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Electrophoretic Separation

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Cont’d

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Electrophoresis

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Electrophoresis

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Electrophoresis

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Gel Electrophoresis

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Gel Electrophoresis

Different components of vertical gel electrophoresis apparatus

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Different steps in performance of vertical gel electrophoresis to resolve sample.

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Demonstration for vertical electrophoresis

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Gel electrophoresis

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Gel Electrophoresis

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Capillary electrophoresis
Basic instrumentation for capillary electrophoresis. The
sample and the source reservoir are switched when making
injections.

Cross section of a capillary

column for capillary
electrophoresis.
The dimensions shown
here are typical and are
scaled proportionally in
this figure.

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Capillaries

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Detectors

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Power supplier

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Data handling

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Capillary electrophoresis

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Sample preparation

Electrophoretic migration

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Electro-Osmotic Flow

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Electroosmotic Mobility
Schematic diagram
showing the origin of
the double layer within
a capillary tube.
Although the net
charge within the
capillary is zero, the
distribution of charge
is not. The walls of the
capillary have an
excess of negative
charge, which
decreases across the
fixed layer and the
diffuse layer, reaching
a value of zero in bulk
solution.

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Types of flow in a Capillary

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Migration in CE

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Electropherogram

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On Progress

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