 Escherichia coli and Citrobacter

EMB= deep purple colonies with greenish metallic sheen (+) swarming colonies
Maconkey= rose pink colonies (LF) Specific smell= grape like odor/ corn tortilla-like odor

O157:H7 Esch coli= Highly selective SMac Agar(light pink)

Smac agar=Mackoncey Sorbitol Agar Pseudomona pigments:
→do not ferment sorbitol Pyocyanin Turquoise blue/blue-green King’s A medium
=light pink cuz (-) fermentation; others= yellow(+) (H2O soluble, CHCl3 (Pseudomonas P
extractable) agar)
 Enterobacter- fish-eye colonies=pink mucoid colonies with central Pyoverdin/ Yellowish-green King’s B medium
purple dot; Fluorescein (H2O soluble, not CHCl3 (Pseudomonas F
(+) capsule extractable) agar)
Enterobacter aerogenes- aerogenic organism; abundant gas Pyorubin/ Red/brown colored pigment
production Pyomelanin

 Proteus= foul-smelling  Alcaligenes faecalis= Non-fermentative bacilli

(+) swarming colonies (flagella)
Fruity odor (apples and strawberries)

Klebsiella= large pink mucoid colonies (+) capsule pH 7.5

Klebsiella pneumoniae- Friedlander’s bacilli Pseudomonas aeroginosa has:

 Serratia marcescens-large red mucoid colonies (+) capsule Enteric property = (+) for glucose
Arabinose (-) Non-fermentative property = (-) for glucose property
Red cuz of Prodigiosin
- Best at RT(22-25 C) Reading Interpretation
- Non-soluble to water Acid slant over acid butt Glucose, Lactose, Sucrose were
- Red color (use cotton swab) Hydrogen Sulfide not produced fermented
Gas not produced
 Salmonella typhi= jet black colonies (+) H2S
Collective form for infection- Salmonellosis
Specific- Typhoid fever

Weadle test= serologic testing *Cysteinase enzyme= responsible for H2S production

 Pseudomonas aeroginosa
-most common non-fermentative bacilli
- causes HAI =nosocomial infections
Folliculitis-infection of hair follicles
Octitis externa- “swimmer’s ear infection”
Keratitis- contact lenses
→tears contain Stimuloglobulin-a