|. Him, J. Env. Zol., Val, #, 199 f i RELATIONSHIP BETWEEN SERUM PROTEIN AND OVARY PROTEIN | OF CHANNA PUNCTATUS (BL.) (Received September 18, 1990; Accepted November 6, 1990) Amita* & O. P. Saxena i Zoology Retearch Laboratory, M.M. College, Modinagar 201 20+, India i *Department of Aquaculture, College of Fisheries, G B, Pant University of Agriculture t & Technalngy, Pantnagar—253 145, India j i This paper deals with study of ovary protein and serum protein during ovary maturation, A reciprocal relationship has been established between these two parameters. The lowest level of ovary protein and highest level of scrum protein in spent etage euggest that serum protein, is related with maturation, The capacity of living organism for storing proteins is limited and relatively small but it furnishes certain essential component to living tissues, The prote ns are of paramount importance not only because of their specific chemical and physiv- chemical properties but also because they appear to confer upon various types of cells and their biological characteristics. Proteins in blood plasma serve a number of purposes including regulation of water and electrolyte balance, providing antibodies etc. “', The aim of present study is to find out the relationship between serum protein and ovary protein af Channa punctatus, MATERIALS AND METHODS, Fs Channa punctatus were collected from local fresh water resources of Distt. feerut_ and Ghaziabad (U.P.) round the year, For the estimation of protein the122] Amita & Saxena ovary was homogenised using chilled glass distilled water to form 1C% homogenate and processed for Biuret method as described by Oser’. For serum protein blood was taken from the hepatic portal vein just at the point where it joins the sinus veno- gus by means of a fine micropipette which was previously rinsed in a 3% sodium citrate, RESULTS AND DISCUSSIONS During stage II of the ovary there is a marked increase in energy dynamics and proteins constituents as compared to stage |, mainly due to metabolically active phase of the ovary. Higher biochemical component in developing ovary of Claris barvachus were also.observed by Gupta and doshi®. In the present case ‘conspicuous increase in the protein contents in stage III was noted, which depleted in stage IV of the ovary. The serum protein, albumin and globulin in blood vary greatly in their bio-phy- siological activities and are active in regulating various biochemical processes in living system *-*, It was observed (Table1) that during - stage | the serum protein was 12.2-+.84 mg/100 ml., it fell down to 11,8-+,86 mg/100 ml during stage II, the trend followed and serum proteins were reduced to 8.6-+ .63mg/100 ml of the blood nd increased 15.40.28 mg/100 ml_in stage IV. On the other hand the lowest level of ovary Table 1—Total protein content the ovary and blood serum of Channa punctatus. Ovarian Protein (mg/100 ml) Stage Ovary Serum ' 26.6 + 2.32 122 + 0.84 " 86.2 + 3.14 11.8 + 0.96 WwW 18.4 + 1.48 8.6 + 0.63 Bo IMac 124 1,22 15.4 + 0,28Serum Protein & Ovary Protein of Fish pene toss 0 ouny “Moree in Fig. 1—Relationship between serum protein and ovary protein in stage |. Hee genus FROTEW (123 nd Woas ait aS ROE Fig. 2—Relationship between serum protein and ovary protein in slage Il. eof. 88h ° FeCRESSION UNE o iso. ° 2 [8 a & ° us. 5 eae Bun oo eo bee REGRESSION LINE 2 ht. - EXPERIMENTAL VALUE io er CALCULATED VALUE . . 3.1957 + 0.6868x \ : nao M L . 1 SWS Was _BS_ uous 180 ‘Owny PROTEW fy) Fig. 3—Relationship between serum protein and ovary protein in stage 111. ‘ovary PROTEIN (yi Fig. 4—Relationship between serum protein and ovary protein in stage IV.Amita &Saxena 1241 i =A) i t stage wae also no, tein (Figo. 1-4) in spen ’ - highest level of serum prot 7 uration int This evagests thal the serum proteins are related with ee ae ae a ane The fall of serum protein level during the spawning per! ary ed ing ovary. to reduced feeding Intensity and its utilization in he developing Present observations are in line with eee a ae ae i e life . that protein requirements change wi changes in : . 7 ‘ aise decline in protein concentration from B-9% to ieee na spawning sea water salmon. Albumin and globulin are also reported to deplete in 9. gorbuscha during spawning’. As in prosent case Dabrowski} also reported that higher plaoma proteins occur in prespawniny period, ACKNOWLEDGEMENTS Senior author Amita is thankful to C.S.1,.R. (New Delhi) for financial assistance. REFERENCES: = 4, Dabrowskl, K.R, (1982). Hydmbialogia, 94 + 3, 2. Fletcher,’C. L, Watts, £46, and King, M.J. (1975). 7. Jish Res, Bd, Can, 32 + 78, 3, Chaturvedi, L. D.. Joshi, BD. and Gupta, D.K, (1976). Matsa, 2: 16, 4. Litvinova, N.N. (1986). Bio. Nauk, 11 + as, : 5, Oser, BL. (1971). In: Hawk's Physiological Chemistry, (14th Ed.) Tata Me Graw Hill Publishing Company Ltd., New Delhi, 8. Love, RM. (1970), In : Chemical biology of fish, Academic Press, N.Y. 7. ~ “Hoar, W.S. and Randall, D.L. (1979). In : Physiology af. fishes, Margaret E, Brown - (Ed.) Academic Press Inc., New York, 8. Hatton, KE. (1988), Zirh Zu, 66 : 199, 2 Fida, S.M, Yaseen Sadri and M. Qiddiqui (1986), Kashmir Fresh Hater Diol, 20 : 64, 2