Reagent Operation Guide HumaCount 5D

Please read the User Manual before using the Quick Guide

1 Preparation
WARNING

• Reagents can be irritating to the eyes, skin and mucosa. Wear proper personal protective equipment (e.g.
gloves, lab uniforms, etc.) and follow laboratory safety procedures when handling them in the laboratory.
• If the reagent accidentally comes in contact with your skin, wash it off immediately with plenty of water
and see a doctor if necessary. Do the same if you accidentally get any of the reagents in your eyes.

NOTE After long-distance transportation, the reagent must be allowed to settle for more than one day before
use.

2 Loading Reagents
Once an alert about insufficient reagent is displayed, you need to load and replace the respective reagent.
Alternatively, when the reagent pack is low or close to expiration, you can choose to change it immediately
by clicking on the reagent symbol located on the left side of the screen to access the reagent management
setting interface.
Here we will explain the process of loading new reagents using the example of the HC5D Diluent. The same
steps need to be performed for HC5D CBC Lyse and HC5D Diff Lyse.

1. When the „HC5D-Diluent not enough.“ alert is displayed on

the left side of the screen, click the message. A dialogue box as
shown in Figure 1 will pop up.

2. Connect a new container of HC5D-Diluent to

the analyzer.
3. Take out the RF card from the inside of the
new HC5D-Diluent container. See Figure 2 as
a reference.
4. Select the error name in the pop up dialogue
Figure 1 box and click “Remove Error”. A dialogue box
as shown in Figure 3 will pop up.
5. Place the RF card in front of the RF card read-
er symbol on the analyzer:

Figure 3

The beeping of the card reader and a pop up

dialogue box as shown in Figure 4 indicate
successful upload of reagent credits.
Figure 2
Figure 4

6. Click OK to complete the replacement of the reagent as shown in Figure 4. The error will be removed au-
tomatically.
7. After replacement of the reagent, run a background test to verify that the results meet the requirements.

WARNING

Dispose of reagents, waste, samples, consumables, etc. according to local legislations and regulations.

16450/5-3

HUMAN Gesellschaft für Biochemica und Diagnostica mbH

Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
Tel. +49 6122-9988-0 · Fax +49 6122-9988-100 · e-mail: human@human.de · www.human.de
 Reagent Operation Guide HumaCount 5D
Please read the User Manual before using the Quick Guide

NOTE • The RF card is intended for single use only. Mark the RF card as used.
• If the RF card verification fails, please follow the system prompts and use a valid RF card.
• For the replacement of Lyses, cross-cut the seal of the bottles. Please be careful to prevent sealing
fragments from falling into the lyse bottles.

3 Reagent Management
• Once the new reagent is connected to the analyzer, you can set the reagent configurations in the Reagent
Management interface as shown in Figure 5.

After completing the reagent settings, you should perform the re-
agent replacement operations. You can select to replace one type
of reagent at a time or all Reagents at once.

The procedure will be performed as follows:

• Select a type of reagent to be replaced, and click Replace; alterna-
Figure 5 tively click Replace All to replace all reagents.
• After the replacement is completed, a message box will pop up
on the screen. See Figure 6.

• Click OK to close the message box.

Figure 6

NOTE After reagent replacement, run a background check to verify that the results meet the
requirements.

4 Troubleshooting
Error Description Solution
“Reagent name” not enough. Refer to paragraph 2: Loading Reagents.
e.g. „HC5D-Diluent not enough“

“Reagent name” expired
e.g. „HC5D-Diluent expired“
1. Check if the reagent is expired. If so, replace it with a new con-
tainer.
2. Click the error information on the left side of the screen. Click
Remove Error in the pop-up dialogue box.
3. If the error still exists, contact our customer service department.

“Reagent name” running out
(or air bubbles in inlet tubing).
e.g. “HC5D-Diluent running out.
(or air bubbles in inlet tubing).“
1. Check whether the reagent is running out. If so, please install a
new container.
2. Click the error information on the left side of the screen. Click
Remove Error in the pop up dialogue box.
3. If the error still exists, contact our customer service department

NOTE After reagent replacement, run a background check to verify that the results meet the
requirements.

16450/5-3

HUMAN Gesellschaft für Biochemica und Diagnostica mbH

Max-Planck-Ring 21 · 65205 Wiesbaden · Germany Rev. 01/2019-03
Tel. +49 6122-9988-0 · Fax +49 6122-9988-100 · e-mail: human@human.de · www.human.de