PAULINE ARCHAMBAULT

57 Patten Road
Tewksbury, MA 01876
978-851-6050 pab3f1e8
@westpost.net

LABORATORY TECHNICIAN
PROFILE
An Innovative, conscientious, and detail oriented worker who is capable of multi
tasking. Capable of motivating and mentoring diverse professional and non-skill
ed teams by leading through example, as well as a dedicated team member.
CORE COMPETENCIES
Tissue Culture; Production, Process Development, and Quality Control * GMP
and OSHA Training
Microscope * Centrifuge * PH meter * Spectrophotometer * Gel Electro
phoresis * Media Prep
Research & Development; Tested Products and Recorded Results * Created Cha
rts
Porometer * Scanning Microscope * Micrometer * Cou
lter Counter

EDUCATION:
Certificate in Biotechnology Minuteman Technical School, Lexington, MA
03/2010 - 06/2010
Skills Attained: SOP writing, Use of sterile technique, Validation procedures,
Calibration and Use of PH meter, Growth and Identification of Bacterial Cultures
,
Growth of Animal Cell Cultures, Preparation of Solutions, Assays, ELISA
Protein purification; PAGE and Column Chromatography.
Bachelor of Arts Merrimack College, North Andover, MA
05/2002
Certificate in Biotechnology Middlesex Community College, Lowell, MA
12/1999

EXPERIENCE
Bio Technical Consultant 2005 to 2010
An independent Subject Matter Expert, available for consultation
as a Bio Technician for a wide range of tasks, specialized functions
and industry specific procedures
SELECT CONSULTING ENGAGEMENTS INCLUDE:
Research Technician
* Tested membrane for thickness, tensile strength, porosity, and flow time
* Lyophilized membrane to make it hydrophilic
* Assisted with fabrication of new membrane
* Mixed chemicals to make lacquers
* Recorded test results and created reports
Pauline Archambault Page 2
Bio Technical Consultant continued
Production Associate - Cell Culture
* Tested protein secreted from cells for concentration and purity
* Cleaned and setup equipment to grow cells
Calibration Technician
* Inspected and calibrated instruments used to check amount of antibiotics in mi
lk products
Production Technician
* Inspected and tested blood gas analyzers
* Inspected Cerezyme
MatTek Corporation Ashland, MA 2000 - 2005
Cell Culture Technician
Tested cells for viability using trypsin, dye, and Spectrophotometer. Removed ce
lls from plate through trypsonization, counted them using a hemacytometer, and d
iluted them appropriately, as well as reviewed Standard Operating Procedures for
readability and preciseness.
Made media and fed cells, prepared supplements, proteins, vitamins, and cell gro
wth factor. Additionally, tested supplements used in production process and figu
red best amount needed and it's' effectiveness.
Prepared inserts for seeding; coated them with gel, treated with NH4 and HCL.

Beth Israel Medical Center Boston, MA 1998 - 1999

Administrative Assistant
Lifeline Company Cambridge, MA 1997 - 1998
Administrative Assistant

SUPPLEMENTAL EDUCATIONAL EXPERIENCE

Tissue culture - growth of malignant, suspension cells- Monoclonal antibody prod
uction,
Characterization of bacterial unknowns and stock cultures
Molecular Biology Techniques- pGLO Transformation, pGLO Plasmid Preps, DNA Finge
rprinting,
Sequencing and Cloning of Recombinant DNA