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By Katie Mark
L-citrulline, a non-protein amino acid, is made by the body and used as a supplement for exercise
performance because of cardiovascular effects optimizing blood flow. In the body, citrulline is
converted to L-arginine (another amino acid), which produces nitric oxide (NO), a small molecule
that dilates blood vessels to allow working muscle to receive more oxygen and nutrients, and remove
metabolic waste. This optimized blood flow may help performance and accelerate recovery.
Studies suggest citrulline may benefit exercise. However, there’s no recommendation for citrulline
intake1, which means the appropriate amount of citrulline (g/day) to take and when to take it (before
exercise or in the short term) is unclear. Therefore, studies investigating citrulline and exercise use
different dosages and supplementation procedures.
Therefore, citrulline intake leads to more arginine and increased production of NO. There must be
enough citrulline intake to increase arginine levels so that NO synthesis can occur. Citrulline blood
levels (plasma) are the main barriers in the conversion of citrulline to arginine results.4
Citrulline intake leads to more arginine and increased production of nitric oxide. CLICK TO
TWEET
Why not supplement with arginine? Arginine supplementation increases plasma arginine, but
citrulline supplementation increases plasma arginine for a longer period of time. In fact, citrulline is
more efficient at increasing arginine levels than arginine itself because arginine is mostly used by the
liver, and citrulline is available for the whole body. Also, arginine supplementation of at least 10g
can lead to diarrhea. Currently, citrulline doesn’t have any side effects, so it’s a preferred source
over arginine.
Citrulline also is part of the urea cycle; an important pathway that removes ammonia from muscle
and the liver.
Plasma arginine increased to a similar extent with arginine and citrulline supplementation,
but plasma citrulline only increased with citrulline supplementation.
Plasma nitrite (another substrate for NO) increased with arginine and increased with
citrulline.
Blood pressure was only lower with citrulline supplementation.
Citrulline improved tolerance to severe-intensity exercise and the total amount of work
completed.
Arginine did not affect blood pressure or performance.
The study concluded that short-term citrulline supplementation (6g/day for seven days) may improve
blood pressure and exercise performance.
Another study found improved blood flow after increasing NO, following 5.6g/day of citrulline for
seven days. The improved blood flow correlated with increases in arginine levels.6
Further support of a dose of 6g citrulline (0.08g/kg) given to pre-professional male cyclists two
hours before exercise found a 173% increase in plasma citrulline, a 123% increase in plasma
arginine, and a significant increase in NO production.7 Creatinine also increased, which suggested
that increased arginine may lead to creatine synthesis. This same dose (0.08g/kg) and a similar
increase in citrulline and arginine levels were also found in 17 male professional cyclists.8
However, two other studies used a slightly higher amount of citrulline, 0.18g/kg (~12g of citrulline
for a 150lb person). One study gave five equal dosages of citrulline at three-hour intervals within 12
hours to increase circulating levels of citrulline, which increased citrulline six times its original
amount and doubled plasma arginine levels.9 The other study found an increase in plasma arginine
and an elevenfold increase in plasma citrulline.10 Therefore, citrulline supplementation can be used to
increase citrulline and arginine availability in the body.
Effect on Exercise
A study investigated the effect of different citrulline dosages and loading protocols on incremental
treadmill tests to exhaustion after citrulline supplementation (9g 24 hours before test or 3g three
hours before test).11 Citrulline ingestion over 24 hours reduced time to exhaustion by 0.8% compared
to citrulline supplementation three hours prior.
Citrulline supplementation of 2.4g/day for seven days and 2.4g of citrulline one hour before a 4-km
cycling time trial reduced completion time by 1.5% in trained males.11 Citrulline also improved
feelings of muscle fatigue and concentration immediately post-exercise.11
An 8g dose of citrulline has shown to increase resistance exercise performance in males,12,13 , but due
to physiological difference between sexes, the results could not be applied to females. Therefore,
two recent studies have investigated citrulline in females.
One study found an increase in upper and lower body resistance exercise performance and lowered
rating of perceived exertion during upper-body exercise when 8g of citrulline was taken one hour
before.14 Another study found that one hour after consumption of 8g of citrulline, there was an
increase in maximal and average grip strength and peak and explosive power in female master-level
tennis players, which was suggested to improve tennis-match play.< sup>15
Another study providing 6g/day of citrulline for 22 days found reduced reports of fatigue from men
who usually complained of fatigue.17
The study concluded that saturation of citrulline begins to occur at 15g, and 10g may be an
appropriate amount to use. This lower amount supports another study that found 12g of citrulline
taken one hour before a time-to-exhaustion test on the bike did not provide ergogenic benefits in
well-trained males.19
It’s unclear how much citrulline we need. Even though there’s no recommendation on citrulline
intake, it’s suggested that a 70kg person should be able to tolerate a daily dose of arginine from a
normal diet (4-6g/day).20 Therefore, the dose of 6g of citrulline could be the same as the daily intake
of arginine.
Moreover, maybe the amount should be provided in grams per kilogram of body weight (similar to
how protein requirement is determined to take into account larger and smaller athletes). If so, it
appears that citrulline supplementation may be 0.08-18 g/kg/day.
The amount needed must also consider citrulline bioavailability, which may be limited by intestinal
absorption.21 Citrulline bioavailability is higher when contained in its natural form (e.g.,
watermelon), but further information on this is out of the scope of this article.
It’s also unclear when to supplement with citrulline (e.g., 1-2 hours before exercise or days prior to
exercise).