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Carbohydrate Polymers
journal homepage: www.elsevier.com/locate/carbpol
Short communication
a r t i c l e i n f o a b s t r a c t
Article history: Starchy substances constitute the major part of the human diet for most people in the world. a-Amylases
Received 23 September 2007 are widely distributed enzymes that initiate the hydrolysis of starch into low molecular weight malto-
Received in revised form 29 August 2008 dextrins. The effect of pH on the rate of hydrolysis of both sorghum (Montecillo 2 and 3) hybrids starches
Accepted 15 September 2008
by industrial bacterial amylase (IBA) and Aspergillus oryzae a-amylase(AOA) was studied. This result sug-
Available online 22 September 2008
gests that both IBA and AOA a-amylases had slightly high affinity for soluble starch of sorghum Monte-
cillo 3 and 1 hybrids, respectively. Using the structural information available, the theoretical pKa values of
Keywords:
its ionizable residues directly involved in the catalytic region were determined. Both our experimental
Aspergillus oryzae amylase
Industrial bacterial amylase
data and prediction studies indicated that the average pKa values are in good agreement.
a-Amylases Ó 2008 Published by Elsevier Ltd.
Sorghum starches
Acknowledgement
References