Biochemistry 3053

GIFT 2
1. Which enzyme is considered [REDACTED]?

2. A solution at pH [REDACTED] contains a weak acid, HA. The pKa of the acid is [REDACTED]. What is [REDACTED]?

3. In mitochondria, what is the expected pH [REDACTED]?

4. When Compound C [REDACTED] Compound D, then Compound D [REDACTED Compound C [REDACTED].

5. When Compound C [REDACTED] Compound D; Compound D [REDACTED] Compound C [REDACTED].

6. What provides the energy [REDACTED] for [REDACTED]?

7. What do cristae in the mitochondria do? (Select all that apply)

8. (True / False) The amino acid [REDACTED] at pH [REDACTED] has [REDACTED] charged groups.
Congratulations! Due to your success in Dr. Daniel’s Biochemistry class. You have been selected by NASA
to do the biochemical write up on a new organism recovered from a Martian probe! (What are the odds?!?)
During your analysis you have discovered a brand new electron transport chain! The organism appears to
use electron transport as its primary source of energy rather than ATP. This leaves you with a couple of
questions to answer:

During your analysis you discover a new electron transport chain based on:

• Ickygreenone + H+ +2e-  Ickygreenol [REDACTED]

• Barsoom + H+ +2e-  Barsool [REDACTED]

9. Which species is the reductant for the proposed ETC?

10. Which species is the oxidant for the proposed ETC?

11. Which series of voltages represents the correct voltages to trace the electron flow for the free energy
calculation?

12. What is the free energy gained from the electron transport chain presented?

13. From one run of the proposed electron transport chain, how many molar equivalents of ATP could be
generated?
14. [REDACTED]

15. What is the material in Figure 4 (bottom of this page)?

16. What is the rate limiting enzyme of [REDACTED]?

17. What is the material on the right (Figure 1)?

18. What is the material on the right (Figure 2)?

19. What is the material on the right (Figure 3)?

20. The asterisk shows a radiolabeled carbon. [REDACTED]. What product will be radiolabeled?
21. Ammonium has a pKa of 9.25. [REDACTED] mL of [REDACTED] M HCl is added to [REDACTED] mL of a
[REDACTED] M buffer solution of this ammonium at pH [REDACTED]. The resulting solution is at pH:

22. In a test organism that possesses a normal TCA cycle, you determine that [REDACTED]. Which enzymatic failure
could best explain this observation?

23. What type of reaction does the [REDACTED] of TCA perform? (Hint: consider all products made, including side
products)

24. Which enzyme activity in TCA produces [REDACTED] product?

25. [REDACTED]

26. The asterisk shows a radiolabeled carbon, [REDACTED] where is this carbon found?

27. [REDACTED] can become what amino acid?

28. Which of the following amino acids is/are likely [REDACTED] at physiological pH? (Select all that apply)
29. Using glycolysis, given that [REDACTED] of glucose is converted to [REDACTED] of pyruvate, how many moles of
ATP are [REDACTED]? (Hint: Read this question carefully)

30. A mad-scientist wishes to make a glycolysis inhibitor to treat gerbilitis, why is this a bad idea? (Select all that
apply)

31. What does [REDACTED] protect against?

32. Which of the following glycolytic enzymatic reactions is [REDACTED] in cellular conditions?

33. What is the name of the enzyme that catalyzes the reaction of the correct answer to the previous question?

34. If [REDACTED] elevated in cells, what is the effect on the glycolysis pathway in those cells?

35. Which of the following structures is the product made by [REDACTED] glycolysis?
36. Which enzyme in glycolysis is the first one to produce [REDACTED]?

37. Dehydration of [REDACTED] produces:

38. Consider [REDACTED] inhibitor. What would be the changes expected in a Lineweaver-Burk Plot? (Select all that
apply)

39. During [REDACTED] conditions … (Select all that apply)

40. Fructose [REDACTED] in the cell, what would we expect?

41. Which of the following allosterically [REDACTED]?

42. How is [REDACTED] converted to [REDACTED]?

43. How is [REDACTED] converted to [REDACTED]?

44. Which of the following is a cause of [REDACTED]?

45. Does this span of amino acids possess a residue [REDACTED] used [REDACTED]: [REDACTED]
 Short Answer Question

PATHWAY TRACE

For this trace, we are going to trace an amino acid to a [REDACTED]

Starting with this amino acid: [REDACTED]

You must also NAME the amino acid! Use every box, do not add boxes,
do not remove boxes. Do not change clues. Ignore any shuttle steps.