Porphyromonas gingivalis Agar (P.

GING)

Porphyromonas gingivalis agar (P. GING) is an enriched selective medium for the isolation and presumptive identification of Porphyromonas gingivalis. Columbia Agar Base is supplemented with sheep blood, bacitracin, colistin and nalidixic acid as selective agents for the isolation of Porphyromonas gingivalis. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Anaerobe Systems PRAS Porphyromonas

Gingivalis Agar (P.GING) AS-6422 View the full product insert: P.GING Insert.pdf Porphyromonas Gingivalis Agar Products Item # AS6422 Description P.GING mono plate Size Price 4 7.85 plates

Formula
Columbia Agar, 42.5 g Hemin (0.1% soln), 5.0 ml Vitamin K1 (1% soln), 1.0 ml Agar, 6.5 g Nalidixic Acid, 15.0 mg Colistin methanesulfonate, 15.37 mg Bacitracin, 10.0 mg Sheep Blood, 50.0 ml Distilled Water, 1000.0 ml

Final pH 7.1 +/- 0.2 at 25 degrees C. Final weight 16.0 +/- 1.6.

Precautions
For IN VITRO DIAGNOSTIC USE only. Approved biohazard

precautions and aseptic techniques should be observed when using this product. This product is for use only by properly-trained and qualified personnel. Sterilize all biohazard waste prior to disposal.

Storage and Shelf Life

Storage: Upon receipt, store at room temperature (13C - 27C) in original container until use. Avoid overheating or freezing. Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container. Shelf Life: 90 days from date of manufacture.

Procedure
Specimen Collection: Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing. Consult appropriate references for detailed instructions concerning collection and transport of anaerobes. Methods for Use: P. GING should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material. Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37oC for 18-48 hours. Additional periods of incubation may be necessary to recover some anaerobes. Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

Limitations
P. GING will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar also be inoculated from clinical specimens to assure growth of all species present. Consult reference materials for additional information.

Quality Control
P. GING should support good growth of Porphyromonas gingivalis found in clinical infections. The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.
Organism Tested Fusobacterium nucleatum Actinomyces viscosus Propionibacterium acnes Porphyromonas gingivalis Actinobacillus actinomycetemcomitans Staphylococcus aureus Clostridium difficile Bacteroides fragilis Porphyromonas asaccharolytica ATCC # 25586 43146 6919 33277 29522 25923 9689 25285 25260 Results No Growth No Growth No Growth Growth No Growth Growth Growth Growth Sparse Growth Time (Hours)

48 72 h 24 48 h 48 h 24 48 h

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user. If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 96 hours. If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

Organism B. fragilis P. gingivalis F. nucleatum

Expected Growth 48 hrs 72 hrs Inhibited

A. viscosus P. asaccharolytica

Inhibited Sparse Growth

Physical Appearance: P. GING should appear opaque red in color.