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Building Stronger R
& D Relations in
Bio & Pharma Sciences
through complete Technology Solutions
Complete and Customized
Biological Services and Solutions
As a unique discovery and contract research organization, iLife Discoveries provides
molecular biology and preclinical drug discovery services to pharmaceutical, biotechnology,
academia and Public & Private Institutions on an outsourced basis. With the advent of
recent scientific and technological advances, iLife Discoveries has broadened its services
& solutions portfolio in order to accelerate researchers in all phases of the early-stage
discovery process and support them in streamlining their custom discovery and
development programs. iLife also specializes in complex experimental designs, protocol
development and execution. iLife has also established itself as a leader in expertise
development through high end technology trainings in pharma and life sciences sector.
Global Presence
iLife is Headquartered in the Silicon Valley, USA, with its Global Discovery Center
establised in the Delhi NCR region of India. The global visibility and India advantage thus
converge to create attractive features of cost benefit, technology expertise, adherence to
national and international Intellectual Property rules and rapid access to qualified human
resources. With a wealth of in-house scientific expertise, world class R&D facility, and
the most advanced technologies, iLife is strategically possitioned to deliver Quality, Speed,
Competitive Costs, Flexibility, Custom Solutions and is the Contract Research Organization
of choice for the Life Sciences & Pharma Industry.
The Team
The iLife team is committed to excellence. The highly skilled scientific staff is comprised
of individuals having a breadth of expertise including preclinical, academic research, and
CRO research experience. Our research group and technical specialists have a combined
40+ years of global experience working in multiple areas of research in infectious diseases,
oncology, molecular diagnostics, bioagriculture, metabolic disorders to name a few. The
management team and staff offer their expertise in working with you to assure the success
of the design and implementation of your project, within the timelines. iLife’s commitment
to quality is second to none, piloted by an experienced leader in the life sciences domain.
Our Capabilities
Life sciences research and discovery process is highly complex, lengthy, and expensive,
yet essential for delivering safe and effective systems for improvising human healthcare.
Even though the process is reasonably well-defined, each program is fundamentally
different and shaped by many unique factors. To help our customers navigate this process,
iLife offers a comprehensive range of contract research solutions and services that span
the entire discovery continuum and can be tailored to specific research conditions. Our
portfolio covers a broad array of basic research in the molecular biology workflow and
preclinical research. We undertake research projects in cell biology, genomics, proteomics,
bioinformatics, molecular diagnostics, High throughput screening and drug discovery
studies which are designed to accelerate the pace and increase the productivity of your
discovery and development programs.
Our offerings are continually expanding based on the changing needs of our customers
and our team of experts understands and ultimately assists our customers by providing
services to span all stages of research discovery while complying with international
scientific regulations.
Consulting – Developing Solutions Together
A long-standing experience in molecular and cell biology forms the basis of iLife’s exceptional
consulting services. This service addresses the needs of research and business partners
operating in the fields of biotech, pharma and diagnostics. During the development and
standardization of a new application or protocols or instrument platform, our customers have
to make important decisions. With our flexible consulting service, we effectively support our
partners in achieving their goals. Our consultations are project specific process with a set of
recommendations for our customers. These help to define practical work flows which then
can be outsourced to iLife Discoveries. Let us sit down, join forces and develop solutions
together.
The Facility
Doing what’s best for our clients means furnishing a full range of equipment backed by a
smart, experienced team to get them through the difficult and challenging research and
discovery process. The Global Discovery Center is an ultra modern, 30,000 sq. ft., state-of-
the-art facility and houses India’s largest CLASS-10000 clean room laboratory cluster
exclusively dedicated to biological research and development for our customers. A US FDA
209E Code compliant facility is equipped with latest high throughput part 21 CFR compliant
technologies including Agilent Microarrays, Automated Liquid Handling workstations,
sequencers, HPLC, microfluidics based technologies for analysis, QPCR, OCTET RED for
biomolecular interactions, Flow Cytometers, and others.
Collaboration
Having several active and ongoing collaborative research programs, iLife continues to seek
academic, government and commercial research partnerships in HTS, diagnostics, food testing,
forensics, agriculture and drug discovery. We are interested in collaborating to pursue
government funding for joint efforts including grant applications and competitive contracts.
For gaining its own intellectual property, iLife funds several in-house drug discovery program
for the purpose of developing commercially relevant intellectual property. Contact our team
for more information on establishing a collaboration.
Anand Gupta
Founder and CEO
Dr.
Anil Mavila
President and Technology Head
Your support needs are diverse as your research and our services go far beyond traditional
notions of customer support. We will be glad to assist you with particular issues, of course –
quickly and efficiently in every step of the way. From our friendly telephone representatives to
our unmatched team of experienced technical experts and Business Coordinators, we promise
to deliver an excellent level of support you deserve from a world class organization.
If you need help choosing the right services, our experts are available to discuss your needs
and provide recommendations. Email us your contact information and area of research and
one of our experts will contact you.
Animal Research
• Identification of economic trait loci (ETL) such as
growth, meat yield etc.
• QTL mapping and marker assisted selection of the
population
• cDNA microarrays to characterize gene expression
patterns of experimental animals
• Real time PCR based assays for animal genotyping,
gene expression profiling, copy number variation
Service Information
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
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Innovating Life Through Science
Agrobiotechnology
• Identification of desirable traits in plants
Areas of Expertise
• Identification of QTL of economic traits in plants
• Genetic and physical mapping of QTL
• Construction of large insert genomic libraries
• SureSelect target enrichment for Next Gen Sequencing
• Genome sequencing strategies using Next Gen Sequencing
• Transcriptome analysis (ESTs, Microarrays, Next Gen sequencing)
• Gene expression in response to environmental stimuli (Stress – biotic & abiotic), treatment, development stage
• Gene expression profiling by microarray
• Gene expression by Real time PCR
• Epigenomics - DNA and protein modifications
• Methylation microarray
• CGH microarray
• ChIP-on-chip array
• DNA sequence analysis and data handling
• Finding genes related to stress conditions
• Finding regulatory features in gene structure by comparative genome analysis
Service Information
• Regulation of gene expression
• miRNA profiling by microarray
• ChIP-on-chip array
• Plant Pathogen (Fungal) genomics and proteomics
• Marker Screening and Discovery
• Integrative description of plant function (Productivity, Quality, Sustainability)
• GMO Testing by molecular methods
• Metabolite Analysis by HPLC
• Protein/peptide identification by off gel fractionation followed by MALDI - TOF
• Seed quality and genotype testing by molecular methods
• Testing the authenticity of Basmati rice samples by molecular methods
• Rapid wheat varietal identification
• Comprehensive identification of proteins, their isoforms, and their prevalence in each tissue
• Study of Biomolecular interaction of plant proteins (enzymes, hormones, transcription factor etc.) using OCTET technology
• Real time PCR based assays for plant genotyping, gene expression profiling, copy number variation
• Microsatellite genotyping of plant population
• Allele Mining
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Innovating Life Through Science
Microbiology and Pathobiology
Areas of Expertise
Service Information
Molecular Diagnostics
• Real Time PCR based detection of HIV, HPV, H1N1, Malaria, Hepatitis, TB
• Cancer diagnostic through mutation analysis
• Cancer diagnostic through gene expression profiling
• Bacterial and viral load detection by Real Time PCR
• Pathogen detection by molecular methods
• Real Time PCR/ PCR based detection of organisms for which microscopy gives false positive results e.g. T.vaginalis, N.gonorrhoeae.
• Molecular detection of intracellular pathogens such as M.genitalium.
• Identification of species such as Chlamydia sp.,Neisseria sp. where conventional method is less sensitive
• Molecular identification of species in which Subtyping is mandatory e.g. HSV, HPV, HCV
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Innovating Life Through Science
Oncology
• Gene expression in response to disease stage, disease
Areas of Expertise
progression (Benign & Malignant)
• Comparative Gene expression profiling in cancerous
and normal tissue by Microarray & Real Time PCR
• Identification of regulation of gene expression in
different cancers
• miRNA profiling by microarray
• ChIP-on-chip array
• Biomarker Discovery, Identification , Characterization
and Validation for different cancers
• Identification of Epigenomics modification associated
with different cancers
• CGH microarray
• Methylation microarray
• ChIP-on-chip array
• Transcriptome analysis in cancer cells (Next Gen
Service Information
Sequencing)
• Proteomics analysis in cancer cells
• Protein Profiling by 2D Gel Electrophoresis
• High throughput Protein Profiling by Antibody
microarray
• Pathway focused Protein Profiling by Antibody
microarray
• Reverse-Phase Protein Microarray
• Protein Identification by Mass Spectrometry &
N-terminal Sequencing
• Identifying tumor associated antigens
• Profiling of disease state by antibody microarray
• Time-course studies to identify different stages of
cancers
• Identification altered genes or biochemical pathways
associated with particular cancer
• To identify new molecular classes of cancer (class
discovery)
• To predict diagnosis and classification of unknown
cancer samples (class prediction)
• Study of biomolecular interaction of cancerous protein
using Biolayer Interoferometry
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Innovating Life Through Science
Drug Discovery
• SAR studies and initial pharcodynamics analysis • GPCR and kinase profiling
Areas of Expertise
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Innovating Life Through Science
Areas of Expertise
Specific Microarray based design and development of Animal free research systems for multifaceted testing of cosmetological products
can be accomplished under this program. Briefly, we develop and utilize novel cell lines representing epithelial keratinocytes, ectocervical
vaginal cells, buccal cavity cells, melanocytes and their corresponding tumor derived cell populations to study metabolism and kinetics of
small molecules and candidates for cosmeceutical applications at their molecular level. Using a proprietary technology, stable cell lines
are developed and their wild type (control) profile is characterized using advanced genomic tools. Panels thus created will be specific for
hypersensitivity reactions, toxicity responding genes, cellular apoptosis genes, aging and senescence pathways related genes and
metabolite converting enzyme panels, cytochrome p450 family members etc. These panels are used as reference standards for customized
research programs for identifying Cosmeceutical molecules with regards to their activity and toxicity. Additionally, these customized
chips will enable the researcher to resolve the time dependent responses of Cosmeceutical and therapeutic molecules.
Service Information
It has been well established that emerging economic threats associated with the safety of food and edible materials are indeed a concern
to nations that depend largely on exports from the rest of the world. Added to the complexities is the increasing threats to global food
security by engineered or man made threats such as large scale sabotage food or edible materials.
Major food pathogens like multiple species of Clostridium, Listeria, Bacillus, Yersinia, Vibrio, Botulism, Brainerd Diarrhea, Cholera,
Shigellosis etc. Specific signature sequence will be used for making specific and customized chip for targeted food products such as
Kosher meat, marine food products etc.
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Innovating Life Through Science
Service Information
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Innovating Life Through Science
Contents
GENOMICS
DNA Extraction & Purification 20 DNA Microarrays 35
RNA Extraction & Purification 20 Transcriptome Analysis/ Gene
Plasmid DNA Purification 20 Expression Analysis 36
Agilent 2100 Bioanalyzer- Technical Note 21 microRNA profiling 37
DNA Analysis 21 Location Analysis (Discover DNA Methylation
DNA Application 21 patterns) 38
Restriction digest analysis 22 Chip-on-chip Analysis 39
PCR product analysis 22 Copy Number Analysis (Comparative
Service Information
Gene expression analysis 22 Genomic Hybridization (CGH)) 40
Food analysis 22 Copy Number Variation (CNV) 41
GMO detection 22 Custom microarray development 42
Oncology 22 Real Time PCR Assays 43
Diagnostic research 22 Absolute Quantification by qPCR 44
Forensic testing 22 Relative/Comparative Quantitation 44
RNA Analysis 22 Multiplex Real Time Assay 45
RNA Application 22 Applications 45
Analysis of total RNA 23 Validation of Microarray data 45
Analysis of mRNA 23 Molecular Diagnostics 45
Analysis of Cy5-labeled samples 23 Infectious diseases 45
Analysis of T7-RNA transcripts 23 Metabolic Disorders 46
Analysis of Small RNA 23 Child and Women health 46
Restriction Fragment length Polymorphism Primer Efficiency Determination 46
(RFLP) Analysis 24 DNA/RNA copy number assessment 46
DNA Fingerprinting 25 Environmental testing using labeled probe 47
Customized PCR Assays 26 Microbial Diagnostics 47
Primer Design, Synthesis & Validation 26 Food and Agricultural 47
PCR Analysis 26 Screening of GMOs 47
Cloning 27 SNP Analysis Using Real Time PCR 47
Cloning of DNA for sequencing 27 Splice Variant Detection Using Real time PCR 47
Cloning of DNA for expression of protein 28 Cancer Diagnostics using Real Time PCR 48
Cloning and Sequencing of Up-stream/ Real Time PCR in Forensic Science 48
Down-stream Regions from Known Sequence 28 Next Gen Sequencing 49
Cloning of STRs/ Microsatellite Markers 29 Whole Genome Sequencing 49
Full-length cDNA Cloning Services 29 Whole Transcriptome Analysis 49
Library Construction 30 ChIP sequencing 50
Genomic library construction 30 Micro RNA Analysis 50
cDNA library construction 31 SureSelect Target Enrichment Systems 51
Site Directed Mutagenesis 32 Gene Synthesis 52
Gene Sequencing 33 Bacterial Identification 53
Ready to Run sequencing 33
Single Pass 33
Fragment Analysis 34
Microsatellite Analysis 34
SNP Detection 34
Gene Walking 34
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Contents
PROTEOMICS & CELL BIOLOGY BIOINFORMATICS
Protein Extraction & Purification 56
Protein Analysis 56 Introduction and Applications 74
Protein Application 56 Genomics 74
Protein Expression 56 Comparative genomics 74
Protein Purification 56 Structural genomics or structural bioinformatics 74
Antibody Analysis 56 Functional genomics 74
Food Analysis 56 Proteomics 74
Protein - Others 56 Pharmacogenomics 74
Protein Expression & Purification 57 Pharmacogenetics 74
Customized protein expression for Cheminformatics 74
E. coli, yeast and mammalian cells 57 Biomedical informatics / Medical informatics 74
Epigenetic expression of proteins under inducible Computational chemistry 74
promoters 58 In silico drug designing 75
Small Scale protein purification for proof of Agroinformatics / Agricultural informatics 75
concept studies 58 Systems biology 75
Microarray Data Analysis 76
Service Information
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GENOMICS
Service Information
Genomics
Know Your Genes...
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Nucleic Acid Purification
DNA (gDNA) is routinely extracted from various sources including quality Plasmid DNA is routinely extracted for applications like Gene
tissue (fresh and preserved), blood, saliva, other body fluids, cell Therapy & multiplex PCR, real-time quantitative PCR, DNA cloning,
cultures, plants,and forensics samples. DNA southern blot hybridization, DNA transfection, DNA
sequencing, SNPs analysis etc.
Service Information
DNA Analysis 21
Plasmid Analysis 21
RNA Analysis 22
RFLP 24
AFLP 24
DNA Fingerprinting 25
Customized PCR Assays 26
Cloning 27
Library Construction 30
Site Directed Mutagenesis 32
Gene Sequencing 33
DNA Microarrays 35
Next Gen Sequencing 49
High-end Technology Training 88
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Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
Quantification of Biomolecules such as DNA, RNA and protein is an essential, integral and fundamental step for any life science research.
It is based on Lab-on-a-Chip technology which enables downscaling and integration of several experimental steps into one process, in
combination with software which automates data analysis and data archiving and generates a complete QC report with quantitation
estimate, virtual gel image and electropherogram.
GENOMICS
The Lab-on-a-Chip Approach
Service Information
The Chip technology uses microfluidics whose behavior, precise control and manipulation of fluids that are geometrically constrained to a
small, typically sub-millimeter, scale allows quantification of biomolecules with a minimum volume of 1ul. The sample moves through the
microchannels from the sample well and it is injected to the separation channel. The sample components are electrophoretically separated
and are detected by their fluorescence and translated into gel like images (bands) and electropherograms (peaks).
DNA Analysis
Agilent 2100 Bioanalyzer is used which is a microfluidics based platform for sizing, quantification
and quality control of DNA. Different kits allows to automatically size and quantitate PCR
fragments and restriction digests accurately and reproducibly
DNA Application
The chip technology can perform variety of applications in the life science research such as
sizing and quantitation of DNA fragments like PCR product purity, Multiplex PCR Applications,
GMO Testing, Pathogen Detection, Genotyping Applications (Duplication/Deletion, Allele
frequency), Cancer diagnostics etc...:
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Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
Separation of different mixtures of PCR products GDA002 Detection of single base mutations
GDA003 Separation of different mixtures of PCR products
Determination of PCR product impurity GDA004 Determination of PCR product impurity
Multiplex PCR analysis of bacteria in chicken GDA005 Multiplex PCR analysis of bacteria in chicken
GDA006 mRNA expression study by
Gene expression analysis comparative multiplex PCR
GDA007 Standardized end-point RT-PCR
mRNA expression study by comparative multiplex PCR GDA008 Co-amplification and response curves
Standardized end-point RT-PCR GDA009 Competitive PCR
Co-amplification and response curves GDA010 Development of meat specific assays
Competitive PCR GDA011 Sea food authencity testing
GDA012 Fish species identification by RFLP
GDA013 Strawberry and Raspberry food differentiation.
Food analysis GDA014 Basmati Rice authencity testing
Development of meat specific assays GDA015 Development of a multiplex assay for soya
Sea food authencity testing GDA016 DNA stability during food processing
GDA017 GMO detection by nested multiplex PCR
Fish species identification by RFLP GDA018 Tumor cell detection from carcinoma patient blood
Service Information
Strawberry and Raspberry food differentiation. GDA019 SNP analysis in cancer related P16 gene
GDA020 K-ras gene SNP detection
GMO detection GDA021 METH-2 downregulation in lung carcinomas
GDA022 Label-free analysis of microsatellite
Basmati Rice authencity testing instability in carcinoma
Development of a multiplex assay for soya GDA023 Genotyping of H. pylori
DNA stability during food processing GDA024 Duplications and deletions in genomic DNA
GMO detection by nested multiplex PCR GDA025 Optimization of PCR on mtDNA
GDA026 Customized DNA Analysis
Oncology
Tumor cell detection from carcinoma patient blood For all prices contact : customersupport@ilifediscoveries.com
SNP analysis in cancer related P16 gene
K-ras gene SNP detection RNA Analysis
METH-2 downregulation in lung carcinomas Agilent 2100 Bioanalyzer is used which is a microfluidics based
Label-free analysis of microsatellite instability in carcinoma platform for Industry standard of RNA quality control. RNA Integrity
Number (RIN), a quantitation estimate, and ribosomal ratios of the
Diagnostic research
total RNA sample will be provided. For mRNA samples the
Genotyping of H. pylori
percentage of ribosomal impurities is calculated.
Duplications and deletions in genomic DNA
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Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
GENOMICS
Genomic DNA contamination a controlled manner through the microchannels which are
hydrodynamically focused to a portion of a channel by a side stream
Analysis of mRNA of buffer and cells pass the fluorescence detector in a single file
RNA integrity and each event is monitored in a histogram or a dot plot.
Ribosomal RNA contamination in mRNA samples
It can be used for many applications such as Apoptosis,
Analysis of Cy5-labeled samples
Analysis of cRNA with and without dye in gel matrix Transfection efficiency monitoring, protein expression monitoring
Optimization of labeling reactions and gene silencing.
cRNA fragmentation
Service Information
Analysis of Small RNA
Note: We offer Customized solutions for nucleic acid applications. Figure: Flow cytometry on a chip. Two Color detection and a dot plot
Please contact technical support for further assistance. view for easy data evaluation.
Ordering Information
Service No. Description
GRA001 RNA integrity and Documentation of RIN number
GRA002 Reproducibility of quantitation
GRA003 Detection of low levels of RNA
GRA004 RNA integrity for pico gram level detection
GRA005 Genomic DNA contamination
Figure : Electropherogram of RNA analysis GRA006 RNA integrity
GRA007 Ribosomal RNA contamination in mRNA samples
GRA008 Analysis of cRNA with and without dye in gel matrix
Protein Analysis GRA009 Optimization of labeling reactions
GRA010 cRNA fragmentation
A specific chip design allows the analysis of upto 10 protein samples GRA011 Size estimation
in the 5-250KDa size range with the use of different kits. A variety GRA012 Analysis of small RNA
of different samples for can be used such as column fractions, GRA012 Customized RNA Analysis
purified proteins, antibodies and cell lysate.
For all prices contact : customersupport@ilifediscoveries.com
Figure : Direct Comparison of samples run on SDS PAGE with silver staining
and on Agilent Bioanalyzer. Concentrations are given per lane.
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Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
7500 base pairs (DNA 7500) and 100 to 12000 bp (DNA 12000)
RFLP Analysis services are offered for the following respectively. These kits can be used to analyze, for example, PCR
and RT-PCR products as well as restriction digests.
applications:
• Genome mapping
• Cloning
• Localization of genes for genetic disorders
• Determination of risk for disease
• Genetic fingerprinting
• Characterization of genetic diversity
• Breeding patterns in animal populations
And many more……
Service Information
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Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
DNA Fingerprinting
Fingerprints are able to determine whether two DNA samples are
from the same person, related people, or non-related people. Small
numbers of sequences of DNA that are known to vary among
GENOMICS
individuals are analyzed to get a certain probability of a match.
Applications:
• Paternity and maternity testing
• Criminal Identification and Forensics
• Personal Identification
Service Information
Fragment Analysis Ordering Information
Agilent 2100 Bioanalyzer is used which is a microfluidics based
Service No. Description
platform for sizing, quantification and quality control of DNA.
GDF001 DNA fingerprinting, fragment sizing by
agarose gel and fragment analysis by sequencing
Different kits allows to automatically size and quantitate PCR GDF002 DNA fingerprinting, fragment sizing with
fragments and restriction digests accurately and reproducibly. bioanalyzer and fragment analysis by sequencing
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Nucleic Acid Analysis on Agilent 2100 Bioanalyzer
done on High-end Lab-on- Chip technology. Different kits allows to automatically size and quantitate restriction
digests accurately and reproducibly:
Custom PCR Assays services are offered by iLIFE for the
following applications:
• Amplification & Quantification of DNA
• Selective DNA isolation for genetic Fingerprinting, generating
hybridization probes for Southern or northern hyb. and DNA
Cloning, DNA sequencing etc.
• Diagnosis of diseases
• Species identification
• Multiplex-PCR for analysis of microsatellites and SNPs etc.
And many more……
Oligonucleotide Design
(Primer pair designing using
software)
Ordering Information
Service No. Description
Optimization of Primers GPR001 Primer Designing, Synthesis and Validation
GCP001 CustomPCRAssay
(Thermal profile,
Non-specificity)
For all prices contact : customersupport@ilifediscoveries.com
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Cloning
Cloning of DNA for Sequencing
Sequencing of PCR products using terminal primers (used for
amplification) would usually generate incomplete sequence data,
as it does not read the terminal 50 -100 bases on both ends. iLife
GENOMICS
Discoveries offers the service for cloning and sequencing of PCR
products in our standard vector that would enable the scientists to
acquire complete and analysed sequence data of their PCR
products.
You can give us cells, genomic DNA, RNA and PCR product. This
will be followed by verification of recombinant plasmid by restriction
mapping. Derived clone can be further used for:
• Generation of mutants
• Subcloning
Service Information
Note: Beyond 1 kb sequence service, each 1 kb will be charged
extra as sample.
Deliverables:
1. Clone DNA (up to 5 µg)
2. Sequence data (bi-directional, raw data)
3. Final analysed sequence data
4. Project Report
1. The band of interest should be the most prominent product within Ordering Information
the PCR sample.
2. The samples should not have excessive smearing. Service No. Description
3. Please indicate whether the PCR product has a higher GC – GCL007 Cloning of DNA of < 1 kb with Primers provided
content
GCL008 Cloning of DNA of 1 kb with Primers provided
GCL009 Cloning of DNA of 2 kb with Primers provided
GCL010 Cloning of DNA of 3 kb with Primers provided
GCL018 Cloning DNA of < 1 kb including Primer
designing and synthesis
GCL019 Cloning DNA of 1 kb including Primer
designing and synthesis
GCL020 Cloning DNA of 2 kb including Primer
designing and synthesis
GCL021 Cloning DNA of 3 kb including Primer
designing and synthesis
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Cloning
Cloning of DNA for Expression of Protein
iLife can assist in cloning any gene in any vector for expression of Ordering Information
the protein product. Based on the requirement, the derived protein
can have: Service No. Description
GENOMICS
Note: Beyond 1 kb sequence service, each 1 kb will be charged as For all prices contact : customersupport@ilifediscoveries.com
extra sample.
Deliverables :
1. Cloned DNA containing up to 2 kb of up-stream/down-stream
sequence
2. Bi-directional sequence data (raw data as well as analyzed and
aligned data) with the putative regulatory regions indicated
3. Project report
Ordering Information
Service No. Description
GCL051 Cloning and Sequencing of Up-stream/
Down-stream Regions from Known
Sequence with Primers provided
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Cloning
Cloning of STRs/ Microsatellite Markers This service will include:
• Cloning into any commercially available cloning and/ or
Genotyping is a technology that can distinguish one sample from expression vector
another. The technology takes advantage of the Short Tandem • Cloning and sequencing of full-length cDNA irrespective of the
Repeats (STRs) or Microsatellites that occur dispersed through out gene size and the number of introns present.
GENOMICS
the genomes. The STRs are more often than not polymorphic in • Fast and reliable service
nature and the number of repeats occurring in each STR locus
varies within samples. STRs that are linked/segregate with traits Deliverables:
are used for Market Assisted Selection (MAS) in breeding programs. 1. Full-length cDNA into a cloning vector
Cloning and characterization of STRs that might demonstrate 2. Bi-directional sequence data (both raw data and analysed data)
polymorphism within very similar samples are important for DNA 3. Project report
fingerprinting/ genotyping purposes.
Service Information
• Standardization of STR specific “PCR/multiplex PCR” conditions
as well as screening samples for polymorphism are also
undertaken (optional)
Deliverables :
1. 10 clones with STRs/ minimum
2. Sequence data and electropherogram
3. Final report
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Library construction
Genomic Library Construction
GENOMICS
Service Information
Genomic DNA libraries are commonly used when one wants to Deliverables:
preserve the entire genome of an organism. The library thus formed 1. Library delivered on plates, filters or glycerol stock
will help the scientist to study the entire genome, including large 2. Project Report
numbers of non-coding and regulatory regions from the genome.
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Library construction
cDNA Library Construction
GENOMICS
Service Information
cDNA libraries are commonly used when one wants to reproduce Deliverables:
only the coding regions of the eukaryotic genomes, and the amount 1. Library delivered on plates, filters or glycerol stock
of information is reduced to remove the large numbers of non-coding 2. Project Report
and regulatory regions from the library.
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Site Directed Mutagenesis
iLife offers a complete mutagenesis service for creating single
multiple point mutations, deletions, insertions or random mutations
into any gene of your interest.
Deliverables :
• 4 ug lyophilized destination vector containing the gene
• Sequence chromatograms covering your gene (electronic)
• Construct map for the plasmid (electronic)
• Project Report
Ordering Information
Service No. Description
GSM001 Plasmid <7kb, single point mutation
GSM002 Plasmid <7kb, multiple point mutations
GSM003 Plasmid >7kb, single point mutation
GSM004 Plasmid >7kb, multiple point mutations
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Gene Sequencing
Ready to Run Sequencing Samples - Candidate gene PCR product/ whole genomic DNA/
Cell culture plates/ Cloned PCR Product.
Ready to run sequencing service is provided for the following:
SNP discovery
Targeted re-sequencing approach for accurate polymorphism
GENOMICS
• PCR Products identification with Sanger sequencing.
• Plasmid
• BACs High quality sequence reads are provided. Long sequences are
provided with maximum accuracy using long capillaries.
Sample required is sequence ready purified amplicons & result is
provided in the form of long read length, high quality sequence
data. Ordering Information
Service No. Description
GSP001 Single Pass sequencing from Culture plates
(Using Universal Primers)
GSP002 Single Pass sequencing from gDNA (Using Universal Primers)
GSP003 Single Pass sequencing from Plasmid (Using Universal Primers)
GSP004 Single Pass sequencing from RNA (Using Universal Primers)
GSP005 Single Pass sequencing from cDNA (Using Universal Primers)
Service Information
GSP006 Single Pass sequeincing from cloned vectors
(Using Universal Primers)
GSP07 Single Pass sequencing from BAC/YAC (Using Universal Primers)
GSP08 Single Pass sequencing from non-purified PCR amplicons
(Using Universal Primers)
GSP09 Single Pass sequencing from purified PCR amplicons
(Using Universal Primers)
GSP010 Single Pass sequencing from Culture plates
(Using Custom Primers)
GSP011 Single Pass sequencing from gDNA (Using Custom Primers)
GSP012 Single Pass sequencing from Plasmid (Using Custom Primers)
GSP013 Single Pass sequencing from RNA (Using Custom Primers)
GSP014 Single Pass sequencing from cDNA (Using Custom Primers)
GSP015 Single Pass sequeincing from cloned vectors
(Using Custom Primers)
Ordering Information GSP016 Single Pass sequencing from BAC/YAC (Using Custom Primers)
GSP017 Single Pass sequencing from non-purified PCR amplicons
(Using Custom Primers)
Service No. Description GSP018 Single Pass sequencing from purified PCR amplicons
GRR001 Ready to run gDNA amplicons (Using Custom Primers)
GRR002 Ready to run Plasmid amplicons
GRR003 Ready to run BAC amplicons For all prices contact : customersupport@ilifediscoveries.com
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Gene Sequencing
Ordering Information
Fragment Analysis
Service No. Description
Fragment Analysis services for almost all types of fragments like GFA008 Microsattelite analysis using custom labelled primers
AFLP, SNP, SSR, STR, VNTR etc. are provided for the following (for multiplexing;4plex)
applications: GFA009 Microsattelite analysis using custom labelled primers
GENOMICS
For high-throughput analysis, multiplex reactions with primers are available for the following.
labeled with different dyes (5 plex maximum) & fragment sizes can
be performed. • PCR amplicons
• Plasmid inserts
• Entire plasmids
Sample submission can be:
• Genomic DNA to produce single strand
• Genomic DNA with primer details
• Culture Plates
• Pre-labeled primers for multiplexing along with the fragments
• BAC or Phage DNA
and thermocycler conditions
• Primer sequences in excel format with fragments (specifying the
Beginning from the reference sequence, stage one of sequencing
labeling type) and thermocylcler conditions
is started, and then onwards, primers are designed to perform
• PCR amplicon pool of the multiplexed reaction for sequencing
overlapping sequencing of the remaining DNA and assemble the
data generation and analysis
sequence data. Additionally, if you have a known sequence for
which you need quality resequencing information, a complete set
Highly accurate data is provided with less than 1bp difference
of overlapping primers will be designed for generating complete
between the sample replicates.
sequence.
SNP Detection
• Upto 4kb of amplicons can be generated.
Single nucleotide polymorphisms (SNPs) are widely used as DNA
• Contigs are assembled using refined bioinformatics tools and
markers in genetic studies. Compared to other SNP genotyping
high quality amplification & sequencing primers are designed
methods, sequencing is in particular, suited to identifying multiple
for Gene walks.
SNPs in a small region, such as the highly polymorphic Major
Histocompatibility Complex region of the genome.
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DNA Microarrays
Microarrays are now most extensively used and reliable tools for With wide range of applications iLife helps scientists enhance their
exploring the transcription events of any living cell. Microarrays can drug discovery research.
be a great tool while conducting basic research, understanding
disease mechanisms, exploring stress and environmental tolerance Gene Expression Analysis
mechanisms, identifying new drug targets end discovering • Explore transcriptome through expression profiling
GENOMICS
biomarkers. • Transcription regulators analysis by microRNA profiling
iLife scientists explore a wide variety of genomes across different Location analysis
model systems using the Agilent’s DNA microarray platform. • Explore epigenetic modification by methylation analysis
• Discover protein-DNA interactions through ChIP-on-chip analysis
Focused on Sensitivity
High-purity, high-fidelity 60-mer oligonucleotide probes deliver better
signal-to-noise-ratios and wider dynamic ranges than other
Copy Number Analysis
microarrays. • Explore the chromosomal variations through Comparative
Genomic Hybridization (CGH)
• Discover DNA Copy Number Variants (CNVs) across the
Engineered for Flexibility chromosomes
The SurePrint inkjet technology, enables total control over array
content with no compromise data quality.
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Optimized for Scalability
The multiplexed arrays have up to eight arrays on a single slide,
making expression studies of any scale feasible and flexible.
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DNA Microarrays
Transcriptome Analysis
Gene Expression Analysis
Global gene expression profiling has long been a staple of genomics
GENOMICS
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36 (US, Canada & Europe) Call +1 408 - 907 6645
DNA Microarrays
Ordering Information microRNA Profiling
Service No. Description Discover Novel Pathways of Gene Regulation
GGE020 Bovine Genome Microarray Two Color
GGE021 Canine Genome Microarray One Color The discovery of microRNAs (miRNAs) has revolutionized our
GENOMICS
GGE022 Canine Genome Microarray Two Color understanding of the regulatory arsenal of the cell. microRNAs are
GGE023 C. elegans Genome Microarray One Color key regulators of gene expression and have been found to be
GGE024 C. elegans Genome Microarray Two Color
modulated in different developmental stages, tissues and disease
GGE025 Corn Genome Microarray One Color
GGE026 Corn Genome Microarray Two Color states.
GGE027 Drosophila Genome Microarray One Color
GGE028 Drosophila Genome Microarray Two Color iLife offers hybridization based miRNA microarray services to profile
GGE029 E.Coli Genome Microarray One Color
entire human, mouse and custom organism miRNAs.
GGE030 E.Coli Genome Microarray Two Color
GGE031 Horse Genome Microarray One Color Hybridization-based microarrays provide maximum flexibility in
GGE032 Horse Genome Microarray Two Color terms of species of interest and target coverage.
GGE033 Magnaportha Genome Microarray One Color
GGE034 Magnaportha Genome Microarray Two Color
Entire workflow starting from isolating very difficult microRNA from
GGE035 Mosquito Genome Microarray One Color
GGE036 Mosquito Genome Microarray Two Color various sample sources till the data analysis is performed in well
GGE037 Pig Genome Microarray One Color optimized and clean room facility ensuring high quality and
GGE038 Pig Genome Microarray Two Color publishable data. Reliable biological interpretation data will be
Service Information
GGE039 Rabbit Genome Microarray One Color
provided from the data analysis report.
GGE040 Rabbit Genome Microarray Two Color
GGE041 Rat Genome Microarray One Color
GGE042 Rat Genome Microarray Two Color The flexible format (8x15K) and service can handle projects focusing
GGE043 Rhesus Monkey Genome Microarray One Color on a small set of candidate microRNAs as well as screening studies
GGE044 Rhesus Monkey Genome Microarray Two Color
designed to profile majority of currently known microRNAs.
GGE045 Rice Genome Microarray One Color
GGE046 Rice Genome Microarray Two Color
GGE047 Salmon Genome Microarray One Color
GGE048 Salmon Genome Microarray Two Color
GGE049 Sheep Genome Microarray One Color
GGE050 Sheep Genome Microarray Two Color
GGE051 Tobacco Genome Microarray One Color
GGE052 Tobacco Genome Microarray Two Color
GGE053 Xenopus Genome Microarray One Color
GGE054 Xenopus Genome Microarray Two Color
GGE055 Yeast Genome Microarray One Color
GGE056 Yeast Genome Microarray Two Color
GGE057 Yeast Genome Microarray One Color
GGE058 Yeast Genome Microarray Two Color
GGE059 Zebrafish Genome Microarray One Color
GGE060 Zebrafish Genome Microarray Two Color
Ordering Information
Service No. Description
GGE061 Human miRNA Microarray
GGE062 Mouse miRNA Microarray
GGE063 Rat miRNA Microarray
GGE064 Custom miRNA Microarray
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DNA Microarrays
Location Analysis
Discover DNA Methylation Patterns
DNA methylation events are heritable modifications that regulate
GENOMICS
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DNA Microarrays
Chip-on-chip Analysis
Transcription is a complex process that requires multiple interactions
and orchestrated binding of numerous components. Thus, traditional
gene expression analysis provide only partial glimpses into gene
GENOMICS
regulation, whereas ChIP-on-chip reveals a high-resolution map of
transcriptional activities, including activation and repression.
By partnering, we enable you to obtain insightful and robust profiles
of DNA-protein binding and promoter occupancy across entire
genomes.
Service Information
Genome-Wide Location Analysis
We help scientists explore the genome and perform location analysis
through ChIP-on-chip microarray featuring a total of ~244,000 60-
mer probes. Probes are spaced every ~100-300 bp across regions
of interest in both coding and noncoding DNA sequences.
Focused microarray designs include human and mouse promoter
sets. Whole genome designs include human, mouse, Drosophila,
C. elegans, and Arabidopsis microarray sets.
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DNA Microarrays
Copy Number Analysis
Comparative Genomic Hybridization (CGH)
Genomic instability is a classic hallmark of cancer genetic disorders.
GENOMICS
• Evolutionary Biology
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DNA Microarrays
Copy Number Variation (CNV)
Genetic variation is what makes each human being unique, and
determines our susceptibility to diseases. In recent years,
researchers have utilized SNPs for the identification of genetic
GENOMICS
markers for complex and common diseases; however, this approach
has only revealed part of the picture. Copy number variants (CNVs)
are reshaping our understanding of the genetics of a growing list of
diseases, and providing a deep insight into the role that these
variants play in genetic diseases. The use of high-resolution aCGH
microarrays to study CNVs enables researchers to get an extremely
fine view of variant structure and impact.
Service Information
CNV microarrays are available in 1x244k and 1x1M formats
depending on the overall spacing of the tiled probes representing
human coding and noncoding sequences.
Ordering Information
Service No. Description
GCN023 Human Genome 1 x 244K CNV Microarray Kit
GCN024 Human Genome 2 x 105K CNV Microarray Kit
GCN025 Human Catalog 4 x 44K CNV Microarray Kit
GCN026 Mouse Genome 1 x 244K CNV Microarray Kit
GCN027 Mouse Genome 2 x 105K CNV Microarray Kit
GCN028 Rat Genome 1 x 244K CNV Microarray Kit
GCN029 Rat Genome 2 x 105K CNV Microarray Kit
GCN030 Chicken Genome 2 x 105K CNV Microarray Kit
GCN031 Custom 1 x 244K HD-CNV Microarray,
GCN032 Custom 2 x 105K HD-CNV Microarray,
GCN033 Custom 4 x 44K HD-CNV Microarray
GCN034 Custom 8x15K HD-CNV Microarray
GCN035 SurePrint G3 custom 1x1M CNV array
GCN036 SurePrint G3 custom 2X400K CNV array 2x400K
GCN037 SurePrint G3 custom 4x180K CNV array
GCN038 SurePrint G3 custom 8x60K CNV array
GCN039 Cyto Custom 1 x 244K HD-CNV Microarray
GCN040 Cyto Custom 2 x 105K HD-CNV Microarray
GCN041 Cyto Custom 4 x 44K HD-CNV Microarray
GCN042 Cyto Custom 8x15K HD-CNV Microarray
GCN043 SurePrint G3 2x400K Human CNV array
GCN044 Human catalog 2x105k CNV Microarray kit
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DNA Microarrays
Custom Microarray Development Ordering Information
Service No. Description
Whether studying the genome of a unique organism or digging GGE007 Custom 4 x 44K Microarray GE One Color
deep into a geneset of interest, customization is possible for any GGE008 Custom 4 x 44K Microarray GE Two Color
GENOMICS
genome, any application, anytime and anywhere. GGE009 Custom 2 x 105KMicroarray GE One Color
GGE010 Custom 2 x 105K Microarray GE Two Color
GGE011 Custom 1 x 244K Microarray GE One Color
iLife helps you design microarrays and exclusively customize arrays
GGE012 Custom 1 x 244K Microarray GE Two Color
for specific applications including : GGE013 Custom 8 x 15K Microarray GE Two Color
• gene expression GGE014 Custom 8 x 15K Microarray GE Two Color
• microRNA profiling GGE064 Custom miRNA Microarray
GLA013 Custom 1x244K DNA Methylation Single Owner
• DNA methylation
GLA014 Custom 4x44K DNA Methylation Single Owner
• Comparative genomic hybridization (aCGH) GLA015 Custom 2x105K DNA Methylation Single Owner
• ChIP-on-chip GLA016 Custom 8x15K DNA Methylation Single Owner
• model organisms GLA017 SurePrint 1X1M G3 CH3 Custom
GLA018 SurePrint 2X400K G3 CH3 Custom
GLA019 SurePrint 4X180K G3 CH3 Custom
We help you select probe content from wide range of high definition GLA020 SurePrint 8X60K G3 CH3 Custom
databases present on Agilent eArray portal. Oligonucleotide probes GLA033 Custom 1x244K ChIP-on-Chip Single Owner
are also custom designed from the genes and transcript annotations GLA034 Custom 4x44K ChIP-on-Chip Single Owner
Service Information
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42 (US, Canada & Europe) Call +1 408 - 907 6645
Real Time PCR Assays
Real Time QPCR Assays
Real Time quantitative PCR is used as a quantitative tool for gene
expression nowadays. QPCR is a powerful, highly sensitive
technique that enables both detection & quantitation (as absolute
GENOMICS
number of copies or relative amount when normalized to DNA input
or additional normalizing genes) of one or more specific sequences
in a DNA sample. iLife Discoveries supports both Taqman & SYBR
Green chemistries for detection of products in real time PCR.
Service Information
Real Time QPCR delivers powerful capabilities for generating and
analyzing data for variety of applications:
Ordering Information
Service No. Description
GRT001 SYBR-Green based QPCR
GRT002 Taqman-based QPCR
GPR001 Primer Designing, Synthesis and Validation for
SYBR-Green Detection
GPR002 Probe & Primer Designing, Synthesis and
Validation for Taqman Detection
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Real Time PCR Assays
Absolute Quantification by qPCR Relative/Comparative Quantitation
Real Time qPCR can be used for Absolute Quantification of unknown Comparative quantification of genes is an application of RT-PCR
samples. It is a method used to quantify unknown samples compared which is used for calculation of relative abundance of a gene in
to samples of known concentration. The Ct values of the unknowns treated sample with respect to the control sample in terms of fold
GENOMICS
can now be compared to the standard curve to determine the initial change or copy number.
quantity of template in these samples. We offer complete solution from sample preparation to data analysis
which includes Relative Quantity Charts where relative
We offer complete solution from sample preparation to data concentration of gene of interest is measured in unknown sample
generation which includes standard curve, Initial template quantity, compared to the control.
Threshold cycle number, amplification plots, plate sample values,
text report. Data is generated in the form of amplification plots, raw fluorescence
values, plate sample values and text report.
Our services can be utilized for applications of :
• Viral Load Detection We can perform relative quantitation services for applications of :
• Genomic and Mitochondrial DNA Detection (Forensics) • Gene Expression
• DNA/RNA Copy number ssessment • Microarray data validation
• Primer Efficiency determination • Pathogen Detection
• GMO Detection
Service Information
• Environmental Testing
Sample submission Requirement : • Infectious Diseases
• Standards for plotting of standard curve
• Unknown samples for quantification Sample Submission Guidelines
• Calibrator (a positive control of known concentrations that all
samples will be compared for the up-regulation and down
regulation of gene levels)
• Infected samples containing unknown concentration of gene of
interest
• Housekeeping gene required for constant level expression in
calibrator and infected samples.
Ordering Information
Service No. Description
GAQ001 Absolute Quantitation with Standard Curve
GAQ002 Absolute Quantitation without Standard
Curve
Ordering Information
For all prices contact : customersupport@ilifediscoveries.com
Service No. Description
GRQ001 Relative SYBR Green Quantitation with House Keeping genes
GRQ002 Relative SYBR Green Quantitation without House Keeping genes
GRQ003 Relative Taqman Quantitation with House Keeping genes
GRQ004 Relative Taqman Quantitation without House Keeping genes
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Real Time PCR Assays
Multiplex Real Time Assay Applications
Multiplex QPCR allows one or more targets of interest to be Validation of Microarray data
amplified with a normalizing gene in the same reaction vessel by Quantitative real-time PCR (qPCR) is a commonly used validation
using probes with spectrally different fluorophores for the detection tool for confirming gene expression results obtained from microarray
GENOMICS
of each amplicon. Generally experimental projects require many analysis.
runs on the same set of genes. Microarray analysis performs hundreds or thousands of differentially
expressed genes simultaneously while qPCR provides precise
iLife provides cost effective and higher level of statistical correlation quantification of a wide dynamic range of expression levels due to
to design a multiplex reaction to use for the duration of the study. its increased sensitivity and reproducibility. We can rapidly validate
The multiplex reaction is important when the template material is and correlate microarray data through the qPCR approach.
limited as this approach allows maximum amount of data to be We identify real gene specific variation, as it is important to
generated from each assay. The primers and probes for multiplex normalize gene expression level by carefully selected stable internal
reactions will be designed the same way as for singleplex reactions control genes.
with a few extra considerations.
Gene Expression Workflow
We offer complete solutions from Sample preparation to data
analysis which includes raw fluorescence data, amplification Sample preparation and isolation of RNA
Service Information
plots, threshold, dissociation curve (only in case of SYBR
Green) & standard curve Amplification and labeling
Molecular Diagnostics
The real time PCR provides high-throughput method for faster and
Ordering Information more sensitive and more specific methods to diagnose viral
pathogens.
Service No. Description
GMR001 Multiplex QPCR Assay
Infectious diseases
The prevalence of disease in patients is exacerbated by inadequate
For all prices contact : customersupport@ilifediscoveries.com methods for pathogen detection. PCR-based amplification
approaches have been developed to address this problem because
conventional methods for pathogen identification lack sensitivity,
specificity and speed, and some infectious organisms are difficult
to culture. PCR amplification of ribosomal genes and their internal
transcribed spacer regions coupled with sequence-specific
detection probes are the most reliable approaches for disease
identification.
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Real Time PCR Assays
Metabolic Disorders Serial Dilution Standard Curve
The introduction of real-time PCR provides the opportunity for the
rapid detection of pathogens in food and clinical settings. Apart
from saving time, real-time PCR is highly specific and sensitive
and offers the potential for quantification .The risk of cross-
GENOMICS
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Real Time PCR Assays
Environmental Testing using Labeled Probe when coupled with the advanced technology of RT thermal cyclers
with reduced ramp times, greatly reduces cycle times so that
Micro-organism population dynamics assessed by real time amplified targets can be recognized within 30 min of amplification
quantitative PCR in the environment. A real time quantitative PCR in some cases
(QPCR) is used to detect and quantify the 16S rRNA gene
GENOMICS
concentration Screening of GMOs
A Real time PCR screening is done to detect specific strains of Q-PCR is used to measure the quantity of a PCR product. It is the
micro-organisms in environmental samples. Fluorescently labeled method of choice to quantitatively measure amounts of transgene
probe are designed against the targets i.e. a short segment of the DNA in a food or feed sample.If the targeted genetic sequence is
micro-organism DNA which is highly specific to the probe unique to a certain GMO, a positive PCR test proves that the GMO
is present in the sample.
Service Information
and genotypic data.
Real Time PCR allows a fast, sensitive and elegant quantification
of genotypes based on SNP. We provide a customized solution for
accurate quantification of SNP allele-specific respective informative
locus analysis using real time PCR. The SNP analysis can be useful
in the following areas:
• Allelic Association
Microbial Diagnostics
• Linkage Disequilibrium
Initially, implementation of the polymerase chain reaction (PCR)
• Positional Cloning
resulted in sensitive detection of microbes that were hard or
• Disease Mapping
impossible to diagnose by conventional diagnostic procedures such
• Pharmacogenetics
as culture and serology. A further improved version is now rapidly
• Mutational Analysis
replacing end-point detection PCR in many diagnostic laboratories.
In real-time PCR, amplified products are detected by fluorescence
at the moment that they are generated and directly related to the References
input target quantity, so that quantitation is possible. 1. Collins A.L., Lonjou C., Morton N.E. (1999) Proc. Nat. Acad. Sci.
96:15173–15177.
The probe includes an internal amplification control and primers 2. Lander E.S. (1996) Science 274:536–539
and a probe designed to target a highly specific part of the genes. 3. Lander E.S., Schork N.J. (1994) Science 265:2037–2048.
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Real Time PCR Assays
Cancer Diagnostics using Real Time PCR Ordering Information
Cancer-specific Molecular Markers can be used to provide accurate
Service No. Description
prognosis and to predict response, resistance, or toxicity to therapy.
GQA001 Validation of Microarray data
The diversity of genomic alterations involved in malignancy
GQA002 Molecular Diagnostics for Infectious diseases
necessitates a variety of analysis for complete tumor profiling in
GENOMICS
Real Time PCR in Forensic Science For all prices contact : customersupport@ilifediscoveries.com
Quantification of DNA in a forensic sample is of major importance
for proper DNA amplification and STR profiling. The implementation
of molecular analysis in the forensic laboratory has been a
tremendous benefit to the criminal justice community.
Investigation of forensic samples using real time PCR method helps
Other Related Services
in detection with increase specificity and sensitivity. This technology
can be employed in various testing such as: Service Page No.
• Forensic Stain Identification DNA Microarrays 35
• Forensic DNA/ RNA Quantification RT-PCR Data Analysis 81
• Human Identity Testing High-end Technology Training 88
• STR Profiling
References
1. Trisha L. Noreault-Conti, Ph.D., and Eric Buel, Ph.D. Vermont
Forensic Laboratory, Department of Public Safety, March 2007.
2. Nicklas JA, Buel E. Anal Bioanal Chem. 2003 Aug; 376(8):1160-
7. Epub 2003 May 9.
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Next Gen Sequencing
Next Generation Sequencing is changing the genetics landscape RNA-sequencing can be combined with Paired-Ends for sequencing
and opening new avenues to expand research and development. ranscriptomes from non-model organisms for which no reference
We offer customized solutions for a full range of next Gen is available. Even if you do not need the expression data, RNA-
sequencing applications such as : sequencing can also be a more cost effective strategy for
• Whole genome sequencing sequencing the gene space of an organism not previously
GENOMICS
• mRNA/Transcriptome sequencing sequenced.
• ChIP sequencing
• miRNA and Small RNA discovery RNA-sequencing profiles are also more versatile than microarray
• DeNovo Sequencing expression profiles as they are amazingly reproducible and can
• SNP Discovery represent an absolute rather than relative quantitation. In this way,
• Sequence capture data sets produced over many runs can be easily compared. Further,
• Targeted Resequencing it is possible to make gene-to-gene comparisons instead of only
• Multiplexing sequencing treatment-to-treatment comparisons of the same gene given by a
microarray.
Today’s scientific evnvironment, both commercial and academic,
necessitates moving beyond studying markers and genes to full iLife can provide Next Gen Sequencing service with
genome, transcriptome and epigenome characterization. Next Gen just an input of 10 micrograms of total RNA
even enables previously impractical experiments such as
environmental, population and metagenomics.
Service Information
While this technology offers new promise to life science and medical
researchers, it also creates complex challenges in data analysis.
We offer excellent bioinformatics support in analyzing terabytes of
data and providing satisfactory and publishable results.
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Next Gen Sequencing
ChIP sequencing Micro RNA Analysis
Unlike the ChIP-on-chip microarrays here is a technology that MicroRNA sequencing is performed for the Discovery & quantitation
involves Discovery & quantitation of protein-DNA interactions by of novel microRNAs and isoforms by sequencing cDNAs of
sequencing DNA from immunoprecipitations. microRNAs isolated from total RNA.
GENOMICS
If the DNA is recovered by immunoprecipitation, we can sequence Similar to RNA-sequencing, microRNA sequencing samples
it! We offer sequencing IP products from transcription factors, quantitatively from the entire small RNA population of the cell,
histone modifications, and RNA binding-proteins. including most RNAs smaller than tRNAs. From this data we can
simultaneously discover the expressed microRNA population of
Using specific antibodies, ChIP-sequence protocols call for the the sample as well as build an expression profile that can be
cross-linking of DNA/RNA with neighboring proteins, sonicating the compared to other samples.
fragments until they are very small (~200bp), and then purifying
the DNA/RNA. We will need just 20 micrograms of total RNA and the following
steps of microRNAs extraction, cDNA preparation, library
construction, sequencing and analysis will be performed by us.
In this way we can define the actual binding locations of proteins
genome-wide. Alternatively, we can provide whole-epigenome
profiling by sequencing the ChIP products of one or many histone
Service Information
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Next Gen Sequencing
SureSelect Target Enrichment System
(Illumina Single-End Sequencing Platform Library Prep)
Ordering Information
SureSelect Target Enrichment System significantly improves the
GENOMICS
Service No. Description
cost- and process-efficiency of next-generation sequencing. Based
on an extremely efficient hybrid selection technique, the SureSelect GST001 Customized SureSelect Target Enrichment System
Target Enrichment System can be automated and easily scaled to
meet the needs of larger sequencing projects. This is unlike any For all prices contact : customersupport@ilifediscoveries.com
other commercially available target enrichment method. With
sample input requirements at or below 3 µg of gDNA, researchers
can perform highly targeted next-generation sequencing on the most
precious of samples.
SureSelect Target Enrichment System Workflow
SureSelect platform currently supports target enrichment on both Other Related Services
the Illumina Genome Analyzer and the Applied Biosystems™ SOLiD
Service Page No.
System. Protocols and kits have been optimized for in-solution works
that are custom designed, along with a catalog product targeting DNA Extraction & Purification 20
RNA Extraction & Purification 20
human exome.
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DNA Analysis 21
RNA Analysis 22
The platform currently consists of the following offerings:
1. SureSelect Target Enrichment System: A custom, in-
solution method useful for large, higher throughput studies.
2. SureSelect All Exon Kits: A single tube assay that targets all
human exons with significant depth of coverage. 1.22% of human
genomic regions are covered, corresponding to the CCDS exons.
3. SureSelect All Exon PLUS Kits: Now you can add your own
custom content! The design covers 38Mb (CCDS + >1,000 ncRNA)
and you can add up to 6Mb of your own content
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Gene Synthesis
Why use gene synthesis instead of PCR cloning? Ordering Information
PCR cloning, the most common method of obtaining genes, may
not be able to produce a gene of sufficient quality or even at all. Service No. Description
First, a cDNA library for a specific type of tissue has to be prepared GGS001 Eukarotic gene synthesis (including codon
or purchased, which requires either time or expense. Second, the optimization; standard delivery ~4µg)
GENOMICS
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Bacterial Identification
Bacterial Species identification is challenging because of the huge
diversity of morphologically complicated organisms. 16S rRNA
sequencing is one of the most accurate and validated methods for
taxonomical identification of species.
GENOMICS
We provide services for complete workflow starting from DNA
isolation to 16S rRNA sequencing and in-silico testing services for
taxonomical identification.
Service Information
Ordering Information
Service No. Description
GPD001 Primer designing, synthesis and optimization
GBI001 Bacterial Identification without cloning
GBI002 Bacterial Identification with cloning
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PROTEOMICS & CELL BIOLOGY
Service Information
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PROTEOMICS & CELL BIOLOGY
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Proteomics &
Cell Biology
Simple Solutions-Complex Proteins
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PROTEOMICS & CELL BIOLOGY Protein Analysis
Protein Extraction & Purification His-tag protein purification with Ni++ ZipTips® Enzymatic removal
of His Tags from recombinant proteins Complementing RP-HPLC
protein purification
It is a difficult task to extract Proteins in good amount, from many
tissues. iLife offers to extract & purify highly pure protein in small
Antibody Analysis
and large-scale. We have been routinely extracting considerable
Analysis of antibodies under reducing and non-reducing conditions
amount of protein, for various proteomics applications, from various
Quantitation of the half-antibody content in IgG4 preparations
sources including tissue (fresh and preserved), blood, saliva, other
Comparison of SDS-PAGE, CGE and Agilent 2100 bioanalyzer for
body fluids, cell cultures, plants, and forensics samples.
humanized monoclonal antibody analysis Absolute quantitation of
IgG Quality control of stressed antibodies Separation of bispecific
Protein Analysis antibodies chains
Agilent 2100 Bioanalyzer is used which is a microfluidics based
platform for sizing, quantification and quality control of Proteins. Food Analysis
For detailed technical information kindly refer to the page no:14. Bovine milk analysis Protein pattern of different transgenic seedlines
Different kits allow Protein quality control – detection at ng & pg Protein - Others
level: Absolute protein quantitation Glycoprotein sizing Protein quality
control prior to MS-analysis Depletion of high abundant proteins
Protein 250 kit: from blood samples Increased sensitivity by desalting protein
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Protein Expression & Purification
Service Information
iLife can help you select expression system and strains, develop At iLife we:
standard protocols, and scale up processes for expression of • Will work with your specifications for quantity, varying size,
proteins from: bioactivity, structure, solubility, post-translational modifications,
compartmentalization (cytoplasmic/periplasmic) and purity, to
Bacterial protein expression systems: They are both the most express proteins that meet your specialized requirements.
universal and the most cost-effective means of protein expression • Will also do small scale induction to over-express target protein
available now-a-days. & carry confirmation test for expressed protein by SDS-PAGE
and western blot (customer must provide appropriate antibodies).
Yeast Expression System: This system is extremely useful for • Amplify/isolate gene of interest out of a customer-supplied vector
expression and analysis of eukaryotic proteins. Protein expression and subclone it into a suitable promoter based high-level
in yeast is genetically well characterized and is known to perform expression vector or a retroviral vector.
many post-translational modifications, grow quickly in defined
medium. It is easy and less expensive to work with, as compared Deliverables :
to insect or mammalian cells & can be adapted to fermentation. 1. Construct(s) report
2. Expression data
Mammalian Expression Systems: These systems ensure 3. Expression positive clones (glycerol stock)
appropriate post-translational modifications (e.g. disulfide bonds, 4. SDS-PAGE
glycosylation, phosphorylation) in case of a mammalian protein and 5. Western Blot data
are therefore, the preferred exression systems in case of mammalian 6. QC data at each step
proteins. It is also required for therapeutics & vaccines production, 7. Project report
hybridoma screening etc.
Ordering Information :
Service No. Description
PEP001 Expression and Purification of Protein from Bacteria
PEP002 Expression and Purification of Protein from Yeast
PEP003 Expression and Purification of Protein from Mammalian cells
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PROTEOMICS & CELL BIOLOGY Protein Expression & Purification
Epigenetic expression of proteins under Small Scale protein purification for proof
inducible promoters of concept studies
It is quite difficult to express toxic proteins, as they tend to destroy iLife Discoveries is a final solution for your needs, and helps you to
the cell producing them. iLife can help you select the inducible overcome your cloning, protein expression and purification
expression system and strains, develop standard protocols, and bottleneck We offer protein purification services for cDNA cloned
scale up processes for expression of proteins from: into bacterial, mammalian or insect cells. Recombinant protein
production is a core activity of groups involved in high throughput
1. Bacterial protein expression systems screening functional proteins for validation and structural biology.
2. Yeast Expression System The key to achieving efficient and robust purification procedures is
3. Mammalian Expression Systems selection of the most appropriate chromatographic resins for each
separation step comprising a multidimensional process, the
At iLife we sequence of which is critical for optimization of chromatographic
• Besides working on your specifications for the quantity, varying performance in terms of simplicity, scalability and product yield
size, bioactivity, structure, solubility, post-translational
modifications, compartmentalization (cytoplasmic/periplasmic) At iLife we
and purity, to express proteins, we will also work with choice of • Will work with your specifications for quantity, varying size,
inducible vector that meet your specialized requirements for even bioactivity, structure, solubility, post-translational modifications,
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Ordering Information
Service No. Description
PEP004 Epigenetic Expression of Protein under inducible
promoters from Bacteria
PEP005 Epigenetic Expression of Protein under inducible
promoters from Yeast
PEP006 Epigenetic Expression of Protein under inducible
promoters from Mammalian cells
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Protein Expression & Purification
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expression vector or a retroviral vector.
Deliverables:
1. Construct(s) report
2. Expression data
3. Expression positive clones (glycerol stock)
4. SDS-PAGE
5. Western Blot data
6. Purified soluble tagged/untagged protein in lyophilized form
7. QC data at each step
8. Project report
Ordering Information
Service No. Description
PEP010 Purification of 15-20mg protein from Bacteria
PEP011 Purification of 15-20mg protein from Yeast
PEP012 Purification of 15-20mg protein from Mammalian cells
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PROTEOMICS & CELL BIOLOGY Protein Profiling
Two - Dimensional Gel Electrophoresis, Offgel Fractionation
followed by MALDI-TOF/TOF
2D-GE is a powerful method, but in case of complex protein
2DGel electrophoresis is the most widely accepted method for mixtures, many times, people are unable to get reproducible
fractionation of complex mixture of proteins into discrete fractionation with high resolution. In contrast to conventional
components. iLife Discoveries offers high-throughput, two- isoelectric focusing, OFFGEL fractionation delivers sample in
dimensional gel electrophoresis for a diverse range of sample types. the liquid phase which is much more convenient for the other
One may like to do 2D gel electrophoresis in various studies like: subsequent separation experiments like liquid chromatography.
The main advantages are that there is no need for gel staining
• Protein Biomarker discovery and no need to recover sample from the gel, which decreases
• Protein species and isoform distribution analysis the loss of sample and increases the reproducibility of the results.
• Analysis of posttranslational modifications The OFFGEL electrophoresis reduces sample complexity,
• Differential protein pattern analysis making it possible to detect a small difference in the protein
• Drug mechanism of action pattern.
• Predictive toxicology
Deliverables :
• Project Report
Ordering Information
Service No. Description
POG001 2D Gel Electrophoresis of Bacterial Protein (Low Resolution)
POG002 2D Gel Electrophoresis of Bacterial Protein (High Resolution)
POG003 IEF OffGel Fractionation, for Differential Protein Expression
(Low Resolution)
POG004 IEF OffGel Fractionation, for Differential Protein Expression
(High Resolution)
POG005 IEF OffGel Fractionation, with Digital Analysis of Protein Fractions
(Low Resolution)
POG006 IEF OffGel Fractionation, with Digital Analysis of Protein
Fractions (High Resolution)
POG007 Customized 2D Gel Electrophoresis Services
POG008 Customized OffGel Fractionation Services
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Protein Profiling
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• Time course studies
• Biomarker discovery
• Target validation
• Target Identification
• Serum Analysis in Human, Mouse & Rat Tissues
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PROTEOMICS & CELL BIOLOGY Protein Profiling
Data Validation
The purpose of the antibody array is to provide a reliable, easy-to-
use tool that generates reproducible results. Antibody array results
can be validated by Western blot analysis, ELISA,
immunohistochemistry, or literature searches. The Western blot
analysis and array data show an 80% or better correlation..
Data Analysis
Customized data analysis will include identifying the candidate
antibodies showing differential expression and categorizing them
depending on their function.
Ordering Information
Service No. Description
PAM001 Protein Profiling on Antibody Microarray 500
PAM002 Custom Antibody Microarray
PAM003 Custom Reverse Phase Microarray
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Protein Profiling
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PPP018 Neuroscience
• Cell Cycle PPP019 NF/KB II Signalling
• Hormones PPP020 p53 signalling
PPP021 TGF/beta signalling
• Stem Cell etc. PPP022 Tyrosine Kinase Adaptors
Pathway Antibody Microarrays
Proteins from cell extracts, fresh, frozen and FFPE tissues or serum PPP023 Angiogenesis
samples can be analyzed at our facility with utmost accuracy and PPP024 Cancer Markers
deliver publication ready results. PPP025 Cytokines
PPP026 Signla Transduction
PPP027 Apoptosis
Data Analysis PPP028 Cell Cycle
Customized data analysis will include identifying the candidate PPP029 Hormones
antibodies showing differential expression and categorizing them PPP030 Stem Cells
depending on their function.
For all prices contact : customersupport@ilifediscoveries.com
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PROTEOMICS & CELL BIOLOGY Immunological assays
ELISA ELISPOT
The Enzyme Linked Immunosorbent Assay (ELISA) is a common The Enzyme-linked Immunosorbent SPOT (ELISPOT) is unlike
assay used to check the presence of particular antigens or ELISA where secreted analyte from stimulated cell is captured by
antibodies. labeled antibody for assay.
We offer a wide range of ELISA services which include: Immune cells after interacting antigens secrete number of
• Broad range screening of cytokines, antigens, signaling stimulating chemicals like cytokines. The ability to measure this
molecules, etc. response is vital for drug discovery, basic research and
• Test of various disease related antigens and plant molecules. management of a range of important diseases. This response
• Designed or synthesized peptides/ antigens which can be readout at single-cell resolution is required to develop better
screened against wide range of antibodies understanding and treatment of allergies and autoimmunity, to
predict allograft rejection in organ transplantation, for improvements
Deliverables : in drug and vaccine development, and for monitoring various cancer
1. Project report treatments.
Deliverables :
1. Project report
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Biomolecular Interaction Assays
1. Antibody-antigen
2. Receptor – Ligand
3. DNA - DNA
4. DNA - Protein
5. Antibody Fragment - Antigen
6. Antibody - Peptide
7. Multiple Antibody Pairings
8. Antibody - Small Molecule
9. Protein - Small Molecule
Figure 3: Analysis of a high affinity interaction (KD= 39 nM): acetazolamide/carbonic
Monitoring antibody concentration anhydrase binding data at 1- 0.012 ìM acetazolamide (3X dilution series) in PBS
iLife is a leading provider to the research and Industrial community with 0.5% DMSO.
of a high-end technology platform for monitoring antibody titres. 1. Papalia et al, Analytical Biochem 359 (2006), 94-105.
On receiving your established hybridomas, we continue to grow 2. Myszka, Analytical Biochem 329 (2004), 316-323.
them in our cell culture facility and monitor the titer values on a
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regular basis on the Octet Red. Conditions of growth can be altered
as required to be able to achieve larger antibody titre values. Kinetic profiling of immunotherapeutics
Immunotherapeutic agents use or modify immune mechanisms.
Use of these agents is rapidly growing. New classes, new agents,
and new uses of current agents are constantly in the development
pipeline. We can customize experimental protocols to measure on
and off rates of immunotherapeutics. Eight samples can be analyzed
in parallel.
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PROTEOMICS & CELL BIOLOGY Biomolecular Interaction Assays
Protein Quantitation in Bioprocessing Kinetic Characterization of Proteins
Our Technologies provide researchers with label-free solution for Kinetic analysis of antibodies and other proteins is critical to both
antibody quantitation. Pre-coated anti-hIgG, anti-murine, and Protein the selection of clones for development and comprehensive kinetic
A Biosensors are ready to use in quantifying a variety of IgG characterization throughout development and production. Using
isotypes. Streptavidin-coated and Amine Reactive Biosensors Octet Red we provide a unique approach in label-free, real-time
expand your quantitation options to include all proteins in developing kinetic analysis streamlining assay development. As a result ka, kd,
custom protein assays. We guarantee accurate results on and KD.
customized workflows resulting on good quality publishable data.
Kinetic Characterization Data
Ordering Information
Service No. Description
PBI006 Biomolecular interaction assay for Kinetic
characterization of proteins
Ordering Information
Service No. Description
PBI005 Biomolecular interaction assay for Protein Quantitation
in Bioprocessing
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Biomolecular Interaction Assays
Immunogenicity is the degree of the immune response to a Using the biosensor surface within this bio-layer-interferometry
therapeutic drug. Anti-drug antibodies (ADA) produced in the technology platform, it is possible to conduct ligand inhibition assays
process can elicit allergic or anaphylactic reactions, reduction in by expanding the variables that can be measured, such as blocking
efficacy, and/or induction of autoimmunity. Currently a prerequisite or unblocking analytes, affinity, and association and dissociation
for the approval of a therapeutic protein involves strict regulatory rates. Hence screening of large number of drug inhibitors can be
immunogenicity testing of biopharmaceuticals during clinical trials. carried out resulting in the creation of a drug blocking profile.
Using this technology platform we offer the pharmaceutical industry
accelerated detection of anti-drug antibodies (ADA) and
characterization of an anti-drug antibody response.
Service Information
Ordering Information Ordering Information
Service No. Description Service No. Description
PBI007 Biomolecular interaction assay for Detection PBI008 Biomolecular interaction assay for Screen
of anti-drug antibodies (ADA) for drug inhibitors
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PROTEOMICS & CELL BIOLOGY Antibody Production
Monoclonal Antibody Production Polyclonal Antibody Production
iLife provides our customers a very comprehensive expertise in We provide a flexible approach for polyclonal antibody development
the area of Molecular Biology and Immunology giving end-to-end against protein/peptide of choice. The basic service package
solutions for Recombinant Protein and Custom Antibody comprises of antisera development which can be extended to
development. purification trial and final purification which is briefed as follows:
Ordering Information
Service No. Description
PAD001 Monoclonal Antibody production
PAD002 Polyclonal Antibody Production
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Peptide Synthesis Metabolite Analysis
Service Information
All the peptides will be delivered with a Certificate of Analysis
specifying the HPLC gradient used to determine peptide purity. • Nutrient profiling
• Amino acid profiling
We specialize in supplying 20 to 1000 peptides with lengths up to • Water soluble vitamins by HPLC
15 residues and quantities up to 30 mg. We also offer ~1mg of • Fat soluble vitamins by HPLC
peptide on 96 well plates. For large peptide synthesis projects, we • Food additives
also offer peptide pooling. • Acidulants
• Antioxidants
• Preservatives
• Artificial Sweeteners
• Synthetic colors
• Nutritional labeling
• Trace analysis
• Pesticide residues
• Antibiotic residues
• Dioxins
• Toxic metals
• Mycotoxins
• Epoxy resins
• Chemotherapeutics and antiparasitic agents
• Microbiological evaluations
• Shelf life studies
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PROTEOMICS & CELL BIOLOGY Metabolite Analysis
MALDI ToF/ToF Services
iLife is equipped with the next-generation tandem time-of-flight MS/
MS system - ABI4800 MALDI ToF/ToF Analyzer.
Ordering Information
Service No. Description
PMD009 Trypsinization
PMD010 Mass Analysis of Proteins
PMD011 Mass Analysis of Peptides
PMD012 Peptide Mass Fingerprinting through MALDI ToF
PMD013 MALDI ToF/ToF
PMD014 De novo sequencing by MS/MS
Ordering Information
Service No. Description For all prices contact : customersupport@ilifediscoveries.com
PHP001 Analytical HPLC
PHP002 Semi-preperative HPLC
PHP003 Perparative HPLC Other Related Services
PHP004 Customized LC-MS/MS solutions
PHP005 Customized GC-MS Service Page No.
PHP006 Customized GC-MS/MS
PHP007 Customized GC-MS/TOF Protein Extraction and Purification 56
PHP008 Customized ICP MS Protein Expression & Purification 57
2D Gel Electrophoresis 60
Offgel Fractionation 60
For all prices contact : customersupport@ilifediscoveries.com Protein Structure Prediction 84
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Customized Cell Biology Services
Service Information
Our services include
1. Design of the entire project for stable cell construction
2. cDNA synthesis to expression of the protein in a mammalian
cell compatible vector with dual antibiotic resistance genes
3. Selection of the right cell line
4. Initial transient transfection study for the verification
5. Stable cell line transfection under CLASS-10000 clean room
conditions
6. Selection of multiple clones from the transfected population
7. Differential gene expression studies of the stable cell lines to
study the effect of over expression of gene of interest.
8. Constitutively active expression based recombinant cell lines
9. Stable cell lines with integrated inducible expression systems
for the study of toxic or pro-apoptotic genes.
We offer Continuous supply of viral particle preparations which are
ready to infect the cells of your interest once the viral particle
construction is completed, there by eliminating the need to set up
infrastructures in your laboratory.
We offer complete services ranging from design of different Novel Cell Lines Development
adenoviral expression platforms. Our custom services include the As an innovative new addition to our
overall evaluation of project, analysis of the primary and/or capabilities, our cell biology team can
transformed cell lines for which the expression systems are created develop novel cell lines from a variety
etc. We internally design appropriate primers for amplifying the of mammalian and non mammalian
ORF (Open Reading Frame), construction of wild type ORF with sources. We are currently developing
N- or C- terminal tags for sub-cellular localization and/or purification, cell lines of pharmaceutical relevance.
creation of library of mutant proteins etc prior to their integration These cell lines are derived from
into the appropriate adenoviral backbone. Indian population with different
Small or big ORFs: Expression in hard to transfect cells lines; cultural and dietary practices.
Expression for large scale purification of proteins; Expression for
studying sub-cellular localization, adenovirus are the most accepted
ways for expression recombinant proteins with highest efficiency Ordering Information
with least undesirable effects.
Our adenoviral expression services are performed on a customized Service No. Description
project format. PCB001 Adenovirus Services
PCB002 Generation of Stably Expressing Cell Lines
PCB003 Novel Cell Lines Development
PCB004 Other Customized Cell Biology Services
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Service Information
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Bioinformatics
Simplify Complex Life Research
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Bioinformatics Expertise
Introduction and Applications
Bioinformatics or computational biology is the use of mathematical Pharmacogenomics
and informational techniques, including statistics, to solve biological Pharmacogenomics is the application of genomic approaches and
BIOINFORMATICS
problems, usually by creating or using computer programs, technologies to the identification of drug targets. In Short,
mathematical models or both. One of the main areas of pharmacogenomics is using genetic information to predict whether
bioinformatics is the data mining and analysis of the data gathered a drug will help make a patient well or sick. It Studies how genes
by the various genome projects. Other areas are sequence influence the response of humans to drugs, from the population to
alignment, protein structure prediction, systems biology, protein- the molecular level.
protein interactions and virtual evolution.
Pharmacogenetics
Genomics
Pharmacogenetics is the study of how the actions of and reactions
Genomics is any attempt to analyze or compare the entire genetic
to drugs vary with the patient’s genes. All individuals respond
complement of a species. It is, of course possible to compare differently to drug treatments; some positively, others with little
genomes by comparing more-or-less representative subsets of obvious change in their conditions and yet others with side effects
genes within genomes. or allergic reactions. Much of this variation is known to have a genetic
basis. Pharmacogenetics is a subset of pharmacogenomics which
Comparative genomics uses genomic/bioinformatic methods to identify genomic correlates,
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The study of human genetics by comparisons with model organisms for example SNPs (Single Nucleotide Polymorphisms),
such as mice, the fruit fly, and the bacterium E. coli. characteristic of particular patient response profiles and use those
markers to inform the administration and development of therapies.
Strikingly such approaches have been used to “resurrect” drugs
Structural genomics or structural thought previously to be ineffective, but subsequently found to work
bioinformatics with in subset of patients or in optimizing the doses of chemotherapy
This refers to the analysis of macromolecular structure particularly for particular patients.
proteins, using computational tools and theoretical frameworks.
One of the goals of structural genomics is the extension of idea of Cheminformatics
genomics, to obtain accurate three-dimensional structural models The mixing of those information resources to transform data into
for all known protein families, protein domains or protein folds. information and information into knowledge for the intended purpose
Structural alignment is a tool of structural genomics. of making better decisions faster in the arena of drug lead
identification and optimization. Related terms of Cheminformatics
Functional genomics are chemi-informatics, chemometrics, computational chemistry,
chemical informatics, chemical information management/science,
Functional genomics is a field of molecular biology that is attempting and Cheminformatics.
to make use of the vast wealth of data produced by genome
sequencing projects to describe genome function. Functional Biomedical Informatics / Medical Informatics
genomics uses high-throuput techniques like DNA microarrays,
proteomics, metabolomics and mutation analysis to describe the Biomedical Informatics is an emerging discipline that has been
function and interactions of genes. defined as the study, invention, and implementation of structures
and algorithms to improve communication, understanding and
Proteomics management of medical information.
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Bioinformatics Expertise
In silico Drug Designing Agroinformatics / Agricultural Informatics
Drug discovery is a complex and risky treasure hunt to find the Agroinformatics concentrates on the aspects of bioinformatics
most efficacious molecule which do not have toxic effects but at dealing with study of plant genomes. Microarray techniques to study
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the same time have desired pharmacokinetic profile. The hunt starts plant disease and causing factor for it due to which genes are
when the researchers look for the binding affinity of the molecule suppressed or over expressed. Comparative genome analysis of
to its target. Huge amount of research requires to be done to come different species of plants.
out with a molecule which has the reliable binding profile. Once the
molecules have been identified, as per the traditional methodologies, Systems Biology
the molecule is further subjected to optimization with the aim of
improving efficacy. The molecules which show better binding are Systems biology is the coordinated study of biological systems by
then evaluated for its toxicity and pharmacokinetic profiles. It is at investigating the components of cellular networks and their
this stage that most of the candidates fail in the race to become a interactions, by applying experimental high-throughput and whole-
successful drug. Bioinformatics make it easier to design successful genome techniques, and integrating computational methods with
drug with the help of bioinformatics tools and also saves time and experimental efforts.
money.
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Microarray Data Analysis
Microarray data sets are very large and complex. Statistical challenges include taking into account effects of background noise and
appropriate normalization of the data. Normalization methods are different for different platforms.
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Data Processing
This include background subtraction (global or local background), determination of spot intensities and intensity ratios, visualization of
data in different plots and log-transformation of ratios, global or local normalization of signal intensity values.
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Microarray Data Analysis
Single Color and Two Color Gene Expression Data Analysis
A gene expression microarray is a technology used in genomics to Custom designed array etc. Please contact us and ask what you
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know the change in expression of gene. microarray has thousands could get from your experimental data after analysis.
of spots of DNA oligonucleotides, that is called features. Each
feature contains several probes of a specific DNA sequence of Basic Analysis
particular gene. Probes hybridize to cDNA or cRNA sample and is • Data processing
detected and quantified to determine relative abundance of cDNA • Normalization
or cRNA in the sample. One micro array chip contains thousands • Experiment Grouping
of probes, a microarray experiment can perform many genetic tests • Quality control on samples
in parallel. This is why microarray is the first choice of any genetic • Quality control on probes
research. • Statistical Analysis
Due to biological complexity of gene expression, data analysis plays • Differentially expressed significant genes
major role to find out differentially expressed genes. To begin with • Fold change analysis
data analysis, experimental design is very critical and important if • Clustering of genes
statistically and biologically significant conclusions are to be drawn • PCA Analysis
from the data. Hence at least two biological or technical replicates
are important for analysis. Advance Analysis
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• GO Analysis
Under this section we can analyze any platform based gene • GSEA
expression data analysis such as Agilent single color and two color, • Class prediction
Affymetrix single color, Illumina single color and two color, • Pathway Analysis
Nimblegen single color, GE single color, combimetrix single color
and all kinds of custom array for single color and two color.
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Microarray Data Analysis
Exon Expression and Exon Splicing Data Analysis
Alternative splicing is defined as variations in RNA splicing Advance Analysis
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Basic Analysis
• Data processing
• Normalization
• Experiment Grouping
• Quality control on samples
• Quality control on probes
• Statistical analysis
• Differentially expressed significant genes
• Filter Transcripts on DABG
• Splicing ANOVA
• Filter on Splicing Index
• Splicing Visualizations
• Profile Plot
• Variance Plot
• Fold change analysis
• Clustering of genes
• PCA analysis
Ordering Information
Service No. Description
BEE001 Exon Expression Basic Data Analysis
BEE002 Exon Expression Advance Data Analysis
BES003 Exon Splicing Basic Data Analysis
BES004 Exon Splicing Advance Data Analysis
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Microarray Data Analysis
Copy Number Analysis Association Data Analysis
Copy number analysis is important to understand or to explore the Genome wide association study (GWAS) is extremely significant
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effect of copy number variation in human disease and point method to identify susceptible genes involved in common human
mutation. Ideally there would be two copies of gene in human diseases and increases chance to find targets for therapeutic
genome but practically we see that there is several copy of same intervention that act on the root cause of disease.
gene present in genome which trigger disease or prevent disease This analysis requires thousands of case control sample to find
in human. Several thousands of SNPs can be estimated for the statistically significant loci which occurs due to difference in allele
copy number variation and genomic position. or genotype frequency in case with respect to control. The difference
in allele or genotype frequency can lead to disease and thus this
This analysis includes Copy Number Variants , Copy Number method is useful in disease gene discovery due to unbiased study
Polymorphism, Log Ratio, Loss in Heterozygosity (LOH), of gene with known or suspected disease mechanism.
cytogenetic analysis, genotyping calls, copy number calls for CNV
regions and individual probe sets, cluster graphs, Reference analysis Analysis includes
against control, Allele Specific Copy Number, Parent specific Copy • Quality control on samples
Number, Genomic Identification of Significant Targets in Cancer • Filter to get significant SNP
(GISTIC) etc can be analyzed. • PCA
• Statistical analysis
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Copy number analysis • Pearson’s chi square test
• Quality control on samples • Fisher’s exact test
• Batch Effect Correction • Cochran-Armitage test
• Statistical t-test • Regression analysis
• PCA • Linkage Disequilibrium (LD) analysis
• Mean log ratio
• Filtering
• GISTIC
• Clustering
• GO analysis
Ordering Information
Service No. Description
BCN001 Copy number Analysis
BAA002 Association Data Analysis
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Microarray Data Analysis
miRNA Data Analysis Custom Chip Design and Analysis
miRNA is small single-stranded, regulatory RNA molecules of To study gene expression at genomic scale mostly microarray chips
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approximately 25 nuceotide base long molecule. miRNA regulates are, it is important to design oligo probes that specifically represent
mRNA by binding with complementary sequences of mRNA and large number of DNA sequences. Cross-hybridization is most likely
affects the expression of genes involved in several biological from the regions with contiguously and perfectly matched bases.
processes. Therefore miRNA expression study became important Oligo probes should also be sensitive and hybridize under similar
class to understand the role of miRNA in regulating biological conditions. We design chip for diagnostic purposes by picking
processes. miRNA array developed to study expression patterns specific oligos by skipping regions with contiguous bases common
of miRNA for any conditions and can be correlated with their target in other sequences. We assure for specificity and it is double-
gene expression studies. checked by NCBI BLAST. Similar sequence regions to non-coding
RNAs are avoided because total RNA are often used for array
hybridization. Low-complexity regions are also filtered out to
Basic Analysis maintain oligo specificity. Oligos and sequence regions that may
• Data processing form secondary structures are discarded since both the probes and
• Normalization the sequence target sites should be easily accessible for
• Experiment Grouping hybridization.
• Quality control on samples
• Quality control on probes
Parameters are taken in consideration while primer
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• Statistical analysis
• Differentially expressed significant genes designing:
• Fold change analysis Probe Copy, Probe Length, Tm range, Cross-reactivity, BLAST
• Clustering of genes score, End preference, Filter file, Cross-reactivity to filter sequences,
• PCA analysis Cross-reacting probes.
We provide services for custom chip design for microarray
Advance Analysis experiemnt. Custom chip design could be done on user’s
• Find target gene (Target Scan) preferences. Selected probes or genes of user’s interest can be
• GO analysis spotted on glass slide to carry out higher level of research.
• GSEA This is also like another microarray chip but only difference would
• Class prediction be in quantity and specificity of genes, for certain experiment.
• Pathway analysis Analysis of data would be same as per other microarray experiment.
Basic Analysis
• Normalization
• Experiment Grouping
• Quality control on samples
• Quality control on probes
• Statistical analysis
• Differentially expressed
significant genes
• Fold change analysis
• Clustering of genes
• PCA analysis
Advance Analysis
• GO analysis
• GSEA
• Class prediction
• Pathway analysis
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
80 (US, Canada & Europe) Call +1 408 - 907 6645
RT PCR Data Analysis
Real Time PCR is used to measure the quantity of DNA, cDNA, or
RNA present in a sample. It is extremely sensitive technique for
mRNA detection and quantization. RT-PCR can be used to quantify
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mRNA levels from very smaller samples. Therefore, this technique
is very sensitive to enable quantitation of RNA from a single cell. A
DNA-binding dye binds to all double-stranded DNA in PCR that
causes fluorescence. An increase in DNA product during PCR leads
to an increase in fluorescence intensity and is measured at each
cycle, this allows DNA concentrations to be quantified. However,
DNA dyes bind to all DNA PCR products, including nonspecific
PCR products like Primer, dimer. This can potentially interfere with
or prevent accurate quantification of the intended target sequence.
Basic Analysis
• Data processing
• Normalization
• Experiment Grouping
• Quality control on samples
• Quality control on probes
Service Information
• Statistical analysis
• Differentially expressed significant genes
• Fold change analysis
• Clustering of genes
• PCA analysis
Advance Analysis
• GO analysis
• GSEA
• Class prediction
• Pathway analysis
Ordering Information
Service No. Description
BRT001 RT PCR Basic Data Analysis
BRT002 RT PCR Advance Data Analysis
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
(US, Canada & Europe) Call +1 408 - 907 6645 81
Comparative Genomics
The comparative genome analysis is done to establish the
correspondence between genes or other genomic features in
different organisms. We can create intergenomic maps that make
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Sequence Analysis
• Determination genes that encode proteins, RNA, regulatory
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Evolutionary Studies
• Tracing the evolution of an organism based on the changes in
its DNA contents.
• Construction of complex evolutionary computational models.
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
82 (US, Canada & Europe) Call +1 408 - 907 6645
Comparative Genomics
Primer Designing
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biotechnology field. This technology is used for fast mass duplication
of DNA sequences. By repeating some cycles of the denaturation,
annealing and extension reactions, it allows a small amount of DNA
to be amplified exponentially. Typically 25–45 of these cycles are
performed. Amplification of specific regions of the genome is
determined by specific primer sets with forward and reverse
orientation. Typically considered primer design constraints are the
length of the primer, the GC content, the melting temperature and
GC clamp. The length of primers should be within 16 bps~28 bps,
while the difference of the primer pair length should not exceed 3
bps. The GC content of primers should be within 40%~60%. The
melting temperature of primers should be within the range of
o o
50 C~62 C, while the difference of the melting temperature of a
o
primer pair should not exceed more than 5 C. Finally, the 3’ end of
primers should be G or C.In recent years, secondary structures
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were applied to primer design, such as dimer, hairpin and specificity
of a primer pair. Use dynamic programming to design primers. This
allows for designing multiple primers for multiple target DNA
sequences. We identify the specificity-determining subsequence
of each primer and examine its uniqueness in the target genome.
Ordering Information
Service No. Description
BPD001 Primer Designing
BPW002 Proteomics Work in Bioinformatics
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
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Prediction of Protein Structure
Protein-Protein Interaction Studies Peptide Library Synthesis
Protein is made up of amino acids and the information for protein We could generate peptide of your interest from the given protein.
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synthesis (primary structure) during translation comes from codon. We could help you in peptide array design to study the specific
Codon is the three nucleotide sequence on gene that is why we protein of your interest. Peptide library is a systematic combination
could determine primary structure of protein from the gene of any of different peptides in large number. It is widely used in :
species. It is vital to know the structure of protein (secondary, tertiary • Drug screening
and quaternary) which may cause disease or prevent the disease. • Target validation
• Epitope mapping
Bioinformatics is used for the study of homology modeling to predict • Vaccine development
the function of a gene with unknown function. if a gene X with • Peptide biological assay
known function is homologous to the sequence of gene Y whose • Protein-peptide binding assay
function is not known, but we could conclude that while gene X and • Immune monitoring
Gene Y share same genetic information then function of gene Y • Structure-activity studies.
could be the same as function of gene X. In the structural
bioinformatics, homology is used to determine which parts of a
protein are important in structure formation and interaction with
other proteins and this technique is called homology modeling.
Service Information
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
84 (US, Canada & Europe) Call +1 408 - 907 6645
Antibody Microarray Data Analysis
The advanced step of microarray technique wherein researchers
concentrate on small number of proteins and their role in biological
system. Antibody microarray technique is used to discover and
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validate protein biomarkers in different samples. This is also used
as screening techinique for biomarker discovery and rapid
expression profiling of proteins.
Service Information
• Statistical analysis
• Fold change analysis
• Clustering analysis
• PCA analysis
• Data visualization in different plot
• Scatter plot
• Profile plot
• Box whisker plot Ordering Information
• Histogram
Service No. Description
• MvA plot
BAM001 Data Analysis for antibody microarray 500
• PCA plot BAM002 Data Analysis for pathway focused antibody
microarray
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
(US, Canada & Europe) Call +1 408 - 907 6645 85
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Shipment Guidelines
For Protein Applications
Sample Type Sample Shipment Guidelines
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
86 (US, Canada & Europe) Call +1 408 - 907 6645
Terms & Conditions Ordering Information
PRICES U.S., CANADA & EUROPE ORDERING
• Please contact our Customer Support Team
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(customersupport@ilifediscoveries.com) to obtain a quote for By Mail: Imperial Life Sciences USA, Inc.
the desired published services. 1630-1634, Centre Pointe Drive, Milipitas
• Please provide the Service no. and Quantity of the samples for CA, 95035- USA
desired services, when contacting our Customer Support Team.
• Customized quotes can be provided based on various factors By Telephone: 9:30 AM to 7:00 PM IST
+1 408- 907 6645
such as nature & duration of the project. Please contact our
Customer Support Team (customerupport@ilifediscoveries.com)
By Email: customersupport@ilifediscoveries.com
for customized quotes.
NOTE: All customized projects need additional background By Internet: http://www.ilifediscoveries.com
research by pour scientific team which may result into an
additional time to submit a quotation. Currency: US Dollars ($)
• All accounts with Annual Rate Contract with iLife Discoveries
to be exercised with Price list under Rate Contract. Please
contact for more details. ASIA ORDERING
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SHIPPING
INDIA
• An additional handling charge of INR1000.00 per order is
By Mail: iLife Discoveries
applicable.
Plot No. 154 S, Sector- 7
• An additional charge of INR 1200.00 per shipment is applicable IMT Manesar, Gurgaon
for shipments requiring Dry Ice. Haryana- 122 050
• Customized sample collection, shipment & handling at customer
site, anywhere in India are offered at an additional cost. Please By Telephone: 9:30 AM to 7:00 PM IST
contact our Customer Support team for details. +91 124- 4779800
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
(US, Canada & Europe) Call +1 408 - 907 6645 87
High-end Technology Training
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Ordering Information
Service Information
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
88 (US, Canada & Europe) Call +1 408 - 907 6645
forteBio Octet Technology Training
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Training on Octet includes
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Introduction to Biolayer Interferometry Service No. Description
technology and Instrumentation PHT001 ForteBio Octet Technology Trainig (Academia)
Protein Quantitation studies PHT002 ForteBio Octet Technology Trainig (Corporate)
Kinetic Characterization studies PHT003 Customized Octet Technology Training (Academia)
Small molecule and small peptide studies
For all prices contact : customersupport@ilifediscoveries.com
Duration: 4 working days
Service Information
For Technical or Customer Assistance, (Asia) Call +91 124 - 477 9800
(US, Canada & Europe) Call +1 408 - 907 6645 89