Name: Hastaning Asri_18109011037_8_2

Exercise 8: Chemical and Physical Processes of Digestion: Activity 2: Exploring Amylase Substrate Specificity Lab Report

Pre-lab Quiz Results

You scored 100% by answering 5 out of 5 questions correctly.

1. Which of the following is true of enzymes and substrates?

You correctly answered: c. Enzymes are specific about the substrates they can act upon.

2. Which of the following is/are reducing sugars?

You correctly answered: e. both glucose and maltose

3. Cellulose and starch are both

You correctly answered: e. polymers of glucose and polysaccharides.

4. Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide bond.
Which of the following enzymes do you think would be specific for a peptide bond?
You correctly answered: c. peptidase

5. The Benedict's assay tests for the presence of

You correctly answered: c. reducing sugar.

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Experiment Results
Predict Question:
Predict Question: Do you think test tube 3 will show a positive Benedict's test?
Your answer : b. no

Stop & Think Questions:

You included tube 2 to
You correctly answered: b. see what a positive Benedict's test should look like.

Which tube is included to detect contaminating amylase in the buffer or in cellulose?

You correctly answered: d. tube 4

What is the usual substrate for the pancreatic enzyme peptidase?

You correctly answered: d. peptides

Experiment Data:

Tube No. Reagent 1 Reagent 2 Reagent 3 Time Temp. IKI Benedict's

1 Amylase Starch pH 7.0 Buffer 60 37 - ++
2 Amylase Glucose pH 7.0 Buffer 60 37 - ++
3 Amylase Cellulose pH 7.0 Buffer 60 37 + -
4 Cellulose pH 7.0 Buffer Deionized Water 60 37 + -
5 Peptidase Starch pH 7.0 Buffer 60 37 + -
6 Bacteria Cellulose pH 7.0 Buffer 60 37 - ++

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. The substrate(s) for amylase is/are

You correctly answered: a. starch.

2. The results of tube 5 demonstrated that

You correctly answered: b. peptidase does not digest starch.

3. Why was the cellulose in tube 6 hydrolyzed to glucose?

You correctly answered: d. The bacterial suspension contained the enzyme cellulase, which digested the cellulose.

4. Can you detect the presence of contaminating amylase from your experiments?
You correctly answered: c. It is not possible to determine whether contaminating amylase is present because amylase
doesn't digest cellulose.

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Review Sheet Results
1. Describe why the results in tube 1 and tube 2 are the same.
You did not answer this question.

2. Describe the result in tube 3. How well did the results compare with your prediction?
You did not answer this question.

3. Describe the usual substrate for peptidase.

You did not answer this question.

4. Explain how bacteria can aid in digestion.

You did not answer this question.

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