Ifrdnbt Abstract Book

International Conference on Innovations and Future Research Dimensions on Nanobio Pharmaceutical Technology
INTERNATIONAL CONFERENCE ON
INNOVATIONS AND
FUTURE RESEARCH DIMENSIONS ON
NANOBIO PHARMACEUTICAL TECHNOLOGY
7-8th October, 2014
BOOK OF ABSTRACTS
ORGANIZED BY
DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY
CENTRE FOR EXCELLENCE IN NANOBIO TRANSLATIONAL RESEARCH
BHARATHIDASAN
Organised by: Dept. of Pharma. Tech., & CentreINSTITUTE
for Excellence in OF TECHNOLOGY
Nanobio CAMPUS
Translational Research,
Anna University, BIT Campus, Tiruchirappalli - 620024
TIRUHCIRAPPALLI – 620024, TAMIL NADU, INDIA
VISION AND MISSION
ANNA UNIVERISTY
BHARATHIDASAN INSTITUTE OF TECHNOLOGY CAMPUS
TIRUCHIRAPPALLI – 620024 TAMIL NADU
DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY

CENTRE FOR EXCELLENCE IN NANOBIO TRANSLATIONAL RESEARCH
****************************************
VISION
To provide quality education, foster research and development in
Pharmaceutical Technology, evolve innovative solutions for industrial
problems and serve for humanity, upraise the department to reach the
apex of glory in pharma education and research to be cognizant of as
Centre for Excellence.
MISSION
Deeply inoculate the treasure of pharmacy education and research into

the inquisitorial minds of students through practicing with state of the
art infrastructural facilities to instigate the multi - disciplinary expertise
to meet the needs of pharmaceutical and allied industries.
Provide a dynamic educational experience, create outreach

opportunities and empower them to become leaders of profession
Advocate the responsibility to contribute for the improvement in public

health by developing and translating concepts into practice within the
state and beyond.
MESSAGES
ANNA UNIVERSITY
TIRUCHIRAPPALLI - 620 024
Dr.T.SENTHIL KUMAR
Dean, BIT Campus
MESSAGE
The Anna University welcomes all delegates and participants of the DBT & TEQIP II sponsored
International Conference on Innovations and Future Research Dimensions on Nanobio Pharmaceutical
Technology (IFRDNBT). Anna University, a largest affiliating unitary type of University in 1978 and
presently offers higher education in Engineering, Technology and allied Sciences relevant to the
current and projected needs of the society as an affiliating Technical University in the state of Tamil
Nadu with its campuses in Tiruchirappalli, Coimbatore, Tirunelveli and Madurai.
We are stepping ahead to become a world-class Technical University and a University of first
choice in India by 2023 as per the vision 2023 statement of Government of Tamil Nadu to become the
knowledge capital and innovation hub in India. We provide education, research, and high quality
consultancy services in engineering and technology that cultivate creativity and innovation; and
therefore, committed to be in the forefront of engineering and technology. Besides teaching and
research, dissemination of knowledge gained therefrom to other associated community is a routine
affair of our University.
Nano and Biotechnology has widespread applications in the drug discovery, development,
delivery with many other promising clinical applications. Significant number of research reports is
being made available recently. Propagation of such knowledge and technologies in the field of nano
and biotechnology for societal and healthcare needs of is of prime priority.
I am confident that this International Conference will review the state of the art and recent
trends in the above technologies for further advancement.
I wish you all an amazing experience in sharing and exchanging ideas and knowledge. I also
congratulate the members of Organizing Committee from the Department of Pharmaceutical
Technology, BIT Campus, Anna University. I also thank the sponsors especially DBT, New Delhi again
for extending support of our noble endeavors!
Best wishes,
(Dr.T.SENTHIL KUMAR)
ANNA UNIVERSITY
Dr.K.RUCKMANI
Professor and Head, Dept. of Pharma. Tech.,
Director, Centre for Excellence in Nanobio Translational Research
MESSAGE
We want to thank everyone in attendance for convening in Anna University, BIT campus at The
International Conference of Innovations and Future Research Dimensions on Nanobio Pharmaceutical
Technology, to bring together the top nanotechnologists in the world. We are elated to bring together
invited speakers and more than 150 participants from all-around India to disseminate their knowledge
involving nanotechnology and its applications. This meeting aims to provide a platform for researchers from
academia, government, and the pharmaceutical industry to share existing knowledge, vision, technology,
and challenges in the field and promote collaborations among researchers interested in advancing this
fascinating scientific discipline.
We hope to provide insight into many of the valuable applications that nanotechnology has to offer
to the scientific community.
I congratulate the coordinators for their tireless efforts to make this event memorable. My very
best wishes for the conference to be successful and very useful for everyone associated with it.
ANNA UNIVERSITY
MESSAGE
We would like to personally welcome each of you to the TEQIP II and Department of Biotechnology Sponsored
International Conference on Innovations and Future Research Dimensions on Nanobio Pharmaceutical Technology organized
by the Department of Pharmaceutical Technology scheduled during 7-8th October, 2014.
It’s an exciting time for Department of Pharmaceutical Technology, Bharathidasan Institute of Technology Campus
of Anna University as we continue to grow, always adaptable, motivated and responsive to the rapidly changing academic
and research requirements in order to meet the global standards through all possible curricular and co-curricular activities.
Recently our department has been recognized as a Centre for Excellence in Nanobio Translational REsearch (CENTRE) by
the syndicate of Anna University and has created a huge impetus and drive to be more and more responsible in dissemination
of knowledge further fueled by TEQIP and central grants from DST, DRDO, UGC, BRNS & TNSCST for research project
proposals, workshops and seminars.
The world of Nanobio Pharmaceutical Technology is an exciting area in which to learn and to do research studies
etc., and we’ll continue to meet and bring inspired people together in forums like this, to ensure our institution remains
at the cutting edge. We’re transforming the way we operate to continuously improve our ability to translate the nanobio
pharmaceutical technology based research findings in to purposeful products for societal health care needs. Our students
and scholars have continued to meet the challenges of our field and to excel despite setbacks. We are very much proud of
where we are today and excited about where we are headed.
We would like to give you an idea of what you can expect and what we hope to achieve over the next two days.
Firstly, we are honored to have with us as our Chief Guest of today’s event Dr.R.Jayavel, Professor and Director, Centre
for Nanoscience and Technology, Anna University, Chennai, our keynote speakers, Sudeshna Chandra, Visiting Professor,
Technische Universität Chemnitz, Institut für Chemie Lehrstuhl für Anorganische Chemie, Germany, Dr.Vanaja K, Visiting
Scientist, Eberhard Karls Universitat, Tuebingen, Germany, Dr. Sivakumar Gowder, College of Applied Medical Sciences,
Qassim University, KSA and Dr.Gauthaman Karunakaran, College of Pharmacy, College of Medicine, King Khalid University,
Abha, KSA. With their long list of achievements, everybody will be so eager to hear the secrets of their successful research
results and experience gathered in their professional way. They are the people who would be spearheading nanotechnology
research in near future. Apart from the above there are a handsome of research results to be presented by fellow delegates.
The full length research articles submitted by the delegates have been published as Book published by ELSEVIER and Book
of Abstracts in digital format.
Throughout this conference, we ask you to stay engaged, keep us proactive and help us to realize the goals of this
conference and to shape the future of Nanobio Pharmaceutical Technology. Before we close, we would like to thank each
of you for attending our conference and bringing your expertise to our gathering. Our personal respect and thanks goes out
to all of you.
We sincerely thank all the authorities of Anna University for their valuable support and all the sponsors for their
wherewithal for the successful conduct of this conference.
(Dr.S.Latha & Dr.P.Selvamani)

ORGANISING COMMITTEE
ORGANISING COMMITTEE
CHIEF PATRON Dr.M.Rajaram
Hon’ble Vice Chancellor,
Anna University, Chennai
PATRON Dr.S.Ganesan
Registrar, AU, Chennai
CHAIR Dr.T.Senthil Kumar

Dean, BIT Campus
ADVISOR Dr.K.Ruckmani
Prof & Head
Dept. of Pharm. Tech., AU-BIT
COORDINATOR Dr.S.Latha
Assistant Professor
Dr.P.Selvamani
Assistant Professor
MEMBERS Dr.A.Puratchikody
Professor
Dr.N.Subramanian
Assistant Professor
Dr.E.Sanmugapriya
Assistant Professor
Dr.R.Vijaya
Assistant Professor
Mr.A.Shanmugarathinam
Assistant Professor
EDITORIAL BOARD CHAIRPERSON
Mrs.A.Umamaheswari
Assistant Professor
Mr.R.Suriyakanth
Assistant Professor
Dr.P.SenthamilSelvan
Assistant Professor
Mrs.K.Akilandeshwari
Assistant Professor
Dr.K.Kavitha
Assistant Professor
Dr.S.Lakshmanaprabu
Assistant Professor
Mrs.K.Vijayalakshmi
Assistant Professor
EDITORIAL Mr.C.Prabu
BOARD Research Scholar
MEMBERS Dept. of Pharm. Tech., AU-BIT
Mrs.M.ArputhaBibiana
Research Scholar
Ms.P.S. Dhivya
Research Scholar
Mr.S.Rajkumar
Research Scholar
Mrs.M.Poornima
Research Scholar
C.SHERLINA DAPHNY M.Tech., Final Year
S.MONISHA M.Tech., Final Year

T.SUPASSRI M.Tech., Final Year

INTERNATIONAL ADVISORY BOARD MEMBERS
SILVIO DUTZ Technische Universität Ilmenau, Germany
TANAJI T. TALELE St. John’s University, USA
JAGAT KANWAR Deakin University, Australia
MANIKAM SIVAKUMAR The Nottingham University, Malaysia
R.THIRUMURUGAN International Medical University, Malaysia
R. RAJAN International Medical University, Malaysia
L. MANIKANDAN Forma Therapeutics, Singapore
RENGANATHAN ARUN University of Zurich, Switzerland
ASHOK KUMAR ASIA Metropolitan University, Malaysia.
RAVI PALANIAPPAN Mercer University, Atlanta, USA
JITKANG LIM Universiti Sains Malaysia, Malaysia
KARINA POMBO GARCIA Helmholtz Zentrum Dresden Rossendorf, Germany
SHEEBA DAVID International Medical University, Malaysia.
DEEPAK BALAJI Thimiri Consulting Group, France
NIRMESH JAIN University of Sydney, Australia
VASUDEVAN MANI Universiti Teknologi MARA, Malaysia

NATIONAL ADVISORY BOARD MEMBERS
R. MANAVALAN Annamalai University, Tamil Nadu, India
RAJIV DAHIYA Global College of Pharmacy, UP, India
T.K. PAL Bioequivalence Study Centre, West Bengal, India
DHIRENDER BAGADHUR IIT, Mumbai
P.JAISANKAR Indian Institute of Chemical Biology, West Bengal,

India
J.K. GUPTA Jadavpur University, West Bengal, India
P. GAUTAM Anna University, Tamil Nadu, India
M. KRISHNAN Bharathidasan University, Tamil Nadu, India
P. RAJAGURU Anna University, Tamil Nadu, India
G.P. MOHANTA Annamalai University, Tamil Nadu, India
S. KAVIMANI Mother Teresa Post Graduate and Research

Institute, Pondicherry, India
C. DHANAPAL Annamalai University, Tamil Nadu, India
M.V. RAO Bharathidasan University, Tamil Nadu, India
T.N.K. SURIYA PRAKASH Al-Shifa College of Pharmacy, Kerala, India
P. GOPINATH IIT, Roorkee
RANU DUTTA University of Allahabad, Uttar Pradesh, India
L.K. GHOSH Jadavpur University, West Bengal, India
B.S. LAKSHMI Anna University, Tamil Nadu, India
G. MATHAN Bharathidasan University, Tamil Nadu, India
R. SIVAKUMAR Grace College of Pharmacy, Kerala, India
G. MARIAPPAN Kamla Nehru Institute of Management and

Technology, Uttar Pradesh, India
R. NETHAJI Devakiammal College of Pharmacy, Kerala, India
R.S. JEYA PRAKASH Manipal College of Pharmaceutical Sciences,

Karnataka, India
M. SIVAKUMAR Anna University, Tamil Nadu, India
M.B. VISWANATHAN Bharathidasan University, Tamil Nadu, India
T.K. RAVI Sri Ramakrishna Institute of Paramedical Sciences,

Tamil Nadu, India
J. NIRMAL Dr.HarisinghGour University, Madhya Pradesh,
India
D.C. SUNDARAVELAN Sri Ramakrishna Institute of Paramedical Sciences,
Tamil Nadu, India
K. NEHRU Anna University, Tamil Nadu, India
V. RAJESHKANNAN Bharathidasan University, Tamil Nadu, India
K. ASOKKUMAR Sri Ramakrishna Institute of Paramedical Sciences,

Tamil Nadu, India
C. VIJAYARAGHAVAN PSG College of Pharmacy, Tamil Nadu, India
ABSTRACTS
TABLE OF CONTENTS
NANOMATERIALS (ORAL)
CODE TITLE / AUTHOR PAGE NO
NM-OP-01 Synergistic antibacterial efficacy of two drugs in addition with 1

biosynthesized AgNPs from an allergenic fungus
N.Chitra
NM-OP-02 Green synthesis and characterization of herbal mediated silver nano 2

particles using Gymnema sylvestre (Linn.)
Kalakotla Shanker
NM-OP-03 Bio-toxicity of silver nanoparticles against multidrug resistant 3

pathogens and human epidermoid larynx carcinoma (HEP-2) cells
M.Lakshmipathy
NM-OP-04 Biosynthesis of silver nanoparticles using cloves of Allium sativum and 4

its antimicrobial activity
M.Abirami
NM-OP-05 Physiochemical properties of nanocomposite film made of chitosan 5

and Poly-L-lactic acid using PEG as plasticizer
B.Surumi
NM-OP-06 Larvicidal and antimicrobial potential of silver nanoparticles from a 6

potent actinomycete, Saccharopolyspora Erythraea
S.B.Prabha
NM-OP-07 Formulation and evaluation of mouth dissolving film leflunomide for 7

rheumatoid arthritis
A.Bhuvaneshwari
NM-OP-08 Antimicrobial, antioxidant and angiogenic potential of silver 8

nanoparticles from leaves of Murraya paniculata (l.) Jack
P.Rama
Organised by: Dept. of Pharma. Tech., & Centre for Excellence in Nanobio Translational Research,
NM-OP-09 Antioxidant and hemolytic property of silver nanoparticles synthesized 9

using Allium sativum
Neethu Hari
NM-OP-10 Tinidazole loaded nanoparticles for treatment of periodontitis: 10

formulation, optimization and characterization
M.Mahipal Y Rajput
NM-OP-11 Development of semisolid drug carriers for topical application based on 11

Solid lipid nanoparticles
Prajakta sarode
NM-OP-12 Improvement of physicochemical properties and antiangiogenic 12

efficacy of actarit by rapid dissolving nanoemulsifying technique
Shraddha hidadugi
NM-OP-13 In situ nanoemulsifying biaoactive phytoconsituent Chrysin for 13

improved physiochemical characteristics and in-vitro cytotoxicity
Deepali Govind Divate
NM-OP-14 Antibacterial potential of zinc oxide nanoparticles synthesized using 14

wet chemical method
T.Sudhakar
NM-OP-15 Green sonochemical synthesis of silver nanoparticles and their 15

applications
H. Joy Prabu
NM-OP-16 Production and characterization of fungal asparaginase bound silver 16

nanocomposite - a potent nanodrug for lymphocytic leukemia
Garrick Bikku George
NM-OP-17 Formulation and in vitro - in vivo evaluation of wedelo lactone loaded 17

nanosuspension for hepatoprotective activity in ccl4 induced
significant hepatic damage and oxidative stress model
P.Sucharitha
NM-OP-18 Antidiabetic activity of chromium (iii) nanoparticle against 18

Streptozotocin-induced diabetis in rats
Kanakalakshmi Annamalai
NM-OP-19 In-vitro cytotoxicity assessment of silver nanoparticles synthesized 19

using Aspergillus terreus against breast cancer cell line
M.D.Bala kumaran
NM-OP-20 A Novel Fenugreek Derived Vicenin-2 Gold Nanoparticles and Its 20

Glucose Uptake Effect in 3T3-L1 Adipocytes
P. Rajasekar and Shivashri
NM-OP-21 Phytogenic synthesis of silver nanoparticles and its potential for on blue 21
dye decolorization
P. Balashanmugam
NM-OP-22 Entrapment of Scoparia dulcis extract in chitosan alginate 22

nanoparticles for development of antimicrobial medical textiles
V.Rajalakshmi
NM-OP-23 Characteristic formulation of solid lipid nano particles encapsulated 23

thymoquinone and its approach on in vivo pharmokinetics and
biodistribution
T.Sumathi
NM-OP-24 Synthesis and characterization of Andrographis paniculata loaded nano 24

particles for the development of anti-microbial poly cotton fabrics
Hemalatha
NM-OP-25 Catalytic and biological activities of green silver nano particles 25

synthesized from Plumeria alba (Frangipani) flower extract
M.Rani
NM-OP-26 Biogenic silver nano particles from Ficus Religiosa: biological 26

application
N.Jayachandra Reddy
NM-OP-27 Preparation and characterization of solid lipid nanoparticles for 27

cerebral malaria
Girdhari Roy
NANOMATERIALS (POSTER)
NM-PP-01 Cost-effective microfluidic channels fabrication using soft lithography 28

Y.V Subba Rao
NM-PP-02 Design and evaluation of polyvinyl-gum ghatti self-assembled 29

nanoscale particles for oral delivery of simvastatin
Bibek Laha
NM-PP-03 In-vitro antimicrobial efficacy of zinc doped nanohydroxyapatite 30

against human pathogens
R.Baskar
NM-PP-04 Absorbance, transmittance and bandgap studies of complexing agent 31

triethanaolamaine added iron doped zinc sulfide thin films by SILAR
method
V. Helen
NM-PP-05 Pharmacokinetic study of nelfinavir nanocrystals. 32

Yogendra nayak
NM-PP-06 Formulation of olmesartan medoxomil nanosuspension: in-vitro 33

characterization and ex-vivo permeation
Rahul kumar Gunjal
NM-PP-07 Formulation, optimization and characterization of satranidazole loaded 34

PLGA nanoparticles
Singh Kanwar Sumran
NM-PP-08 Eudragit® RL 100 loaded ofloxacin nanoparticles: formulation by design 35

D.Nagane
NM-PP-09 Diclofenac sodium nanosuspension: an approach to improve anti- 36

inflammatory therapy
S. Aravindan
NM-PP-10 Redox environment cleavable polymeric nanoparticles for drug delivery 37

S.Angeline tisha
NM-PP-11 Syntheses and characterization of PAMAM dendrimer and its 38

application for removal of heavy metals
E.Gomathi
NM-PP-12 In-vitro cytotoxicity studies of nickel oxide nanoparticles on cancer cells 39

using MTT assay
K.Perumal Raj
NM-PP-13 Tin oxide nanoparticle catalyzed one-pot synthesis of 3, 4 40

dihydropyrimidinones
P.Sivakarthik, V.Thangaraj
NM-PP-14 Enhanced oral bioavailability of naringenin using polymeric 41

nanoparticles: formulation, characterization and in-vivo studies
P.Susheela
NM-PP-15 Synthesis of GSH & FA conjugated chitosan functionalized gold 42

nanospheres: physicochemical properties and applications in cancer
therapy
H.Kalpana
NM-PP-16 Green synthesis of silver nanoparticles from leaf extracts of breynia 43

vitis-idaea
T.P. Rajesh
NM-PP-17 Preparation and characterization of plumbagin loaded bovine serum 44

albumin nanoparticles for drug delivery applications
V.Vengateshwari and Bhuvana meenal
NM-PP-18 Biomimetic Production and cytotoxic effect of silver nanoparticles from 45

fungi
S.Akila
NM-PP-19 Development of anti-microbial diapers and wipes using the 46

combinatorial herbal extract loaded nanoparticles coated on the
plasma pretreated bamboo fabrics
K.Kongarasi
NM-PP-20 Synthesis and characterization of Andrographis paniculata loaded 47

nanoparticles for the development of antimicrobial poly cotton fabrics
R.Tharani
NM-PP-21 Effect of Complexing Agent Tea: The Structural, Morphological, 48

Topographical and Optical Properties of FexSx Nano Thin Films
Deposited By Silar Technique
Manikanden
NM-PP-22 Characterization studies of sunlight induced biosynthesis of silver nano 49

particles using solanaum melongena (egg plant)
Aravindan
NM-PP-23 Formulation of soap and cream from biosynthesised silver 50

nanoparticles of adathoda vasica nees. Leaf extract
H.Lindha Jeeva Kumari
NM-PP-24 Optimization studies on bioinspired green synthesis of silver 51

nanoparticles using clitoria ternatea
G. Swathi
NM-PP-25 Thermal, anti-fungal and primary electrochemical studies of palladium 52

nanoparticles
D. Prathyusha
NM-PP-26 Synthesis and characterization of michelia champaca leaf extract 53

loaded nanoparticles for the development of antimicrobial textiles
Sukumar Danpat
NM-PP-27 Synthesis of metaloxide nano composites for solar cells 54

S.Shanthi
NM-PP-28 Comparative analysis of secondary metabolites in leaves of Celery 55

(apium graveolens): a study on stability of celery reduced nano
particles.
R.Harish and M.Anbarasan
NM-PP-29 Influence of molar concentration on optical and morphological 56

characterization of EDTA added Tin sulphate (SnS) nano films by SILAR
method
Mani
BIOTECHNOLOGY (ORAL)
BT-OP-01 Qualitative screening of bioactive compounds of Artemisia nilagirica 57

(Clarke) pamp.
P.Parameswari
BT-OP-02 Hla g 14bp Indel polymorphism implicated in genetic predisposition to 58

asthma in Kodaikanal
V.J.Kavitha
BT-OP-03 Effective treatment of dyeing effluent using crab shell - chitosan as a 59

natural coagulant
C.Thamaraiselvi and Akhila
BT-OP-04 Isolation and screening of biosurfactant producing marine 60

actinomycetes
Mobeen Shaik
BT-OP-05 Optimization of media for the production of keratinase enzyme 61

T.P.Rajesh
BT-OP-06 Bioremediation of sugar wash using natural scavengers 62

A.Nithya
BT-OP-07 Packed bed column adsorption of cr (vi) onto acid treated Codium 63
tomentosum biomass
P. Suresh Babu
BT-OP-08 Colorimetric detection of S.aureus using peptide nucleic acid and 64

colloidal gold nanoparticles
M.Ananda Chitra
BIOTECHNOLOGY (POSTER)
BT-PP-01 Biological control of damping off and stem rot of tomato (Lycopersicon 65
esculentum Mill.) using an antagonistic actinomycete,
Saccharopolyspora erythraea.
S.B.Prabha
BT-PP-02 In-vitro and in-vivo bioactivity screening of alpha asarone 66

S.Asha Devi
BT-PP-03 Biotransformation of clozapine to norclozapine an active metabolite by 67

Cunninghamella elegans
S.Varalaxmi
BT-PP-04 Synthesis and microbial activities of some chalcones 68

V.Usha
BT-PP-05 A special emphasis on mitochondrial DNA in breast cancer therapy 69

T. Keerthana
BT-PP-06 Enhancement of Post-Harvest and Shelf Life of Carica papaya using 70

Aloe vera Gel Based Coatings.
A.Nandakumar, J. Ebenezer Samuel King, R. Arul Vel
BT-PP-07 Development of a smart device for detection of microbial bioflim 71

formation.
A.V.Ajitha Bharathi
PHARMACEUTICAL TECHNOLOGY (ORAL)
PT-OP-01 Preliminary phytochemical analysis and antifungal activity of 72

methanolic extract of leaves of Actinodaphne bourdillonii gamble
S. Deepa
PT-OP-02 Antimicrobial activity study of flavonoids and salicylic acid extracted 73

from Tagetes erecta Linn
R. Devika
PT-OP-03 New parameters in assessing cardiovascular risk in both non diabetic 74

and diabetic hyperlipidemia subjects: a case control study
Pusapati Madan Ranjit
PT-OP-04 Effect of nicorandil on the pharmacodynamics of metformin in animal 75

models
Suresh Goli
PT-OP-05 Determination of bioactive constituents of leaves of Corchorus 76

aestuans (l.) by GC - MS analysis
R.Dhanalakshmi
PT-OP-06 Molecular docking studies of ceftriaxone sodium with apoptosis 77

protein in colorectal cancer
P. Manimekalai
PT-OP-07 In-vitro antioxidant activities, total flavonoids and total phenolic 78

content of ethanolic extract from whole plant of lactuca runcinata (dc).
J. Amutha Iswarya Devi
PT-OP-08 Therapeutic potential of Pleurotus ostreatus: an edible mushroom in 79

human cancer cell line (MCF-7) and in rat mammary carcinoma
K.Deepalakshmi
PT-OP-09 A calcium channel blocker - amlodipine attenuates acetic acid induced 80

ulcerative colitis in mice
B.Rajinikanth
PT-OP-10 A single chemical entity to balance anti-amyloid and anti- 81

cholinesterase capacity: in silico drug design
Pavadai Parasuraman
PT-OP-11 Antioxidant activity and mosquitocidal activity of Allium sativum of 82

Kodaikanal hilly areas
M.Razia
PT-OP-12 Invitro antioxidant and hepatoprotective activity of ethanolic extract of 83

Sesbania grandifolia Linn leaves
K.S.Sridevi Sangeetha
PT-OP-13 Phytochemical investigation and in-vitro anti diabetic activity of 84

Myxopyrum serratulum a. H. Hill
K. Kavitha
PT-OP-14 Energy conservation oppertunities in pharmaceutical and paper plant 85

industries using combined heat and power
Dr.N.Stalin
PT-OP-15 Antioxidant properties of Lagenaria siceraria fruit juice bagasse 86

obtained as a co-product by chemical & electrochemical assays
Sameer D Kulkarni
PT-OP-16 GC-MS characterization of volatile odorous compounds in Allium cepa 87

N.C.J.Packia Lekshmi
PT-OP-17 Isolation & evaluation of Cyperus rotundus rhizomes starch granules 88

from Jharkhand, India as a pharmaceutical adjuvant
N. Paramakrishnan
PT-OP-18 Synthesis of novel 1, 2- diazoles and evaluation of their anticancer 89

activity.
Amruta Shukla, and R.S.Jeyaprakash
PT-OP-19 Screening of aegle marmelos for antidiabetic activity in streptozotocin 90

induced diabetic rats
S.Jabaseelans
PHARMACEUTICAL TECHNOLOGY (POSTER)
PT-PP-01 Biosynthesis of silver nanoparticles using Tephrosia tinctoria for 91

antibacterial activity against multidrug resistant pathogens
D.C. Aiswarya and sureshkumar
PT-PP-02 An efficient synthesis of dihydropyrimidinone derivatives using 92

ammonium chloride-catalyzed under neat conditions for biginelli type
reaction.
M.Jayanthi
PT-PP-03 Characterization of solid state forms of pioglitazone 93

B.Poornima
PT-PP-04 Flavonoids and their medicinal significance – an update 94

K.S. Sridevi sangeetha
PT-PP-05 Guar gum: a formulator's pride 95

Amisha Vyas
PT-PP-06 Energy management and integration of PEM fuel cell with UAV for 96
pharmaceutical application
Dr.N.Stalin
PT-PP-07 A review on animal models involved drug discovery pipeline for the 97
alzheimer disease
R.Benaseer Begam
PT-PP-08 A scenario of natural plant resources on breast cancer therapy 98

M.Ponmalar
PT-PP-09 Development of validated Analytical Method for Esomeprazole and 99

Domperidone in pharmaceutical solid dosage form by HPTLC
Sunil Singh
PT-PP-10 Synthetic and pharmacological studies on a natural cyclooligopeptide 100

Suresh Kumar
PT-PP-11 Synthetic and Biological Evaluation of natural Proline rich peptide 101
Rajiv Dhiya
PT-PP-12 Comparative Analysis of total quercetin contents in aqueous and 102

ethanolic extract of Artocarpus heterophyllus by liquid
chrommatography
Joyti Shrivastava
PT-PP-13 Dendrimers based targeted drug delivery of rivastigmine for treatment 103
of Alzheimer’s disease
Krishna Reddy
PT-PP-14 Computational Graphical User Interface Tool Development for 104

Personalized Drug Selectivity and Designed Pharmacology
M.Poornima
DRUG DELIVERY SYSTEMS (ORAL)
DD-OP-01 Extraction & characterization (both physico-chemical & analytical) of 105

pectin obtained from Dillenia indica & Abelmoschus esculentus and
compatibility study with glipizide in novel drug delivery systems
Sivasankar Mohanty
DD-OP-02 Design and in-vitro evaluation of gliclazide loaded transdermal drug 106
delivery therapeutic system containing HPMC and PVP
V.Raghuraman
DD-OP-03 Formulation and in-vitro evaluation of self- emulsifying drug delivery 107
system of carbamazepine
Chinmay Vora Swati Saxena
DD-OP-04 Synthesis and characterization of novel amino acid prodrug of 108

rosiglitazone
G.Kalyana Chakravarthi
DD-OP-05 Pharmacokinetics of enrofloxacin loaded solid lipid nanoparticles 109

following oral administration in emu (Dromaius novaehollandiae) birds
P.Senthil Kumar
DRUG DELIVERY SYSTEMS (POSTER)
DD-PP-01 Sustained ophthalmic delivery of brimonidine tartrate from Thermo 110

sensitive in-situ gelling system
A.Waychal
DD-PP-02 Modified release multiparticulate delivery system for tramadol HCl 111
using gelucire 43/01 as rate retarding material
S.Divya Priya
DD-PP-03 Development and evaluation of risperidone liposomes for brain 112

targeting
Usha Y Nayak
DD-PP-04 Nanoparticle-aptamer bioconjugates: a focus on targeted cancer 113

therapy
P.Sushma
DD-PP-05 Nano-encapsulated ginsenosides: a novel approach towards targeting 114

SOD1 and TARDBP in amyotrophic lateral sclerosis (ALS)
Savithri Bhat
DD-PP-06 Development and sustainable release evaluation of enrofloxacin solid 115

lipid nanopartilces
P.Senthil Kumar
International Conference on Innovations and Future Research Dimensions on Nanobio Pharmaceutical Technology, 7-8th October, 2014
SYNERGISTIC ANTIBACTERIAL EFFICACY OF TWO DRUGS IN ADDITION WITH BIOSYNTHESIZED N

AgNPs FROM AN ALLERGENIC FUNGUS
A
Chitra n, 1B. K. Nayak, and Anima nanda
1
Department of Botany, K. M. Centre for P.G. Studies (Autonomous) N
(Govt. of Puducherry) Lawspet, Pondicherry-605008, India O
Department of Biomedical Engineering, Sathyabama University, Rajiv Gandhi Salai, Chennai - 600119, India M
Email id: bijuknayak@gmail.com Tel.: +91 9443653844
A
T
Efficacy of silver nanoparticles in order to control the growth and epidemics of pathogenic E
bacteria has been known from time immemorial. In recent days, bacteria are making themselves
R
resistant to varied antibiotics based on their genetic affinities. In the present study, a green,
I
simple and effective approach was performed to synthesize potent silver nanoparticles (AgNPs)
A
from an airborne allergenic fungus, Alternaria sp. using both reducing and stabilizing agent. The
L
appearance of yellowish brown color in the conical flask suggested the formation of AgNPs. The
supernatant of the fungus culture changed the solution into brownish colour upon the S
completion of 10 minute reaction. The characterization of silver nanoparticles was confirmed by
UV-Vis spectrophotometer, Field emission scanning electron microscopy (FESEM) and XRD
analysis. Size of the nanoparticles measured between 20nm to 30nm by FESEM. Silver
nanoparticles showed good antimicrobial activity against the selected pathogens, but when the
nanoparticles were tested against the test pathogens in combined with the two drugs viz.,
Amoxyclav/ Clavulanic Acid (30mcg) and Oxacillin (1mcg); the efficacy of the drugs was increased
at some extent by one fold in the case of Oxacillin.
Keywords: AgNPs, Alternaria sp., FESEM, XRD, Uv-Vis Spectrophotometer
Synthesis of silver nanoparticles from Alternaria sp.
1
GREEN SYNTHESIS AND CHARACTERIZATION OF HERBAL MEDIATED SILVER NANO PARTICLES N

USING GYMNEMA SYLVESTRE (Linn.)
A
Kalakotla Shanker and Gottumukkala Krishna Mohan
Centre for Pharmaceutical Sciences, IST, JNT University, Kukatpally, Hyderabad-500085. N
Email id: shankerkalakotla@gmail.com Mobile: 08185990001 O
M
Current study focuses on a novel approach towards the synthesis and characterization of A
herbal mediated silver nano particles (SNPs) using aqueous and alcoholic extracts of Gymnema T
sylvestre Linn belonging to family: Asclepiadaceae. Phytochemical screening of plant extracts E
reveals presence of phenolic compounds such as flavonoids, tannins and saponins which may R
plays an important role in formation of herbal mediated SNPs which shows anti-bacterial and I
anti-diabetic activities according to previous literature. Standard protocols have followed to A
synthesize SNPs, further the synthesized herbal mediated SNPs characterized by the various
L
instruments like SEM, EDAX, FT-IR, UV, X-ray diffraction, PSA and TGDTA. Morphology and metal
S
composition of synthesized nano particles were determined by SEM and EDAX respectively. Size,
weight variation & thermal stability of SNPs were analyzed by using PSA and TGDTA. Further the
SNPs were also characterized by UV spectroscopy, a sharp peak was observed in between 422
nm to 470 nm indicates formation of silver nano particles. Simultaneously FT-IR analysis showed
that the biosynthesized silver nano particles were capped with bimolecular compounds which
were responsible for reduction of silver, the bands seen at 3396.8 cm -1 and 2924.9 cm-1 were
assigned to the stretching and bending vibrations of secondary amines respectively. The bands
seen at 1384.8 cm-1 and 1067.8 cm-1 corresponds to –C-N- stretching vibrations. X-ray diffraction
was carried out to confirm the crystalline structure of SNPs. The synthesized SNPs were
predominately spherical in shape and poly dispersed.
Novelty of the current research: It can be concluded that the leaves of G. sylvestre can be
good source for synthesis of silver nano particles. Plant extracts have been used were eco-friendly
and thus can be cost effective. This study may be used in the development of value-added
research in the biomedical and nanotechnology fields.
Keywords: Herbal mediated silver nano particles (SNPs), Gymnema sylvestre Linn, SEM, EDAX, FT-
IR, UV, X-ray diffraction, PSA and TGDTA.
FT-IR spectra of synthesized SNPs
2
BIO-TOXICITY OF SILVER NANOPARTICLES AGAINST MULTIDRUG RESISTANT PATHOGENS AND N

HUMAN EPIDERMOID LARYNX CARCINOMA (HEp-2) CELLS
A
Muthukrishnan Lakshmipathya and Anima Nandaa
a
Faculty of Bio & Chemical Engineering, Sathyabama University, Rajiv Gandhi Road, Chennai N
Tamil Nadu 600119, India. O
Email id: animananda72@gmail.com; Tel: +914424503804; Fax: +914424502344 M
A
The nanotechnological advancement in reducing the size of noble metals (Ag, Au, Ti) with T
enhanced electrochemical properties has been the major breakthrough in materials science E
research. Biological means of synthesis especially microbe-mediated synthesis of silver
R
nanoparticles (AgNPs) using soil bacterium, Bacillus subtilis A1 and its bio-toxicity against
I
multidrug resistant strains and DNA damage has been reported. The AgNPs showed maximum
A
absorbance in the range 420 – 450nm, crystal structure and spherical shaped particles of size
L
~30.45nm. The AgNPs showed potential antibacterial activity against multidrug resistant strains
with MIC ≤ 32μg/mL. Further study on the dose dependent DNA damage using comet assay S
revealed cleaved DNA fragments in the form of comet with olive tail moment 6.4% and 1.1%
respectively at 100μg/mL and 10μg/mL respectively. This toxicological potential plays a key role
in inducing apoptosis, a limiting factor in cell death which could be harnessed for improved
biomedical application.
Keywords: Bio-toxicity, silver nanoparticles, HEp-2 cells, MIC, Comet assay
3
BIOSYNTHESIS OF SILVER NANOPARTICLES USING CLOVES OF ALLIUM SATIVUM AND ITS N

ANTIMICROBIAL ACTIVITY
A
Abirami Muniasamy1, Umamaheswari Kesavachandran1, Jagannathan G1, Aruna Sharmili2
1
Department of Biotechnology, University of Madras, Guindy, Chennai- 600 025 N
2
Department of Biotechnology, Stella Maris College, Chennai-600 086 O
Email id: rk_uma@yagoo.com Mobile: 9940326510 M
A
Synthesis of nanoparticles by biological materials is considered to be more safe, time- T
effective and environment friendly. Silver nanoparticles show both unique physicochemical E
properties and remarkable antimicrobial activities that confers to them a major advantage for R
the development of alternative products against multidrug resistant microbes. In green synthesis I
of metal nanoparticles biomolecules present in plant extracts can be used as reducing and A
capping agent to reduce metal ions into metal nanoparticles. The involved reducing agents
L
include the various water soluble plant metabolites (e.g. alkaloids, phenolic compounds,
S
terpenoids) and co-enzymes. In this study, Silver nanoparticles was synthesized by bio reduction
of silver nitrate using aqueous extract of Allium sativum and exposed to sunlight for 15 minutes.
The biosynthesized AgNPs were characterized by UV–Visible spectroscopy, HRTEM, FESEM, and
EDAX. UV–Vis spectroscopy of prepared silver colloidal solution showed absorption maxima at
409 nm which reflects surface plasmon resonance of AgNPs. HRTEM and FESEM analysis revealed
that AgNPs are spherical in shape with an average particle size of 13 nm. The synthesized SNPs
were evaluated for their antimicrobial activities against E. coli, K. pneumoniae, P. aeruginosa, S.
aureus and fungal species C. albicans, C. guilliermondii, C. krusei and C. tropicalis by micro broth
dilution method and the MIC values were recorded. The SNPs showed effective inhibition against
both bacterial and fungal species and the MIC80 values for SNPs ranged from 6.68µg/ml to
39.87µg/ml. The results suggested that SNPs exhibited excellent antimicrobial activity on all the
species tested and further evaluation on the action mechanism would provide insight on the
antimicrobial activity of these nanoparticles.
HRTEM image of Silver Nanoparticles
4
PHYSIOCHEMICAL PROPERTIES OF NANOCOMPOSITE FILM MADE OF CHITOSAN AND N

POLY-L-LACTIC ACID USING PEG AS PLASTICIZER
A
Surumi B a, Tincy K Thomas b, Neethu Hari band A. Jayakumaran Nair b
a
Centre for Nanoscience and Nanotechnology, University of Kerala, Thiruvananthapuram N
b
Department of Biotechnology, University of Kerala, Thiruvananthapuram, Kerala-695 581. O
Email id: jekksnair@gmail.com, Mob: +919447059164 M
surumisubair@gmail.com, Mob: +919447439825
A
T
Nanostructures especially nanocomposites and nano thin films have enormous
E
applications now a days. Nanocomposite film from chitosan and Poly-L-Lactic acid were prepared
R
by solution mixing and cast drying. PEG was used as plasticizer for proper film blending.
Physicochemical properties like its stability, solubility, swelling, porosity and moisture content I
were studied along with characterization using XRD, UV spectroscopy, FTIR and PL analyses. A
Antibacterial efficiency of film was analyzed using 3 bacterial strains namely Escherichia coli, L
Bacillus subtilis and Staphylococcus aureus by growth kinetics method, in order to suggest the S
same as a packaging material or for biomedical applications. Nanocomposite film showed UV
absorption around 294nm and is crystalline in nature with grain size of about 57.47nm offers
great advantage in preventing surface bacterial growth on it because of its antibacterial activity
hence could be used for various applications. Drug loading and in vitro drug release by these films
were also tested and results suggest that these films are excellent candidates for controlled drug
delivery applications.
Nanocomposite film (1) physical appearance (2) Light Microscopic view (40X)
5
LARVICIDAL AND ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES FROM A POTENT N

ACTINOMYCETE, SACCHAROPOLYSPORA ERYTHRAEA
A
Prabha S. B. And K. Murugesan
CAS in Botany, University of Madras, Guindy campus, Chennai- 25, Tamilnadu, India. N
E-Mail id: pkprabha27@gmail.com Mob: 9445319254 O
M
The actinomycetes have the ability to produce secondary metabolites. The biosynthesis A
of silver nanoparticles using terrestrial actinomycete Saccharopolyspora erythraea is the first and T
novel report. Although actinomycetes are well known for its antibiotic production, it is less E
exploited in terms of production of nanoparticles. The silver nanoparticles are characterized R
using several analyses such as UV spectrophotometer, XRD, FTIR, FE-SEM and HR-TEM. The silver I
surface plasma resonance was observed at 450nm which confirmed the formation of AgNP’s. The A
XRD pattern confirmed the formation of nanocrystals. The FTIR analysis is involved in the
L
reduction of silver salts into respective nanoparticles. In FE-SEM with EDX, the average size of the
S
nanoparticle was found to be 82.13 to 93.86nm and it confirmed the chemical analysis of the
fields. The HR-TEM analysis with SAED showed that the synthesized AgNP’s were polydispersed
in 6 to 26 nm scale which confirmed the formation of AgNP’s. The antimicrobial activity of the
ethyl acetate extract of the isolate Saccharopolyspora erythraea (AMP14) amended with that of
the SNPs gave good results and showed maximum zone of inhibition against the five human
pathogen namely Bacillus subtilis, Klebsiella pneumoniae, Vibrio cholerae, Salmonella typhi and
Staphylococcus aureus. The ethyl acetate extract of the isolate Saccharopolyspora erythraea
(AMP14) was found to be less toxic than that of the synthesized silver nanoparticles against the
larvae of the Anopheles stephensi and Aedes aegypti the larvicidal activity was the first report of
Saccharopolyspora erythraea.
Keywords: Saccharopolyspora erythraea, silver nanoparticle, larvicidal activity, antimicrobial

activity, HR-TEM. Anopheles stephensi, Aedes aegypti.
FE-SEM with EDX image of AgNPs synthesized by Saccharopolyspora erythraea.
6
FORMULATION AND EVALUATION OF MOUTH DISSOLVING FILM LEFLUNOMIDE FOR N

RHEUMATOID ARTHRITIS
A
S. Lakshmana Prabu, A. Shanmugarathinam, S.P. Saravanan, M.Elakkiya, V. Manikandan,
A. Bhuvaneswari, S. Aravindan N
Dept. of Pharmaceutical Technology, Bharathidasan Institute of Technology, O
Anna University, Tiruchirappalli – 620 024. M
A
The present work aimed to prepare mouth dissolving films of leflunomide with the T
purpose of developing a dosage form for a very quick onset of action, which is very convenient E
for administration, without the problem of swallowing and using water. The films of leflunomide R
were prepared by using polymers such as PVA and HPMC using PEG as plasticizer, by solvent I
casting method. Drug excipient compatibility was studied by FTIR and UV techniques. The
A
formulated mouth dissolving films were evaluated for physical characteristics such as uniformity
L
of weight, thickness, folding endurance, drug content uniformity, surface pH, in-vitro drug release
S
tests and stability study. Mouth dissolving film of leflunomide containing PVA as polymer showed
99.18 % drug release at 30 min. Stability studies revealed that optimized formulation was stable.
Keywords: Leflunomide, mouth dissolving film, solvent casting, rheumatoid arthritis
Comparative invitro release studies of formulation F1 to F5
7
ANTIMICROBIAL, ANTIOXIDANT AND ANGIOGENIC POTENTIAL OF SILVER NANOPARTICLES N

FROM LEAVES OF MURRAYA PANICULATA (L.) JACK A
Rama. P, Vignesh. A and K. Murugesan N
Centre for Advanced Studies in Botany, Guindy Campus, University of Madras, Chennai O
Email id: deeparamya48@gmail.com Mobile: 7708908195
M
A
Green synthesis of silver nanoparticles was carried out using leaves of Murraya paniculata T
and was used as a reducing agent. Biosynthesized AgNPs were characterized by UV–Vis, FESEM, E
TEM-EDAX, XRD and FT-IR spectroscopy techniques. It was tested for their antimicrobial activity
R
against human pathogens. In vitro radical scavenging assay and reducing power assay was tested.
I
Cytotoxicity of biosynthesized AgNPs against in vitro human endothelial vein cell (HUVEC) line
A
was analysed. Enhancement of cell migration was determined by scratch wound assay and
L
expression of VEGF by HUVEC was examined using western blotting analysis. UV–Vis
spectroscopy of AgNPs showed absorption maxima at 438 nm. Characterization of AgNPs S
revealed that it is face-centered cubic structure, spherical shape with an average particle size of
23 nm. Among the human pathogens viz., Bacillus cereus, Staphylococcus aureus, Candida
albicans and Escherichia coli, AgNPs demonstrated highest sensitivity toward Bacillus cereus.
AgNPs containing leaf extract showed good antioxidant activity than extract and standard
ascorbic acid. Cytotoxicity of biosynthesized AgNPs against in vitro human endothelial vein cell
line showed dose-dependent activity. It was observed that AgNPs at 15.625 μg/ml concentration
could stimulate proliferation and migration of endothelial cells (EC). The expression of VEGF by
EC in response to synthesized AgNPs might provide an important mechanism by which EC
migration and permeability is regulated. This result indicates that AgNPs possess antimicrobial,
antioxidant and angiogenic activities, which may lead to the development of nanomedicine for
the treatment of diseases.
Keywords: Murraya paniculata, X-ray diffraction analysis, vascular endothelial growth factor.
SAED pattern of AgNPs from leaves of M. paniculata.
8
ANTIOXIDANT AND HEMOLYTIC PROPERTY OF SILVER NANOPARTICLES SYNTHESIZED USING N

ALLIUM SATIVUM
A
Neethu Hari*, Tincy K Thomas and A. Jayakumaran Nair
Department of Biotechnology, University of Kerala, Kariavattom campus, Thiruvananthapuram – 695581, Kerala N
Email id: jekksnair@gmail.com, Mob: +919447059164 O
neethuharisharon@gmail.com, Mob: +919895698888 M
A
T
An eco-friendly biogenic synthesis of inorganic nanoparticle is a fast growing research in E
the field of nanotechnology. This study emphasis on the green synthesis of silver Nanoparticles
R
(Ag NPs) using commercially available Garlic (Allium sativum) without the use of toxic chemicals.
I
This is achieved by reducing silver nitrate (AgNO3) solutions under ambient conditions by using
A
the extract of Allium sativum. This extract will act as reducing as well as capping agent. Visually,
L
the formation of stable silver Nanoparticles (Ag NPs)of fairly well-defined dimensions were
confirmed by observing the colour changes from yellow to reddish brown colour and an intense S
peak was observed in the UV- spectrophotometer at 425 nm. Further, formation of silver
nanoparticles was confirmed by XRD, FTIR and SEM analysis. Crystalline nature of the Silver
nanoparticles synthesized using Allium sativum was confirmed by the peaks in the XRD pattern
corresponding to (111), (200) and (220) planes. Fourier transform infrared spectroscopy (FTIR)
showed that silver nanoparticles were capped with plant compounds. Allium sativum were
revered to possess powerful antioxidants, sulfur and other numerous phenolic compounds which
arouse great interests. Here the antioxidant capacities of silver nanoparticles synthesized using
Allium sativum (As-Ag NPs) were determined by DPPH radical scavenging assay and reducing
power assay and these Phytosynthesized silver nanoparticles exhibited strong antioxidant
activity. We also investigate the importance of in vitro tests for evaluation of the compatibility of
Ag NPs with human blood. Hemolytic activity of green synthesized silver nanoparticle was
compared with chemically synthesized silver nanoparticles .The results showed that green
synthesized Silver nanoparticles exhibits less than 50 % haemolysis, which can be considered as
haemocompatabile
80
70
60
Hemolysis (%)
50
40 Cs-Ag NPS
30
As-Ag NPs
20
10
0
50 100 150 200 250
Concentration (µg/mL)
Fig 1: Hemolytic activity of green and chemically synthesized Silver nanoparticles
Fig 2: SEM Analysis of green synthesized Silver nanoparticles (As-Ag NPs)
9
TINIDAZOLE LOADED NANOPARTICLES FOR TREATMENT OF PERIODONTITIS: FORMULATION, N

OPTIMIZATION AND CHARACTERIZATION
A
M.Rajput, D.Nagane, S.Dhat
Department of Pharmaceutics, S.T.E.S.’s Sinhgad Institute of Pharmacy, Pune, Maharashtra, India N
Email id: mahipalrajput.07@gmail.com, shalakapd@gmail.com; O
Mob: +919595632007, +919823270454 M
A
In the present work attempt has been made to formulate and characterize locally acting T
tinidazole (TNZ) loaded nanoparticulate delivery system for the treatment of periodontitis. E
Nanoparticles of TNZ were formulated by nanoprecipitation technique using Eudragit RL 100 as
R
encapsulating polymer and tween 80 as stabilizer. A 23 Box behnken design was used to study
I
the effect of varying concentration of Eudragit RL100, amount of aqueous phase and amount of
A
tween 80 on particle size and entrapment efficiency. The optimized formulation was lyophilized
L
using 2% sucrose as cryoprotectant. The particle size and PDI of lyophilized nanoparticles were
found to be 294 ± 0.5nm and 0.233 respectively. Zeta potential of lyophilized nanoparticles S
(+24.19mV) seemed to be promising for achieving the desired stability and therapeutic effect. In
vitro release studies of lyophilized nanoparticles in McIlvaine buffer (pH6.6) showed release of
TNZ in two phases; an initial burst release (25.73%) in the first two hours followed by slow release
of remaining drug (93.53%) over a period of 24 h. The kinetics of release of TNZ from nanopaticles
followed Higuchi release. Based on the value of ‘n’ (diffusion exponent), the mechanism of TNZ
release was found to be quasi fickian diffusion. MIC and MBC studies of the formulation showed
ten folds and five folds reduction in MIC and MBC respectively as compared to pure TNZ. Based
on the above studies it can be concluded that TNZ loaded nanoparticles can be a potential
delivery system for the treatment of periodontitis.
MIC of pure TNZ (A2), Lyophilized NPs (B2) and blank NPs (C2).
10
DEVELOPMENT OF SEMISOLID DRUG CARRIERS FOR TOPICAL APPLICATION BASED ON N

SOLID LIPID NANOPARTICLES
A
Prajakta Sarode, Pankaj Desai, Manasi Ghag, Swapnila Shinde
STES’s Sinhgad Institute of Pharmacy, Narhe, Pune-411041. N
E-mail id: Prajaktasarode17@gmail.com Mobile: 91-9011124709 O
M
Osteoarthritis and rheumatoid arthritis are auto immune diseases distinguished by A
cartilage degeneration and bony overgrowth. Worldwide, osteoarthritis (OA) and rheumatoid T
arthritis (RA) are estimated to be the fourth leading cause of disability. Celecoxib is selective COX E
– 2 inhibitor approved for its use in treatment of osteo and rheumatoid arthritis. Poor solubility R
of drug along with life threatening side effects shown by conventional system can be overcome I
by use of topical solid lipid nanoparticles. This system offers local action of drug for prolonged A
time with prominent dose reduction. Glyceryl Monostearate (GMS) was used as drug soluble lipid
L
with Tween 80 as oil-in-water surfactant. The 32 factorial design was used to study the effect of
S
independent variables viz. concentrations of lipid (GMS) and surfactant (Tween 80), on
dependent variables like particle size and entrapment efficiency (%EE). Optimized batch showed
81% entrapment efficiency and an initial burst release of drug i.e 11.42% till 3h and was followed
by sustained release pattern reaching 79.40% of at end of 24h. Optimized formulation was
evaluated for various parameters such as thermal analysis, accelerated stability studies as per
ICH (Q1A), in vitro drug release studies, ex vivo permeability studies, in vitro skin occlusivity test
and histological examination. It was found that developed formulation exhibited faster onset of
action with prolonged drug release and was stable, non-irritant, and therapeutically effective.
Hence, Celecoxib loaded solid lipid nanoparticles can be considered as promising alternative to
conventional topical systems.
11
IMPROVEMENT OF PHYSICOCHEMICAL PROPERTIES AND ANTIANGIOGENIC EFFICACY OF N

ACTARIT BY RAPID DISSOLVING NANOEMULSIFYING TECHNIQUE
A
Shraddha Hidadugi, Ajinkya Khawade, Rohit Thakur, Swati Pund
STES’s Sinhgad Institute of Pharmacy, Narhe, Pune 411041 N
Email- hidadugi.shraddha43@gmail.com, Mobile: 07757898335 O
M
Actarit, an orally administered disease modifying anti-rheumatic drug is used in the A
treatment of rheumatoid arthritis. Actarit exhibits poor aqueous solubility. In order to improve T
poor water solubility and limited dissolution rate, novel formulations are needed. Among various E
methods to increase drug solubility, self-nanoemulsifying drug delivery system (SNEDDS) has R
received particular attention as a means of enhancing oral bioavailability of poorly soluble drug. I
Present study describes the development of SNEDDS of Actarit in liquid as well as solid forms that A
resulted in improved solubility and dissolution. Solubility of the Actarit was determined in various
L
oils, surfactants and co-solvents. Pseudo-ternary phase diagrams were constructed to identify
S
the most efficient self-emulsification region. The composition of SNEDDS used for Actarit
formulations in liquid and solid forms contained mixture of surfactants: cosurfactant; Labrasol
and ethanol and oil; Acrysol K 150, as these excipients showed maximum solubility of Actarit. The
formulations were characterized for various physicochemical characteristics like particle size,
zeta potential, dissolution studies, cloud point, emulsification time, assay and thermodynamic
stability. Liquid as well as solid formulation (liquid adsorbed on Florite RE) rapidly formed fine oil-
in-water nanoemulsion, with particle size ranges 10 nm– 142 nm with zeta potential around –
4.31 mV. Emulsification time was less than one minute and the cloud point was > 80 °C. The in-
vitro rate and extent of release of Actarit from solid SNEDDS was significantly higher than Actarit
alone. Thermodynamic stability studies showed that liquid SNEDDS of Actarit were stable to three
freeze and thaw cycles and showed no precipitation even after centrifugation. Strong
antiangiogenic activity of actarit was discovered and confirmed by in vivo Chick Chorioallantoic
Membrane assay. These studies demonstrate that the new SNEDDS of Actarit is a promising
strategy for improving its solubility and in turn, bioavailability.
Particle size analysis and zeta potential of acatrit nanoemulsion
12
IN SITU NANOEMULSIFYING BIOACTIVE PHYTOCONSTITUENT CHRYSIN FOR IMPROVED N

PHYSICOCHEMICAL CHARACTERISTICS AND IN VITRO CYTOTOXICITY
A
Deepali Divate, Shahsikant Gangurde, Swati Pund
STES’s Sinhgad Institute of Pharmacy, Narhe, Pune 411041 N
Email- divate.deepali@gmail.com, Mobile. 07798890531 O
M
A
Chrysin, a naturally occurring flavone has attracted considerable interest for its beneficial T
potentials for human health, which include anti-oxidant, anti-inflammatory, cardioprotective and E
anti-tumor activities. Despite outstanding advancements in fundamental biology; chrysin is not R
yet progressed from ‘bench to bedside’ for human use due to poor bioavailability. In order to I
achieve a maximum response of natural products for human use, strategies are required that can
A
improve upon the solubility and bioavailability. The nanotechnology based delivery system
L
circumvents solubility and bioavailability issues. Chrysin has extremely poor aqueous solubility;
S
~1 µg/ml. We have developed self nanoemulsifying preconcentrates for chrysin comprising of
Capryol 90, Tween 80 and Transcutol HP which showed significantly higher solubility of chrysin;
18 ± 0.44 mg/ml. This system on dilution with water rapidly generated nanoemulsion with a
globule size of 174.12 nm and zeta potential – 15 mV. The formulation was robust to pH change
and dilution. Cloud point was found to be well above 65°C and the formulation was also stable
towards thermodynamic stress testing. These studies indicate that nanoemulsification has
significantly improved the solubility and stability of chrysin. Despite outstanding advancements
in fundamental biology; chrysin is not yet progressed from ‘bench to bedside’ for human use. In
order to achieve a maximum response of natural products for human use, strategies are required
that can improve upon the solubility. The nanotechnology based delivery system circumvents
solubility and bioavailability issues. The developed formulation showed rapid dissolution and
enhanced in vitro cytotoxicity activity in MCF-7 breast cancer cell line. Briefly, in situ
nanoemulsifying chrysin dramatically enhanced the solubility, dissolution and anticancer activity,
thus increasing its potential application in cancer chemotherapy.
Particle size analysis in situ emulsifying formulation of chrysin
13
ANTIBACTERIAL POTENTIAL OF ZINC OXIDE NANOPARTICLES SYNTHESIZED USING WET N

CHEMICAL METHOD
A
T Sudhakar, Anima Nanda, J Premkumar, Jithumon P James, Jokutty Thomas, Mathews abraham
Department of Biomedical Engineering, Faculty of Bio & Chemical Engineering, N
Sathyabama University, Chennai 600 119. O
Email id: tsr.182001@yahoo.co.in +91 9840401909 M
A
The present research work, synthesis of zinc nanoparticles and its characteristization T
was done. In this study, zinc nanoparticles were rapidly synthesized from zinc acetate and E
sodium hydroxide solution using soluble starch and formation of nanoparticles observed with R
wet chemical method. The synthesized zinc nanoparticles were characterized by different I
spectroscopic and analytical techniques such XRD and SEM. X ray diffraction and SEM analysis A
studies confirmed the formation of well dispersed zinc nanoparticles with average particle size L
the range 10 nm as well as revealed their hexagonal structure. These biologically synthesized S
nanoparticles were found to be toxic against different multi drug resistant human pathogens.
Therefore synthesized zinc nanoparticles were used to study the antimicrobial activity against
Enterococcus fecalis, Shigella dysentriae, Staphylococcus aureus, and Salmonella paratyphi by
using well-diffusion and disc diffusion methods.
SEM image for ZNO nanoparticles of scale 100nm.
14
GREEN SONOCHEMICAL SYNTHESIS OF SILVER NANOPARTICLES AND THEIR APPLICATIONS N

Dr. I. Johnson and H. Joy Prabu
Centre for Nano Science and Applied Thermodynamics, Department of Physics, St. Joseph’s College (Autonomous),
A
Trichy-620002, India Email Id: joyprabuphy@gmail.com N
O
Silver nanoparticles were synthesized at room temperature by a green sonochemical M
process without any surfactants and templates then silver nitrate as silver precursor. The A
products are characterized by X-ray diffraction (XRD), ultraviolet-visible spectroscopy, scanning T
electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The synthesis is E
performed conveniently and safely. The results show that under certain conditions Ag R
nanoparticles have good stability and uniform size. This method is convenient, easy and effective I
in comparison to the known methods of the synthesis of nanomaterials. Besides simplicity, the A
advantages of producing nanoparticles by this method are that it is easeful, flexible, fast, cost
L
effective, and pollution free.
S
SEM image of nanoparticles
15
PRODUCTION AND CHARACTERIZATION OF FUNGAL ASPARAGINASE BOUND SILVER N

NANOCOMPOSITE - A POTENT NANODRUG FOR LYMPHOCYTIC LEUKEMIA
A
G. Baskar*, Garrick Bikku George, M.Chamundeeswari, R. Aiswarya
Department of Biotechnology, St. Joseph’s College of Engineering, Chennai – 600 119. N
Emaid id: basg2004@gmail.com O
M
Nanobiotechnology is an integral part in the field of pharmacy where biotechnologists A
play a pivotal role in production of nanoparticles and nanomaterials using biomaterials for T
various biomedical applications. Fungal L-asparaginase has many advantages in the treatment of E
lymphocytic leukemia with lesser side effect as the free circulating free asparagine are converted R
into aspartic acid and ammonia by asparaginase thereby leaving the cancerous cells devoid of I
growth. In the present work nanobiocomposite of fungal asparaginase was produced using A
glutaraldehyde pretreated colloidal silver nanoparticles. The formation of silver
L
nanobiocomposite of asparaginase was observed using laser light scattering method and
S
confirmed using UV-Visible Spectrophotometer with the absorption peaks at 362, 400 and 450
nm. The average size of the produced nanobiocomposite was found as 60 nm using Field Emission
Scanning Electron Microscope. The specific asparaginase activity was increased from 252.05
U/mg of crude asparaginase to 303.66 U/mg in nanobiocomposite of asparaginase. Hence the
presence of asparaginase on the synthesized nanobiocomposite was confirmed and it can be
used as an effective anticancer drug with increased bioavailability.
Keywords: Asparaginase, Lymphocytic leukemia, Nanodrug, Characterization,

Nanobiocomposite.
16
FORMULATION AND IN VITRO - IN VIVO EVALUATION OF WEDELOLACTONE LOADED N

NANOSUSPENSION FOR HEPATOPROTECTIVE ACTIVITY IN CCL4 INDUCED SIGNIFICANT
A
HEPATIC DAMAGE AND OXIDATIVE STRESS MODEL
N
S.Brito Raj1, P.Sucharitha*1, T.Murali1, S.Wasim Raja2 and K.Bhaskar Reddy1
Department of Pharmaceutics, Sri Venkateswara college of Pharmacy, RVS Nagar, Chittoor - 517 127, O
Andhrapradesh, India. M
Department of Pharmacy Practice, Sri Venkateswara college of Pharmacy, RVS Nagar, Chittoor - 517 127, A
Andhrapradesh, India.
T
Email id: sucharitha89pharma@gmail.com Mob: 09052879033
E
Nanosuspension have emerged as a promising strategy for the efficient delivery of R
hydrophobic drugs because of their versatile features and unique advantages. The aim of I
present study was to develop Nanosuspension of poorly water soluble drug wedelolactone by A
using a combination of solvent evaporation followed by high-pressure homogenization and it was L
an optimized technique for the preparation of Nanosuspension, which lead to better results like S
high efficiency, high drug content. Prepared Nanosuspension was evaluated for drug content,
SEM, particle size distribution, zeta potential, poly dispersity, in-vitro drug release, and in-vivo
hepatoprotective activity by CCl4 induced significant hepatic damage and oxidative stress model.
The results Shows that the prepared Nanosuspension persists good stability with 98.91% drug
content, Droplet size was found to be 115.9 nm with poly dispersive index 0.263 and -3.04 mV of
zeta potential. This indicates that nano suspension is a stable formulation. SEM photography
showed smooth surface and spherical in shape. The drug release of Nanosuspension formulation
is about 97.72 % which indicates the increase in dissolution rate of wedelolactone compared to
conventional dosage forms.
SEM- Surface Morphology of Nanosuspension
17
ANTIDIABETIC ACTIVITY OF CHROMIUM (III) NANOPARTICLE AGAINST STREPTOZOTOCIN- N

INDUCED DIABETIS IN RATS
A
Kanakalakshmi Annamalai1*, Shanthi Kuppusamy1
1
Department of Environmental Science, PSG College of Arts and Science, Coimbatore-641 01 N
E mail: akanakalakshmi1988@gmail.com O
M
The usage micronutrients for medical complications are gaining more popularity in now a A
days. Among the micronutrients, chromium is an essential mineral which plays vital role in T
glucose metabolisms and gaining new insights in to its versatile applications in treating metabolic E
disorders like type II diabetes mellitus. To support this concept very few experiments had been R
conducted in animal models. In view of the above fact the present study was aimed to explore I
the antidiabetic activity of chromium III nanoparticles against STZ (65 mg/kg i.p) induced diabetes
A
in rats. In diabetic rats, the chromium III nanoparticles were administrated on daily basis at the
L
dose range of 30 mg/kg p.o for about 28 days. The levels of blood glucose and body weight of the
S
animal were measured periodically. STZ treated diabetic rats showed significant alteration in the
biochemical parameters and this elevation were restored (p<0.5) to near normal by chromium III
nanoparticle treatment. In conclusion, the results from the present study indicates that
chromium III possess antidiabetic activity against STZ model in rats.
TEM image of Chromium III nanoparticles
18
INVITRO CYTOTOXICITY ASSESSMENT OF SILVER NANOPARTICLES SYNTHESIZED USING N

ASPERGILLUS TERREUS AGAINST BREAST CANCER CELL LINE
A
M.D.Bala Kumaran and P.T.Kalaichelvan
CAS in Botany, University of Madras, Chennai – 600 025, India N
Email id: dakshinbala@gmail.com, Mob: +919500077165 O
M
Biosynthesis of nanoparticles is under exploration due to their wide range of biomedical A
applications and research interest in nanotechnology. Nano-sized particles with antimicrobial T
and anticancer properties are of great interest in the field of medical research. The present study E
focuses on the extracellular biosynthesis of Silver nanoparticles (AgNPs) from silver nitrate R
solution using Aspergillus terreus, isolated from soil. The biologically synthesized silver I
nanoparticle was tested for its anticancer property against breast cancer (MCF) cell line. The A
fungal extract was reacted with AgNO3 and the synthesis of nanoparticles was screened using
L
UV–Vis spectroscopy. The synthesized nanoparticles were then characterized using XRD and TEM
S
equipped with EDS and the size of the silver nanoparticles was found to be spherical in shape
with 5 to 20 nm. The invitro cytotoxicity of silver nanoparticles toward MCF cell lines was
assessed by MTT assay using dose dependant approach. The apoptosis property was studied by
acridine orange and eithidium bromide staining method and the biologically synthesized silver
nanoparticles showed good cytotoxicity activity against breast cancer cell line with an IC50 value
of 35.8 µg/ml.
Keywords: Silver nanoparticles, Aspergillus terreus, MCF Cell line
TEM image of AGNPs AO/EB staining of MCF cell line treated with AGNPs
19
A NOVEL FENUGREEK DERIVED VICENIN-2 GOLD NANOPARTICLES AND ITS GLUCOSE UPTAKE N
EFFECT IN 3T3-L1 ADIPOCYTES
A
C. Shivashri, T. Rajarajeshwari, P. Rajasekar
Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai-602 105, Tamil Nadu N
Email id: shivashri.cj@gmail.com, rajarajeshwari.t@gmail.com O
M
The anti-diabetic property of bioactive constituents of fenugreek has been well A
established. Thus, the study synthesized gold nanoparticles using Vicenin-2, a C-glucoside T
flavonoid of fenugreek. The biosynthesized vicenin-2 gold nanoparticles (V-AuNPs) were E
confirmed primarily by its color transformation and UV absorption. The size and shape, crystalline R
nature and stability of V-AuNPs were examined under TEM, XRD and FT-IR analysis respectively. I
The bio-reducing efficiency of vicenin-2 was computed. The stability of V-AuNPs in various A
physiological medium (10% NaCl, 0.5% BSA, 0.2 M Histidine & 0.2 M Cysteine) and different pH
L
(1.2, 5, 7.4 & 9) was explored. The cytotoxicity and glucose uptake assays were also performed
S
in 3T3-L1 adipocytes. The appearance of yellowish orange color and UV absorption at 537 nm
confirmed the formation of V-AuNPs. The V-AuNPs showed a spherical morphology with a size of
57 nm and appeared as face centered cubic (fcc) crystals under TEM and XRD analysis
respectively. The FT-IR spectra of plain Vicenin-2 and its gold nanoparticles confirmed functional
groups responsible for the biosynthesis of V-AuNPs. The reducing potential of Vicenin-2 was
found to be 97.5%. The V-AuNPs showed a remarkable stability in different physiological medium
and in buffers of different pH. The IC-50 value for V-AuNPs was found to be 119 µg/µL. A
concentration dependent rise in glucose uptake was noted in 3T3-L1 adipocytes on treatment
with V-AuNPs. The study suggests that V-AuNPs would serve as a novel nano-based bio-drug for
the management of diabetes.
Keywords: Vicenin-2, Gold nanoparticles, Characterization, Stability, 3T3-L1 adipocytes,

Cytotoxicity, Glucose uptake
20
PHYTOGENIC SYNTHESIS OF SILVER NANOPARTICLES AND ITS POTENTIAL FORON BLUE DYE N
DECOLORIZATION
A
P. Balashanmugam1*, P. Arthi 2 and P.T Kalaichelvan3
1
Centre for Advanced Studies in Botany, University of Madras, Chennai 600 025, India N
2
Department of Inorganic Chemistry, University of Madras, Chennai 600 025, India O
M
3
ALKA Research Foundation, Coimbatore, 641 046, India
Email id: biobala17@gmail.com, 91+9994633730
A
T
Textile industries are the major users of dyes in the world. A huge fraction of dyes are
E
discharged out from the textile industries, causing serious damage to the environment.
R
Nanotechnology based technologies has been proved to be the most attractive and cost-
effective method to counter textile dye pollution. The ability of the Silver nanoparticles (Ag NPs) I
to decolorize dyes the textile dyes has been investigated. A novel bio approach for the synthesis A
and stabilization of Ag NPs using aqueous extract of Cassia roxburghii leaves under ambient L
conditions. The instant formation of Ag NPs was analyzed by visual observation and UV–visible S
spectrophotometer. The synthesized Ag NPs were characterized by HR-TEM with EDX and XRD.
Appearance of dark brownish color confirmed the formation of Ag NPs. Phytoconstituents
present in the plant extract might have helped in the reduction and capping agent of Ag NPs. The
XRD and EDX pattern of synthesized Ag NPs showed their crystalline structure with face centered
cubic and silver metals are present in the nanoparticles. HR-TEM studies exposed that the
diameter of stable Ag NPs was approximately 40 nm. These particles were then checked for their
efficiency to decolorize the textile dye foron blue. The Ag NPs are observed to be have a good
catalytic activity on the reduction of foron blue. The nanoparticles treated effluent was tested on
seed germination and growth of green gram. The treated dyes also improved seed germination
and growth of green gram when compared to the untreated dyes. This indicated the nontoxicity
of the Ag NPs decolorized dye effluent respectively.
Keywords: Bioreduction, silver nanoparticles, foron blue, decolorization, seed germination.
21
ENTRAPMENT OF SCOPARIA DULCIS EXTRACT IN CHITOSAN ALGINATE NANOPARTICLES FOR N

DEVELOPMENT OF ANTIMICROBIAL MEDICAL TEXTILES
A
Rajalakshmi V, R. Rajendran, R. Radhai*, Josena George
PG & Research Department of Microbiology, PSG College of Arts and Science, Coimbatore. N
Email id:radhai.research@gmail.com O
M
Nanotechnology has unscrewed the door for development of various new materials, wide A
range of industrial and consumer applications. This research provides a poignant example of the T
expanding use of nanotechnology and nanomaterials. The present investigation emphasizes on E
screening of herbal extract with potent antimicrobial activity, preparation of nanoparticles of R
their extracts, treatment of cotton fabrics with herbal nanoparticles, and assessment of their I
antimicrobial efficacy. In the present work ethanol, methanol, petroleum ether and water A
extracts of the leaves of Scoparia dulcis were screened for their anti-microbial activity by using
L
the agar diffusion method. The minimum inhibitory concentration of the extracts was also
S
measured. The methanol extracts S.dulcis proved to have the maximum antimicrobial effect were
loaded inside the chitosan alginate nanoparticles by cation induced controlled gellification
method. The average particle size of the nanoparticles was calculated using dynamic light
scattering technique (DLS). The Chemical Characterization of the Herb loaded nanoparticle was
analyzed using FTIR. Further herb loaded nanoparticle was finished on cotton fabric by pad dry
cure method. The antimicrobial antifungal activity of the fabrics was assessed by using the
standard AATCC technique (AATCC 100, AATCC 147, and AATCC 30). The quantitative tests proved
that cotton fabrics finished with the methanol extract of S. dulcis loaded nanoparticles possessed
remarkable antibacterial activities. The study revealed that the herb encapsulated nanoparticle
could act as biocontrol agents.
Average particle size of the nanoparticles Loaded Antifungal activity of treated cotton fabrics – Soil uri
with S.dulcis leaf extract
22
CHARACTERISTIC FORMULATION OF SOLID LIPID NANOPARTICLE ENCAPSULATED N

THYMOQUINONE AND ITS APPROACH ON IN-VIVO PHARMACOKINETICS AND
A
BIODISTRIBUTION TOWARDS TARGETING BRAIN DRUG DELIVERY
N
Dr.T.Sumathi and R.Surekha, Department of Medical Biochemistry, Dr.ALM Post Graduate
Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, Tamil Nadu O
Email id: sumsthangarajan@gmail.com M
A
The aim of the present research is to formulate and characterize solid lipid nanoparticles T
(SLNs) of Thymoquinone (TQ-SLNs) for the effective treatment of neuro-related diseases. TQ- E
SLNs were prepared using hot homogenization followed by micro emulsion method to get the R
desired particle size having maximized entrapment efficiency as well as percentage release. I
Optimized TQ-SLNs with appropriate characteristics (particle size = 172.1 ± 7.41 nm; zeta A
potential = -45.4 ± 2.68 mV; entrapment efficiency = 84.495 ± 3.361%) were fabricated. The in-
L
vitro drug release data revealed a maximum of 86.151 ± 2.766% for TQ-SLNs and 45.52
S
± 3.975% for TQ suspension (TQ–S) in 72 hrs, which suggests Korsmeyer-Peppas and Higuchi
models of kinetic release mechanisms respectively. Morphology of the formulations were
observed by TEM and revealed a relatively spherical surface of SLNs. The state of the formulation
was characterized by XRD and it exhibited the reduced crystallinity of the entrapped drug. In-vivo
pharmacokinetic studies showed nearly 5 fold increase in the bioavailability of TQ-SLNs (AUC0→∞
2588.992 ± 112.361 µg/ml/h) as compared with that of TQ–S (AUC0→∞ 489.841 ± 27.752
µg/ml/h) after oral administration in rat plasma. Drug distribution of TQ-SLNs in various vital
organs was found to be in the following range: brain (AUC0→∞ 66.921 ± 2.505 µg/g/h) > liver
(AUC0→∞ 21.704 ± 2.395 µg/g/h) > kidney (AUC0→∞ 17.441 ± 1.946 µg/g/h) > heart (AUC0→∞
13.461 ± 2.548 µg/g/h)> spleen (AUC0→∞ 11.4 ± 3.641 µg/g/h) > lung (AUC0→∞ 1.118 ± 3.725
µg/g/h). On the other hand TQ–S showed reduced AUCs in all the organs when compared with
that of TQ-SLNs. These above results collectively suggests that targeting the drug towards brain
was enhanced with lipid coated TQs when compared with that of normal TQs. Further studies on
TQ-SLNs with respect to treat neurodegenerative diseases are warranted.
TEM image of nanoparticles XRD pattern of TQ-SLNs
23
SYNTHESIS AND CHARACTERIZATION OF ANDROGRAPHIS PANICULATA LOADED N

NANOPARTICLES FOR THE DEVELOPMENT OF ANTIMICROBIAL POLY COTTON FABRICS
A
K. Hemalatha, R. Rajendran, A.Manikandan
PG & Research Department of Microbiology, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India. N
Email id: kbghema@gmail.com O
M
The present study was focused on the development of herbal based nanoparticle finishes A
on Polycotton fabric for protective purpose. Andrographis paniculata was used to study the T
antimicrobial property. Methanolic extract of this plant showed the higher antimicrobial activity. E
MIC was performed with plate titre method against gram positive (Staphylococcus aureus, R
Bacillus subtilus, MRSA) and gram negative microorganisms (Escherichia coli, Pseudomonas I
aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, Serratia marcescens, Proteus A
vulgaris) respectively. Antifungal study was done with Agar plug method against Aspergillus
L
niger, Trichoderma reesei and Penicillium notatum. Plant extract was checked for the presence
S
of phytochemical components. GCMS and FTIR analysis were done with methanolic extract of A.
paniculata. Nanoparticles were prepared by using emulsion technique. Physical and chemical
characterizations were done with the synthesized nanoparticles. Average size of the
nanoparticles was determined as 164.5nm by DLS analysis. Shape of the nanoparticles was
determined by HRSEM analysis. Nanoparticles were coated on the fabric by pad-dry cure method.
Antibacterial activity of the coated fabric was checked using AATCC 147 and AATCC100 (Figure:
1). the antibacterial efficacy of the fabric was reached up to 35 washes. Antifungal activity was
checked using AATCC 30, humidity jar method and soil burial test. Treated fabric was showed
higher antimicrobial activity. The physical parameters of the treated fabric showed good results
when comparing with the untreated fabric. In future application the nanoparticle loaded fabric
can be used as nose masks, head caps, aprons, curtains, etc.
Keywords: Antibacterial activity, Andrographis paniculata, AATCC and FT-IR.
Bacterial reduction activity of polycotton fabric treated with A. paniculata loaded nanoparticles.
24
CATALYTIC AND BIOLOGICAL ACTIVITIES OF GREEN SILVER NANOPARTICLES SYNTHESIZED N

FROM PLUMERIA ALBA (FRANGIPANI) FLOWER EXTRACT
A
M. Rani, S. Sudha Rani *
Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry N
University, Pondicherry, India. O
Email id: dr.ssrlab@gmail.com, Mob: 09443768726 M
A
This study reports on the green synthesis of silver nanoparticles using Plumeria alba T
(Frangipani) flower extract (FFE) and their biological applications. The formation of frangipani E
silver nanoparticles (FSNPs) was confirmed by UV-visible spectroscopy and they were R
characterized by DLS, SEM/EDX, FTIR, TGA/DSC and XRD. Spherical shaped FSNPs were found to I
be 36.19 nm in size and the EDX pattern of FSNPs confirmed presence of silver. The bioactive A
groups present in FFE that were involved in the formation of FSNPs were identified by FTIR L
analysis. FSNPs exhibited powerful catalytic activity against organic dyes such as 4-nitrophenol S
to 4-aminophenol and degraded them. FSNPs also exhibited potent antioxidant properties as
assessed by various in vitro assays. Antibacterial effect of FSNPs against different pathogenic
bacterial strains was assessed by disc diffusion method and their bacteriostatic effect was
confirmed by growth kinetic studies in E. coli. FSNPs induced cytotoxicity in COLO cells as
indicated by MTT assay with IC50 values of 5.5 μg/ml and 4 μg/ml at 24 and 48 h respectively.
FSNPs mediated cytotoxic effect in COLO cells was associated with loss of membrane integrity,
chromatin condensation and subsequent induction of apoptosis as evidenced by AO/EB staining.
This simple and ecofriendly green synthesis method opens new avenues in nanotechnology for
large scale production of nanomaterials with potential biomedical and industrial applications.
Images of AO/EB staining for apoptosis
25
BIOGENIC SILVER NANOPARTICLES FROM FICUS RELIGIOSA: BIOLOGICAL APPLICATIONS N

N Jayachandra Reddy and S. Sudha Rani*
Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry
A
University, Pondicherry, India. N
Email: dr.ssrlab@gmail.com, Mobile: 09443768726 O
M
A
This study reports on the green synthesis of silver nanoparticles with aqueous Ficus T
religiosa leaf extract (FRLE) at 50 oC, their characterization and in vitro biological activities. The E
synthesized silver nanoparticles (FIAgNPs) were characterized by UV-visible spectroscopy,
R
scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), dynamic
I
light scattering (DLS) particle size analyzer and thermal gravimetric analysis (TGA). The average
A
size of FIAgNPs was found to be 26nm as shown by DLS particle size analyzer. FTIR data indicated
L
that aromatic amine and amide groups of Ficus religiosa are involved in the reduction and
capping of FIAgNPs. Significant level of free radical quenching ability was shown by FRLE and S
FIAgNPs in various in vitro antioxidant assays. FIAgNPs also exhibited moderate antibacterial
activity against Escheria coli, Bacillus subtilis, Pseudomonas fluorescence and Salmonella typhi as
compared to standard antibiotic. The cytotoxic effect of FIAgNPs in four different cell lines- HeLa,
A549, Hep2 and COLO 205 was determined by MTT assay. FIAgNPs induced apoptotic cell death
was shown by acridine orange and ethidium bromide (AO/EB) staining technique. Significantly
increased reactive oxygen species (ROS) levels was detected in FIAgNPs treated cells by DCFDA
staining and spectrofluorimetric analysis. Alteration in mitochondrial membrane potential
(MMP) in FIAgNPs treated cells was indicated by rhodamine 123 staining. PI and DAPI staining
showed nuclear fragmentation or condensation in FIAgNPs treated cells. These data suggests that
green synthesized silver nanoparticles complimented with multifaceted biomedical applications
can serve as better alternatives to nanoparticles synthesized by perilous chemical procedures.
TGA-DSC image of FIAgNPs
26
PREPARATION AND CHARACTERIZATION OF SOLID LIPID NANOPARTICLE FOR CEREBRAL N

MALARIA
A
Roshni Ubnare1, Girdhari Roy1, Vipin Dhote2 and Suman Ramteke1
1
School of Pharmaceutical Sciences, Rajiv Gandhi Proudyogiki N
Vishwavidyalaya, Bhopal. 462036, Madhya Pradesh O
M
2
VNS College of Pharmacy, Bhopal, Madhya Pradesh
Email: sapna1731@rediffmail.com, Mobile: 09302162305
A
T
The objective of the present investigation was to explore the potential of solid lipid
E
nanoparticles containing artemether to accomplish the delivery of drugs to the brain via olfactory
R
delivery system for treatment of cerebral malaria. Nanoparticulate system widely investigated
because of many advantages such as controlled drug release pattern, drug targeting ability, and I
smaller size, improvement of therapeutic efficacy and reduction of toxicity. Artemether-loaded A
solid lipid nanoparticles were prepared by a hot homogenization method followed by solvent L
injection technique. Artemether containing solid lipid nanoparticles composed of soya lecithin S
and poloxamer 407 were characterized for their shape, mean particle size, zeta potential and
encapsulation efficiency and drug release study. These solid lipid nanoparticles were observed
spherical in shape in transmission electron microscopy and optimized size was 211.6 nm (PI <
0.277), with −28mV zeta potential value. The maximum entrapment efficiency was 82% (w/w),
and optimized formulation showed 98% release of artemether. SLN formulations were subjected
to stability study over a period of 30 days. In vivo studied were conducted on wistar rats after
administration of artemether solution orally and artemether containing solid lipid nanoparticles
intranasally, the results of optimized formulation showed value of t1/2 -4.95hours, Cmax -
644.60ng/ml, Tmax -1 hours, Vd - 2.7l/kg, Cl-0.37l.hr/kg and [AUC]0∞ was 3970.54 nghr/ml. In vivo
results revealed that brain: plasma concentration ratio was much higher after intranasal
administration of SLNs of artemether than the control group (oral route). In conclusion, intranasal
administration of SLNs of artemether could provide better protection against cerebral malaria.
Transmission electron microscopy of SLN containing artemether
27
COST-EFFECTIVE MICROFLUIDIC CHANNELS FABRICATION USING SOFT LITHOGRAPHY N

Yammani Venkat Subba Rao, Aravinda Narayanan Raghavan and Meenakshi Viswanathan
Department of Physics, Birla Institute of Technology and Science - Pilani, Hyderabad Campus,
A
Hyderabad – 500078, Telangana N
Email id: meenakshi@hyderabad.bits-pilani.ac.in, Phone: 040-66303535 O
M
Healthcare industry is currently moving towards personalized medicine; in this scenario A
the traditional pharmaceutical and in vitro diagnostic systems future has blurred. More than ever T
quick responsive and accurate tests are required. Microfluidic devices offer innovative solutions E
to increase pharmaceutical research yield with improved data quality, fewer experiments, less
R
reagent consumption, shorter reaction times, better performance, and higher throughput due to
I
parallel processes that lead to reduction of costs. This works reports cost effective fabrication of
A
different micro size/shape channels using Soft lithography. Soft lithography is a collection of
L
fabrication techniques based on pattern design, printing and molding using elastomeric stamps.
Polymethyl methacrylate (PMMA) patterns are used as masters. Microchannels are fabricated S
using the polymer – Poly-Dimethyl Siloxane (PDMS) (Pic a, b): The molten PDMS can readily
convert into solid elastomeric structures by cross linking. Soft lithography includes many
patterning techniques. Among them we have been used Replica Molding (REM) and
Micromolding in Capillaries (MIMIC). Replica Molding (REM) has been used to produce polymeric
structures on a variety of surfaces. Micromolding in capillaries (MIMIC) is based on the
spontaneous filling of fluid into capillaries. Capillaries are formed by placing the patterned side
of PDMS mold into conformal contact with a glassslide there by creating a network of channels.
Microfluidic Channel Images:
“T” Channel Serpentine Channel
28
DESIGN AND EVALUATION OF POLYVINYL-GUM GHATTI SELF-ASSEMBLED NANOSCALE N

PARTICLES FOR ORAL DELIVERY OF SIMVASTATIN
A
Bibek Laha, Leena Kumari, Arpana Kashyap and Sabyasachi Maiti
Department of Pharmaceutics, Gupta College of Technological Sciences, Ashram More, G.T Road, Asansol-713301, N
West Bengal, India O
Email id: sabya245@rediffmail.com, Mob: +919474119931 M
A
T
This work describes the synthesis of a novel gum ghatti-based amphiphilic copolymer and
E
its ability to form nanostructured particles in water. Hydrophobic polyvinyl chain was conjugated
R
in the backbone of hydrophilic gum ghatti by etherification reaction mechanism to impart I
amphiphilic character. Simavastatin, a poorly water soluble LDL-cholesterol lowering drug was A
incorporated by solvent evaporation technique by varying drug: copolymer weight ratio (1:2, 1:4 L
and 1:6).Microscopic analysis of the aqueous copolymer dispersion revealed spherical S
morphological structures. Thus it was suggested that the copolymer self-assembled in water and
formed nanomicellar structures. The size of nanoparticles were characterized by Malvern
Zetasizer Nano ZS 90 apparatus and was found to be in the range of 710.2-802.5nm. The
copolymer dispersion exhibited negative zeta potential values (-20.6-25.3mV) suggesting physical
stability of the dispersion. Even after an observation period of 3months, no signs of aggregation
were evidenteither macro- or micro-scopically. Compared to saturation water solubility of the
drug, about 50 fold increase in drug solubility was observed after copolymer micellization.The
drug loading was found to decrease with increasing amount of the copolymer. Only a small
amount of drug (<10%) released into simulated gastric fluid (pH1.2) and the drug release
extended in simulated intestinal fluid (pH6.8) up to 6h releasing about 95% of the incorporated
drug. The drug release mechanism assumed Fickian diffusion in simulated intestinal fluids.
Preliminary results obtained with the copolymer nanoparticles were encouraging and thus could
be useful as controlled drug delivery carriers.
: Morphological structures of (a) drug-free; and (b) drug-loaded copolymer nanoparticles
29
INVITRO ANTIMICROBIAL EFFICACY OF ZINC DOPED NANOHYDROXYAPATITE AGAINST N

HUMAN PATHOGENS
A
R.Baskar1, G.Devanand Venkatasubbu2 and K.Umamaheswari2
1
Department of Biotechnology, University of Madras, Guindy campus, Chennai-600025, TN N
2
Centre for Biotechnology, Anna University, Chennai-600025, TN O
Emai id: rk_uma@yahoo.com, Mob: 09940326510 M
A
Hydroxyapatite (HA) nanocrystals have attracted much attention for orthopedic and T
dental applications. Long-term invivo performance of HA can be affected by infectious agents. To E
overcome this problem, the use of metal ions in HA is proposed. The objectives of the study was
R
to synthesize Zinc (Zn) doped HA nanocrystals and to determine its antimicrobial efficacy. HA
I
nanocrystals was synthesized by wet chemical precipitation and doped with Zn at 2% and 5%
A
concentration. The morphology, crystallinity and Zn incorporation into HA nanocrystals were
L
studied using High Resolution Transmission Electron Microscopy (HRTEM), X-ray Diffraction
(XRD) and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) respectively. The S
Minimum Inhibitory Concentration (MIC) of Zn-HA nanocrystals were determined against
pathogens by microbroth dilution assay. The synthesized Zn-HA nanocrystals exhibited a uniform
morphology with size of 10-20nm and crystalline in nature. The weight percentage of zinc in 2%
and 5% Zn-HA nanocrystals were found to be 0.4990 and 1.3740 respectively. The Zn-HA
nanocrystals showed inhibition against E. coli, P. aeruginosa, K. pneumonia, S. aureus and C.
albicans at a concentration between 15 - 500 µg/mL. The results of the present study shows that
5% Zn-HA nanocrystals shows good antimicrobial activity than 2% Zn-HA nanocrystals
Furthermore, in vivo studies on Zn-HA will give better understanding about the long term
performance of HA.
S. No Organism Tested MIC (µg/mL) of

2% Zn-HA 5% Zn-HA
1. Escherichia coli 427.8 453.0
2. Pseudomonas aeroginosa 500.0 468.0
3. Klebsiella pneumonia 250.0 218.7
4. Staphylococcus aureus 343.7 234.3
5. Candida albicans 66.4 16.5
MIC of Zn-HA nanocrystals
30
ABSORBANCE, TRANSMITTANCE AND BANDGAP STUDIES OF COMPLEXING AGENT N

TRIETHANAOLAMAINE ADDED IRON DOPED ZINC SULFIDE THIN FILMS BY SILAR METHOD
A
V. Helen *, K Manikandan, P Mani, M.Shahul hameed, & J Joseph Prince
Department of Physics, Anna University: BIT Campus, Tiruchirappalli 620 024 N
O
Iron doped Zinc Sulfide thin films are deposited on microscopic glass substrate by M
chemical technique called successive ionic layer adsorption and reaction (SILAR) method at room A
temperature. The technique involves multiple dipping of the substrate in an aqueous solution of T
Zin nitrate, ferrous nitrate, sodium sulphide and in deionised water. The aim of this study is to E
establish the optical properties (Absorbance, Transmittance and Band gap) of Iron doped Zinc R
Sulfide thin films by UV-VIS spectrometer and the influence of molar concentration was studied I
to determine the optimum condition for deposition process. The high absorbance of the films A
made them good materials for conversion of solar energy. The transmittance decreases with L
increasing molar concentration. The band gap energy decreases when molar concentration S
increases.
31
PHARMACOKINETIC STUDY OF NELFINAVIR NANOCRYSTALS N

Dasari Naga Naresh1, Usha Y Nayak1, Yogendra Nayak2*
1 Department ofPharmaceutics,Manipal College of Pharmaceutical Sciences, A
Manipal University,
2
Manipal,Karnataka, India-576104, Department of Pharmacology,Manipal College of Pharmaceutical Sciences, N
Manipal University, Manipal, Karnataka, India-576104 O
Email id: yogendranayak@gmail.com; yogendra.nayak@manipal.edu; Mob: 9448154003 M
A
Nelfinavir, an antiviral drug possess very low intrinsic dissolution rate, hence, it has T
dissolution rate limited absorption leading to poor pharmacokinetic profile. Therefore E
nelfinavirnanocrystals were prepared by ball milling method and its pharmacokinetic behavior
R
was studied in Wistar rats. Nelfinavir was dispersed in poloxamer 407solution (0.25% to 1% w/v)
I
and subjected for size reduction. The particle size and zeta potential of optimized formulation
A
was found to be 740.12 ± 79.21 nm and 23.31 ± 1.10mV, respectively with PDI of 0.20 ± 0.07. The
L
overnight fasted rats were divided into 2 groups (n = 6) and treated orally (20 mg/kg) with
optimized crystals and pure drug. Nelfinavirnanocrystals were dispersed in 1 mL of 0.25% (w/v) S
carboxyl methyl cellulose aqueous solution. Rat blood samples were collected by tail vein at
predetermined time intervals.Liquid – liquid extraction method by methyl tert-butyl ether was
used to extract the drug from blood and analysed by HPLC. The pharmacokinetic parameters
were calculated using non-compartmental analysis using WinNonLin software. The plasma
concentration of drug against time along with optimized nanocrystals is shown in Figure. In vivo
study revealed lower Tmax and higher Cmaxand AUC0-t indicating improvement in bioavailability for
nelfinavirnanocrystals. It might be due to increase in dissolution velocity of nanocrystals which
increases rate of absorption.Thussignificant increase in solubility and bioavailability of
nelfinavirmesylate was observed by formulating it as nanocrystals.
32
FORMULATION OF OLMESARTAN MEDOXOMIL NANOSUSPENSION: IN- VITRO N

CHARACTERIZATION AND EX-VIVO PERMEATION
A
R.Gunjal, T.Yadav, S.Dhat
Email id: rahulkumar.gunjal@gmail.com, shalakapd@gmail.com; O
Mobile: +919970209669, +919823270454 M
A
Olmesartan medoxomil (OLM), an angiotensin-II receptor antagonist has poor aqueous T
solubility (7.75 μg/ml) and only 26% bioavailability in humans. In the present research work E
attempt has been made to enhance the bioavailability of OLM by formulating OLM
R
nanosuspension using high pressure homogenization and Poloxamer 407 as a stabilizer.
I
Optimization using 32 factorial design confirmed that increase in poloxamer concentration (0.4%
A
- 1.2% w/v) and homogenization pressure (400 - 1200 bars), decreases the particle size. The
L
stability of the optimized nanosuspension (1.2% w/v Poloxamer 407 and 1200 bar) having
zetapotential of -12.7 µV was enhanced by lyophilization using 8% mannitol as a cryoprotectant. S
Particle size and polydispersibility index of OLM lyophilized nanoparticles (NPs) was 392.14nm
and 0.094 respectively. Lyophilized NPs were found to increase the saturation solubility by 15.29
folds (115.65 µg/ml) in distilled water and 17.72 folds (2080.43 µg/ml) in phosphate buffer pH
6.8. In-vitro release studies in phosphate buffer pH 6.8 showed 96.79 ± 0.027 % OLM release from
lyophilized NPs, whereas pure OLM showed only 19.81 ± 0.023 % release in15 min. In vitro
diffusion studies across dialysis membrane showed 75.09 ± 0.056 % OLM diffusion in contrast to
36.18 ± 0.035 % diffusion from pure OLM in 6 hrs. Ex vivo drug permeation and the corresponding
flux of lyophilized NPs was increased by 1.99 and 2.56 folds respectively as compared to pure
OLM. Thus in vitro release, in vitro diffusion and ex vivo studies confirm the potential of the
formulated nanoparticulate drug delivery of OLM for bioavailability enhancement.
Code Permeability coefficient Flux (J)

(P) (mg.cm/min) (mg/cm2.min)
OLM 0.009 0.036
OLM nanosuspension 0.022 0.086
Lyophilized NPs 0.023 0.092
marketed tablet 0.009 0.036
Permeability coefficient of OLM across rat intestinal membrane and flux values
33
FORMULATION, OPTIMIZATION AND CHARACTERIZATION OF SATRANIDAZOLE LOADED PLGA N

NANOPARTICLES
A
K.Singh, M Rajput, S.Dhat
Email id: skanwar43@gmail.com, shalakapd@gmail.com; O
Mob: +919021477377, +919823270454 M
A
Satranidazole (STZ), a 5-nitroimidazole is more active than metronidazole against
T
anaerobic bacteria causing periodontitis. In comparison to systemic delivery, a localized delivery
E
of STZ in periodontal pocket can provide better therapeutic benefits at a reduced dose. The
R
objective of the present study was to formulate poly (d,l-lactide-co-glycolide) (PLGA) loaded STZ
nanoparticles by emulsion solvent evaporation technique. A 23 full factorial design was used to I
study the effect of varying concentration of PLGA (30-60 mg), poly vinyl alcohol (PVA, 0.4- 0.8%) A
and sonication time (15-25 min) on the particle size and entrapment efficiency. The nanoparticles L
were lyophilized using 6% mannitol. Based on the experimental design and the 3D response S
surface plots, an optimized formulation was selected. The particle size and polydispersibility
index (PDI) of optimized lyophilized nanoparticles was found to be 377.15 and 0.233 respectively.
The entrapment efficiency was found to be 95.78%. A positive zeta potential (3.15mV) of
lyophilized nanoparticles was promising for achieving therapeutic effect of the formulation. In
vitro release studies of lyophilized nanoparticles containing 30 mg STZ in McIlvaine buffer (pH
6.6) showed release of STZ from nanoparticulate formulation in two phases; an initial burst
release (55.27%) in the first four hours followed by slow release of the drug (96.3%) over period
of 24 h. MIC and MBC studies confirmed the effectiveness of nanoparticulate formulation at a
one tenth dose of STZ as compared to conventional dose of 300mg.
3D Response graph for particle size encapsulation efficiency
34
EUDRAGIT® RL 100 LOADED OFLOXACIN NANOPARTICLES: FORMULATION BY DESIGN N

D.Nagane, M.Rajput, S.Dhat
Department of Pharmaceutics, S.T.E.S.’s Sinhgad Institute of Pharmacy, Pune, Maharashtra, India
A
Email id: dnagane@ymail.com, shalakapd@gmail.com; N
Mob: +919922426925, +919823270454 O
M
In the present work a three factor, two level full factorial design was employed for A
optimization of Eudragit®RL 100 loaded ofloxacin nanoparticles by emulsion solvent T
evapopration technique NEMRODW software (LPRAI SARL, Marseille, France) was used to E
determine the optimum level of Eudragit®RL100 (X1), PVA (X2) and sonication time (X3) that can
R
provide minimum particle size (Y1) and maximum entrapment efficiency (Y2) of ofloxacin.
I
Polynomial models together with interaction terms were generated for both the response
A
variables (Y1 and Y2) by means of multiple linear regression analysis. Graphical analysis of effects
L
by Lenth’s method and Bayesian analysis of coefficients enabled identification of variables
influencing the selected responses. Based on the experimental design, Eudragit RL100 (50mg), S
PVA (0.66%) and sonication time (25min) was found to be optimum and showed particle size 219
± 0.46 nm, PDI 0.233 and entrapment efficiency of 96 %. The nanoparticle dispersion was
lyophilized using 1% trehalose as lyoprotectant. The zeta potential (+29.80mV) is effective in
achieving the desired stability and therapeutic effect. In vitro release studies of lyophilized
nanoparticles in McIlvaine buffer (pH6.6) showed an initial burst release (38.23%) in the first four
hours followed by slow release of remaining drug (93.98%) over period of 52 h. In contrast to this
pure OFLX showed (84.60%) release in 4 h. briefly, optimization using factorial design was found
be effective tool in the formulation of ofloxacin nanoparticles.
Graphical representation of effect of selected factors (X) using Length’s methods; (a) and
Bayesian analysis (b)
35
DICLOFENAC SODIUM NANOSUSPENSION: AN APPROACH TO IMPROVE ANTI- N

INFLAMMATORY THERAPY
A
S. LakshmanaPrabu, S.P. Sharavanan, A. Shanmugarathinam, K. Ruckmani, S. Aravindan*,
A. Bhuvaneswari, V. Manikandan N
Dept. of Pharmaceutical Tech., Bharathidasan Institute of Technology, Anna University, Tiruchirappalli – 620 024. O
M
Drugs with low aqueous solubility and high permeability present a high proportion of all A
drugs. Poorly water-soluble compounds are frequently abandoned early in discovery and are T
difficult to be developed into drug products by using conventional formulation techniques. In this E
study, we present an approach of nanosizing a drug/polymeric complex to increase both R
solubility and dissolution rate of poorly water soluble drug diclofenac sodium. Polymeric I
nanosuspensions were prepared by nanoprecipitation method by using biodegradable polymer
A
loaded with diclofenac sodium (DS), with the aim to improve anti-inflammatory therapy. The
L
prepared formulation was evaluated for drug excipients compatibility study, polydispersity index,
S
particle size analysis, surface morphology, zeta potential, drug release profile and stability. These
study results indicate a suitable formulation procedure for preparation of nanosized poorly water
soluble drug with significantly improved in vitro dissolution rate, and thus possibly enhanced fast
onset of therapeutic drug effect.
36
REDOX ENVIRONMENT CLEAVABLE POLYMERIC NANOPARTICLES FOR DRUG DELIVERY N

M.Gover Antoniraj, C.Senthil Kumar, S.AngelineTisha* and K.Ruckmani
Department of Pharmaceutical Technology, Bharathidasan Institute of Technology, Anna University,
A
Tiruchirappalli-620024 N
O
Chitosan and Methoxy polyethylene glycol (mPEG) have been broadly studied as delivery M
carrier for drugs and various biomolecules such as DNA and siRNAs. Conjugation of chitosan with A
mPEG via disulphide linkage was, therefore, anticipated to produce reduction sensitive drug T
delivery at disease site. In this study, thiolated chitosan and mPEG-SH was synthesised and E
conjugated through disulphide linkage CH-SS-mPEG by oxidation reaction. Synthesised polymer R
characterized by FTIR analysis. CH-SS-mPEG nanoparticles (CNs) were prepared by ionic gelation I
method using Sodium tripolyphosphate (STPP) as gelation agent. Size of the particles and surface A
charge value was evaluated for formulated polymeric nanoparticles. An in-vitro drug release
L
study was carried out for disulphide linked polymeric nanoparticles with and without reducing
S
agent glutathione (GSH). A drug release study shows that the rapid release at reduction medium
indicates glutathione induced drug release by the reduction of disulphide. In conclusion,
disulphide conjugated CH-SS-mPEG was successfully synthesized, and prompt drug released in
the presence high concentration of GSH.
37
SYNTHESES AND CHARACTERIZATION OF PAMAM DENDRIMER AND ITS APPLICATION FOR N

REMOVAL OF HEAVY METALS
A
E.Gomathi1, P.Rajesh Prasanna2 & P. Selvamani3
1
Department of Petrochemical Technology 2Department of Civil Engineering 3Department of Pharmaceutical N
Technology, BIT Campus - Anna University Tiruchirappalli – 620 024, Tamil Nadu, India O
M
1
gomathipetro@gmail.com 2 rajeshprasanna71@yahoo.com
A
The objective of the present study is to synthesize silica based PAMAM dendrimer with
T
ester and amino groups at the outer surface. The synthesis of dendrimer is usually carried out in
E
two ways namely convergent technique and divergent technique. PAMAM is normally
R
synthesized by divergent methods starting from ammonia or ethylenediamine initiator core
reagents. Amine terminated PAMAM dendrimers exhibit and high affinity for adsorption of metal I
ions to their surface via co ordination to the amine or the acid functionality. The structures were A
characterized by FTIR, SEM and AAS. FTIR methods were employed to monitor amidation reaction L
in order to judge the optimum reaction time. The experiments showed that both ester- and S
amino-terminated dendrimer-like polyamidoamine (PAMAM) grafted silica-gel exhibited better
adsorption capabilities for base metal ions, Cr, Hg. It was shown that the adsorption data of the
composite could be fitted using the Langmuir equation with a maximum adsorption capacity.
Keywords: Silica-gel, Polyamidoamine-typed hyper branched polymer Preparation, Adsorption,
Metal Ions.
SEM image of G1 PAMAM Dendrimer SEM image report of G2 PAMAM dendrimer
38
IN-VITRO CYTOTOXICITY STUDIES OF NICKEL OXIDE NANOPARTICLES ON CANCER CELLS USING N

MTT ASSAY
A
K.Perumal Raj*1, Dr.V.Thangaraj2, Dr.A.P.Uthirakumar3, P.Sivakarthik4
1. Assistant Professor in Chemistry, VSB Engineering College, Karur. 2. Assistant Professor in Chemistry, Anna N
University (BIT Campus), Tiruchirappali. 3. Assistant Professor in Chemistry, SONA College of Technology, Salem. O
4. Assistant Professor in Chemistry, University College of Engineering, Panruti. M
Email id: perumalrajk@gmail.com
A
T
The aim of this study to observe the cytotoxicity of synthesized Nickel oxide nanoparticles
E
on human cancer cell lines using MTT assay. The precursor for the Nickel oxide nanoparticles
R
were synthesized using nickel sulphate by organic solvent assisted solution method. The
synthesized NiO nanoparticles (<100 nm) were characterized by X-ray diffraction analysis, I
Scanning Electron microscopy analysis, UV and IR spectral techniques. The characterization A
studies revealed that growth of the nickel oxide nanoparticles were regularized and perfect. The L
synthesized nickel oxide nanoparticles were subjected to in-vitro cytotoxicity studies against S
various human cancer and normal cell lines using MTT assay model. All the synthesized Nickel
oxide nanoparticles showed moderate to significant cytotoxic activity against the tested cell lines.
IC50 (µg/ml)*
Cell lines studied
NiO-Ace Cisplatin
NHDF (Normal Human Dermal Fibroblasts) 27.95 4.45
HeLa (Human Cervical Cancer Cells) 15.28 1.15
MCF7 (Human Breast Cancer Cells) 11.46 1.6
HepG2 (Human Liver Cancer cells) 27.72 2.87
A-549 (Human Lung Cancer Cells) 28.39 3.12
39
TIN OXIDE NANOPARTICLE CATALYZED ONE-POT SYNTHESIS OF 3, 4 DIHYDROPYRIMIDINONES N

P.Sivakarthik1, V.Thangaraj2
1. Assistant Professor, Department of Chemistry, University College of Engineering, Panruti
A
2. Assistant Professor, Department of Chemistry, Anna University BIT Campus N
Email id: psivakarthick@yahoo.com, thangamraj@gmail.com O
M
Tin oxide nano particle is an efficient, inexpensive, and readily available catalyst for the A
three components one-pot condensation reaction of an aldehyde, urea/thiourea and 1, 3- T
dicarbonyl compounds to afford the corresponding dihydropyrimidinones in high yield. This E
technique is non-polluting method and does not employ any toxic reagents, quantifying it as a
R
green approach to this cyclocondensation reaction. Compared with the classical Biginelli reaction
I
conditions, this new method has the advantage of excellent yield and shorter reaction time. All
A
the compounds have been characterized by Elemental analysis, IR, 1H NMR and 13C NMR Spectra.
L
Keywords: Nanoparticles, Dihydropyrimidinones, Biginelli reaction, cyclocondensation, NMR S
spectra
R 4'
5' 3'
6' 2'
O 1'
4
5 3
R1 NH
2
6 X
H3C N1
H
The Structure of 3, 4 Dihydropyrimidinones
40
ENHANCED ORAL BIOAVAILABILITY OF NARINGENIN POLYMERIC NANOPARTICLES: N

FORMULATION, CHARACTERIZATION AND IN VIVO STUDIES
A
P. Suseelaa, K. Premkumarb, S.D. Saraswathya,*
a
Molecular Pathology and Toxicology Laboratory, Department of Biomedical Science, School of Basic Medical N
Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India O
M
b
Cancer Genetics and Nanomedicine Laboratory, Department of Biomedical Science, School of Basic Medical
Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India
A
T
Flavonoids are natural products widely distributed in plant kingdom and currently
E
consumed in large amounts in the daily diet. Naringenin (NRG) is a flavonoid specific to citrus
R
fruits and possesses anti-inflammatory, anticarcinogenic, and antitumor effects. To improve the
solubility and prolong its duration in body system, the present study was designed to formulate, I
characterize and evaluate its pharmacokinetic profile in NRG encapsulated polymeric A
nanoparticles (PNPs). NRG encapsulated PNPs were formulated by nanoprecipitation method in L
ten different batches and named as NF1, NF2 ………..NF10 respectively. The formulated S
nanoparticles were characterized by Dynamic light scattering (DLS), Zeta potential (ζ), Scanning
Electron Microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR). The in vitro drug
encapsulation efficiency, drug release and in vivo pharmacokinetic profile and toxicity studies
were performed with the formulated nanoparticles. Among ten different batches studied, the
NF10 batch showed lowest mean particle size and highest zeta potential was found to be 78 ± 12
nm and -34 ± 1.64 respectively. The characteristic bands of NRG in FTIR were either shifted or
reduced in the spectrum of PNPs. Scanning electron microscopy of naringenin nanoparticles
morphology revealed that spherical in shape. In vitro drug release study showed that PNPs was
capable of releasing the drug in sustained manner. Enhanced pharmacokinetic parameters were
seen in PNPs as compared to free NRG. From our results we thus conclude that PNPs prepared in
NF10 batch was the most effective formulation for enhanced oral bioavailability which can be
used for further studies to identify its biomedical applications.
130
PBS
110
SGF
cumulative % release
90 SIF
70
50
30
10
0 2 4 6 8 10 12
Time (hr)
In-vitro release profile of PNPs in various releasing media
41
SYNTHESIS OF GSH & FA CONJUGATED CHITOSAN FUNCTIONALIZED GOLD NANOSPHERES: N

PHYSICOCHEMICAL PROPERTIES AND APPLICATIONS IN CANCER THERAPY
A
Kalpana Haria, Hemamalini Vedagirib, and Premkumar Kumpatia*
a
Cancer Genetics and Nanomedicine Laboratory, Department of Biomedical Science, Bharathidasan University, N
Tiruchirappalli-620024, Tamil Nadu, India. O
M
b
Department of Biotechnology, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu
A
Gold nanoparticles (AuNPs) have been widely used in various biomedical applications due T
to their unique size tunable optical electronic properties and enhanced permeation and retention E
effect. In addition, the external characteristics of AuNPs possess a strong surface chemistry that
R
aids them suitable for the attachment of biomolecules and drugs. The conjugation of
I
biomolecules with AuNPs provides the high affinity and bioavailability to target the various
A
diseases. In this research we have synthesized and characterized stable monodispersive gold
L
nanospheres (AuNS) by environmental benign green method using chitosan biopolymer via
simple wet chemical reduction. The synthesized AuNS were characterized by assessing their size, S
zeta potential, and surface characteristics. Folic acid was then conjugated by using activated folic
acid via terminal amino groups of the chitosan, and were purified from unreacted products.
Conjugation of glutathione (GSH) was successfully carried out through thiol chemistry. The
specific interaction between the folic acid / GSH with AuNS was evaluated by surface plasmon
resonance, which confirmed specific binding of the folate (FA) and GSH on the surface of AuNS.
This interaction did not occur with nonconjugated nanoparticles used as control. From the results
it has been concluded that chitosan reduced AuNS possess suitable surface and functional groups
to conjugate biomolecules for the targeted therapy. The target specificity of AuNS coated with
GSH and Folic acid was tested using breast cancer cell lines and both cell types exhibit significant
uptake of GSH &FA conjugated AuNS. Thus, FA and GSH conjugated AuNS nanoparticles represent
a potential new drug carrier for tumor cell-selective targeting.
42
GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM LEAF EXTRACTS OF BREYNIA VITIS- N

IDAEA
A
T.P. Rajesh
Assistant Professor Department of Biotechnology University College of Engineering Bharathidasan Institute of N
Technology CampusTrichirappalli – 620024 India Mobile: +91-9698376901 Email: tprajesh2009@gmail.com O
M
The development of the biological synthesis of nanoparticles using plant extracts plays an A
important role in the field of nanotechnology as it is environmentally friendly and does not T
involve any harmful chemicals. In this study, the synthesis of silver nanoparticles using the leaves E
extract of Breynia vitis-idaea is reported. The synthesized nanoparticles were characterized using R
UV-visible spectroscopy, X-ray diffraction (XRD) and Particle size analyzer. The XRD analysis I
shows that the synthesized silver nanoparticles are of face-centered cubic structure. Well- A
dispersed silver nanoparticles with an approximate size of 50-70 nm were observed in the particle
L
size analysis studies. The application of the synthesized silver nanoparticles can be in the field of
S
cosmetics, food packaging industry, medicine for antimicrobial fabrics or surgical knives, sterile
gloves, optics as electrochromic devices, and electronics as conductive connections etc.
43
PREPARATION AND CHARACTERIZATION OF PLUMBAGIN LOADED BOVINE SERUM ALBUMIN N

NANOPARTICLES FOR DRUG DELIVERY APPLICATIONS
A
Bhuvana Meenal.S, Vengadeswari.V and Ronaldo Anuf.A*
Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar N
Email: ronaldoanuf@gmail.com O
M
Plumbagin is a quinoid constituent isolated from Plumbago rosea and has a broad A
spectrum anticancer activity against diverse models such as prostate, lung, cervical as well as T
melanoma. However the wide spread application of plumbagin has been limited by its poor E
bioavailiability. Nanoparticles are submicron and sub-cellular in size and possess versatile R
advantages such as increased surface area and reactivity, increased gastric residence time and I
improved solubility in both aqueous and organic phases. Due to their size, nanoparticles have the A
advantage of reaching inaccessible sites in the body by escaping phagocytosis and entering tiny
L
capillaries. Sustained release of the drug from the nanoparticles maintains the therapeutic
S
concentration for long durations. Bovine serum albumin (BSA) is an attractive macromolecular
carrier and widely used to prepare nanospheres and nanocapsules, due to its availability in pure
form, its biodegradability, nontoxicity and non immmunogenicity. These protein-based
nanoparticles due to there defined primary structure offer various possibilities for surface
modification and covalent drug attachment. In the present study, Plumbagin loaded BSA
nanoparticles was prepared by desolvation method. Here in, we have investigated the effect of
BSA and plumbagin composition on drug encapsulation, to elucidate their influence on particle
size, zeta potential, drug loading and encapsulation efficiency of resulting nanoparticles. Varying
this parameter, drug loaded nanoparticles was synthesized in a size range of 100- 300 nm with a
drug encapsulation efficiency of 60 – 85 %. In the near future, the results of these studies will
help to investigate the in vitro and in vivo effect of these drug loaded nanoparticles on diverse
cancer models
44
BIOMIMETIC PRODUCTION AND CYTOTOXIC EFFECT OF SILVER NANOPARTICLES FROM FUNGI N

S. Akila and Anima Nanda
Department of Biomedical Engineering, Sathyabama University,
A
Rajiv Gandhi Salai, Chennai - 600119, India. N
*Email: akila.hai@gmail.com, Mobile: 9176295082 O
M
Nanosized materials have been an important subject in basic and applied sciences A
because of their unique optical, thermal, electrical, chemical, and physical properties that are T
due to a combination of the large proportion of high-energy surface atoms compared to the bulk E
solid. AgNPs have been used extensively as anti-bacterial agents in the health industry, food
R
storage, textile coatings and a number of environmental applications. The microbial-mediated
I
biological synthesis of metallic nanoparticles has recently been recognized as a promising source
A
for mining nanomaterials. The biological approach to synthesize metal nanoparticles is an
L
important aspect of current nanotechnology research. The ever-increasing antibiotic resistance
in pathogenic and opportunistic microorganisms is a major threat to the health care industry. The S
research work deals with the synthesis of silver nanoparticles from fungi Penicillium.
Biosynthesized silver nanoparticles were characterized by means of several analytical techniques
including a UV-Visible spectrophotometer, Fourier transform infrared spectroscopy, X-ray
diffraction pattern analysis, and scanning electron microscopy techniques. An evaluation of the
antimicrobial activity of silver nanoparticles (AgNPs) was carried out against clinically important
pathogenic microorganisms. The metal nanoparticles were also evaluated for their combined
effects with antibiotics against the clinical pathogens. The antibacterial activities of the
antibiotics increased in the presence of the biologically synthesized AgNPs against the clinically
important pathogens.
Keywords: Biosynthesis of silver nanoparticles, Penicillium sps, characterization, cytotoxic activity
45
DEVELOPMENT OF ANTIMICROBIAL DIAPERS AND WIPES USING THE COMBINATORIAL N

HERBAL EXTRACT LOADED NANOPARTICLES COATED ON THE PLASMA PRE TREATED BAMBOO A
FABRICS. N
Kongarasi.K, Rajendran.R, Radhai.R*, Rajalakshmi.V O
PG and Research Department of Microbiology, PSG College of Arts and Science, Coimbatore. M
E mail: radhai.research@gmail.com Mobile: 9994552517 A
T
Diaper rashes are a common condition among babies by using artificial diapers and wipes
E
because of its chemical preservatives. Treatment for this condition could be possibly done by
R
using traditional herbs and innovative textiles. The present study was carried out for the
I
treatment of diaper rashes using combinatorial herbal extract loaded nanoparticles coated
bamboo fabric pre treated with plasma, which further was developed as diapers and wipes. Aloe A
vera and Rosa damascene powder were subjected to different solvent extracts and their L
antimicrobial activities against test pathogens were studied. The bamboo fabric was exposed to S
DC air plasma at optimized conditions to increase the hydrophilicity of the fabric. The
nanoparticle loaded with combinatorial herbal extract was synthesized by cation induced
controlled gelification method. The nanoparticles were characterized physically and chemically using
DLS, FTIR and GC-MS respectively. Synthesized nanoparticles were further coated on the plasma
treated bamboo fabric using pad dry cure method. The antimicrobial activity of the coated fabric
was then studied using AATCC 147, AATTCC 100 and AATCC 30. The herb loaded nanoparticles coated
bamboo fabric pre treated with plasma showed a better activity against the test organisms associated
with diaper rash. Effectiveness of the antimicrobial finished fabrics was assessed using wash
durability analysis which proved that the antimicrobial activity of the herb loaded nanoparticles
coated fabric sustained till 30 washes. The results showed that the formulated bamboo fabrics
were effective against diaper rashes and it also proves to be antimicrobial. These kind of
hydrophilic fabric coated with nanoparticle loaded with herbal extract would be a better
alternative for baby diapers and wipes.
Keywords: Bamboo fabric, Plasma, Antimicrobial, diapers and wipes.
Size of the nanoparticles loaded with Antibacterial assessment of the treated fabric against
combinatorial herbal extract S.marcescens and S.aureus
46
SYNTHESIS AND CHARACTERIZATION OF MICHELIA CHAMPACA LEAF EXTRACT LOADED N

NANOPARTICLES FOR THE DEVELOPMENT OF ANTIMICROBIAL TEXTILES
A
Tharani R, Rajendran R, Radhai R*, Rajalakshmi V, Savitha R
PG and Research Department of Microbiology, PSG College of Arts and Science, Coimbatore. N
Email id:radhai.research@gmail.com O
M
The protective aspects of textile have provided the most textile ground for innovative A
developments. Most textile materials currently used in hospitals are conducive to cross-infection T
or transmission of diseases caused by micro-organisms. Textiles for medical and hygienic use E
have become important areas in the textile industry. The recent advances in modern biology have R
unboltened the door for nanotechnology and nanosciences. The present study focused on I
development of antimicrobial cotton fabric using herbal extract loaded nanoparticles. Michelia A
champaca powder was subjected to different solvent extract and their antimicrobial activities
L
against test pathogens were studied. The methanolic herbal extract showed higher activity, when
S
compared to other solvents and it was used for further study. The nanoparticle loaded with
herbal extract was synthesized by cation induced controlled gelification method. The
nanoparticles were characterized using FTIR and TEM analysis. Synthesized nanoparticles were
further coated on the cotton fabric was then studied for antimicrobial assessment using standard
methods - AATCC 147, AATCC 100 and AATCC 30. The wash durability analysis of the herbal
extract loaded nanoparticles coated fabrics showed better activity till 30 washes. The result of
the present study confirmed that the herbal loaded nanoparticles coated fabric showed the
better antimicrobial activity against the test pathogens. This kind of eco friendly antimicrobial
fabric will protect the users from microbial infection thereby reducing the burden through
fomites.
Keywords: Michelia champaca, Nanoparticles, AATCC, Antimicrobial fabrics.
TEM micrograph showing M. champaca leaf extract encapsulated

nanoparticles
47
EFFECT OF COMPLEXING AGENT TEA: THE STRUCTURAL, MORPHOLOGICAL, TOPOGRAPHICAL N

AND OPTICAL PROPERTIES OF FexSx NANO THIN FILMS DEPOSITED BY SILAR TECHNIQUE
A
K. Manikandan *, P Mani, V. Helen, S.Syed Zahirullah, & J Joseph Prince
Department of Physics, Anna University: BIT Campus, Tiruchirappalli 620 024 N
E-mail: 1984manikandan@gmail.com O
M
Iron sulfide thin films (FexSx) (x = 0.05 M, 0.10 M, 0.15 M, 0.20 M and 0.25 M) were A
deposited by SILAR method from equimolar and equivolume aqueous solutions of ferrous nitrate T
and sodium sulfide with the addition of complexing agent TEA. The structural, morphological and E
optical characteristics of the films were derived from X-ray diffraction (XRD), scanning electron
R
microscopy (SEM), atomic force microscopy (AFM) and UV–vis spectral techniques. The mixed
I
characteristics (crystalline and amorphous) of the deposited films and the increasing crystalline
A
qualities with the concentrations were understood from the XRD analysis. The grain sizes and
L
roughness of the films were decreases with the increasing concentration and also at the higher
concentration films are shown by the same images presence of hexagonal like crystallite S
structure. The influence of complexing agent TEA on the surface roughness and morphological
properties are confirmed by the atomic force microscope (AFM) results. The effect of increasing
substrate concentration on the absorption and transmission measurements and its impact on the
optical band-gap energy were enumerated from the UV–vis analysis.
48
DICLOFENAC SODIUM NANOSUSPENSION: AN APPROACH TO IMPROVE ANTI- N

INFLAMMATORY THERAPY
A
S. LakshmanaPrabu, S.P. Sharavanan, A. Shanmugarathinam, K. Ruckmani, S. Aravindan*,
A. Bhuvaneswari, V. Manikandan N
Department of Pharmaceutical Technology, Bharathidasan Institute of Technology Campus, Anna University, O
Tiruchirappalli – 620 024, Tamil Nadu. M
A
Recently drugs with low aqueous solubility and high permeability present a high T
proportion of all drugs. Poorly water-soluble compounds are frequently abandoned early in E
discovery and are difficult to be developed into drug products by using conventional formulation R
techniques. In this study, we present an approach of nanosizing a drug/polymeric complex to I
increase both solubility and dissolution rate of poorly water soluble drug diclofenac sodium.
A
Polymeric nanosuspensions were prepared by nanoprecipitation method by using biodegradable
L
polymer loaded with diclofenac sodium (DS), with the aim to improve anti-inflammatory therapy.
S
The prepared formulation was evaluated for drug excipients compatibility study, polydispersity
index, particle size analysis, surface morphology, zeta potential, drug release profile and stability.
These study results indicate a suitable formulation procedure for preparation of nanosized poorly
water soluble drug with significantly improved in-vitro dissolution rate, and thus possibly
enhanced fast onset of therapeutic drug effect.
49
FORMULATION OF SOAP AND CREAM FROM BIOSYNTHESISED SILVER NANOPARTICLES OF N

ADATHODA VASICA NEES. LEAF EXTRACT
A
H. Linda Jeeva Kumari1,2, R. Krishnamoorthy2, S. Sonia3, M. Sivakumar3 and K. Ruckmani1,2*
1
National Facility for Drug Development for Academia, Pharmaceutical and Allied Industries, Bharathidasan N
Institute of Technology, Anna University, Tiruchirappalli 620 024, Department of Pharmaceutical Technology, O
Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620 024,3Division of Nanoscience and M
Technology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620 024
A
Email id: hodpharma@gmail.com, Mob: +91 98424 84568
T
E
Green synthesis of silver nanoparticles have gained much attention in the present days
R
because of their efficacy, low toxicity, easy and less expensive production compared to that of
I
the conventional chemical synthesis. In the present study, we focus to adopt an eco-friendly
A
method for the biosynthesis of silver nanoparticles (AgNPs) using aqueous Adhatoda vasica leaf L
extract with its phytoconstituents acting as reducing and capping agents for the reduction of S
silver nitrate into pure silver ions. Various molar concentrations (1mM, 2 mM, 3 mM and 4 mM)
of silver nitrate solution were prepared and 1 ml of each were added with 100 µl, 200 µl, 300 µl,
400 µl and 500 µl of plant extract at room temperature and observed for 24 h. The visible colour
change from yellow to reddish brown indicates the formation of AgNPs. Optimisation was done
by physical observation such as stability of the synthesised nanoparticles without agglomerating
and obtaining the Surface Plasmon Resonance peaks within the expected range of AgNPs by UV-
Visible Spectroscopy. Its pH was also determined (pH 5.2). This was further characterized by
Fourier Transform Infra-Red spectroscopy (FT-IR), Atomic Force Microscopy (AFM), High
Resolution Transmission Electron Microscopy (HR-TEM) and X-ray Diffraction (XRD).
Furthermore, these nanoparticles can be used in various formulations to exploit their goodness
for human use. Hence, the biosynthesised AgNPs using Adathoda vasica leaf extract was used for
anti-bacterial soap and cream formulations. The antimicrobial activities of the AgNPs, Soap and
Cream were done against various clinical pathogens to prove their efficacy alongside standard
antibiotic, commercial antimicrobial soap and a commercial antimicrobial cream respectively.
Hence, the obtained formulations are a boon to the pharmaceutical industries.
3D AFM image of AgNPs Soap Cream
50
OPTIMIZATION STUDIES ON BIOINSPIRED GREEN SYNTHESIS OF SILVER NANOPARTICLES N

USING CLITORIA TERNATEA
A
N. John Sushma1*, G. Swathi1, D. Prathyusha1and B. Deva Prasad Raju2
1
Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati – 517 502 N
2
Department of Future Studies, Sri Venkateswara University, Tirupati – 517 502 O
M
Plant mediated synthesis process was more advantageous over the chemical mediated A
synthesis method because it was more environmental favorable, less time consuming, large T
scaled up and low cost. Keeping this in mind, the present investigation has been taken up with E
the biological synthesis of Silver nanoparticles (AgNps) using whole plant methanolic extract of R
Clitoria ternatea for the bioreduction of silver ions to nanoparticles. The bioreduction process I
was carried out to study the various factors affecting the nanoparticles synthesis via changing the A
silver ion concentration, pH and temperature. 5 mM silver ion concentration, pH 9 and
L
temperature 50 ºC is more favorable for maximum production of silver nanoparticles. Synthesis
S
of AgNps was confirmed by UV-Visible spectroscopy, surface plasmon resonance (SPR) peak was
observed at 470nm which indicates the poly dispersion of particles. The pH and temperature of
the medium plays important role in the synthesis of control shaped and sized nanoparticles. The
colour intensity of the aqueous solution varied with pH. In this study, at pH 9, the colour of the
aqueous solution was dark brown, whereas in pH 11 the colour was light brown; the colour
difference in the aqueous solution occurred due to the higher production of silver nanoparticles
and as the temperature increased absorbance was decreased which is inversely propotional to
time. The antioxidant activity of the silver nanoparticles was carried out using DPPH assay and
reducing power assay.
Keywords: Silver nanoparticles; Clitorea ternatea; Temperature; Ph
Clitoria ternatea
51
THERMAL, ANTI-FUNGAL AND PRIMARY ELECTROCHEMICAL STUDIES OF PALLADIUM N

NANOPARTICLES
A
N. John Sushma1, K. Mallikarjuna2, D. Prathyusha1, G. Narasimha3, G. Swathi1, B.V. Subba Reddy4 and B.
Deva Prasad Raju5, * N
1
Department of Biotechnology, Sri Padmavati Women’s University, Tirupati-517502, India. 2Department of Physics, O
Sri Venkateswara University, Tirupati – 517 502, India. 3 Department of Virology, Sri Venkateswara University, M
Tirupati - 517 502, India. 4Indian Institute of Chemical Technology, Hyderabad – 500 007, India. A
5
Department of Future Studies, Sri Venkateswara University, Tirupati - 517 502, India.
Tel: +91-94402 81769, E-mail id: drdevaprasadraju@gmail.com T
E
Green nanotechnology is one of the potential breakthroughs of biology, medicine, R
chemistry, and physics, sensors and material science. In this report, an attempt has been made I
to demonstrate a single step method for palladium nanoparticles of the size less than 5 nm using A
anti-cancer potent Piper longum leaf extract. The synthesized PdNPs were confirmed and L
characterized by several analytical techniques such as UV-Vis spectra, XRD, TEM, SAED, FTIR, TG- S
DTA and primary electrochemical analysis. The morphology and size obtained PdNPs are
spherical in size and in the order of 3-5 nm and the crystalline facets (111), (200), (211), (311) and
(222) face centered cubic respectively. The presences of the bio-organic moieties, which are
responsible for the formation of PdNPs, were analyzed with the Fourier transform infrared
spectroscopy. The thermal behavior of synthesized PdNPs was studied with TG-DTA analysis. The
bioactivity demonstrated by synthesized PdNPs was lead to an excellent clinical use as anti-fungal
material. We have demonstrated the modified carbon paste electrode using palladium
nanoparticles by means of cyclic voltammetry in a solution of 1M KCl and 1mM [Fe(CN)6]3-/4-.
Keywords: Biogenic preparation, Palladium nanoparticles, TG-DTA analysis, Anti-Fungal
activities, Electro-chemical studies.
52
SYNTHESIS AND CHARACTERIZATION OF GREEN SILVER NANOPARTICLES MEDIATED BY AEGLE N

MARMELOS (L.) LEAF EXTRACT
A
Sukumar Dandapat*, Manoj Kumar and Manoranjan Prasad Sinha
Department of Zoology, Ranchi University, Ranchi, Jharkhand-834008 N
Corresponding author e-mail: scholar.sukumar27@gmail.com O
M
Synthesis of green silver nanoparticle mediated by medicinal plant extract is easier, A
cheaper and ecofriendly. The bioactive phytochemicals like phenols, saponins, flavanoids, T
tannins alkaloids etc. of A. marmelos act as a reducing and capping agent during synthesis of E
green silver nanoparticles (AgNPs) for target specific action and delivery of drugs. Colour change R
from pale yellow to dark brown and highest absorption of spectrum at 200nm and a broad I
spectrum at 474 nm of UV-visible spectroscopy provides the first confirmation of synthesis of A
green AgNPs. FT-IR spectroscopy showed transmission peak at 3275 cm-1 corresponding to O-H
L
and H- stretch, 1604 cm-1 corresponding to C=C stretch, 1384 cm-1 corresponding to N=O bend,
S
1072 cm-1 corresponding to C=N stretch, 825 cm-1 corresponding to symmetric P-O-C stretching
and 750 cm-1 corresponding C-Cl and =C-H bending were provided the conformation about the
presence of alcohols and phenols, represents alkenes, as aliphatic nitro compound, represents
aliphatic amines, aliphatic phosphate, chloro alkane respectively. Final confirmation was
obtained by Scanning electron microscopy (SEM), which showed spherical and cubical in shapes
green AgNPs with diameter of 60nm to 120nm in and the average diameter of the particles were
of 70nm.
Keywords: Drug, nanoparticles, plants, disease.
53
SYNTHESIS OF METALOXIDE NANO COMPOSITES FOR SOLAR CELLS N

S.Shanthi#1 , Dr.M.Dharmendirakumar2
#1
Research scholar, Anna University, chennai. 2Assistant professor, Anna University, chennai.
A
Email: shanthiphd2011@hotmail.com, Mobile 9677268816 N
O
Synthesis of Titanium stannous chloride oxide nano composites was done by M
solvothermal method. This method is to provide a simple economic and effective technique to A
produced nanocomposites.The emission rate was analysed with the help of UV-Visible T
spectroscopy. The particle size was measured by scanning electron microscopy. The structure, E
morphology were investigated by XRD. The useful of the titanium stannous chloride nano R
composites was used to measure the efficiency of solar cell. I
Keyword-XRD, SEM, Nano composite A
L
S
54
COMPARATITVE ANALYSIS OF SECONDARY METABOLITES IN LEAVES OF CELERY (APIUM N

GRAVEOLENS) : A STUDY ON STABILITY OF CELERY REDUCED NANOPARTICLES.
A
R. Harish, M. Anbarasan, P. Rajasekar*
Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai-602 105, Tamil Nadu N
Email: harijin2730@gmail.com, anbarasananbum@gmail.com O
M
The development of bio-inspired metallic nanoparticles has been used in various fields A
including medicine. The celery (CE; Apium graveolens) displays multiple biological properties. T
Thus, the study aimed to biosynthesize organic and inorganic celery leaves extract reduced gold E
nanoparticles (OCE-AuNPs and ICE-AuNPs) and to evaluate time dependent stability. The R
aqueous OCE and ICE leaf extracts were prepared and biosynthesized celery based AuNPs. The I
NPs were confirmed preliminarily by their color transformation and the range of UV absorption. A
The secondary metabolites of celery leaves were preliminarily compared and quantified by HPLC
L
analysis. The initial color of CE extracts (brownish yellow) transformed to violet color after 12h
S
reaction with chloroauric acid. Both OCE-AuNPs and ICE-AuNPs showed a characteristic UV
absorption at 520nm. The stability of AuNPs was monitored from its UV absorption. Interestingly,
OCE-AuNPs displayed a gradual decrease in the extent of UV absorption from 12h when
compared with ICE-AuNPs demonstrating its instability. The HPLC analysis of CE extracts
displayed the presence of flavonoids (rutin, quercetin, kamferol, and luteolin), polyphenols (gallic
acid, ellagic acid, catechin), glycosides and terpenoids. However, the studied secondary
metabolites were significantly lower in OCE extract when compared to ICE. This suggests that its
time dependent instability is associated with lesser availability of secondary metabolites. Further
studies like advanced microscopic and spectral analysis of CE reduced AuNPs will light on the
exact reason(s) for its instability.
Keyword: Celery, gold nanoparticles, phytochemicals, HPLC analysis
55
INFLUENCE OF MOLAR CONCENTRATION ON OPTICAL AND ORPHOLOGICAL N

CHARACTERIZATION OF ETHYLENEDIAMINETETRAACETICACID (EDTA) ADDEDTIN SULPHIDE
A
(SnS) NANO FILMS BY SILAR METHOD
N
P Mani, K Manikandan, V. Helen, A.Janaki Ramya, & J Joseph Prince
Department of Physics, Anna University: BIT Campus, Tiruchirappalli 620 024 O
E-mail: maniprahaspathy@gmail.com M
A
Thin films of EDTA added SnS were prepared for solar cell application as absorber layer to T
obtain wider energy band gap. In this research work, thin films of Tin Sulphide (SnS) were E
deposited on glass substrate in room temperature by Successive Ionic Layer Adsorption and R
reaction method. In this technique, several coatings of Tin Nitrate (Sn (NO3)2) as cationic I
precursor and Sodium Sulphide (Na2S) as anionic precursor were made. Few drops of complexing A
agent ethylenediaminetetraaceticacid (EDTA) added to Tin nitrate. The changes in the properties
L
of SnS were studied, when a molar concentrations of the Tin nitrate and Sodium Sulphide is
S
changed. The deposition parameters such as Dip duration is 35 Seconds, Ex-dip duration is 5
Seconds, Number of cycles are 25 and Number of dips are 4 were optimized to obtain good
quality of SnS films. The optical and morphological properties of Ethylene Diamine Tetra Acetic
acid added Tin Sulphide thin films were characterized by UV-Vis spectrometry, FT-IR and SEM
techniques. The UV-Vis spectra of SnS films in the wavelength range of 200-900 nm have been
observed to find the direct band gap. The band gap energy (Eg = 2.42eV, 2.33eV, 2.27eV, 2.23eV
and 2.02 eV) decreases with increase in molar concentration (0.05M, 0.10M 0.15M and 0.20M.
Band gap decreases with increasing particle size.
56
QUALITATIVE SCREENING OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) B

PAMP I
P. Parameswari 1and R. Devika2
1
Research Scholar, Dept. of Biotechnology Sathyabama University, Chennai-600119.
O
2
Dept. of Biotechnology, Aarupadai Veedu Institute of Technology, Paiyanoor- 603104. T
Email id: eshwari_2007@yahoo.com E
C
In the world about 6000 plants are presently used as traditional folklore and in India over H
1500 plants species were identified to have high therapeutic values. Artemisia nilagirica (Clarke) N
Pamp which is an aromatic, herbaceous, perennial plant has been identified to have medicinal O
value. In the present investigation, the various parts (Leaves, stem, flower and root) of Artemisia
L
nilagirica (Clarke) Pamp plant was segregated, dried, powdered and stored for further
O
investigation. In the present study, a known amount of powdered root and stem sample were
G
subjected for qualitative screening of bioactive constituents by standard methods, using four
Y
different solvents (Hexane, Ethyl acetate, Methanol and Water). Around 16 phytochemical
analyses were carried out to identify their presence in root and stem samples. On comparative
study, ethyl acetate recorded the highest number of phytochemical constituents in both root and
stem samples. From the above results, root showed positive results for tannins, flavonoid and
phenols and stem showed positive results for tannins, flavonoids, saponins, terpenoids and
phenols.
Keywords: Phytochemicals, Bioactive, Phenols, Flavonoids, Qualitative.
Results of phytochemical analysis of stem of Artemisia nilagirica (Clarke) Pamp with water
solvent
57
HLA G 14bp INDEL POLYMORPHISM IMPLICATED IN GENETIC PREDISPOSITION TO ASTHMA IN

B
KODAIKANAL I
Renuka S1, Thenmozhi M2 and V.J. Kavitha3 O
1 & 2 - Research Scholar, 3 – Assistant Professor
Department of Biotechnology T
Mother Teresa Women’s University, Kodaikanal – 624 101. E
C
Asthma is a common and complex condition, with considerable heterogeneity both in its H
phenotype and in the underlying pathophysiology. In India, an estimated that 57,000 deaths were N
attributed to Asthma in 2004 (WHO 2004) and it was seen as one of the leading cause of O
morbidity and mortality in rural India. The HLA complex is a 3.6 Mb high-density gene region L
located at the 6p21.3 chromosome region, encompassing more than 200 genes. The HLA G gene O
is a nonclassical class I HLA locus is composed of eight exons and seven introns. In the 3’UTR HLA-
G
G polymorphism is the 14bp indel polymorphism that can influence HLA G expression through
Y
mRNA stability. Recent studies in asthmatic families and in a birth cohort at high risk for
developing asthma suggest a role for HLA G in asthma susceptibility. This study attempted to
understand asthma (type I hypersensitivity) in the context of HLA G in a hilly terrain because they
were more number of people affected by asthma in the hills when compared to the plains. We
studied 25 volunteers for HLA G 14 bp indel polymorphism through PCR SSP. We conclude that
‘ID’ genotype in HLA G 14 bp 3’ UTR polymorphism predisposes to asthma. We suggest that there
is heterozygote advantage through balancing selection in asthmatic patients to other unresolved
serious clinical conditions. Further studies are required as to understand why the heterozygotes
are in high frequency in asthma patients in Kodaikanal.
Keywords: HLA G, 14 bp indel, heterozygote advantage, balancing selection, polymorphism,
asthma.
58
EFFECTIVE TREATMENT OF DYEING EFFLUENT USING CRAB SHELL - CHITOSAN AS A NATURAL

B
COAGULANT
Ahila. K. G, Thamaraiselvi. C I
Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal, Tamil Nadu O
T
Textile industries are utilizing huge volume of water for dyeing and finishing process and E
discharge larger volume of colored wastewater to the aquatic environmentand water polluter. C
In the present study, physicochemical characteristics of raw dyeing effluent was analyzed and H
compared with EPA standard values. The analyzed sample was treated with Tt A (crab shell N
2g/100ml), Tt B (calcium oxide 2g/100ml) and Tt C (Chitosan-2g/100ml & CAC-1g/100ml). O
Detention time for the treatment was 1hr. The analyzed raw effluent showed the presence of L
higher pH (10), TDS (12,600mg/l), COD (784mg/l), chloride (4333mg/l), sulphate (912mg/l), and O
phosphate (14.5mg/l). All the values were beyond the permissible limit of EPA standard. G
Treatment A showed poor reduction of impurities such as color (16.2%), TS (36%), TDS (43%),
Y
alkalinity (40%), acidity (50%), chloride (76%), sulphate (32%), phosphate (17%) and COD (31%).
And treatment B treated sample resulted in good reduction of parameters includes TS (51%), TDS
(46%), acidity (50%), choride (77%), sulphate (45%), phosphate (38%) and COD (50%). But
increase the pH of dye effluent from 10 – 12. The dye effluent was subjected to Treatment C
resulted in higher reduction of pollutants. The percentage reduction of color, odor, TS, TDS,
alkalinity, acidity, chloride, sulphate, phosphate and COD were 76.2%,100%, 62%, 60%,59%,
75%, 85%,52%,42% and 66% respectively. And the pH was gradually reduced to neutral. The CAC
treated effluent showed maximum removal of color (100%), odor (100%), TS (88%), TDS (89%),
alkalinity (74%), acidity (75%), chloride (89%), sulphate (64%), phosphate (78%) and COD (85%).
After, Treatment with chitosan and CAC the physico-chemical values were below the standard
limit.Ehe three different treatment process, Treatment C is an efficient technique for the
treatment of dye effluent. Hence, it was concluded that natural polyelectrolyte chitosan can be
used as an alternative for chemical coagulant and the biological sludge generated during
treatment can be converted into compost and can be used for fertilizer in agriculture field also.
Keywords: Dyeing effluent, EPA-Environmental protection agency coagulant.
S.NO Parameters Values (mg/L)

1 Color Dark green
2 Odour Unpleasant
3 pH 10
4 Total solids 15,700
5 TDS 12,600
6 TSS 3,100
7 Alkalinity 1600
8 Acidity 100
9 Chloride 4333
10 Sulphate 912
11 Phosphate 14.5
12 COD 784
Physico-chemical characteristics of dyeing effluent
59
ISOLATION AND SCREENING OF BIOSURFACTANT PRODUCING MARINE ACTINOMYCETES

B
Mobeen Shaik1, G.Girijasankar2, T.Prabhakar3, M.Iswarya4
1,2,3,4
A.U College of Pharmaceutical Sciences, Pharmaceutical Biotechnology Division, Andhra University, I
Visakhapatnam-530003, Andhra Pradesh. O
Email id: shaik.mobeen@gmail.com, Mob: 7893154858 T
E
Biosurfactants are surface-active substances synthesised by living cells. Interest in C
microbial surfactants has been steadily increasing in recent years due to their diversity,
H
environmentally friendly nature, possibility of large-scale production, selectivity, performance
N
under extreme conditions. In the present study, 15 marine actinomycetes from Visakhapatnam
O
harbour oil contaminated marine sediment sample were isolated , screened in Humicacid-salts-
L
vitamin agar medium, starch casein agar medium (SCA), Zobell marine agar medium (ZMA,
Himedia) and evaluated for biosurfactant production by hemolytic assay, lipase production test, O
Phenol-H2SO4 test, drop collapse test, emulsification index. Out of 15 isolates, VHOCMS-1 isolate G
showed promising positive results for all the above mentioned tests. This isolate was also Y
screened for dye decolourization activity (CTAB method) a semi quantitative assay for the
detection of anionic extracellular glycolipid (rhamnolipid). All the studies revealed that the isolate
produced rhamnolipid biosurfactant.
Screening of Marine Actinomycetes, b) CTAB method, c) Phenol-H2SO4 test
Production of Biosurfactant in Kim’s Medium b)Antimicrobial activity of supernatant
60
OPTIMIZATION OF MEDIA FOR THE PRODUCTION OF KERATINASE ENZYME

1 B
T.P.Rajesh*, 1B.Anandaraj 2N.Ilavarasan,2P.Saranya Devi
1
Department of Biotechnology, Anna University Trichy 2Department of Civil Engineering, Anna University Trichy I
Email id: 1tprajesh2009@gmail.com, biotechraj@gmail.com O
T
Keratin are insoluble fibrous proteins found in hair, wool, feather, nail, horns and other E
epithelial covering which is rich in beta helical coil linked through cysteine bridges. Keratinase C
belongs to the class hydrolase which are able to hydrolyse insoluble keratins more efficient than H
other proteases. The keratinous waste can be biologically degraded by enzymes or microbes itself N
to form useful products. The ability of strain Proteus mirabilis to utilize azokeratin as a substrate O
was tested. Among various carbon sources the maximum keratinase activity was found in glucose
L
and among various nitrogen sources the maximum keratinase activity can be found in casein.
O
Similarly optimum temperature and pH for the enzyme activity was found. Medium optimization
G
was carried out by using Plackett-Burmann method. Specific enzymatic assays demonstrate that
Y
keratinase can act on a large variety of soluble and insoluble protein substrates. Extracellular
keratinase may be a useful alternative and eco-friendly route for handling the abundant amount
of waste feathers or for applications in other industrial processes. Keratinase also hydrolysed the
outer epithelial sheath of hair roots provoking depilation. These data suggest the potential of this
enzyme for application in ecologically-friendly leather processing.
Keywords: Keratinase, Proteus mirabilis, optimization, Plackett-Burmann method, enzyme.
Screening on Milk agar plates
61
BIOREMEDIATION OF SUGAR WASH USING NATURAL SCAVENGERS

B
Nithya.A, B. SowmiyaRajalakshmi, K.G. Ahila, A. AncyJenifer, Thamaraiselvi. C
Dept. of Biotechnology, Mother Teresa Women’s University, Kodaikanal-624101. I
Email id: ckthamarai@yahoo.com O
T
Pollution control board classify distillery industry is one of the most polluting industry in E
India. Distillery is an ancillary industry of sugar industry. Effluent emanating from distillery C
industries is dark colored, acidic, with high biological oxygen demand, chemical oxygen demand, H
and liquid consisting of biodegradable organic and inorganic constituents, which cannot be N
disposed directly into water bodies. Hence, the effluent released from a distillery industry was O
collected and were analyzed for various physico-chemical characters. Due to the higher
L
concentration of the spent wash, it needs dilution. Hence, the problem of water scarcity, it was
O
diluted with the sugar effluent at 1:1 ratio (spent wash 50 ml + sugar effluent 50 ml). Thus, the
G
obtained sugar wash was biologically treated using the indigenous microorganisms and consortia.
Y
The isolated microorganisms were identified as Enterobacter (T1) sp., Corynebacterium (T2) sp.,
Microccocus (T3)sp.,Corynebacterium(T4)sp and Pseudomonas sp. The treatment efficiency of
microorganisms was found to increase with time. All the organisms showed poor reduction of
colour, TDS and COD after the time period of 48hrs and after 72 hrs. There was no considerable
reduction of the above-mentioned parameters at 24 hrs. Among the micro-organism,
Corynebacterium (T2)sp., has shown higher reduction of TDS (12.76%) , COD(16%) and
color(9%) removal after the time period of 96 hours. However, the consortia showed higher
reduction of 28%, 32%, 35% of colour, TDS and COD respectively after the 96 hours treatment.
The results revealed that the possibility of using indigenous micro – organisms especially
consortia for the treatment of sugar wash.
Keywords: Physico-chemical, indigenous, sugarwash, spentwash, effluent, Total Dissolved Solids
Effect of microbes on the colour, TDS and COD removal from the sugar wash (96 hrs)
Sl No Microorganism Colour Removal % TDS Reduction % COD Reduction %
1 Enterobacter(T1) sp. 6 9 13
2 Corynebacterium(T2) sp. 9 12.76 14
3 Microccocus(T3) sp. 6 9.2 13
4 Corynebacterium(T4) sp. 8 12 16
5 Consortia 28 32 35
62
PACKED BED COLUMN ADSORPTION OF CR (VI) ONTO ACID TREATED CODIUM TOMENTOSUM
B
BIOMASS
P. Suresh Babu1, S. Eswaramoorthi2, T.P. Rajesh3 and B. Anandaraj4 I
1
Department of Biotechnology, Sree Sastha Institute of Engineering and Technology,Chembarambakkam, O
Chennai–600123, TN 2Department of Civil Engineering, BIT Campus, Anna University,Tiruchirappalli–620024, TN T
3, 4
Department of Biotechnology, BIT Campus, Anna University, Tiruchirappalli–620024, TN E
Email id: dranandaraj74@gmail.com, Mob: 9790915036
C
H
Packed bed column study was conducted by using acid activated Codium tomentosum
biomass for the adsorption of chromium (VI) from aqueous solution. SEM analysis was carried N
out to study the surface topology of the biomass and SEM micrographs are subjected to fractal O
analysis to examine the surface roughness and porosity of the biomass. The influence of bed L
depth and flow rate was investigated. The breakthrough curves obtained for the experimental O
data elucidate the importance of bed depth and flow rate. To evaluate the adsorption column G
performance, Bohart-Adam’s, BDST, Thomas and Yoon–Nelson model was used. BDST Model Y
expressed the good linearity for the experimental data. Linear regression coefficients (R2 values)
of Thomas and Yoon–Nelson model showed a good linearity when compared with Bohart-Adam
model. The potential of the acid treated Codium tomentosum biomass in the removal of
chromium (VI) was found to be good. Hence, it was concluded that acid treated Codium
tomentosum biomass can be used as an effective adsorbent for the removal of chromium (VI)
ions.
Keywords: Codium tomentosum, Cr (VI) removal, Column studies, Fractal analysis, Acid
activation.
Bed height Flow rate Uptake capacity tb (h) te (h) ∆t (h) Cr(VI) Removal
(cm) (mg/ml) (mg/g) percentage
4 2 24.40 7 33 26 80.54
6 2 21.64 9 40 31 83.47
8 2 17.67 9 41 32 80.70
10 2 15.44 14 46 32 83.54
10 4 14.20 8 23 15 81.43
Column data and parameters obtained at different flow rates and bed heights
63
COLORIMETRIC DETECTION OF S.AUREUS USING PEPTIDE NUCLEIC ACID AND COLLOIDAL

B
GOLD NANOPARTICLES I
M.Ananda Chitra1, N.Daniel Joy Chandran2, A.Ramesh1 and P.Ponnusamy1 O
T
1
Department of Veterinary Microbiology, Veterinary College & Research Institute, Orathanadu – 614 625,
Thanjavur District ,2Department of Veterinary Microbiology, Madras Veterinary College, Chennai-600 007
E
Email: m.anandachitra@tanuvas.org.in Mob: 94421 74491
C
H
Peptide nucleic acids (PNA) are DNA analogues in which the entire sugar-phosphate
N
backbone is replaced by a charge neutral polyamide backbone. Free PNA causes aggregation of
O
colloidal gold nanoparticles and it was inhibited by the presence of complementary target DNA.
Hence, the present study was aimed to visually detect S.aureus which causes a wide variety of L
pyogenic infections in man and animals specifically and rapidly. Citrate capped gold nanoparticles O
were synthesized using standard protocol and custom synthesized PNA probe specific for G
S.aureus was used. Although 75 pmole PNA concentration of was able to agglomerate 100 µl of Y
colloidal gold nanoparticles, 100 pmole of PNA induced clear colour change from brick red to blue
colour. Complementary target DNA of 1-200 pmoles were hybridized with 100 pmole of PNA for
10 min and then colloidal gold nanoparticles was added to make up 100 µl of test solution. It was
found that 50 pmole target DNA was sufficient to form charged PNA-DNACOMP mixture and retain
the stability of colloidal gold nanoparticles to prevent colour change as shown in Fig 1. The test
was performed in the presence of non-complementary DNA with or without complementary DNA
and was found that at 100 & 200 pmole of non-complementary DNA caused bluish pink colour of
colloidal nanoparticles, whereas higher concentration of non-complementary DNA induced
aggregation of colloidal gold nanoparticles. The experiment was carried out to detect S.aureus
isolated from clinical specimen and it could detect all fifteen isolates of S.aureus specifically.
Hence, this principle can be used to develop a rapid disease diagnostic test kit that can be used
in basic clinical laboratories.
Complementary target DNA concentration (in pmoles) with 100

pmole of PNA probe
1 10 25 50 75 100 150 200 -

ve +ve
Photographs and corresponding adsorption spectra of PNA-

containing gold nanoparticles solutions in the presence of
increasing amount of complementary DNA
64
BIOLOGICAL CONTROL OF DAMPING OFF AND STEM ROT OF TOMATO (Lycopersicon

B
esculentum Mill.) USING AN ANTAGONISTIC ACTINOMYCETE, SACCHAROPOLYSPORA ERYTHRAEA.
Prabha S. B. and K.Murugesan I
CAS in Botany, University of Madras, Guindy campus, Chennai- 25, Tamilnadu, India. O
E-Mail id: pkprabha27@gmail.com Mob: 9445319254 T
E
Actinomycetes have been found to protect plants against their diseases. Stem rot and C
damping off of tomato caused by Sclerotinia sclerotiorum and Rhizoctonia solani, respectively are H
one of the major yield- limiting factor for tomato production. The present study aimed at the N
isolation of actinomycetes from different rhizosphere soil of tomato plants and to check their O
antagonistic effect against two dreadful phytopathogenic fungi such as Rhizoctonia solani and L
O
Sclerotinia sclerotiorum.
G
The selected isolate was further identified and characterized using physiochemical and
Y
biochemical methods. Fifty strains were isolated and the strain AMP14 showed significant activity
against the two fungi, R.solani and S.sclerotiorum on preliminary screening by dual culture plate
method as well as crude was also checked for its antagonistic effect. Further for the species level
identification of the actinomycete, AMP14, was identified using 16srDNA sequencing was used
and found to be Saccharopolyspora erythraea. The presence of the compounds was analyzed
using thin layer chromatography (TLC). Based on the TLC analysis, further column was carried out
using sephadex LH-20. The purified compound was further confirmed by TLC and GC-MS, UV,
NMR, FTIR characterization.
Keywords: Biological control, Saccharopolyspora erythraea, Phytopathogens
Colony morphology of AMP14 Reverse side pigmentation
65
INVITRO AND INVIVO BIOACTIVITY SCREENING OF ALPHA ASARONE

B
Ragavee, J.Arul Daniel, S.Asha Devi
School of Bio Sciences and Technology VIT University Vellore- 14, TamilNadu, India I
Email id: ashaselvaraj74@gmail.com O
T
Alpha asarone is a phytochemical compound in the rhizome oil of A. calamus. It was E
found to be a potential antithrombotic, antimicrobial, insecticidal and nematicidal and C
antifeedant agent. Alpha asarone is also a good natural radio protective agent with many H
therapeutic implications (Divyasree and Nair, 2011). Antioxidant phytochemicals of plants exert N
their effect by neutralizing highly reactive radicals. Among thousands of phytochemicals found in O
our diets or traditional medicines, polyphenols and carotenoids stand out as the two most
L
important groups of natural antioxidants. However, collectively these phytochemicals are good
O
antioxidants; the roles and effect of individual compounds are often not well known. Thousands
G
of polyphenols have been identified from various plants and their antioxidant properties were
Y
proved by many researchers. In the present study in vitro antioxidant activity of alpha asarone
was evaluated using scavenging assays of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl
radicals, superoxide anion radicals, chelating ability of ferrous ion. The in vivo study was carried
out to evaluate the analgesic, antipyretic and ulcerogenic activities of alpha asarone on Swiss
albino mice. Alpha asarone exhibited strongest antioxidant activities in scavenging DPPH radicals.
It also exhibited best performance in chelating metal ion. The in vivo activity of the alpha asarone
was compared with the standard drug paracetamol. The result illustrated very strong analgesic,
antipyretic effect, with the absence of gastric damage by the alpha asarone.
66
BIOTRANSFORMATION OF CLOZAPINE TO NORCLOZAPINE AN ACTIVE METABOLITE BY

B
CUNNINGHAMELLA ELEGANS
S. Varalaxmi1, M. Vidyavathi2 I
Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Viswa Vidyalayam, Tirupathi – 517 590, A.P., India. O
Email id: vara6veda12@gmail.com, Mobile: 09849199376 T
E
The biotransformation of clozapine a CYP1A2 substrate was investigated using eight C
different fungal cultures by fermentation technique. Among all the selected fungi H
Cunninghamella elegans has shown the ability to biotransform the clozapine. The metabolite N
found from culture extracts of Cunninghamella elegans was identified by HPLC analysis and O
further the metabolite was isolated and structure of metabolite was elucidated using Nuclear
L
Magnetic Resonance (NMR) and mass spectroscopy. The metabolite structure was confirmed as
O
norclozapine by above methods. Hence Clozapine was transformed into its active metabolite
G
norclozapine by Cunninghamella elegans. The microbial transformation of clozapine was similar
Y
to metabolism of clozapine in mammals. Thus the Cunninghamella elegans can be used as
complementary tool as an in vitro models of drug metabolism studies in mammals and can also
be used to produce active metabolite for further pharmacological and toxicological studies.
Name of the Organism Retention time (min.)

( Blank I) (Blank II) (Control) Sample
Drug control Culture control Pure
Clozapine
Aspergillus flavus [MTCC 2.0 2.0 - 2.0
1783] - 4.0 - 4.0
8 - 8 8
Aspergillus ochraceus 2.2 2.2 - 2.2
[NCIM 1140] - 4.2 - 4.2
8.2 - 8.2 8.2
Aspergillus terreus [NCIM 2.1 2.1 - 2.1
657] 4.1 4.1 - 4.1
8.1 - 8.1 8.1
Cunninghamella 2.2 2.2 - 2.2
blakesleeana [MTCC - 4.2 - 4.2
3729] 8.2 - 8.2 8.2
Cunninghamella elegans 2.1 2.1 - 2.1
[NCIM 691] - 4.1 - 4.1
8.1 - 8.1 8.1
Cunninghamella elegans 2.2 2.2 - 2.2
[NCIM 689] - 4.2 - 4.2
8.2 - 8.2 8.2
- - - 3.5*
HPLC data of Clozapine and its metabolite from fungal culture extracts
67
SYNTHESIS AND MICROBIAL ACTIVITIES OF SOME CHALCONES

B
V. Usha1, V.Thangaraj2
1. Assistant Professor, Department of Chemistry, University College of Engineering, Panruti I
2. Assistant Professor, Department of Chemistry, Anna University BIT Campus O
Email id: vushachem2004@gmail.com, thangamraj@gmail.com T
E
Chalcones are unsaturated ketone containing the reactive ketoethylenic group. These are C
coloured compounds because of the presence of chromophore –CO-CH=CH-. Series of aryl
H
Chalcones have been synthesised by Crossed Aldol condensation using Ultrasonics. This is
N
pollution free technique, gives more yield. Chalcones possess various bio and multi prolonged
O
activities. These are examined against respective microbes – bacteria’s and fungi.
L
Keywords: Chalcones, Crossed Aldol condensation, Invitro- petridish Bauer- Kirby method.
O
G
Y
Antibacterial activity of compounds

10
9
8
ZONE INHIBITION (cm)
7
B.subtilis
6
M.luteus
5
S.aureus
4
E.coli
3
P.aeruginosa
2
k.pneumoniae
1
0
68
A SPECIAL EMPHASIS ON MITOCHONDRIAL DNA IN BREAST CANCER THERAPY

B
R. Jayalakshmi, T. Keerthana, P. Selvamani and S. Latha
I
Department of Pharmaceutical Technology, Anna University, BIT Campus, Tiruchirappalli – 620 024, Tamil Nadu,
Email id: rjayalakshmi.au@gmail.com O
T
Breast cancer is the most common cancer in women, and this prevalence has a major E
impact on health worldwide. It is the second common diagnosed cause of cancer death in the C
developing and developed countries and accounts for 23% of the total cancers. Different studies H
reported that breast cancer accounts for 14% of all cancer deaths in females. It is estimated that, N
it occurs in one out of eight women during her lifetime. Breast cancer can occur by different O
L
factors like genetics and various environment factors. Mitochondrion is the power house of the
O
cell play important roles in cellular energy metabolism, free radical generation, and apoptosis.
G
Mitochondrial defects have long been suspected to play an important role in the development Y
and progression of cancer breast cancer. In particularly the mitochondrial DNA (mtDNA) is
becoming the study hotspot for its alteration in correlation with its tumorigenesis in cancer/
breast cancer. Because mitochondrial DNA have high susceptibility to mutations and limited
repair mechanisms when compared to nuclear DNA. Mutations in the mitochondrial genome
leading to mitochondrial dysfunction and cancer occurrence. Thus the mtDNA targeted
bioenergetic metabolism will be a promising chemotherapeutic strategy for breast cancer
therapy.
Keywords: Breast cancer, Mitochondria, Mitochondrial DNA, Chemotherapy and Bioenergetic
metabolism.
69
ENHANCEMENT OFPOST-HARVEST AND SHELF LIFE OF CARICA PAPAYA USING ALOE

B
VERA GEL BASED COATINGS.
Nandakumar.A1, Ebenezer Samuel King. J, Arulvel.R I
*M.tech-Biotechnology, K.S.Rangasamy College of Technology, Tiruchengode-637215, and Namakkal dist. O
**Assistant Professor, Dept. of Biotechnology, K.S.Rangasamy College of Technology, Tiruchengode- T
1
637215, and Namakkal dist. E
E-mail id: nandhua91@gmail.com Mobile No.: 7845659621
C
H
Papaya (Carica papaya) is one of the main tropical fruits of India. The desiccation of fruits
and perishable nature of papaya is a major drawback during transportation to distant markets N
and storage during glut in the market. Aloe vera gel, mainly composed of polysaccharides, has O
been recently explored as an edible coating owing to its antifungal activity. To improve the L
performance of edible coatings, various substances/chemical additives have been incorporated. O
The present study was carried out to evaluate the ability of Aloe vera gel based antimicrobial G
coatings to reduce/control the loss of post-harvest fruit quality in papaya. Freshly harvested Y
papaya fruits were coated with AGI (50% Aloe vera gel containing citric acid) and AGII (50% Aloe
vera gel containing tartaric acid). The coated and uncoated (control) fruits were stored at 30±3ºC
for 18 days. Physical (PLW, fruit size), chemical (pH, titrable acidity) and sensory characteristics
(color, taste and firmness), fruit disease index (FDI) and marketability were analyzed at regular
intervals during storage period. The AGI coated fruits survived the storage period of 15 days and
the AGII coated fruits survived the storage period of 18 days, whereas, all the uncoated controls
decayed within 10 days. The uncoated/control fruits exhibited significantly greater changes in all
the parameters tested. AGII coated fruits exhibited least changes than AGI coated fruits
Keywords: - Carica papaya, Aloe vera gel, citric acid, tartaric acid, PLW (Physiological Loss of
Weight).
STORAGE DAYS Vs FDI

120
100
80
FDI
60 Control
40 AGI
20 AGII
0
5 10
STORAGE DAYS
70
DEVELOPMENT OF SMART DEVICE FOR DETECTION OF MICROBIAL BIOFILM FORMATION

B
Ajitha Bharathi Annadurai1, Akshaya Kumari Selvan1, Deepika Dhandapani1, Pravin Kumar Subbaraj1,
I
Guruprakash Subbiahdoss1
1
Department of Biomedical Engineering, SSN College of Engineering, Old Mahabalipuram, Road, Kalavakkam - O
603110, Tamilnadu T
Email:ajithabharathi@gmail.com, Mob: +91 967732642 E
C
Bacterial biofilm formation is a major problem in dentistry, artificial urinary tracts, H
industries and drinking water plants. Biofilm detection is difficult with conventional technologies.
N
It is known that bacterial proliferation changes the pH of the surrounding environment due to
O
the substances they release. Therefore, precise measurement of pH changes induced by
L
proliferation and metabolism would contribute to the detection of biofilm formation. Here, we
O
used an Ion Sensitive Field Effect Transistor (ISFET) to detect the change in pH of the microbial
environment. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were G
cultured and pH changes in the suspension were measured using an ISFET sensor. The schematic Y
representation of the study was shown in Figure 1. The measurements were displayed real time
in a LCD monitor and the data is stored in the computer as a text file. Once there was an onset of
infection or contamination (pH = 4.5), the device triggers an alarm. In addition, the device was
designed to communicate to a remote mobile or console.
Schematic representation of the study: Ion Sensitive Field Effect Transistor for detection of microbial biofilm
formation
71
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC P

EXTRACT OF LEAVES OF ACTINODAPHNE BOURDILLONII GAMBLE H
S.Deepa and S. Selvakumar
Department of Natural Resources and Waste Recycling, School of Energy, Environment and Natural Resources, A
Madurai Kamaraj University, Madurai – 625021. R
Email: kumarmkuenergy@gmail.com M
A
The aim of this study is to screen the phytochemicals present in the methanolic extract C
of leaves of Actinodaphne bourdillonii Gamble and its antifungal activity. The in vitro antifungal E
evaluation was carried out by disc-diffusion method against four fungal strains Aspergillus Niger, U
Aspergillus flavus, Candida albicans and Candida tropicalis. Preliminary phytochemical studies
T
revealed the presence of alkaloids, cardiac glycosides, saponin, tannin, terpenoids, and
I
phlobatannins in the methanolic extract of leaves of A. bourdillonii Gamble. Antifungal activities
C
of extract were tested with four different concentrations (50, 100,200 400 mg/ml). Largest
antifungal activity Aspergillus Niger while least antifungal activity Candida tropicalis. A
L
Keywords: phytochemical, methanolic, Actinodaphne bourdillonii Gamble, antifungal, extract T
E
S.NO Parameters Result C
1 Phlobatannins Present H
N
2 Terpenoids Present
O
3 Phytosterols Absent
L
4 Flavonoid Absent
O
5 Tannin Present G
6 Saponin Present Y
7 Cardiac glycosides Present
8 Alkaloids Present
Phytochemical constituents present Methanolic leaves extract of Actinodaphne bourdillonii
72
ANTIMICROBIAL ACTIVITY STUDY OF FLAVONOIDS AND SALICYLIC ACID EXTRACTED FROM P

TAGETES ERECTA LINN. H
R. Devika1 and Justin Koilpillail2
1
Research Scholar, Dept. of Biotechnology, Sathyabama University, Chennai A
2
Dept. of Botany, St. Joseph’s College, Trichirappalli R
Email: vineethdevika@gmail.com M
A
Plants are a great source of bioactive compounds which are rich sources of therapeutic C
agents. Out of 2, 50, 000 – 5, 00,000 plant species on Earth, roughly 10% have been studied E
chemically and pharmacologically. Tagetes erecta Linn. An ornamental plant used traditionally in U
all occasions has been selected to identify for its medicinal value efficiency. A constant screening
T
of the phytochemical constituents has been carried out and Tagetes erecta Linn. Flower sample
I
proved to have high therapeutic agents than stem, leaves and root parts. The floral extracts by
C
column chromatography was obtained and the phytochemical constituents were separated by
TLC and then the individual constituents were screened, eluted and purified by standard methods A
and were stored in air tight containers for future investigations. In the present investigation, the L
purified flovonoid and salicylic extracts were subjected to antimicrobial activity with T
Acinetobacter Baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, E
Klebsiella pneumonia and Candida albicans by Kirby Bauer disk diffusion method. Staphylococcus C
aureus recorded the maximum zone of inhibiton flollowed by Klebsiella pneumonia, Candida H
albicans, Pseudomonas aeroginosa and Bacillus subtilis in flower. Acinetobacter baumannii N
showed resistant against flavonoids extract of Tagetes erecta Linn. The salicylic acid extract also O
registered the maximum zone of inhibition with Staphylococcus aureus and other organisms did L
not show any significant zone of inhibition. O
G
Y
Zone of Inhibition of S.aureus Zone of Inhibition of A.baumannii
73
NEW PARAMETERS IN ASSESSING CARDIOVASCULAR RISK IN BOTH NON DIABETIC AND P

DIABETIC HYPERLIPIDEMIA SUBJECTS: A CASE CONTROL STUDY H
Pusapati Madan Ranjit 1, 2*, G. Girijasankar 3, P. Ramesh Babu4
1
NRI College of Pharmacy, Pothavarappadu (V), Agiripalli Mandal, Krishna District, A.P, India. A
2
Research scholar, Department of pharmacy, JNTUK, Kakinada, A.P, India R
3
University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, A. P, India. M
4
Chief Cardiologist, Dr.Ramesh Cardiac & Multispeciality Hospital LTD, Vijayawada, A.P, India
A
Email id: madanranjit@gmail.com, Mobile: 08196345367
C
This study aimed to assess cardiovascular risk in both non diabetic with hyperlipidemia E
(NDH) and diabetic with hyperlipidemia (DH) subjects compared with healthy control (C) by using U
new atherogenic indices. This study was carried out at Dr. Ramesh Cardiac & Multispeciality T
Hospital LTD, Vijayawada, A.P, India, about 266 subjects were participated. This study was I
approved by institutional ethics committee. Among these subjects divided into 133 non diabetic C
hyperlipidemic subjects, 67 diabetic hyperlipidemic and 66 healthy subjects. Fasting blood A
samples were analysed for FBS( Fasting Blood sugar), total cholesterol (TC), triglycerides (TTG), L
low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), Non – T
HDL cholesterol. When FBS and Lipid profiles of both non diabetic and diabetic hyperlipidemia E
compared were compared with healthy subjects. Fig 1: Showed comparison the mean ± SEM lipid C
profiles (mg/dl) of controls(C) and both non diabetic (NDH) and diabetic hyperlipidemia (DH) H
subjects. When compared between NDH and DH, atherogenic index of plasma (AIP) value N
significantly higher (p<0.05*). Our conclusion is these ratios can contribute significantly to the O
estimation/prediction of risk of CAD especially when absolute values of lipid profile parameters
L
are not markedly deranged or in centers with insufficient resources and facilities.
O
Keywords: Diabetic, Hyperlipidemia, Atherogenic indices, Coronary Artery Disease.
G
Y
300
250
200
mg/dL
150
C
100
NDH
50
DH
0
Lipid profile Parameters
Showed comparison the mean ± SEM lipid profiles (mg/dl) of controls(C) and both non
diabetic with hyperlipidemia (NDH) and diabetic with hyperlipidemia (DH) subjects
74
EFFECT OF NICORANDIL ON THE PHARMACODYNAMICS OF METFORMIN IN ANIMAL MODELS P

Suresh Goli1* and K. Eswar Kumar2
1*
Research Scholar, Jawaharlal Nehru Technological University, Kakinada- 533003, Andhra Pradesh
H
2
Pharmacology Division, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam – 530 A
003, Andhra Pradesh R
Email id: sureshgoli@hotmail.com, Mobile: 09966374700 M
A
The study was conducted to find the influence of Nicorandil (K+ channel Opener, which is
C
widely used for hypertension) on the hypoglycemic activity of Metformin (Biguanide derivative),
E
which is widely used for the type-II diabetes management in humans. Single Dose Interaction
U
(SDI) followed by Multiple Dose Interaction (MDI) study was conducted in Rats (Normal &
Diabetic) and Normal Rabbits consisting of 6 animals in each group, with oral administration of T
selected doses of Metformin, Nicorandil and their combination (SDI & MDI) with adequate I
washout periods in between treatments. Blood samples were collected from rats by Retro orbital C
plexus and in rabbits by marginal ear vein puncture at regular intervals of time. All the blood A
samples were analysed for glucose by GOD/POD method. Metformin produced hypoglycemia / L
antihyperglycemia in rats (Normal & Diabetic) and rabbits at 3h, whereas Nicorandil produced T
peak hyperglycemia at 4h in rats (Normal & Diabetic) and 6h in rabbits. Nicorandil when given in E
combination with Metformin in SDI followed by MDI study showed significant reduction of C
Metformin peak action level in both Normal (SDI: *p≤0.05 ; MDI: **p≤0.01), Diabetic rats (SDI: H
***p≤0.001 ; MDI: ***p≤0.001) and Normal rabbits (SDI: *p≤0.05 ; MDI: ***p≤0.001). Similar to N
the theoretical expectation, the pharmacodynamic effect of Metformin was significantly changed O
when combined with Nicorandil in rats (Normal & Diabetic) and rabbits indicating that peer L
research need to carry out to confirm the mechanism established. O
G
Keywords: Metformin, Nicorandil, Single Dose Interaction (SDI), Multiple Dose Interaction (MDI),
Y
Hypoglycemia, Hyperglycemia.
TIME METFORMIN NICORANDIL SDI MDI

(HRs) Mean SD Mean SD Mean SD Mean SD
3 25.40 1.82 -9.64 4.79 20.35* 3.86 18.41*** 2.38
(Diabetic) (Normal) (Normal)
Rabbit
4 19.88 4.94 -11.22 6.32 12.12* 2.34 10.12** 2.27

6 17.11 4.28 -12.67 3.35 11.08** 3.23 9.81* 1.72
3 22.63 1.22 -20.62 6.45 22.63* 1.22 18.24** 2.32
4 12.86 2.51 -22.68 11.37 12.86* 2.51 10.34* 4.82
Rat
6 10.93 3.02 -16.76 15.98 10.93 3.02 9.91* 3.91

3 45.60 2.96 -19.63 2.93 30.61*** 2.55 27.28*** 1.01
4 30.03 5.26 -23.53 2.55 22.15* 3.15 17.79** 2.04
Rat
6 21.42 4.05 -21.77 3.65 19.50 1.91 16.35* 1.23
Summary of Mean percent of blood glucose reduction (mg/dL) in Rats (Normal & Diabetic) and Rabbits
75
DETERMINATION OF BIOACTIVE CONSTITUENTS OF LEAVES OF CORCHORUS AESTUANS (L.) BY P

GC - MS ANALYSIS H
Dhanalakshmi R*, Manavalan R
Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram - 608002 Tamilnadu, India A
Email id: dhanama@gmail.Com R
M
The present work represents the possible bioactive constituents present in the ethanol A
extract of leaves of Corchorus aestuans (L.) using GC-MS technique. The ethanol extract of leaves C
were subjected to GC- MS analysis using GC Clarus 500 (Perkin Elmer) with Elite- 5MS E
(5%Diphenyl / 95% Dimethyl poly siloxane, 30mm x 0.25mm x0.25μm df). The 2 μl extract sample U
injected and the components were separated using Helium (1mL/min) as the carrier gas. T
Bioactive constituents were detected by the Turbo mass gold detector (Perkin Elmer) with the I
aid of the Turbomass 5.2 software. GC–MS chromatogram of the ethanol extract of leaves of
C
Corchorus aestuans in fig: 1 clearly showed fourteen peaks indicating the presence of fourteen
A
phytochemical compounds. The identification of the phytochemical compounds was confirmed
L
based on the peak area, retention time and molecular formula. The analysis revealed the
T
presence of fourteen different bioactive constituents namely 3, 7,11,15-tetramethyl-2-
hexadecen-1-ol (5.6%), Trans-2-undecen-1-ol (1.26%), E-7-Tetradecenol (1.97%), n- E
Hexadecanoic acid (25.82%), Phytol (22.34%), 9,12,15-octadecatrienoic acid, methyl ester, C
(Z,Z,Z)- (20.23%), Docosanoic acid, ethyl ester (1.99%), 1-Eicosanol (2.11 %), 9,9- H
dimethoxybicyclo[3.3.1] nona-2,4-dione (0.60%), Heptadecanoic acid, heptadecyl ester (0.95%), N
Pentadecanoicacid,2,6,10,14-tetramethyl-,methylester(0.91%), 3-Hexadecycloxycarbonyl-5-(2- O
hydroxyethyl)-4-methylimidazolium ion (0.90%), Squalene (8.03%), Vitamin E (7.24%). The L
phytochemical compounds recognized through GC-MS analysis showed many biological O
activities. The presence of various bioactive compounds confirms the application of Corchorus G
aestuans in various disorders. Y
GC - MS Chromatogram of ethanol extracts of leaves of Corchorus aestuans
76
MOLECULAR DOCKING STUDIES OF CEFTRIAXONE SODIUM WITH APOPTOSIS PROTEIN IN P

COLORECTAL CANCER H
Manimekalai P, Manavalan R
Department of pharmacy, Annamalai University, Annamalai nagar Chidambaram 608002
A
Email id: mekalaivel@gmail.com R
M
Molecular docking is a key tool in structural molecular biology and computer-assisted A
drug design. The goal of ligand—protein docking is to predict the predominant binding C
mode(s) of a ligand with a protein of known three-dimensional structure. Docking study was E
performed by Schrodinger-Maestro 9.3.5 Version In the present study was performed to U
identify the binding energy, H bond interaction, hydrobhobicity and lipophobicity of ligand T
ceftriaxone sodium with Apoptosis protein (which is involved in colon cancer). While I
performing docking simulation, information on feasible conformations of the ligand within C
the protein binding site can be obtained. This information can also reflect the nature and
A
quality of the interaction. The proteins like caspase-8 (PDB Id: 1QDU), anti-apoptotic protein
L
Bcl-xL (PDB Id: 2O1Y), M20 family metallo peptidase (PDB Id: 2POK), caspase-3 (PDB Id: 2XZT)
T
which displayed superior binding interactions and which possessed highest inhibitory activity
among the various selected receptors. Here I appended the result of Caspase 8 protein E
interaction figure C
H
N
O
L
O
G
Y
caspase-8 (PDB Id: 1QDU)
77
IN VITRO ANTIOXIDANT ACTIVITIES, TOTAL FLAVONOIDS AND TOTAL PHENOLIC CONTENT P

OF ETHANOLIC EXTRACT FROM WHOLE PLANT OF LACTUCA RUNCINATA (DC). H
Jeyaraman Amutha Iswarya Devi1, Arumugam Kottai Muthu2
1,2
Department of Pharmacy, Annamalai University, Annamalai Nagar-608 002, Tamil Nadu, India.
A
R
To evaluate the in vitro antioxidant activities and to estimate total phenol and M
flavonoids of the crude ethanolic extract from the whole plant of Lactuca runcinata DC.The A
shade dried whole plant powder was extracted with ethanol by using soxhlet extractor and C
crude extract was used for in vitro antioxidant activity by DPPH, nitric oxide, iron chelating, E
hydroxyl radical, super oxide radical scavenging, total antioxidant and FRAP assay methods. U
Total phenols and flavonoids also estimated. The results revealed that L. runcinata ethanolic T
extract have higher antioxidant property and contains large amount of phenols and I
flavonoids. This study helps to predict it is a better source of natural antioxidant and contains C
large amount of phenols and flavonoids which can be used as drugs and further investigation A
may lead to the development of drug formulation. L
T
Keywords: Ethanolic extract, in vitro antioxidant, Lactuca runcinata DC. Total flavonoids.
E
C
H
S.No Concentration % of activity(±SEM)* % of activity(±SEM)*
N
(µg/ml) Sample Standard Sample
(Ethanolic (Rutin) (Ethanolic
Standard O
extract) extract)
(Ascorbate) L
1 125 21.64±0.049 18.85 ± 0.076 36.710.09 27.630.076 O
2 250 30.36±0.087 22.08 ± 0.054 45.520.17 49.53 0.054 G
3 500 48.54±0.069 52.21 ± 0.022 52.670.11 55.120.022 Y
4 1000 63.46±0.018 69.83 ± 0.014 57.850.09 62.000.014
IC50 = 510 IC50 = 480 IC50 = 425
IC50=410 g/ml
µg/ml µg/ml µg/ml
Effect of ethanolic extract of L. runcinata on DPPH assay& Nitric oxide free radical scavenging method
*All values are expressed as mean ± SEM for three determinations
78
THERAPEUTIC POTENTIAL OF PLEUROTUS OSTREATUS: AN EDIBLE MUSHROOM IN HUMAN P

CANCER CELL LINE (MCF-7) AND IN RAT MAMMARY CARCINOMA H
K.Deepalakshmi and S.Mirunalini
Department of Biochemistry and Biotechnology, Annamali University, Chidambaram.
A
Email id: mirunasankar@gmail.com, Mob: 9442424438 R
M
Objective of this study was to explore the chemotherapeutic efficacy of A
Pleurotus ostreatus ethanolic extract (POEet) against mammary cancer using in vitro and C
in vivo models. Preliminary phytochemical analysis of mushroom was used in folklore has E
yielded a number of compounds with various pharmacological activities. Thus in our study we U
first aimed to find out the active constituent (Ergosterol) in POEet by HPTLC fingerprint and T
I
quantified in POEet with reasonable accuracy in shorten time. In addition, POEet was
C
investigated in human mammary cancer cell line (MCF-7). As assessed from 3-(4, 5-
A
dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, greater cytotoxicity was
L
observed with a IC50 value of 1024.02 µg/ml. Further we extended our work in animal model T
also. In order to confirm the chemotherapeutic potential of POEet against 7, 12-dimethylbenz E
(a) antheracene (DMBA) induced mammary cancer on Sprague dawley rats. After the end of C
experimental period we investigate the lipid profile status, thus the correlation of lipids and H
lipoproteins has been associated with the risk of breast cancer. The elevated levels of total N
cholesterol, phospholipids, triglycerides and free fatty acids in plasma, liver and mammary O
tissues of DMBA induced cancer suffering animals. And the levels of very low density L
lipoprotein (VLDL) and low density lipoprotein (LDL) were increased and High density O
lipoprotein (HDL) levels were decreased in cancer suffering animals. Whereas, oral G
Y
administration of (600 mg/kg bwt) POEet were significantly reverted back to near normal level
when compared to cancer animals thereby reduced the cancerous risk. Taken together our
finding revealed the chemotherapeutic potential of POEet due to the presence of the active
compound ergosterol which may responsible for the major impact on human mammary
cancer.
79
A CALCIUM CHANNEL BLOCKER - AMLODIPINE ATTENUATES ACETIC ACID INDUCED P

ULCERATIVE COLITIS IN MICE H
Rajinikanth B, Venkatachalam VV
Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamilnadu - 608 002, India.
A
Email id: rajini_pharm@yahoo.co.in Mobile: 9942885353 R
M
The objective of this study is to investigate the effect of Amlodipine, a calcium channel A
blocker on acetic acid induced ulcerative colitis in Swiss Albino mice based on the hypothesis C
of calcium blocking in the colon leads to decrease the neuropeptides which is responsible for E
ulcerative colitis. 0.1 ml of (6% v/v) acetic acid (AA) was used to induce ulcerative colitis and U
assess clinical disease activity index (DAI) everyday like body weight loss, stool consistency T
and gross bleeding. After seven days treatment with Amlodipine blood was collected from I
retro orbital puncture, serum was separated and C - reactive protein (CRP), Total protein (TP) C
were also measured. Colon was excised, washed and its length measured and its tissue
A
homogenate was subjected to measure myeloperoxidase (MPO) assay, lipid peroxidation and
L
cytokines like IL-6 and TNF-α. Intracolonic administration of Amlodipine significantly reduced
T
the severity of AA induced ulcerative colitis. It diminished the DAI from the fourth day
onwards, which shown that it suppresses body weight loss, stool consistency and gross E
bleeding. Serum concentrations of CRP levels are reduced and TP levels also increased into C
normal levels. Amlodipine prevents tissue MPO accumulation, colon shortening, and lipid H
peroxidation effectively in inflammed colonic tissue and also reduced inflammatory cytokines N
like IL-6 and TNF-α. The present study results confirmed that the Amlodipine, a calcium O
channel blocker possess significant reduction in inflammation against acetic acid induced L
ulcerative colitis in mice. O
G
Y
Changes in disease activity index were evaluated daily throughout the 7-day experimental period. Values
are expressed as mean±SEM of three independent experiments. ### P<0.001 acetic acid colitis group
compared with the normal control group. AML (0.65mg/kg), AML (1.3mg/kg) and Prednisolone (5mg/kg)
shows significantly decrease DAI compared with the acetic acid induced colitis group
80
A SINGLE CHEMICAL ENTITY TO BALANCE ANTI-AMYLOID AND ANTI-CHOLINESTERASE P

CAPACITY: IN SILICO DRUG DESIGN H
Pavadai Parasuraman1, Ramalingam Suresh1, Manathusamy Gopalakrishnan2
1
Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamilnadu-608002, India
A
2
Department of Chemistry, Annamalai University, Annamalai Nagar, Chidambaram, Tamilnadu-608002, India R
EMail id: pvpram@gmail.com M
A
Alzheimer's disease is currently the deliberations to be a syndrome, multiple complex C
factors; it is unlikely to occur from a single causal factor; instead, there are a number of E
biological abnormalities allies believe that puts them into its pathogenesis. This may explain U
why the drugs currently available, developed according to the archetypal paradigm for drug
T
discovery “one-molecule-one-target," have proven palliative. In light of this, a combination of
I
medicines that may work with different levels of the neurotoxic cascade provides new
C
horizons in the direction to the treatment of Alzheimer's disease and other neurological
A
diseases. In such, a promising new strategy in the development of a chemical entity and one
can modify ultiple targets simultaneously. This has led to a new paradigm in medicinal L
chemistry, design and strategy “multi-target–directed ligand", which has already been T
successfully exploited both academic and industrial.4-Piperidinone fused with acetylcholine E
mimic moiety was designed and docked against AChE and BACE enzyme Using Schrodinger C
software. As a case study, we report here on Ethyl 2-(2, 6-bis(4-bromophenyl)-3-butyl-4- H
oxopiperidine-1-carboxamido)acetate (KBr 5), a new molecule developed following this N
strategy. The in-silico proﬁle of Ethyl 2-(2,6-bis(4-bromophenyl)-3-butyl-4-oxopiperidine-1- O
carboxamido)acetate demonstrates the suitability of the new strategy for obtaining L
innovative drug candidates for the treatment of neurodegenerative diseases as it inhibits O
both AChE and BACE enzyme through in silico studies. G
Y
Keywords: In Silico, Alzheimer’s disease, 4-Piperidone, Multi-targeted Drug Design.
81
ANTIOXIDANT ACTIVITY AND MOSQUITOCIDAL ACTIVITY OF ALLIUM SATIVUM OF P

KODAIKANAL HILLY AREAS H
M.Razia, K. Lavanya and B.Sri Shaila Devi
Department of Biotechnology, Mother Teresa Women’s University Kodaikanal – 624 102, TamilNadu
A
R
Qualitative analysis of phytochemical compounds was studied in the plant Allium M
sativum using methanolic extract. The following metabolites were presented namely tannins, A
saponin, flavonoids, phenols, glycosides, alkaloids and reducing sugar. The nature of C
components present in the methanolic extract of A. sativum was analysed by GCMS. These E
compounds have several activities which involved in health care. N,N'- U
Diacetylethylenediamine (0.3%), Diallyldisulphide (0.8%) have Antimicrobial, Anticancer, T
Cancer preventive, Diuretic Antiinflammatory, insecticide activity. 1,3-Dioxepin, 4,7-dihydro- I
(0.1%), Palmitic acid (2.02 %), 4-methoxy-1-(2-oxobut-3-enyl) azetidin-2-one (1.1 %), C
Ricinoleic acid (2.26 %) showed antimicrobial and anti-inflammatory activity, Cyclohexanone, A
2-ethyl-4-ethoxy- (0.1 %), 8-(Trimethylsilyl)-6-octenteneitrile (2.9 %), Proline, 3,4- L
didehydro(19.3 %), 4, Amino-3- methoxypyrazolo (25.8 %) respectively. FTIR spectroscopy can T
be used the presence of characteristic functional groups Carboxylic acids, amines, amides,
E
sulphur derivatives, polysaccharides, organic hydrocarbons, halogens were identified in FT-IR
C
analysis. Data of larvicidal activity of crude extract of A. sativum against Ae. Aegypti, and Cx.
H
quinquefasciatus and Cx. tritaeniorhynchus were presented in table. The present study
exhibits the mosquitocidal properties in the plant extract suggesting their use in mosquito N
population control. The mortality rate of larval instars of Ae.aegypti, Cx. quinquefasciatus and O
Cx. tritaeniorhynchus at 0.5% concentration was significantly higher (P< 0.05) than the L
mortality rates at 0.1%, 0.2%, 0.3% and 0.4% concentrations of crude plant extract at 24, 48 O
and 72 h of exposure. Higher mortality rate was also recorded at 72 h bioassays than those at G
24 h and 48 h. Therefore, these extracts could be used for further isolation and purification Y
of active compounds.
GC-MS analysis of Allium sativum using Methanolic extract
82
INVITRO ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF P

Sesbania grandifolia LINN LEAVES H
K. S. Sridevi Sangeetha* and S.Umamaheswari
Department of Pharmacology, Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai.
A
Email id: sangeethsb@gmail.com Mobile: +91 9884023926 R
M
Sesbania grandiflora Linn. Is an Indian medicinal plant commonly called as agathi A
which belongs to family Fabaceae? It is mostly cultivated in south and west India in the Ganga C
valley and in Bengal. Tradionally Sesbania grandiflora was used for colic disorder, jaundice, E
small-pox, antipyretic, leprosy, night blindness and gout. Various part of the plant showed U
hepatoprotective activity but in vitro hepatoprotective activity in ethanolic extract of the T
leaves have not been studied so far, the present study was designed to investigate the I
qualitative anlaysis of phytochemical constituents, in vitro antioxidant and invitro C
hepatoprotective activity. Sesbania grandiflora leaves are collected in Tambaram and
A
authenticated. Acetaminophen was used as hepatotoxicity inducing agent and it was carried
L
out by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay in Chang
T
liver cell line. The phytochemical analysis of the plant showed the presence of flavonoides,
saponins, tannins and steroids. The invitro antioxidant was confirmed by DPPH (1-diphenyl- E
2-picrylhydrazyl) free radical scavenging activity. The leaves were found to have dose C
dependent hepatoprotective activity in Chang liver cell line (Figure 1). The result of this study H
indicate the leaf extract of Sesbania grandiflora have good potentials against hepatic disease. N
O
L
Keywords: Agathi, sesbania, acetaminophene, Hepatoprotective O
G
Y
83
PHYTOCHEMICAL INVESTIGATION AND INVITRO ANTI DIABETIC ACTIVITY OF P

MYXOPYRUM SERRATULUM A. H. Hill H
K. Kavitha1*, K.Sujatha1, S.Manoharan2, Sundara Ramaprabhu3
1
Department of pharmaceutical chemistry, Sri Ramachandra University, Porur, Chennai
A
2
Department of Veterinary public health and Epidemiology, Veterinary College Research Institute, Orathanadu R
– 614625 3Alternative energy and nanotechnology laboratory, Department of physics, IIT madras, Chennai M
Email id: kr.kavitha00@gmail.com, Mob. 9715342965 A
C
Myxopyrum serratulum A. H. Hill belongs to the family Oleaceae is a large scandent
E
shrub, distributed throughout Kerala. The whole plant is widely used as asthma, fever, cough,
U
wounds and rheumatism. The aim of the present study is to investigate the inhibitory activity
T
of ethanolic extract of Myxopyrum serratulum on alpha amylase, alpha glucosidase and
phytochemical studies of whole plant extract. The phytochemical studies were carried out in I
terms of physicochemical standardization, fluorescent analysis, preliminary phytochemical C
screening and quantification of important active constituents present in the whole plant. The A
phytochemical study results showed that the Myxopyrum serratulum plant extract have rich L
amount of active constituents such as phenolic content (78.09 mg/g), flavanoid (51.2 mg/g), T
flavonol (39.71 mg/g) and tannin (86.25 mg/g). Postprandial hyperglycemia is an early defect E
in diabetes which increases risk of diabetes related complication like cardiovascular diseases. C
One important therapeutic strategy for decreasing the postprandial hyperglycemia is to H
inhibit the action of hydrolyzing enzymes like alpha amylase and alpha glucosidase. The N
present study was to screen the inhibitory activity of Myxopyrum serratulum by means of O
invitro studies. The invitro assay result suggests that the ethanol extract of plant exhibit dose L
dependent inhibitory activity on alpha amylase and alpha glucosidase enzymes.IC50 values O
were 435.02µg/ml and 688.61µg/ml for alpha amylase and alpha glucosidase respectively. G
Acarbose used as a reference drug.
Y
Invitro antidiabetic activity of

70 Myxopyrum serratulum
Percentage of inhibition
60
50
40
30
Glucosidase
20
amylase
10
0
15.62
500
1.95
7.81
125
250
62.5
1000
31.25
3.9
Concentration
. (a)Myxopyrum serratulum A. H. Hill flower, (b) Inhibitory activity of Myxopyrum serratulum on α-amylase
and α-glucosidase enzymes
84
ENERGY CONSERVATION OPPORTUNITIES IN PHARMACEUTICAL AND PAPER PLANT P

INDUSTRIES USING COMBINED HEAT AND POWER H
N.stalin
A
Assistant Professor, Department of Petrochemical Technology, Anna University, BIT Campus,
Tiruchirappalli - 620024, TN Email: mnstalin@gmail.com, Mob: 9976577636 R
M
Energy crisis and the exponential hikes in the cost of energy, the energy audit is of A
prime importance in all industrial sectors. Energy audit paves the way to understand the areas C
where energy and fuel are consumed in the industry, identify the areas where wastage of E
energy can occur and where the scope of improvements exists. Energy audit in a nutshell U
quantifies how and where energy is being spent in industries. The pharmaceutical and paper T
industry is one of the most energy intensive industries in the manufacturing sector. The I
energy consumed by the utilities alone would be over 50% of the total energy requirement of C
the site. Energy audit of a pharmaceutical industries will improve the cost effective operation A
in manufacturing the products and paper plant in which the raw material used is old
L
corrugated paper, used newsprint, Kraft waste and used paper boards. Overall energy
T
consumption and the specific energy (both electrical and thermal) consumption are also
E
calculated and compared with the Standard SEC. The performance analysis of major energy
C
utilizing equipments is also done comprehensively and their operating equipment efficiency
is determined so as to undertake some improvements or replacing the whole equipment H
itself. The present turbine is analyzed for its optimum use and proposed a new turbine to N
increase the power output. In addition to that some of the Energy conservation proposals are O
proposed to reduce Energy consumption and increase cost savings. Each and every Energy L
conservation proposals is discussed in depth along with detailed backup calculations and O
simple payback Analysis. G
Y
Keywords: Energy Conservation, Pharmaceutical Plant, Paper Plant, CHP, Operating
Efficiency.
85
ANTIOXIDANT PROPERTIES OF LAGENARIA SICERARIA FRUIT JUICE BAGASSE OBTAINED AS P

A CO-PRODUCT BY CHEMICAL & ELECTROCHEMICAL ASSAYS H
Sameer D Kulkarni, Barij N Sinha, K Jayaram Kumar *
Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi-835215,
A
Jharkhand, India. Email id: jayarampharm@gmail.com R
M
In India, Lagenaria siceraria fruit juice has been widely used as a cardioprotective and A
claimed to have various other health benefits. The aim of the present work was to assess the C
antioxidant potential of L. siceraria fruit bagasses, which was obtained in two distinct ways E
viz., direct extraction that involved whole fruit and from peels. Four different assessment U
methods were used, namely DPPH radical scavenging activity, the ferric reducing antioxidant T
power (FRAP); the ferrous ion-chelating ability (FIC) and the cyclic voltammetric technique I
were employed for the determination of antioxidant activity. Electrochemical analyzer C
equipped with a glassy carbon working electrode operating voltammetrically at a potential of
A
0. 5V (vs. Ag/AgCl) was used for the purpose. A good correlation between these four methods
L
was found. The peel samples showed higher antioxidant activity than whole fruit bagasse. The
T
total phenol contents (TPC), the total flavonoid (TFC), the tannin content (TC), the total
carbohydrate contents (TCC) were also determined. Peel bagasse presented higher total E
phenolic, flavonoid and tannins contents (9.85 mg gallic acid equivalent/g sample; 6.36 mg C
rutin equivalent/g sample and 5.47 mg catechin/g sample) than fruit bagasses. H
N
Keywords: Lagenaria siceraria, peel bagasse, whole fruit bagasse, antioxidant activity O
L
O
G
Y
86
GC-MS CHARACTERIZATION OF VOLATILE ODOROUS COMPOUNDS IN ALLIUM CEPA P

N.C.J.Packia Lekshmi1, S.Viveka2, M.B.Viswanathan3, G.Manivannan4, and T.Mini Shobi3
1 H
Department of Biotechnology, Sathyabama University, Solinganallur, Chennai 600119, Tamil Nadu, India.
2
Department of Biotechnology, Udaya School of Engineering, Vellamodi, Kanniyakumari 629204, Tamil Nadu, A
India. 3Department of Plant Science, Bharathidhasan University, Tiruchirappalli 620024, Tamil Nadu, India R
4
Department of Microbiology, NMSSVN College, Nagamalai, Madurai 625019, Tamil Nadu, India. M
Email Id: packia_3779@yahoo.co.in
A
C
An analytical technique for the determination of volatile phytochemical compounds
by GC-MS in onion, Allium cepa L., was reported here. Three different cultivars of onion bulb E
(O1, O2 and O3) were extracted with petroleum ether and the volatile compounds were U
analyzed by GC-MS. Terpenes such as humulane and phytosterols and aldehydes are found T
common in all the three cultivars. Thiophenes and disulphides were identified as major I
compounds in O1 and O3 respectively. Caryophyllene, a terpene was observed in both O1 C
and O2. Squalene, allicin and sulphide compounds were identified specifically in O3. The A
study further reported that the composition of volatile compounds in the cultivars is varied L
and these differences may be due to the variations in geographic, climatic, soil fertility and T
cultivation practices. The intensity of flavour is governed by hereditary factors and E
environmental conditions under which the onions grow. C
H
Keywords: Phytochemicals, GC-MS, onion, Terpenes, Disulphides, N
O
L
O
G
Y
87
ISOLATION & EVALUATION OF CYPERUS ROTUNDUS RHIZOMES STARCH GRANULES FROM P

JHARKHAND, INDIA AS A PHARMACEUTICAL ADJUVANT H
N. Paramakrishnan S. Jha, K. Jayaram Kumar
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi-835215,
A
Jharkhand, India. Tel.: +91 06512276247; fax: +91 06512275290. E-mail: jayarampharm@gmail.com R
M
Starch granules were isolated from Cyperus rotundus (CRSG) rhizomes of the A
Jharkhand region, India. The physicochemical and functional property of the starch granules C
was assessed in order to utilize its potential as a pharmaceutical adjuvant. Rhizomes of E
Cyperus rotundus yielded a marked amount of starch granules i.e., 29.19 % w/w. The starch U
was characterized for its distinct physicochemical properties including amylose & amylopectin T
contents, moisture content, ash value, solubility, water swelling and water retaining capacity. I
Scanning electron micrographs revealed the smooth granules' surface of the starch. The C
granules were found to be spherical, mostly round, disc like, flat and the size range was 3.21-
A
14.25 µm. The pH of isolated starch granules was found to be near to the neutral. The
L
solubility and swelling power was found to be increased with the increase in the temperature.
T
The thermogravimetric curve of CRSG revealed that the starch is thermally stable with less
bound water. The Finger print region of FT-IR spectra confirmed its polysaccharide nature. E
The micromeritic properties of isolated granule's bulk density, tapped density, Carr’s index, C
Hausner ratio, true density and porosity found unique practicability of being used as an H
excipient in pharmaceutical solid dosage forms. Tablets prepared by using CRSG showed high N
mechanical strength, elastic recovery and more disintegration time, which illustrates the O
characteristic binding nature of the starch. L
O
G
Y
88
SYNTHESIS OF NOVEL 1, 2- DIAZOLES AND EVALUATION OF THEIR ANTICANCER ACTIVITY. P

Amruta Shukla, R.S.Jeyaprakash
H
Department of Pharmaceutical Chemistry.
Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104. A
R
1, 2- diazoles were found to have varied biological activities such as antibacterial, M
antifungal, anti-inflammatory, antihistaminic, anticancer and anticonvulsant activities. In the A
present work a series of novel 1, 2- diazole derivatives were synthesized and characterized by C
mass, IR, 1H NMR and 13C NMR spectroscopy techniques. The synthesized compounds were E
evaluated for their in vitro anticancer activity. The anticancer activity was performed on MCF- U
7 cell line by MTT assay. Compounds DA-3, DA-4, DA-8 and DA-9 showed considerable activity
T
with IC50 values of 44, 37, 51.87 and 17 µg/ml respectively. It was observed that compound
I
DA-9 showed promising in vitro anticancer activity against MCF-7 cell line. Doxorubicin was
C
used as the standard.
A
Keywords: 1, 2-diazoles, MTT assay. L
T
E
S. No Comp Code IC50 (µg/ml) S. No Comp Code IC50 (µg/ml)
C
1 DA-1 >100 6 DA-6 >100
H
2 DA-2 >100 7 DA-7 >100
N
3 DA-3 44 8 DA-8 51.87
O
4 DA-4 37 9 DA-9 17
L
5 DA-5 >100 10 DA-10 >100 O
G
IC50 values of MTT assay
Y
89
SCREENING OF AEGLE MARMELOS FOR ANTIDIABETIC ACTIVITY IN STREPTOZOTOCIN P

INDUCED DIABETIC RATS H
S. Jebaseelan, Dr. P. Ramasubramanian, Dr. Venkatesh
Professor, Department of Pharmaceutical Chemistry, Ultra College of Pharmacy, Madurai, Tamil Nadu, India
A
Professor, Dept. of Pharmaceutics, Sankaralingam Bhuvaneshwari College of Pharmacy, Sivakasi, Tamil Nadu, R
India Professor, Dept. of Pharmaceutical Chemistry, Sankaralingam Bhuvaneshwari College of Pharmacy, M
Sivakasi, Tamil Nadu, India Email id: jebaseelanmpharm2000@rediffmail.com A
C
E
Diabetes is a chronic metabolic disorder that has emerged as one of the main alarms
U
to human health in the 21st century. Many allopathic medicines are available to treat but
T
treatment is associated with many side-effects which necessitates the replacement of
allopathic medicine with natural drug. As an alternative medicine to treat diabetes mellitus, I
many herbal drugs are being studied throughout the world. In the present study an attempt C
was made to investigate the anti-diabetic activity of Aegle marmelos in streptozotocin A
induced diabetic the study was conducted among five groups with 3 albino rats in each group. L
The ethanolic and aqueous extracts were administered for 7 days. The plasma glucose T
concentration significantly decreased by both the extracts when compared with the control. E
The study shows that the ethanolic and aqueous extracts of Aegle marmelos has anti C
hyperglycemic activity, is able to ameliorate the diabetic state and is probably a source of H
hyoigkycemic compound. N
O
Keywords: Aegle marmelos, diabetes, streptozotocin, extracts L
O
G
Y
90
BIOSYNTHESIS OF SILVER NANOPARTICLES USING TEPHROSIA TINCTORIA FOR P

ANTIBACTERIAL ACTIVITY AGAINST MULTIDRUG RESISTANT PATHOGENS H
D.C. Aiswaryaa, K. Rajaramb, P. Sureshkumarb*
a
Department of Nanoscience and Nanotechnology, SRM University, Kattankulathur - 603 203, Chennai, Tamil
A
Nadu, India bDepartment of Biotechnology, Bharathidasan Institute of Technology, Anna University, R
Tiruchirappalli-620 024, Tamil nadu, India. M
Email id: drsureshbiotech2003@gmail.com; A
C
The aqueous extract of T. Tinctoria was dissolved with different concentration of silver
E
nitrate and allowed for 24hrs. Light yellow colour of solution was changed into brown colour.
U
Because, the secondary metabolites was present in the plant extract reduces the silver
T
nitrate. All reaction mixture were analysed under UV spectrophotometer. The peak between
400-500nm was increased when increase the concentration of silver nitrate. Then the I
reaction mixture was centrifuged and washed with distilled water. Further it was C
characterized using TEM and FTIR. The green synthesized AgNPs was showed higher inhibitory A
activity in the multidrug resistant pathogens (E.coli, P.aeruginosa, K.pneumoniae, E.faecalis, L
P.vulgaris and S.aureus). T
E
Keywords: Tephrosia tinctoria, AgNPs, TEM, Antibacterial agent and FTIR C
H
N
O
L
O
G
Y
Antibacterial activity of AgNPs against clinical pathogens (A) P.aeruginosa,(B) K.pneumoniae, (C) S.aureus
(D) E.faecalis, (E) E.coli and (F) P.vulgaris. (Disc Diffusion Method)
91
AN EFFICIENT SYNTHESIS OF DIHYDROPYRIMIDINONE DERIVATIVES USING AMMONIUM P

CHLORIDE-CATALYZED UNDER NEAT CONDITIONS FOR BIGINELLI TYPE REACTION. H
M.Jayanthi1*, P.Venkatesh2, S.V.Thirunavukkarasu3*.
1,2 Department of Chemistry, Pachaiyappa’s college, Chennai-600 030, India. A
3
Department of Medical Cyclotron, Health Care Global, Dr.KMH Campus, Chennai – 600 100. R
M
An efficient synthesis of dihydro pyrimidinones from aldehyde, ethyl aceto A
acetate and urea (thiourea) under solvent-free conditions, using ammonium chloride C
as a catalyst. This methodology significance due to use of Ammonium chloride as an E
affordable and readily accessible reagent, without use of any organic solvent and toxic U
metals as catalyst and thus reduces the cost of preparation for Pyrimidine derivatives T
in cancer treatment, operational hazards and also not affect environmental. This I
modified methodology provides much higher yields and easy work-up procedure of C
the products as compared with classical Biginelli reaction. A
L
Keywords: Ammonium Chloride; Solvent free condition; Green Application; T
Heterogeneous catalyst.
E
C
H
N
O
L
O
G
Y
92
CHARACTERIZATION OF SOLID STATE FORMS OF PIOGLITAZONE P

B.Poornima1, K.Bharathi2
H
Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Vishwa Vidyalayam, Tirupathi, Andhra Pradesh,
Email id: poornimachandran7@gmail.com Mobile No: 08790355248 A
R
Pioglitazone hydrochloride is a novel antidiabetic drug of thiazolidinedione’s group M
and it improves insulin sensitivity in insulin resistant patients. It is having low solubility and A
high permeability. The discovery of new polymorphic forms of Pioglitazone provides new C
opportunities to improve the characteristics of the active pharmaceutical ingredient. So, the E
present study related to preparation and characterization of different polymorphic forms of U
Pioglitazone. Till the date, two polymorphs of Pioglitazone (Form I & Form II) were reported. T
The present study is an attempt to enhance the bioavailability of pioglitazone HCl by I
developing its polymorphic forms using different techniques like anti solvent method, solvent C
diffusion method, solvent evaporation method and solvent grinding method, cooling method,
A
slurry method and neat grinding method. The commercial form used for the study was
L
characterized Form I. Form B obtained from anti solvent method, is similar as that of
T
reported Form II of Pioglitazone indicating that polymorphic transition from commercial form.
The prepared polymorphic forms were characterized for flow properties like bulk density, E
tapped density, Carr’s Index, Hausner’s ratio, angle of repose, in vitro drug dissolution and C
also by various spectral methods like Fourier Transform Infrared Spectroscopy (FTIR), H
Scanning Electron microscopy (SEM), Differential Scanning Calorimetry (DSC) and X-ray N
diffraction. The other forms like Form E, D, C, and S had different X-ray diffraction patterns O
and very different thermal behaviour. Among all the forms Form E& G had better dissolution L
properties than the commercial form. O
G
120 Y
In vitro dissolution of Pioglitazone Polymorphs

100
80 Form E
Form D
60 Commercial form
Form S
40 Form G
Form C
20
0
0 50 100 150 200
. In vitro dissolution of Pioglitazone polymorphs
93
FLAVONOIDS AND THEIR MEDICINAL SIGNIFICANCE – AN UPDATE P

K.S. Sridevi Sangeetha and S.Umamaheswari
H
Department of Pharmacology, Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai.
Email id: sangeethsb@gmail.com Mobile: + 91 9884023926 A
R
Flavonoids are natural compounds widely distributed in plant kingdom, responsible M
for the various colors exhibited by bark, leaves, flowers, fruits and seeds of plants. They are A
the secondary metabolites of plant with significant antioxidant properties. Apart from the C
anti-oxidant potential, flavonoids have sedative, antidepressant, anticonvulsant, anti- E
proliferative, anti-inflammatory, anti-microbial, cardioprotective, antiulcerogenic, U
antidiabetic, hepatoprotective and antihypertensive activities. Many researchers have T
revealed that flavonoids have effect on mammalian enzymes like protein kinases, alpha- I
glucosidase and aldose reductase, thereby regulating multiple cellular signaling pathways that C
were altered during disease condition. The present review is focused on the classification,
A
structure, metabolism and pharmacological actions of flavonoids. The literature selected for
L
this review was obtained through database and internet search engines and domains. All the
T
information provided was collected from peer reviewed published literature, technical
reports, documentations, chemical s and books. E
C
Keywords: Flavonoids, antioxidant, pharmacological action H
N
O
L
O
G
Y
94
GUAR GUM: A FORMULATOR'S PRIDE P

Amisha Vyas, Dr.SaurabhDahiya H
School of Pharmaceutical Sciences,
A
Apeejay Stya University, Gurgaon, Haryana, India. R
M
Guar also known as cluster bean is the source of a natural hydrocolloid,
A
(Cyanopsistetragonoloba)which is cold water soluble and form thick solution at low
C
concentrations. The guar seed consists of three parts: the seed coat (14-17%), the endosperm
E
(35-42%), and the germ (43-47%). Guar is an important source of nutrition to both human
beings and animals.The guar industry has evolved from being used for domestic and raminant U
feed purpose, finding used in industry. Owing to new technology, research and development T
being done in this field, the natural gum property has found varied applications from food, I
pharma industry to oil industry. Due to fascinating properties they are used as a rheological C
modifier in food, pharmaceutical, paper, textile, oil and drilling and score the other industrial A
and commercial sectors. Guar gum is an important natural hydrocolloids. In order to enhance L
its applicability in the industries, itsderivatization can be carried out to get novel derivatives T
with desired properties.Therapeutically it is used as hypoglycemic, hypolipidemic, E
antimicrobial, antiproliferative, appetite suppressant, bulk forming laxative in collitis and C
crohn's disease. The present review highlights the total over-view from the plant to H
production to the application of this wonderful herb in nano formulations. N
O
L
O
G
Y
95
PHARMACEUTICAL INDUSTRIES ENERGY MANAGEMENT AND INTEGRATION OF PEM FUEL P

CELL WITH UAV H
N.Stalin
A
Assistant Professor, Department of Petrochemical Technology, Anna University, BIT Campus, Tiruchirappalli -
620024, TN. Email: mnstalin@gmail.com, Mob: 9976577636 R
M
The main objective of this study is to replace PEM Fuel Cell as an alternative energy A
source to Li batteries to power up Unmanned Aerial Vehicle and pharmaceutical industry C
applications. The author has developed a new software tool using Visual Basic to identify the E
performance of PEM Fuel cell using graph obtained from tested data. The Compatibility U
analysis made in this paper and other research and development related with PEM fuel Cell T
were analyzed. Hydrogen Generator using sodium boro hydride which is compatible with 100 I
Watts PEM fuel cell design and development were analyzed in detail. 200 Watts PEM fuel Cell C
which is Compatible with UAV design, structure and its performance were studied. Mass and A
energy balance of PEM fuel cell are calculated. The possibility of static power generation using
L
fuel cell was explained and other recent technological development in PEM Fuel Cell and its
T
benefits was explained.
E
C
Keywords: PEM Fuel Cell, Pharmaceutical Industries, Li batteries, Hydrogen Generator, UAV
design. H
N
O
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O
G
Y
96
A REVIEW ON ANIMAL MODELS INVOLVED DRUG DISCOVERY PIPELINE FOR THE P

ALZHEIMER DISEASE H
Benaseer Begam R, Dhivya P.S, Selvamani P, Latha S
Anna University, BIT campus, Tiruchirappali-620 024
A
Email: lathasuba@yahoo.co.in Mob: 9842598097 R
M
Alzheimer’s disease is the most common cause of memory impairment and dementia, A
which occurs equally both genders. German physician Aloe’s Alzheimer first described the C
progressive neurological disease of brain named Alzheimer’s disease in 1906.There is no E
animal model available that can mimic all the cognitive, behavioral, biochemical, and U
histopathological abnormalities observed in patients with AD. However, partial reproduction
T
of Alzheimer’s disease neuropathology and cognitive deficits has been achieved with
I
pharmacological and genetic approaches. Cholinergic dysfunction-related animal was used to
C
assess the effect of propentofylline, which was shown to slow the progression of Alzheimer’s
A
disease in phase clinical trials and its prevented memory impairment and decrease in
acetylcholinesterase activities in the hippocampus. Amyloid -Peptide related animal models L
for Alzheimer disease are divided into transgenic and non-transgenic animals. Transgenic T
animals to generate Alzheimer’s disease animal models exhibiting senile plaques and Aβ – E
associated neuropathology, different types of transgenic mice that express human APP and C
non-transgenic animal models, which growing number of studies have demonstrated that H
acute injection or continuous infusion of Aβ into the brain cause brain dysfunction, as N
evidenced by neurodegeneration and an impairment of learning and memory. In O
antioxidants, oxidative stress is important in the pathogenesis of Alzheimer’s disease, and it L
protect neurons from Aβ-induced toxicity and Epidermiological studies have indicated that O
the prevalence of Alzheimer’s disease after age 65 is two to three times higher in women than G
men, suggesting gender difference as a risk factor of Alzheimer’s disease. In anti-amyliod β- Y
Peptides agent, the effects of β-and secretes inhibitors on Aβ production and deposition in
vivo should be examined with Aβ- related transgenic animals models, not the nontrasgenic
animal models. We have reported that repeated daily administration of numerous types of
cognition enhancers improve learning and memory and impairments. Significant progress has
been during the last decade in understanding the neuropathology and mechanism of the
biochemical and cellular abnormalities and in identifying genes implicated in Alzheimer’s
disease. In parallel, numerous kinds of nontransgenic and transgenic animal models for
Alzheimer’s disease have been developed. We believe that investigation of currently available
transgenic and nontransgenic animal models for Alzheimer’ disease will help to clarify the
pathogenic mechanism and allow assessment of the effects of new treatment strategies.
97
A SCENARIO OF NATURAL PLANT RESOURCES ON BREAST CANCER THERAPY P

R. Jayalakshmi, M. Ponmalar, P. Selvamani and S. Latha
H
Department of Pharmaceutical Technology, Anna University, BIT Campus, Tiruchirappalli – 620 024, Tamil
Nadu, India A
Email id: rjayalakshmi.au@gmail.com R
M
Breast cancer is the most common cancer worldwide, comprising 23% of all the female A
cancers and the second most leading cause of cancer deaths in women today. It is the most C
frequent cancer in both developed and developing countries and it occurs in one out of eight E
women during her lifetime. Breast cancer can be benign and malignant and can also affect U
the bone, lungs, regional lymph nodes, liver and brain, with the most common site being the
T
bone. The existing therapeutic agents for breast cancer have certain limitations and also have
I
substantial side effects. So there has been an intense search on various biological sources to
C
develop a novel anti- breast cancer drug leads to compact the breast cancer. Natural sources
A
are being the rich reservoir of cancer therapeutic agents, numerous new anticancer agents
were identified from natural sources like plant, marine organisms, microorganisms, L
vegetables and fruits etc,. At present over 60% of currently used anticancer agents are derived T
in one way or another from natural sources, including plants. Even the FDA approved 60-75 E
% anticancer and anti-infectious drugs are the natural origin. About 30 plant derived C
compounds have been isolated so far and are currently under clinical trials. So the plant H
derived compounds will be an important natural source for anti-cancer/anti-breast cancer N
therapy. O
L
Keywords: Breast cancer, cancer therapy, natural compounds and anti breast cancer agent. O
G
Y
98
DEVELOPMENT OF VALIDATED ANALYTICAL METHOD FOR ESOMEPRAZOLE AND P

DOMPERIDONE IN PHARMACEUTICAL SOLID DOSAGE FORM BY HPTLC H
Sunil singh1*, Rajiv dahiya2
1
Research Scholar, Department of Pharmacy, Mewar University, Chittorgarh, Rajasthan
A
2
Department of Pharmaceutical Chemistry, Globus College of Pharmacy, Bhopal (MP) R
rssunil29@rediffmail.com M
A
A simple, sensitive, precise, accurate and specific high performance thin layer C
chromatographic (HPTLC) method has been developed and validated for estimation of E
esomeprazole and domperidonein combined solid dosage form. The stationary phase used U
was precoated silica gel 60F254 plate. The mobile phase used was Acetonitrile: Triethylamine
T
(5:0.6 v/v). The detection of spots was carried out densitometrically using a UV detector at
I
290 nm in absorbance mode. This system was found to give compact spots for esomeprazole
C
(Rf value of 0.65± 0.03) and for domperidone (Rf value of 0.75± 0.04). The method was
A
validated in terms of linearity, accuracy, precision, limit of detection, limit of quantification
and specificity according to the International Conference on Harmonization guidelines. The L
calibration curve was found to be linear between 100-700ng/spot for esomeprazole and 100- T
600 for domperidone, with significantly high value of correlation coefficient (r 2> 0.99). The E
percent average recoveries obtained were 98.95±0.32 for esomeprazole and 99.58±0.22 for C
domperidone. The limits of detection and quantitation were found to be 45.31ng/spot and H
65.11ng/spot for esomeprazole and 54.41 ng/spot and 87.21ng/spot for domperidone. The N
developed method was successfully used for the assay of esomeprazole and domperidonefor O
solid dosage form without interferences of excipients. The proposed method was found to be L
repeatable. O
G
Keywords: Estimation; HTPLC; ICH; Esomeprazole; Domperidone Y
99
SYNTHETIC AND PHARMACOLOGICAL STUDIES ON A NATURAL CYCLOOLIGOPEPTIDE P

Rajiv Dhiya1, Sukhbir Lal khokra2, Suresh kumar3
1 H
Department of Pharmaceutical Chemistry, Globus College of Pharmacy, Bhopal, Madhya Pradesh
2
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana A
3
Research Scholar, Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana R
sureshmpharma@rediffmail.com M
A
Present work reports the first total synthesis of a phenylalanine-rich peptide –
C
dianthin F, previously isolated from extracts of Dianthus superbus, by coupling of dipeptides
E
Boc-Gly-L-Pro-OH and tetrapeptide L-Phe-L-Val-OMe, followed by coupling of resulting
U
tetrapeptide unit with L-Phe-OMe.HCl usinf solution phase technique. Finally, linear
T
pentapeptide unit was cyclized to form desired cyclic pentapeptide. The chemical structure
of the compound was elucidated by FTIR, 1H NMR, 13C NMR, FAB MS spectral data and I
elemental analyses. The newly synthesized peptide was subjected to biological screening and C
found to possess good cytotoxicity against Dalton’s lymphoma ascites (DLA) and Ehrlich’s A
ascites carcinoma (EAC) cell lines, in addition to moderate anthelmintic activity against L
earthworms M. konkanensis, P. corethruses and Eudrilus sp. at a dose of 2 mg/ml. T
E
Keywords: dianthin F; cyclic peptide; solution phase synthesis; biological screening. C
H
N
O
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O
G
Y
100
SYNTHETIC AND BIOLOGICAL EVALUATION OF A NATURAL PROLINE-RICH PEPTIDE P

Rajiv Dahiya
H
Department of Pharmaceutical Chemistry, Globus College of Pharmacy, Bhopal, Madhya Pradesh
drrajivdahiya@gmail.com A
R
In present study, it was of interest to synthesize a natural proline-rich M
cyclooligopeptide - cyclomontanin D by coupling of tripeptide Boc-L-Asn(bzh)-L-Pro-Gly-OH A
and tetrapeptide L-Leu-L-Pro-L-Tyr-L-Ala-OMe followed by cyclization of linear polypeptide C
fragment. Structure of synthesized cyclopeptide was confirmed by detailed spectral analysis E
including FTIR, 1H NMR, 13C NMR, ESIMS/MS and elemental analysis. From the results of U
biological evaluation, it was concluded that newly synthesized cyclooligopeptide possessed T
good bioactivity against Gram-negative bacteria Klebsiella pneumonia, Pseudomonas I
aeruginosa as well as potent antidermatophyte activity against Microsporum audouinii and C
Trichophyton mentagrophytes, in comparison to reference drugs - ciprofloxacin and
A
griseofulvin. Moreover, synthesized peptide exhibited moderate antifungal activity against
L
pathogenic Candida albicans with MIC value of 6 g/ml.
T
E
Keywords: cyclomontanin D; cyclic peptide; solution phase synthesis; biological activity.
C
H
N
O
L
O
G
Y
101
COMPARATIVE ANALYSIS OF TOTAL QUERCETIN CONTENT IN AQUEOUS AND EHANOLIC P

EXTRACT OF ARTOCARPUS HETEROPHYLLUS BY LIQUID CHROMATOGRAPHY H
Jyoti Shrivastava, Sarvesh Paliwal
Department of Pharmacy, Banasthali University, Rajesthan.
A
R
Bio-flavonoids are now-a-days regarded as promising and significantly attractive M
natural substances to enrich the current therapy options against diabetes. Quercetin is a A
naturally occurring flavonoid which is a polar auxin transport inhibitor and also induce insulin C
secretion by activation of L-type calcium channels in the pancreatic β-cells. Due to the E
presence of quercetein, the plant is supposed to have high antidibetic activity that may be U
the reason of high medicinal potential of plant. The present investigation deals with the T
comparison of total quercetin content in aqueous and alcoholic extract of Artocarpus I
Heterophyllus. The quercetin content was observed 7.9 % in aqueous extract and 18.4 % in C
ehanolic extract of Artocarpus Heterophyllus. A
L
T
E
C
H
N
O
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O
G
Y
102
DENDRIMERS BASED TARGETED DRUG DELIVERY OF RIVASTIGMINE FOR TREATMENT OF P

ALZHEIMER’S DISEASE H
C.Sankar, P.Prasanth and G.Krishna reddy
Drug delivery systems laboratory, KMCH College of Pharmacy, Coimbatore
A
drcsankar@rediffmail.com R
M
Dendrimers were prepared and used for incorporation of rivastigmine-PAMAM A
(polyamidoamine) complex (4:1) ratio. And characterization studies like viscosity, infra red C
spectroscopy, Dynamic light scattering calorimetry, Nuclear magnetic resonance E
spectroscopy and toxicological studies were performed. From these studies 1.5 and 2.5G are U
selected for further studies.Dynamic light scattering calorimetry and invitro studies 1.5G of T
0.01M were showing as a best formulation and efficient one, in terms of smaller size and I
prolongation of drug release. Which may be beneficial for brain targeting. C
A
Key words: Alzheimer’s, dendrimer, Rivastigmine (exelon), PAMAM (poly amido amine).
L
T
E
C
H
N
O
L
O
G
Y
103
COMPUTATIONAL GRAPHICAL USER INTERFACE TOOL DEVELOPMENT FOR PERSONALIZED P

DRUG SELECTIVITY AND DESIGNED PHARMACOLOGY H
M. Poornima, P. Selvamani and S. Latha
Department of Pharmaceutical Technology, Anna University, BIT Campus, Tiruchirappalli
A
Email id: pselvamani@hotmail.com R
M
For a given drug product, it is of interest to study how the drug moves through the A
body and the processes of movement such as absorption (A), distribution (D), metabolism C
(M), and excretion (E) after drug administration. The key concept of a pharmacokinetic (PK) E
study is to study what the body does to the drug (which is usually characterized by ADME of U
a drug product after administration), while the key concept of a pharmacodynamic (PD) study T
is to study what the drug does to the body. This leads to the study of PK or population PK. The I
goal of a PK/PD study is to study the relationship between dose and response, which provides C
insightful information regarding: (i) how best to choose doses at which to evaluate a drug, (ii)
A
how best to use a drug in a population, and (iii) how best to use a drug to treat individual
L
patients or subpopulations of patients. Clinically, it may be necessary to apply
T
pharmacokinetic principles to determine dosing regimens for individual patients when one or
more of the following criteria are met: (i) A drug has a narrow therapeutic range, and serious E
clinical consequences result when the plasma concentrations are outside the range. (ii) A drug C
displays wide interpatient variability in its pharmacokinetic parameters. (iii) A patient H
possesses a characteristic that is frequently associated with altered pharmacokinetics. This N
may include renal disease, hepatic disease, and low activity of a drug-metabolizing enzyme O
due to genetic factors or concomitant medications, and low transporter activity.Drugs that L
are commonly subject to pharmacokinetic-based dosage individualization include O
aminoglycosides, phenytoin, lithium, immunosuppressants, and digoxin. This article G
implements part of the proposed Graphical User Interface (GUI) tool programmed using html Y
and javascript integrating the reported mathematical models for PK parameters for
personalized drug selectivity and to tailor real time dosage regimen.The object oriented
language allows to create modular programs with reusable code
Keywords: Pharmacokinetics, Pharmacodynamics, Individualization, Drug
Screen shot of Graphical User Interface
104
EXTRACTION & CHARACTERIZATION (BOTH PHYSICO-CHEMICAL & ANALYTICAL) OF PECTIN D

OBTAINED FROM DILLENIA INDICA & ABELMOSCHUS ESCULENTUS AND COMPATIBILITY R
STUDY WITH GLIPIZIDE IN NOVEL DRUG DELIVERY SYSTEMS
U
Sivasankar Mohanty1, G.Krishna Mohan2, M. Sunitha Reddy3.
Centre for Pharmaceutical Sciences, Institute of Science & Technology, Jawaharlal Nehru Technological G
University, Hyderabad, 500085. D
Email id : smohanty55@gmail.com. Mob: 7569310018 E
L
The present study was focused on extraction of pectin from Dillenia indica (DI) & I
Abelmoschus esculentus (AE) and characterized for establishment of polymer in place of V
synthetic or semi-synthetic in novel drug delivery systems. The Dillenia indica is known as
E
elephant apple belongs to family dilleniaceae and abelmoschus esculentus is known as ladies
R
finger belongs to family malvaceae. According to literature the pectin was extracted from
Y
different fruits like apple, citrus, pomace, Orange etc. Keeping in view the aim, the natural
S
mucilaginous substances were collected from DI, AE by Acetone precipitation method and
were undergone characterization like physico-chemical test, analytical study and Y
compatibility study with glipizide. The physico-chemical characterization were includes ash S
value, extractive value, lod and florescence analysis to know the purity of the pectin. The T
pectin was confirmed by different identification test like Stiff gel Test, Test with 95% Ethanol, E
Test with Potassium Hydroxide (KOH) & Iodine test and further quantification have done by M
carbazole test. The analytical study includes TGA, XRD, FTIR, DSC & HPTLC and also the zeta S
potential of the mucilage was studied using Zeta potentiometer, Malvern instrument. The
nature of pectin was confirmed by phytochemical screening and with Thin Layer
chromatography (TLC), and High Performance Thin Layer chromatography (HPTLC) which
indicated distinct finger prints with methanol extract, chloroform extract, acetic acid extract
& petroleum ether extract. The outcome of results has come within the limits according to
standard monograph. The compatibility study represents there was no interaction between
the drug (glipizide) and pectin. The swelling properties and mucoadhesion properties of the
mucilage were determined in-vitro and it was found that the mucilage had high swelling
capacities along with appreciable mucoadhesive strength. Moreover from the physico-
chemical & analytical studies.Thus the mucilage obtained from Dillenia indica & Abelmoschus
esculentus can be used as a natural mucoadhesive polymer for formulating various drug
delivery systems.
HPTLC of pure pectin HPTLC of pectin from DI HPTLC of pectin from AE
105
DESIGN AND IN-VITRO EVALUATION OF GLICLAZIDE LOADED TRANSDERMAL DRUG D

DELIVERY THERAPEUTIC SYSTEM CONTAINING HPMC AND PVP R
Raghuraman V. and V.P.Pandey
Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University,
U
Annamalai Nagar-608 002. Tamil Nadu, India. G
Email id: indramvk7@gmail.com. D
E
A novel monolithic matrix controlled release transdermal drug delivery system (TDDS) L
of Gliclazide was prepared using different ratios of hydroxy propyle methyl cellulose (HPMC) I
poly vinyl pyrrollidone (PVP) (3:1, 2:3, 4:1, 1:2, 2:1 and 1:4) by solvent casting (evaporating) V
method. Physicochemical parameters were characterized and dissolution studies of the E
formulated films were performed. In addition, solubility studies at various pH, thickness test, R
moisture content test and flatness test were performed. In vitro permeation studies were Y
S
done using modified Franz diffusion cells through animal skin utilizing in normal saline.
Y
Permeation studies were illustrated. Dimethyle sulfoxide (DMSO) was a good chemical
S
enhancer. The prepared films were subjected to SEM, DSC and FT-IR spectral analysis. The
T
present study resulted in optimized formulation. E
M
Keywords: Gliclazide, Transdermal patch, HMPC, PVP, Dimethyl sulfoxide, Chemical enhancer. S
S.No Formula Polymeric Drug Ratio(w/w) Plasticizer Permeation Solvent

code blend mg/cm2 Dibutyl enhancer system
phthalate DMSO
1 GLZ 1 HPMC: PVP 1 3:2 30% 1% Methanol
2 GLZ 2 HPMC : PVP 1 2:3 30% 1% Methanol
Composition of Hydroxyl Propyl Methyl Cellulose (HPMC) and PVP film Formulation .
106
FORMULATION AND IN VITRO EVALUATION OF SELF- EMULSIFYING DRUG DELIVERY D

SYSTEM OF CARBAMAZEPINE R
Swati Saxena, Chinmay Vora, Santosh Kumbhar and Chandrakant Kokare U
Department of Pharmaceutics, Sinhgad Institute of Pharmacy, Pune -411041. G
Email id: cmv277@gmail.com
D
E
Carbamazepine drug is widely used as an antiepileptic agent in therapy of
L
psychomotor seizures and trigeminal neuralgia. It is traditionally given by oral route of
I
administration but due to its poor water solubility its show slow and irregular absorption from
gastrointestinal tract. The aim of this research work was to formulate self emulsifying drug V
delivery system (SMEDDS) of Carbamazepine. The solubility of carbamazepine was E
determined in various oils, surfactant and cosurfactant. Pseudoternary diagram were used to R
determine the microemulsion area of formulation. Prepared SMEDDS were evaluated for Y
globule size, zeta potential, clarity and effect of centrifugation, assay, dilutability, refractive S
index, transmittance and stability. An optimized carbamazepine loaded formulation Y
consisting of Labrafill M 1944 CS (21.30% w/w), Chremophore RH 40 (25%), PEG 400 (25%) S
offers the advantage of good clarity systems at high oil content and thus should offer good T
solubilization of carbamazepine. It was observed that SMEDS formulation showed 85.63 % E
drug release within 25 m while conventional dosage form show only 27.95 % release. Thus M
our study confirmed that SMEDS can be used as a possible alternative to conventional oral S
formulation of carbamazepine. Results conclude that SMEDDS can be explored as a potential
drug carrier for dissolution enhancement of carbamazepine.
Pseudo – ternary phase diagrams of various ratios of oil to Composition of optimized formulations
surfactant/cosurfactant A (1:1) , B (1:2) , C(1:3) of Carbamazepine SMEDD
107
SYNTHESIS AND CHARACTERIZATION OF NOVEL AMINO ACID PRODRUG OF D

ROSIGLITAZONE R
S. Vijayaraj 1,2, G. Kalyana Chakravarthi 1, R. Shanmugam 1 U
G
1
Department of Pharmaceutical Analysis, Sree Vidyanikethan College of Pharmacy,
Sree Sainath Nagar, Tirupati, Andhra Pradesh, India D
2
Department of Pharmaceutical Sciences, NIMS University, Jaipur, Rajasthan, India.
E
Email id: vijaysurender@yahoo.co.in
L
I
Diabetes mellitus is the most common endocrine disorder. Rosiglitazone, a
V
thiazolidinedione class of antidiabetic drug widely used to treat type-II diabetes. Rosiglitazone
E
being classified under Biopharmaceutical Classification System class II drug do have poor
solubility. As solubility is an important parameter in enhancing bioavailability, majority of R&D R
focused pharma companies are targeting class II category of drugs to overcome the solubility Y
barrier. Prodrug approach is a promising effort to overcome this barrier. Hence the present S
work aims to prepare novel amino acid prodrug of rosiglitazone. The synthesized prodrugs Y
were characterized by spectral and physicochemical techniques. Chemical hydrolysis study S
was performed to ensure the stability of the prodrugs. The prepared prodrug was found to T
stable in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Reduction in Log P E
value 0.39 of prepared prodrug compared to 2.4 of drug indicates increase in hydrophilicity. M
Aqueous solubility study confirms 4.66 fold enhancement in solubility of amino acid prodrug S
of rosiglitazone compared to pure drug. Thus the synthesized amino acid prodrug of
rosiglitazone has good potential for further optimization and development.
Microscopical images of RG Amino acid prodrug
108
PHARMACOKINETICS OF ENROFLOXACIN LOADED SOLID LIPID NANOPARTICLES D

FOLLOWING ORAL ADMINISTRATION IN EMU (DROMAIUS NOVAEHOLLANDIAE) BIRDS R
P.Senthil Kumar1,, A.Arivuchelvan2, A.Jagadeeswaran2, N.Subramanian3, C.Senthil Kumar4 and
U
P.Mekala2
1
Veterinary College and Research Institute, Orathanadu-614625, Tamil Nadu, India. G
2
Veterinary College and Research Institute, Namakkal, Tamil Nadu, India. D
E
3
Department of Pharmaceutical Technology, Anna University, BIT campus, Trichy
4
Ph.D. Scholar, Dept. of Pharmaceutical Technology, Anna University, BIT campus, Trichy
Email id: p.senthilkumar@tanuvas.org.in , Phone: +91 94431 42359
L
I
The study was conducted to formulate the enrofloxacin SLNs and evaluate its V
pharmacokinetic (PK) behaviour in emus. Enrofloxacin SLNs were prepared by a hot E
homogenization coupled with ultrasonication method and characterized for further R
investigation in emus. PK of native enrofloxacin was studied after i.v. and oral bolus Y
administration at 10mg/kg in emus and it was compared with disposition kinetics of S
enrofloxacin SLNs. Enrofloxacin and its metabolite ciprofloxacin in plasma were estimated Y
using HPLC and PKs were calculated by a noncompartmental analysis. The results S
demonstrated that the particle size, polydispersity index, zeta potential, encapsulation T
efficiency and loading capacity of the SLNs were 154.72± 6.11nm, 0.42±0.11,-28.83±0.60mV, E
59.66±3.22% and 6.13±0.32%, respectively. AFM image showed spherical to circular particles M
with well defined periphery. Pharmacokinetic results showed that the t1/2β, AUC0-∞, Vdarea/F, S
MRT and F were 3.107, 1.894, 1.594, 2.993 and 1.895 times enhanced, while CL B and β were
significantly decreased by 1.958 and 3.056 times compared to the values of native
enrofloxacin. The t1/2β and MRT of the metabolite were longer than those of parent substance.
Based on the PK/PD indices, the SLNs extended the enrofloxacin concentration upto 48h.
Semlogarithmic plot of mean plasma enrofloxacin and Atomic force microscopic image of enrofloxacin
its metabolite ciprofloxacin concentration (µg/mL) vs. SLNs
time in emus (n=8) following administration of native
enrofloxacin and enrofloxacin SLNs (10mg/kg).
109
SUSTAINED OPHTHALMIC DELIVERY OF BRIMONIDINE TARTRATE FROM D

THERMO SENSITIVE IN SITU GELLING SYSTEM R
A.Waychal, P. Gijare , R. Barse, A. Tagalpallewar and C. Kokare,
Department of Pharmaceutics, Sinhgad Institute of Pharmacy, Narhe, Pune – 41.
U
Email id: alankar.waychal@gmail.com G
D
The present work reports the development of sustained release in situ gel dosage form E
for Brimonidine Tartrate that could be used for glaucoma treatment. In situ forming systems L
are free-flowing liquid solutions before administration in eye, but became gel under I
physiological conditions. In situ gel was formulated using cold method and to study the effect V
of independent variables viz. Concentrations of Poloxamer 407 and HPMC K 15 M, on various E
R
dependent variables like gelation temperature, in vitro drug release and viscosity at 37 °C., 32
Y
factorial design was employed. Further the optimized formulation was characterised for
S
sterility test, preservative efficacy, ex vivo transcorneal permeation study, texture analysis
Y
and histopathology study. Optimized in situ gel formulation shows sustained release profile S
in in vitro drug release study up to 8 h, Ex vivo transcorneal permeation through goat cornea T
using optimized in situ gel demonstrated sustained release profile as compare to marketed E
eye drop formulation. Histopathological examinations showed the better safety profile and M
non irritation potential of optimized formulation. S
. 32 factorial design studies
Name of excipients Composition of BRT in situ gel (% w/v)

F1 F2 F3 F4 F5 F6 F7 F8 F9
Brimonidine Tartrate (Drug) 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2
Poloxamer 407 16 16 16 18 18 18 20 20 20
HPMC K 15 M 0.50 0.50 0.50 0.75 0.75 0.75 1.0 1.0 1.0
BKC 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01
Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
Water 100 100 100 100 100 100 100 100 100
Composition of BRT in situ gel.
110
MODIFIED RELEASE MULTIPARTICULATE DELIVERY SYSTEM FOR TRAMADOL HCl USING D

GELUCIRE 43/01 AS RATE RETARDING MATERIAL R
Divya Priya S1, Sruthi S1, Hema R1, Rajasekar K1, Ganesan V1, Lakshamana Prabu S1,
Puratchikody A1, Shanmugarathinam A1.
U
1
Department of Pharmaceutical Technology, BIT Campus, Anna University, Tiruchirappalli, 620024, Tamilnadu. G
D
Tramadol HCl is an opioid analgesic was formulated as granules by melt granulation E
technique in the present research by using gelucire 43/01 as rate retarding material. Tramadol L
HCl is highly soluble in water and has immediate release. Gelucire 43/01 has a tendency of I
retarding the drug release profile at higher ratios. The formulated granules have been V
characterized and evaluated. The results were obtained as percentage yield (73 – 85 %) and E
drug content (69 – 98 %). FT-IR studies show that there is no interaction between drug and R
excipient. DSC analysis is used for thermal analysis of granules. The invitro release studies Y
were done for 10 hr (interval of 1 hr) by using distilled water as dissolution medium. The study
S
shows that the drug release rate gets retarded by Gelucire 43/01.
Y
S
T
E
M
S
IR spectrum of Tramadol HCl, FT-IR analysis of the Gelucire 43/01, FT-IR analysis of the Formulation (F1)
111
DEVELOPMENT AND EVALUATION OF RISPERIDONE LIPOSOMES FOR BRAIN TARGETING D

Usha Y Nayak, Mohan Singh
R
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Karnataka-
576104, India; Tel.:+91-08202922482; U
Email id: usha.nayak@manipal.edu G
D
Risperidone is an anti-psychotic drug used widely to treat schizophrenia, mixed and E
manic states associated with bipolar disorder, and irritability in people with autism, however L
it has significant first-pass metabolism with an oral bioavailability of 70% and a half-life of 3 I
hours. Also it produces extrapyramidal side effects dependent on dose. Hence in the present V
study liposomes of risperidone were prepared for nasal delivery for brain targeting to tackle
E
the problems associated with the oral delivery. Liposomes were prepared by lipid film
R
hydration followed by sonication using soyaphosphatidylcholine and cholesterol by varying
Y
lipid (2:1 to 8:1) and drug-lipid ratios (5:1 to 10:1). Formulation were optimized by Design
S
Expert 9.0 Software. The present study consists of 2 variables/factors (Lipid ratio and Lipid
drug ratio) and 3 levels for each of them (2:1, 4:1, 8:1 and 5:1, 7.5:1, 10:1). Liposomes were Y
evaluated for Mean vesicle size, poly dispersibility index (PDI), Zeta potential, Encapsulation S
Efficiency and In vitro release studies. Morphological of the optimized liposomes were studied T
by Transmission Electron Microscopy (TEM). The average particle size and PDI of optimized E
risperidone liposomes was found to be 91.30 nm (Fig. 1) and 0.255 respectively. The zeta M
potential was - 53.9 indicating stable formulation. It was found that lipid/drug ratio of 10:1 S
has given greater % drug entrapment (49.6 %) compared to others. % EE was increasing with
increasing amount of lipid to drug molar ratio. Also the same formulation showed maximum
drug release 56.98 % at the end of 4 h. TEM images confirmed spherical shape of the
liposomes and uniform size distribution.
Average particle size and PDI of optimized Risperidone liposomes
112
NANOPARTICLE-APTAMER BIOCONJUGATES: A FOCUS ON TARGETED CANCER THERAPY D

P.Sushma
R
M-Pharmacy (Pharmaceutical Analysis & Quality Assurance)
Email id: sushma.pallekona303@gmail.com U
G
Among the major challenges in Cancer Therapy is development of agents that can be D
used for early diagnosis and effective therapy. Nanoparticle platforms offer a variety of E
potentially efficient solutions for development of targeted agents that can be exploited for L
cancer diagnosis and treatment. A wide range of nanomedicines such as polymeric micelles, I
liposomes, and polymeric nanoparticles have been extensively explored for targeted delivery V
of anti-cancer agents, for they can accumulate in the solid tumor site via leaky tumor vascular E
structures, thereby selectively delivering therapeutic payloads into the desired tumor tissue. R
These novel strategies for targeted cancer therapy are in great demand for effective cancer Y
treatment. One of the targeting approach i.e., Active targeting is achieved by conjugating
S
nanomaterials with targeting ligands that bind to overexpressed antigens or receptors on the
Y
target cells. This specific binding to targeted cells leads to an increased accumulation of
S
nanomaterials on target cells while minimizing harmful toxicity to non-target cells. Aptamers
are novel oligonucleotides with flexible three-dimensional configurations that recognize and T
bind to their cognate targets, including tumor surface receptors, in a high-affinity and highly E
specific manner. These exhibit advantages such as low cost, low immunogenicity and toxicity, M
a smaller size to enable solid tumor penetration and high affinity to bind with the target, all S
of which make aptamers as target ligands for specific cancer cell recognition and targeted
cancer therapy. Recently, the development of aptamer-conjugated nanomaterials has offered
new therapeutic opportunities for cancer treatment with better efficacy and lower toxicity.
Aptamer-conjugated nanomaterials are being incorporated into emerging technologies with
significant improvement may provide a more efficient and minimize systemic cytotoxicity, a
approach to meet the growing demands for novel strategies in the fight against cancer.
Targeted Therapy By Aptamers
113
NANO-ENCAPSULATED GINSENOSIDES: A NOVEL APPROACH TOWARDS TARGETING SOD1 D

AND TARDBP IN AMYOTROPHIC LATERAL SCLEROSIS (ALS) R
Kavya TK, Rachana Srinivasa and Savithri Bhat
Department of Biotechnology, BMS College of Engineering, Bull temple road, Bangalore 560019, India.
U
E mail: savithri.bhat@gmail.com, 91-9535067633 G
D
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that E
attacks the motor neurons of the brain and spinal cord of healthy adults. The disease L
progresses rapidly and is fatal, leaving patients paralyzed and unable to breathe. The I
pathological determinants of disease progression remain poorly understood. Currently, there V
is no known cure or effective treatment available for ALS. But with the advances in E
technology, there has been enormous increase in the volume of the genetic data produced R
and thus stored for analysis and interpretation. The present study is focused on one such Y
usage of the data extracted from ALS Online Database (http://alsod.iop.kcl.ac.uk). The study
S
is based on the insilico approach for selecting appropriate targets and screening suitable
Y
ginsenoside, an herbal drug molecules against targets of ALS. The patient’s data and their
S
respective targets were obtained from ALSoD. The selection of the targets, SOD1 and TARDBP
is based on the reports and the frequency as well as the types of mutations occurring in the T
population under study. The structure of the targets were modeled using SWISSMODEL E
.About 50 ginsenoside and their derivative obtained from Pubchem compound database were M
screened and docked against the targets . Further, it is proposed that the nano-encapsulated S
ginsenoside with specific receptors for the target cells would increase the efficacy of
ginsenoside by increasing its bioavailability.
Ginsenoside Rb docked to SOD1 target
114
DEVELOPMENT AND SUSTAINABLE RELEASE EVALUATION OF ENROFLOXACIN SOLID LIPID D

NANOPARTILCES R
P.Senthil Kumar1,, A.Arivuchelvan2, A.Jagadeeswaran2, N.Subramanian3, C.Senthil Kumar4 and
U
P.Mekala2
1
Veterinary College and Research Institute, Orathanadu-614625, Tamil Nadu, India. 2Veterinary College and G
Research Institute, Namakkal, Tamil Nadu, India. 3 Department of Pharmaceutical Technology, Anna University, D
BIT campus, Trichy 4Ph.D. Scholar, Dept. of Pharmaceutical Technology, Anna University, BIT campus, Trichy E
Email id: p.senthilkumar@tanuvas.org.in , Phone: +91 94431 42359
L
I
The study was conducted to formulate the enrofloxacin solid lipid nanoparticles (SLNs) V
with sustained release profile and improved pharmacological activity. The enrofloxacin SLNs
E
were prepared using tripalmitin as lipid carrier, tween 80 and span 80 as surfactants and poly
R
vinyl alcohol (PVA) as a stabilizer by a hot homogenization coupled with ultrasonication
Y
method. The formulation were characterized for particle size, polydispersity index, zeta
S
potential (using dynamic light scattering), shape (using atomic force microscopy), drug
encapsulation efficiency (using by dialysis and ultracentrifugation methods), and in vitro drug Y
release (using by dialysis). The prepared SLNs were analyzed by FT-IR spectroscopy to confirm S
the cross-linking reaction between drug, lipid and surfactants. The results demonstrated that T
encapsulation efficiency, loading capacity, diameter, polydispersity index and zeta potential E
of the nanoparticles were 59.66±3.22%, 6.13±0.32%, 149.4±2.50 nm, 0.314±0.004, and - M
24.90±1.00 mV. The prepared nanoparticle was spherical in shape with smooth surface. In S
vitro release study in phosphate buffer saline (pH 6.7) showed an initial burst release and
followed by a slow and sustained drug release. FT-IR study showed no interaction between
the drug, polymer and excipients in the present study. From the results, it can be concluded
that the enrofloxacin-loaded tripalmitin SLN proved promising formulation for sustained
release.
Physico-chemical characteristics of enrofloxacin loaded SLN (mean±SD, n=3)
Particle Size (nm) PDI Zeta Potential Encapsulation Loading Capacity

(mV) Efficiency (%) (%)
149.4±2.50 0.314± 0.004 -24.90±1.00 59.66±3.22 6.13±0.32
Atomic force microscopic 3D image of In vitro release of native enrofloxacin and

enrofloxacin SLN enrofloxacin SLNs (mean± SD, n=3).
115
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International Conference on Innovations and Future Research Dimensions on Nanobio Pharmaceutical Technology

DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY

Deeply inoculate the treasure of pharmacy education and research into

Provide a dynamic educational experience, create outreach

Advocate the responsibility to contribute for the improvement in public

(Dr.S.Latha & Dr.P.Selvamani)

CHAIR Dr.T.Senthil Kumar

S.MONISHA M.Tech., Final Year

T.SUPASSRI M.Tech., Final Year

INTERNATIONAL ADVISORY BOARD MEMBERS

SILVIO DUTZ Technische Universität Ilmenau, Germany

TANAJI T. TALELE St. John’s University, USA

JAGAT KANWAR Deakin University, Australia

MANIKAM SIVAKUMAR The Nottingham University, Malaysia

R.THIRUMURUGAN International Medical University, Malaysia

R. RAJAN International Medical University, Malaysia

L. MANIKANDAN Forma Therapeutics, Singapore

RENGANATHAN ARUN University of Zurich, Switzerland

ASHOK KUMAR ASIA Metropolitan University, Malaysia.

RAVI PALANIAPPAN Mercer University, Atlanta, USA

JITKANG LIM Universiti Sains Malaysia, Malaysia

KARINA POMBO GARCIA Helmholtz Zentrum Dresden Rossendorf, Germany

SHEEBA DAVID International Medical University, Malaysia.

DEEPAK BALAJI Thimiri Consulting Group, France

NIRMESH JAIN University of Sydney, Australia

VASUDEVAN MANI Universiti Teknologi MARA, Malaysia

R. MANAVALAN Annamalai University, Tamil Nadu, India

RAJIV DAHIYA Global College of Pharmacy, UP, India

T.K. PAL Bioequivalence Study Centre, West Bengal, India

DHIRENDER BAGADHUR IIT, Mumbai

P.JAISANKAR Indian Institute of Chemical Biology, West Bengal,

P. GAUTAM Anna University, Tamil Nadu, India

M. KRISHNAN Bharathidasan University, Tamil Nadu, India

P. RAJAGURU Anna University, Tamil Nadu, India

G.P. MOHANTA Annamalai University, Tamil Nadu, India

S. KAVIMANI Mother Teresa Post Graduate and Research

M.V. RAO Bharathidasan University, Tamil Nadu, India

T.N.K. SURIYA PRAKASH Al-Shifa College of Pharmacy, Kerala, India

P. GOPINATH IIT, Roorkee

RANU DUTTA University of Allahabad, Uttar Pradesh, India

L.K. GHOSH Jadavpur University, West Bengal, India

B.S. LAKSHMI Anna University, Tamil Nadu, India

G. MATHAN Bharathidasan University, Tamil Nadu, India

R. SIVAKUMAR Grace College of Pharmacy, Kerala, India

G. MARIAPPAN Kamla Nehru Institute of Management and

R.S. JEYA PRAKASH Manipal College of Pharmaceutical Sciences,

M.B. VISWANATHAN Bharathidasan University, Tamil Nadu, India

T.K. RAVI Sri Ramakrishna Institute of Paramedical Sciences,

V. RAJESHKANNAN Bharathidasan University, Tamil Nadu, India

K. ASOKKUMAR Sri Ramakrishna Institute of Paramedical Sciences,

CODE TITLE / AUTHOR PAGE NO

NM-OP-01 Synergistic antibacterial efficacy of two drugs in addition with 1

NM-OP-02 Green synthesis and characterization of herbal mediated silver nano 2

NM-OP-03 Bio-toxicity of silver nanoparticles against multidrug resistant 3

NM-OP-04 Biosynthesis of silver nanoparticles using cloves of Allium sativum and 4

NM-OP-05 Physiochemical properties of nanocomposite film made of chitosan 5

NM-OP-06 Larvicidal and antimicrobial potential of silver nanoparticles from a 6

NM-OP-07 Formulation and evaluation of mouth dissolving film leflunomide for 7

NM-OP-08 Antimicrobial, antioxidant and angiogenic potential of silver 8

NM-OP-09 Antioxidant and hemolytic property of silver nanoparticles synthesized 9

NM-OP-10 Tinidazole loaded nanoparticles for treatment of periodontitis: 10

NM-OP-11 Development of semisolid drug carriers for topical application based on 11

NM-OP-12 Improvement of physicochemical properties and antiangiogenic 12