172.

Based on the data, which of the following statements best describes the uptake
of sucrose by these cells?
(A) Sucrose crosses the membrane by simple
diffusion.
(B) Sucrose is transported by a carrier-mediated
system.
(C) Sucrose transport is not dependent on Na+.
(D) Sucrose transport occurs by only one
mechanism.
(E) Sucrose is not transported into the cell under
most conditions.

173. The primary reason for testing the effects of CCCP is to determine whether
(A) the effect of Li+ is dependent on Na+
(B) a proton gradient is required for sucrose
transport
(C) Na+ transport occurs alone
(D) nonsaturable transport occurs
(E) K+ can substitute for Na+ in sucrose
transport

174. Which of the following is the best explanation for the effects of Li+ and K+ on
the uptake of sucrose?
(A) These ions bind to sucrose and block its entry
into the cell.
(B) These ions decrease the net Na+ available in
the cells for cotransport.
(C) These ions uncouple the respiratory process
that generates energy for the uptake of
sucrose.
(D) These ions are not transported rapidly by the
Na+/sucrose cotransporter system.
(E) These ions are allosteric effectors of the
sucrose transporter system.

Questions 182-185 refer to the following model as an example of adaptive radiation

and species diversification.

One of the classical examples of evolution occurs on the Galápagos Islands with
Darwin’s finches. The islands have always been separate from the South American
mainland and vary in size and elevation. The lowlands are covered with thorn scrub,
while higher elevations (found only on the larger islands) are covered with moist, dense
forests. All the organisms living on these islands are descendants of species that have
emigrated there, primarily from South America. In studying the finch populations,
researchers have identified fourteen species, none of which are found on the mainland.
Describe the similarities and differences in the cycling of Gs
and Ras between the active and inactive forms.

2. Some integral membrane enzymes depend upon the lipids in their

microenvironment not only for scaffolding, but for enzymatic activity. The Na+-K+
ATPase is one example. Please design an experiment to test the influence of
membrane fluidity on the velocity of the Na+-K+ ATPase.

3. a. The average molecular weight of proteins in the cell is about 30,000 daltons. A
few proteins, however, are very much larger. The largest known polypeptide chain
made by any cell is a protein called titin (made by muscle cells), and it has a
molecular weight of 3,000,000 daltons. Estimate how long it will take a muscle cell
to translate an mRNA coding for titin (assume the average molecular weight of an
amino acidto be 120, and a translation rate of two amino acids per second for
eucaryotic cells).
b. Transcription occurs at a rate of about 30 nucleotides per second. Is it possible to
calculate the time required to synthesize a titin mRNA from the information given
here?

4. What is the advantage of having an extracellular signal transmitted by a cascade

of sequential events?

5. Heating of calf type I collagen fibers to 450C denatures the triple helices and
separates the three chains from each other. Collagen that has been treated in this
way does not renature to form a normal collagen triple helix. Why?

i all Biotecknika memebers,

I am having some questions & I shall be very thankul if anyone among u is able to
answer any among the following questions and able to clear my doubts.

• I wanted to know if companies like Dabur , Panacea , Biocon etc provide

oppportunities for us to do part -time Ph.D in their organizations after MS.c and
if so then what is the procedure for that. If not then what are the other institutes
that provide part time Ph.d programmes and what is the procedure for the same
also is NET essential for doing part time Phd.
• Is Ph.D an essential criteria to enter into a company and to be successful?
• Also, can anyone provide me the sites or samples for preparing best biotech
resumes?
• If anyone among the biotecnika members is doing job in a good biotech company
then please let me know the procedure for the same and also how to apply or what
are the steps to enter into good a company after MS.c Biotech.
• what are the things that one must follow to clear the interviews.
• what other skills in computers do a msc biotech need to have to excel in a biotech
company?
• What are the good magazines related to biotech available in the market to make
ourselves upto date with the currrent biotech topics and issues.
• Are there any good and useful distance learning programmes after msc biotech?
• I know that I am asking many questions all at one time but I shall be very
thankful if anyone is able to answer these and remove my doubts and queries.