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Australian Dental Journal

The official journal of the Australian Dental Association


Australian Dental Journal 2015; 60: 301–308

doi: 10.1111/adj.12230

Evaluation and prevention of enamel surface damage during


dental restorative procedures
T Milic,* R George,† LJ Walsh*
*School of Dentistry, The University of Queensland, Brisbane, Queensland.
†School of Dentistry and Oral Health, Griffith University, Gold Coast, Queensland.

ABSTRACT
Background: This study examined the effect of operator experience, dominance, tooth position and access, on frequency
and extent of iatrogenic damage to approximal tooth surfaces during conventional Class II cavity preparations. The
effectiveness of protective devices in minimizing damage was also explored.
Methods: 10 students and 10 experienced dentists each prepared 24 Class II cavity preparations in typodont teeth with-
out protection; 10 utilizing stainless steel matrix bands and 10 utilizing protective wedges. The frequency and extent of
damage were analysed with respect to the above variables. Subsequently, 20 natural and 20 typodont teeth were utilized
to establish the relationship in depth of damage caused by a high-speed diamond bur on typodont versus natural teeth.
Results: Dentists caused iatrogenic damage on 74% of approximal surfaces without protection, which fell to 50% and
46% respectively when matrix bands and wedges were used as guards. The corresponding rates of damage for students
were 94%, 80% and 44%. There was no difference in depth of damage caused on the two types of teeth when bur was
in contact with teeth for a very short time.
Conclusions: Greater operator experience and the use of guards reduces iatrogenic damage to proximal surfaces during
preparation with high-speed rotary instruments.
Keywords: Approximal surface, enamel damage, iatrogenic, protection.
Abbreviations and acronyms: DO = disto-occlusal; MO = mesio-occlusal.
(Accepted for publication 28 September 2014.)

wide, and also appears as fine scratches, indentations


INTRODUCTION
and extensive damage (a combination of defects over
Iatrogenic damage to approximal enamel during a large surface area).4,5 Such injuries can potentially
restorative procedures is an important clinical issue. It increase the risk of dental caries developing on the
has been reported that damage to adjacent teeth is affected surface by altering its properties.2,6–9
practically unavoidable during conventional full Removal of as little as 120 lm (200  80 lm) of
crown and Class II cavity preparations.1–3 Qvist et al. the outer layer of enamel results in an increase in
reported that primary teeth with extensive surface permeability to acid like, water-soluble molecules.6
damage require restorative treatment three times as As well, furrows and indentations retain dental pla-
often as undamaged surfaces within 3.5 years. They que in areas which are inaccessible to dental floss,
also reported that damaged permanent teeth required which increases the likelihood of caries initiation and
restorations four times as often as undamaged teeth progression.1,2,4,6,10 Furthermore, there is a concern
within five years.4 Taken together, results of in vitro that enamel damage on approximal surfaces will
and in vivo studies indicate that 64% to 97% of appear as interproximal radiolucencies which could
enamel surfaces of teeth adjacent to Class II cavity be misdiagnosed as carious cavitations, resulting in
preparations may be damaged.1,4 However, Medeiros unnecessary restorative treatment of non-carious
et al. reported a lower frequency of damage surfaces.2,5
(49–60%) and attributed this to elimination of small Despite their suitability, metal matrix bands are
defects over time.5 rarely used to prevent iatrogenic damage during Class
Iatrogenic damage to the enamel surface is most II preparations.1,5,11 The same is true for traditional
often in the form of vertical grooves up to 1 mm tooth separators.5,12 Recently, novel interproximal
© 2015 Australian Dental Association 301
T Milic et al.

separating wedges with a thin stainless steel plate The frequency and extent of enamel surface damage
have been developed that may be inserted with mini- for the protected surfaces of premolars and second
mal time and preparation.13 There are no studies on molars adjacent to the Class II preparations were
the effectiveness of use of these novel protective compared to those for the unprotected surfaces with
devices to minimize the extent of enamel iatrogenic regards to the following parameters: operator experi-
damage inflicted during preparation of approximal ence, operator dominance (preparations on operators’
surfaces.14 Therefore, the aim of this study was to dominant versus non-dominant side, i.e. 16 vs 26, 36
investigate the frequency and extent of iatrogenic vs 46), tooth position (maxillary vs mandibular), ease
damage during Class II preparations in contemporary of access (MO vs DO), use of guards and their type.
dental practice and compare the effectiveness of novel
and existing protective devices in preventing and mini-
Assessment of damage
mizing iatrogenic damage. Further, the study also ver-
ified that the assessment of these defects in plastic The surfaces of premolars and second molars adjacent
typodont teeth is a valid approximation to the clinical to Class II preparations were initially assessed using
situation. 3x magnification. Defects were classified by type
(scratches, indentations, grooves, multiple indenta-
tions and/or grooves, extensive damage), which is a
MATERIALS AND METHODS
slight modification of an earlier scheme by Medeiros
et al.5 As per Medeiros’ scheme, a groove was defined
Study design
as a defect deeper than a scratch, longer than it is
wide and extending vertically or horizontally. An
Part 1: Evaluation of iatrogenic damage with and
indentation is a defect where the length is similar to
without guards
the width. Scratches are shallow lines, often multiple
A total of 10 final year dental students and 10 experi- and commonly long. Multiple damage is a combina-
enced (>5 years since graduation) general dentists par- tion of grooves and/or indentations that do not spread
ticipated in the project. Ethical approval for the study over a large area. Extensive damage covers a large
was obtained from the University of Queensland proportion of the enamel surface, and it often consists
School of Dentistry Research Committee, Brisbane, of all the other types of damage.5
Australia. Width and length of surface defects were measured
A standardized cavity preparation method was using a calibrated digital micrometer to the nearest
used, and the written and verbal summary of the 0.01 mm (Mitutoyo Corporation, Kawasaki, Japan).
whole process was given to participants before they Two assessors analysed all samples independently.
consented to participate in the study. The environ- Where results differed by more than 10% the mea-
mental and clinical conditions were maintained the surement was repeated in duplicate and the average
same for all operators, i.e. identical lighting, phantom value of the results that were within 10% from each
heads; identical dental units using water cooling and other were taken. Further, 3X magnification was used
compressed air; handpieces (NSK Pana-Max PAX-TU for the categorization of teeth into four groups based
M4, Tochigi, Japan); medium grit cylindrical diamond on the estimated depth of damage (minimal, shallow,
high-speed burs (Horico Diamant FG108010, Horico, moderate and deep) for further analysis with high pre-
Berlin, Germany). cision instruments. Initially, the teeth with apparent
This part of the study utilized only plastic typodont minimal and maximal damage in each category were
teeth (Frasaco GmBH, Tettnang, Germany), which analysed using a non-contact 3D scanner (ATOS
were similar to those used by all operators during Gesellschaft f€ur Optische Messtechnik GR, GOM
their dental school preclinical training. All teeth were mbH, Braunschweig, Germany), which had a resolu-
from the same manufacturing batch, to eliminate the tion of 10 lm and an accuracy of ≤1 lm. As a result,
impact of any interbatch variability. the categories were assigned the following ranges
Sets of four typodont plastic premolars, together (minimal <30 lm; shallow from 30 to 60 lm; moder-
with adjacent first and second molars were consecu- ate from 60 to 120 lm; and deep ≥120 lm). All dam-
tively mounted in phantom heads. Each operator pre- age of 120 lm and beyond was classified as ‘deep’,
pared 24 Class II cavity preparations on first molars based on the finding of Kuhar et al. that damage to
(12 each of mesio-occlusal (MO) and disto-occlusal enamel surfaces to a depth ≥120 lm increases the per-
(DO) designs). Following this, each operator prepared meability to acid-like molecules.6
10 Class II preparations on first molars, utilizing Following this, teeth with damage that was assessed
either a FenderWedgeâ (Directa AB, Upplands V€asby, as shallow, moderate and deep with 3x magnification
Sweden) interproximally or metal matrix bands for were analysed using the non-contact 3D scanner. In
protection of adjacent teeth. addition, selected teeth from all categories including
302 © 2015 Australian Dental Association
Enamel surface damage

minimal were analysed to determine maximal depth Part 2: Investigation of the relationship between the
of defects either using a 3D visible light profilometer depth of damage caused on plastic and natural teeth
with vertical accuracy of 60 nm (Veeco Wyko NT under controlled conditions
9100, NY, USA) or a 3D visible laser profilometer
This part of the study used 20 freshly extracted
(Talysurf CCI, Taylor Hobson, Leicester, UK) of the
human teeth as well as 20 typodont teeth. All natural
same accuracy (Fig. 1). Samples of damaged teeth of
human teeth were extracted for orthodontic and other
each defect type (extensive, groove, indentation,
reasons. All teeth were stored at 4 °C and used within
scratch) were sputter coated with gold (2–5 nm) and
six months of extraction (in line with ISO/TS 11405).
examined using scanning electron microscopy (Zeiss
The inclusion criteria were absence of restorations,
Sigma Field Emission SEM with Gemini column, Carl
caries, cracks and visible signs of enamel deteriora-
Zeiss, Obercochen, Germany), to gain representative
tion. All natural teeth were kept fully hydrated and
images of various defects with a high depth of field
the time of exposure to air was limited to five minutes
(Fig. 2).
during every stage of the experiment.
To validate the visual test method used for categori-
An initial viability study was done using 10 plastic
zation of teeth, the results of 30 teeth assessed with
and 10 natural teeth under controlled load (force)
optical microscope were compared to results for depth
from an Instronâ universal testing machine (Model
measurements from the Veeco profilometer and the
5940 Single Column Testing System, Instron Corpora-
3D scanner on the same samples. The acceptance cri-
tion, Norwood, MA, USA). This viability study was
teria of <10% incorrectly categorized teeth was met
done using variable forces (0.2, 0.4, 0.6, 0.8 and 1N)
and the method was validated.
on a medium grit tapered diamond bur (MANI

X Profile
X: 222.2599 um
um
30
mm
1.2

20

Z: 47.3900 um
1.0
10
0.9

0.8 0

0.7
-10

0.6

-20 um
0.5 0 100 200 300 400 500

0.4

0.3
Y Profile
Y: 0.6824 mm
0.2 um
30
0.1
20
0.0 mm
10
0.00 0.10 0.20 0.30 0.40 0.57

-10
X 0.20 - - mm
Y 0.25 - - mm -20

Ht -15.92 - - um -30
Dist - mm
N/A

-40
Angle -
mm
0.00 0.20 0.40 0.60 0.80 1.00

Fig. 1 Depth measurement of 47 lm deep iatrogenic damage taken by the 3D visible light profilometer. The data readout shows the X profile in lm and
the Y profile in mm.

© 2015 Australian Dental Association 303


T Milic et al.

holders and ground to a flat surface under water


(AudioMet 3000, Buehler, Lake Bluff, Illinois, USA)
and polished progressively to a final grit size of
0.1 lm. The natural teeth remained hydrated during
this preparation. The microhardness (VHN) of the
second set of 10 natural teeth and 10 plastic teeth
was measured using a calibrated microhardness tester
(DuraScan, Struers A/S, Ballerup, Denmark) at a dwell
time of 20 seconds and a load of 25 g, to maintain
consistency with parameters reported in the literature.15

Statistical analysis
A power analysis of the first part of this study was
calculated for two groups of operators (students and
experienced dentists) and three levels of guard.
Assuming a power of 0.8, a type 1 error alpha of
Fig. 2 Damage to a natural tooth in the form of an indentation (A), a 0.05 and a sample size of over 400 teeth, this study
groove (B), and a scratch (C). This particular example is from the palatal
surface of an extracted natural tooth which had been mounted in an had sufficient power to detect an effect size as small
anatomical position along with other teeth in plaster blocks, as part of a as 0.015. Similarly, a power analysis of the second
pilot study. The types of defects illustrated on the palatal surface are part of this study (power of 0.8 and a sample size of
typical for the patterns of iatrogenic damage recorded in the study on
approximal enamel surfaces. over 100) was calculated for plastic and natural teeth
and six different forces (0.1 N, 0.2 N, 0.4 N, 0.6 N,
0.8 N, 1 N). Assuming a power of 0.8 and a sample
Dia-Burs TF-S20, Mani Inc., Tochigi, Japan). The bur size of over 100, this study had sufficient power to
was kept in contact with teeth for approximately detect an effect size of 0.1.
200 milliseconds. No water-cooling was utilized dur- Data were analysed in JMP statistical analysis soft-
ing drilling. ware (Version 10) using chi-squared tests and linear
Following this initial viability study, a final investi- regression. Damage was analysed both as a continu-
gation was undertaken with a further 10 natural and ous and a categorical variable. Chi-squared tests anal-
10 plastic teeth using an expanded range of forces of ysed the effect of operator experience, dominance,
0.1 N, 0.2 N, 0.4 N, 0.6 N, 0.8 N and 1 N. The bur- tooth position, access and use of a guard on damage
tooth contact time of 200 milliseconds was recorded as a categorical variable.
by the machine’s in-built timer and simulated the acci- A multifactorial ANOVA was used to test the rela-
dental slip of the bur clinically. The dental drill (NSK tionship between depth of drilling when varying forces
Pana-Max PAX-TU M4, Tochigi, Japan) was were applied on the drill on natural and plastic teeth.
mounted on a holder and attached to the load cell of Effect tests were performed on type, force and the
an Instronâ Universal testing machine (Model 4848 interaction between type and force to determine the
MicroTester, Instron Corporation, Norwood, MA, statistical significance of all three groups. Differences
USA) so as to maintain a constant force for each between the means were assessed using least squared
experimental load. The instrument was calibrated and means differences Tukey tests.
had an accuracy of 0.25% of the indicated load. The
bur type utilized was identical to that used in Part 1
RESULTS
(Horico Diamant FG108010) of this study. No water-
cooling was utilized for drilling with the Instron; how-
Demographic information
ever, the bur was immersed in water for a brief time
before each drilling to simulate wet cutting to some Of the operators in the study, 55% were males. All
extent. The teeth were analysed using the ATOS non- final year dental students were aged in their early twen-
contact 3D scanner for measurement of the depth of ties. While the age of the dentists was not recorded, all
defects, in a similar manner as Part 1 of this study. had five or more years of clinical experience.

Evaluation of microhardness of natural and plastic Part 1: Evaluation of iatrogenic damage with and
teeth without guards
The relative microhardness of natural and plastic teeth Initial analysis was conducted using visual scoring of
was compared. The teeth were fixed to resin sample all teeth from Part 1 at 3x magnification (Table 1).
304 © 2015 Australian Dental Association
Enamel surface damage

Table 1. Percentage of surfaces damaged by experi- Part 2: Relationship between the depth of damage
enced dentists (ED) and students (S) drilling without caused on plastic and natural teeth
the use of guards, by type of damage
For the initial viability study, a model for depth of
Type of damage % of surfaces % of surfaces damage was fitted utilizing a multifactorial ANOVA,
damaged by ED damaged by S examining the effect of tooth type, force used, and the
ND – No damage 25 6 interaction between these (R squared = 0.60, p <
S – Scratch 14 12 0.0001). Some 60% of the variation in depth is
I – Indentation 13 10
G – Groove 20 15
explained by the variation in type and force. Effect
M – Multiple 23 36 tests on type, force and the interaction between these
E – Extensive damage 5 21 determined significance of only force as a variable (p <
Total 100 100
0.0001), with no significant difference in the depth
achieved for drilling between natural and plastic teeth
at the stated contact time of (t ~ 200 ms). The least
squares mean differences Tukey test determined no sig-
Students caused both multiple and extensive damage nificant difference between natural and plastic at all
more frequently than dentists. levels of force; however, there were differences in the
With regard to variables affecting surface damage, depth as the force increased incrementally.
dominance, tooth position and access did not exert Following the success of this initial testing, the data
significant effects, while operator experience and use for the final investigation of drilling into plastic and
of a guard were significant and therefore included natural teeth with an expanded range of forces and an
in the final model (p = 0.0086 and p < 0.001). additional measurement of microhardness were analy-
Greater operator experience and use of a guard sed. Microhardness testing was performed twice on
independently reduced the extent of damage each tooth to control for any measurement errors,
(Table 2). The changes in the mean depths of dam- and the average was taken due to the high correlation
age as a function of utilizing guards are illustrated (Spearman’s Rho 0.617, p = 0.0038). The initial mul-
in Fig. 3. tifactorial ANOVA model included a control for
A linear regression model with defect volume microhardness, which did not significantly contribute
(calculated from area and depth) as a continuous var- to the model and so was excluded. The average
iable (R square 0.27, p < 0.0001) revealed that both microhardness for plastic teeth was 56.02 VHN and
greater operator experience and use of a guard, and for enamel 312.8 VHN.
the interaction between these two factors, were all A model for depth of damage from drilling was
significantly associated with a lower volume of dam- then fitted using multifactorial ANOVA, examining
age (p < 0.0001, p < 0.0001 and p = 0.0001 respec- the effect of type of tooth, force used, and the interac-
tively). The Tukey test demonstrated a significant tion between them (F = 83, p < 0.001). Some 89% of
difference in the volume of damage between students the variation in depth could be explained by the varia-
and experienced dentists when no guard was utilized tion in type and force. An effect test on force as a var-
(0.6 mm3, CI 0.33–0.79). There was also a significant iable was significant (p < 0.0001). Tooth type and the
difference for both groups with the use of guards, interaction between type and force were not signifi-
but no significant difference between the two guard cant (p > 0.05), indicating no statistically significant
types (Table 3). difference between natural and plastic teeth at all

Table 2. Percentage of surfaces damaged by experienced dentists (ED) and students (S) drilling with and without
guards, by depth of damage
Extent of damage Frequency of teeth damaged by operators
(depth in lm)
ED – No ED – Stainless ED – Wedge S – No S – Stainless S – Wedge (%)
guard (%) steel band (%) (%) guard (%) steel band (%)

N – No damage (ND) 25 50 54 6 20 56
D – ND < x < 30 11 15 16 5 20 10
D – 30 ≤ x < 60 5 16 24 8 9 10
D – 60 ≤ x < 120 10 6 1 11 14 6
D – 120 ≤ x < 200 20 5 5 26 23 6
D – 200 ≤ x < 300 17 4 0 27 4 8
D – ≥ 300 12 4 0 16 11 4
Total 100 100 100 99 101 100

© 2015 Australian Dental Association 305


T Milic et al.

250 250
Students
Experienced dentists 200
200

Mean Depth (µm)


150

150
100

100 50

0
50 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
Force (N)

Fig. 4 The effect of increasing force (in newtons) on depth of damage


0 (in micrometres).
No guard Steel band FenderWedge

Fig. 3 Variations in the mean depth of damage (in micrometres) accord-


ing to operator experience and guard type (no guard, stainless steel band
or integrated wedge and band). Bars show standard deviations. Class II cavity preparations.5 The authors considered
that direct supervision and the influence of assessment
and grading for undergraduate students could explain
Table 3. Least squares means table of volume of the observed low level of iatrogenic damage in their
damage caused by experienced dentists and students study. Students were not individually supervised nor
with and without guards graded in the present study. In addition, the difference
in the statistical power of the present study (480
Operator, guard Mean Lower 95% Upper 95%
volume confidence confidence preparations) compared to the 1999 investigation
(mm3) interval interval (35 preparations) may have contributed to a differ-
ence in the observed effect of operator experience.
ED, None 0.394 0.285 0.504
ED, Tofflemire 0.114 0.004 0.224 The present study found that without the use of
ED, Wedge 0.040 –0.069 0.150 proximal guards, students caused damage to an exten-
S, None 0.954 0.845 1.063 sive area of the tooth four times more often than
S, Tofflemire 0.281 0.172 0.391
S, Wedge 0.186 0.059 0.277 experienced dentists. Further, 69% of damage caused
by students was 120 lm or deeper, compared to 49%
for experienced dentists. As Kuhar et al. demonstrated
forces. Figure 4 illustrates mean depth of damage by that damage deeper than 120 lm increases the perme-
force. There was a significant difference between ability of enamel to acid-like molecules,6 this fre-
depth at forces of 1 N, 0.8 N, 0.6 N, 0.4 N and quency of occurrence of deep iatrogenic damage is
0.1 N, but not between 0.4 N and 0.2 N and between concerning.
0.2 N and 0.1 N forces. The present study examined the majority of vari-
ables that the literature identified as potentially con-
tributory to the occurrence and extent of iatrogenic
DISCUSSION
damage, but did not examine the influence of magni-
This study provides several insights into the factors fying loupes. Lussi et al.2 explored whether the use of
which affect the frequency and severity of damage to loupes could reduce iatrogenic damage in a compre-
approximal surfaces during tooth preparation. Given hensive study that involved at least six months of
that the necessary manual skills for dental clinical training of operators in the use of loupes. A signifi-
practice develop during dental school training and cant increase in the occurrence and extent of damage
improve with practice over time,16,17 it was expected to adjacent tooth surfaces during Class II cavity prep-
that experienced operators would cause less iatrogenic arations on first molars occurred when loupes were
damage than undergraduate students under the same utilized. The results were explained on the basis of
conditions. This prediction was confirmed in that den- further narrowing of the field of view already
tal students damaged 19% more adjacent surfaces restricted by indirect vision.
than experienced dentists. Similar results were Of the variables examined in the present study, use
reported previously in the literature with regard to iat- of a guard was found to reduce the depth and occur-
rogenic damage caused during full crown prepara- rence of iatrogenic damage, with both stainless steel
tions.10 However, a 1999 study by Medeiros et al. matrix bands and protective separating wedges being
reported contradictory results, in that experienced equally effective. Furthermore, as shown in Table 2,
operators caused almost three times more damage stainless steel matrix bands and wedges not only
(64%) than undergraduate students (23%) during prevent damage but minimize it when it occurs.
306 © 2015 Australian Dental Association
Enamel surface damage

Damage was confined to less than 60 lm for experi- does not impact on the validity of the study as the
enced dentists in 81% of cases utilizing stainless steel second part of the project demonstrated that iatro-
bands, and in 94% of cases where wedges were uti- genic damage inflicted on typodont teeth under condi-
lized. For students, the damage was contained below tions that simulated accidental slippage of the bur
60 lm in 49% of cases and 76% of cases utilizing was representative of the damage that would be
stainless steel bands and wedges respectively. This inflicted on natural teeth during the same procedures.
finding is considered particularly important in terms A second, albeit minor, limitation relates to the fact
of mitigating the potential consequences of iatrogenic that the use of the Instronâ was only possible in dry
damage. conditions. There are very few studies on the effect of
The present study did not find any significant differ- dry/wet cutting on extracted natural or plastic teeth
ence in the benefits provided between protective with diamond burs.18 Immersing the bur in water for
wedges with a stainless steel plate and ordinary stain- a short period of time prior to drilling could not fully
less steel matrix bands. Nevertheless, operators in the replicate wet cutting conditions. However, Elias et al.
study described the interproximal wedges as easier reported that there was no statistically significant dif-
and faster to place. The operators commented that ference in the cutting forces required during wet ver-
they could tend to rely too much on protection affor- sus dry cutting of Class II cavity preparations in
ded by the stainless steel component and by more natural teeth using carbide tungsten burs.18
aggressive drilling or less attention to fine manual Future studies in this area may investigate any justi-
control may inadvertently have caused deeper iatro- fiable reasons for the previously observed lack of
genic damage by drilling through the steel. This would operator utilization of protective devices, as well as
explain the finding that damage over 200 lm deep examining suitable materials for protection of iatro-
occurred for students in 15% and 12% of adjacent genically damaged surfaces in lieu of classical
teeth when wedges and stainless steel bands were used restorative work.
respectively, compared to the corresponding 0% and
8% for experienced dentists. CONCLUSIONS
Overall, this study shows that the benefit of protec-
tive systems outweighs the risk of occasional deep iat- As both novel and traditional protective devices can
rogenic damage. Therefore, it is recommended that a prevent iatrogenic damage and minimize its conse-
protective device be used when more difficult cavity quences, there is a clear logic in their wider use, par-
preparations are being undertaken in clinical practice, ticularly in teaching clinics and preclinical instruction.
e.g. molar Class II cavity preparations or crown prep- The results of the study also stress the need to raise
arations in situations where there are complicating awareness of the potential for iatrogenic damage and
factors such as more difficult patients, poor access, or to remind clinicians of its consequences.
restricted vision. It is further recommended that sepa-
rating wedges or protective matrix bands be used reg- ACKNOWLEDGEMENTS
ularly by students in preclinical training, as well as by
graduates in clinical practice. However, the use of This study was supported by a research grant from
protective devices does not obviate the need for care the Australian Dental Research Foundation and the
while drilling. 2013 Colin Cormie Scholarship. The authors thank
The results of the second part of the study showed no all operators for their participation. We also thank
significant difference in the extent of damage caused Mr Nic Croce and Ms Megan Valentine from the
during brief contact (t ~ 200 ms) of a high-speed med- Statistical Consulting Unit (University of Newcastle),
ium grit rotary diamond bur with plastic and natural Ms Amanda Cosgriff and Mr Tony Clayton of SDI
teeth at any of the force levels (0.1 N – 1 N). This indi- Ltd, Mr Dennis Balacano and Mr Matt Smith of
cates that results obtained for damage to Frasaco plas- Cochlear Ltd, Mr Kevin Warwick of ScanXpress, and
tic teeth can be extrapolated to natural teeth. Mr Khu Vu of the ANU RSPE for their assistance in
Despite all attempts to replicate in vivo conditions, using materials testing facilities and equipment.
several limitations of the present study must be recog-
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