Last Update: diciembre 18, 2019

General

1. Description

1.1 Flowsheet

S-104

S-101 S-105
P-6 / SFR-102 S-103
S-10
SIEMBRA 50 L
S-113

S-116
P-2 / V-101 S-102 S-114 S-107
P-16 / SFR-103
TANQUE DE MEZCLADO
S-112 SIEMBRA 500 L
P-3 / ST-101
Heat Sterilization
P-1 / FSP-101 S-117

Flow Splitting S-110

P-7 / AF-101
S-106 S-109 Air Filtration

S-124
P-5 / G-101
Gas Compression
S-120 S-119
S-125P-12 / V-103

Flash P-11 / MX-101

S-121 P-9 / UF
Mixing S-123
Ultrafiltr
P-14 / V-104
S-126 Storage
S-122
P-10 / UF-102
Ultrafiltration
DS-102 DS-101

P-13 / FL-101
Filling

1.2 Filename (.spd) C:\Users\usuario\Desktop\SIMULACION Anticuerpos Monoclonales\hibridomas mejora 1.spf

2. Economics
2.1 Total Investment 17607953 $
2.2 Total Revenues 0 $/yr
2.3 Operating Cost 49601087 $/yr
2.4 Unit Production Ref. Rate 0.00 kg MP/yr
2.5 Unit Production Cost
incl. depreciation 0.00 $/kg MP
excl. depreciation 0.00 $/kg MP

3. Project Indices

3.1 Gross Margin -1.00 %

3.2 Return On Investment -274.85 %
3.3 Payback Time N/A
3.4 IRR Before Taxes 0.00 %
3.5 IRR After Taxes 0.00 %
3.6 NPV at (7.00 %) 0$

4. Size

4.1 Throughput 0.00 kg MP/hr

4.2 Annual Throughput 0.00 kg MP/yr
MP = Undefined
 S-108

S-107

S-115

S-110

P-4 / AFR-101
S-111
Air Lift Fermentation

S-118 P-8 / V-102

P-9 / UF-101
Storage
Ultrafiltration
 Last Update: diciembre 18, 2019
Materials

1. Tables

1.1 Material Consumption

Material kg/yr kg/h kg/kg MP
Air 2,838,811.90 358.44 N/A
aminopterina 1,980.00 0.25 N/A
hipoxantina 19,800.00 2.50 N/A
timidina 6,336.00 0.80 N/A
Water 367,884.00 46.45 N/A
TOTAL 3,234,811.90 408.44 N/A

1.2 Material Cost

Unit Cost Annual Annual Cost

Bulk Material %
($) Amount ($)

Air 0.000 2,838,812 kg 0 0.00

aminopterina 10,000.000 1,980 kg 19,800,000 49.80
hipoxantina 1,000.000 19,800 kg 19,800,000 49.80
timidina 10.000 6,336 kg 63,360 0.16
Water 0.250 367,884 kg 91,971 0.23

Unit Cost Annual Amount

Discrete Material Annual Cost($) %
($/Entity) (Entities)

hibridomas 0.000 630,270 0 0.00

TOTAL - - - 39,755,331 100.00

1.3 Overall Balance

(kg/h)
COMPONENT IN OUT OUT-IN
aminopterina 0.25 0.02 - 0.23
Carb. Dioxide 0.00 0.18 0.18
hibridomas 0.00 0.77 0.77
hipoxantina 2.50 1.93 - 0.57
Nitrogen 274.96 274.96 0.00
Oxygen 83.47 83.47 0.00
timidina 0.80 0.46 - 0.34
vial 0.79 0.79 0.00
Water 46.45 46.63 0.18
TOTAL 409.23 409.23 0.00
 Last Update: diciembre 18, 2019
Utilities

1. Tables

1.1 Heat Transfer Agent Demand

1.1a Total Heat Transfer Agent Demand
Heat Transfer Agent kg/yr kg/h kg/kg MP
Chilled Water 38,345,106.26 4,841.55 N/A
Cooling Water 34,357,880.63 4,338.12 N/A
Steam 533,629.05 67.38 N/A
Steam (High P) 0.00 0.00 N/A

1.1b Heat Transfer Agent Demand (Section Breakdown)

Chilled Water kg/yr kg/h kg/kg MP
Main Section 38,345,106.26 4,841.55 N/A
TOTAL 38,345,106.26 4,841.55 N/A

Cooling Water kg/yr kg/h kg/kg MP

Main Section 34,357,880.63 4,338.12 N/A
TOTAL 34,357,880.63 4,338.12 N/A

Steam kg/yr kg/h kg/kg MP

Main Section 533,629.05 67.38 N/A
TOTAL 533,629.05 67.38 N/A

Steam (High P) kg/yr kg/h kg/kg MP

TOTAL 0.00 0.00 N/A

1.1c Heat Transfer Agent Demand (Procedure Breakdown)

Chilled Water kg/yr kg/h kg/kg MP
Main Section
SFR-102 : P-6 328,734.75 41.51 N/A
SFR-103 : P-16 3,287,898.37 415.14 N/A
AFR-101 : P-4 34,728,473.14 4,384.91 N/A
Section Total 38,345,106.26 4,841.55 N/A
TOTAL 38,345,106.26 4,841.55 N/A

Cooling Water kg/yr kg/h kg/kg MP

Main Section
ST-101 : P-3 1,582,095.22 199.76 N/A
G-101 : P-5 32,775,785.41 4,138.36 N/A
Section Total 34,357,880.63 4,338.12 N/A
TOTAL 34,357,880.63 4,338.12 N/A

Steam kg/yr kg/h kg/kg MP

Main Section
ST-101 : P-3 10,780.72 1.36 N/A
V-103 : P-12 522,848.33 66.02 N/A
Section Total 533,629.05 67.38 N/A
TOTAL 533,629.05 67.38 N/A
Steam (High P) kg/yr kg/h kg/kg MP
TOTAL 0.00 0.00 N/A

1.2 Power Demand

1.2a Total Power Demand
Power Type kW-h/yr kW-h/h kW-h/kg MP
Std Power 535,516.33 67.62 N/A
TOTAL 535,516.33 67.62 N/A

1.2b Power Demand (Section Breakdown)

Std Power kW-h/yr kW-h/h kW-h/kg MP
Main Section 535,516.33 67.62 N/A
TOTAL 535,516.33 67.62 N/A

1.2c Power Demand (Procedure Breakdown)

Std Power kW-h/yr kW-h/h kW-h/kg MP
Main Section
SFR-102 : P-6 2,013.85 0.25 N/A
SFR-103 : P-16 20,132.68 2.54 N/A
G-101 : P-5 201,149.26 25.40 N/A
UF-101 : P-9 3,187.30 0.40 N/A
AFR-101 : P-4 201,326.03 25.42 N/A
UF-102 : P-10 603.95 0.08 N/A
Unlisted Equipment 26,775.82 3.38 N/A
General Load 80,327.45 10.14 N/A
Section Total 535,516.33 67.62 N/A
TOTAL 535,516.33 67.62 N/A

2. Charts
 Last Update: diciembre 18, 2019
Equipment

1. Charts
 Last Update: diciembre 18, 2019
Labor

1. Tables

1.1 Labor Demand

1.1a Total Labor Demand
Heat Transfer Agent labor-hrs/yr labor-hrs/h labor-hrs/kg MP
Operator 106,844.57 13.49 N/A

1.1b Labor Demand (Section Breakdown)

Operator labor-hrs/yr labor-hrs/h labor-hrs/kg MP
Main Section 106,844.57 13.49 N/A
TOTAL 106,844.57 13.49 N/A

1.1c Labor Demand (Procedure Breakdown)

Operator labor-hrs/yr labor-hrs/h labor-hrs/kg MP
Main Section
V-101 : P-2 11,314.29 1.43 N/A
ST-101 : P-3 5,657.14 0.71 N/A
SFR-102 : P-6 11,314.29 1.43 N/A
SFR-103 : P-16 11,314.29 1.43 N/A
G-101 : P-5 565.71 0.07 N/A
AF-101 : P-7 113.14 0.01 N/A
UF-101 : P-9 13,200.00 1.67 N/A
V-102 : P-8 11,314.29 1.43 N/A
V-103 : P-12 5,657.14 0.71 N/A
FL-101 : P-13 565.71 0.07 N/A
V-104 : P-14 11,314.29 1.43 N/A
AFR-101 : P-4 11,314.29 1.43 N/A
UF-102 : P-10 13,200.00 1.67 N/A
Section Total 106,844.57 13.49 N/A
TOTAL 106,844.57 13.49 N/A

2. Charts