The effects of Tarantula cubensis venom on

open wound healing in rats

N.Y. Gul Satar
, 
I.T. Cangul
, 
A. Topal
, 
H. Kurt
, 
V. Ipek
, 
G.I. Onel
Published Online: 9 Feb 2017 https://doi.org/10.12968/jowc.2017.26.2.66

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Abstract
Objective:
The aim of this study was to evaluate the clinical and histopathological effects of two
different dosages of alcohol extract of Tarantula cubensis (Theranekron) on open wounds.

Method:
A total of 24 Sprague-Dawley rats were randomly divided into Tarantula cubensis extract
(TCE1, n=8) 1/10 diluted, TCE (TCE2, n=8), and (3) vehicle-control (0.2 ml of 96 %
ethanol, n=8) groups. Experimental full-thickness 1 x 1cm wounds were created on dorsum
skin. TCE or vehicle were given systemically by subcutaneous injections on postoperative
days 1 and 4. Wound planimetry and procurement of biopsies was performed on days 4, 8,
12 and 16.

Results:
The mean non-epithelialised wound area in the vehicle-control group was significantly
larger than in the TCE1 group on days 4, 8, 12 and 16, and in the TCE2 group on days 8, 12
and 16 (p<0.05). The mean percentage of wound contraction was significantly higher in the
TCE1 and TCE2 groups than in the vehicle control group on days 8, 12 and 16 (p<0.05).
Histopathologically, wound healing was characterised by a significant decrease in the
 neutrophil counts and a significant increase in neovascularisation; neither were effected by
TCE.

Conclusion:
Our results suggest that alcohol extract of Tarantula cubensis accelerates epithelialisation
and, thus, has beneficial effects on open wound healing in rats.

https://www.magonlinelibrary.com/doi/pdf/10.12968/jowc.2017.26.2.66

Effects of Tarantula Cubensis D6 on

Aflatoxin-Induced Injury in
Biochemical Parameters in Rats
Mürsel Karabacak 1, Gökhan Eraslan 2, Murat Kanbur 3, Zeynep Soyer Sarıca 4
Affiliations expand

 PMID: 26143454

 DOI: 10.1016/j.homp.2015.02.005

Abstract
Introduction: Aflatoxins are toxic fungal metabolites that have adverse effects on
humans and animals. Tarantula cubensis D6 is used as a homeopathic medicine for
different purposes. The present study investigates the effects of Tarantula cubensis
D6 on the oxidant-antioxidant balance and some biochemical parameters against
exposure to aflatoxin.

Methods: Thirty-two Sprague-Dawley female rats were used and evenly divided

into four groups. Group 1 served as control. Groups 2, 3, and 4 received 200
μl/kg.bw/day Tarantula cubensis D6 (applied subcutaneously), 400 μg/kg.bw/day
total aflatoxin (approximately 80% AF B1, 10% AF B2, 6 %AF G1, and 4% AF G2),
and 200 μl/kg.bw/day Tarantula cubensis D6 plus 400 μg/kg.bw/day total aflatoxin,
respectively, for 28 days. At the end of 28 days, blood samples and some organs
(liver, kidney, brain, and spleen) were taken from all the animals. Oxidative stress
markers (MDA, SOD, CAT, GSH-Px) and some biochemical parameters (glucose,
triglyceride, cholesterol, BUN, creatinine, AST, ALT and ALP, total protein, albumin)
were evaluated in blood samples and tissues.

Results: Aflatoxin caused negative changes in all oxidative stress parameters and

some biochemical parameters (glucose, triglyceride, cholesterol, creatinine, AST,
ALT, ALP, total protein, albumin). Administration of Tarantula cubensis D6 partly
alleviated aflatoxin-induced negative changes.

Conclusions: Our results indicated that Tarantula cubensis D6 partially neutralized

the deleterious effects of aflatoxin.

Keywords: Aflatoxin; Biochemical parameters; Oxidative stress; Rat; Tarantula

cubensis D6.

Copyright © 2015 The Faculty of Homeopathy. Published by Elsevier Ltd. All rights
reserved.

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MeSH terms
 Aflatoxins / antagonists & inhibitors*
o
o
o
 Aflatoxins / toxicity
o
o
o
 Animals
o
o
o
 Antioxidants / pharmacology
o
o
o
 Antioxidants / therapeutic use*
o
o
o
 Female
o
o
o
 Oxidative Stress / drug effects*
o
o
o
 Rats
o
o
o
 Rats, Sprague-Dawley
o
o
o
 Spider Venoms / pharmacology
o
o
o
 Spider Venoms / therapeutic use*
o
o
o

Substances
 Aflatoxins
 Antioxidants
 Spider Venoms

https://pubmed.ncbi.nlm.nih.gov/26143454/