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—151 tay, Zonls Pe fy 1990. 44-5 L RHYTHM OF TESTICULAR ACTIVITY IN RELATION to NUA ZARD, VARANUS FLAVESCENS (HARDWicKi, AND GRAY). cIRCUM AN HAEMATOLOGY OF A MALE LI (Received May 19, 1990; Accepted November 30, 1990) Ved Prakash, Gagan K. Thakur & Braj J. Choubey* §, P. College, Dumka (S. ¥:) Bihar, 814 101, Tudia Department of Zoology, Bhagalpur University, Bhagalpur, India Postgraduate Department of Zonlogy, Studies on circum annual rhythm of testicular activity in relation to haematology were inade ona lizard, Varanus flovescens, The testicular activity and haematological parameters showed parallelism with humidity and rainfall was found to be most effective. Environmental factors such as temperature, humidity, and raintall have beet reported to play an important role in reproduction of reptiles". In recent yeatt certain reports are alsy available on the seasonal changes In the blood components @ some Indian reptiles*..However, the information regarding the role of testicula ‘activity .on haomatology of reptiles are rare"-*. The aim uf present. Investigation} to know the effect of vatious environmental factors (temperature, photoperio! rainfall and humidity) on the cyclic activity of testis and blood parameters ¢ carnivorous Indian lizard, V. flavescens. MATERIALS AND METHODS Live adult healthy male specimens of V. flavescens of almost same length af weiaht were procured from local dealer at Bhagalpur during different months of? Haematology of male lizad [145 year. They were acclimatized in laboratory conditions for a week. The animals were sacrificed on 15th day every month. Specimens were first anaasthatizad and weighed and measured from snout to vent and vent to tail tip. Pentabarbitone (U.1 mi/1009 body weight) was used as enaesthesia. Animals were dissected and testis removed and cleaned properly in saline water. After proper soaking of water with “filter paper, testis were weighed on electro-monopan balance and fixed in Bouin's fluid, Sections wore cut at & to7 microns and stained witl laeiutuaylin and Cosin. The blood samples were collected in a citrated syringe by resorting to cardiac puncture. Erylluucyle tolal counts were made by Naubaurer’s improved haemocytometer using haem's diluting fluid", Haemoglobin concentration was determined by Sahli's haemoglobinometer, Haematocrit was measured by micra~ haematocrit method using micro-haematocrit reader. Meteriological data of day-length, humidity, rainfall and temperature wore procured from Bhagalpur Agricultural College, Sabour. OBSERVATIONS The testis weight. relative weiaht and histology of the testis showed cyclic changes in different months of the year. The testicular cycle of this animal can be divided into four phases such as progressive, peak, regressive and inactive phase. The maximum relative weight of the testis was noticed in the month of July/ ‘Auoust and minimum in the month of December/January. From the month of February itstarted Increasing in weight which ultimately reached to maximum in the month of July/August Table 1). Testis showed progressive condition in the month of May/June, the spermato- gonia were seen in dividing condition and lumen were becoming narrower. In the month of June traces of immature spermatozoa were also noticed in the lumen mostly in peripheral region (Figo. 1, 2.)- Prakash, Thakur & Choubey 146 J evo ooo 8100 700079 oo er “ore Fes = FLES"S =F 490.0 FOURS OL — srSE 8G OL “90g +90 #90°0 900'0 9900" 98ST Fos'9e Fss-9 FSI90I Fa30°0 Foss Ol — (S16 gaz Ltt “AON S80 +900 1190 B00'0 Iv9T Foals, Fes'9 FLL OL 8010 Fseooe 1 C'S 9G ZOE «IZ PO 1L'0 100 900°0 200°0 L601 F00°82, Ore Feu Fu Fsezz9 2 s'sil gi16 §=6ze = 9'sz_— “des 90 160°0 9090 so0'o wr Fos‘0g Fore Fserll = Fz780 FS7S69 Zl E'1bs 1816 ree '9T “Bn seo S880 +000 Soo’ Ss'pT Feuse -FIS8 FL Fs1e0 ~—-Fz¥e9 er 682 616 GIs 6s “inp we 7900 909°0 scovo 89°01 Foose F5e8 Fesir Foo ‘Feu'ooL 61 BISs E68 Lz gz _‘unp $80 +90°0 6090 scor0 sri Fee F564 FTW | Fsse'0 Fsslt9 $I BOOl ESPs OSE Sez - AeW oD s80°0 S100 C00°0 00°01 Fetes FL9'9 Fore Foro Fsviso 31 SO OBL 85E SIE uy. Tr 079°0 900°0 7600 LOBEL Fore F's Faye Fee | FONSI 6S IS ete g's) ew $80 190°0 8100 coo “oe Felt Fors «Fees = Fromro0 | Fareeg swe osg zor ‘494 8r0 190°0 +290 70000 89°11 Foc ea, Fore F9rg F020'0 Feceg Ol 8% ree pez org -uep 6901/8 ‘sy ww = su ww yo uy % % 01/6 A001 S4s0} “Buy vey “ep Kup xey wi Ada aH day _esnvied m Apog Keg wey wn ainyereduies” sthuoyy Ca ST9=u) arok yo sypwour 3ud39y; wrqo|SomoEy ‘5994209 £49 JeI03 ‘S]9529 30 “am 4poq aBexoae ‘qSuaj Sep oSexons “1y; KEP UE Suasr22e)f snuviog ‘ayems Jo spx203¥mIOEY puL 3M saReped ‘a syy891 aanjosqe weoux ‘qiSua |

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