• 2nd intermediate host abundant in rice paddies during the rainy season
• Important Reservoir hosts: rats
Treatment DOC: Praziquantel
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Artyfechinostomum malayanum
• Similar to E. ilocanum
• Adult worms are bigger
o Possess 43-45 collar spines
o 2 large testes (6-9 lobes in tandem)
• Eggs: larger, golden brown, operculated
• 1st intermediate host: unknown (only known that it’s a snail, species is unknown, but it is similar to
Echinostoma)
• 2nd intermediate host: Lymnaea cumingiana (birabid)
o Ampullarius canaliculatus
HETEROPHYID WORMS
Heterophyid worms
• Final host: man, birds, various fish-eating mammals
• MOT: ingestion of encysted metacercariae in fish
• Habitat: small intestine
• 1st intermediate host: brackish water or marine species snails
o Melania juncea: H. taichui
o Pironella: H. heterophyes
o Semisulcospira: M. yokogawai
o Procerovum calderoni: Thiaria riquetti (from the book)
• 2nd intermediate host: brackish and salt water fish; tilapia
o Mugil cephalus (mullet, for H. heterophyes)
• IS: metacercaria
• Metagonimus yokogawai: Most common intestinal fluke in SEA or Far East
Adult worms • Elongated, oval, or pyriform (pear-shaped)
• Very small
• Tegument: fine scale like spines
• Third sucker (gonotyl) seen in H. heterophyes
• Testes arrangement is varied (depends on species)
• Ovary is globular or lobed
• Short life span (<1 year)
• Metagonimus yokogawai
o Testes: large and oval (2)
o Bit larger than H. heterophyes
Eggs • Light brown color, ovoid, operculated, small
• Embryonated
• No abopercular protruberance
• H. taichui: light striae pattern
Disease • local inflammation at site of attachment
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• colicky pain and mild diarrhea
• peptic ulcer diseases and acid peptide disease
• burrow deep into the intestinal wall (because they are very small)
o eggs can be spilled into the blood stream and carried to different parts (brain, spinal cord,
heart) forming granulomas
o can lead to extra-intestinal infections
• deposition of eggs in vital organs (heart, brain, spinal cord)
• diagnosis most of the time after death
Diagnosis Egg (from stool)
• FECT, Kato-Katz (more efficient)
• Difficult to distinguish
• No abopercular protruberance
Adults
• Autopsy
• Cardiac heterophydiasis: mistaken as Cardiac Beri-beri
Epidemiology • Worldwide distribution
• Egypt, Greece, Israel, Western India, Central and South China, Japan, Korea, Taiwan, Philippines
• Fish-eating countries
• Nonspecific to its hosts
• Reservoir hosts: Dogs, cats, birds
• Locally: low prevalence and spotty
o Compostella valley: 31% prevalence (mostly due to H. taichui)
o Emerging public health problem
o Cases come from provinces in Mindanao
Treatment and • DOC: Praziquantel
Prevention and • Thorough cooking of fish
Control • Proper sanitation
• Health education
Other intestinal Flukes
• Gastrodiscoides hominis
o Final host: man
o Reservoir host: pigs
o Habitat: colon
o 1st intermediate host: Helicorbis coenosus
o 2nd IH: water plants
o Adult worm
▪ Pink/bright pink in appearance
▪ Conical anterior portion
▪ Prominent and notched ventral sucker
o Eggs: operculated, immature, greenish brownish
o Disease: asymptomatic in light infections, mucus diarrhea in heavy infections (abundance of mucus in stool)
o Found in Asia and India
LIVER FLUKES
FASCIOLA SPP.
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Fasciola
• Final host: sheep, cattle, and other herbivores (humans infected occasionally)
• Habitat: biliary passages of liver
• 1st intermediate host: Lymnaea philippinensis and L. auricularia rubiginosa
o From the book:
▪ Europe and North Asia: Lymnaea truncatula
▪ North America: Lymnaea bulmoides
▪ Australia: Lymnaea tomentosa
▪ Indian subcontinent: Lymnaea acuminate
▪ Africa: Lymnaea natalensis
• 2nd intermediate host: Aquatic plants
o Ipomea obscura (kangkong, swamp cabbage)
o Nasturtium officinale (water cress)
• MOT: ingestion of undercooked or raw aquatic plants containing the metacercaria
• Eggs reported as Fasciola egg
• F. hepatica: found in cold and temperate countries, also found in sheep and herbivores
• F. gigantica: more common in tropical countries
• very few among humans
o have economic implications (livestock)
F. hepatica F. gigantica
Common name Sheep Liver Fluke, Temperate Liver Fluke Tropical liver fluke
Adult Worms • Large broad, flat body • Longer but narrower (slender)
• Cephalic cone and prominent shoulders • Shorter cephalic cone and less developed
• Intestinal ceca highly branched shoulders
• Testes (2): highly branched, no longer • Testes: branching
described because highly branched • Ovary: branches longer and more numerous
• Ovary: dendritic
• Uterus: coiled and short
Eggs • Large, ovoidal • Bigger
• Brownish
• Operculated
• Immature when released
• Contains large mass of vitelline cells
Disease Fascioliasis
• Acute stage: larval migration and Worm maturation
o Fever, jaundice, pain, anorexia, hepatomegaly
▪ Jaundice due to obstruction in the bile ducts
• Impedes flow of bilirubin to the intestines, bilirubin accumulates in the liver
• Causes yellowing of skin and sclera of eyes
• Not a disease, only a sign
o Triad of high fever, hepatomegaly and marked eosinophilia
▪ Three important manifestations
o Occurrence of liver rot
▪ Among animals
▪ Tissue destruction of liver
• Chronic stage: persistence of the adults in the biliary ducts
o Obstruction and inflammation leading to fibrosis or cirrhosis
• Other complications: hemobilia, biliary cirrhosis, lithiasis of bile ducts or gall bladder, acute pancreatitis
• During migration from intestine to liver, parasite may wander or be carried by the blood to ectopic sites
(lungs, subcutaneous tissue, brain, etc.)
• Halzoun/Marrara: temporary lodgement of the fluke in the pharynx
o Ingestion of raw liver (flukes still there)
o Mediterranean countries
o Obstruction of pharynx (attached to the pharynx), leads to suffocation
• Pseudofascioliasis
o Spurious infection (not true infections)
o Ingestion of livers (cooked) containing eggs
o False positive infection (the eggs won’t hatch)
o Remedy: liver free diet for 3 days
Diagnosis • Egg (from stool)
o FECT, Kato-Katz
o Difficult to distinguish from F. buski
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o Report as Fasciola egg
• Serology: low specificity
o ELISA, Western Blot
• Molecular methods: RFLPs (Restriction Fragment Length Polymorphism)
o Exploits variations in homologous DNA sequences
• Radiography
o CT scan: multiple confluent, hypodense nodules, and tunnel-like branching hypodense tracts
o Hepatic sonography: small clustered hypoechoic lesions with poorly defined contours and
hypoechoic nodular lesions
▪ Oval-shaped, leaf-like, snail-like echogenic structures with no acoustic shadowing
• Endoscopic retrograde cholangiopancreatography (ERCP)
• Laparotomy
• Diet History
Epidemiology • Worldwide distribution
• Estimated 2.4 million people affected excluding Asia
• 80 million at risk of infection
• F. gigantica dominant species locally
• human infections are sporadic
• Reservoir hosts: hares and rabbits
• no data on disease burden in Asia
• Vietnam: increased number of cases
Treatment • DOC: Triclabendazole
• Bithionol
Clonorchis Opisthorchis
DICROCOELIUM DENDRITICUM
Dicrocoelium dendriticum
•Common name: Lanceolate Fluke or Lancet Fluke
•AKA: Fasciola dendriticum or Fasciola lanceolata
•Final host: herbivores (ruminents), humans may also be infected
o Among cows, sheep, and cattle
o Humans just accidental hosts
• Habitat: Bile Duct, Liver
• 1st intermediate host: Cionella lubrica (snail)
• 2nd intermediate host: Formica fusca (ants)
• MOT: ingestion of ants containing metacercaria
Adults • Blade like, lancet like
• Aspinous (no spines on tegument)
• Testes: located in anterior 1/3
• Ventral sucker bigger than oral sucker
Eggs • Dark brown, thick shelled, large operculum
• Embryonated
Disease • usually asymptomatic
• Symptoms appear if you ingest more ants
• heavy infections: enlargement of bile ducts and hyperplasia of epithelium, may lead to cirrhosis
Diagnosis Stool exam
Treatment DOC: Praziquantel
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PANCREATIC FLUKE
EURYTREMA PANCREATICUM
Eurytrema pancreaticum
•Pancreatic fluke
•Final host: hogs, sheep, goat, cattle
•Accidental host: human
•Habitat: biliary ducts and pancreatic ducts
•1st IH: Macrochlamys indica (snail
•2nd IH: Technomyrmex deterquens (ant), Grasshoppers
•MOT: ingest ant or grasshopper with metacercaria
Adult Worms • Leaf shaped
• Ruffled border or body margin
• 2 notched testis
• 1 notched ovary
Eggs • Similar to lancet fluke
• Operculated
• Embryonated once released
Disease Eurytremiasis
• Destruction of pancreas
o Can cause Type I Diabetes Mellitus
• Chronic granulomatous pancreatitis
• Enlargement of pancreas
Diagnosis Stool exam (egg)
Treatment DOC: Praziquantel
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CESTODES
• Phylum Platyhelminthes, Class Cestoda ▪ For D. latum (has two bothria, hence
• Cestode: comes from Greek word meaning “girdle” or Diphyllobothrium)
“ribbon” ▪ No rostellum, no hooks, no suckers
o Have a tape/ribbon like appearance o Rostellum – protruding structure where hooks are
• Tapeworms attached
• Adult worms appear as flat and ribbon like ▪ Armed – has hooks/hooklets
• All are monoecious (hermaphrodites) ▪ Unarmed – no hooks
• No mouth and gastrointestinal tract ▪ Not all tapeworms have rostellum
o Obtain nutrients via absorption and diffusion through o Neck – region of growth, where proglottids arise
their tegument o Proglottids: tapeworm segments
▪ Immature
• Adult worms inhabit the small intestine
• Eggs are non-operculated, embryonated • Most proximal (closest to neck)
o Except for Diphyllobothrium latum (operculated and • Still developing, no reproductive structures
unembryonated) ▪ Mature
• Fresh specimens appear whitish/creamy • Presence of well-developed reproductive
• Tegument – body covering structures
o Glycocalyx – carbohydrate rich, useful for protection • Uterus, ovaries, testes, vitellaria, vas
o Possess microthrices (microthrix) deferens (which connect to the genital pore)
▪ Similar to microvilli, useful for absorption ▪ Gravid/Ripe
• Most distal from neck
• Filled with eggs
• Testes and ovaries sometimes not seen
o Strobila – chain of proglottids
▪ Strobilization/strobulation (process of formation of
proglottids)
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Vitellaria (for egg shell • Diffused with many follicles Compact and separate
production) • Dispersed, not compact
Gravid Proglottid • All reproductive organs still present • Degenerate reproductive organs
• Rosette uterus • Only uterus and its lateral branches seen
Intermediate Hosts • 1st: Copepods • 1 IH: lower forms of animals, arthropods, man
• 2nd: Freshwater fish • Only 1 (but some do not require intermediate
• More complicated life cycle hosts, like Hymenolepis nana)
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EGGS • Egg becomes coracidium then Procercoid
o Procercoid more elongated
o Cercomer located on distal part of Procercoid
▪ Used for attachment
▪ Has 6 hooklets
• Procercoid becomes Plerocercoid
o Infective stage to humans
o Found in fish
o Causes Sparganosis (called the sparganum) –
larval invasion, multi-organ infection
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Life Cycle
Ingest raw or undercooked fish • Plerocercoid larva released, goes to small intestine
• Plerocercoid larva attaches to lining of small intestine
• Larva becomes adult tapeworm
Adult tapeworm produces eggs • Prolific parasite: produces around a million eggs
• Eggs and proglottids found in the stool (egg is unembryonated)
Egg goes to freshwater • Takes 7-10 or 8-12 days to embryonate
• Egg hatches to release coracidium
Copepods (Cyclops) ingest coracidium • Coracidium becomes procercoid larva inside copepod
• Copepod ingested by another fish
• Becomes plerocercoid larva inside
Plerocercoid larva found in fish • Ingested by humans
Diphyllobothrium latum
• Common name: Broad or Fish Tapeworm (one of largest and longest tapeworms, can become 10-25 meters)
• Final Host: Man
• Reservoir host: Dogs, cats, other fish-eating mammals
• Paratenic Host: Carnivorous Fish (can eat small fish with the larva)
• Habitat: small intestine (ileum)
• 1st Intermediate host: Copepods (Cyclops and Diaptomus)
• 2nd Intermediate host: Freshwater fish (salmon, trout, pike, ruff, perch, etc.)
• Infective stage: Plerocercoid
• Diagnostic Stage: Egg
• MOT: ingest raw, undercooked, or pickled freshwater fish with plerocercoid
Adult Worm • Presence of rosette uterus
• Whitish/milkish
• Scolex: Bothriate (two, located ventrally and dorsally)
• Proglottids disintegrate only when segment has completed its reproductive function
Egg • Operculated and unembryonated
• Opposite operculum: knob-like thickening
• Mistaken for P. westermani
o Both operculated
o Size: Paragonimus bigger than Diphyllobothrium
o Paragonimus: contains abopercular thickening
o Paragonimus is asymmetrical, Diphyllobothrium is symmetrical
o Paragonimus have the opercular shoulder, Diphyllobothrium
does not have
Disease Diphyllobothriasis
• Asymptomatic in most persons
• Obstruction, diarrhea, or anemia have been reported
o Heavy infections: Megaloblastic Anemia due to deficiency of vitamin B12
▪ Hyperchromic, with thrombocytopenia and leukopenia
o Anemia mistaken for Pernicious anemia (B12 deficiency)
o Bothriocephalus anemia vs true pernicious anemia
▪ Both cases: large RBCs on blood smears
▪ Test for Achlorhydria (absence of HCl in gastric secretions), only found in pernicious
▪ Pernicious anemia: autoimmune disease due to problem with parietal cells
• Nonspecific abdominal symptoms
Epidemiology • Occurs in Northern Temperate Areas where raw, pickled, or inadequately cooked fish are eaten
• Found in fish-eating countries (Finland, Japan, Europe, Chile, North America, Norway)
o Especially in Scandinavia
• Finnish people: genetic predisposition to Pernicious anemia
Diagnosis • Demonstration of Eggs (FECT, Kato-Katz DFS)
• Demonstration of Proglottids
• Travel history and diet (may suggest Diphyllobothriasis)
Treatment and • DOC: Praziquantel
Prevention • Niclosamide: may be used, but side effects may be seen
• Cook fish thoroughly
• Store fish properly (Can kill it at a very high and very low temperature, -18C kills plerocercoid larva)
• Environmental sanitation and Health Education
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Sparganosis
• Due to larval forms of D. latum and Spirometra
• Humans are technically intermediate hosts here
o We ingest infective stage inside Cyclops (larval forms, procercoid is accidentally ingested)
o Ingestion of raw infected flesh of amphibians and reptiles
• Procercoid becomes plerocercoid inside the human
• Plerocercoid also known as Sparganum
• Larval stages of parasite are recovered in the different organs
• May cause local inflammation and eosinophilia
• May also get sparganosis if fond of eating raw pork, meat, amphibians, frogs, herbal medicine
• Diagnosis: surgical removal of worms
• Treatment: Surgery, Praziquantel
ORDER CYCLOPHYLLIDEA
TAENIA SPP.
Life Cycle
MOT • Cysticercus goes to small intestine (attaches here)
• T. solium: ingest pig • Proglottids and eggs seen in stool sample
• T. saginata: ingest cattle
Pig and cattle ingest egg • Oncosphere is released
• Oncosphere attaches to the muscle, becomes Cysticercus
Cysticercosis • Humans accidentally ingest egg
• Oncosphere released and deposited to different vital organs (muscle, brain)
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Taenia spp.
• T. solium, T. saginata, T. saginata asiatica (common in Asian countries, PH and Taiwan)
• Final Host: Humans
• Habitat: small intestine
T. solium T. saginata
Common name Pork Tapeworm Beef Tapeworm
Intermediate Host Pigs, Humans (during Cysticercosis) Cattle (humans not an IH, no Cysticercosis)
Infective Stage Cysticercus cellulosae (armed) Cysticercus bovis (unarmed)
• Also known as the Bladder Worm • Also called Bladder Worm
• Egg also infective (during Cysticercosis)
• Cysticercus cellulosae found in pork (pork is
referred to as “Measly Pork”)
• Egg can be found in salad and vegetables
MOT Ingestion of measly pork or egg Ingestion of raw or undercooked beef
Adult Worm • Globular scolex • Whitish opaque
• Armed rostellum (2 rows of hooks, each row • Cuboidal
with 25-30 hooks) • Longer (4-10, up to 25 m)
• Short neck • No rostellum
• Length: 2-3 meters, up to 8 meters
Proglottid Mature Proglottid Mature Proglottid
• Square shaped
• Ovary: Bilobed
• Ovary: Trilobed ovary • Testes more scattered
• Wider than tall (“Squarish”) • Uterus: median, club-shaped
• Smaller number of testes • Vagina has a sphincter (book)
Gravid Proglottid • Genital pore: irregularly alternate
• Only uterus seen Gravid Proglottid
• 5-13 lateral uterine branches (finger-like) • Longer than wide
• 15-20 tree-like uterine branches
HYMENOLEPIS SPP.
Life Cycle
MOT: accidental ingestion of flea or • Larva released, larva attaches to small intestine
beetle o Becomes adult worms here
Stool: eggs and proglottids • Goes to environment
• Ingested by IH
• H. nana: sometimes IH not needed
o Indirect life cycle: requires IH
o Direct life cycle: does not need IH
• H. diminuta: only has indirect life cycle (only IS: cysticercoid larva)
Eggs released inside IH • Becomes Cysticercoid larva
*H. nana autoinfection • Accidentally ingests proglottid containing embryonated eggs
*Direct life cycle: ingestion of egg • Becomes larva then adult in small intestine
H. nana H. diminuta
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Hymenolepis spp.
• H. nana
o Smallest tapeworm of man (25-40 mm)
o Most common cestode infection
o Final Host: Humans
• H. diminuta
o Final Host: Rat
o Accidental host: humans
• Habitat: small intestine (upper portion of ileum)
• MOT: ingestion
H. nana H. diminuta
Common name Dwarf Tapeworm Rat Tapeworm
Intermediate Host Wide variety of insects Variety of arthropods
• Ctenocephalides canis (Dog Flea) • Cockroach
• Pulex irritans (Human Flea) • Rat flea
• Xenopsylla cheopsis (Rat Flea) • Flour moths
• Tenebrio (Rice Beetle) • Flour beetles
• Tribolium (Flour Beetle)
Infective Stage 2 infective stages Cysticercoid larva
• Cysticercoid (indirect)
• Embryonated egg (direct)
Adult Worm • Scolex • Larger than H. nana
o Subglobular or rhomboidal • Scolex
o Armed rostellum (20-30 y shaped o Knob shaped
hooklets in a single row) o Unarmed rostellum
• Neck: long and slender
Proglottid Mature Proglottid Mature Proglottid
• 3 ovoid testes
• 1 ovary
• 60 mm (longer than H. nana)
Gravid Proglottid
• 3 ovoid testes
• 1 ovary (bilobed)
Egg
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Larva Same lang (cysticercoid)
Disease H. nana
• Wala lang
• Usually asymptomatic
• Patients may complain of headache, dizziness, anorexia, pruritus of nose and anus, diarrhea,
abdominal pain
• Infected children: may appear restless, irritable, sleep disturbances
o Infections in children resolve spontaneously in adolescence
• Heavy infections: enteritis due to necrosis, desquamation of intestinal epithelial cells
• Regulatory immunity may limit or eventually clear H. nana population spontaneously
H. diminuta
• Wala lang
• Humans just accidental hosts
• Minimal and non-specific
Epidemiology H. nana
• Children usually infected
• Found in warm countries, poor countries, and where sanitation is poor
• Species in mice and rats: H. nana var. fraterna
H. diminuta
• Worldwide distribution
• More common among children
• Infection usually occurs in poor areas with rats
Diagnosis • Stool exam (look for eggs)
Treatment Praziquantel
DIPYLIDIUM CANINUM
Life Cycle
Dog accidentally • Larva released, attach to small
ingest flea with intestine
cysticercoid larva • Larva becomes adult worms
Stool: proglottids • Eggs enclosed in egg packets
with eggs
Flea ingests egg • Eggs becomes cysticercoid larva
Dipylidium caninum
• Double Pored Tapeworm, Dog Tapeworm, Flea Tapeworm, Cucumber Tapeworm
o Proglottid looks like cucumber
• Final Host: dogs, cats
• Accidental host: man
• Habitat: small intestine
• Intermediate hosts
o Ctenocephalides canis
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o Ctenocephalides felis
o Book: also includes Pulex irritans (human flea) and Trichodectes canis (dog louse)
• Infective stage: cysticercoid larva
• MOT: ingestion
Adult Worm • Scolex
o Conical
o 4 suckers
o Retractable armed rostellum (with several rows of hooks)
▪ 1-7 rows of rose thorn-shaped hooklets
Proglottid
• Two genital pores on each side (left and right)
• Has 2 sets of reproductive organs (2 of each)
• Gravid proglottid: looks like a cucumber (size and shape of pumpkin seed)
• Also barrel-shaped
RAILLIETINA GARRISONI
Raillietina garrisoni
• Can be spelled either Raillientina or Raillietina
• Aka Raillietina madagascariensis
• Madagascar Worm
• Common tapeworm of rats
• Belongs to family Davaineidae
• Final host: rats
• Accidental host: man
• Habitat: small intestine
• Intermediate host: Tribolium confusum (beetle)
• Infective stage: Cysticercoid larva
• MOT: ingestion
• Life cycle similar to Hymenolepis
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Adult Worm • Scolex Proglottid • Gravid proglottid: rice grain
appearance
o Subglobular
o Armed rostellum
o 2 alternating rows of hammer shaped
hooklets
o Several rows of spines also surround
rostellum
ECHINOCOCCUS SPP.
Life Cycle
Dog: ingest • Hydatid cyst goes to vital organs
hydatid cyst • Protoscolex inside will attach to
small intestine and become adult
worm
Stool of dog: • Eggs ingested by IH
proglottids and • Eggs hatch and release
eggs oncosphere
• Oncosphere becomes hydatid cyst
Hydatid cyst goes • Human: dead-end host
to vital organs
(liver and lungs)
Echinococcus granulosus
• Other species: E. multilocularis, E. vogeli
• Hydatid Worm
• Smallest tapeworm of dogs (3-6 mm)
• Belong to family Taeniidae
• Final host: Canines (Dogs)
• Habitat: small intestine
• Intermediate host: sheep, goat, swine, cattle, horses, camel, humans (accidental)
• Infective stage: hydatid cyst
• MOT: ingestion
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Adult Worm • Scolex Egg • Similar to Taenia
o Pyriform
o Armed rostellum
o 4 suckers
• Proglottid: composed of
3 proglottids only (immature,
mature, and gravid)
Hydatid cyst
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• E. vogeli: Central and South America
Diagnosis • Stool exam not performed (because it is found in the vital organs)
• X-ray
• Ultrasound, CT Scan
• Surgery
• Serology
o Bentonite Flocculation Test
o Casoni Intradermal Test (skin test)
▪ Positive: wheal and flare reaction
▪ Antigen from hydatid fluid injected to skin
▪ Then you develop an immune reaction
▪ Detects previous exposure to parasite
o ELISA
Treatment • Surgical resection (be careful kasi when it ruptures, can disseminate to other organs)
• Albendazole
• Praziquantel
• PAIR (Puncture, Aspirate, Inject, Reaspirate)
o Inject scolicidal agent (95% Ethanol, hypertonic solutions, Hibitane)
Other Echinococcus species (uncommon because of sylvatic life cycle)
• E. multilocularis
o FH: foxes
o IH: rodents (voles, lemmings, shrews, mice)
o Causes Alveolar Echinococcosis
▪ Multilocular hydatid cyst produced (has many compartments)
• Has no protoscolex inside
• Produce gelly-like substance inside
• Mistaken as carcinomas (cancer)
• E. vogeli
o FH: bush dogs and dogs
o IH: rodents
o Multilocular hydatid cyst
o Causes polycystic echinococcosis (w/ E. oligarthrus)
MULTICEPS MULTICEPS
Life Cycle
Dog ingest • Coenurus develops to adult worm in
herbivores with small intestine
Coenurus • Proglottids and eggs released
Humans/IH • Larva develops
ingest egg • Larva can disseminate to different
vital organs
o Can go to brain
o Can go to eyes (can cause
blindness and ocular
infections)
• Human releases Coenurus
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Multiceps multiceps
• Formerly known as Taenia multiceps
• Gid Worm, Dog Sheep Tapeworm
• FH: dogs and other canines (foxes, wolves)
• Habitat: small intestine
• Intermediate host: herbivores (sheep), man (accidental)
• Infective stage: Coenurus
• MOT: ingestion
Adult Worm • Scolex Egg • Similar to Taenia (take note of
o Pear shaped patient history before reporting)
o 2 rows of 22-30 hooks
• Proglottids
o 1 lateral genital pore
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LABORATORY DIAGNOSIS
Common specimens used to detect parasites include: o Fixation time: minimum of 30 minutes
o If immunoassay for E. histolytica/dispar is requested,
• Stool fresh or frozen stools are required
• Blood
• Duodenal material Fixatives
• Sigmoidoscopy material (colon) Formalin • All purpose fixative for helminthes and
• Perianal swab (cellulose acetate) preparation protozoans
• CSF and other sterile fluids like peritoneal, pleural fluid, • 5%: protozoans
• 10%: helminthes
and bronchial washings
• can be used for direct examination (wet
• Tissue and biopsy specimens
mounts), concentration methods (FECT),
• Sputum aqueous formalin for Giardia and
• Urine and genital specimens Cryptosporidium immunoassays,
• Eye specimens sediments of stool fixed in formalin for
• Mouth scrapings and nasal discharges staining of intestinal coccidians (modified
• Skin snips acid fast) and microsporidia (modified
trichrome), and hot formalin (useful for
STOOL preserving helminth eggs)
• disadvantage: not used for making
• Most common specimen submitted permanent stained smears
• Different parasite stages can be detected Merthiolate • composed of merthiolate (thimerosal),
• Container used: wide mouth, water tight, plastic container Iodine iodine, and formalin
with tight fitting lid Formalin • used for most parasite stages for field
• Other containers: Wax-lined carboard (0.24 L) (MIF) studies
• Stool containers should be placed in a plastic bag during • usually used as a wet preparation
transport Polyvinyl • usually combined with Schaudinn’s
Alcohol Solution (which contains Mercuric
• Usually collect 3 specimens in a span of 10 days
(PVA) chloride)
o Done since parasite forms/stages are shed
• plastic powder which acts as an adhesive
intermittently and multiple collection increases
• recommended for permanent stained
sensitivity of detection
smears
o Up to 6 specimens collected in 14 days for detection • can also be used for concentration
of amebiasis methods
o 1st 2 specimens: collected normally • usually incorporated in a two-vial system
o 3rd specimen: collected with the use of cathartic • disadvantage: toxic
• Specimen should be properly labeled Schaudinn’s • for permanent stained smears from fresh
• Medications such as barium, bismuth, laxatives, and fecal specimens
mineral oil can interfere with parasite detection • Gold standard
o Patients taking these substances should defer stool • Excellent for protozoan trophozoites and
collection for a week after the last intake cysts
o These substances can leave crystalline residues Sodium • Softer fixative than mercuric chloride
o Antibiotics or antimalarials: delayed for 2 weeks Acetate • Alternative to PVA and Schaudinn
following therapy Formalin • Used for concentration techniques,
• Amount: 2-5 grams (walnut or thumb sized) of formed modified acid fast techniques, and
stool permanent stained smears
o Diarrheic stool: 5-6 tablespoons • Only requires a single vial
• As much as possible, specimen should be received and • Long shelf life
examined by laboratory as soon as possible • Disadvantage: adhesive property is not
o Liquid or diarrheic stools: within 30 minutes good, use of mayer’s albumin
o Semiformed stools: within 1 hour Modified • Alternative, safer than PVA
o Formed stool: held for 1 day
Polyvinyl • Contains Copper sulfate or Zinc sulfate
o If immediate examination cannot be done, specimen
Alcohol • Used for concentration techniques and
permanent stained smears
may be refrigerated or added with preservatives
• Morphology is not as good as PVA
▪ Refrigeration is only temporary
• Zinc sulfate: provides better results
▪ Never keep stool samples in freezers or
Alternative • Non-toxic fixatives
incubators Single Vial • Free of formalin and mercury
• Preservatives Systems • For concentration techniques and
o Fixatives: substances that preserve the morphology permanent stained smears
and structure of parasite stages (also used to prevent • For use of fecal immunoassays
further development of parasites)
o Recommended fixative to stool ratio: 3:1
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• Ex: ECOFIX, TOTAL-FIX (Universal • Ex: Formalin Ether Concentration
Fixative) Technique (FECT), AECT, Formalin Ethyl
Acetate Concentration Technique
MICROSCOPY: OVA AND PARASITE EXAMINATION (FEACT), MICT
▪ Flotation: allows parasites to float
• Standard procedure performed in stool • Ex: Zinc Sulfate Flotation (uses 33%
• Consists of a macroscopic and microscopic examination ZnSO4, specific gravity is 1.18 to 1.20),
Brine Flotation (supersaturated solution of
MACROSCOPIC EXAMINATION NaCl), Sheather’s Sugar Flotation
• non-recovery of dense or heavy eggs
• Gross examination of stool specimens • Permanent Stains
• Performed on unfixed or fresh stool specimens o the final procedure
• Consistency or Form of Stool o used for confirmation of the presence of a protozoan
o Can determine the potential parasite form (if cyst or cyst or trophozoites
trophozoite) o sample of choice: PVC fixed stool (with Schaudinn’s)
o Can be hard, soft, mushy, loose, diarrheic, watery, o Trichrome (Wheatley Modification)
formed, or semi-formed ▪ Widely used, easy to prepare, has long shelf life
▪ Usually with PVA
• Color
▪ nuclear chromatin (peripheral and karyosome):
o Brown: normal color, pigment responsible for brown
red-purple
color is stercobilin/urobilin
▪ background: blue-green
o Purple, Red, Blue: due to medication
▪ yeast: bright blue green or reddish
o Gray: may indicate bile duct obstruction
▪ cytoplasm: blue green, blue, blue-gray
• Gross Abnormalities
▪ chromatoidal bar: bright to dark red
o May find adult worms, proglottids, pus, mucus, dark
▪ charcot leyden crystals: bright red
colored blood, bright red blood
▪ glycogen vacuole: colorless
o Ryan’s Trichome Stain: used for microsporidia
MICROSCOPIC EXAMINATION
o Periodic Acid Schiff
o Chlorazol Black E
• Visualization of parasite stages (also RBCs, WBCs, o Iron Hematoxylin
macrophages, charcot-leyden crystals, fungi, plant cells, ▪ Time consuming
pollen grains, plant fibers) ▪ Has excellent morphology of intestinal
• Direct Fecal Smear (Direct Wet Preparation) protozoans
o can use a Saline (NSS) or Iodine Mount ▪ Classical method
o Saline (NSS) Mount: can observe trophozoite ▪ Stain should be fresh
motility ▪ Gives the best nuclear detail
▪ Nair’s and Quensel’s Methylene Blue used to ▪ Cytoplasm: blue-gray
observe nuclear details of trophozoite ▪ Nuclei and chromatoidal bar: dark blue or black
o Iodine Mount: NSS + Lugol’s Iodine ▪ RBC: black
▪ Alternative to lugol’s: D’Antoni’s ▪ Charcot Leyden crystals: blue back
▪ Staining of cysts ▪ Glycogen: colorless
▪ Used for cysts only because iodine is toxic to o Specialized Stains
trophozoites ▪ Modified acid fast stain
o Smears should be made thinly ▪ Modified iron hematoxylin (with carbolfuchsin)
o Standard slide: 1x3 inches
o Coverslip: 22x22 mm OTHER TESTS FOR STOOL
o Direct preparation for NSS and Iodine mount is
prepared side by side on the slide
• Kato Thick: for qualitative examination of helminth eggs,
o Sealing can be done using paraffin and petroleum
does not use a calibrated template
jelly or nail polish
• Kato-Katz: for
o Disadvantage: less sensitive, low diagnostic yield
quantitative
• Concentration Methods
examination of
o Parasites are aggregated into a small volume
helminth eggs,
o Removes fecal debris
amount of stool
o Detection of protozoan cysts, oocysts, helminth eggs,
is
and larva
standardized
o Trophozoites are not detected
with a template
o Principles
o Wire mesh
▪ Sedimentation: recommended (has better
is used
recovery)
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o Green cellophane with glycerol is placed on top of o examined with the OIO
the slide o stained using Giemsa’s or Wright’s
▪ Glycerol: clearing agent, removes interfering o other stains: Delafield Hematoxylin (for
substances demonstration of detailed structures of microfilaria)
▪ Cellophane: to reduce eye strain o Thick is for quantification
o Used to classify intensity or severity of disease o Thin is for species identification
• Stoll Egg Count: most widely used dilution egg counting o Examine under LPO to screen for presence of
procedure microfilaria
o 0.1 N NaOH and a stool displacement flask used • Membrane Filtration
o Factor multiplied to 100 to determine egg per gram o Using a millipore filter (pore sizes depends on the
• Baermann Technique: for recovery of Strongyloides species to be detected)
stercoralis o For microfilaria
o Funnel + gauze is used o Uses EDTA or citrated blood diluted with Teepol-
• Coproculture: uses a petri dish Saline Solution (lysing solution)
• Schistosomal Egg Hatching o Stained with Giemsa
• Agar plate method o Membrane filter has a 3-5 um diameter
• Harada-Mori Technique ▪ 5 um for Loa loa
o Uses a conical tube with water ▪ 4 um for W. bancrofti and other small species
o Filter paper with the stool is o Examined initially under 10x
placed inside • Knott Concentration Technique
o Left standing for 7-10 days o For concentrating microfilaria
o Used to identify rhabditiform or o 1 ml of blood (EDTA) + 10 ml 2% formalin
filariform larvae o Centrifuge, then examine the sediment
o Used for Hookworms and o Stained with Giemsa (standard stain for blood
Strongyloides parasites)
• Culture Media for protozoans include • Buffy coat slides
Diamond’s medium, Boeck and • Cultures include NNN (Novy McNeal Nicolle Medium)
Drbohlav’s Locke Egg Serum, and and Chang’s
Modified Thioglycolate medium
• Stool screening done through rapid OTHER SPECIMENS
methods
o Uses kits • CSF: for hemoflagellates, free-living ameba,
o Ex: EIA, DFA Parastrongylus, and Toxoplasma
• Tissue specimens for Trichinella
OTHER INTESTINAL SPECIMENS • Sputum for Paragonimus ova, migrating larva of Ascaris,
E. granulosus hooklets
• Duodenal Material o also used for Protozoa such as E. histolytica, C.
o Used to detect parasites inhabiting the small intestine parvum, E. gingivalis, T. tenax
o Collected by nasogastric intubation or the Enterotest o first morning specimen is used (induction can be
o Parasites observed includes Giardia, done by using 10% sodium chloride or hydrogen
Cryptosporidium, Isospora, Strongyloides, Fasciola, peroxide)
Clonorchis • Urine: for Trichomonas and Schistosoma haematobium
• Sigmoidoscopy • Rectal biopsy for S. japonicum
o Detection of E. histolytica • Eye specimens for Acanthamoeba
o Done using biopsy specimens of the colon • Mouth scrapings for E. gingivalis and T. tenax
• Cellophane Tape Preparation • Nasal discharge for Naegleria
o Specimen of choice for Enterobius vermicularis • Skin snips for Onchocerca volvulus
▪ If stool is used to detect this, only a 5% chance
of recovery RECENT ADVANCES IN DIAGNOSTIC PARASITOLOGY
o Can also be used for Taenia eggs
• Microscopy: parasite concentration technique (like
BLOOD FLOTAC) can be performed prior to microscopy
o Use of UV fluorescent microscope also a
• For systemic or blood borne parasites recent advance
• Wet Preparation: used to detect motility of filaria • Immunodiagnosis: immunofluorescent assays, ELISA,
o used to prepare a stained smear HA, immunoblotting
o collect capillary blood (fingertip or earlobe) • Molecular diagnosis
o no anticoagulant is preferred • Rapid diagnostic tests
• Preparation of Smears (Thin and Thick Smear)
o used to detect malaria
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