Documente Academic
Documente Profesional
Documente Cultură
Ms. Keith
AP Biology
January 7, 2011
Article Critique
Throughout the science community, one may see that there are thousands of studies and
experiments running around the world. Scientists have the opportunity to study whatever they
desire. In this study, one may view a study on Totipotent stem-cells. The article, “Proliferation
of Totipotent Hematopoietic stem-cells in vitro reconstituting ability,” one can look deeper into
the Totipotent stem-cell research. This article was retrieved from Proc. Natl. Acad. Sci. USA
Reading through the article, one can see many different sides and ideas that may come
with a deeper look. One can tell that the study type was a longitudinal study. This means the
study was a long term study, one which lasted longer than two years. This study contained many
methods, also. The methods included; they received the virus through scraping it out of the dish
that was then washed in fetal calf, they then had aliquots over laid on the established B6C3F
marrow, removed half of the nonadherent cells and restored it with fresh LTC medium. They
also did things like they injected irradiated to recipients, used Hematopoietic tissue analysis, they
obtained elution of nonadherent cells, they used the southern blot analysis, and so on. With the
opportunity of this experiment, there were many results that came with the conclusion of the
experiment. Some of the results included were dominate cones allowing comparison of the
retrovirus, the recovery of long term repopulating cells, quantitation of CRU in LTC, evidence of
Totipotent stem-cell amplification and serial studies of repopulation stem-cell clones during
LTC. The conclusion of the lab was the operation of human LTC has similar mechanics to that
of stem-cell proliferation and decline. This allows manipulation to change conditions that allow
Throughout the completion of this lab, one could see the different sides of the article.
One thing someone may have been able to see was the strengths of the article. Some of the
strengths included were they used a recombinant Tkneo19 virus, they displayed their results very
clearly, and they repeated the experiment many times. Another thing one may be able to see was
they used 3 different kinds of animal products which, could potentially skew the results in they
lab which could show an incompletion in the study. The article was very relevant and it allowed
one to see how this study can help with gene therapy proto call in the future. This could allow
the world to try and fix viruses and other things that could cause detrimental damage in the
environment.