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ARYAKUL COLLEGE OF PHARMACY AND RESEARCH LUCKNOW

B.PHARM IIIrd Year


PHARMACEUTICAL BIOTECHNOLOGY- BP605T
UNIT -1
SESSION 2019-2020

Multiple Choice Questions


Q.1: Which one of the following is the first rDna product:
A. Interlukins B. Leptin C. Insulin D. Tissue of plasminogen activator
Q.2: The double helical structure of DNA is discovered by:
A. Crick and Watson
B. Messlon and Stahl
C. Milstein and Kohler
D. Sanger and Coulson
Q.3: The enzyme not used in genetic engineering:
A. Ligase
B. Polymerase
C. Phosphatase
D. Lipase

Q.4: Who is said to be founder of modern Genetics:


A. Charles Darwin B. Gregor Mendel C. Robert Koch D. August Weismann
Q.5: Penicillin was discovered by:
A. Paul Ehrlich B. Louis Pasteur C. Milstein Kohler D. A. Flemming
Q. 6: Fermentation technique was discovered by:
A. Paul Ehrlich
B. Louis Pasteur
C. Robert Koch
D. A. Flemming
Q.7: Mechanism of DNA replication studied in e.coli by ---------in 1958.
Q. 8: ____________ is the technique of growing plant cells, tissue and organs in an artificial
prepare medium under aseptic conditions.
Q.9: Bioinformatics is an interdisciplinary fields which addresses biological problems using
computational technique-
A. True B. False
Q.10: The process of making number of copies of a gene segment is called as ---------
engineering.

SHORT QUESTIONS:
1. Define Biotechnology.
2. What is enzyme Immobilization?
3. What is protein engineering, give a precise definition.
4. What is the use of Biosensors?
5. Define Biosensors.
6. Give the uses of the following enzymes:
Lipase, amylases, streptokinase, peroxidases and penicillinases.
7. What is rdna technology?
8. What is genetic engineering?
9. Discuss the various applications of enzyme immobilizations

LONG QUESTIONS:
1. What are different application of biotechnology?
2. Give the scope of protein engineering and genetic engineering.
3. Discuss various methods of enzyme immobilization.
4. What are different applications of biosensors?
5. Discuss the role microbes in the pharmaceutical applications?
6. Give five applications of genetic engineering.

ANSWERS OF MCQ
1. C 2.A 3.D 4.B 5.D 6.B 7.Messlon and Stahl 8.fermentation
9. A 10.GENETIC
ARYAKUL COLLEGE OF PHARMACY AND RESEARCH LUCKNOW
B.PHARM IIIrd Year
PHARMACEUTICAL BIOTECHNOLOGY- BP605T
UNIT -3
SESSION 2019-2020

Multiple Choice Questions


Q.1: Antibodies are:
A. Proteins B. Glycoproteins C. Carbohydrate D. Nucleic Acid
Q.2: Antibodies consists of
A. 2 light chain and 2 heavy chain arranged in Y shaped Configuration
B. A light chain and 2 heavy chain arranged in Y shaped configuration
C. 2 Light chain and 1 heavy chain
D. All of these
Q.3: Light and heavy chains are joined by:
A. Covalent B. H-Bond C. Disulphide bond D. Ionic bond
Q.4: Fab stands for:
A. Fragment antibody Binding
B. Fragment antigen binding
C. Fragment antigen or antibody binding
D. Fragment affinity Binding
Q.5 Which of the following is combined vaccine:
A. Hep-B vaccine B. Hib Vaccine C. DPT Vaccine D. Var Vaccine
Q. 6 The antigen binding site of an antibody is:
A. Antitope B. Epitope C. Paratope D. Endotope
Q.7: Active immunity may be gained by:
A. Natural infection B. Vaccine C. Toxoids D. All of these
Q. 8: The process of introduction of weakened pathogen in to human body:
A. Immunization B. Vaccination C. Attenuation D. None of the above
Q.9: A vaccine may be :
A. An antigenic protein B. A weakened pathogen C. Live attenuated pathogen
D. All of these
Q.10: The most common class of antibody involved in type II hypersensitivity reaction are:
A. IgG B. IgM C. IgE D. IgD

SHORT QUESTIONS:
1. Define immunoglobulins.
2. What are hypersensitivity reactions, write its type also.
3. What is MHC, write its application.
4. What is Vaccine? What is the purpose of using vaccine?
5. Draw the structure of antibodies.
6. Write down the types of antibodies with their function also.
7. What is hybridoma technology?
8. Name different bacterial and viral vaccine.
9. What is immunity?
10. What is cellular and humoral immunity?

LONG QUESTIONS:
1. Give the general preparation of one bacterial and one viral vaccine.
2. Discuss about the polio vaccines.
3. What are the application of hybridoma technology?
4. Explain the various hypersensitivity reaction with examples.
5. Name different blood products and various plasma substitute with their preparation
and uses.

ANSWERS OF MCQ
1. B
2. A
3. C
4. B
5. C
6. B
7. D
8. B
9. D
10. A
ARYAKUL COLLEGE OF PHARMACY AND RESEARCH LUCKNOW
B.PHARM IIIrd Year
PHARMACEUTICAL BIOTECHNOLOGY- BP605T
UNIT -4
SESSION 2019-2020

Multiple Choice Questions


Q.1: The size of the inoculum is normally -------% added in to production tank.
A. .1-1 B. 1-10 C. 10-20 D. 20-30
Q.2: Microbial transformations have the following advantages except:
A. It can reduce multistep reaction into one step
B. Reactions may be carried out under mild conditions
C. Reaction are stereospecific and regiospecific
D. It requires more labour and time.
Q.3: Polymerase chain reaction is useful in diagnosis of:
A. HIV B. Fever C. Diabetes D. None of these
Q.4: RT-PCR Stands for
A. Random temperature PCR B. Random template PCR C. Reverse transcriptase PCR
D. Real time PCR
Q.5: Adenine always binds to:
A. cytosine B. Guanine C. Thymine D. None of the above
Q. 6: cdna stands for
A. Complementary DNA B. Chromosomal DNA C. Copy DNA
D. Cohesive DNA
Q.7: DNA is transcribed into RNA in the nucleus and then RNA is translated in the
A. Cytoplasm B. Nucleus C. ER D. Golgi bodies
Q. 8: Functional unit of gene is:
A. cistron B. exon C. mutan D. recon
Q.9: The Pbr322 is first artificial vector developed from:
A. B.subtilis B. Sachhromyces cerrvisicae C. E.coli D. Hepatitis B virus
Q.10: Insulin is made up of ------------amino acids
A. 119 B. 51 C. 86 D. 55

SHORT QUESTIONS:
1. What is fermentation?
2. Draw a neat labelled diagram of fermenter?
3. List different types of fermenter.
4. Write short notes on
(a) Continuous fermentation
(b) Fluidized bed bioreactor
5. What is microbial biotransformation? List two application of microbial transformations.
6. Define conjugation.
7. What is mutation and mutant?
8. Write different types of mutation.
9. What is blotting technique? List the use of blotting technique.
10. Explain the western blotting.

LONG QUESTIONS:
1. Explain the genetic organization of eukaryotes and prokaryotes.
2. Write one example of the following type of microbial biotransformation’s…
(a)Hydrolysis
(b)Esterification
(c)Decarboxylation’s
(d)Oxidations
3. Explain the process of transformation, conjugation and transduction applicable to
microbial genetics.
4. Discuss about various plasma substitute highlighting their features.
5. Define the role of plasmids and transposons.
ANSWER OF MCQ
1.A 2.D 3.A 4.C 5.C 6.A 7.C 8.A 9.C 10.B
ARYAKUL COLLEGE OF PHARMACY AND RESEARCH LUCKNOW
B.PHARM IIIrd Year
PHARMACEUTICAL BIOTECHNOLOGY- BP605T
UNIT -5
SESSION 2019-2020

Multiple Choice Questions


Q.1: When energy depleted elements associated with a proton are accepted by an organic
molecule, the process is called
A. Fermentation B. Respiration C. Anabolism D. Catabolism
Q.2: In the following terms which is not the definition of fermentation process
A. Do not require an electron transport system
B. Use an organic compound as the final electron acceptor
C. Use an inorganic compound as the final electron acceptor
D. Alcohol is formed from sugar
Q.3: Pasteur effect discovered in 1857, is
A. Inhibiting effect of oxygen on the fermentation process
B. Aerating yeasted broth causes yeast cell growth to decrease, while conversely,
fermentation rate increases
C. A and B
D. All of these
Q.4: Which of the following is not a product of fermentation?
A. Lactate B. Oxygen C. Carbon dioxide D. Ethanol
Q.5: The final electron acceptor in lactic acid fermentation is
A. Oxygen B. Lactic acid C. Pyruvate D. NAD
Q. 6: Identify the correct sequence during the industrial production of substances
A. Inoculation, screening, fermentation, downstream processing, removal of waste
B. Screening, Inoculation, fermentation, downstream processing, removal of waste
C. Fermentation, screening, inoculation, removal of waste, downstream processing
D. Fermentation, inoculation, inoculation, removal of waste, downstream processing
Q.7: Production of ____________ occurs in the fermentation of grains
A. Must B. Brine C. Lactic acid D. Carbon dioxide
Q. 8: ____________ is the detrimental effect of microorganisms in the food
A. Generation of flavour B. Process of fermentation C. Spoilage of food D. All of the above
Q.9: Which is not an advantage of the fermented food?
A. Makes the food more digestible B. Increase storage life C. Synthesize vitamins
D. Decrease intestinal microflora
Q.10: Which is not fruit or vegetable based fermented product
A. Wine B. Beer C. Vinegar D. Sauerkraut
Q.11: The applications of fermentation include
A. cereal products
B. dairy products
C. beverage products
D. all of above

Q.12: An airlift fermenter is used for the:


A. an impeller for mixing
B. Air bubbles for mixing the content
C. Differential density for mixing purpose
D. A Sparger for mixing the contents

SHORT QUESTIONS:
1. Draw a typical complete flow sheet of fermentation process.
2. What is Cell Growth Kinetics in fermentation process, why it is important to study?
3. What are the different products we obtained from fermentation process, give at least five
products based on the fermentation.
4. Enlist five important criteria for an ideal nutrition media.
5. List different type of fermenters.

LONG QUESTIONS:
1. Name different blood products and various plasma substitute with their preparation
and uses.
2. Explain various controlled parameters and monitoring devices in a fermenter.
3. Define:
Batch Fermenter
Continuous Fermenter
Fed Batch Fermenter
4. What the different components used in the production of nutrition media for the
fermentation process.
5. Give the ideal characteristics of an ideal fermenter.

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