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What is Nanotechnology? Nanotechnology (sometimes shortened to "nanotech") is the study of manipulating matter on an atomic and molecular scale. Generally, nanotechnology deals with structures sized between 1 to 100 nanometre in at least one dimension, and involves developing materials or devices possessing at least one dimension within that size. (Wikipedia)
Section
Atomic to cellular Atomic to cellular Atomic to cellular
Range
10-15 10-12 10-9
Unit
fm (femto) pm (pico) nm (nano)
Example
Proton, neutron wavelength of gamma rays and X-rays, hydrogen atom DNA helix, virus, proteins, wavelength of optical spectrum
Human scale
Human scale
10-6
10-3
m (micro)
mm (milli)
Nanotechnology Applications: -> Medicine (Diagnostics, Drug delivery, Tissue engineering) -> Environment (Biosensors) -> Consumer goods (food, textiles, household, optics, cosmetics, agrigulture) -> Energy (new materials for energy reduction/increase of efficiency) -> Information and communication (new memory storage, display) -> Heavy Industry (new materials using nanoparticles)
Nanotechnology Applications:
Nanomedicine: Biosensors for diagnostics (blood glucose, blood alcohol,) Drug delivery systems (Cancer therapy, vaccines)
Nanotechnology Applications:
Nanotechnology Applications:
Nanotechnology Applications:
Environment:
-> using and manipulating DNA to make proteins or motify organism (bacteria, fungi, plants or animals)
AFM image of DNA
What is a cell? -> All living organisms are made out of cells -> Cells are the smallest living unit
Yeast - Fungi
Bacteria Human red blood cells Plant cells
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Prokaryotic cells:
Bacteria
Eukaryotic cells:
Fungi (Yeast, mushrooms) Animals Plants
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DNA: Deoxyribonucleic Acid -> codes for all proteins in the cell -> inherited
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RNA
DNA
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DNA
RNA
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DNA:
RNA:
5-ATG-3
5-UGC-3
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DNA -> double stranded (ds) RNA -> single stranded (ss)
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3D structure of DNA
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DNA Replication
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DNA
-----------------> Transcription
mRNA
-------------------> Translation
Protein
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What is a gene ?
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1. Recombinant protein production -> Enzymes produced for medical applications, biotechnology, food, (-> Biosensor) 2. Modified Organisms -> plants with altered properties, transgenic fish,. (-> whole cell biosensor) 3. Study diseases
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Gene Library
DNA fragments are part of the genome -> Library of one organism
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Specific amplification of DNA Can be used to detect disease or infectious agents, modify genes (mutations,), screening of libraries, forensic technology, cloning of genes,.. Key technology of gene technology today !!! Golden standard in Diagnosis:
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1st Cycle Result after 30 cycles -> just DNA between the primers amplified
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GFP variants
Engineering of a molecular spring (light blue, right). Under tension (lower part of the figure) the spring leads to a change in the regulatory subunit which causes the catalytic subunit (purple, left) to separate, activating the enzyme. (Giovanni Zocchi et al, UCLA)
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Escherichia coli
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Transgenic Animals
Production of pharmaceutical proteins -> drugs
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Cloning -> obtaining an organism that is genetically identical to the original organism
-> such as Dolly the sheep
-> asexual propagation of plants (taking cuttings) -> natural clones -> twins
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Viral gene transfer into somatic stem cells -> gene therapy
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DNA Nanostructures
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DNA Nanostructures
A rotaxane: The first DNA nano-device with a freely movable ring. Source: Famulok/University of Bonn
Thorsten L. Schmidt and Alexander Heckel, Nano Lett., 2011, 11 (4), pp 17391742 Box with a lock by D. Benetsen, iNano (Aarhus Univeristy) 37
DNA Nanostructures
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