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  • Enzymes Concentration

    Încărcat de

    Ernest Siao
    71 vizualizări2 pagini
    The viscosity of a fixed volume of gelatine can be determined by the time taken for the jelly to drain through a funnel. If the gelatine is treated with the enzyme pepsin this will give a measure of the breakdown of the protein by the enzyme.

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    The viscosity of a fixed volume of gelatine can be determined by the time taken for the jelly to drain through a funnel. If the gelatine is treated with the enzyme pepsin this will give a measure of the breakdown of the protein by the enzyme.

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    Attribution Non-Commercial (BY-NC)
    Descărcați ca PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    71 vizualizări2 pagini

    Enzymes Concentration

    Încărcat de

    Ernest Siao
    The viscosity of a fixed volume of gelatine can be determined by the time taken for the jelly to drain through a funnel. If the gelatine is treated with the enzyme pepsin this will give a measure of the breakdown of the protein by the enzyme.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    din 2

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    PRACTICAL- ENZVlViE CONCENTRA'l'ION AND RATE OF


    REACTION 0 (j

    I~ ~() /
    AIM: To investigate the effect or a reduction in enzyme concentration on the rate of reaction by
    measuring the initial rate of reaction,
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    ~

    BACKGROUND: the viscosity of afixed volume!of gelatine cail be determined by the time
    taken for the jelly to drain through a funnel. if the gelatine is treated with
    the enzyme pepsin and the viscosity determined this will give a rneasure
    C?fthe breakdown (l the protein by fhe enzyme.
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    HYPOTHESIS: (what do you think will be the relationship ~etween protease.concentration


    and the rate of breakdown of protein in gelatine? Use scientific ideas to
    support your prediction).
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    APPARATUS LIST: /
    . 75g of2% gelatine solution (fully set) pH 2.8 bufTer pepsin solu~ion (5%)
    . Funnel 2 pipcttcs
    1
    stopclock test tubes
    41 Test tube rack spatula. 2 X 250cm3 beakers
    41
    Glassware for diluting enzymcs eye protection

    METHOD

    1. Suspend a funnel over an empty beaker.

    2. Pipette 3cm3 of pH 2.8 bufTcr into a test tubc and place in a test tube rack.

    3. Pipette 7cm3 of5% pepsin solution into the test tube.


    4. Stm1thc stopclock as the mixture in the test tube is poured into a beaker containing 75g
    of gelatine. Stir the contcnts gently for 15 seconds using a spatula then leave for 5
    minutes to stand.

    5. Pour the mixture into the funnel using the spatula to empty the beaker.

    6. Restart the stopclock as the first drip Icavcs the funnel. Rccord the time taken for all
    the gelatine to pass through the funnel.

    7. Repeat the test twice more and calculate an average time.

    8. Repeat the experiment with a range of different enzyine concentrations by diluting the
    5% pepsin solution (see below).
    /
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    Enzyme conc % .0 1 2 I 3 4 5
    V01 of 5% pepsin sol/cm-:1 0 1 2 J 4 5
    Vol of distilled water/cmJ 5 4 J 2 1 0
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    RISK ASSESSMENT (identify any risks and list safety precautions) /:


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    RELIABILITY AND ACCURACY OF RESULTS (how will you ensure that the results are
    accurate and reliable? How will you ~nsure that mcasurements arc precise, accuraFe and
    repeatable? Include replicates and lIse of a control).
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    d' Use::-- SV\.VVLe.- 0C)\0~ c1 G4'\~~/ pH <XVl~s.L{6s~~

    IDENTIFICATION OF DEPENDENT AND INDEPENDENT VARIABLES

    1. DEPENDENT VARIABLE =:= C0~1\ C ' c1 ; Q.Y"1-~tJ 0u+ . ;


    COv1C
    2. INDEPEJ\TDENTVARIABLE =:= p~'7'1,/ pv-e-ss,C\...v-I2-./~p~ S\A..bjt.
    3. OTHER VARIABLES =:= 8\~,

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