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1080 A. Introduction
One of the most important aspects of analysis is preparing the Use any method to prepare reagent water that can meet the
reagent water used to prepare and dilute reagents and prepare applicable quality requirements. Various combinations of
blanks. Reagent water is water with no detectable concentration reverse osmosis, distillation, and deionization can produce
of the compound or element to be analyzed (i.e., it is below the reagent water, as can ultrafiltration and/or ultraviolet irradia-
analytical method’s detection level). Reagent water should also tion. Keep in mind, however, that improperly operated or
be free of substances that interfere with analytical methods. maintained water purification systems may add rather than
However, its overall quality (concentrations of organic, inor- remove contaminants.
ganic, and biological constituents) will depend on the water’s This section provides general guidelines for preparing reagent
intended use(s). water. Table 1080:I lists commonly available water purification
processes and the major classes of contaminants that they re-
move. For details on preparing water for microbiological tests,
* Reviewed by Standard Methods Committee, 2011. see Section 9020B.4d. T1
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REAGENT WATER (1080)/Reagent Water Quality
Distillation G–E† P G E E E
Deionization E E P P P P
Reverse osmosis G‡ P G E E E
Carbon adsorption P P§ G–E㛳 P P P
Filtration P P P E E P
Ultrafiltration P P G# E E E
Ultraviolet oxidation P P G–E** P G†† P
Permission to use this table from C3–A2, Vol. 11, No. 13, Aug. 1991, “Preparation and Testing of Reagent Water in the Clinical Laboratory—Second Edition” has been
granted by the National Committee for Clinical Laboratory Standards. The complete current standard may be obtained from National Committee for Clinical Laboratory
Standards. 771 E. Lancaster Ave., Villanova, PA 19085.
* E ⫽ Excellent (capable of complete or near total removal), G ⫽ Good (capable of removing large percentages), P ⫽ Poor (little or no removal).
† The resistivity of water purified via distillation is an order of magnitude less than that in water produced via deionization, mainly due to the presence of CO2 and sometimes
H2S, NH3, and other ionized gases (if present in feedwater).
‡ The resistivity of dissolved ionized solids in product water depends on original feedwater resistivity.
§ Activated carbon removes chlorine via adsorption.
㛳 When used with other purification processes, special grades of activated carbon and other synthetic adsorbents are excellent at removing organic contaminants. Their use,
however, is targeted toward specific compounds and applications.
# Ultrafilters reduce specific feedwater organic contaminants based on the membrane’s rated molecular weight cut-off.
** 185-nm UV oxidation (batch process) removes trace organic contaminants effectively when used as post-treatment. Feedwater makeup plays a critical role in their
performance.
†† While 254-nm UV sterilizers do not physically remove bacteria, they may have bactericidal or bacteriostatic capabilities limited by intensity, contact time, and flow rate.
reverse osmosis, or adsorption. If using commercially prepared organics in water and the adsorption process may be inade-
resin columns, follow supplier’s recommendations for monitor- quate for removing low-molecular-weight, polar compounds.
ing QC of reagent water from specific equipment. Performance differences among activated carbons are attrib-
utable to the raw materials and activation procedures. Even
4. Adsorption with an optimal activated carbon, proper performance will not
be attained unless the column is sized to provide required face
In adsorption, water is fed into a reactor filled with an velocity and residence time at the maximum process flow rate.
adsorbent material (typically, granular activated carbon, al- If using commercial sorbent systems, follow supplier’s rec-
though some resins and other manmade adsorbents are used in ommended flow and QC steps.
specific applications). Chlorine and other organic impurities Using activated carbon may adversely affect the reagent wa-
are drawn from the water to the surface of the adsorbent. How ter’s resistivity. This effect may be controlled via reverse osmo-
well the process works depends on the organic contaminants sis, mixed resins, or special adsorbents. To minimize organic
involved, the activated carbon’s physical characteristics, and contamination, use mixtures of polishing resins with special
the operating conditions. In general, organics-adsorption ef- carbons and additional treatment steps (e.g., reverse osmosis,
ficiency is inversely proportional to the solubility of the natural carbons, ultraviolet oxidation, or ultrafiltration).
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REAGENT WATER (1080)/Reagent Water Quality
TABLE 1080:II. REAGENT WATER SPECIFICATIONS High-quality water cannot be stored without degrading signif-
Quality Parameter High Medium Low
icantly. Medium-quality water may be stored, but keep storage
time to a minimum and make sure quality remains consistent
Resistivity, megohm-cm ⬎10 ⬎1 ⬎0.1 with the intended use. Only store it in materials that protect the
at 25°C water from contamination (e.g., TFE and glass for organics
Conductivity, mho/cm ⬍0.1 ⬍1 ⬍10
analysis or plastics for metals).
at 25°C
SiO2, mg/L ⬍0.05 ⬍0.1 ⬍1
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