I.

Introduction

Like any other multicellular living thing,

leaf structure is made up of layers of
cells. Viewing the leaf under the microscope
shows different types of cells that serve various
functions. Using a microscope, it's possible to
view and identify these cells and how they are
arranged (epidermal cells, spongy cells etc). And
the experiment that was assigned to us was
Rhoeo Discolor.

Rhoeo Discolor (Common Name – Moses-In-The-Cradle) belongs to the

family Commelinaceae. It is a fleshy rhizomes and rosettes of waxy lance-
shaped leaves. Leaves are dark to metallic green above, with glossy purple
underneath.

II. Methodology

A. Setting up the slide

 Gathering of materials.
 Preparation of specimen (Rhoeo Discolor).
 Rotate the revolving nosepiece to the lowest power objective lens.
 Place the Rhoeo Discolor in a slide glass.
 Add a small amount of water.
 Place a glass cover or coverslip over your specimen.
 Mount your specimen onto the stage using its metal clips.
  Rotate the focus knob/coarse adjustment knob until the objective
lens hovers over the slide.

B. Adjusting Light and Focus

 Move the slide until it's focused in the center.
 Adjust the mirror, illuminator, and/or diaphragm for maximum light
exposure.
 Adjust the coarse and fine adjustment knobs until the image is
focused.
 Switch to the next powerful objective lens and make final focus
adjustments.
 After examining, turn the nosepiece back to the lowest power lens,
carefully remove the slide, and place a cover on your microscope.

III. Observation

In the experiment, we can clearly see the epidermis of the

Rhoeo Discolor which covers the outer surface and thus cover all the
plant tissues using only the low-power objective lens (LPO). The
epidermal cells in the epidermis serve as a protective layer not only to
prevents various microorganisms from gaining entrance into the
underlying tissue of leaves and stems, but also prevents excess water
loss.

In using the high-power objective lens (HPO) we can clearly

notice the rhombus-shaped object (violet color) within the cell that is
made up of multiple stomata (green-colored elliptical spot) and guard
cells that are surrounding the stomata which regulate the rate of
transpiration in opening and closing the stomata. In addition, the
different parts of the plant’s cell are visibly seen such as the cell wall
and the cell membrane of the specimen. Its rough texture is
distinguishable as well.

Rhombus shaped

Object (Pink)

Guard

Cell

Stoma

(Green-Colored

Elliptical Spot)

Actual photo (LPO)

BIOLOGY
I
Escoto, Jhageson Lance C.

Amada, Leynon Mark M.

XI - Pegasus
 Documentation

Violet Side LPO (Photo Green Side LPO (Photo taken by

taken by Jhageson) Leynon)

Violet Side HPO (Photo Green Side HPO (Photo taken

taken by Jhageson) by Leynon)