Documente Academic
Documente Profesional
Documente Cultură
PCASTRD- DOST
Funding Agency
Genetic engineering
• Identify genes responsible for
desired traits
• Understanding genetic control of
ripening
Banana ripening
MADS-Box Genes
o Transcription factors
o Roles in floral development
are well established
o Modular structure for DNA
binding
Specific Objectives
Promoter region
MH5R (5’ GCAGAACTCGTACAGCTTCC 3’).
Gene Sequences of MaMADS2 in
Lakatan and Saba are now available
In Lakatan, the MaMADS 2 gene was
found to have three isoforms; Types I, II
and III
Lakatan and Saba have the same
translated region
The isoforms have similar nucleotide
sequences with insertions and deletions on
some sites
Very notable that the isoforms have clear
differences in the 3’ UTR regions
Three isoforms of Lakatan MaMADS2 gene vs Cavendish MaMADS2
Type_I
MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIVFSN 50
RGKLYEFCSSSSMLKTLERYQKCNYGAPETNIISREIQTSQQEYLKLKAR 100
VEALQRSQRNLLGEDLGPLSIKELEQLERQLDASLRQIRSTRTQCMLDQL 150
ADLQRR-EQMLCEANKALKIRMDEGNQANQQQLWDP-NAHAVAYCRHQPQ 198
Type_II
MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSN 50
RGKLFEFCSSSSMLKTLERYQKCNYGAPETNVMSRETQNSQQEYLKLKAR 100
VEALQRSQRNLLGEDLGPLNIKELEQLERQLDASLRQIRSTRTQYMLDQL 150
TDLQRR-EQMLCEANKALKRRLEESNQANQQQLWDP-NT----YGRQQPQ 194
Type_III
MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSN 50
RGKLFEFCSSSSMLKTLERYQKCNYGAPETNVISRETQSSQQEYLKLKAR 100
VEALQRSQRNLLGEDLGPLNIKELEQLERQLDASLRQIRSTRTQYMLDQL 150
TDLQRR-EQMLCENKALKRRLEESNQANQQQLWDP-NT----YGRQQPQ 194
M.acuminata_MADS2
MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIVFSN 50
RGKLYEFCSSSSMLKTLERYQKCNYGAPETNIISREIQTSQQEYLKLKAR 100
VEALQRSQRNLLGEDLGPLSIKELEQLERQLDASLRQIRSTRTQCMLDQL 150
ADLQRR-EQMLCEANKALKIRMDEGNQANQQQLWDP-NAHAVAYCRHQPQ 198