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TION OF POTASSIUM
BY H. R. D. JACOBS* AND WILLIAM S. HOFFMANi
(From the Department of Medicine, Rush Medical College, the University oj
Chicago, Chicago)
(Received for publication, August 12, 1931)
The usual method for the estimation of potassium in the blood
and the urine depends, as is well known, upon the precipitation of
the element as the cobalti-nitrite and the subsequent estimation
of one of the constituents of the precipitate. The reducing power
Comment
This method embodies the theoretical advantage of determining
directly a stable constituent of the potassium precipitate. The
procedure is relatively simple. The use of a permanent standard
permits of more accurate comparisons between values obtained
at different times, and serves as a check upon the efficiency of the
precipitating procedure when this is in question.
There are only two accurate measurements to be made in each
det.ermination: that of measuring 1 cc. of the serum to be used
and that of diluting the colored solution to the 6 cc. mark. All
of the manipulations are carried out in the original tube so that
there is no loss from transfers.
Washing the precipitate in alcohol has the advantage that it is
thoroughly freed from the precipitating agent without danger of
loss, since it is insoluble in alcohol. The alcohol also makes the
precipitate cohere well so that the supernatant fluid may be
poured off and the tube drained upside down. It was found that
there was a large amount of cobalt in the first water washing, a
small amount in the first alcohol washing, a trace in the second
alcohol washing, and none in the combined third alcohol washings
688 Calorimetric Estimation of Potassium
are probably protein, but they give up their salt readily and
become translucent flakes which cause no difficulty. They settle
TABLE I
Determinations of Potassium in Known Potassium Solutions
TABLE II
Determinations of Potassium in Human Blood Sera*
Potassium
- T Potsssium
-
Case No. Authors’
KtT
Tisdrtll
Authors’
method
KEY
Tisdall
method
after
method method ashing
_- _.
m7. mg. mg. ?w. m7.
per cent per cent per cent pzent per cent per cent
19.9 17.0 19.7 6 18.6 16.6 17.1
19.9 18.5 19.9 18.7
18.4 19.6 7 19.4 19.4
18.2 19.7 19.0
21.1 8 21.9 19.9
21.3 21.9
21.4 19.9 9 22.3 20.2 21.7
21.4 22.1 20.7
21.3 20.2 10 20.6 19.8 20.0
21.3 20.9
- - - -
* Ten consecutive cases.
out after the colored solution is formed and remain in the tube
when the solution is poured into the calorimeter cup.
690 Calorimetric Estimation of Potassium
mp. per cent mg. per cent mg. per cent mg. per cent
178.1 24.0 202.1 204.1
178.1 48.0 226.1 226.2
178.1 72.0 250.1 257.6
178.1 96.0 274.1 277.4
The known amounts of potassium were added to measured portions of
the urine, the mixtures then ashed, and the potassium determined in an
aliquot of the solutions of the ashes. Two cobalt standards, one equivalent
to 18 mg. of K per 100 cc., and the other to 36 mg. of K per 100 cc., were used.
TABLE IV
Determinations of Potassium in Serum with Added Potassium
Solution A
1. Kramer, B., and Tisdall, F. F., J. Biol. Chem., 48, 339 (1921).
2. See Briggs, A. P., J. Biol. Chem., 67,351 (1923).
3. Fiske, C. H., and Litarczek, G., J. Biol. Chem., 87, p. xvi (1926).
4. Breh, F., and Gaebler, 0. H., J. Biol. Chem., 87, 81 (1930).