Anor, Murielle Nikole

Galvez, Kryztine Mae

Gumban, Andrea Celina
CHE142 PS1.1 Enzyme Kinetics

Problem 2.

Solution:
Using Lineweaver-Burk Plot:
For glucose

concentration of initial
Glucose velocity 1/v 1/s

80 0.151 6.622516556 0.0125

100 0.194 5.154639175 0.01

300 0.385 2.597402597 0.003333333

500 0.355 2.816901408 0.002

750 0.389 2.570694087 0.001333333

 1000 0.433 2.309468822 0.001

1400 0.445 2.247191011 0.000714286

graph for glucose

7

2
y = 351.68x + 1.9227
1
R² = 0.9718
0
0 0.002 0.004 0.006 0.008 0.01 0.012 0.014

For xylose

concentration of initial
Glucose velocity 1/s 1/v

20 0.32 0.05 3.125

50 0.521 0.02 1.919385797

100 0.699 0.01 1.430615165

300 1.11 0.003333333 0.900900901

500 1.19 0.002 0.840336134

700 1.33 0.001428571 0.751879699

 900 1.13 0.001111111 0.884955752

graph for xylose

3.5

2.5

1.5
y = 48.033x + 0.8046
1 R² = 0.9834
0.5

0
0 0.01 0.02 0.03 0.04 0.05 0.06

For glucose
For xylose

Relative catalytic efficiency=0.347/0.047

=7.383
Since the catalytic efficiency of xylose > than glucose, xylose is more favorable substrate than glucose.
Greater is the catalytic efficiency of an enzyme towards a substrate, greater is the substrate specificity
towards that substrate.
Therefore, substrate specificity of the enzyme towards xylose is around 7.383 times greater than that
towards glucose.
Problem 3

Solution:

Temperature
Temperature (K) 1/T (K-1) Rate, k (mmol m-3 s-1) ln k
(˚C)
20 293.15 0.0034112 0.31 -1.17118
30 303.15 0.0032987 0.66 -0.41552
40 313.15 0.0031934 1.2 0.18232
60 333.15 0.0030017 6.33 1.84530

a. Solving for the activation energy, E

The equation for the straight line in the plot below is
where –E/R = -7333.8 K.
We know that the value of R is equal to 8.314 J/K-mol. Solving for E, the activation energy,

Therefore, the activation energy is

1.5 y = -7333.8x + 23.771

R² = 0.9915
1
ln k (mmol m-3 s-1)

0.5

0
0.0029 0.003 0.0031 0.0032 0.0033 0.0034 0.0035
-0.5

-1

-1.5
1/T (K-1)

b. Compare the reaction rate at 55˚C to the temperature of 25˚C

To get the reaction rate at 25˚C, convert the temperature to Kelvin
T = 25 + 273.15 K = 298.15 K
Substitute this value from the equation obtained in part a.

k = 0.4375 mmol m-3 s-1

Doing the same thing for 55˚C
T = 55 + 273.15 K = 328.15 K

k = 4.1457 mmol m-3 s-1

The rate at 55˚C which has a reaction rate of 4.1457 mmol m-3 s-1 has faster reaction rate compared
to the reaction rate at 25˚C.
 c. Compute for the half life of enzyme at 25˚C and 55˚C and determine which is more
practical to use for processing baby food.
At 55˚C = 328.15 K using the given equation in part c

where R is equal to 1.9872 cal K-1 mol-1

kd = 0.42 h-1
the half-life of the enzyme at 55˚C is

At 25˚C = 298.15 K

kd = 6.87 x10-4 h-1

and the half life of the enzyme is

Answer: The enzyme half life at 25˚C is 1009 h longer compared to the half life of enzyme at 55˚C. From
these obtained values, the 25˚C is more practical to use unless certain considerations are needed.