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  • Kato-Katz and Cellophane Thick Smear Techniques for Parasite Detection

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    Kim G. Sarong
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    The Kato thick Smear technique uses a cellophane-covered thick smear to detect helminth eggs with thick shells like Ascaris and Trichuris from stool samples. A small amount of stool is placed on a slide and covered with cellophane before examining under a microscope. The Kato-Katz technique also uses a cellophane-covered thick smear to detect helminth eggs and assess infection intensity, and is useful for schistosomiasis and soil-transmitted helminths. It requires a fresh stool sample, which is placed on a template and covered with cellophane before examination. Both techniques involve examining a cellophane-covered

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    The Kato thick Smear technique uses a cellophane-covered thick smear to detect helminth eggs with thick shells like Ascaris and Trichuris from stool samples. A small amount of stool is placed on a slide and covered with cellophane before examining under a microscope. The Kato-Katz technique also uses a cellophane-covered thick smear to detect helminth eggs and assess infection intensity, and is useful for schistosomiasis and soil-transmitted helminths. It requires a fresh stool sample, which is placed on a template and covered with cellophane before examination. Both techniques involve examining a cellophane-covered

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    © All Rights Reserved
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    270 vizualizări1 pagină

    Kato-Katz and Cellophane Thick Smear Techniques for Parasite Detection

    Încărcat de

    Kim G. Sarong
    The Kato thick Smear technique uses a cellophane-covered thick smear to detect helminth eggs with thick shells like Ascaris and Trichuris from stool samples. A small amount of stool is placed on a slide and covered with cellophane before examining under a microscope. The Kato-Katz technique also uses a cellophane-covered thick smear to detect helminth eggs and assess infection intensity, and is useful for schistosomiasis and soil-transmitted helminths. It requires a fresh stool sample, which is placed on a template and covered with cellophane before examination. Both techniques involve examining a cellophane-covered

    Drepturi de autor:

    © All Rights Reserved
    Descărcați ca DOCX, PDF, TXT sau citiți online pe Scribd
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    din 1

    Kato thick Smear (Cellophane thick smear)

    The cellophane-covered thick smear was originally detected for use in field surveys for helminth infections. The
    technique is useful for helminth eggs with thock shells such as Ascaris and Trichuris but not for helminth larvae or
    protozoa.
    Procedure:
    1. Label slide properly and place approximately 50-60 mg of stool (the size of a soy bean) at the center of a glass slide
    and cover with a square piece of pretreated cellophane.
    2. With the aid of a rubber stopper, press the cellophane gently to spread the stool specimen evenly to about the
    circumference of the stopper. Make sure that the specimen will not spread beyond the cellophane cover.
    3. Leave the prepared slide at room temperature for 10-20 minutes. During this time, the microscopic field becomes
    clear due to the action of glycerine on stool sample.
    4. Examine the preparation under the microscope after standing and look for helminth parasites under LPO to HPO.
    Don't allow the slides to stand for long period (more than an hour) as it causes drying and shells of hookworm will
    dissolve in glycerine solution.
    5. Report results as:
    Number of eggs of parasite name per smear
    No ova seen - if negative for parasites

    Kato-Katz Technique
    This technique is useful for assessing the intensity of infection in schistosomiasis and common soil-transmitted
    helminthiasis like ascariasis, trichuriasis and hookworm infection. Consistency of the stool is the main determinnat of
    the sensitivity of this technique. This technique can only be done on fresh stools and not in liquid-preserved samples.

    Procedure:
    1. Label slide and position the template at the center of the slide.
    2. Place the fecal sample on the cover of the stool container. Press or strain the fecal sample with the wire net. Scrape
    the feces which passed through the net then transfer them to the central hole of the card or plastic template lying over
    a clean glass slide.
    3. Fill up the hole, flatten the top level and lift the card or the template leaving the fixed amount of feces on the slide.
    Put the pre-treated cellophane over the fecal sample.
    4. With the aid of a rubber stopper, press the cellophane gently to spread the stool specimen evenly to about the
    circumference of the stopper. Make sure that the specimen will not spread beyond the cellophane cover.
    5. Leave the prepared slide at room temperature for 10-20 minutes. During this time, the microscopic field becomes
    clear due to the action of glycerine on stool sample.
    6. Examine the preparation under the microscope after standing and look for helminth parasites under LPO to HPO.
    Don't allow the slides to stand for long period (more than an hour) as it causes drying and shells of hookworm will
    dissolve in glycerine solution.
    7. Report the presence of parasites using this formula:
    # eggs of parasite/gm feces = # of eggs of parasite counter X 1000 mg
    43 mg gm
    Note: Kato-katz template is able to measure about 43 mg of stool sample; thus parasite count from this preparation
    represents parasite load in 43 mg feces.

    Parasites Light Intensity Moderate Intensity Heavy Intensity


    Ascaris lumbricoides 1-4999 epg 5000-49999 epg 50000 epg
    Trichuris trichiura 1-999 epg 1000-9999 epg 10000 epg
    Hookworm 1-1999 epg 2000-3999 epg 4000 epg
    Schistosoma 1-99 epg 100-399 epg 400 epg

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