Mark Scheme Biology Paper 3 (4551/3)

MARKING SCHEME MID-TERM EXAMINATION BIOLOGY PAPER 3 1 (a) [ KB0603 Measuring Using Number]
Score 3 Criteria Able to record 4 readings of time taken in Table 1 correctly: Sample answers Sample M N O P

Time taken

15 s 30 s 55 s 80 s

2 1 0

Able to record 3 correct and 1 incorrect answer . Able to record 2 correct and 2 incorrect answer. Able to give one number, no response or wrong response

1 (b) (i) [KB0601 - Observation]

Score Criteria Able to state any two observations correctly according to 2 criteria: 3 y Water sample ( Manipulated Variable) y Time taken (Responding Variable) Sample answers: 1. In sample M, the time taken for methylene blue solution to decolourised is 15 s 2. In sample P, the time taken for methylene blue solution to decolourised is 80 s 2 Able to state any one observation correctly. or Able to state any two incomplete observations ( any 2 criteria) Sample answers: 1. In sample M/P, the time taken for methylene blue to decolourised is the shortest/longest. 1 Able to state any one idea of observation.(any 1criteria) Sample answers:

1. The time taken is different for the different water sample.

Or any other suitable answer. 0 Not able to response or wrong response.

1 (b) (ii) [KB0604 - Making inferences] Score Criteria Able to make one logical inference for each observation based on the criteria 3 4551/3 Hak cipta Sekolah Berasrama Penuh, 2008 [Lihat sebelah

Mark Scheme Biology Paper 3 (4551/3)

y y

Level of water pollution. The time taken.

Sample answers: 1. In sample M, the time taken for methylene blue to decolourised is

shortest because the water sample is most polluted.

2. In sample P, the time taken for methylene blue to decolourised is

longest because the water sample is less polluted/ clean.

2 Able to make one logical inference for any one observation . or Able to make one logical and incomplete inference base on one criterion for each observation. Sample answer: 1. In sample M/P the level of pollution is higher/lower to show the water

is clean/ dirty.
2. In sample M/P the time taken in shortest/highest because of

clean/dirty.
1 Able to make an idea of inference with one criterion. Sample answers 1. Water pollution is higher/lower 2. Different water sample has different time taken.

Or any suitable answer.

0 Not able to response or wrong response.

1(c) [KB061001 Controlling Variables] Variables Manipulated: Samples of water How the variables are operated

Use 4 samples water from different sources.

Responding:

Time taken
Fixed: Amount of methylene blue

Record the time taken for methylene blue to decolourised using stopwatch. Use the same amount of methylene blue for each sample.

1(d) KB0611- Making Hypothesis] Score Criteria 3 Able to state a hypothesis to show a relationship between the manipulated variable and responding variable and the hypothesis can be validated, based on 3 criteria: 4551/3 [Lihat sebelah

Mark Scheme Biology Paper 3 (4551/3)

y y y

Water sample (manipulated variable ) Time taken (responding variable ) Relationship

Sample answer : 1. The shorter the time taken, the water sample is most polluted, hence the B.O.D value is high. 2. The longer the time taken, the water sample is clean, hence the B.O.D value is low. 2 Able to state less accurate hypothesis to show a relationship between manipulated variable and responding variable base on 2 criteria. Sample answer 1. The shorter the time taken, the more polluted of water. 2. The higher/shorter B.O.D level, the water is more polluted/clean. 1 Able to state idea of hypothesis to show a relationship between manipulated variable and responding variable base on 1 criterion. Sample answer 1. Level of water pollution is varied. 0 Not able to response or wrong response.

1(e) (i) [KB0606 Communicating] Score 3 Criteria Able to draw and fill a table with all columns and rows labeled with complete unit Sample answers

Water sample M N O P
2 1 0

Time taken for colour changes of water sample (s) 15 30 55 80

Able to draw a table with incomplete data Able to draw a table without data Not able to response or wrong response.

1(e) (ii) [KB0608 Interpreting Data] Score Criteria 3 Able to state clearly and accurately the relationship between the time taken and the amount of dissolved oxygen based on the criteria: y P1- the time taken. y P2- B.O.D value 4551/3 [Lihat sebelah

Mark Scheme Biology Paper 3 (4551/3) Sample answer:

1. Rapid decolourisation of methylene blue solution show the lower concentration of dissolved oxygen , hence the B.O.D value is high.
2 1 0 Able to state clearly but less accurate the relationsh ip. Able to state the idea of the relationship . Not able to response or wrong response.

(f) [KB0605] [predicting ] Score Criteria 3 Able to predict the result accurately base on 2 criteria. y The time taken. y The amount of oxygen. Sample answer:

1. The time taken for methylene blue to decolourised is more than 55 s because the amount of dissolved oxygen is high. Methylene blue is used to analyse the presence of oxygen in water. The more dissolved oxygen in water the colour of methylne blue is decolourised slowly.
2

Able to predict result less a ccurate. 1. The time taken for methylene blue to decolourised is more than 55 s. Able to give idea of the result. 1. The time taken is longer.
Not able to response or wrong response.

(g) [KB0609 define operationally ] Score Criteria 3 Able to define operationally base on 3 criteria

y y y

Amount of oxygen. Decolourisation of methylene blue. Time taken

Sample answer:

1. B.O.D means the more/longer time taken for the methylene blue decolorized, the water sample is less polluted/clean because the amount of oxygen is high.

Able to predict the result less accurate base on 1 criteria Sample answer:

1. A high B.O.D value means the time taken for the methylene blue to decolourised is rapid/shortest.
1 Able to give idea of the result

1. The shortest the time taken, the more polluted the water.
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Not able to response or wrong response.

(h) [KB0602 Classifying] Score Criteria 3 Able to state the relationship based on the 2 criteria

y y

Level of pollution Time taken

Sample answer:

1. Slow decolourisation of the methylene blue shows that the B.O.D value is low and the water is less polluted.
2 Able to state relationship less accurate base on 1 criteria Sample answer: 1. . Slow decolourisation of the methylene blue show the water is clean. Able to give idea of the result 1. Different sample of water affect the time taken Not able to response or wrong response.

Score Criteria 3 Able to classify all 3 pairs of the time taken and level of pollution. Sample answer

Sample Station A Station B Station C

2 1 0 Able to classify 2 pairs Able to classify 1 pairs

Time taken 2 hour 1 hour 4 hour

Level of pollution Polluted Very Polluted Clean

Not able to response or wrong response.

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Mark Scheme Biology Paper 3 (4551/3)

6 Score 3

Aspect

Criteria Able to state the problem statement correctly : C1 : Manipulated Variable C2 : Responding variable R : Question form and have relationship Sample Answer : 1. What is the effect of starch / substrate concentration on the rate of amylase reaction / activity ? 2. How starch / substrate concentration affects the rate of amylase reaction / activity ?

Problem statement

# Without question mark (?) score 2 Able to give a statement of identified problem but incomplete. Sample Answer: 1. What is the effect of starch / substrate concentration on amylase? 2. How does starch affect the rate of reaction / activity of amylase? Able to give idea of a statement of identified problem. Sample Answer: 1. What is the starch / substrate concentration? 2. Starch / substrate concentration affects amylase. No response or wrong response

0 Score 3

Aspect

Criteria Able to state the hypothesis correctly by relating two variables. Criteria set: C1 : State the manipulated variable C2 : State the responding variable R: Show the specific relationship and direction between the manipulated variable and the responding variable. Answer must have C1, C2 and R

Sample Answer : 1. The rate of amylase / enzyme reaction /activity increases with the increase in starch / substrate concentration (until it reaches a maximum rate). Hypothesis 2. As the concentration of amylase / enzyme increases, the time taken for the hydrolysis of starch to be completed increases. Able to make a statement of hypothesis which relates the manipulated variable to the responding variable. Answer must have C1 and C2 but without correct relationship Sample Answer : 1. Starch / Substrate concentration increases the rate of amylase / enzyme reaction / activity. 2. Starch / Substrate concentration affects the rate of amylase / enzyme reaction / activity. Able to state an idea of a statement of hypothesis. Sample Answer:
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Mark Scheme Biology Paper 3 (4551/3)

1. The rate of amylase / enzyme reaction / activity increases. No response or wrong response. Aspect Criteria All three variables must be correct : Manipulated variable : Concentration of starch suspension Responding Variable : Time taken for the hydrolysis of starch to be completed / the mixture stops turning blue black in colour when tested with iodine solution // rate of salivary amylase / enzyme reaction / activity Constant Variable : Amylase / Enzyme concentration // temperature Any two variables correct Any one variable correct Criteria Able to state all the materials and apparatus Note: Must have test tubes, thermometer, salivary amylase, iodine and starch suspension to get a tick. Sample answer: Apparatus: test tubes, stopwatch, syringes, glass rods, white tile with groove, droppers, thermometer Materials: salivary amylase, iodine solution, starch suspensions at different concentrations (0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%), distilled water 7A +4M Sample answer: Apparatus: test tubes, stopwatch, syringes, glass rods, white tile with groove, droppers Materials: salivary amylase, iodine solution, starch suspensions at different concentrations (0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%) 6A +3M Sample answer: Apparatus: test tubes, stopwatch, syringes, glass rods, white tile with groove Materials: salivary amylase, iodine solution, starch suspensions at different concentrations 5A +3M No response or wrong response Criteria Able to state all the steps in carrying out the experiment successfully.

0 Score 3

Variables

2 1 Score 3

Aspect

Materials and apparatus

0 Score

Aspect Procedure
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Mark Scheme Biology Paper 3 (4551/3)

K1: Steps to set up the apparatus (any 4) K2: Steps to handle fixed variables (any 1) K3: Steps to handle manipulated variables K4: Steps to handle responding variables K5: Precautionary steps/steps taken for accurate results/ readings (any1) Scoring rubric All K1-K5 present Any 3-4K present Any 1-2K present No response or wrong response

3 2 1 0

Sample answer: K1 K2 +K5 Procedure Six test tubes are labelled A to F. 5ml of starch suspensions of different concentration are poured into the following test tubes using different syringes. A: 0.1% of starch suspension B: 0.2% of starch suspension C: 0.3% of starch suspension D: 0.4% of starch suspension E: 0.5% of starch suspension F: 0.6% of starch suspension 3. The test tubes are immersed in water bath at 37C. 4. Drops of iodine solution are added separately onto the grooves of a white tile using a syringes. 5. 1ml of 0.1% amylase is added to test tube A. 6. Stopwatch is activated immediately. (0 minutes) 7. The contents are stirred with a glass rod. 8. A drop of mixture is tested with iodine solution on the white tile. 9. At every sampling the dropper must be rinsed with clean distilled water. 10. The steps are repeated at 30 second intervals until the mixture stops turning blue black in colour when tested with iodine solution. 11. Time taken for the hydrolysis of starch to be completed is recorded in the table. 12. Steps 4-11 are repeated with test tubes B,C,D,E and F. 1. 2. 13. A graph of the rate of amylase activity against starch concentration is plotted. 5K = 3m 3-4K = 2m 1-2K= 1m
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Notes

K2 K1 K2 K1 K1 K1 K5 K5 K4 K1 +K4 K1

Mark Scheme Biology Paper 3 (4551/3)

Aspect

Criteria Able to draw a complete table to record the relevant data base on the three criteria: -Column for manipulated variable with correct unit: concentration of starch suspension (%) -Column for responding variable with correct unit: Time taken for the hydrolysis of starch to be completed (minute) 1 (minute-1) // graph -Rate of enzyme reaction = Time Time taken for Concentration the hydrolysis of starch of starch to be suspension (%) completed (minute) 0.1 0.2 0.3 0.4 0.5 0.6 Rate of enzyme reaction = 1 Time (minute-1 )

Score 2

Presentation of data

Test tube

A B C D E F

Able to state any ONE criteria No response or wrong response. Total mark = 17 marks

1 0

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