Aim: Detection of Carbohydrates in the given sample

Experiment Observation Inference

Test for Carbohydrates Violet ring is formed at the Carbohydrate is present
1. Moliscn’s Test: To junction of two liquids
aqueous solution of given
food sample and 2 – 3
drops of molisch’s reagent.
Add few drops of con.
H2SO4 along the sides of
the test tube.
Test for reducing Sugars Orange red ppt of Cu2O formed Carbohydrate is reducing sugar.
2. Fehling’s Test: Fehling’s
solution A and B add to
food extract and heat in
water bath
3. Tollen’s Test : Tollen’s Silver mirror formed Carbohydrate is reducing sugar.
reagent add to food extract
and heat in water bath
Test for monosaccharide Brick red ppt formed. Carbohydrate is monosaccharide
and disaccharide
4. Barfoed’s Test:
Barfoed’s reagent is add to
food extract and heat in
water bath.
Test for Starch Blue black colour is not observed Starch is absent
5. Iodine Test: Iodine
solution add to food extract
and shake it.
Test for fats and oils
6. Solubility Test :
i) Food extract + water + Sample is miscible Fat or oil is absent
heat Sample is immiscible Fat or oil is absent
ii) Food extract alcohol +
heat
7. Acrolein Test: Food Suffocating odouris not observed Fat or oil is absent
extract + crystals of KHSO4
and heat
Test for proteins Blue colour not appeared Protein is absent.
8. Ninhydrin Test: To 1ml
of food extract and 3 – 4
drops of ninhydrin solution
and heat.
9. Biuret Test: 1ml of food Violet colour not appeared Protein is absent
extract add 1 ml of KOH,
1ml of CuSO4 solution and
heat.
10. Millon’s Test: To 1 mL No white ppt appears Protein is absent
of food extract, add 2 drops
of Millon’s regent.
Result: The given food extract contains carbohydrate it is monosaccharide and reducing sugar.
Aim: Detection of Protein in the given sample

Experiment Observation Inference

Test for Carbohydrates Violet ring is not formed Carbohydrate is Absent
1. Moliscn’s Test: To
aqueous solution of given
food sample and 2 – 3
drops of molisch’s
reagent. Add few drops of
con. H2SO4 along the sides
of the test tube.
2. Barfoed’s Test: Brick red ppt not formed. Carbohydrate is Absent
Barfoed’s reagent is add to
food extract and heat in
water bath.
Test for fats and oils
3. Solubility Test :
i) Food extract + water + Sample is miscible Fat or oil is absent
heat Sample is immiscible Fat or oil is absent
ii) Food extract alcohol +
heat
4. Acrolein Test: Food Suffocating odouris not observed Fat or oil is absent
extract + crystals of
KHSO4 and heat
Test for proteins Blue colour appeared Protein is Present.
5. Ninhydrin Test: To
1ml of food extract and 3
– 4 drops of ninhydrin
solution and heat.
6. Biuret Test: 1ml of Violet colour appeared Protein is Present
food extract add 1 ml of
KOH, 1ml of CuSO4
solution and heat.
7. Millon’s Test: To 1 mL White ppt appears which Protein contain phenolic amino acid.
of food extract, add 2 changes to brick red on boiling
drops of Millon’s regent.
Result: The given sample contains Protein.
Aim: Detection of Fat and oil in the given Sample

Experiment Observation Inference

Test for Carbohydrates Violet ring is not formed Carbohydrate is Absent
1. Moliscn’s Test: To
aqueous solution of given
food sample and 2 – 3
drops of molisch’s
reagent. Add few drops of
con. H2SO4 along the sides
of the test tube.
2. Barfoed’s Test: Brick red ppt not formed. Carbohydrate is Absent
Barfoed’s reagent is add to
food extract and heat in
water bath.
Test for fats and oils
3. Solubility Test :
i) Food extract + water + Sample is miscible Fat or oil is absent
heat Sample is immiscible Fat or oil is absent
ii) Food extract alcohol +
heat
4. Acrolein Test: Food Suffocating odouris not observed Fat or oil is absent
extract + crystals of
KHSO4 and heat
Test for proteins Blue colour not appeared Protein is Absent.
5. Ninhydrin Test: To
1ml of food extract and 3
– 4 drops of ninhydrin
solution and heat.
6. Biuret Test: 1ml of Violet colour not appeared Protein is Absent
food extract add 1 ml of
KOH, 1ml of CuSO4
solution and heat.
7. Millon’s Test: To 1 mL White ppt not formed. Protein is Absent
of food extract, add 2
drops of Millon’s regent.
Result: The given sample contains Fat and oil.