Certificate Acknowledgement Introduction Objective Theory Procedure Observation Conclusion Introduction
Every health book insists on the chewing of food.
The act of chewing stimulates the excretion of saliva. Saliva mixes up with the food and helps its digestion. That is, the enzyme ptyalin or amylase present in human saliva hydrolyses the big molecules of food into many molecules. For example, starch into mono-saccharides maltose and glucose; proteins into amino acids and fats into fatty acids and glycerol. Thus, saliva not only helps in digestion of food but convert it into energy generating substances. Further, enzymes and their activity are very sensitive to temperature and pH. Even a slight variation in these two factors, can disrupt the action of enzymes. In other words, digestion of food by salivary amylase is also affected by pH and temperature and can be verified experimentally. For example, hydrolysis of starch can be verified by testing it with iodine solution. Starch forms blue colored complex with iodine. If no starch is present in a system it will not give blue color with iodine In the Beginning
The digestion of the food starts as
soon as we put food in our mouth. Our teeth cut the food into small pieces and the salivary glands secrete saliva that mixes with these food materials. The saliva contains an enzyme called salivary amylase which hydrolyses starch into maltose. The complete digestion of starch occurs only in the small intestine by the action of pancreatic. EFFECT OF TEMPERATURE All enzymes are proteinaceous in nature. At a lower temperature, the enzyme salivary amylase is deactivated and at the higher temperature, the enzyme is desaturated. Therefore, more time will be taken by an enzyme to digest the starch at lower and higher temperatures. Optimum temperature for the enzymatic activity of salivary amylase ranges from 32 °C to 37 °C. The optimum temperature means that the temperature at which the enzyme shows the maximum activity. At this optimum temperature, the enzyme is most active and hence, takes less time to digest the starch EFFECT OF PH The optimum pH for the enzymatic activity of salivary amylase ranges from 6 to 7. Above and below this range, the reaction rate reduces as enzymes get desaturated. The enzyme salivary amylase is most active at pH 6.8. Our stomach has high level of acidity which causes the salivary amylase to denature and change its shape. So, the salivary amylase does not function once it enters the stomach. Objectives of the Project Report:
The main objective of this chemistry project report is “To
Study the digestion of starch by salivary amylase and effect of temperature and pH on it” and
To study digestion of starch by saliva.
To study the effect of temperature on the digestion of starch by saliva. To study the effect of pH on the salivary digestion of starch. THEORY
Every health book insists on the chewing of
food. The act of chewing stimulates the excretion of saliva. Saliva mixes up with the food and helps its digestion. That is, the enzyme ptyalin or amylases present in human saliva hydrolyses the big molecules of food into many molecules. For example, starch into mono-saccharine maltose and glucose; proteins into amino acids and fats into fatty acids and glycerol. Thus, saliva not only helps in digestion of food but convert it into energy generating substances. Further, enzymes and their activity are very sensitive to temperature and pH. Even a slight variation in these two factors, can disrupt the action of enzymes. In other words, digestion of food by salivary amylase is also affected by pH and temperature and can be verified experimentally. For example, hydrolysis of starch can be verified by testing it with iodine solution. Starch forms blue colored complex with iodine. If no starch is present in a system it will not give blue color with iodine. The requirements for experiment of PROJECT REPORT are as under: Test tubes Test tube stand One dropper Beaker Stop watch Starch and Iodine solution Thermometer Dil. HCl and Dil. NaOH solution