Lovely Professional University,Punjab

Format For Instruction Plan [for Courses with Lectures and Labs

Course No BTY210

Cours Title FUNDAMENTALS OF BIOTECHNOLOGY

Course Planner 14243 :: Sutanu Samanta

Lectures Tutorial Practical Credits 4 0 0 4

Text Book:

1 Das H.K. ;Textbook of Biotechnology; Wiley Dream Tech India Pvt. Ltd.; 2nd edition; 2005

Other Specific Book:

2 Voet Donal Fundamentals of Biochemistry life at molecular level, 2nd edition, john wiley .inc 3 T. A Brown. Genetics a Molecular Approaches, 3rd edition1998. chapman & Hall. 4 John E Smith; Biotechnology; Cambridge University Press; 3rd edition 5 Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter 6 Molecular Biology of The Cell; Garland Science; 5th edition 7 Michael J. Pleczar. Microbiology 5th edition.Tata McGRAW

Other Reading Sr No Jouranls atricles as compulsary readings (specific articles, Complete reference) 8 An overview of the structures of protein-DNA complexes, Published: 9 June 2000 Genome Biology 2000, 1(1):reviews001.1001.10 9 Molecular mechanism of immune response induced by foreign plasmid DNA after oral administration in mice, World Journal of Gastroenterology, 2007 July 28; 13(28): 3847-3854 Relevant Websites Sr. No. (Web adress) (only if relevant to the courses) 10 ttp://cnx.org/content/m11415/latest/ 1 Salient Features Website encompasses the latest research related to central dogma of molecular biology Approved for Autumn Session 2011-12

11 http://chemgroups.northwestern.edu/odom /ret/RET %20handout%20- %20Christine%20Cha.pdf 12 http://www.iisc.ernet.in/currsci/mar252004/75 8.pdf

Research article related to southern hybridization Merits and demerits of transgenic crops

Detailed Plan For Lectures

Week Number Lecture Number Lecture Topic Chapters/Sections of Pedagogical tool Textbook/other Demonstration/case reference study/images/anmatio n ctc. planned

Part 1
Week 1 Lecture 1 Prospect of Biotechnology ->Reference :2,->>Reference 31/ 3/ 416 ->Reference :2,3/2/4150 http://www.cgiar.org/biot ech/rep0100/ajlouni.pdf

Lecture 2 Lecture 3 Lecture 4 Week 2 Lecture 5 Lecture 6 Lecture 7 Lecture 8 Week 3 Lecture 9 Lecture 10 Lecture 11

Nucleic acid- Heredity material and transforming experiment Nucleic acid- Heredity material and transforming experiment DNA structure RNA structure Central dogma, RNA types RNA types and functions Gene definition and structure Overview of transcription Overview of translation Overview of translation

->Reference :2,23/2/818-828 ->Reference :3,6/3/7184

Animation/D NA strcture.flv

->Reference :2,27/2/1011-1013 ->Reference :3,5/3/5866 ->Reference :3,9/3/133 -136 Animation/ proteinsynthesis .swf ->Reference :1,6/1/393 -395

Lecture 12 Week 4 2 Lecture 13

Mutation definition

Classification based on types of alteration in primary ->Reference :1,6/1/395 DNA sequence -402 Approved for Autumn Session 2011-12

Part 2
Week 4 Lecture 14 Lecture 15 Lecture 16 Week 5 Lecture 17 Lecture 18 Lecture 19 Lecture 20 Week 6 Lecture 21 Lecture 22 Lecture 23 Lecture 24 Week 7 Lecture 25 Lecture 26 Lecture 27 Lecture 28 Classification based on types of alteration in primary ->Reference :1,6/1/395 DNA sequence -402 Classification of mutation based on their effect on the gene function Restriction enzymes Classification of restriction enzymes Overview of recombinant DNA technology Vectors and their properties Plasmids Cloning strategy Concept of PCR Applications of PCR Protein structure, Amino Acid Structure Protein structure, Amino Acid Structure Enzymes and properties of enzymes Applications of enzymes Revision ->Reference :1,16/1/973-975 ->Reference :1,16/1/1000-1002 ->Reference :1,16/1/1002--1010 ->Reference :1,2/1/105 -112 ->Reference :1,2/1/105 -112 ->Reference :2,11/2/313-317 ->Reference :2,11/2/331-332 Animation/ PCR.swf Animation/steps in cloning a gene.swf ->Reference :1,6/1/403 -404 ->Reference :1,16/1/987-991 ->Reference :1,16/1/987-991 ->Reference :1,16/1/973-975 ->Reference :1,16/1/1008-1011

MID-TERM Part 3
Week 8 Lecture 29 Lecture 30 Lecture 31 3 Techniques: Agarose gel, Southern hybridisation Transforming E. coli cells using Cacl2 Microbial media, sterilization ->Reference :2,23/2/843-844 ->Reference :2,23/2/844-845 ->Reference :7,5/4/6671 Approved for Autumn Session 2011-12 Animation/electrophores is.swf animation/ transformation.swf

Week 8 Week 9

Lecture 32 Lecture 33

Microbial media, sterilization

->Reference :7,5/4/6671 ->Reference :7,14/1/863-869

Isolation of pure culture Lecture 34 Lecture 35 Lecture 36 Week 10 Lecture 37 Isolation of pure culture Cells of the immune systems and organs Cells of the immune systems and organs Immunoglobulin types and their functions

->Reference :1,14/1/863-869

Animation/ phagocyt osi.swf

->Reference :1,14/1/871-874

Animation/ IgE mediated hypersenstivity

Part 4
Week 10 Lecture 38 Lecture 39 Lecture 40 Week 11 Lecture 41 Lecture 42 Lecture 43 Lecture 44 Introduction to Bioinformatics Sequence comparison Fermentation overview Basic design of fermenter Ethanol fermentation Ethanol fermentation Application of Biotechnology Application of Biotechnology BT cotton Production ->Reference :3,1/3/416 ->Reference :3,1/3/416 http://www.ars.usda.gov/ is/np/btcotton/btcotton.p df ->Reference :3,1/3/416 http://www.ncbi.nlm.nih. gov/pmc/articles/PMC12 19086/pdf/9445392.pdf http://globalresearchonli ne.net/volume1issue1/A rticle%20009.pdf ->Reference :1,20/1/1307-1313 ->Reference :1,20/1/1313-1315 ->Reference :1,7/4/167 -168 ->Reference :1,7/4/168 -169 ->Reference :1,13/1/703-711

Week 12

Lecture 45 Lecture 46

Lecture 47

Insulin Production

Lecture 48

Revision

Approved for Autumn Session 2011-12

Spill Over
Week 13 Lecture 49 DNA STRUCTURE Watson Crick Model Lecture 50 DNA STRUCTURE Watson Crick Model http://www.ias.ac.in/curr sci/dec102003/1556.pdf http://www.ias.ac.in/curr sci/dec102003/1556.pdf

Details of homework and case studies

Homework No. Objective Topic of the Homework Nature of homework (group/individuals/field work Individual Evaluation Mode Allottment / submission Week 3/3

Class Test 1

Assessing how students understand the courses being taught Assessing how students understand the courses being taught Assessing how students understand the courses being taught

Nucleic acid- Heredity material and transforming experiment,DNA Structure, RNA Structure,Central dogma, RNA types and function,Gene definition and structure

Checking the answer sheets

Class Test 2

Classification based on types of alteration in primary DNA sequence,Classification of mutation based on their effect on the gene function,Classification of restriction enzymes,Vectors and their properties,Plasmid, Cloning strategy

Individual

Checking the answer sheets

6/6

Class Test 3

Techniques: Agarose gel, Southern Individual hybridisation,Bacterial transformation,Microbial media, sterilization,Isolation of pure culture,Cells of the immune systems and organs,Immunoglobulin types and their functions,

Checking the answer sheets

10 / 10

Scheme for CA:out of 100*

Component Class Test Frequency 2 Total :Out Of 3 Each Marks Total Marks 10 10 20 20

* In ENG courses wherever the total exceeds 100, consider x best out of y components of CA, as explained in teacher's guide available on the UMS

Approved for Autumn Session 2011-12

Approved for Autumn Session 2011-12