Rev005_2011-11-30

Service Note: Changing the Flow Cuvette (04-100-1002)

CyFlow® Counter II (CY-S-3022), CyFlow® SL_3

All procedures described in this service note should only be performed by authorized and trained technicians.

Warning: All systems used to analyze biohazardous and carcinogenic materials (infectious materials, dyes),
must be decontaminated before exposure to persons who are unlikely to apply proper contaminated
materials handling techniques. In case of accidental contact, wash your skin thoroughly with soap
and disinfectant. Always wear protective gloves when working with the BioSafety Sample Port, Flow
Cuvette or tubings.

Note: Make sure that the instrument is switched off and the line voltage is disconnected.
1. Remove the sheath fluid and sample tube from the Flow Cuvette.
2. Loosen the screw for the xy-stage. (Fig.1)
3. Remove the xy-stage.
4. Loosen the screw for the Flow Cuvette at the xy-stage. (Fig.1)
5. Remove the Flow Cuvette from xy-stage.
6. Remove waste tube from Flow Cuvette.
Note: Support the Flow Cuvette at upper end when removing the waste tube. (Fig.2)
7. Connect waste tube to the new Flow Cuvette.
Note: Support the Flow Cuvette at upper end when connecting the waste tube. (Fig.2)
8. Place the new Flow Cuvette into the xy-stage.
9. Hand-screw the screw for the Flow Cuvette at the xy-stage. (Fig.1)
10. Connect the sheath fluid and sample tube to the Flow Cuvette.
11. Put the xy-stage back in place and hand-tight the screw for xy-stage. (Fig.1)
Note: Make sure that there is no dust or fingerprints on the surface of the Flow Cuvette.
Only use diluted dishwashing detergent for cleaning the Flow Cuvette surface and additionally with
distilled water if necessary.
Only use Cleaning Solution (Order No. 04-4009) or Decontamination Solution (Order No. 04-4010)
for cleaning the Flow Cuvette inside.
Do not use ultrasonic devices, hot water or organic solvents (e.g. alcohol, acetone, benzene) to
clean the Flow Cuvette.
12. Run Calibration Beads (Oder No. 05-4018) and perform an optical alignment if necessary.

Warning: Risk of breaking Flow Cuvette at objective if objective is turned too far in direction of the Flow Cuvette.

Screw for Screw for

Flow Cuvette xy-stage

Fig.1 Flow Cuvette in xy-stage Fig.2 Supporting the Flow Cuvette

Partec GmbH  Am Flugplatz 13  02828 Görlitz  Germany
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