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Website: www.allelebiotech.com Call: 1-800-991-RNAi/858-587-6645 (Pacific Time: 9:00AM~5:00PM) Email:

Website: www.allelebiotech.com Call: 1-800-991-RNAi/858-587-6645 (Pacific Time: 9:00AM~5:00PM) Email: oligo@allelebiotech.com

For Technical Support:

Email:FP@allelebiotech.com

F or Research Use Only. Not for Diagnostic or Therapeutic Use.

Purchase does not include or carry any right to resell or transfer this prod- uct either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Allele Biotech

is strictly prohibited

authorization of Allele Biotech is strictly prohibited HeLa cells expressing mRFP-PCNA fixed and permeabilized.

HeLa cells expressing mRFP-PCNA fixed and permeabilized. Staining with RFP- Booster_Atto594 (dilution 1/200, 1 h at RT).

with RFP- Booster_Atto594 (dilution 1/200, 1 h at RT). Protocols Chromotek-RFP-Trap® Booster Description

Protocols

Chromotek-RFP-Trap® Booster

Description

Specificity

Relevance

RFP-Trap® coupled to fluorescent dye ATTO 594

RFP-Trap® efficiently highlights monomeric derivates of dsRed - (mRFP1, mCherry,mOrange, mPlum), but not GFP and its variants.

Red fluorescent proteins (RFPs) are widely used as fluorescent tags. Commonly, a combination of red and green fusion proteins (GFPs) is employed to allow multicolor tracking and analysis of protein-protein interactions. In most applications, the ability to observe fluorescence from red florescent proteins is hindered because the quantum yield and photo- stability of RFPs are usually not sufficient enough for their microscopic detection. This limitation is particularly important for Super Resolution Microscopy applications (e.g. 3D-SIM or STED). Many cell biological methods such as HCl treatment for BrdU-detection, the EdU-Click-iT™ treatment or heat denaturation for FISH, lead to disruption of the RFP signal. By using RFP-Booster_Atto594, a specific RFP-binding protein coupled to the fluorescent dye ATTO 594 (from ATTO-TEC), your RFP signal will significantly increase in stability and brightness.

ATTO 594 is a fluorescent label belonging to the class of Rhodamine dyes. Fluorescence is excited most efficiently in the range 560 - 615 nm (λabs= 601 nm), a standard 561 nm laser is also suitable for excitation. Maximum fluore- scence emission falls in the range 615 - 680 nm (λfl= 627 nm).

For further information please refer to http://www.atto-tec.com

Tested Appli-

cations

Immunofluorescence

Tested Appli- cations Immunofluorescence Conc. 1 mg/ml Storage Shipped at RT. Store at 4°C Avoid
Conc. 1 mg/ml Storage Shipped at RT. Store at 4°C Avoid freezing Product List Chromotek-RFP-Trap®
Conc.
1 mg/ml
Storage
Shipped at RT. Store at 4°C
Avoid freezing
Product List
Chromotek-RFP-Trap® Booster
ACT-CM-RBOOSTR
100 ug
List Chromotek-RFP-Trap® Booster ACT-CM-RBOOSTR 100 ug Allele Biotech-Introducing Cost Effectiveness to Research