Irena Malkovska, PhD

Address: 1220 Blair Mill Rd, Apt 709, Silver Spring, MD 20910
imf78264@westpost.net
240 638 6527
Nationality: U.S. citizen

Profile
Seeking administrative/science/government position. Ph.D. in neuroscience. Exc
ellent interpersonal skills acquired through teamwork with scientists and admini
strative personnel from diverse backgrounds. Advanced analytical, problem-solvi
ng, multi-tasking and organizational skills. Strong verbal and writing skills a
cquired through presentations of scientific data, and manuscript and grant writi
ng. Hardworking, goal-focused and detail-oriented.

Work-related skills
* Basic administrative skills
* Planning, problem solving and data analysis in relation to complex scientific
projects
* Writing scientific progress reports, manuscripts and grant applications
* Delivering scientific presentations at meetings
* Expertise in using National Center for Biotechnology Information (NCBI) databa
ses
* Training new staff
* Computer skills: PC, Mac, Word, Excel, PowerPoint, Outlook, Photoshop

Work experience
5/2008-7/2010 Administrative positions with temporary staffing agencies (Kelly)
Organization: Montgomery County Department of Health and Human Services
Data analysis and data entry. Monitored compliance with state regulations.
4/2007-8/2007 Postdoctoral fellow in the laboratory of Dr. Alan Wells, Departmen
t of Pathology, University of Pittsburgh. Performed basic cancer research. Wro
te grant proposal (Prostate Cancer Research Program Award).
11/2005-11/2006 Postdoctoral fellow in the laboratory of Dr. Gonzalo E. Torres,
Department of Neurobiology, Univ. of Pittsburgh. Performed basic neurobiology r
esearch. Wrote grant proposal (Ruth L. Kirschstein National Research Service Aw
ard).
07/1998-07/1999 Technician in the laboratory of Dr. Matyas Sandor, Department of
Pathology, University of Wisconsin-Madison. Performed basic microbiology resea
rch.
08/1996-12/1997 Intramural Research Training Award (IRTA) granted by the Nationa
l Institutes of Health, Bethesda, Maryland. Performed basic science research in
the laboratories of Dr. Kevin Brown and Dr. Fran Hakim.
*gaps in employment due to back problems and back surgery

Education
08/1999-08/2005 Ph.D. in Neuroscience in the laboratory of Dr. Luis F. Parada, C
enter for Developmental Biology, Univ. of Texas-Southwestern Medical Center. GP
A=3.0
06/1993-05/1996 B.S. in Biochemistry from University of Wisconsin-Madison. GPA=
3.4
08/1992-03/1993 Undergraduate student at Cornell University, Ithaca, NY. GPA=4.
0

Language skills
* Fluent in Czech
* Proficient in French

Awards
* IRTA: Intramural Research Training Award granted by the National Institutes of
Health (1996-1997).
* NINDS: National Institute of Neurological Disorders and Stroke postdoctoral tr
aining grant (2005-2006).

Publications
1. Green SW, Malkovska I, O'Sullivan MG, and Brown KE (2000). Rhesus and pig-ta
iled macaque parvoviruses: identification of two new members of the erythrovirus
genus in monkeys. Virology 269:105-112.
2. Hogan LH, Macvilay K, Barger B, Co D, Malkovska I, Fennelly G, and Sandor M (
2001). Mycobacterium bovis strain bacillus Calmette-Guerin-induced liver granul
omas contain a diverse TCR repertoire, but a monoclonal T cell population is suf
ficient for protective granuloma formation. J Immunol 166:6367-6375.
3. Malkovska I, Kernie SG, Parada LF (2006). Differential expression of the four
untranslated BDNF exons in the adult mouse brain. J Neurosci Res 83:211-21.
4. Tran PV, Afana SF, Malkovska I, Dallman MF, Parada LF and Ingraham HA (2006).
Reduction in SF-1 diminishes hypothalamic BDNF expression and results in diet-
induced obesity in a social setting. Cell Metabolism 498:637-48.
5. Monteggia LM, Luikart B, Barrot M, Theobold D, Malkovska I, Nef S, Parada LF,
Nestler EJ (2007). Brain-Derived Neurotrophic Factor Conditional Knockouts Sho
w Gender Differences in Depression-Related Behaviors. Biol Psychiatry 61:187-197
.
6. Online publications with Demand Media Studios (livestrong.com)

Laboratory Skills

Molecular biology: cloning, PCR mutagenesis, RT-PCR, immunoprecipitation, Weste

rn blotting, Southern blotting, Northern blotting, RPA, generation of GST-fusion
proteins, GST pull-down assay.
Tissue culture: culture of COS-7 cells, HEK cells, PC12 cells, embryonic cortica
l neurons, DU-145 cells; transfection.
Histology: paraffin sectioning, cryostat sectioning, vibratome sectioning, in si
tu hybridization, immunostaining.
Bioinformatics: Identified evolutionarily conserved regions (ECRs) of BDNF and p
utative transcription factor binding sites: used programs including NCBI, Ensemb
l, ECR, zPicture, rVISTA etc.
Other: ELISA, vesicular monoamine uptake assay.

Other activities
* Writing: writer with Demand Media Studios
* Painting
* Volunteer with DC Cares
* Volunteer with Meriter Hospital, Madison Wisconsin

References
Dr. Luis F. Parada: Professor and Chairman Center for Developmental Biology, UT
Southwestern Medical Center
Phone: 214 648 1822 E-mail: Luis.Parada@UTSouthwestern.edu
Dr. Steven G. Kernie: Associate Professor Pediatrics, UT Southwestern Medical Ce
nter
Phone: 214 648 4183 E-mail: Steven.Kernie@UTSouthwestern.edu
Dr. Carole R. Mendelson: Professor Biochemistry, UT Southwestern Medical Center
Phone: 214 648 2944 E-mail: Carole.Mendelson@UTSouthwestern.edu
Dr. Eric J. Nestler: Professor and Chair Neuroscience, Mount Sinai School of Med
icine
Phone: 212 659 5656 E-mail: Eric.Nestler@mssm.edu