Cleaning the flow lines ‘Once a system which includes the CDD-10Avp/10Asp has been completely assembled, flush the flow lines thoroughly before connecting the column, Similarly, clean the flow lines of systems which have been inactive for over a month to remove possi- ble contamination. If the column and the suppressor cartridges have already been removed, skip to step 3 below. 1, Remove the column. Connect the column inlet tubing directly to the column outlet tubing using the PEEK male connector or coupling C. 2. CDD-10Asp only: Slide the suppressor/detection unit cover up and pull it towards you to remove it. Remove the suppressor cartridges from the suppressor/detection unit and connect the inlet to the outlet tubing using the special dummy coupling (dummy coupling, P/N: 228- 36573). (Use the dummy coupling that was shipped inthe unit inthe place of the suppressor asap cartridge.) ne 3 3. Tum ON the pump and deliver ethanol at 1.0 mL/min for 10 minutes for cleaning. All if cleaning solvents described in this section are delivered at flow rate of 1.0 mL/min 4. Flush with deionized water for 5 minutes, then deliver IN nitric acid solution for 10 min- 3 utes. z 5. Flush with deionized water for S minutes, then 0.1% ethylendiaminetetracetic acid disodi- 8 um salt (EDTA-2Na) solution for 30 minutes. = 6. Finally, deliver deionized water for 30 minutes. 2 a z cover my (—= a —<——} SS —= Fig. 5.37 5-31 CDD-10Avp/10Asp ST