Name: ________________________________ Period: _________ Date: ____________

2.7 Dihybrid Punnett Squares 

Activity #1: Do NOW K-L Chart
K= Know​: L= Learned:
1. A tall (Aa) plant is crossed with another tall 2 things I learned from the ​CRISPR Video ​ are…
plant (Aa). (What can CRISPR do? Why is this important?)
What percentage of the offspring will be short?
Show all of your work using a punnett square

1. 1.___________________________________________
2. What
percentage ____________________________________________
of the 2.___________________________________________
offspring will
be short?
____________________________________________

________

Activity #2: Read It​! “​Dihybrid Punnett Squares”

​ ​What if this apple also came in two sizes: small or large? CATCH Annotate CFS
What would you get if you crossed a small red apple and a large ❏ Highlight one keypoint from
green apple? Possible a medium sized brown apple. Or you may each paragraph.
still get mostly small red or large green apples, but possible some
❏ Circle 1-2 unknown/vocab
words from each paragraph
small green and large red apples. A ​dihybrid cross​ involves two ❏ Ask 1 question OR make 1
traits, such as ​color​ and size. connection
❏ Answer ​Thinking Questions
Punnett Squares and Dihybrid Crosses
1. ​What does a ​dihybrid cross
The Punnett square also allows the determination of
involve?​_______________________
genotypes and phenotypes from ​dihybrid crosses​. However,
_______________________________
a ​dihybrid cross​ only works if the genes are independent of
each other. In other words, the inheritance of an allele of one _______________________________

gene does not effect the inheritance of an allele from another 2. ​What are​ linked genes​?
gene. Genes that are close together on the same _______________________________
chromosome may not segregate independently of each other during meiosis, and are _______________________________
known as ​linked genes. _______________________________

Dihybrid crosses are more complicated than monohybrid crosses because more _______________________________
combinations of ​alleles​ are possible. 3. What is the​ Law of
independent Assortment?
According to ​Mendel​'s Second Law, ​the Law of Independent Assortment​, the inheritance _______________________________
of one trait will not affect the inheritance of another, meaning that alleles of each gene _______________________________
separate independently during gamete formation. If the genes are linked, then alleles will _______________________________
sort as a pair and not individually.
 Name: ________________________________ Period: _________ Date: ____________

Activity #3: ​ ​Level 1 Practice

In rabbits, ​grey hair is dominant​ and ​white hair is recessive​. Also in rabbits,​ ​black
eyes are dominant ​to ​red eyes are recessive​.
GG = gray hair BB = black eyes
Gg = gray hair Bb = black eyes
gg = white hair bb = red eyes

1. What are the ​phenotypes ​of rabbits that have the following ​genotypes​:
Ggbb ____________________ ggBB ________________________
ggbb ____________________ GgBb _________________________

2. A male rabbit with the ​genotype GGbb​ is crossed with a ​female rabbit with the genotype ggBb​.The
square is set up below. Fill it out and determine the phenotypes and proportions in the offspring.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

``

Questions Possible genotypes needed Answer

a. How many have ​gray hair and black eyes​? ____ out of ____ = ______%

b. How many have ​gray hair and red eyes​? ____ out of ____ = ______%

c. ​How many have ​white hair and black eyes​? ____ out of ____ = ______%

d. ​How many have ​white hair and red eyes​? ____ out of ____ = ______%

1
 Name: ________________________________ Period: _________ Date: ____________

3. A male rabbit with the genotype ​GgBb​ is crossed with a female rabbit with the
genotype ​GgBb​ The square is set up below. Fill it out and determine the phenotypes
and proportions in the offspring.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Questions Possible genotypes needed Answer

a. How many have ​gray hair and black eyes​?

____ out of ____ = ______%

b. How many have ​gray hair and red eyes​?

____ out of ____ = ______%

c. ​How many have ​white hair and black eyes​?

____ out of ____ = ______%

d. ​How many have ​white hair and red eyes​?

____ out of ____ = ______%

2
 Name: ________________________________ Period: _________ Date: ____________

4. Show the cross between a ​ggBb and a GGBb.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Phenotype Ratios:

Questions Possible genotypes needed Answer

a. How many have ​gray hair and black eyes​?

____ out of ____ = ______%

b. How many have ​gray hair and red eyes​?

____ out of ____ = ______%

c. ​How many have ​white hair and black eyes​?

____ out of ____ = ______%

d. ​How many have ​white hair and red eyes​?

____ out of ____ = ______%

3
Name: ________________________________ Period: _________ Date: ____________

Activity #4: ​ Level 2 Practice

In rabbits, ​black hair is dominant to brown hair​. Also in rabbits, ​long
straight ears are dominant to floppy ears​.
These letters represent the genotypes and phenotypes of the rabbits:

BB = black hair EE = long ears

Bb = black hair Ee = long ears
bb = brown ee = floppy ears

1. A male rabbit with the genotype​ BBee i​ s crossed with a female rabbit with
the genotype ​bbEe​ The punnett square is set up below. Fill it out and
determine the phenotypes and proportions in the offspring.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

A. How many have ​Black hair and long ears​?

● possible genotypes: _________________
● ____ out of ____ = ______%
B. How many have ​Black hair and floppy ears​?
● possible genotypes: _________________
● ____ out of ____ = ______%
C. How many have​ Brown hair and long ears​?
● possible genotypes: _________________
● ____ out of ____ = ______%
D.. How many have ​brown hair and floppy ears​?
● possible genotypes: _________________
● ____ out of ____ = ______%

2. Show the cross: ​EeBb x eeBb

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___
A. How many have ​Black hair and long ears​?
● possible genotypes: ___________________
● ____ out of ____ = ______%
B. How many have ​Black hair and floppy ears​?
● possible genotypes: _________________
● ____ out of ____ = ______%
C. How many have​ Brown hair and long ears​?
● possible genotypes: _________________
● ____ out of ____ = ______%
D.. How many have ​brown hair and floppy ears​?
● possible genotypes: _________________
● ____ out of ____ = ______%

4
 Name: ________________________________ Period: _________ Date: ____________

3. Use the following to answer the next problem:

GG = normal wings EE = red eyes
Gg = normal wings Ee = red eyes
gg = vestigial wings ee = black eyes

A fly that is​ heterozygous for both traits (GgEe) is crossed with one that has vestigial wings
and black eyes (ggee)​. Set up a Punnett square and list the phenotypic ratio of the offspring.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Questions Possible genotypes needed Answer

a. How many have ​normal wings and red eyes​?

____ out of ____ = ______%

b. How many have ​normal wings and black eyes​?

____ out of ____ = ______%

c.How many have ​vestigial wings and red eyes​?

____ out of ____ = ______%

d. ​How many have ​vestigial wings and black eyes​?
____ out of ____ = ______%

5
 Name: ________________________________ Period: _________ Date: ____________

4. Remember that:
GG = normal wings EE = red eyes
Gg = normal wings Ee = red eyes
gg = vestigial wings ee = black eyes

Show a dihybrid cross for fruit flies. ​GgEe x GgEe​. Be careful when counting the
number for each phenotype, it is easy to make a mistake. Write the phenotype
ratios below.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Questions Possible genotypes needed Answer

a. How many have ​normal wings and red eyes​?

____ out of ____ = ______%

b. How many have ​normal wings and black eyes​?

____ out of ____ = ______%

c.How many have ​vestigial wings and red eyes​?

____ out of ____ = ______%

d. ​How many have ​vestigial wings and black eyes​?
____ out of ____ = ______%

 
 
6
 Name: ________________________________ Period: _________ Date: ____________

Activity #5:​ ​Level 3 Practice

5. In horses, black coat is dominant over chestnut. Also, trotting gait is dominant over pacing gait.
BB = black coat TT = trotting gait
Bb = black coat Tt = trotting gait
bb = chestnut coat tt = pacing gait

A horse who is homozygous for black coat color and trotting gait is mated with a horse with a
chestnut coat color and pacing gait.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Questions Possible genotypes needed Answer

a. How many have ​black coat and trotting gait​?

____ out of ____ = ______%

b. How many have ​black coat and pacing gait​?

____ out of ____ = ______%

c.How many have ​chestnut coat and trotting gait​?

____ out of ____ = ______%

d. ​How many have ​chestnut coat and pacing gait​?
____ out of ____ = ______%

7
 Name: ________________________________ Period: _________ Date: ____________

6. A homozygous black horse with a pacing gait is mated with a chestnut horse with a pacing gait. For
possible offspring:

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Questions Possible genotypes needed Answer

a. How many have ​black coat and trotting gait​?

____ out of ____ = ______%

b. How many have ​black coat and pacing gait​?

____ out of ____ = ______%

c.How many have ​chestnut coat and trotting gait​?

____ out of ____ = ______%

d. ​How many have ​chestnut coat and pacing gait​?
____ out of ____ = ______%

8
 Name: ________________________________ Period: _________ Date: ____________

7. ​In humans, right-handedness is dominant over left-handedness and normal skin pigment is
dominant over albinism. If a left-handed woman who is heterozygous for normal skin pigmentation
has a child with a man who is homozygous right-handed and albino, what is the probability that the
child will be a right-handed albino?

RR = right handed SS = normal skin pigmentation

Rr = right handed Ss = normal skin pigmentation
rr = left handed ss = albinism

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Questions Possible genotypes needed Answer

a. How many have ​right handed with normal skin
pigmentation​?

____ out of ____ = ______%

b. How many have ​right handed with albinism​?

____ out of ____ = ______%

c.How many have ​left handed with normal skin
pigmentation​?
____ out of ____ = ______%
d. ​How many have ​left handed with albinism?
____ out of ____ = ______%

9
 Name: ________________________________ Period: _________ Date: ____________

8.​ Sheila is right-handed and albino (and her mother is left-handed). Doug is left-handed and has
normal skin pigmentation, but both of his maternal grandparents are albino. What is the probability of
Shelia and Doug having a right-handed child with normal skin pigmentation?

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Genotype and Phenotype probabilities:

a. How many have ​right handed with normal skin

pigmentation​?

____ out of ____ = ______%

b. How many have ​right handed with albinism​?

____ out of ____ = ______%

c.How many have ​left handed with normal skin
pigmentation​?
____ out of ____ = ______%
d. ​How many have ​left handed with albinism?
____ out of ____ = ______%

10
Name: ________________________________ Period: _________ Date: ____________

Activity #6:​ OMG ​Level Practice

1. You have decided to break into the Guinea Pig breeding business, and want to corner the
market on straight haired (g) blue(w) Guinea Pigs. Both traits require a homozygous recessive
(ggww) and are true-breeding. You have ​two​ completely heterozygous guinea pigs (GgWw).
Use the punnett square to find out the likelihood (probability) of getting a completely recessive
guinea pig.

Gametes 1: ___ ___ ___ ___ Gametes 2: ___ ___ ___ ___

Genotype and Phenotype probabilities:

●
●
●
●

11
Name: ________________________________ Period: _________ Date: ____________

2.7 Independent Tasks 

Part 1: Dihybrid Video
Watch the ​Dihybrid and Two-Trait Crosses video​ and answer the following questions:
1. What does the word “​mono” in monohybrid​ mean?

2. What is a ​dihybrid cross​?

3. What does ​Moo​ love?

4. Is the love for sinks genetic?

5. What is the phenotype of a ​HhSs​ cat?

6. What is the phenotype of a ​hhss ​cat?

7. What are ​gametes​? What do they contain?

8. What is Mendel’s Law of Segregation?

9. What is Mendel’s Law of independent Assortment?

10. Color code and label the FOIL method:

12
Name: ________________________________ Period: _________ Date: ____________

11. Each gamete must have one _________ of each gene.

12. Fill in the following dihybrid punnett square:

13. What is the ​genotype ratio​ for this cat example?

14. What is the ​phenotype ​of this cat example?

15. Are the phenotype and genotype ratios always the same?

13
Name: ________________________________ Period: _________ Date: ____________

Part 2: Probabilities video

Watch ​Probabilities, Dihybrid and Test Crosses Video​ and answer the following questions:

1. Straight wings are dominant over curly wings in fruit flies. How would you determine whether a
straight-winged fly is heterozygous or homozygous?

2. Is it possible to cross more than one trait at a time? What is this called?

3. What gamete combinations are possible when ​AABB​ alleles separate in meiosis?

4. What gamete combinations are possible when ​AABb​ alleles separate in meiosis?

5. What gamete combinations are possible when ​AaBb​ alleles separate in meiosis?

6. What gamete combinations are possible when ​Aabb​ alleles separate in meiosis?

7. How many ​genotypes​ possible in a ​BbHh x BbHh cross​?

8. How many ​phenotypes​ possible in a ​BbHh x BbHh cross?

9. Black (B) hair is dominant over brown (b) hair in rabbits. Short hair (H) is dominant over long
hair (h). If a ​homozygous black short-haired rabbit is crossed with a homozygous brown
long-haired rabbit,​ what would be the genotype and phenotype of the F1 generation?

10. List the genotypic and phenotypic ratios for the F2 offspring produced between crosses of two
animals of the F1 generation. Explain the 9:3:3:1 ratio.

14
Name: ________________________________ Period: _________ Date: ____________

11. Describe the steps taken to solve the following problem: A yellow guinea pig is crossed with a
white guinea pig. All of the offspring are cream colored. The cream-colored animals are
crossed. 16 yellow 33 cream and 15 white animals are born. Explain these results. Explain
how a white breed could be developed by starting with the two cream colored parents.

12. What is ​epistasis​?

13. What is a​ polygenic trait​? Give four examples.

14. If peas that are heterozygous for both tall stems and green seed pods are crossed, the
probability is that 75% of the offspring will be tall. The probability is also 75% of the offspring
will have green seed pods. What is the percentage of the offspring that are likely to be tall and
have green seed pods? Describe the steps to solve this problem.

15. The probability that both parents will be carriers of Cystic Fibrosis, when the incidence of
carriers among the general population is 1/20. The risk of a couple having a child with Cystic
Fibrosis when , in order to have the disease, the child must be homozygous recessive for the
trait. Describe the steps to solve this problem.

15