Documente Academic
Documente Profesional
Documente Cultură
ISLAMABAD
CURRICULUM
FOR
at
Riphah International University
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BACHELOR OF SCIENCE IN MEDICAL LABORATORY TECHNOLOGY
Objectives of Program
This program will provide the knowledge and practical skill to the medical lab trainees, who would be
able to…
a. Learn and apply theory and techniques in the medical pathology laboratory.
b. Avoid problems pertaining to collecting, transporting, handling and conducting tests on
laboratory samples
c. Confidently and correctly carry out all the bench work required for diagnostic tests in various
discipline of pathology.
d. Demonstrate proper handling and preventative maintenance of instruments.
e. Convey timely laboratory results to the clinicians
f. Perform and monitor quality control in the laboratory.
g. Assist the consultants in research.
h. Demonstrate ethical behavior and decision making.
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Scheme of study& Courses-(Semester wise Breakdown)
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presentation skills (English-III)
Total 18
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Third Year Semester – 7
The course will be assessed at the end of the course completion based on practical (20%) and theory
examination (40%) as per TOS. Out of total 100 % of assessment, 40 marks will be for final theory paper
and 20 marks practical examination on relevant course.
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First Year Semester-1
Course Detail/Contents
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6. Practicals:
Study Axial and Appendicular skeleton on human skeletal model.
Study musculoskeletal system on human musculoskeletal model.
Study and understand anatomy of human body.
. Human models
.Video demonstration
7. Recommended Books:
Essentials of Anatomy and Physiology by Seely, Stephens, and Tate, 4th edition
Anatomy & Physiology by Rose & Wilson
General Anatomy by Ghulam Ahmad/ Laeeq Hussain
Anatomy by D.R Johnson & K.L Moore
Regional Anatomy by Snells.
Medical Laboratory Manual for Tropical Countries. Vol I, Second Edition by Monica
Cheesbrough
2. Integumentary System
Functions of the skin
Temperature and its regulation
3. The Musculoskeletal System:
Functional characteristics of Skeletal Muscle and Cardiac Muscle
Basic concept of events of muscle contraction and relaxation in response
to an action potential in a motor neuron.
Distinguish between aerobic and anaerobic muscle contraction.
4. Respiratory System
Mechanism of breathing
Uptake and release of oxygen in the lung and the tissue
Term used in the respiratory medicine
Respiratory failure, Anoxia and hypoxia, Cyanosis, pneumothorax, Empyema,
Pneumonia, Asthma, Bronchitis, Pulmonary tuberculosis, Lung cancer, URTI,
PUO
5. Blood
Composition of Blood and Plasma
Functions of Blood
Formed Elements
Stages of cell development
Blood grouping
Coagulation mechanism
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6. The Cardiovascular system
Functions of the Heart
Conducting system of the heart and Cardiac Cycle
Heart Sounds
Regulation of Heart Functions--- intrinsic and extrinsic
Pulmonary Circulation
Systemic Circulation: Arteries
Veins
Blood pressure
Term used in Cardiac medicine.
Angina, Atherosclerosis, Blue baby, Bradycardia, Cardiac arrest,
Cardiac (heart) failure, Electrocardiogram, Endocarditis, Heart
murmurs, Ischemic heart disease, Myocardial infarction,
Myocarditis, Tachycardia
7. Practicals:
Introduction to microscope
Bleeding time
Clotting time
WBC’s count
RBC’s count
Platelets count
Reticulocytes count
8. Recommended Books:
Essentials of Anatomy and Physiology by Seelay, Stephens and Tate. 4th edition
Rose & Wilson Anatomy and Physiology
Human Physiology. Stuart Ira Fox 7th edition
Text book of Medical Physiology Guyton
Essential of Medical Physiology Vol I & II by Mushtaq Ahmad
Medical Laboratory Manual for Tropical Countries, Vol I, Second Edition by Monica
Cheesbrough
1. Cell
Introduction of Biochemistry
Cell (biochemical aspects)
Cell membrane structure
Membrane proteins
2. Body fluids
Structure and properties of water
Weak acids and bases
Concept of pH and pK
Buffers, their mechanism of action
Body buffers
3. Carbohydrates
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Definition
Classification
Biochemical Functions & Significance of Carbohydrates
Isomers of monosaccharides
Structure & properties of Monosaccharides & Oligosaccharides
Structure & properties of Polysaccharides
4. Metabolism of Carbohydrates
Digestion & Absorption of Carbohydrates
Glycolysis & its Regulation
Citric Acid Cycle
Inter conversion of carbohydrates, lipids and proteins
Biochemistry of diabetes mellitus
Glycogenolysis
5. Lipids
Classification of Lipids
Fatty Acid: Chemistry
Structure and properties of Triacylglycerols and compound lipids
Cholesterol: Chemistry, Functions & clinical Significance
Digestion & Absorption of Lipids
Role of bile salts and bile acids
Metabolism & clinical Significance of Lipoproteins
Fatty acid oxidation biosynthesis and metabolism of Triglycerides
Metabolism & clinical Significance of Cholesterol
Ketone bodies
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Recommended Books
Structure of SI Units
Standardized Reporting of Laboratory Result
Units in Clinical Enzymology
2. Pathology Laboratory Safety Precautions
o Biological safety
o Chemical safety
o Electrical hazards
o Personal protective equipment
o Occupation safety and health hazards (OSHA)
3. Specimen collection
Types of specimens
Handling of specimens
Sources of variation in lab results
4. Reagent grade water
Water purification instruments
Type1, 2,and 3 water
Preparation of reagent grade water
Testing of water quality
5. Buffers
Type of buffers
Measurement of
Buffer PH
6. Solutions preparation
7. Laboratory Glass and Plastic Ware (Use, storage and cleaning)
Types of Glassware
Cleaning of Glassware
Pipettes
Burettes
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Test Tubes
Beakers
Flasks Cylinders
8. Basic Laboratory Equipment; Operation, calibration, maintenance
Laboratory Centrifuge
Balance
Refrigerators, Freezers And Deep Freezers
pH meter
Mixers
Incubators
Ovens
Safety cabinet
9. Basic Laboratory Procedures Practical
Calculation of the concentration of the substances determined.
Preparation of solution
Preparation of Calibration Curve
pH measurement
Use of common lab instruments like balance, water bath, centrifuge etc
PPE use
First aid
1. Introduction
Definition and importance of First Aid
Contents of first aid box in the lab
First aid charts
2. Emergency treatment of cut and bleeding
Caused by Contaminated glass ware
Bleeding from a limb
Bleeding from nose
3. Emergency treatment of burns
Heat burns
Chemical burn of skin
Chemical injury in eye
4. Emergency treatment of poisoning
Swallowing acid or alkali
Swallowing other poisonous chemicals
Swallowing of infected material
Mouth pipetting
5. Emergency treatment of Fainting
6. Emergency management of Electric shock
7. Emergency resuscitation when a person stops breathing
Mouth to mouth breathing
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8. First aid in cases where the heart stops beating
9. Code of safety for the labs
o Formulating a code of safe laboratory practice
o Enforcing a code of safe laboratory practices
10. Recommended Books:
Medical Laboratory Manual for Tropical Countries. Vol I, Second Edition by Monica
Cheesbrough
Functional English
Aims
The course aims to equip the students with fundamentals of English grammar and other
important structures
Course Objectives
By the end of the course students would be able to:
understand fundamental structures of English Grammar which includes Parts of
Speech, Tenses, Voice, Narration and punctuation
compose grammatically correct sentences and unified paragraphs
develop their written communication
Course Contents
Basics of Grammar
Parts of speech
Verbs and its Kinds
Definite and Indefinite Articles
Tenses: Present, Past and Future
Phrase, Clause and sentence structure
Variety of Sentences
Voice
Narration
Transitive
Punctuation
Infinitive Gerund and Participle
Mood
Reading Comprehension
Paragraph writing
Methodology
The course will be taught mainly through lectures, tutorials, group discussions,
simulations and student presentations.
Suggested Readings
English Grammar through stories by Alan Townend
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Made Easy Series by Afzal Anwar Mufti
o Tenses Made Easy
o Active Passive Made Easy
o Narration Made Easy
Practical English Grammar: Exercises 1 and Exercises 2
Essential English Grammar by Raymond Murphy
High School English Grammar by Wren and Martin
Course Title: Islamic Ethical Principles and Contemporary Issues (Islamic Studies)
Course Pre-requisite:
Course Description:
The first part of this course introduces and practiced the students about principles and corrects
pronunciation of Arabic Alphabets. The second part provides sufficient knowledge to fundamentals and
pillars of Islam and its adherent within contemporary global dynamics. The final part of this course deals
will some most contemporary issues, including nationalism, capitalism reformist and radical Islam.
Students will also explore the challenge of shaping a Muslim identity in the Muslim world, in the context
of key Muslim institutions and social movement. This course will be university required course and
partially fulfill the requirement of HEC Islamic studies undergraduate compulsory course.
Course Goals:
To provide the students an understanding of Islam and its adherent in the context of
contemporary global dynamic world.
To address and explore the new challenges and critical issues facing Islam in the Muslim
world.
Course Objectives:
1. General:
a. To develop ethically knowledgeable and practicing professionals
b. To prepare a good Muslim professional
c. To develop a balance dynamic and wholesome personality
2. Specific
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a. Knowledge:
i. Quran incorporation
ii. To understand the basic concept of Islamic teaching.
iii. To give the knowledge of fundamentals and pillars of Islam
iv. To understand the elements and characteristics of Islamic civilization
v. To provide the sufficient knowledge and create awareness regarding
contemporary issues.
b. Skills:
1. Interactive lectures
2. Discussions which links to theory to practice
3. Demonstration
Course Contents: (Part 1)
1. Quran Incorporate
2. Religion (Introduction, Importance, Significance)
3. Introduction to fundamentals of Islam
a. Tawhid
i. Existence of ALLAH, An overview of different approaches to the concept of
God
ii. The importance of `Aqidah Tawhid in the life of a muslim.
iii. Tawhid in the history of mankind according to the Quran.
iv. Prophet (s.a.w) role in reviving the Aqida of Tawhid.
v. Role of Tawhid in transformation human culture and civilization
vi. Effects of Tawhid on individual and collective life
b. Risalat
i. Importance and Significance
ii. Difference between Nabi and Rasool
iii. Characteristics of Prophets
iv. Status of Prophet and its requisites
v. Rational Proof of Prophethood of Muhammad (s.a.w)
vi. Attributes of Muhammad (s.a.w)
vii. Basis of relationship with the Prophet (s.a.w)
viii. Finality of Prophethood.
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c. Akhirat
1. Need and concept of Akhirah
2. Different concepts of Al-Akhirah in different religions
3. Qur’anic and rational arguments about Al-Akhirah
4. Concept of reward and punishment
5. Effects of belief in al-Akhirah on this life.
4. Pillars of Islam
Concept of Ibadah
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(Part 2)
Assignment/Projects:
Criterion:
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Inadequate introduction, Captivating introduction,
Low level critical thought and High level critical thought and
analysis analysis
Technology/Equipment/Models/Physical Support:
1. Multimedia
2. Overhead Projector
3. Audio Visual Lab
4. Movies
5. Audio Cassettes
6. CDs
Recommended Text Books::
1. Ahmed, Prof. Khurshid. (1986) Islami Nazaria Hayyat. Karachi: Karachi University Press
2. Ahmed, Prof. Khurshid, Islam, its meaning and message, Islamic book trust, Kuala Lumpur
3. Hamidullah, Muhammad, Introduction to Islam, Idara-e-Islamaiat, Lahore
4. Shaheed, Syed Qutsb. Islam the Misunderstanding Religion. Lahore: Islamic Publications.
5. Shaheed, Syed Qutsb. Islam aur Jadid Zahen ka Shubhat. Lahore: Islamic Publications
6. Hashmi, Dr. Ramiz Iqbal, (2003), The Science of Reciting the Qur’an, Rawalpindi, Pakcom
Printer, Peshawar Road.
7. Zakir, Naik, Concept of God in Different Religions
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8. Tauheed, Bilal Philips
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First Year Semester-2
Course Code RLT-607
Course Title Basic Anatomy-II
Credit Hours 3(2+01)
2. Genito-Urinary System
The structures and organs of the urinary system
The structure of the nephron
Organs of the Male Reproductive System
Organs of the Female Reproductive System
3. The Nervous System
Anatomical organization of Central Nervous System
Anatomical organization of Peripheral Nervous System
Anatomical organization of Autonomic Nervous system
The basic structure of a neuron, nerve, nerve tract, nucleus, and ganglion.
Cerebrospinal fluid and its circulation
4. Endocrine system
Endocrine glands
5. Anatomy of eye and ear
6. Receptors of touch, taste and smell
7. Practicals:
Study and understand anatomy of human body.
. Human models
.Video demonstration
8. Recommended Books:
Essentials of Anatomy and Physiology by Seely, Stephens, and Tate, 4th edition
Anatomy & Physiology by Rose & Wilson
General Anatomy by Ghulam Ahmad/ Laeeq Hussain
Anatomy by D.R Johnson & K.L Moore
Regional Anatomy by Snells.
Medical Laboratory Manual for Tropical Countries. Vol I, Second Edition by Monica
Cheesbrough
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Course Code RLT-608
Course Title Basic Physiology-II
Credit Hours 3(2+01)
1. The Nervous System
The functional areas of the cerebral cortex and their interactions.
Functions of the parts of the brainstem and cerebellum.
Functions of various cranial nerves.
Functions of the autonomic nervous system
The function of neurons.
Medical terms used to describe disorders of Nervous system.
o Meningitis, Encephalitis, Epilepsy, Hydrocephalus, Neuropathy, Palsy, Paralysis,
Poliomyelitis, Shingles, Stroke, Vertigo
2. The Endocrine System
Functions of the Endocrine System
Chemical Signals, receptors and hormones
The Endocrine Glands and their Hormones
Other Hormones
3. The Digestive System
Functions of each organ of the Digestive System
Movements and Secretions of the Digestive System
Physiology of Digestion, Absorption, and Transport
Terms used in gastrointestinal medicine and liver disorders.
Cholangitis, Cholecystitis, Cirrhosis of the liver, Gastrectomy, Haematemesis,
Haemoptysis, Hepatoma, Hiantus hernia, Melaena, Paralytic ileus, Pyloric stenosis, Sprue,
Ulcerative colitis, Vagotomy
4. Genito-Urinary System
Urine Production,
Regulation of Urine Concentration and Volume
Body Fluid Compartments
Regulation of Extracellular Fluid Composition
Physiology of Male Reproductive system—spermatogenesis and reproductive glands,
hormones and their regulations
Physiology of Female Reproductive system--- ovulation, hormones
Terms used to describe disorders of the Urinary system, Anuria, Cystitis, Diuretic,
Frequency, Glomerulonephritis, Nephrotic syndrome, Oliguria, Polyuria, Pyelitis,
Pyelonephritis, Pyuria, Renal calculus, Renal failure, Retention, Urinary tract infection UTI
, Uraemia
5. Immunity
Define immunity, Innate Immunity, Adaptive Immunity
Antigens and Antibodies
Primary and secondary responses to an antigen
Antibody-mediated immunity and cell-mediated immunity
Role of lymphocyte in immunity regulation
6. Recommended Books
Essentials of Anatomy and Physiology by Seelay, Stephens and Tate. 4th edition
Rose & Wilson Anatomy and Physiology
Human Physiology. Stuart Ira Fox 7th edition
Text book of Medical Physiology Guyton
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Essential of Medical Physiology Vol I & II by Mushtaq Ahmad
Medical Laboratory Manual for Tropical Countries. Vol I, Second Edition by Monica
Cheesbrough
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Balanced diet
Role of carbohydrates, fats and proteins, their dietary sources and uses
in the body
Quantitative and qualitative daily requirements of carbohydrates, fats,
proteins, vitamins and minerals
7. Practicals:
Determination of enzymes
Urine examination (Chemical examination)
CSF examination
Examination of Pleural, pericardial, synovial fluids
Recommended Books
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Course Code RLT-611
Course Title Communication skills (English –II)
Credit Hours 3(3+0)
Communication Skills
Aims
The course aims to equip students with communication skills suitable for their academic
purposes. It is further meant to develop among students the skills necessary for effective oral
and written communication in their profession and life in general.
Course Objectives
By the end of the course students would be able to:
Understand the dynamics and important dimensions of four skills: Reading, Writing
,Listening and Speaking
Communicate effectively both verbally and non-verbally
Relate to the interpersonal and organizational dynamics that affect effective
communication in organizations
Demonstrate understanding of the generic fundamentals of communication.
Course Contents
Reading: Fiction, poetry and Non-fiction
Writing a coherent paragraph
Effective Listening skills
Public speaking
Identification of Sentence errors
Seven C’s of Effective Communication
Phonetics and Phonology and its role in Communication
English Pronunciation
Developing Vocabulary
Effective Note Taking
Communicating effectively in Job Interviews
Oral Interaction: Dialogue and Conversation skills
Précis Writing
Critical Thinking Skills
Methodology
The course will be taught mainly through lectures, activities, drills, tutorials, group discussions,
simulations and student presentations.
Suggested Readings:
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1. The role of Muslim Students in the reconstruction of Islamic world by SayedAbul A’
alaMaududi
2. Bequest of Umme Hakimah for her Son. Al Huda Publications, Islamabad.
3. Why Islam is our only choice by Muhammad HanifShahid.
4. Ummah or Nation by Abdullah al- Ahsan.
5. Islamic Civilization foundational beliefs and principles by SayedAbul A’ alaMaududi.
1. Introduction to Computers
Usage and functionality of computers
Classification of Computers
Basic Components of Computers
2. Hard ware and soft ware
System Software
Application Software
Equipment’s/devices in Personal computer system
Input devices
Output devices
Storage devices
The processor
3. Microsoft Windows
Introduction to MS-Windows
Arranging, Moving and Resizing Windows.
Identifying the components of desktop.
Crating, Opening and Deleting items and folders.
Working with My Computer
Deleting and Resume Print Jobs.
Using Control Panel
Working with Accessories.
4. Microsoft Office
5. Microsoft Win Word
Microsoft Excel
Microsoft Power Point
E. mail
6. Practical.
Medical data entry
Patients reporting
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Second Year Semester -3
Course Code RLT-613
Course Title Behavioral Sciences
Credit Hours 2(2+0)
5. Memory
Define memory and describe its stages, types and methods to improving it
6. Thinking
Discuss problem solving and decision making strategies
7. Communication
Define communication. What are types, modes and factors affecting it.
Describe ways to recognize non-verbal cues. Characteristics of a good communicator
8. Learning
Define learning, Principles of learning, modern methods and styles of learning,
types of learners, Strategies to improve learning skills
9. Stress and Stressors
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Psychosocial issues in Emergency Departments, Intensive Care and Coronary Care Units,
Operating Theatres, Cancer wards, Transplant Units, Anaesthesia
Practical:
Communication Skills
Counseling,
Crisis Intervention
Conflict Resolution
Principles of effective communication, active listening, the art of questioning
Credit Hours: 2
Course Pre-requisite:
Pakistan Studies FA/F.Sc.
Course Duration: 16 weeks (Semester 2)
Course Description:
The course has been designed as a compulsory subject for the students studying for Bachelor’s degree,
general or professional. The course is of 3 credit hours carrying 100 marks. The teaching work is
comprised of three dimensions: Historical Perspective (20) %; Government and Politics (40) %; and
Contemporary Pakistan (40) %.
The course framework is issue-oriented. It has many dimensions, the historical and ideological back
ground of Pakistan the process of governance and national development as well as the issues arising in
the modern age and posing challenges to Pakistan. The course has been designed with a vision that
Pakistan Studies should open a window to future.
Course Goals:
1. The students will enable to acquire a sound knowledge and a balanced understanding of
the history and heritage of Pakistan, and the land, people and resources of the country.
2. To develop students’ analytical and critical capabilities and broaden their vision.
3. To prepare a good citizen for Pakistani community.
Course Objectives:
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1. General:
a. To develop the ideological vision of Pakistan.
b. To enable the students understand various dimensions and key concepts of Pakistan
economy in the light of past experience.
c. To comprehend the basis of Pakistani society and culture
d. To familiarize the students with the basic principles and objectives of the foreign policy
of Pakistan.
2. Specific
a. Knowledge:
To understand the historical and ideological background of Pakistan.
To understand the constitutional and political issues and problems of
Pakistan.
To examining the contemporary issues and problems based on the
patterns emerging out of the current situation of the world.
b. Skills:
After studying the course student will be skillful.
The students will be able to reduce the consequences of prejudice spreading all
over the country.
c. Attitude/Behavior:
The student will be able to promote unity and brotherhood among the Pakistani
society.
1. Interactive Lecture
2. Discussion
3. Demonstration
4. Field trip study
Course Contents:
1. Ideology of Pakistan
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a. Basic terminology
b. Two Nation Theory
c. Definition
d. Effects of ideology on Pakistani society
e. Aims and objectives of Pakistan
f. Two Nation Theory with reference to Sir Syed Ahmed Khan, Iqbal, Quaid-e-Azam, Syed
Maududi
2. People and land of Pakistan
a. Indus civilization
b. Muslim advent
3. Location and geo physical features
4. Government and politics in Pakistan
a. Political and constitutional phases
b. 1947-58
c. 1958-71
d. 1971-77
e. 1977-88
f. 1988-99
g. 1999-onward
5. Contemporary Pakistan
a. Economic institutions and issues
b. Society and social structure
c. Ethnicity
d. Foreign policy of Pakistan and challenges
e. Pakistan – US relations in the rapidly changing world
f. Pakistan – India complexities
g. Futuristic vision & outlook of Pakistan
Assignment/Projects:
Criterion:
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Poorly written paper Excellent written paper
Low level critical thought and High level critical thought and
analysis analysis
Technology/Equipment/Models/Physical Support:
1. Multimedia
2. Overhead Projector
3. White Board
4. Museum
Recommended Text Books::
Khan, Abdul Qadir. Pakistan Studies compulsory, Lahore: A H Publisher 22 Alfazal Market
Zafar, M.D Pakistan Studies, Lahore: Aziz Book Depot, Chowk Urdu Bazar
Burki, Shahid Javed. State & Society in Pakistan, The Macmillan Press Ltd. 1980
Saleem, Prof. Muhammad, Tareekh Nazria Pakistan, Islamic Publication, Lahore, 1995
1. Akbar, S. Zaidi. Issue in Pakistan’s Economy. Karachi: Oxford University Press, 2000.
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2. S.M. Burke and Lawrence Ziring. Pakistan’s Foreign policy: An Historical analysis. Karachi: Oxford
University Press, 1993.
3. Mehmood, Safdar. Pakistan Political Roots & Development. Lahore, 1994.
4. Ali, Ch. Muhammad, The Emergence of Pakistan, New York: Colombia University Press, 1967
5. Amin, Tahir. Ethno - National Movement in Pakistan, Islamabad: Institute of Policy Studies,
Islamabad.
6. Qureshi, I.H., The Struggle for Pakistan, Karachi: University of Karachi, 1969
7. Riaz Hussain, Prof., Pakistan Na Guzeer Tha, Karachi, University of Karachi, 1975
8. Zahid, Ansar. History & Culture of Sindh. Karachi: Royal Book Company, 1980.
9. Afzal, M. Rafique. Political Parties in Pakistan, Vol. I, II & III. Islamabad: National Institute of
Historical and cultural Research, 1998.
10. Sayeed, Khalid Bin. The Political System of Pakistan. Boston: Houghton Mifflin, 1967.
11. Aziz, K.K. Party, Politics in Pakistan, Islamabad: National Commission on Historical and Cultural
Research, 1976.
12. Muhammad Waseem, Pakistan Under Martial Law, Lahore: Vanguard, 1987.
13. Haq, Noor ul. Making of Pakistan: The Military Perspective. Islamabad: National Commission on
Historical and Cultural Research, 1993.
14. Maududi, Syed Abu’l A’la, Massla-e-Qommiat, Islamic Publications, Lahore, 1990
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4. Haemodynamic disorders
Define and classify the terms Edema, Haemorrhage, Thrombosis, Embolism, Infarction
&Hyperaemia with at least two examples of each
Define and classify Shock with causes of each
Describe the compensatory mechanisms involved in shock
Describe the possible consequences of thrombosis
Describe the difference between arterial and venous emboli
5. Neoplasia
Define the terms Dysplasia and Neoplasia with examples of each
Enlist the differences between benign and malignant neoplasms
Enlist the common etiological factors for Neoplasia
Define and discuss the different modes of metastasis
Practical:
1. Adaptive Response
Hyperplasia
Endometrial Hyperplasia
Gross, Microscopic
2. Acute Inflammation
Morphological patterns
3. Recognition of Ulcer , Abscess
4. Neoplasia – Gross examination of Cystadenoma ovary (Serous / Mucinous), Teratoma ovary,
Cystadenocarcinoma ovary, Lipoma, Fibroiduterus
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Lymphomas
Hodghkins lymphoma
Non Hodghkins lymphoma
Plasma cell disorders
Multiple Myeloma
Haemostasis
Normal Hemostasis
Hereditary Coagulation Disorders
- Hemophilia A
- Hemophilia B
Acquired Coagulation Disorders
- Vitamin K Deficiency
- Haemorrhagic disease of the newborn
- Liver Disease
- Disseminated intravascular Coagulation
Thrombosis
- Venous Thrombosis
- Arterial Thrombosis
Practical:
Students should be able to:
Prepare and use common anticoagulants
Operate fully Automated Haematology Analyzer
Preparation and staining of blood films
Reticulocyte staining and reticulocyte count
Bone marrow aspiration and trephine biopsy
Cytochemical stains
Haemoglobin studies
Screening tests for G6PD deficiency
Sickling test
Coagulation studies
Platelet function studies
Thrombophilia screening
Cellulose Acetate Membrane Electrophoresis
Quality control in routine haematology
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The normal bacterial flora of the body: symbionts, commensals, opportunists
Classification of bacteria
Gram positive cocci ( staphylococci, Streptococci)
Gram positive bacilli. (bacillus, clostridium, corynebacterium, Listeria)
Gram negative cocci. (meningococci, Gonococci)
Gram negative bacilli ( E. coli, salmonella, shigella, vibrioklebsiella, proteus,
campylobacter)
Spirochaetes actinomyces Israel, nocordiaasterioides)
Mycobacteria ( Mycobacterium tuberculosis, mycobacterium leprae)
Chlamydia, Rickettsia, Mycoplasma
Virus structure, viroids viron, prions
Viral classification on the basic of Capsid, envelope, Nucleic acids, Baltimore
Classification
Viral replication
Viral life cycle
Viral pathogenesis
Viral culture, Cell lines
Introduction to Mycology
Growth characteristics of fungi
Yeasts and molds
Fungal pathogenesis
Fungal culture media
Fungal staining
Introduction to Parasitology
Practicals:
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14. Stool examination
15. Blood film for haemoparasite
16. Semen examinations
17. Staining in mycology
18. Culture media used for fungus culture
19. Fungal culture
20. Methods of lab diagnosis of viruses
Lecture Details
Week: 1
Details: The Writing process: prewriting, Writing and Rewriting, Need and significance of Business Communication,
Main forms of Business Communication , Difference between business and other forms of wirings, prominent
features of business communications,
Week: 2
Details: Essentials parts of a business letter, forms and styles of modern Business letter, Comparing good and bad
examples of business letters, Widely Known formats of letter: Indented Form, Semi Block Form, Block Form, Full-
Block Form
Activities and Tasks: To rewrite the letters after making required grammatical correction
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Week: 3
Details: Kinds of Business Letter: Inquiry Letters, Order Letters, Claim/Complaint Letter, Apology Letters, Official
Invitations, Thanks Letters, Resignation Letters, Letters of Recommendation, Identifying various types of
correspondence: very informal, very formal, neutral, informal, formal, very formal
Activities and Tasks: To write at least three letters of each situation mentioned above
Week: 4
Details: The main purpose(s) of a Cover Letter, Structure and Format of a Cover Letter, Reviewing the best
specimens of Cover Letters,
Activities and Tasks: To write Cover Letter for different situation assigned by the teacher
Week: 5
Topic: CV Writing
Details: The main purpose of a CV, Key Characteristics of an Effective CV/Resume, Various Formats of CV, Typical
Errors in CV Writing, Comparing and Reviewing good and bad examples of CVs/Resumes
Week: 6
Details: What and Why of an Executive Summary? Steps/Processes involved in writing an Executive Summary,
language and style, What not to include in the summary?, Strategies to make the summary clearer and concise,
Editing and revising, checklist of an Executive Summary, Analysis of the best examples of Executive Summaries
Week: 7
Details: What is a report and how is it different from other forms of writing? Stages/Steps in writing a report,
Terms of reference, planning your report, collecting information, organizing information, structuring a report, style
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of writing, layout, presentation, redrafting and revising, Checklist
Week: 8
Details: What is an MoU and why is it significant? Possible situations to write an MoU, Steps involved in writing an
MoU, Structure, layout and language of an MoU, Best samples of MoUs and their analysis
Activities and Tasks: To analyze the best samples of MoUs signed by govt/orgs
Week: 9
Week: 10
Details: The purpose of Minutes, Steps involved in writing Minutes, Language and style of Minutes Different
Formats for writing minutes ,What should be included and What not to include in the Minutes, Minutes writing
process, Analysis of various specimens of meeting minutes
Assignment: To select a specimen of a meeting minute and make a brief power point presentation
Week: 11
Activities and Tasks: What is a proposal? The purpose and significance of writing a proposal, Types of Proposals,
Essentials elements and structure of a proposal, language, style and format, presentation of data, checklist of a
proposal
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Week: 12
Details: What is a Research paper? Structure and Format of a Research paper, In-Text citation, references,
language of a research paper, Difference between Abstract and Introduction, language and style, Citation styles
Quiz
Week: 13
Details: Rules and Strategies to structure a presentation, Mechanics used to make a good presentation,
Week: 14
Topic: Presentations
Details: The students will make presentations in groups on the assigned theme
Week: 15
Topic: Presentations
Details: The students will make presentations in groups on the assigned theme
Week: 16
Recommended Books
Technical Communication: Process and Product by Sharon Gerson and Steven Gerson
Effective Business Communication and Report Writing by Prof. Sh. Atta ur Rahman
Modern Business Correspondence by L Gartside
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Second year Semester 4
Course Code RLT-619
Course Title Clinical Pathology
Credit Hours 2(01+01)
Collection and Transport of Specimens, Examination of specimens
Type of specimens
Time of collection
Collection techniques
Labeling of specimens and the sending of a request form
Specimens containing dangerous pathogens
Preservatives and transport media for microbiological specimens
Transport of microbiological specimens collected in a hospital
The examination of urine
Physical examination
Volume
Colour
Appearance
pH
Specific Gravity
Chemical examination
Proteins
Methods of Estimation:
. Turbidimetric Method (Heat Method, Acid Precipition, Sulfosalicylic Acid Test
(Kingsbury and Clark)
. Colorimetric
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Bence jones proteins (BJP)
Heat Precipitation Test
Esbach's Test
Pyrogallol Red-Dye Test
Glucose and reducing sugars
Monosaccharides
Disaccharides
Methods of estimation
Benedict's Test
Enzymatic test
Bile pigments (bilirubin)
Foam Test
Dye Dilution Test
Fouchet's Test
Bile salts
Hay’s Test
Blood
Reduced Phenolphthalein Test
Pyramidone Ring Test
Benzidine Test
Commercial Dipstick Test
Nitrite
Commercial dipstick test
Ketone bodies
The Rothera Tube Test
Gerhardt's Test (Ferric Chloride Test)
Urobilinogen
Spectroscopic Examinations
Ehrlich's Benzaldehyde Test
Phenylketonuria
Ferric Chloride Test
Porphobilinogen
Watson-Schwartz Test
Porphyrin
Chloride
Fontana's Test
Microscopic examination of urine
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Light Microscopy
. Leucocytes
. Erythrocytes
. Casts
. Epithelial Cells
. Amorphous Deposits
. Crystals
. Miscellaneous
. Dark-ground illumination
Microscopic test for fat
This test is based on staining of fat with Sudan
Automated instrumentation for routine urine examination
Color
Odor
Consistency
Parasites.
pH
Microscopic examination
Direct wet preparation
Concentration techniques
o Sodium chloride floatation technique
o Zinc sulphate floatation procedure
Protozoa
Entamoeba histolytica
Unstained Trophozoites
Stained Trophozoites:
Unstained Cysts
Stained Cysts
Giardia lamblia
Helminths
Enterobius vermicularis
Strongyloides stercoralis
Trichuris trichiura
Ancylostoma deudenale (Hookworm)
Schistosoma haematobium
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Schistosoma japonicum
Schistosoma mansoni.
Test for blood in faeces
Blood in faeces can be detected by:
Benzidine Test
The Orthotoludine Test
The examination of cerebrospinal fluid (CSF)
Normal CSF composition
Proteins
Glucose
Chlorides
Sample collection and storage
Routine examination
Appearance
Cell Counts
Microscopic Examinations
The estimation of proteins
Mestrezat's Diaphenometric Procedure
Sulfosalicylic Acid Test
Biuret Method
Dye-Binding Method
Pyrogallol Red Method:
The estimation of globulins
Pandy's Test
Nonne-Apelt Reaction
The estimation of glucose
The estimation of chloride
CSF culture
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Pleural fluid
Pericardial fluid
Synovial fluid
Hydrocoele fluid
Aspirates from cysts, etc.
Peritoneal fluid
Criteria for diagnosing blunt and penetrating trauma by peritoneal lavage fluid analysis
Appearance of peritoneal fluid and associated diseases
Microscopy of peritoneal fluid
Chemical analysis peritoneal fluid
Protein
Glucose
Enzymes
Tumour Markers
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Course Code RLT-620
Course Title Basic histopathology
Credit Hours 4(02+02)
1. Tissue fixation
2. Gross cutting (Observation. To handle common surgical pathology specimens including
importance of slicing large specimens. Salient features to be described as per
recommended guidelines.)
3. Tissue processing
4. Special precaution for tissue to be sent for (electron microscopy, special
biopsies, immuno-fluorescence etc)
5. Decalcification
6. Basic Molecular Biology Techniques & FISH testing for Her-2-neu/solid tumors
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a. Preparation of blood smear
b. Staining of blood smear
c. Types of Romanowsky staining (Leishman’s , Giemsa’s, Field’s stain)
d. Blood cells morphology (Neutrophils, Lymphocytes, Monocytes , Eosinophils, Basophils)
Coagulation
Mechanism of coagulation (role of blood vessels, platelets and clotting factors)
Estimation of coagulation (BT, CT, PT, APTT, TT,FDP’S, D-Dimers)
Equipment required for Coagulation studies
Manual
Automated
Techniques for the diagnosis of the following
anaemias
i. Iron deficiency
ii. Megaloblastic Anemia
iii. Hemolytic Anemia
iv. Sideroblastic Anemia
v. Thalassemia
vi. Sickle cell Anemia
vii. Aplastic Anemia
Blood coagulation disorders
The techniques, principle, procedure and interpretation of the following tests:
Bleeding Time
Clotting Time
Prothrombin Time
Activated Partial Thromboplastin Time
Thrombin Time
Correction studies
Fibrin Degradation Products
D-dimers
Practical
Study of peripheral blood smear for WBCs , RBCs and platelets
Automation in hematology,
Hematology cell counter, principle, usage and precautions for daily maintenance
Start up and shut down procedures
Record of maintenance
Coagulation analyzers
Hb estimation
ESR
RBC Count
WBC Count
Platelet Count
Eosinophil Count
Coagulation assays BT, CT, PT, APTT, TT
Bone marrow biopsy: Preparation of instrument tray and to assist Hematologist in performing
Bone marrow biopsy
Hb electrophoresis
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Course Code RLT-622
Course Title Clinical Chemistry Techniques/Instruments
Credit Hours 4(02+02)
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Flame Photometer
Ion selective analyzer
ABG analyzer
Plasma and urine Osmolality
Chemistry auto analyzer
---------------------------
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Checking of efficacy of autoclave
13. Sterilization and disinfection
Practicals:
1. Specimen collections, labeling and handlings
2. Microbiology laboratory hazards and precautions
3. Urine examination and culture
4. Stool examination and culture
5. Blood film for haemoparasite and culture
6. Semen examinations
7. Staining in mycology
8. Culture media used for fungus
9. Fungal culture
10. Methods of lab diagnosis of viral diseases
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Third year semester 5
Course Code RLT-624
Course Title Basic Medical Immunology and Serology
Credit Hours 3(02+01)
1. General immunology
A. Innate and adaptive immunity
B. Antigen and antigenecity
C. Antibodies and immunoglobulin
D. B-cell maturation, activation, differentiation and memory
E. Basic structure of antibodies
F. Antibody-mediated effector functions
G. Antibody classes and biological functions
H. B cell responses
I. Primary and secondary immune response
J. Cell mediated and humoral immunity
K. Immune system
L. Hypersensitivity
M. Autoimmunity
N. Immune mediated diseases
O. Immunization
P. Immune deficiency
2. Components of the nonspecific immune system
A. Mechanical barriers
B. Chemical and biochemical barriers
C. Cellular components (NK cells, Polymorphonuclear phagocytes, marcophages)
D. Complement system and pathways
E. Molecular and cellular mechanisms involved in inflammation
3. Immunological Techniques
A. General considerations
B. Agglutination reactions
C. Precipitation reactions
D. Immunodiffusion techniques
E. Double diffusion
F. Single redial Immunodiffusion
G. Immunoelectrophoresis
H. Complement fixation test
I. Immunoflourescence
a. Direct
b. Indirect
J. Enzyme Immunoassay (EIA)
4. Immunochemical Techniques
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A.
Quantitation of IgG, IgA, IgM, IgD, and IgE in serum and other body fluids
B.
Immunoelectrophoretic analysis of serum immunoglobulin abnormalities
C.
Detection /quantitation of Bence-Jones protein in the urine
D.
Cryoglobulin determination and analysis
E.
Test for circulation immune complexes by immunochemical methods
F.
Immunochemical and electrophorphoretic analysis of CSF
G.
Measurement of overall complement function
H.
Total haemolytic and alternative pathway titrations of complement components (especially
C3, C4, factor Band C1esterase inhibitor)
I. Electrophoretic examination for altered complement components
J. Other serum protein determinations including acute phase proteins (CRP etc.),
Carcinoembryonic antigen, a fetoprotein and protein clearance rations
K. Pregnancy tests on urine
5. Immunohistological Test
A. Detection of antigens, antibodies, immunoglobulins and complement components
deposited in pathological lesions, particularly in the kidney and skin.
B. Characterization of plasma cells and lymphocyte types in relevant tissue biopsies, using
immunofluorescent and enzyme-labeled techniques.
Practical
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Course Code RLT-625
Course Title Basic Medical virology & Mycology
Credit Hours 3(02+01)
1. Classification of Viruses
2. Basic knowledge of the of common viral infections
3. Lab test for viral diagnosis
4. Laboratory safety and house keeping
VIROLOGY AND MYCOLOGY
Introduction
Features, structures and classification of viruses
Virus culture
Viral pathogenesis
Hepatitis viruses (A, B, C,D & E)
HIV
HSV, VZV, CMV, EBV, Small pox and Adeno virus
Adenovirus, HPV
Denguevirus, Yellow fever virus equine encephalitis virus
Tumor virus
Picrona virus _Polio
Infulenza, measle, mumps, rubella, rabies
Polio virus, rhinovirus, rota virus
Bacteriophages
Specimen collection & isolation of viruses
Basic mycology
Introduction
Classification
Morphology and structure of fungi
Nutrition and cultivation of fungus
Cutaneous & Sub cutaneous and systemic Mycosis
Lab diagnosis of fungal infections
Opportunistic fungal infections
Identification of dermatophytes
Practicals:
Staining in mycology
Culture Media used for Fungus
Fungal Culture
Methods of lab diagnosis
Methods of lab diagnosis for bacterial and viral diseases
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Course Code RLT-626
Course Title Basic Transfusion Medicine
Credit Hours 4(02+02)
1. Blood Banking
a. Introduction
b. Requirements for establishment of blood bank
c. Human blood group system (ABO, Rh, and other important blood group systems)
d. Blood grouping: Forward and reverse grouping
2. Coombs test
3. Blood Transfusion
Principal and practice of blood Transfusion
Blood Transfusion service at district level
Guidelines for the use of blood, appropriate use of blood, quality assurance
Autologous blood transfusion practices
Objectives of quality assurance in blood transfusion services
Standard operating procedure for usage, donation and storage of blood
Screening of donor and compatibility testing
Safety and procurement of blood components.
4. Blood Donation
a. Introduction
b. Voluntary blood donation
c. Criteria for selection of donor
d. Medical history and personal details
e. Self-exclusion
f. Basic lab tests before donating blood
g. Screening for TTI
5. Blood Collection:
Blood Bags
b. Anticoagulants (CPD-A1, CPD-A2)
c. Taking and giving sets in blood transfusion
d. Blood donation procedure
e. Post donation donor care
f. Reaction during and after donation
6. Testing donor blood
Screening donors blood for infectious agents – HIV, HCV,HBV, Treponema pallidum, malarial parasite
7. Blood donor records
Blood donor record book
Recording Results
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8. Storage and Transport
a. Storage of blood (RCC) / Components
b. Changes in blood after storage
c. Refrigerated centrifuge
d. Lay out of a blood bank refrigerator
e. Transportation of Blood components
9. Maintenance of blood bank records
a. Blood bank temperature sheet
b. Blood bank stock sheet
c. Blood transfusion request form
10. Compatibility testing
a. Purpose
b. Single tube compatibility techniques using AHG reagent
Practicals
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Course Code RLT-627
Course Title Biostatistics and research methodology
Credit Hours 2(0+2)
Course content
1. Introduction to statistics
a. Statistical data condensation of data
b. Presentation of data by graphs
c. Health related data
d. Rates and their relative importance
e. Presentation of quantitative data
2. Sampling
a. Concept of sampling
b. Type and methods of drawing ideal sample
c. Sampling distribution of sample mean
d. Error of sampling
e. Standard error
f. Chi square
g. T-test and their uses in health
2. Central Tendency
a. Concepts of central tendency
b. Mean, mode and median and their value in health
c. Percentiles
d. Measure of dispersion
e. Coefficient of variation and skewness
f. Normal distribution
g. Range, Standard deviation and relative deviation
3. Hypothesis
a. Concept of Hypothesis testing
b. Null and alternative Hypothesis
c. Two type of errors
d. Acceptance and rejection regions
e. Two sided and one sided tests
f. General steps in Hypothesis testing
g. Test about means, confidence interval for mean, meaning significance in statistical
procedures and methods of inferential statistics
4. Regression and correlation
a. Scatter diagram
b. Straight line regression model
c. Method of least squares
d. Sample correlation coefficient
e. Inference about regression coefficient and correlation coefficient
5. Introduction to Research
a. The question of legitimate knowledge
b. Knowledge and decision making, the scientific method
c. Quantitative vs qualitative research
d. Application of scientific method
e. Positivistic vs naturalistic paradigm
6. Classification of research
a. Basic vs applied research
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b. Evaluation research
c. Research and development (R& D)
d. Action research
7. Selection and formulation of a problem
a. From generic to a specific problem, program statement
b. Getting an access to primary and secondary resources
c. Note taking and information to management
d. Review of related literature
e. Questions and/or hypothesis of the study
8. Development of a research plan
a. The ethical, legal and professional obligations
b. The rational of the study
c. The research plan
d. Evaluation of a research plan
9. Selection of sample
a. Sample and population
b. Basic considerations in sampling
c. Random sampling, stratified random sampling cluster sampling
d. Systematic sampling determination of sample size and elimination of sampling bias
10. Instrumentation and data collection
11. Data analysis and interpretation
12. Preparation of research report
13. Introduction to research (in sample term and a scientific term), concept of research, why do
need research, advantage of research, identification of research need and its qualities,
component of research, ethical and legal aspect of research and objective of research
(definition, purpose, structure)
14. Selection of topic
A. The criteria points of prioritization of topic
a. Relevance
b. Avoidance of duplication
c. Physibility
d. Political acceptability
e. Applicability
f. Cost efficiencies
g. Ethical consideration and scales for rating research topic mentioned above
B. Work plan
a. It’s content
b. How to develop a work plan
c. What factor should be kept in mind while preparing research work plan
C. Budget required for research work
a. Why it is needed?
b. What should be taken in to consideration?
c. Literature searching
d. Statistical help
e. Material
f. Type of manuscript
g. Printing of manuscript for submission and postage
15. Concepts of measurement
a. Principals and reliability of measurement
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b. Errors and sources of measurement,
c. Type of measurement
d. Measure of disease frequency and screening (introduction, validity and screening test)
16. Designing clinical research
a. Studies design (introduction, selection of design)
b. Research questionnaire
c. Validity and reliability of research finding, confounding factors
d. Strategies to deal with threats to validity
17. Hypothesis test
18. Sampling
a. Probability and non-probability and their sampling
b. Advantages and disadvantages of probability and non-probability sampling, bias in
sampling
19. Research protocol
20. Data collection
a. Purpose and techniques
b. Why do we collect data?
c. Data collection procedure
d. Step and data collection survey questionnaire, starting questionnaire.
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c. Duodenale (Hook worm)
B. Tissue Nematodes.
C. W. Bancrofti – life cycle, morphology, diseases & lab diagnosis
9. Phylum Platyhelminths
A. Cestodes- T. solium, T.saginata & E. granulosus. (In brief)
B. Trematodes – S. haematobium & F. hepatica (In brief)
10. Role of Arthropods in the transmission of diseases.
11. Mosquito: Morphology and Bionomics of Anophales, Culex, Aedes and Mansaonia.
12. Mosquito – Borne diseases and their control
13. Phlebotomus: Morphology, life – history and control
14. House fly- Morphology, life cycle, disease relationship and control
15. Tse-Tse fly (glossina) morphology, life-cycle and public health importance.
16. Fleas: Morphology, life cycle, disease transmitted and control
17. Louse: Morphology, life cycle, disease transmitted and control
18. Bed bug: Life cycle and control
19. Ticks: Morphology, life cycle, disease transmitted and control
20. Sarcoptisscabiei: Morphology, life cycle, public health importance and control
21. Cyclops and Public Health importance.
Practical
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Course Code RLT-629
Course Title Histopathology Instruments / Techniques
Credit Hours 3(02+01)
1. Automation in Histopathology
2. Specimens for Histopathology
Basic Techniques
Biopsy Specimen collection including cytology specimens
Tissue fixation (Rationale, Types & Techniques
Gross cutting (Observation. To handle common surgical pathology
Specimens including importance of slicing large specimens.
Salient features to be described as per recommended guidelines).
Tissue processing (Steps & Rationale)
Special precaution for tissue to be sent for (electron microscopy, special biopsies, immune
fluorescence etc)
Decalcification (Methods
Basic Molecular Biology Techniques & FISH testing for Her-2- neu /common solid tumor testing
Specimen, slides and block storage protocols
Staining
Routine H & E staining
Special stains
Immuno histochemical staining, requirements and principles
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Third Year Semester -6
1. Glucose
2. Cholesterol
3. Triglycerides
4. HDl and LDL
5. Urea
6. Creatinine
7. LFTs
8. Albumin
9. Calcium
10. Phosphate
11. Uric acid
12. Iron Metabolism
13. Muscle Enzymes
14. Cardiac markers
15. Gastric, Pancreatic and Intestinal Function Tests
16. Electrolytes
17. Blood gases
18. Pathophysiology of Endocrine disorders and lab diagnosis of following:
Anterior and Posterior pituitary hormones, hypothalamic hormones. Pituitary Disorders
Thyroid Disorders
Adrenal Glands. Hormones of the adrenal medulla and cortex. Hypo and Hyper secretion.
Cushing’s syndrome. Addison’s disease.
Parathyroid effects on Ca and Phosphorus
Insulin effects on Glucose metabolism, secretion, structure. Hyperglycemia, Diabetes Mellitus.
Antagonistic hormones 50
Male and Female sex hormones. (Testicular/ Ovarian/ Placental Hormones).Synthesis, secretion,
actions, metabolism. A little information about Amenorrhea, Oligomenorrheoa, Hirsutism,
Virilism, Spermatogenesis, Ovulation, Climacteric.
Tumour Markers List of commonly used tumor markers in different diseases and their clinical
utility.
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Course Code RLT-631
Course Title Haematology Lab Rotation
Credit Hours 4(01+03)
Analysis and interpretation of laboratory data in following analytes:
1. Estimation of Haemoglobin (Hb)
2. Hemoglobin disorders
3. Hemoglobin electrophoresis
4. Total Red Blood Cell Count (TRBC)
5. Packed Cell Volume (PCV) or Haematocrit (Hct)
6. Calculation of Red Cell Indices (Absolute Values)
7. Total Leucocyte Count (TLC)
8. Platelet Count
9. Reticulocyte Count
10. Erythrocytes Sedimentation Rate (ESR)
11. Preparation and Staining Of Blood Films
12. Study of Peripheral blood smear
a. Normal
b. In different types of anemias , WBC and Platelets disorder
13. Differential Leucocyte Count (DLC)
14. Bleeding Time (BT)
15. Whole Blood Clotting Time
16. Prothrombin time (PT)
17. Activated Partial Thromboplastin Time (APTT)
18. Thrombin Time (TT)
19. Assisting bone marrow aspiration and trephine biopsy
20. D-Dimers
21. Sickling test
22. Osmotic fragility test
23. Ham’s test
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The following stained techniques will be learnt & practiced
Gram staining
Ziehl-Neelsen staining for Mycobacterium tuberculosis
ZN staining for M. leprae
Auramine (fluorochrame) staining
Methylene blue
Wayson’s
Albert
Giemsa
HISS
Acridine orange (Fluorochrome)
Type of staining basic stains and Acidic stains
Mordants
Decolorization
Technique of staining smears
Quality control
Smear making
Aseptic technique
Making a wire loop
Inoculation of culture media in petri dishes, slops, as stabs and fluid media
Incubation of cultures
Temperature of incubation
Humidity
Gaseous atmosphere
Culturing of anaerobes
Anaerobic jars using hydrogen from a cylinders
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Gas generating kits method
Use of copper coated steel wool to remove oxygen
Use of dithionite in petri dishes
Use of reducing agents in culture media
Culturing in Carbon-dioxide
Candle jar
Gas generating kits
Control of culture media
Control of stains & regents
Arylsulphatase
Bile Solubility
Catalase
Coagulase
DNAse
Hydrogen sulphide (H2S)
Indole
Litmus milk decolonization
Nitrate reduction
Oxidase
Oxidation fermentation
Phenylalanine deaminase
Tween hydrolysis
Urease
Voges-Proskaur
Methyl Red
Sputum
Throat / mouth specimen s
Ear- discharges
Eye specimens
Skin and ulcer specimens
Pus from wound, absences, burns & sinuses
Urogenital specimens
Faeces (stools)
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Urine
CSF
Blood & bone marrow
Specimen for viral studies
Fungal specimens
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10. Immunohisto chemical staining, Principles (Technique) and common
applications
11. Basic museum techniques Any specimens for museum are handled by
following steps:
1. Reception
2. Preparation
3. Fixation
4. Restoration
5. Preservation
6. Presentation
12. Mounting of specimen
13. Museum jars and boxes
14. Attaching specimen to center plate
15. Special methods macerated specimen of bones
16. Plastination a technique to preserve whole bodies or body parts water and fat are replaced by
certain plastics specimens can be touched, do not smell or decay retain most properties of
original sample
17. Demostration of bronchial tree by plastics
18. Dlastination of abnormal fetuses transparent specimen
Course Description:
Credit Hours: 2
Course Pre-requisite:
Course Description:
Introduction to Qur’an, Arabic Grammar, Fundamental Principles and teachings of Islam through study
of selected verses from the Holy Qur’an
This course highlights the different aspects of life and will help in enlightening the minds of the students.
It will give them a chance to have an authentic knowledge of the current issues that they confront in
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their day-to-day life. Then they will also be able to apply this knowledge of Qur’an in their Practical life.
The students will be given an insight of Islamic laws, regulations particularly in perspective of the
modern world and its prevailing morality. This knowledge will not only give them awareness of the
routine matters but also offer them an opportunity to learn the basics of the Arabic Grammar. This will
help them in the better understanding of Qur’an.
Course Goals:
1. To impart an understanding of the basic principles and teachings of Islam through study of
selected verses of Qur’an.
2. To develop ethical, knowledgeable and practical professional.
3. To prepare MUTTAQEE and good Muslim professional.
Course Objectives
1. General:
a. To learn the true concept of Allah, values and needs of the society.
b. To motivate themselves for determination of the character.
c. To develop an Islamic professional who can work for Islamic welfare state.
d. To prepare the students for adaptation of Islamic way of life in modern age.
2. Specific
a. Knowledge:
i. To offer an introduction to the Holy Qur’an
ii. To inculcate the basic concept of the Qur’anic teaching.
iii. To know about the etiquettes of DUA.
iv. To understand the qualities of believers.
b. Skills:
i. To recite the Holy Qur’an with correct pronunciation and annotation.
ii. To make them capable for understanding and translate the Holy Qur’an with the
help of Arabic Grammar.
iii. To communicate effectively (written, oral and inter-personal).
iv. To solve the problems in the light of teachings of the Holy Qur’an.
c. Attitude/Behaviour:
i. To adopt good manners and avoid social evils.
ii. To develop the characteristics of MUTTAQEEN.
iii. To develop honest and responsible personality.
Educational Methodology to be used:
1. Interactive Lecture
2. Translation
3. Discussion
4. Demonstration
5. Micro Teaching
Course Contents: (Part 1)
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2 Introduction to the Qur’an
a. Makki Surahs
b. Madani Surahs
c. Revelation of the Qur’an
d. Miracle of the Qur’an
e. Manners of the Recitation
f. Obligations toward the Qur’an
g. Compilation of the Qur’an
h. Uloom-ul-Qur’an (the science of the Quran)
3. Arabic Grammar
Noun and its different types
a. By Capacity
b. By Gender
c. By Numbers
4. Pronouns
a. Attached Pronoun with Tables
b. Separated Pronoun with Tables
5. Verb and its Types
a. Past
b. Imperfect
c. Imperative
6. Letter and its Types
a. Preposition
b. Haroof-e-Jarah selective study of the Quran
7. Surah Al-Fatihah
a. Introduction
b. Different Names
c. Importance in the light of Hadith
d. Preface of the Quran
e. Comprehensive combination of praise of Allah and Dua
8. Surah Al-Baqarah (R 1)
a. Introduction
b. Characteristics of Muttaqeen
9 Surah An-Nisa (R 2 - 4)
a. Introduction
b. Inheritance
c. Fornication
d. Forbidden Relation regarding Nikkah
(Part 2)
10 Surah Al-Furqan (R 6)
a. Characteristics of believers
b. Tauba
c. False Witness
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11 Surah Luqman (R 2)
a. Shukker
b. Shirk
c. Raza’at
d. Rights of parents
e. Bad Manners (Raza’ail-e-Akhlaq)
12 Surah Al-Hujurat
a. Etiquettes about the honor of Allah and his Rasool
b. Confirmation of any news
c. Razail-e-Akhlaq
d. Concept of TAQWA
13 Surah Al-Noor (R 1)
a. Punishment of Adultery
b. Punishment for false witness (Had-e-Qazaf)
c. Accusation (Lian)
14 Surah Al-Ahzab (R 4 & 8)
a. Status of Ummhat Ulmomenin
b. Restrictions for woman in Islam
c. Prohibition of co-society
Assignment/Projects:
Criterion:
Low level critical thought and High level critical thought and
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analysis analysis
Technology/Equipment/Models/Physical Support:
1. Multimedia
2. Overhead Projector
3. White Board
4. Movies
5. Audio Cassettes
6. Audio Visual Lab
7. CDs
8. Websites
Recommended Text Books
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Fourth Year semester -7
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Course Code RLT 636
Course Title Epidemiology and Community Medicine
Credit Hours 2(1+1)
Introduction to epidemiology
Determinants
A. Primary
B. Secondary
Clinical epidemiology
Occupational epidemiology
Importance of epidemiology
Definitions of common terms related to epidemiology?
Health Indication
Concept of Health and Disease
Definition of health (Dimensions, physical, mental, social and spiritual).
Spectrum of health, Determinants of health.
Responsibility for health
Indicators of health
Concept of disease
Concept of causation (all theories including ecological triad, (agent, host & environmental
factors)
Spectrum of disease
Iceberg phenomenon
Natural history of disease
Levels of prevention
Disease elimination and eradication
Disease surveillance
Introduction to Public Health and Health Systems in Pakistan
i. Background and concepts: Definitions and concepts in Public health
Development of public Health in Pakistan
Economics and Health
Health policy and planning in Pakistan. “Health for all” background, concepts and progress.
“Primary Health care”: Concepts and progress.
The national disease control programs; polices, strategies and operations.
A. Health system in Pakistan: The role of Federal and Provincial Governments in
Health care.
The district health system, in the context of devolution
The physician as a manager: Functions of manager, management of material, human and
financial resources.
Leadership and motivation
Partners in health
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Course Code RLT 637
Course Title Medical Sociology
Credit Hours 2(0+02)
Course content
1. General
Definition and scope of sociology, sociology is a science, Islamic sociology
2. Medical Sociology
Introduction contribution of sociology to medicine
3. Health and disease
Social definition of illness, social and emotional component of illness
4. Patient and paramedic
Paramedics view of disease and patient, psychology of patient / paramedic
relationship
5. Mental illness sociological perspective
Definitions, social implication of mental illness
6. Rehabilitation
Definition, physical, mental handicape
Method in rehabilitation: Guidness counseling and vocational training
7. Social Disorganization
The concept and factor of social organization, family, group and community
disorganization
8. Problem of community
Problem of crime, method of treatment and preventive measure
9. Educational problems
Deterioration of education standard in school, college and university
10. Health problems
Illness behavior, delivery and utilization of health services
11. Introduction to applied sociology
Definition of applied sociology, nature and causative analysis of social problem and
the role of sociologist in solving social problems
12. Application of social research in social problems
Social survey and social research, nature purpose and function
Credit Hours: 2
Course Pre-requisite:
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Islamic Ethical Principles and Contemporary issues (Islamic Studies)
Basic Teachings of the Qur’an (Revealed Sciences - I)
Course Duration & Sequence: 32 weeks (Semester 7 & 8)
Course Description:
This course will introduce the Seerah of Holy Prophet Muhammad (SAW) and His Sayings, the status of
the Hadith and Seerah in the Islamic faith and Shariah. It is divided into two parts. First part comprises
the introduction of hadith, its authoritative position, compilation, terminologies, types and its sources
while the second part consists of introduction to the Seerah and different aspects of Prophet’s life and
status of Prophet in the DEEN.
This course will be university required course and partially fulfill the requirement of HEC Islamic studies
undergraduate compulsory course.
Course Goals:
1. General:
a. To familiarize the students with the status and authoritative position of Prophet
Muhammad (SAW).
b. To build up the information in Hadith and Seerah perspective.
c. To understand the preservation and transference of Hadith.
d. To learn the terminologies of Hadith.
2. Specific:
a. Knowledge:
i. To understand the science of Hadith.
ii. To describe the methods for preservation and transference adopted by Muslim
scholars.
iii. To have sufficient knowledge on the life of the Holy Prophet (SAW).
iv. To inculcate the true spirit of Islam through selected textual study.
b. Skills:
i. To develop the reading skill for Arabic text of Hadith.
ii. To create the positive and critical thinking.
iii. To enable the students to seek and utilize the basic sources of Shariah.
c. Attitude/Behaviour:
i. To develop a personality under the influence of moral teachings.
ii. To follow the exemplary pattern of Holy Prophet’s (SAW) seerah in every aspect
of life.
Educational Methodology to be used:
1. Interactive Lecture
2. Translation
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3. Discussion
4. Demonstration
Course Contents: (Part 1)
(Part 2)
Criterion:
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c. Free of spelling and grammatical errors
d. Appropriate length
e. Appropriate presentation (font type and size, margin, stapled)
Low level critical thought and High level critical thought and
analysis analysis
Technology/Equipment/Models/Physical Support:
1. Multimedia
2. Overhead Projector
3. White Board
4. Movies
5. Audio Cassettes
6. CDs
7. Websites
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Recommended Text Books::
1. Molecular science
b. Nucleic acid chemistry
Sugars
Bases
Chemical structure
Associated proteins
Mutations
c. Basic Molecular Theory
Replication
Transcription
Exons, introns, and splicing
Translation
Chromosome structure
Extra chromosomal structure (e.g., phage,
plasmid, mitochondrial)
Protein structure
d. Biochemical Reagents
Polymerase enzymes
DNA
RNA
Endo and exonuclease enzymes
Reverse transcriptase
DNA ligase
Assay development and design
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e. Genetics
Introduction to Human genetics
Chromosomal structure and function
Chromosomal anomalies and human disorders
Organization and expression of human genome
Cancer genetics
Functional genomics, Proteomics and Bioinformatics
Over view of microbial genetics
f. MOLECULAR TECHNIQUES
b. Nucleic Acid Isolation
Automated methods
Manual methods
c. Separation and Detection
Electrophoresis
Gel (including agarose and acrylamide)
Capillary
2. Blotting and probing procedures (including washing and stringency)
3. Probe hybridization
4. Nucleic acid purification
5. Probe structure (e.g., TaqMan®, FRET, simple, beacon, Scorpions®)
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6. Array technology (e.g., bead, microarray)
Practical
1. Mendelian Genetics
2. Human pedigree analysis
3. Analysis of various stages of cell division
4. Karyotyping
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Forth year Semester -8
Course Code UR-550
Course Title Life and Living
(Professional Ethics in Medical Science)
Credit Hours 2(02+0)
Course Description:
Credit Hours: 2
Course Pre-requisite:
Course Description:
This course highlights the importance of moral conduct in human life. First part of the course will
familiarize the students with ethical viewpoint of Islam. The second part will develop student’s
understanding about historical, social, ethical and professional issues related to the discipline of Medical
Science. It identifies the sources of information about professionalism and ethics. Students evaluate at
access ethical and professional in medical case studies. This course introduces the students to ethical,
moral issues and their responsibilities.
Course Goals:
1. General:
a. To build up personal and professional qualities in the light of Islamic ethics.
b. To acquire the knowledge of basic global ethical principles enshrined in the Islamic
sources to enable exercise of professional judgment in modern ethical issues.
c. Students will be encouraged to think critically about the ethical implications of what
medical practitioners do.
2. Specific:
a. Knowledge:
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i. To understand the values and principles concerning ethics and personality
development.
ii. To comprehend the basic concepts, ideas and principles about professional and
scientific issues.
iii. To exhibit leadership qualities in ethic and profession.
b. Skills:
i. To analyze ethical issues and resolve the problems arise in their profession.
ii. To be able to understand and implement the Islamic ethical values in the field of
Medical Science.
c. Attitude/Behavior:
i. To develop honest and responsible professionals who will be able to perform
leading role in their respective fields.
Educational Methodology to be used:
1. Interactive Lecture
2. Discussion
3. Demonstration
Course Contents:
1. General Ethics
a. What is ethics? In General an introduction.
b. The development of ethical theory.
c. The basic principles of ethics in different religions.
d. Ethics in Islam, a comprehensive view.
e. Moral conduct and Faith.
f. The five pillars of Islam and moral conduct.
g. The moral qualities of believers.
h. Moral conduct and Taqwa.
i. The distinction of Prophet Muhammad (SAW) among the teachers of moral conduct.
j. Impact of Role Model on personality
k. Virtuous conduct and Baseness (Raza’il)
l. Qualities of a good professional
m. Rights and duties
n. Etiquettes and manners
2. Scientific Issues:
a. The process of expansion of universe.
b. Beginning of Life. Evolution Stages & End
c. Truth about Big Bang Theory (Creation of Universe).
d. Purpose of Creation of Mankind & Who Created it
e. Revealed Sciences.
f. Difference between Creator & creatures, a comparative study
g. Law of Conservation of Matter
h. Balance
i. Education
3. Bio-Medical Ethical issues
a. Dissection on dead bodies
b. Theory of Evolution
c. Genetic engineering
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d. Postmortem
e. Cloning, therapeutic/reproductive and Parthenogenesis.
f. Stem cell engineering.
g. Surrogate mother.
h. Medical aspects and moral implications of Rape.
i. Ancient Medicine and Surgery
j. Tib-a-Nabavi (SAS)
k. Muslim contribution to Medicine and Surgery
l. Modern medicine
m. Infertility and its ethical an psychological effects.
n. Organ Transplant and donation
o. Cosmetic Surgery
p. Commercial use of human remains
q. Gender selection
r. Deformation
s. Abortion
t. Population Planning
u. Code of ethic in social life to protect from extremism & Clashes.
v. Medical profession as a mission of curing not only physical problems but also treatment
of whole human, including spiritual and moral problems.
w. Sexual misconduct, obscenity; disrespect for relations.
x. Persecution & Human Right
y. Embryological Development of Reproductive System.
z. Bribe
4. Some Common and popular myth
a. Magic, its viability in nature.
b. Unidentifiable objects
c. Dreams and their nature an effects on behavior.
d. Ghost. Super Natural & Sprits
e. Telepathy, Horoscope, Hypnosis, Psychological Treatments.
f. E.S.P
g. Telepathy
h. Talismans
Assignment/Projects:
Criterion:
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Inadequate introduction, Captivating introduction,
Low level critical thought and High level critical thought and
analysis analysis
Technology/Equipment/Models/Physical Support:
1. Multimedia
2. Overhead Projector
3. White Board
4. Movies
5. Cassettes
6. CDs
Recommended Text Books:
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Recommended Reference Books:
1. Kasuli, Hassan Omar, Prof. Dr. Lectures notes on Islamic Principles in Medical Practice, IIMU
2. Bashmil, Ahmed, Takamul
3. Chadwick, Ruth, Ethics of New Technologies.
4. Dar, Bashir Ahmed, Ethics in Qur’an, Islamic Book Depot, Karachi
5. Sewharvi, Hifz-ur-Rehman, Ikhlaq aur Falsafa-e-Ikhlaq, Dar-ul-Isha’at, Karachi
6. Al-Ghazali, Shaikh Muhammad, A Muslim’s Character, Dawah Academy, Islamabad.
1. Organizing work
Personnel management
Work flow-scheduling and time management
Computer operations
Record keeping
2. Management/Laboratory Administration
budget development
cost analysis of Reagents
Equipment maintenance
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3. Staffing
f. Leadership
g. Training
d. Performance evaluation
e. In-service education
f. Competency testing
4. Controlling
Inventory
Purchasing/Cost effectiveness
Laboratory accreditation standards
_____________________________________________
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Table of specification
Semester-1
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Anatomy-1 10 10
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Course Code RLT-602
Course title Basic Physiology -1
Credit hours 3(2+1)
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Basic Physiology -1 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
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Practical
Sr. No Topic OSPE Viva voce
1 Basic Biochemistry -1 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Basic lab principles lab safety and 10 10
first aid
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Course Code RLT-605
Course Title Functional English (English -1)
Credit Hours 3(3+0)
Practical
Sr. No Topic OSPE Viva voce
1 10 10
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Semester-2
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Anatomy-II 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
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Practical
Sr. No Topic OSPE Viva voce
1 Basic Physiology -II 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Basic Biochemistry -II 10 10
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Course Code RLT-610
Course Title Basic Chemical Pathology
Credit Hours 4(03+01)
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 10 10
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Practical 20
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
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Course Code UR-250
Course Title Life and living
Pakistan Studies
Credit Hours 2(2+0)
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Pathology practical 10 10
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Course Code RLT-616
Course Title Basic Hematology
Credit Hours 4(03+01)
Paper -I: MCQs (20 Marks)
Marks 20
Paper-II: Answer Questions (SAQs/SEQs) (2 hours, 40 min)
Marks 20
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
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Course Code RLT-618
Course Title English -III
Credit Hours 3(3+0)
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
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Course Code RLT-620
Course Title Basic histopathology
Credit Hours 4(03+01)
Paper -I: MCQs (20 Marks)
Marks 20
Paper-II: Answer Questions (SAQs/SEQs) (2 hours, 40 min)
Marks 20
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
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Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
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Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
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MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
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Course Code RLT-627
Course Title Biostatistics and research methodology
Credit Hours 2(02+0)
Paper -I: MCQs (20 Marks)
Marks 20
Paper-II: Answer Questions (SAQs/SEQs) (2 hours, 40 min)
Marks 20
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
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Course Code RLT-629
Course Title Histopathology Instruments / Techniques
Credit Hours 3(02+01)
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
MCQs SAQs
Topics/ area C2 C3 C2 C3
Interpretation Application Interpretation Application
15 5 2 2
Practical
Sr. No Topic OSPE Viva voce
1 Practical 10 10
Practical
Practical Viva
Practical (1 hour) (35 marks) 25
____________________________________________________________________