Answers

1.

I 1 2

II 1 2 3 4

Mom Jones Dad Jones Mom Smith Dad Smith

III 1 2 3 4 5 6 7 8

Twin Jones Smith Smith

Smith Son Smith Smith Smith
Son Son
Daughter Son Son

2.

ug/ul of
Sample A280 A260 A250 A230 A260/A280 A260/A250 A230/A260 stock

0.189 0.350 0.313 0.151

1 1.8518519 1.1182109 0.43142857 17.5

0.054 0.095 0.084 0.041

2 1.7592593 1.1309524 0.43157895 4.75

0.159 0.142
3 0.089 0.072 1.7865169 1.1197183 0.45283019 7.95

0.111 0.201 0.180 0.092

4 1.8108108 1.1166667 0.45771144 10.05

0.237 0.212 0.108

5 0.130 1.8230769 1.1179245 0.4556962 11.85

0.128 0.231 0.206 0.102

6 1.8046875 1.1213592 0.44155844 11.55

7 0.032 0.048 0.043 0.029 1.5 1.1162791 0.60416667 2.4

 0.057 0.101 0.090 0.046
8 1.7719298 1.1222222 0.45544554 5.05

0.141 0.256 0.230 0.117

9 1.8156028 1.1130435 0.45703125 12.8

0.122 0.220 0.196 0.098

10 1.8032787 1.122449 0.44545455 11

0.086 0.151 0.135 0.072

11 1.755814 1.1185185 0.47682119 7.55

0.270 0.242 0.134

12 0.149 1.8120805 1.1157025 0.4962963 13.5

The DNA ration at 260/280=1.8-2.0 shows the purity of DNA samples which is required for
restriction digestion experiment. The sample No 1, 2, 7, 8 and 11 showed less than1.8 OD ratio
which indicates the DNA is not pure. Therefore, these samples revealed proteins contamination
that cannot generate proper restriction digestion pattern as DNA itself when use. Ergo, these
DNA samples cannot be used for restriction digestion experiment.

3.
The volume will be : 10 x 25 x 0.5 = 125 cc
Therefore, 125 ml of the 1.25% agarose gel is to be used.
Total amount of TAE used is 125 ml.
Total amount of agar used is 1.56 gram because you need 1.25 grams of agar per 100 ml
of TAE. Therefore, by ratio and proportion, (1.25 g x 125 mL)/100 mL.

4. a. No
b. There must be an error during the electrophoresis. It seems that in the photo, the
visualization was improper. It seems that the material use in visualizing the samples were
too high, whether it was a dye or a radioactive probe.
c. To resolve this, the controlled and correct process should be followed to create a
proper visualization. The exact amount to use for the process should not be too high or
too low to create a visual that is accurate and is precise.
5. A. If child 3,4, and 6 have the disease, this means that the marker for the disease allele is
a single band. Therefore, other who have extra bands do not have the disease. Meaning, it
could be a recessive disorder.
 B. The carriers will be the people with this single band plus another band which is 1, 2, 8,
9, 10, and 11.

C. It must be an autosomal recessive disorder.

6. Smith's family gel run shows two band derived from Smith's mother DNA digestion and gel
run while Lane 8 shows two band derived from Smith's father DNA digestion and gel run. The
two band may indicate the heterozygous state for the locus on which digestion is done. All the
children of smith have two bands, except Smith's son 5, and the size of these band is either
similar to the size of one or both the bands of Smith's mother or father. Smith's son 5 do not
show any band because may be the chromosome on which the digestion was done is lost in that
child.
Jone's family gel run shows two band derived from Jone's mother DNA digestion and gel run
while Lane 12 shows one band derived from Jone's father DNA digestion and gel run. One band
may indicate that the Jone's father is homozygous for the locus on which the digestion is done.
Both the children of Jone shows two bands and the size of these band is either similar to the size
of one or both the bands of Jone's mother or father.
7. a. s
1 2 3 4 5 6 7 8 9 10 11 12

b. Smith daughter is not a carrier. To be a carrier, she should have the band and an additional
band.
c. This is if Dad Jones is affected too.
1 2 3 4 5 6 7 8 9 10 11 12