Documente Academic
Documente Profesional
Documente Cultură
GESTROI)
In Partial Fulfillment of the Requirements for the Senior High School Program,
Carmela Q. Amparo
March 2020
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 2
ACKNOWLEDGEMENT
First of all, the researchers would like to beholden the Almighty God for giving
them the strength to do this paper. In addition, they would like to give their warmest
gratitude to their Capstone teacher, Mrs. Diane M. Valencina and Practical Research 2
and Inquiries Teacher, Mr. Niel Vincent B. Catapang; for their guidance and help to the
The researchers would also like to give thanks to FAITH Fidelis Senior High for
giving them the permission to conduct the study about the insecticidal potential of chilli
To the family and love ones of the researchers that supports them in making their
research.
The Researchers
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 3
DEDICATION
We dedicate this research work to our school, First Asia Institute of Technology
Also, to our family whose words of encouragement really helped us a lot in the
To our brothers and sisters who boosted our confidence on finishing this research.
We also dedicate this to our teachers, mentors, friends and classmates who never
The Researchers
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 4
ABSTRACT
gestroi)
STRAND STEM
SUMMARY
Datil pepper has many nutritional benefits and is widely used as spice among
Asian countries. It has also the capability to kill insects found in common households.
Keeping in view these facts about Datil pepper and its usability, this study aims to test the
efficacy of Datil pepper fruit extract against wood termites. Grinding and squeezing
method was used to get the juices from the fruit. Fifteen termites were collected from dry
wood. They were then placed into three open containers, sprayed by the extract and
observed within 10 minutes. In container A that has 100 percent concentration of the
Datil pepper extract, termites were killed abruptly in 3 minutes and after 5 minutes,
termites were found dead in container B (75 % of Datil pepper fruit extract and 25%
distilled water). In the span of 6 minutes, termites within container C (50 % of Datil
This part covers the content of chapters 1-3, so please highlight some parts of the
introduction, the main objectives of the study, research locale, the materials and
TABLE OF CONTENTS
PAGE
TITLE PAGE …………………………………………………………………... i
ACKNOWLEDGEMENT ……………………………………………………… ii
DEDICATION …………………………………………………………………. iii
ABSTRACT ……………………………………………………………….......... iv
SUMMARY……………………………………………………………………… v
TABLE OF CONTENTS ……………………………………………………….. vi
CHAPTER 1
THE PROBLEM AND ITS BACKGROUND
Introduction ………………………………………………………… 1
Assumptions………………………………………….......................... 3
Significance of the Study …………………………………………..... 3
Scope and Limitation ……………………………………………….. 4
Definition of Terms ………………………………………………….. 6
CHAPTER 2
REVIEW OF LITERATURE
Conceptual Literature …………………………………………......... 7
Research Literature………………………………………………….
12
Synthesis…………………………………………………………
13
CHAPTER 3
METHODOLOGY
Research Design ………………………………………………………………... 14
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 7
REFERENCES………………………….………………………… 21
APPENDICES
Letter to the Validators………………………………………………. ix
Letter to the Registrar……………………………………………....... xi
Letter to the Principal…………………………….……………….. xiii
LIST OF FIGURES
CHAPTER 1
This chapter discusses the introduction that includes the rationale, conceptual
framework, assumptions, and significance of the study, scope and limitations, and
definition of terms.
Introduction
Pesticides have been utilized worldwide to prevent and manage unwanted pests.
They are important for disease control and providing food and fiber for a growing world
population. With the introduction of pesticides, farmers have been able to ensure
bountiful harvests and reduce labour. Pesticides are therefore an indispensable tool for
the sustainable production of high quality food and fibers. Synthetic pesticides leave
harmful residues in crops for human consumption increased the interest in using organic
products for pest control. These toxic residues are significant and cannot be dismissed
Several researches in the Philippines were made to address insects. Some of these studies
In this study, the researchers will utilize a Datil pepper or Capsicum chinense, a
species of habanero-type chili peppers. In comparison to the jalapeno peppers, the Datil
pepper is said to be 12 to 120 times hotter (Southern Kitchen, 2019). It possesses the
chemical capsaicin which might prove to be useful against termites. The active
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 10
component of Datil pepper is a natural chemical that is often found on animal repellents
Filipinos are aware that Termites are one of the common problems in some
households as they cause the destruction of any furniture and shelter made of wood. They
are often referred to as the “white ant” because of their size and colour. Coptotermes
gestroi, the Asian subterranean termite is a small species of termite that lives
underground. This termite is also known as the Philippine milk termite (Coptotermes
gestroi). There are two major methods of termite control. You can use liquid termite
insecticides (termiticides) for barrier and soil treatment or use termite baits. Both of these
methods work on exterminating the termites, but the termiticides have been known to
This study aims to find out the effectiveness of Datil (Capsicum chinense) pepper
extract as an insecticide that can kill termites (Coptotermes gestroi). Using similar studies
that utilized chilli peppers, this study also aim to compare its results to other chilli pepper
insecticides. In addition, several studies have indicated the potential ecological damage
due to the widespread use of inorganic pesticides. Hence, this study may prove to be an
essential part for a further study about the development of an organically produced
This study aims to determine the effectiveness of the Datil pepper extract against
termites.
2. To test the effectiveness of the Datil pepper (Capsicum chinense) fruit extract
chinense) fruit extract and the chilli pepper (Capsicum frutescens) fruit extract.
Assumptions
1. The study assumes that Datil pepper insecticide can annihilate termites.
2. A Datil pepper insecticide is more effective than other type of chilli pepper
insecticide.
3. Insecticides that are made of Datil pepper are more effective than commercial
insecticides.
The researchers are confident that this product research can contribute to the existing
body of research and improve the quality of life. It is important to the following:
Consumers. The information indicated will be significant to the consumers because this
product can prevent diseases and complications on their caused by the harmful effects of
inorganic insecticides.
Farmers. The study can help develop an insecticide that is low-cost and environment
friendly, increasing their profits. Insecticides increase farm profits by helping the farmer
Future Researchers. The study can be used as basis to future studies about insecticides
and will also help future researchers to study the effect of Datil pepper to other insects.
The study focuses on the effectiveness of the Datil pepper extract as an insecticide
against termites. On the other hand, the study will not cover the other factors that lead to
the results of the experiment. The data will be collected through observation by the
laboratory. Moreover, techniques involve the correct method in order for the equipment
to work according to their use like grinding, transferring and measuring. Equipment can
be made from recyclable materials which can be found on homes or to the laboratory
equipment.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 13
Conceptual Framework
PROCESS OUTPUT
arrive with an effective output or product. First, the researchers will obtain the Datil
pepper fruit and the termites to be used in the experiment. Second, the grinding and
squeezing method will be used to get the extract from the Datil pepper. The termites were
the extract and observed within 10 minutes to take effect. Lastly, the results were then
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 14
evaluated to prove the effectiveness of the Datil pepper fruit extract insecticide using an
observation sheet.
Definition of Terms
experiment as a pesticides to help preventing the maximum amount of pest that destroys
Insecticides- are substances used to kill a specific type of insect. It includes ovicides and
larvicides that have the capacity to dispatch insect eggs and larvae, respectively.
insecticide. Like other chlorinated aromatic hydrocarbons, DDT tends to persist in the
environment and become concentrated in animals at the head of the food chain. Its use is
species of termite that lives underground. Both this species and the Formosan
subterranean termite, are destructive pests native to Asia, but have spread to other parts of
Pesticides- are substances that are meant to control pests, including weeds. The term
CHAPTER 2
This chapter presents the related literature and studies that helped the researcher
to supports the concepts of the study. It also aims that established literature and studies
have existing ideas, which assist to answer the problems that the researcher wanted to
solve.
Conceptual Literature
farmers that utilizes the plant’s natural defences against insects. It may not be only
inexpensive, but also safer and more environmental-friendly. Some plants have their own
natural insecticide within them to fend off insects, many plant species produce substances
that protect them by killing or repelling the insects that feed on them. For example, the
Douglas fir has a special sap that fends off beetles if it is attacked. Neem trees secrete oil
that changes the hormones of bugs so that they cannot fly, breed or eat (Petruzuello,
2018).The Datil pepper (Capsicum chinense) contains the active chemical capsaicin, the
chemical responsible for making it spicy, works well against insects. Most insects will
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 16
avoid capsaicin because it has such a strong odor and taste. It also irritates their skin,
According to Kaila (2011), Capsaicin at 10 parts per million causes a persistent burning
sensation. Capsaicin works by opening the gaps in the cell membranes that enable
calcium ions to flood into the cell, making it trigger a pain signal that is transmitted to the
Moreover, we can measure the hotness of a chilli using Scoville Scale. The
Scoville Scale can be used to not only measure chili peppers, but anything that is made
from chili peppers, such as hot sauce. What is really being measured is the concentration
of “capsaicin“, the active ingredient that produces that sensation of heat on our tongues.
The term “capsaicin” comes from the pepper plants’ classification, of the genus
Capsicum. Capsaicin occurs naturally in peppers along with other capsaiciniods, all of
which make up the unique tastes and heat reactions of each pepper, depending on their
ratios.
Below is a list of Scoville Heat Units (SHU) of the most common chili peppers:
Datils are quite hot by any chili pepper standard. They measure from 100,000 to
300,000 Scoville Heat Units on the Scoville Scale, placing them in the territory of both
habanero peppers and Scotch Bonnets. Compare that to the more common jalapeno
pepper, which averages around 5,000 Scoville Heat Units, and you’ll find the datil pepper
furniture, building, or anything made of wood. They are small white insects which live in
hot countries. Termites do a lot of damage by eating wood. According to Ring & Wilson
(2016), termites cause more damage to homes than all other natural disasters combined.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 18
They are often referred to as the “white ant” because of their size and colour. Nicknames
such as “the little white thing” or “little white ant” come up in discussing this insect. This
termite is also known as the Philippine milk termite (Coptotermes gestroi). Modern
houses make extensive use of concrete slabs, and for most effective termite proofing
much of the chemical barrier needs to be put under these slabs (Aguhob et al., 2012).
Insecticides
(2016), insecticides kill by affecting the physiology of the insect. It abrades the skin of
the insect, causing them to lose water. They are classified based on their structure and
mode of action. Many insecticides act upon the insect's nervous system (e.g.,
support with this, a study conducted by Benson and Olufunke (2011) found low levels of
because the does not have the proper mechanisms to remove it. Many synthetic pesticides
are not able to be broken down. Once they enter the body of organism, they are
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 19
permanently stored in the body tissue. In relation to this, a study conducted by Yu et al.
of toxic residues within them. The results of this study showed that DDTs were the most
abundant residue in the dolphins, followed by polychlorinated biphenyl (PCB) and other
organochlorine pesticides.
Research Literature
The Datil pepper fruit extract has the ability to kill termites because insects tend
to avoid plants with strong odor or taste, killing or repelling them away. Moreover, some
studies suggest that capsaicin based insecticides have the same effects to other insects
like mites and ants. According to the National Pesticide Information Center (2009), the
capsaicin present in the Datil pepper causes the membrane of the insect’s cells to suffer
In support with this study, a research made by Cuadrado et al. (2019) entitled
to test its insecticidal effects on aphids (Aphis cytisorum). Under laboratory conditions,
they found out that both capsaicinoids and glucosinolates have biocidal effect on aphids
in just a shot amount of time. Thus, it is proved that the capsaicin present on the Datil
Oleifera) and Chili fruit (Capsicum Frutescens) as Pesticide was conducted to observe
whether the mixture of malunggay and chilli extract would work against cockroaches.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 20
The researcher used commercial pesticide to compare its effectiveness to the mixture of
malunggay and chilli extract. The extract was effective against the cockroaches but the
commercial pesticide was more effective. It is therefore concluded that the extract of
A similar study by Colon et al. (2016), found out the effectiveness of Siling
Labuyo (Capsicum frutescens) fruit extract against Philippine Milk termites (Coptotermes
however, in container B that has 100 percent concentration of the extract, termites were
killed in the span of 5 minutes and after two hours, termites were dead in container C (50
% of chili pepper fruit extract and 50% distilled water). Therefore, the fruit extract of
Synthesis
The information gathered from the related literature convinced the researchers to
believe that their proposed study is plausible. It began by providing the background of the
variables present in the study namely the Datil pepper (Capsicum chinense), the
Philippine Milk Termite (Coptotermes gestroi) and the definition of insecticides and its
types. Succeeding that, it stated studies that directly support the study. The researchers
aim to help produce a capsaicin-based product that can substitute or replace commercial
products that are harmful for the environment. Based on the studies cited and discussed,
the chilli pepper fruit extract has the ability to kill different types of insects, including the
specific species of termites that we need for this study. It has been proven across previous
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 21
evaluations against insects, whether it is used in a mixture or used pure. Therefore, the
researchers are confident that the data gathered throughout this study are sufficient in
supporting their study. They collected a fair amount of studies and experiments made by
researchers and scholars pertaining to the Chili pepper (Capsicum chinense) and its
CHAPTER III
METHODOLOGY
This chapter presents and discusses the design and methods employed in
conducting this research study. It has five parts: research design, subject of the study,
data gathering instrument, data gathering procedure and the observation. Each topic was
Research Design
research is any science-based study in which a number of variables are kept constant
while the other set of variables are calculated as the focus of the experiment. Researchers
research can collect a lot of data that can help in making better choices (QuestionPro,
2020). This method of design allows the researchers to establish casual interpretations of
differences in other factors are calculated by the use and regulation of one or more
variables.
This study included the termites (Coptotermes gestroi) as the dependent factor
and the percentage of solution or the Datil Pepper (Capsicum chinense) extract as
independent variable.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 23
The researchers believe that this is the most appropriate design to be used in the
study because it portrays the most appropriate technique to demonstrate the solution to
This experimental study was conducted at FAITH. The researchers used termites
(Coptotermes gestroi) and Datil Pepper (Capsicum Chinense) as the subjects of the study.
Fifteen termites were gathered from dry wood to serve as test subjects. The test subjects
were checked whether they belong to the species the researchers required through
verification by the University of the Philipines Los Banos Natuural History Museum.
The information was gathered through observing the termites’ response to the
Datil Pepper (Capsicum Chinense) fruit extract as an insecticide. The researchers used an
type of organized instrument form used to record data obtained by closely observing
different samples. It also included the actual time and the duration of the trial and
conclusions. All the data were gathered and tabulated to ensure the accuracy of the
results.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 24
The following methods were observed and employed to evaluate the experimental
trials:
A. PREPARATION OF MATERIALS
The materials used in the study were Datil pepper extract and distilled water. In
addition the researchers also used termites to test the efficiency of Datil pepper extract.
These insects are gathered from Tanauan City, Batangas were the experimentation was
conducted. The researchers chose this locale because of its suitability for the requisite
B. PREPARATION OF EQUIPMENTS
To successfully test the solutions (100%, a number of equipment were used in the
experiment. Most of the equipment was bought from different stores in Tanauan City,
Batangas.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 25
completely get the extract from Datil pepper; measuring cup and dropper for
measurements; and spacious containers where the insects and Datil pepper extract were
placed.
The stirring materials, strainer, and measuring cup were placed in a container with
hot water for ten minutes. An autoclave was used to sterilize the equipment and to
To extract from Datil pepper, the Datil peppers were washed thoroughly and
grinded using a blender. The grinded pepper was then squeezed through a strainer to
get the extract of the chilli pepper.
The first solution was composed of 5ml of pure Datil pepper extract. The
second solution was composed of 5ml of Datil pepper extract mixed with 2.50 ml of
distilled water. The third solution was made up to 5ml Datil extract and 5 ml of
distilled water.
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 27
When the extraction was done, the researchers prepared the insects for the
experiment. The different solutions under this factor were evaluated: termites
(Coptotermes gestroi) reaction. The researchers examined how efficient the Datil pepper
References
Aguhob, W.A., Lumasag, J., & Romano, C.I. (2015).The Insecticidal Potential of Chili
sciece.com/2012/11/theinsecticidal-potential-of-chili.html
from https://www.ajol.info/index.php/ajest/article/view/71959/60916
in Indo-Pacific humpback dolphins (Sousa chinensis) from the Pearl River Estuary,
https://www.sciencedirect.com/science/article/pii/S0045653514005050
https://homeguides.sfgate.com/homemade-chili-powder-sprays-insecticide-
86195.html
https://www.amnh.org/learn-teach/curriculum-collections/young-naturalist-
awards/winning-essays/2011/plant-extracts-as-natural-insecticides
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 29
https://www.britannica.com/plant/neem-tree
Ring & Wilson. (2016): Insecticides for Termite Control. Retrieved from
http://www.lsuagcenter.com/portals/our_offices/parishes/ouachita/features/anr/e
ntomology/insecticides-for-termite-control
Southern Kitchen. (2019). Get to know Florida's datil pepper hot sauce. Retrieved from
https://www.southernkitchen.com/articles/eat/datil-peppers-and-the-unique-
culinary-scene-of-st-augustine?
utm_source=affiliate&utm_medium=314743&utm_campaign=1076744
The Feasibility of Malunggay Leaves (Moringga Oleifera) and Chili Fruit (Capsicum
https://fushiknights.blogspot.com/2009/04/investigatory-project_02.html?m=1
https://www.epa.gov/caddisvol2/insecticides?
fbclid=IwAR1EFZsdRtxdbrG4gYnbHko-a89x6A73E92n-qYEysWHFDwIfkIo-
8RjvWc
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 30
APPENDIX A
Dear Ma’am:
Greetings of peace!
We, the students from Science, Technology, Engineering, and Mathematics strand, are
conducting research paper entitled, “Testing the Insecticidal Potential of Datil Pepper
(Capsicum chinense) Fruit Extract against Termites (Coptotermes Gestroi)”. In
partial fulfillment of the requirements in Practical Research II, we would like to request
for an official count of Grade 12 Fidelis Senior High student per section this school year
2019-2020.
We are looking forward to your response. Rest assured that the gathered information will
be kept highly confidential. Thank you very much and God bless.
Respectfully yours,
CARMELA Q. AMPARO
CHRISTINE JEAN O. CALOS
EARL JUSTIN R. COSMOD
JOHN ANGELO S. DELAS ALAS
JEJOIA PETER S. TANCHANCO
Contact Number: 09076064421
APPENDIX B
Dear Ma’am:
Greetings of peace!
We, the students from Science, Technology, Engineering, and Mathematics strand, are
conducting research paper entitled, “Testing the Insecticidal Potential of Datil Pepper
(Capsicum chinense) Fruit Extract against Termites (Coptotermes Gestroi)”. In
partial fulfillment of the requirements in Practical Research II, we would like to request
for an official count of Grade 12 Fidelis Senior High student per section this school year
2019-2020.
We are looking forward to your response. Rest assured that the gathered information will
be kept highly confidential. Thank you very much and God bless.
Respectfully yours,
CARMELA Q. AMPARO
CHRISTINE JEAN O. CALOS
EARL JUSTIN R. COSMOD
JOHN ANGELO S. DELAS ALAS
JEJOIA PETER S. TANCHANCO
Contact Number: 09076064421
APPENDIX C
Letter for the Principal
Greetings of peace!
In partial fulfillment of the requirements for the subjects Practical Research II and
Inquiries, we, the students from Science, Technology, Engineering, and Mathematics
strand, are currently working on a quantitative research entitled, “Testing the
Insecticidal Potential of Datil Pepper (Capsicum chinense) Fruit Extract against
Termites (Coptotermes Gestroi)”. In lieu of this, we would like to ask for your
permission to allow us to conduct a survey on Grade 12 Fidelis students. Once approved,
an informed consent will be given to the respondents before the actual data gathering.
We are looking forward to your response regarding this matter and we rest assured that
the information gathered for this study will be handled with utmost confidentiality. Thank
you very much and God bless.
Respectfully yours,
CARMELA Q. AMPARO
CHRISTINE JEAN O. CALOS
EARL JUSTIN R. COSMOD
JOHN ANGELO S. DELAS ALAS
JEJOIA PETER S. TANCHANCO
Contact Number: 09076064421
Curriculum Vitae
09076064421
imejroxascosmod@gmail.com
Personal Information
Gender: Male
Age: 19
Weight: 46 kgs.
Height: 5’4”
Academic Background
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 35
2018-2020
2014-2018
Primary
2007-2013
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 36
Curriculum Vitae
Malvar, Batangas
09075631403
S2018102836@firstasia.edu.ph
Personal Information
Gender: Female
Age: 17
Weight: 75 kgs.
Height: 5’4”
Academic Background
2018-2020
Malvar, Batangas
2014-2018
Primary
Malvar, Batangas
2008-2014
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 38
Curriculum Vitae
09662295254
angeloalas05@gmail.com
Personal Information
Gender: Male
Age: 18
Weight: 78 kgs.
Height: 5’7”
Academic Background
2018-2020
2014-2018
Primary
2008-2014
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 40
Curriculum Vitae
09100952550
platbelike@gmail.com
Personal Information
Gender: Male
Age: 18
Weight: 55 kgs.
Height: 5’4”
Religion: LDS
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 41
Academic Background
2018-2020
2014-2018
Primary
2008-2014
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 42
Curriculum Vitae
Amparo, Carmela Q.
09554161764
camilleamparo16@gmail.com
Personal Information
Gender: Female
Age: 19
Weight: 50 kgs.
Height: 5’3”
Academic Background
2018-2020
2014-2018
Primary
2008-2014
DATIL PEPPER FRUIT EXTRACT AGAINST TERMITES 44