Manual Tecnico 290 PDF

™ ™
3M Attest Auto-reader
Service Manual
Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including
photocopying, microfilming, recording or by any information storage and retrieval system, without permission from 3M Health Care.
Health Care
Attest Auto-reader Service Manual
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Page 2 of 38 3M©2009 78-8078-9168-0
Revision History
Revision Date Page(s) Changed
A 10/16/2001 All
Change: Birth Notice, Manual Released
B 7/16/2003 Deleted Pages 5-13

Updated New Pages 5-14
Updated New Pages 16-21
Change: Eliminated Operator’s Manual Section

Change: Updated Acceptance Test Procedure to match C-05-005002 (MCU-0002) Version 7
Change: Updated BOMs to designate spare parts availability
C 7/18/2005 All
Change: Added drawings and parts list for new 290 case design
Change: Added drawings and parts list for 293/293G Auto-reader
Change: Updated Acceptance Test Procedure to include 293/293G Auto-reader
D 4/21/2009 All
Change: Performed an 11-digit stock number review, update some per ECOs.
Change: Added new “round” cord clip for power supply, 26-1017-3696-0 and deleted the flat
power supply cord clip and screw, 26-1017-2681-3, 26-1014-7689-8, Reference, ECO-05-003550.
Change: Changed the 10 inch ribbon cable (connects the NMOM and display PCB) from 26-1014-
7284-8 to the 12 inch ribbon cable 26-1017-6304-8 for the 293/293G, reference ECO-05-003152.
Change: Added Connectivity Kit stock numbers to the 290 and 290G per ECO-05-003862.
Change: Added stand-offs, Dual Lock, etc. to Service Manual (for shipping purposes for the
293/293G), reference ECO-05-003345.
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3M©2009 78-8078-9168-0 Page 3 of 38
TABLE OF CONTENTS
TROUBLESHOOTING GUIDE
Troubleshooting Guide ……………………………………. 5
ACCEPTANCE TEST PROCEDURE

Special Instructions …………………………………… 7
Assembly/Test Procedure …………………………………… 7
Acceptance Test Procedure …………………………………… 8
Calibration Procedure …………………………………… 10
Preshipment checks/Packout Procedure …………………………………… 11
290/290G Acceptance Test Sheet …………………………………… 13
293/293G Acceptance Test Sheet …………………………………… 15
ILLUSTRATED PARTS LIST

290/290G Assembly …………………………………… 18
293/293G Assembly …………………………………… 22
Frame Assembly …………………………………… 24
Sled Assembly …………………………………… 26
Sled Holder …………………………………… 27
THEORY OF OPERATION
Detailed Discussion …………………………………… 28
SCHEMATIC DIAGRAM
Wiring Diagram …………………………………… 30
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Page 4 of 38 3M©2009 78-8078-9168-0
Please refer to Operator’s Manuals for Normal Operation:

290 Operator’s Manual 78-8078-8977-5 Literature Code 70-2009-4089-1
290G Operator’s Manual 78-8083-0136-6 Literature Code 70-2009-4687-2
In the event of an error code, unplug the Attest 290/290G Auto-reader and plug it back in to initiate the
self-diagnostic test. If error code re-occurs, customer should call for repair or replacement.
The display panel will indicate malfunctions detected by a specific error code. Refer to the following:
Code Problem Solution
C1 Unit Warm-up Caution Allow a 30 minute warm-up after

Incubation block not at temperature Attest 290/290G Auto-reader is plugged in.
RRBI readings are disabled until
The incubator block reaches:
290 60ºC +/-2ºC
290G 37ºC +/-2ºC
C2 RRBI has been removed before incubation Replace RRBI in proper well within 10 seconds.
completed. All three lights for affected RRBI
will light up and alarm will sound if active.
E1 Temperature Control Error Check heater blanket connection

This error will occur if system is no
longer able to control the temperature of
the incubator block.
290 60ºC +/-2ºC
290G 37ºC +/-2ºC
E3 Memory Error. Replace NMOM PCB
E4 Analog-Digital Conversion Error. Replace Sled PCB
E7 Communications Error Check ribbon cable connection between

This error will occur if the internal NMOM and Sled PCB. In addition, check for
communications have failed. power interruptions’ which could cause
communications error, E7
E8 Bulb Error Check for NMOM Software V1.2

This error occurs if the system detects Replace Sled PCB / old bulb
that the bulb is too dim to properly read
RRBI results.
E9 Transport Error Check for loose set screw on sled

This error will occur if the system detects Check for loose set screw on motor drive pulley
a motor failure or positioning problem. Check for loose drive belt
Check bearings for smooth operation
Check for power interruptions’ which could
cause communications error which could
cause E9
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3M©2009 78-8078-9168-0 Page 5 of 38
Trouble Shooting Guide (Continued)
Other Problem Solution
Red, yellow, or green lights do not Specific light burned out – replace Display PCB
illuminate when expected such as Check ribbon cable connection between
following insertion of a RRBI or when NMOM and Display PCB
time remaining button is pressed.
LED flash test does not function Check alignment and connection of
lid switch
Unwanted alarm sounds when Turn alarm off, refer to features

positive detected. Section.
All three lights blink and buzzer sounds. Replace RRBI in incubation/reader
RRBI has been removed from well within 10 seconds to prevent
incubation/reader well prematurely. loss of data collected.
Negative Control Vial
Vial not crushed. Crush vial before inserting into well.
Media did not wet the spore strip. Tap vial on table until media wets spore strip.
Control vial was sterilized. Use a non-sterilized vial as a control.

Check chemical indicator on label.
Unexplained positive
Adhesive residue on vial. Do not place tape or labels on vial.
Test vial is absorbing fluorescent Place the vial so it does not come in
residue from a chemical indicator or direct contact with chemical
tape. indicators or tape.
RRBI placed into a well immediately Allow time for previous BI result to clear.
following removal of previous BI.
Inconsistent Read Time
RRBI not inserted all the way. Ensure RRBI is inserted all the way into the
reader well.
RRBI placed into a well immediately Allow time for previous BI result to clear.
following the removal of previous BI.
Intermittent Power Connection Spread split pin on NMOM power jack to

ensure snug fit with plug.
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Page 6 of 38 3M©2009 78-8078-9168-0
ACCEPTANCE TEST PROCEDURE
1.0 DESCRIPTION: Attest Auto-reader Final Acceptance Specification and Packout
1.1 This procedure covers the following Auto-readers:
Finished Goods # Description
1.1.1 70-2007-2980-7 290 Attest Auto-reader 100VAC

1.1.5 70-2007-3170-4 290G Attest Auto-reader 100VAC
2.0 SPECIAL INSTRUCTIONS:
2.1 SAFETY PRECAUTIONS: Follow facility general safety guidelines for electronic
assembly and testing.
2.2 Standards and specifications given on assembly and component

drawings must be met.
2.3 Hardware fasteners must be fully seated and tight.
2.4 Exterior surfaces must be free from scratches, sharp edges and burrs. Any paint touch-ups
must be neat and blend well with surrounding surface.
2.5 Wiring should be held neatly in place. Wiring should not rub against sharp edges such as
sheet metal ends or screw threads.
2.6 Switches must operate without binding.
2.7 The person performing assembly operations is responsible for meeting the print
requirements. The checksheet is to be filled out by the assembler for each machine.
2.8 The location of the labels is defined on the prints. The labels are to be mounted straight,
without bubbles or lifted edges.
2.9 If a machine fails a test, indicate on the checklist the failure and repair. The unit must be
tested again after the repair for that function. The results of the test after the repair must
be recorded.
2.10 Any/all unexpected error codes displayed during testing must be resolved before
continuing.
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3.0 ASSEMBLY/TEST PROCEDURE:
3.1 In-Process tests
3.1.1 General Workmanship
3.1.1.1 Screw tightness, solder joint quality, wire connections etc
3.1.2 Ribbon Cable Assembly
3.1.2.1 Ensure ribbon cables are inserted fully and routed and secured properly.
3.1.3 Heater Blanket Application
3.1.3.1 Clean outside surface of the incubator block with isopropyl alcohol
prior to attaching the blanket.
3.1.3.2 The blanket must be laid flat with no lifting of the blanket away from
the incubator block. (Inspection to be done prior to placing insulation
on unit.)
3.1.4 Home Sensor Adjustment
3.1.4.1 Remove outer case.

3.1.4.2 Measure and mark the center of the aluminum optics housing with a
pencil or felt-tip pen.
3.1.4.3 Apply power to unit and allow sled to move to home position.
3.1.4.4 View from top of incubator block and ensure that the center of the
optical housing is directly in line with the center of Well #12.
3.1.4.5 If optics is not centered, adjust position of home sensor vane as
necessary and cycle power to re-home sled position.
3.1.5 Sled Spacing Across Incubator Block
3.1.5.1 Unplug motor from NMOM board and slide sled to home position.
3.1.5.2 Adjust spacing between sled optics and edge of incubator block to be
0.040” (+ 0.00”, - 0.025”). Tighten screw to secure incubator block to
frame.
3.1.5.3 Slide sled in front of well #1. Adjust spacing between sled optics and
edge of incubator block to be 0.040” (+ 0.00”, - 0.025”). Tighten screw
to secure incubator block to frame.
3.1.5.4 Slide sled slowly back to home position, ensuring that optics do not
touch incubator block at any point along the full range of travel. At
home position, verify spacing between sled optics and edge of
incubator block to be 0.040” (+ 0.00”, - 0.025”). If not within
specification, go to 7.2.4.2 and repeat process.
3.1.5.5 Plug motor cable back in to connector.
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Page 8 of 38 3M©2009 78-8078-9168-0
STEPS TO REMOVE COVER OF 293/293G AUTO-READER:
1. Remove 4 screws from back cover
2. Remove 8 screws used to mount the top cover
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3. Lift RIGHT side of top cover. Display Ribbon cables are connected on LEFT side of top cover.
4. Un-plug or Re-plug Display Ribbon cables as needed.
LIFT COVER
RIBBON CABLES
ROTARY SWITCHES
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Page 10 of 38 3M©2009 78-8078-9168-0
5. Set rotary switches for all 3 tiers to setting 0 for functional testing.
6. Unplug USB-to-RS232 adapter at the RS232 end and connect computer serial cable for functional
testing of each tier (top, center, bottom).
USB to RS232 ADAPTER
7. After testing:
- Re-plug USB-toRS232 adapters for each tier
- Re-set all three rotary switches based on labeling (1292 or 1294)
- Ensure all Display Ribbon Cables are securely connected
Proper Repairs Prior to Shipping the 293/293G
293/293G Frame Top View

Drill holes on ends as shown for mounting stand-offs.
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3M©2009 78-8078-9168-0 Page 11 of 38
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Proper Repairs Prior to Shipping the 293/293G Continued:
Auto-reader Display Ribbon Cable Disconnection Resolution.

Attach from ribbon cable to ribbon cable connector.
- Proper Repairs Prior to Shipping the 293/293G includes:
Add Dual Locks, Stand-offs, lock nuts and screws as shown below. Reference 293/293G
assembly drawing for full blow-up of stand-offs, etc.
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Page 12 of 38 3M©2009 78-8078-9168-0
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- 293/293G ASSEMBLY
ITEM PART DESCRIPTION QUANTITY
NUM. NUMBER REQUIRED
1 78-8083-1005-2 3M 8422 Polyester Ribbon Connector Tape 3

2 78-8083-1004-5 Alignment pins, for shipping 4
9 26-1017-1620-2 Screw, 8-32 x 0.5”, Pan, Phil, Blk Zn 6
39 70-0705-3010-3 Dual Lock SJ3263 – Round 13/16” 6
40 70-0707-4015-7 Dual Lock SJ3550 – Square 1” by 1” 6
41 26-1017-6006-9 Standoff, 8-32, M/F Hex, 1.25” 6
42 26-1017-3845-5 8-32 KEPS Lock-nuts, Zinc, Hex 6
1
- For Shipping 293/293G:

- Note: Alignment Pins to be placed in well #1 and #12 for each heater block.
-
-
- 4.0 ACCEPTANCE TEST PROCEDURE:
Note: This procedure to be done for 100% of units. For 293/293G Auto-readers, perform each test for each
of the three tiers of incubators (top, center, bottom).
4.1 Error Code Test:
4.1.1 Disconnect power and remove cover.

4.1.2 Set rotary switch to position 1.
4.1.3 Generate a failure condition by one of the following 2 methods:
4.1.3.1. Disconnect heater blanket.
4.1.3.2. Disconnect motor wire connector and move sled off home position
toward Well #1.
4.1.4 Apply power to the unit and verify the error code:
4.1.4.1. "E1" for temperature failure.
4.1.4.2. "E9" for motor movement failure.
4.1.5 Disconnect power, repair failure, apply power, and verify no error code is
displayed.
4.2 Display Verification:

4.2.2 Apply power to the unit.
4.2.3 Verify 7-segment display shows "8.8." on initial power-up as a LED check.
4.2.4 Verify 7-segment display shows "F1" (1291 BIs) as a set-up mode indication.
4.2.5 7-segment display will show "C1", the warm-up caution code, if not up to
temperature.
4.2.6 Open cover on unit and ensure all red, yellow, and green LEDs light.
4.2.7 Insert a BI into any well of the test unit.
4.2.8 Press "time remaining" button and verify that "1H" is shown for the BI.
4.2.9 Wait for the unit to heat up and the first reading to be taken.
4.2.10 Remove the BI and verify that "C2" is displayed and the audible signal sounds.
4.2.11 Re-insert BI within 10 seconds and verify that caution code clears and audible
signal ends.
4.2.12 Press both buttons and verify that period is displayed on the 7-segment display
to signify the audible signal is disabled.
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4.2.13 Remove the BI and verify that "C2" is displayed and the audible signal does not
sound.
4.2.14 Re-insert BI within 10 seconds and verify that caution code clears.
4.2.15 For 293/293G Auto-reader, press both buttons for EACH tier and verify that
period is toggled on/off on the 7-segment display of ALL THREE TIERS
SIMULTANEOUSLY.
4.2.16 Remove BIs.
4.2.17 Remove Power.
4.3 Automated Test System Setup, Manufacturing Test System (MTS)

4.3.2 Plug serial cable into connector on unit.
4.3.3 Cycle power to unit and start Automated Test System Software or select “File,
New” if test system is already running.
4.3.4 Enter serial number of unit being tested.
4.3.5 Perform the following tests:
Note: If any tests fail, re-test once before re-working unit.
4.4 Serial Communications
4.4.1 Select "Serial Communication" test and press "Test"

4.4.2 Verify Successful Result in status display box.
4.5 Lid Test (290/290G Only)
4.5.1 Select "Lid Test" test and press "Test" or press "Next Test".
4.5.2 Open lid and leave open when prompted and press "OK".
4.5.3 Close lid when prompted and press "OK".
4.6 BI Hole Test
4.6.1 Select "BI Hole Test" test and press "Test" or press "Next Test".
4.6.2 Make sure all wells are empty when prompted, close lid, and press "OK".
4.6.3 Insert BIs into all wells when prompted, close lid, and press "OK".
4.6.5 Remove all BIs from wells.
4.7 Config Switch Test
4.7.1 Select "Config Switch" test and press "Test" or press "Next Test".
4.8 Home Sensor Test
4.8.1 Select "Home Sensor" test and press "Test" or press "Next Test".
4.9 E2ROM Read/Write Test
4.9.1 Select " E2ROM Read/Write" test and press "Test" or press "Next Test".
4.10 Servo Reading Test
Note: This test uses "Fluorescent Standards, Model 290", part number 78-8078-8421-4
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Page 14 of 38 3M©2009 78-8078-9168-0
4.10.1 Select "Servo Reading" test and press "Test" or press "Next Test".
4.10.2 Insert Low Standard into Well #12 when prompted, close lid, and Press "OK".
4.10.3 Insert High Standard into Well #12 when prompted, close lid, and Press "OK".
4.10.4 Verify completion of test in status display box.
4.10.5 Remove fluorescent standard from unit.
4.11 Motor Movement Test
4.11.1 Select "Motor Movement" test and press "Test" or press "Next Test".
4.12 Motor Slip Test
4.12.1 Select "Motor Slip" test and press "Test" or press "Next Test".
4.13 Fluorescent Standards Verification
4.13.1 Press "View Report" icon on toolbar.

4.13.2 Verify Low Fluorescent Standard Std Dev is less than 2.
4.13.3 Verify High Fluorescent Standard Std Dev is less than 4.
4.13.4 Verify High Fluorescent Standard Average is less than 250.
4.13.5 Verify difference between High and Low averages is greater than 110.
4.13.6 Close Report.
5.0 CALIBRATION PROCEDURE:
5.1 Temperature Set Point Verification
Use a traceable temperature measurement system and appropriate temperature probe that
makes good contact with incubator block walls to verify temperature in Wells #6 and #12
are within the range including:
Model 290/293 59.0ºC – 61.0ºC
Model 290G/293G 36.0ºC – 38.0ºC
Measure temperatures for an appropriate amount of time after temperature has stabilized
to ensure temperature remains within specification.
NOTE: Recommended measurement system or, similar: Fluke 77 Multi-meter and
Fluke 80K Thermocouple Module.
Temperature probe assembly, 3M stock number 78-8078-8982-5
5.2 Fluorescent Standards Verification
Fluorescent standards (78-8078-8421-4) intensity should be verified every 2 year (24

months) according to the procedure outlined on "Calibration Procedure for Fluorescent
Standards", drawing number 78-8078-9589-7.
6.0 PRESHIPMENT CHECKS/PACKOUT PROCEDURE:
6.1 Filled out and signed Machine Check-sheet
6.2 Labeling
All labeling (serial plate, warning, instructions etc.) to be legible. All labeling to be installed
per print requirements. (Adhered to unit or placed inside of box etc.)
6.3 Preparation for Shipping

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6.3.1 Place rotary switch to appropriate position based on the BI label present on the
unit according to the following table. Apply Power and verify the correct setup
mode is displayed on 7-segment display according to the following table.
Setup Mode Rotary Switch 7-Segment Power-up

Position Display
1291 BIs, Blue Label 1 F1
1292 BIs, Brown Label 3 F3
1291/1292 BIs, Blue/Brown Label 7 F7
1294 BIs, Green Label d Fd
6.3.2 With unit unplugged, insert a small flat blade screwdriver tip (Apex 00 or
equivalent) into the center split pin of the power jack on the NMOM PCB.
Spreading this split pin will ensure a snug fit of the power plug upon receipt by
customer.
6.3.3 Clean the machine of all smudges, fingerprints or water.
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Page 16 of 38 3M©2009 78-8078-9168-0
290/290G Acceptance Check Sheet page 1 of 2
Approvals:
_______________________ _______
Service Date
Serial Number _______________________
Model Info (Check Appropriate)
70-2007-2980-7 290 Attest Auto-reader 100VAC

70-2007-3170-4 290G Attest Auto-reader 100VAC
In process test Test Results

characteristics pass/fail
1) General
Workmanship
2) Ribbon Cable
3) Heater Blanket
4) Home Sensor
Adjustment
5) Sled Spacing
Across Bock
6) Error Code Test
7) Display Verification
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3M©2009 78-8078-9168-0 Page 17 of 38
In process test Test Results

characteristics pass/fail
8) Fluorescent
Standards
Low Standard StdDev <2
High Standard StdDev <4
High Standard Avg. <250
Avgerage Difference >110?

Low to High Std
9) Temperature
Verification
10) Documentation
11) Labeling
12) Workmanship
13) Rotary Switch
14) No Smudges,
Fingerprints, etc.
15) Final Packed per

Standard
Comments/Repair Information/New Test Results:

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Approvals:
_______________________ _______
Service Date
Serial Number _______________________
Model Info (Check Appropriate)

In process test Test Results pass/fail Test Results pass/fail Test Results pass/fail
characteristics TOP TIER CENTER TIER BOTTOM TIER
1) General
Workmanship
2) Ribbon Cable
3) Heater Blanket
4) Home Sensor
Adjustment
5) Sled Spacing
Across Bock
6) Error Code Test
7) Display Verification
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3M©2009 78-8078-9168-0 Page 19 of 38
In process test Test Results pass/fail Test Results pass/fail Test Results pass/fail
characteristics TOP TIER CENTER TIER BOTTOM TIER
8) Fluorescent
Standards
Low Standard StdDev <2
High Standard StdDev <4
High Standard Avg. <250
Avgerage Difference >110?

Low to High Std
9) Temperature
Verification
10) Documentation
11) Labeling
12) Workmanship
13) Rotary Switch
14) No Smudges,
Fingerprints, etc.
15) Final Packed per

Standard
Comments/Repair Information/New Test Results:

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Illustrated Parts List
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3M©2009 78-8078-9168-0 Page 21 of 38
290/290G ASSEMBLY
1 78-8083-0098-8 MEMBRANE PANEL OVERLAY 1

2 78-8078-8675-5 DISPLAY ASS’Y 1
3 26-1002-5214-2 SCREW, MACH,6-32 X.250, PAN, PHIL 12
4 78-8083-0207-5 ENCLOSURE ASS’Y 1
5 78-8078-8668-0 N/A FRAME ASS’Y 1
7 78-8083-0086-3 N/A BOTTOM PLATE 1
8 26-1004-2652-2 WASHER, FLAT #6 3
9 70-0700-7979-6 BUMPER,3M 6
10 26-1002-5195-3 SCR 2-56 X .188, MACH PAN ZN 2
11 78-8078-8756-3 LID SWITCH ASS’Y 1
12 26-1000-4179-2 N/A NUT, MACH, 2-56, ZN 2
13 78-8083-0195-2 N/A LID SENSOR BRACKET 1
14 78-8078-0402-2 N/A LABEL-SERVICE CENTER 1
15 78-8083-0087-7 COVER, BACK 1
16 26-1012-1254-1 N/A CLAMP,RICHO, CFCC-3-05A-RT 1
16A 26-1014-7689-8 N/A CLIP for FLAT CORD, WHITE 1
16B 26-1017-3696-0 CLIP for ROUNDED CORD 1
78-8078-8764-3 N/A DECAL, CAUTION 1
17 78-8078-8773-8 N/A SERIAL PLATE, AUTO READER 1
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290/290G ASSEMBLY
18 78-8078-8878-5 N/A LABEL, SHOCK/EXCLAMATION 1

19 26-1004-3301-5 SCREW, 2-56 X .125” 2
20 26-1017-0657-5 SCREW, 6-19 X .250, PAN, PHIL 1
21 26-1014-7284-8 CABLE, ELEK PARALEX 1.00 MM 1
22 78-8078-9170-6 CARD,INSERT 1
23 26-1014-7450-5 ENVELOPE, CLEAR, 2.5 X 4.0 1
26 26-1017-2681-3 N/A SCREW, MACH, 4-40 X .188, PHIL 1
N/A INDICATES PART NOT AVAILABLE
CONNECTIVITY KIT, Auto-reader, New Style: Connectivity Kit is necessary to link

the 290 Auto-readers to a PC with Merlin SW (Microsystems SPM).
1 78-8083-1166-2 Connectivity Kit

2 78-8083-1167-0 Cover-plate
3 78-8083-1211-6 Instruction Sheet
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3M©2009 78-8078-9168-0 Page 23 of 38
290/290G ASSEMBLY (OLD STYLE CASE)

1 78-8078-8772-0 N/A MEMBRANE PANEL OVERLAY 1

2 78-8078-8675-5 DISPLAY ASS’Y 1
4 78-8078-8869-4 N/A MASK,DISPLAY BOARD 2
5 78-8078-8759-7 N/A ENCLOSURE 1
6 78-8078-8668-0 N/A FRAME ASS’Y 1
7 78-8078-8761-3 N/A BOTTOM PLATE 1
8 26-1002-5216-7 SCREW, MACH, 6-32 X .375, PAN PHIL 12
9 70-0700-7979-6 BUMPER,3M 6
10 26-1002-5206-8 SCR 4-40 X .375, MACH PAN ZNC 12
11 78-8078-8756-3 LID SWITCH ASS’Y 1
12 26-1003-4052-5 N/A NUT, MACH, 4-40, KEPS 4
13 78-8078-8760-5 N/A LID SENSOR BRACKET 1
14 78-8078-0402-2 N/A LABEL-SERVICE CENTER 1
15 78-8078-8880-1 COVER, BACK 1
16 78-8078-8764-3 N/A DECAL, CAUTION 1
18 78-8078-8773-8 N/A SERIAL PLATE, AUTO READER 1
19 78-8078-8878-5 N/A LABEL, SHOCK/EXCLAMATION 1
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290/290G ASSEMBLY (OLD STYLE CASE)

20 26-1002-5204-3 SCREW, MACH, 4-40 X .250, PAN, PHIL 4

22 78-8078-9170-6 CARD,INSERT 1
23 26-1014-7450-5 ENVELOPE, CLEAR, 2.5 X 4.0 1
24 26-1014-7495-0 N/A WASHER,ASM CO., 2
26 78-8125-7843-9 BI ID LABEL KIT 1291, 1292, 1
& 1291/1292 (NOT SHOWN)
27 78-8078-8978-3 BI ID LABEL 1294 (NOT SHOWN) 1
CONNECTIVITY KIT, Auto-reader, Old Style: Connectivity Kit is necessary to link the
290 Auto-readers to a PC with Merlin SW (Microsystems SPM).
1 78-8083-1168-8 Connectivity Kit

2 78-8083-1169-6 Cover-plate
3 78-8083-1211-6 Instruction Sheet
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293/293G ASSEMBLY
1 78-8078-8668-0 N/A FRAME ASS’Y 3

2 78-8083-0380-0 LABELS, BI WELLS 1294 3
2A 78-8083-0381-8 LABELS, BI WELLS 1292 3
3 78-8078-8772-0 MEMBRANE PANEL OVERLAY 3
6 78-8083-0306-5 END CAPS, PAIR 1
7 78-8083-0307-3 PLATE, BACK 1
8 26-1002-5226-6 SCREW,MACH,8-32 X.375, PAN, PHIL 10
9 26-1017-1620-2 SCREW,MACH,8-32 X.5,PAN, PHIL, BLK14
10 78-8083-0303-2 CASE 1
11 78-8083-0396-6 DECAL, FRONT 1
12 78-8083-0305-7 BRACKETS, PAIR 1
13 78-8083-0304-0 BASE PLATE 1
14 78-8083-0392-5 POWER CABLE ASS’Y 1
15 70-0700-7979-6 BUMPER,3M 6
19 78-8083-0395-8 CONVERTER, SERIAL TO USB 3
20 78-8083-0394-1 USB HUB; 1
21 26-1007-4847-9 TAPE, DBL COATED, 1’W 3M VHB AR
22 78-8083-0391-7 CABLE, FLAT, RS232 3
________________________________________________________________________
Page 26 of 38 3M©2009 78-8078-9168-0
293/293G ASSEMBLY
23 78-8078-8869-4 N/A MASK,DISPLAY BOARD 6

25 78-8078-8675-5 DISPLAY ASS’Y 3
26 78-8083-0418-8 SPACER, THERMAL INSUL 3
29 78-8083-0437-8 WIRE HARNESS, DISPLAY 1
30 26-1017-2068-3 USB PANEL MOUNT JACK 1
31 26-1017-2787-8 DUST COVER, USB 1
33 26-1003-4052-5 NUT, 4-40, KEPS 2
34 26-1009-7475-2 TIE WRAP, #10 MT HOLE 1
________________________________________________________________________
3M©2009 78-8078-9168-0 Page 27 of 38
FRAME ASSEMBLY

1 78-8078-8389-3 NMOM ASSY’Y 1
2 26-1002-5218-3 SCREW, MACH, 6-32 X .500, PAN, PHIL 5
3 26-1014-7498-4 N/A SPACER,ASM CO, 3031R-F-.275,.250 3
4 26-1002-5216-7 SCREW,MACH,6-32 X .375, PAN, PHIL 2
5 78-8078-8386-0 N/A FRAME 1
6 26-1011-8385-8 SCR, M3 X .5 X 5.0,SKT,HEX SKT 4
7 78-8078-8758-9 N/A SLED LOCATOR 1
8 26-1002-5204-3 SCREW,MACH,4-40 X .250,PAN, PHIL, 2
9 26-100508084-9 N/A NUT, MACH,4-40, KEPS 4
10 78-8078-8717-5 STEPPER MOTOR 1
11 26-1004-7298-8 N/A PULLEY, BERG MFG, TP7A5MW2-15, 1
12 26-1014-7496-6 N/A WASHER, ASM CO., 99B-.062-D, FLAT, 2
13 78-8078-8725-8 N/A SPACER, INCUBATOR BLOCK 1
14 78-8078-8766-2 INCUBATOR BLOCK ASS’Y 1
15 26-1014-7300-2 N/A SPACER,ASM CO., 3031R-D-.312, .250 2
16 78-8078-8667-2 N/A SLED HOLDER ASS’Y 1
________________________________________________________________________
Page 28 of 38 3M©2009 78-8078-9168-0
290 FRAME ASSEMBLY

17 78-8078-8714-2 N/A IDLER PULLY SPRIG 1

18 78-8078-8716-7 N/A IDLER PULLEY 1
19 26-1014-7125-3 N/A RETAINER RING, WALDES TRUARC, 2
20 78-8078-8715-9 N/A SHAFT, IDLER PULLY 1
21 78-8656-4008-6 N/A RETAINING RING, TRUARC,5133-18-H 4
22 78-8078-8703-5 N/A GUIDE ROD 2
23 26-1014-7301-0 BELT, TIMING, B.C. OF A.,MXL270T80 1
24 78-8078-8905-6 N/A PLATE, CAP 1
________________________________________________________________________
3M©2009 78-8078-9168-0 Page 29 of 38
SLED ASSEMBLY
1 78-8078-8211-9 N/A SLED ASSEMBLY 1
2 78-8000-7655-2 SCR, 2-56 X 1.0” 1
3 26-1014-7302-8 N/A O-RING 2
4 78-8078-8670-6 N/A SIDE COVER, LEFT 1
5 78-8078-8671-4 N/A GLASS EXCITATION FILTER 2
6 78-8078-8674-8 N/A MASK 1
7 78-8078-8312-5 N/A REFLECTOR 1
8 78-8078-8669-8 N/A SIDE COVER, RIGHT 1
9 26-1000-4179-2 N/A NUT, 2-56 X 1
10 78-8078-8673-0 N/A GLASS EMISSION FILTER 1
11 78-8078-8672-2 N/A GELATIN EMISSION FILTER 1
12 26-1017-2781-1 LAMP 1
12A 26-1014-7272-3 LAMP, ALTERNATE 0
13 26-1004-3301-5 SCR.2-56 X .125” 2
________________________________________________________________________
Page 30 of 38 3M©2009 78-8078-9168-0
SLED HOLDER ASSEMBLY

1 78-8078-8704-3 N/A GUIDE PLATE 1

2 26-1014-7294-7 N/A BEARING, OILITE,AA-309-4 4
3 26-1014-7295-4 N/A SPACER, ASM CO., 14030-H-A-.875-3 2
4 26-1014-9893-4 CABLE, FLEX 1
5 78-8078-8707-6 N/A BELT TENSIONER PIN 1
6 78-8078-8706-8 N/A BELT LOCKING PIN 1
7 26-1011-5909-8 SCR, SET, 2-56 X .125” 1
8 78-8078-8705-0 N/A PCB CLAMP 1
9 78-8078-8710-0 N/A STRAIN RELIEF 1
10 78-8078-8666-4 SLED ASSEMBLY 1
11 26-1002-5206-8 SCR, 4-40 X 3/8 MACH PAN PHIL ZNC 4
12 26-1014-7293-9 SCR, SET, 4-40 X .375” 1
13 26-1014-7296-2 N/A SPACER, ASM CO., 14030-H-A-1.125-3 2
________________________________________________________________________
3M©2009 78-8078-9168-0 Page 31 of 38
3M Attest Model 290/290G Theory of Operation
Theory of Operation
This section will describe mechanical, electrical and software component aspects of the 3M Attest
290/290G Auto-reader/Incubator.
A. Electro-Optics Apparatus
The sled board contains the electro-optics apparatus for the 3M Attest 290/290G Auto-reader. Inside
the enclosed extrusion are two photo sensors and a bulb, or excitation source. Light from the excitation
source is shown on and reflected off the Biological Indicator, filtered to the desired wavelength, and a
proprietary embedded control system accurately measures the fluorescent activity. The embedded
control system also monitors the bulb intensity. This apparatus should not be disassembled unless the
manufacturing guidelines are followed.
B. Sweep Measurement Timing

The measurements of relative fluorescence for each biological indicator are internally timed by a
micro-controller to take place at the same ‘on’ time for all readings. The fluorescent measurement
time and the amount of time to transport the elecro-optics to another biological indicator well is 2
seconds. See Table 1 below.
Reader/ Order Time after bulb turns

incubator Well # Read ‘on’ to fluorescence
measured
12 1st T0
11 2nd T0+2
10 3rd T0+4
9 4th T0+6
8 5th T0+8
7 6th T0+10
6 7th T0+12
5 8th T0+14
4 9th T0+16
3 10th T0+18
2 11th T0+20
1 12th T0+22
Table 1
Since the excitation source’s output intensity varies with respect to time ‘on’ and the proprietary
embedded control system’s ability to minimize the output variations, this timing technique assures that
each biological indicator placed into a reader incubator well will be read in the same excitation source
‘on’ time with a variation of less than +/- 1% of the total specified readout time.
C. Measuring Fluorescent Intensity Increases.

Design considerations for the Electro-Optics apparatus were made on the assumption that the intensity
increase of fluorescence would be measured. Since background fluorescence can vary between BI
vials, this measurement of fluorescent intensity increase eliminates the requirement for BI’s to have
similar background fluorescent measurements. Furthermore, since the detection of positive and
negative BI’s will be based on the change in fluorescence during incubation instead of fixed threshold
values, this measurement method eliminates the need for daily calibration.
D. Excitation Intensity
Each time a biological indicator is read by the electro-optics apparatus, the excitation source will
remain on for a fixed length of time, regardless of the number or location of the biological indicators.
________________________________________________________________________
Page 32 of 38 3M©2009 78-8078-9168-0
After this on time, the excitation source intensity will be measured. This technique will allow the
ability to measure the output intensity of the excitation source after it has been operated, and stabilized.
If this output intensity is less than a chosen set-point, then the excitation source failure determination
can be ascertained. Error message (E8) will be displayed for the user if the excitation source is too dim
to accurately measure fluorescent intensity.
E. Sled Positioning.
The Electro-Optics Apparatus is mounted on a moveable platform called a Sled. The Sled position is
referenced to a home position via a sensor mounted on the sled and a vane mounted on the frame. This
establishes the home position of the sled. The home position is determined automatically at power-up
as well as when a series of BI readings are completed. Positioning is accomplished with a stepper
motor as an open loop system. The motor is given a certain number of steps to move the Sled from
one well position to the next. The number of steps moved away from the home position is
accumulated, and when the Sled returns home, the number of steps needed to move home is subtracted
from the accumulated value. A difference of more than 3 steps between the two values 3 consecutive
times will result in the system preventing normal operation, and an error message (E9) will be
displayed for the user. If the difference is more than 3 steps, the readings will be ignored and retaken.
F. BI Insertion Detection.
BI presence in each Incubator Block Well is determined using a photoelectric sensor mounted on the
NMOM board and placed next to each well. When the unit detects the cover open, the BI detection
sensors are read and the A/D value is compared to a setpoint. A value above the setpoint indicates the
presence of a BI. A value below the setpoint indicates an empty well.
G. Incubator Block Temperature Control.

Incubator Block temperature is maintained by a heater blanket mounted on the Incubator Block. A
thermistor on the heater blanket is measured with an A/D converter and the value is compared to a
setpoint. If the value is at least one count above the setpoint, the heater is turned off. If the value is at
least one count below the setpoint, the heater is turned on. The setpoint is saved in the EEPROM. The
value is automatically changed via the rotary setup switch for 60°C or 37°C operation. When the unit
is first turned on, a warm-up caution code (C1) is displayed and the unit is non-functional until the
setpoint is reached. During normal operation, a high or low temperature condition will result in the
system preventing normal operation, and an error message (E1) will be displayed for the user.
H. Display.
The display consists of red, yellow, and green LEDs for each Incubator Well and dual 7-segment
displays. When a BI is inserted into a well, the yellow LED will light, indicating a BI in process.
After the full incubation time, if the BI result is negative, the green LED will light and remain on until
the BI is removed. If at any time during incubation a positive result is determined, the red LED will
light and remain on until the BI is removed. The buzzer will also sound if enabled, until the "buzzer"
button is pressed or the BI is removed. If a positive BI is removed then red LED will remain lit for
approximately 10 seconds, allowing the BI to be replaced for growth readout purposes. The 7-segment
display will display caution and error codes as well as time remaining for each BI if the "time
remaining" button is pressed. The LEDs all light in a test mode when the cover is opened. If a BI is
removed before incubation is complete, all LEDs for that well will light and the buzzer will sound for
10 seconds, with caution code C2 displayed, indicating the need to replace the BI. After 10 seconds,
the BI data is lost.
________________________________________________________________________
3M©2009 78-8078-9168-0 Page 33 of 38
POWER
JUMPER INSTALLED FOR TEST PU J12
POWER.SCH
NCOM 1 1
V+
+8V NCOM.SCH 6 6
+8V NMOM
J1 2 2
TX TXD
1 2 SLED 7 7
RX
J4 LO 3 3 PC HOST
V- LO RXD
-10V DGND PGND J3 FPC18 +12V VDD CONY 8 8
-10V CONY
JUMPER2X1 +12V 4 4
1 18
+12V 9 9
2 17 L1
SENSOR EC VDD +8V +8V NEC ASYNC SERIAL INTERFACE EIA-232D 5 5 COUNTRY SPECIFIC PIN AD
3 16
SENSOR.SCH EC.SCH -10V -10V NEC.SCH ELDR-35
4 15
DGND SUBD9PCST
V+ EMRST* EMRST* HOME 5 14 SLEDHOME TX
SLED INTERFACE
V+ EMINT* EMINT* 6 13 RX
CONY NPOWER
EXRST* EXRST* RST* 7 12 RST* CONY
NPOWER.SCH J2 EXTERNAL POWER SUPPLY
V- EXINT* EXINT* 8 11 STAR GROUND POINT
LO LINE
V- SLEDCS* 9 10 SLEDCS* LO +PS
J8 GLOBAL COMPATIBLE SWITCH MODE P
VEM VEM 10 9 HI HI
1 2 INPUT 90 < VAC < 264 , 47 < HZ < 62 NEUTRAL
VEX VEX SDO 11 8 SDI
OUTPUT 12VDC , 30W REGULATED
12 7
DGND PGND PC722A EARTH
SDI 13 6 SDO
+8V JUMPER DRC TRACE
14 5 +8V
-10V
SCK 15 4 SCK -10V
EXCITE J1 OPTIONAL INCUBATION TEC
16 3
EXCITE.SCH
PINGMOM* 17 2 PINGMOMS* 1 1
LAMP LAMP 18 1 TECA 2 2
INC* INC* TECB 3 3
FPC FPC CABLE MELCOR CP1.0-63-08L
U/D* U/D* DGND 4 4
DGND
PTCS* PTCS* HEATH HEATH
PGND PGND MTE4
LAMPV LAMPV HEATL HEATL
+12V J7 INCUBATION HEATER/THERMISTOR
+12V COOL COOL
HOMELED* HOMELED* HRTHI 1 1
VDD HEATER HI
2 2
3 3
NT T
4 4
+12V
5 5 THERMISTOR
6 6
7 7
HRTLO 8 8 HEATER LO
FPC8 COPPER ETCH ON KAPTON

DISPLAY
DEC VDD +12V +12V VDD NT
NTC
DEC.SCH J1 J5
RST* 1 1 RST*
J2
2 2
DISPLAY INTERFACE
1 SW1* DICS* 3 3 DICS*

NBI
2 SW2* 4 4
NBI.SCH
3 SW3* SDO 5 5 SDI
4 SW4* 6 6 SCK SCK
5 SDI 7 7 SDO SDO SDO
8 8 PUR* PUR*
FFC5 DGND
SCK 9 9 SCK OEXPCS* OEXPCS*
10 10 LEDEN* LEDEN*
MEMBRANE INTERFACE
PINGMOMD* 11 11 PINGMOMD* PUR* PUR*
12 12 BIHOMEA BIHOMEA
BIHOMEB BIHOMEB
FPC12
FFC12 FPC CABLE BIHOMEC BIHOMEC I0
DGND DGND M1
BIHOMED BIHOMED I1
DPOWER
PGND PGND
DPOWER.SCH NMOTOR PHASE A1
NMOTOR.SCH
ASEG ASEG
BILED
BSEG BSEG I0
BILED.SCH J6 J6
CSEG CSEG VR VR I1
BLACK
LEDEN1* LEDEN1* DSEG DSEG MPHA1 1 1
LEDEN2* LEDEN2* ESEG ESEG MPHA2 2 2
BROWN PHASE A2
LEDEN3* LEDEN3* FSEG FSEG PHA PHA MPHB1 3 3
YELLOW PHASE B1 MTR STEP 2PH 4W 17FRAME
LEDEN4* LEDEN4* GSEG GSEG PHB PHB MPHB2 4 4
LEDEN5* LEDEN5* DPSEG DPSEG 5 5
ORANGE PHASE B2
LSCK LSCK MSD MSD 6 6
LSDO LSDO LSD LSD JAPAN SERVO KH42HM2-801
MTE6
STORE STORE BUZ BUZ AMP MTE
VLEDEN VLEDEN
VLED VLED
________________________________________________________________________
Page 34 of 38 3M©2009 78-8078-9168-0
Certificate of Calibration
Serial Number: _____________________
Model Number: ____________________ 290 Auto-reader ____________________ 290G Auto-reader
The serial number listed above has been tested and found to comply with 3M’s manufacturing
specifications applicable to subject device at time of manufacture. It has been tested with NIST
traceable instrumentation to verify it meets the following specifications.
3M Test Procedure Number: C-05-005002
1) Temperature Specification:
290 60ºC +/-2ºC
290G 37ºC +/-2ºC
Well #6 Measurement ºC
2) Relative Fluorescence Measurement Increase Specification:

Greater Than 110 Relative Fluorescence Units
Measurement Units
3) Fluorescence Measurement Standard Deviation Specification:

Less than 4 Relative Fluorescence Units
Measurement Units
Test Equipment:
Equipment Used (Temperature):
Equipment ID:
Calibration Date:
Calibration Due:
Equipment Used (Fluorescence): Fluorescent Standards

Equipment ID: 78-8078-8421-4
Calibration Date:
Calibration Due:
For factory calibration and re-certification of this equipment contact:
3M Health Care Service Center

Suite 200, Bldg. 502
3350 Granada Avenue North
Oakdale, MN 55128
1-800-292-6298
Fax: 1-800-770-8016
Outside of the USA, contact your local 3M Subsidiary.
Calibrated By: _________________________________ Calibration Date: _________________________________
QC: _________________________________ Date: _________________________________

Certificate of Calibration
Serial Number: _____________________
Model Number: ____________________ 293 Auto-reader ____________________ 293G Auto-reader
The serial number listed above has been tested and found to comply with 3M’s manufacturing
specifications applicable to subject device at time of manufacture. It has been tested with NIST
traceable instrumentation to verify it meets the following specifications.
3M Test Procedure Number: C-05-005002
1) Temperature Specification: 293 60ºC +/-2ºC, 293G 37ºC +/-2ºC
2) Relative Fluorescence Measurement Increase Specification: Greater Than 110 Relative Fluorescence Units
Top Tier Measurement Units

Center Tier Measurement Units
Bottom Tier Measurement Units
3) Fluorescence Measurement Standard Deviation Specification: Less than 4 Relative Fluorescence Units
Top Tier Measurement Units

Center Tier Measurement Units
Bottom Tier Measurement Units
Test Equipment:
Equipment Used (Temperature): _______________________
Equipment ID: _______________________
Calibration Date: _______________________
Calibration Due: _______________________
Equipment Used (Fluorescence): Fluorescent Standards

Equipment ID: 78-8078-8421-4
Calibration Date:
Calibration Due:
For factory calibration and re-certification of this equipment contact:
3M Health Care Service Center

Suite 200, Bldg. 502
3350 Granada Avenue North
Oakdale, MN 55128
1-800-292-6298
Fax: 1-800-770-8016
Outside of the USA, contact your local 3M Subsidiary.
Calibrated By: _________________________________ Calibration Date: _________________________________
QC: _________________________________ Date: _________________________________

3M Health Care Recycled paper
3M Center 40% pre-consumer
Building 275-4E-01 3M Health Care 10% post-consumer
St. Paul, MN 55144-1000 D-41453 Neuss, Germany Litho in U.S.A. with 3M film,
1-800-228-3957 proofing systems and offset
plates.
Attest is a registered trademark
of 3M.
© 3M 2009
78-8078-9168-0. Rev. D

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Revision Date Page(s) Changed

Change: Birth Notice, Manual Released

B 7/16/2003 Deleted Pages 5-13

Change: Eliminated Operator’s Manual Section

ACCEPTANCE TEST PROCEDURE

ILLUSTRATED PARTS LIST

Please refer to Operator’s Manuals for Normal Operation:

Code Problem Solution

C1 Unit Warm-up Caution Allow a 30 minute warm-up after

E1 Temperature Control Error Check heater blanket connection

E3 Memory Error. Replace NMOM PCB

E4 Analog-Digital Conversion Error. Replace Sled PCB

E7 Communications Error Check ribbon cable connection between

E8 Bulb Error Check for NMOM Software V1.2

E9 Transport Error Check for loose set screw on sled

Trouble Shooting Guide (Continued)

Other Problem Solution

Unwanted alarm sounds when Turn alarm off, refer to features

Negative Control Vial

Vial not crushed. Crush vial before inserting into well.

Control vial was sterilized. Use a non-sterilized vial as a control.

Adhesive residue on vial. Do not place tape or labels on vial.

Inconsistent Read Time

Intermittent Power Connection Spread split pin on NMOM power jack to

ACCEPTANCE TEST PROCEDURE

1.0 DESCRIPTION: Attest Auto-reader Final Acceptance Specification and Packout

1.1 This procedure covers the following Auto-readers:

Finished Goods # Description

1.1.1 70-2007-2980-7 290 Attest Auto-reader 100VAC

2.0 SPECIAL INSTRUCTIONS:

2.2 Standards and specifications given on assembly and component

2.3 Hardware fasteners must be fully seated and tight.

2.6 Switches must operate without binding.

3.0 ASSEMBLY/TEST PROCEDURE:

3.1 In-Process tests

3.1.1 General Workmanship

3.1.1.1 Screw tightness, solder joint quality, wire connections etc

3.1.2 Ribbon Cable Assembly

3.1.3 Heater Blanket Application

3.1.4 Home Sensor Adjustment

3.1.4.1 Remove outer case.

3.1.5 Sled Spacing Across Incubator Block

STEPS TO REMOVE COVER OF 293/293G AUTO-READER:

1. Remove 4 screws from back cover

2. Remove 8 screws used to mount the top cover

USB to RS232 ADAPTER

Proper Repairs Prior to Shipping the 293/293G

293/293G Frame Top View

Auto-reader Display Ribbon Cable Disconnection Resolution.

- Proper Repairs Prior to Shipping the 293/293G includes:

1 78-8083-1005-2 3M 8422 Polyester Ribbon Connector Tape 3

- For Shipping 293/293G:

4.1 Error Code Test:

4.1.1 Disconnect power and remove cover.

4.2 Display Verification:

4.2.1 Set rotary switch to position 1.

4.3 Automated Test System Setup, Manufacturing Test System (MTS)

4.3.1 Set rotary switch to position 0.

Note: If any tests fail, re-test once before re-working unit.

4.4 Serial Communications

4.4.1 Select "Serial Communication" test and press "Test"

4.5 Lid Test (290/290G Only)