| in ., Vol. 4, 1991 Hin pfny, etn Pol TO Hi jprgcTs OF METHYL TESTOSTERONE AND TESTO: ISTERONE PROPI pROTEIN, GLYCOGEN AND MOISTURE CONTENTS IN LIVER AND aa “ A FRESHWATER TELEOST, HETERUPNEUSTES FOSSILIS fae OF (Received August 16, 1990; Accepted December 5, 1990) R. Lal and 8, 8, Srivastava Department of Zuvlugy, T. D. College, Jaunpur-222 002, India ‘Adult male and female Heterspneustes fossilis’ were injected with different doses (1.25, 2.50 and 5,00 #g/g body wt.) ‘of methyl testosterone (MT) and testosterone propionate (TP) on alternate days till each fish eceived a total of four injections. Protein, glycogen and moisture contents were determined in liver and gonad, Protein and glycogen values of liver and yonad were significantly increased io oth sexes in response to MT and TP, These changes were dose dependent, Moisture percentage aid not change significantly jn both the tissues following the steroids treatment, Careful statistical comparison of MT and TP showed that Mr was more effective in inducing such changes in both the tissues at all dose levels tested. gonad metabolism of fish has vident from earlier otudios that of fish are subjected to jations in external steroids) eases vitellogenic ns, The inadequate x steroids need The involvement of sex steroids i liver and tilracted the attention of many invastiaators. = of the biochemical constituen wtked seasonal changes which sanveratue, phetogeriod oallnllaetaeer clos, In teleosts, exogenous aamninictaiO a weaty in fiver’, plasma protein level mations regarding the responsivene:Lal & Srivastava 459] ermine the effect of methy! is to det By emical constituents of liver eer intention of this st further investigation. The intention suine bioch! entpeterone and testosterone propionate on and gonad of Heteropnenstes fosill, MATERIALS AND METHODS lis (weight range 38g—40g and length December from pods in the vicinity of ‘oratory conditions for three weeks Male and female specimens of H. foss range 17em—19. cm) were procured during Jaunpur city, India. They were avclimatised to lab before starting the experiments. Fishes were divided into seven groups of 10 males and 10. famaies each. They are first lightly anaesthesised with MS 222 and then groups 1, 2 and 8 were intramuscularly injected with 1.25, 2.80 and 5.00 Hy MT/g body Qe and groups 4,5 and 6 with 1.25, 2.50 and 5,00ng TP/g body wt, respectively on alternate days till each fish received a total of four injections. Last group received comparable quantity of carrier vehicle only and served as control. The fishes were fed with a compound ground mixture of goat liver, dry prawn and wheat flour ad libitum at overy altarnate days. throughout the experiments. Aquaria water temperature was not artificially controlled but ranged in between 16—18" C. Twenty four hours after fourth injection fishes of all groups were decapitated andtheir liver and gonad were taken out, washed with 0.6% saline, blotted to remave ‘excess saline, waighed and usad for tha determination of protein, glycogen and pa Protein was determined using the method of Lowry e at.’ and glycogen by Kemp and Adrienne’ as modified by Srinivas i Kris , water content was determined by drying Ge wanes tissues at fone i ca mys until constant weight were obtained. Hepatosomatic (HSI) et (GSI) indices were also calculated using the established aes 86 gonosomalc All the data were analysed statistically with the help of Stud ‘udent's RESULTS 's t-test. Results were summarised i ; in tabl a umn in les | anu 2, ic Protein values were significantly increased tetas, non ona ee ee “°°: 0.01, 0.001) in both sexes follo-[158 Effects of Steroids on Liver and Gonad of H. fossilis “100 0> clexx '10'0> dee ‘€0'0> de 124U0D WOH eouoL9y)p 40 ooUEDI}1UBIg 100°0F oer wer wor MOF SezF peer co Ose, arbG SL SET HR eS = jonuog TOOF sev eeDI= GOOF Bor cee onst ver‘ SCL weDR'CET ww S"T AGEL sestl'08 cos db TOF STF ars 100F seer eee ze0'0 OSL | wZOCT n't See. EOL we dL 200°0F eer 8 800F weve aye ze'0 Ove «= OOFFO OT woe 9s'88 seu dL 200 0F sole ost wor IF STF 980°0 181L wen0Z'Eb)en"S B6'EL anrt6'6 o's AW 40007 lozt SOF WOT SOF StF COFF seoo SZ'L — anDSSCLanDH'S TST: SITE want8'20 Deg am zoo'oF weer Bs01> Boor Loo rer LOST E00 Beth ISHIL geebI'G 08 GIL wet0'S2 ws9NB SCT iW 3SEW 3STW . 3S7N 3SFW3SFW ESTW 3STW aSTW qa yon em EM Sw yom “yay “yAyeM WM “ 5/5w 6)6u % . f/ou —6j6w——fpoq 7 eamsjow ujsjosd usbo2Kj0—O/ ujejord = ue600K {5 se) semouseL eanjsiows Iso reynoysey 2eIN: ISH ton] Jen] 9A #80q euOWOH “a ptss0f “HJ 189 PXE teoSoosy3 seynopisos {gH ‘9403910 SOAK ‘eFIOLd 3943, 059980389 [AqVeUE J° 899995 —T omeL oiicaissll eanysyous se[nONs93 ‘uyq04d -re]KOFISy oplo1d omo197503803 pur om“100'O> dx “10°0> dak ‘SC'O> dx 104}U09 WOsJ BdUEIO}}/P 4c OLED! HUBIS Lal & Srivastava 154] wor Ost oer GOOF ChOF eesF ELIF pL 690 BEL 61'96 08'0 = BgLGESL «ODD OZET = louog wor wer w+ RDF OOF OGIF HEF STIF BLO HOLL wxS'631 waa bL'T PLT SZEL an QI'F6 HOS*IE 00's dl WF TF sor 8O0F OLOF OsTF Seer OTIS wo eL apVEOL — eeeOB'T —IL'E CEL DELL =O 'ZE ose dL OF 0¢eF sree DF ZIOF OSsF g6'SF 89OF oLo 6atee 61°96 wweS"l TLE BI'9 OL LT. ser di wor ocr Berl F sooF sor ETF OSPF 11S slo FeOL ees OST 9x608-1 wh PCL seal TIT ZOOS 00's Lw Wor OTF LL vOCF 20°0F tF SOS Cy v0 ssOL 6°51 tee PST wT TStL we01S8 ISOS ose lw OOF 3°77 LOL s00F POOF OFtF ost SRIF wL'0 STL POSIT wee bS' Olt ESL tS SL ol OTE StL iw aSFN SFY BSTN = ASFW ASTIN aSFW aSFN aSFW "A yMIOM ym Jom om JOM IM Jom “mM jem wa % 6/ou s/cw % 6/8u 6/6u —poq eunysjou —urejord—uaB004\6 errysjow ujeyosd ueBook|6 ——6/B IS9_ueeND —ueLeA Ue ISH on] don] sont escq euoWwsoy, Of FIC ISD PUY samisrour uEreA0 ‘upazoad a0ngy z rae smqord mepreao ‘aaF00k{S weptwas {SH “sansyour 3944 “wodea4ia sony, we seuopdord owoxoys09803 pre owesaxs03603 [(qa2ur zo S192; 35—gtfecte of Steroids on Liver and Gonad of H. fosslis. [135 gut and TP treatments when compared with controls. Thase changes were dose vi cepondont fh the steroids tested significantly Increased glycogen contant of liver and s in respect to controls, The optimum dose iver and gonad were found to be Bot! gonad (P 0,05, 0.01, 0.001) of both sexe: lal for MT and TP to increase ylycogen value in 1 {95 and 2.50 gig body wt. respectively. All the doses of MT and TP did not significantly alter the moisture percentage stlver and gonad, HSI and GSI valuas In both soxos~ DISCUSSION Inmost of the non-mammalian vertebrates yolk proteins and its precursors are of extragonadal origin, Although hepatic synthesis of yolk protein, vitellogenin Is inflasneed by estragont’—H as well as androgens 18 4, The serum concentration of androgens were extremely nigh throughout the ‘course of vitelluyenesia, gonadal de lopment and spawning migration in the fish and at that time plasma protein level is errandings both MT ane TP enhanced the hepatic as well as increased*~”. In the presen’ qonadal protein synthesis. Medda + alt have reported that testosterone failed to Mauve eny significant change in protein content of Iver and gonad whore as estradiol Teroased protein content of livers Howover, anqoger like tstosterone may be serving ae precursor for estrogen synthesis“, Therefore, it is likely that these Ten as roported by 1aMenn ef al. Increased amount of hhormones acted via estrog Wwotein in gonad maybe due to the deposition of extragonadal or intragonadal originated proteins. In the present study both MT and TP incroaced the hanatic a6 well as gonadal both sexes suggesting that an anabolic ole of these hormonal Moddat have reported anabolic effect of testosterone in liver and ower and Florini®* in muscle ot f18nes. The role of male steroids on carbohydrate metabolism has also been demonstrated", During sitellogenesia, gonadal growth and spawning, high eneray is required for muscular a alycogen content in In H, fusitis, Similarly Dasmahapatra_andLol & Srivastava 156] in the liver and erage of glyvogen in vonag, che. rane glycogen CONtENIS OF liver a7 higher magnitude than the tp This necessiates 1 et er than TP in inducing gyep traction” ae This evident that NT induced cao bs en dat different dose levels 10S oh Soe ene imely that MT [s mare changes. orted that testosterone, cause no signitican, Tiver and gonad of H. fessilis. Our findings suppor, gs of testosterone, a slight change in mois. of anabolic activities prior to spawning, Dasmahapatra and Medda* change in moisture percentage in above view, although we have used anolo: ture was observed. This could be due to onset ACKNOWLEDGEMENIS The generous gift of MS 222 fom Sandoz Ltd., Switzerland and the financial assistance from U. 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