Name:_____________________ http://www.instructionaltechsupport.com/?

p=479 Date:________

What is gene splicing?

IT IS VERY IMPORTANT THAT YOU READ ALL THE INSTRUCTIONS AND FOLLOW THE
DIRECTIONS.
Gene splicing is cutting a ____________ from ___ organism and ____________ it into the ___ of
another so that a ____________________ can be transferred from __ plant or animal to another

____________ can make ____________ ____________ for diabetics to use if the insulin gene is cut
from a human chromosome and pasted into a bacterium.

Click Continue

What are you going to do in this activity?

Click Continue

Define “Restriction Enzymes”

What happened when you used the EcoRI restriction enzyme?

Which restriction enzyme cut the DNA molecule where you wanted it?

Notice the difference in DNA shapes? The top DNA molecule is linear and double stranded. The new
molecule is still DNA, but it is called a plasmid. What shape is the plasmid

Which enzyme do you use to cut the plasmid?

After the new gene is placed into the plasmid, what do you do next?

Which enzyme do you use to seal the plasmid with the new gene sequence?

DNA FINGERPRINTING: A KILLERʼS TRAIL

DNA. It's what makes you unique. It's the stuff that tells each and every one of your body's
____________cells what it's supposed____________ and what it's supposed ____________. And
although your DNA is different from that of every other person in the world—unless you have an
____________ ____________—it's the same in every cell that makes up your body.
Name:_____________________ http://www.instructionaltechsupport.com/?p=479 Date:________

But what exactly is a DNA fingerprint? Well, it certainly isn't an inky impression of a DNA strand.
Compared to unimaginably small DNA, a fingerprint is ____________.

Click on “PART 1: IT TAKES A LICKIN

Read everything carefully. Where is the DNA coming from?

Click on “PART 2: DNA FINGERPRINT AT THE NOVA LAB

Read the instructions identifying the necessary steps to create a DNA fingerprint. After each step a
pop-up window will tell you what is happening. From those pop-up windows answer the following
questions.
What did the restriction enzyme do to the DNA?

After you add the agarose gel, what does it say agarose gel is and what does it act to do?

What is electrophoresis?

What electrical charge does the DNA have:_____________ and which end of the tray does it move
towards:__________

Where do you transfer the DNA after running the gel electrophoresis

Why do you use x-ray film?

Who is the “Culprit” and how did you know she was the culprit?

Now that you are finished consider some other useful ways that DNA Fingerprinting can be applied.
List at least 2 other reasons scientists or doctors do DNA fingerprinting.

What are your thoughts about genetic counseling and possible genetic therapy to have a future
“designer child”. If you aren’t sure what the question is asking search online to learn more about how
the future might allow you to pick and choose traits for your child. If it was available would you do
that, and if so, which traits would you choose?