Makerere University College of Heath Sciences Department of Medical Microbiology MCM 7105: Diagnostic Microbiology and Immunology

TIMETABLE THEORY/TUTORIALS Tutorial Days: Mondays, Time: 2-4pm, Venue: Microbiology Classroom
Date 2011 29 Aug 5 Sept 12 Sept 19 Sept 26 Sept 3 Oct 10 Oct 17 Oct 24 Oct 31 Oct Tutorial Topic Review of basics in microbiology: brief history, branches, and scope International Rules for Nomenclature of Microorganisms Cell Biology, Differences between eukaryotic and prokaryotic cells Normal flora and its role in prevention of infection Applied advanced Bacteriology Applied advanced Virology Applied advanced Mycology Applied advanced human Parasitology Innate and acquired immunity Cells and organs of the immune system Immunogen, Ag & Ab concepts Ag-Ab interaction and their application in disease and diagnostics MHC & HLA T-Cell receptor, Ag presentation and immune response mechanisms Cytokines and cytokine assays The Complement System Immunological mechanisms of Hypersensitivity Auto immunity Tumour and Transplant Immunology Review of basics in Molecular Diagnostics - DNA, RNA, other genetic elements, Probes, Primers, PCR PCR/Molecular Methods for detection of microorganisms /drug resistant microorganisms PCR types & detection of post-PCR products Genetic typing of Microorganisms in Medicine and Epidemiology Mechanism of drug resistance in bacteria, viruses, Parasites and fungi: e.g. B-lactamase resistance, MRSA, ESBL, MDR-TB, XDR-TB, VRE, VISA, resistance to ARVs & anti-parasitic agents Investigation of outbreaks of drug resistant Microorganisms Lab economics issues vs test menu and test algorithm Laboratory Services Policies by level of care in Uganda M. Med Student Tutor M Joloba F Bwanga F. Bwanga D Kateete M Joloba H Kajumbula F. Bwanga H Kajumbula F. Bwanga D Kateete Helen Buteme Helen Buteme Helen Buteme H Mayanja F. Bwanga Grace Ndeezi Mark Kaddu Mukasa Kalyesubula B Asiimwe D Kateete F Bwanga B Asiimwe P Byakika, F Bwanga, Sam Nsobya CF Najjuka Joan Kalyango M Joloba & F. Bwanga

7 Nov 14 Nov 28 Nov 5 Dec 12 Dec

PRACTICALS Practicals in Clinical Laboratory Techniques Collection, Transport, Processing of specimens, and interpretation of reports Practical days: Thursdays. Time: 2-4pm. Venue: Microbiology Labs or alternative
Date 2011 1 Sept 8 Sept Practical Topic Disinfection, Antisepsis, Sterilization and Bio safety in prevention of Lab acquired infections The Diagnostic cycle and interface with Laboratory personnel Quality in Labs - Pre-analytical, Analytical & Post-analytical issues Standard Operating procedures. QA vs QC, Lab Accreditation issues Specimen collection and transport including Lab request form issues Staining and Microscopy techniques in Clinical Microbiology Serology: Immunochromatography, Immunofluorescence, ELIZA, Western blot, Immunoprecipitation, etc Blood cultures CSF, Joint/Abdominal/Pleural fluids Biopsy tissues, Musculoskeletal specimens Urine cultures in the diagnosis of UTI Pus cultures Nasopharyngeal/Oropharyngeal swabs/washes for viruses/bacteria Sputum/induced sputum/BAL for bacteria, fungi & Mycobacteria Genital tract specimens Stool processing for GIT pathogens bacteria and Parasites Fungal cultures Virus diagnostics Antimicrobial Susceptibility testing (AST) - techniques and interpretation of AST reports Agglutination assays use salmonella tests for demos Immunochromatography Use HIV Determine/Urine hCG/ Malaria rapids for demos ELISA use the HepB/Toxoplasma gondii ELIZA or alternative Western blot assay Cytokine assays Flow cytometry assays for CD3/CD4/CD8 counts Separation of peripheral blood Monocyte: Technique & purpose T-cell activation in the Lab Skin tests for infections e.g. tuberculin skin test Tutor F. Bwanga M. Joloba, M. Kamya Christine Nansubuga

15 Sept 22 Sept 29 Sept 6 Oct 13 Oct 20 Oct 27 Oct 3 Nov 10 Nov 17 Nov 24 Nov 1 Dec 8 Dec

F. Bwanga H. Kajumbula, Helen Buteme F. Bwanga, H Kajumbula H Baluku CF Najjuka, H Kajumbula H Baluku F. Bwanga, M. Joloba F Mumbowa, Sam Nyole H Kajumbula, F. Bwanga Waisswa M. F. Bwanga, EL Kibuka E Nakakawa M. Joloba, F. Bwanga H Baluku Irene Biraro Sam Kirimunda, Nakimuli Annette Paul Odong, H Buteme Irene Biraro, Sam Kirimunda Nakimuli Annette Sam Kirimunda Irene Biraro, Sam Kirimunda, Nakimuli Annette

Mid term progressive: Friday 11 Nov 2011. Final exam: College AR to announce the date. COURSE COORDINATOR: Freddie Bwanga, Dept of Medical Microbiology Room C-22, Email: <fxb18@case.edu>, Phone: +256 772 662 876 Or +256 704 662 876.
REFERENCES
Bacteriology 1. Koneman E W. et al (editors). Colour Atlas and textbook of Diagnostic Microbiology. Library of Congress Cataloging-in-Publication Data, Lippincott. 5th ed. 1997 2. Murray PR et al (editors). Manual of Clinical Microbiology. 8th edition 3. Brooks, Biology of Microorganisms Immunology: Goldby AR, Kindt T, Osborne B, Kuby Janis. Immunology 5th or later edns. ISBN: 0-7167-4947-5 Mycology: Larone HD. Medically important Fungi 4th Edition. ASM Press. ISBN: 1-55581-172-8 Other resources Laboratory SOPs available in the teaching labs. Internet sources and electronic journals e.g Journal of clinical Microbiology Consult tutors for other references