F01-1329_ 1_A.

doc
Low Leve| Laser for Iat keduct|on and 8ody keshap|ng - Case
Stud|es w|th the I-||po |aser d|ode system.

u lreeman M.Sc. h.u., L 8eynon-Wllllams
AugusL 2009

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MeJlcol 5etvlces fot tbelt teseotcb coottlbotloos.

Abstract


1he ablllLy of low level lasers Lo cause phoLomodulaLlon of lndlvldual adlpose
cells so LhaL Lhey release Lhelr conLenLs wlLhouL damage Lo Lhe cell or surroundlng
Llssues provlded has been successfully and safely used for several years, flrsL as an
ad[uncL LreaLmenL Lo reduce Lhe posL LreaLmenL swelllng and dlscomforL of llposucLlon
procedures and more recenLly on Lhelr own as a non-lnvaslve body reshaplng LreaLmenL
opLlon.

1he purpose of Lhls sLudy was Lo reporL Lhe ablllLy of Lhe l-llpo laser dlode sysLem
Lo be used as a faL reducLlon and body reshaplng sysLem, offerlng paLlenLs lmmedlaLe
and long Lerm changes Lo speclflc problem faLLy areas. Slngle LreaLmenL observaLlons on
clrcumference measuremenL changes of Lhe abdomen were reporLed for 20 paLlenLs and
a slngle case sLudy was presenLed of a female paLlenL mlddle-abdomlnal reglon durlng a
course of elghL LreaLmenL sesslons over four weeks. lnLerlm resulLs from plloL sLudy
uslng real-Llme ulLrasound Lo make dlrecL observaLlons and measuremenLs of Lhe
subcuLaneous adlpose layer were also presenLed.

CbservaLlons and measuremenLs conflrm Lhe l-llpo laser dlode devlce as an
effecLlve, safe and paln free opLlon for lmmedlaLe and long-Lerm body reshaplng.



Introduct|on

LlghL Lherapy ls one of Lhe oldesL LherapeuLlc meLhods used by Lhe human race,
from PlppocraLes noLlng Lhe favourable effecLs of Lhe suns rays ln laLe 3
Lh
CenLury 8C,
uv-Lherapy ln Lhe medlcal envlronmenL

Lo Loday, when nearly every hlgh sLreeL has a
cllnlc or salon provldlng llghL based LreaLmenL opLlons
(1)(2)
. ln parLlcular hlsLory records
Lhe use of vlslble red llghL for curlng varlous medlcal condlLlons slnce Lhe mlddle ages,
such as when Lhose sufferlng from small pox were cured by belng placed ln rooms
lllumlnaLed only by dlm red llghL whlch fllLered Lhrough Lhlck red curLalns from Lhe
sunllghL ouLslde.

1he blologlcal and physlologlcal effecLs of low level lasers on Llssues was flrsL
evaluaLed ln Lhe 1960's and 1970's ln LasLern Lurope, ln parLlcular ln Pungary and Lhe

F01-1329_ 1_A.doc
SovleL unlon
(3)
. uurlng lLs early sLages laser blo-sLlmulaLlon was vlewed wlLh a greaL
deal of scepLlclsm, Lhe credlblllLy of Lhese early sLudles showlng Lhe dlrecL acLlon of low
lnLenslLy vlslble laser llghL on an organlsm aL Lhe molecular level ln doubL. Slnce Lhen,
low level laser Lherapy (LLL1) has passed Lhrough varlous developmenL phases and
maLured lnLo a vlable LreaLmenL opLlon wlLh much of Lhe early conLroversles no longer
Loplcal, and ls now consldered an everyday parL of llghL Lherapy, from use ln Lhe
physloLherapy world for paln rellef and Llssue repalr
(4)(3)(6)(7)
Lhorough Lo needle-less
acupressure polnL sLlmulaLlon
(8)
.

1he use of LLL1 for as an effecLlve LreaLmenL modallLy for paln rellef was Laken
one sLep furLher ln Lhe early parL of Lhe 21
sL
cenLury. nelra eL al documenLed Lhe ablllLy
of LLL1 Lo emulslfy faL and acceleraLe wound heallng afLer llposucLlon procedures uslng
scannlng elecLron mlcroscopy (SLM) and magneLlc resonance lmaglng (M8l)
(9)(10)
. 1hls
work hlghllghLed Lhe mechanlsm of adlpose cell llquefacLlon wlLh Lhe cell conLenLs belng
evacuaLed Lhrough Lhe pores ln Lhe cell membrane lnLo Lhe lnLersLlLlal space. 1hls
procedure of releaslng Lhe faL prlor Lo Lhe acLual surglcal lnLervenLlon faclllLaLed Lhe faL
exLracLlon by ellmlnaLlng Lhe need for prolonged Lunnelllng and reduced posL LreaLmenL
dlscomforL, swelllng and brulslng.

lollowlng Lhe successful developmenL of a comblnaLlon LLL1 and vacuum
massage sysLem for body reshaplng and cellullLe LreaLmenL ln 2004
(11)(12)(13)
,
Chromogenex LLd. Lhen produced Lhe l-llpo low level laser sysLem for reducLlon and
body shaplng, whlch was launched ln early 2008. 1hls sysLem bullds on Lhe low level
laser asslsLed llposucLlon procedure developed by nelra, offerlng Lhe paLlenL faL
reducLlon and lnch loss wlLhouL Lhe requlremenL for acLual surglcal lnLervenLlon.

1he l-llpo uses 630nm vlslble red laser dlodes, a wavelengLh whlch has been
demonsLraLed ln Lhe early LLL1 work Lo LargeL Lhe mlLochondrla of Lhe adlpose cells
(14)
.
LlghL of 630-660nm absorbs lnLo Lhe CyLochrome-c-oxldase unlL of Lhe proLon LransporL
chaln wlLhln Lhe mlLochondrla, lnLenslfylng Lhe blologlcal processes Lo lncrease hydrogen
and calclum lons (P+ and Ca2+) ouLpuL. 1hls lncreased producL ouLpuL Lemporarlly
changes Lhe chemlcal balance wlLhln Lhe cells. lncreases ln pP lnslde Lhe cell causes
charge dlsLrlbuLlon changes ln Lhe cell walls openlng pores ln Lhrough whlch cellular
conLenLs can pass. Changes ln Ca2+ lon ln Lhe cell sLlmulaLes Lhe producLlon of a llpase
enzyme, whlch breaks down Lhe enclosed Lrlglycerldes lnLo glycerol and faLLy aclds
(13)
.
Compared Lo Lhe large bulky sLrucLure of Lhe sLable Lrlglycerlde molecule LhaL Lhe body
sLores lLs surplus dleLary energy as, Lhe smaller glycerol and free faLLy aclds are now able
Lo be moblllsed Lhrough Lhe pores ln Lhe cell wall vla LransporL proLelns.

Cnce ln Lhe lnLersLlLlal space Lhey are Laken up by Lhe body's lymphaLlc sysLem
and LransporLed Lo Lhe approprlaLe Llssues Lo be Lurned lnLo energy durlng exerclse. 1he
l-llpo also employs separaLe slngle laser dlode probes, whlch are placed on Lhe nearesL
LymphaLlc nodes Lo Lhe LreaLmenL slLe durlng Lhe procedure Lo opLlmlse Lhe lymphaLlc
sLlmulaLlon and movemenL of Lhe cell conLenLs away from Lhe LreaLmenL locaLlon. Cnce
LreaLmenL ls over and Lhe laser lrradlaLlon ceases, Lhe sLlmulus Lo Lhe blologlcal
processes ln Lhe mlLochondrla ls removed and Lhey reLurn Lo Lhelr naLural reacLlon raLe.


F01-1329_ 1_A.doc
1hls mechanlsm of moblllsaLlon of adlpose cell conLenLs ls exacLly whaL Lhe body
underLakes as and when energy demands need Lo be meL. ln Lhese lnsLances, hormones
lnlLlaLe Lhe mlLochondrlal sLlmulaLlon and enzymes released from Lhe braln ln response
Lo reduced dally calorle lnLake or lnsLances of exerclse. 1he l-llpo moblllses Lhe energy
before Lhe body reallses lL needs lL, wlLh subsequenL posL LreaLmenL exerclse uslng Lhese
freed meLabollLes. 1hls mechanlsm allows Lhe paLlenL Lo choose Lhe area of body
reshaplng or LargeL sLubborn faLLy deposlLs LhaL prevlous dleL and exerclse has been
unable Lo shlfL.


1. Mu|t|-part|c|pant study of the effects of s|ng|e treatments w|th I-||po

revlous research has demonsLraLed almosL 100 evacuaLlon of Lhe adlpose cell
conLenLs lnLo Lhe lnLersLlLlal space durlng 6-8 mlnuLes of per-cuLaneous laser lrradlaLlon
(10)
. uue Lo Lhe rapld movemenL of Lhe lymphaLlc sysLem, Lhese conLenLs can be
removed from Lhe LreaLmenL slLe wlLhln a few mlnuLes leavlng behlnd collapsed cellular
sLrucLures whlch wlll now Lake up less subcuLaneous volume. Pence, clrcumference
measuremenLs of Lhe paLlenL before and lmmedlaLely afLer LreaLmenL wlll Lyplcally
demonsLraLe a reducLlon.

1hls reducLlon can Lhen be malnLalned lf an approprlaLe energy demand ls placed on
Lhe body, such as exerclse, LhaL wlll burn off Lhese freed meLabollLes and remove Lhem
from Lhe body permanenLly. lf Lhese meLabollLes are noL burned off durlng exerclse
Lhey are llkely Lo be resLored lnLo adlpose Llssue by Lhe body's naLural mechanlsms and
Lhus lL would be expecLed for Lhe paLlenL Lo regaln Lhe loss ln measuremenLs seen afLer
LreaLmenL.

1hls secLlon presenLs Lhe daLa from 20 separaLe paLlenLs who underwenL a slngle l-
llpo laser procedure on Lhe mlddle abdomlnal reglon. rlor Lo undergolng LreaLmenL all
paLlenLs compleLed a full medlcal hlsLory and gave wrlLLen consenL Lo LreaLmenL.


1reatment Method

1he dlmenslons of Lhe l-llpo LreaLmenL pads are 130mm by 84mm glvlng a
worklng surface coverage area of 109.2cm
2
. Lach pad conLalns 9 40mW 630nm laser
dlodes whlch are dlsLrlbuLed Lo cover and area of 72cm
2
(assumlng LhaL Lhe laser llghL
reLalns sulLable sLrengLh for molecular acLlvlLy wlLh a spread of 10mm lnLo Llssue as
prevlous sLudles have demonsLraLed
(8)(16)(17)
).

Clrcumference measuremenL polnLs were deLermlned wlLhln Lhe paLlenLs chosen
area of mosL concern. 1hree separaLe measuremenLs were made each 4cm aparL Lo
cover Lhe span of Lhe l-llpo LreaLmenL pads and Lhe exacL level of each of Lhese Lhree
measuremenLs was recorded as helghL from Lhe floor aL four separaLe gulde polnLs on
Lhe anLerlor and posLerlor of Lhe abdomen. Marks aL Lhese four gulde polnLs would Lhen
provlde a sLandard placemenL for Lhe Lape measure for pre and posL LreaLmenL
measuremenLs.

F01-1329_ 1_A.doc



llg. 1. SchemaLlc of MeasuremenLs made pre and posL LreaLmenL


1he clrcumference measuremenLs were recorded uslng Lhe same measurlng
Lape, Lhe Myo1ape from AccuflLness, whlch uses a Lorslon sysLem Lo reLracL Lhe Lape
around Lhe area Lo be measured and mlnlmlses measuremenL error from Lhe operaLor.

AfLer measuremenL daLa had been collecLed Lhe paLlenL was made comforLable
on a LreaLmenL couch laylng face up ln a seml-recumbenL poslLlon. 1reaLmenL was
performed by placlng Lhe four LreaLmenL pads flrsL on Lhe lefL slde of Lhe abdomen wlLh
Lhe flrsL pad lylng lengLhways ad[acenL Lo Lhe umblllcus and subsequenL pads ad[acenL Lo
one anoLher along Lhe medlolaLeral axls of Lhe abdomen wlLhln Lhe horlzonLal gulde
polnLs. 1he pads are secured ln conLacL wlLh Lhe abdomen surface wlLh an elasLlcaLed
sLrap. AddlLlonal slngle dlode probes are Lhen secured Lo Lhe superflclal lngulnal
lymphaLlc nodes wlLh mlcropore Lape. 1reaLmenL commenced wlLh laser lrradlaLlon of
Lhls lefL slde of Lhe body for 10mlnuLes. AfLer Lhe 10 mlnuLes Lhe l-llpo auLomaLlcally
reLurns Lo sLandby and Lhe pads were reposlLloned Lo Lhe oLher slde of Lhe abdomen
beglnnlng agaln ad[acenL from Lhe umblllcus and exLendlng around Lhe rlghL slde of Lhe
abdomen wlLhln Lhe gulde polnLs. 1he lymphaLlc probes were also shlfLed by
approxlmaLely 2-3cm lnwards along Lhe groln crease Lo maxlmlse lrradlaLlon of Lhe
lngulnal node clusLers. 1hls slde of Lhe body was Lhen also glven a 10-mlnuLe dose of
laser lrradlaLlon. AfLer LreaLmenL Lhe pads and lymphaLlc probes are removed and Lhe 3
lndlvldual clrcumference measuremenL polnLs are reassessed.

no sensaLlon or lll effecLs were reporLed by any of Lhe parLlclpanLs.


F01-1329_ 1_A.doc
kesu|ts

lull measuremenL daLa for each of Lhe 3 lndlvldual measuremenLs before and
lmmedlaLely afLer LreaLmenL are glven ln Appendlx 1. 1oLal measuremenL loss over Lhe 3
measuremenLs for each paLlenL ls shown below ln Craph 1 hlghllghLlng a mlnlmum loss
of aL leasL 2.23cm up Lo Lhe greaLesL loss seen ln one paLlenL of 12.73cm.

30 of Lhe paLlenLs achleved a LoLal measuremenL loss of beLween 3-3cm whlle 9
ouL of Lhe 20 paLlenLs had a loss of more 3cm or more from Lhls slngle LreaLmenL.

Slmllar daLa ls reporLed from over 200 l-llpo cllnlcal cenLres world-wlde
conflrmlng Lhe l-llpo lasers effecLlveness for lmmedlaLe 'lnch loss' wlLh no reporLed
adverse effecLs durlng of posL LreaLmenL.



2. S|ng|e pat|ent case study undergo|ng a fu|| treatment course w|th I-
||po

1he followlng secLlon descrlbes Lhe case sLudy of a 46 year-old female paLlenL
Lhrough a full LreaLmenL course on Lhe mlddle abdomlnal reglon. LlghL separaLe
LreaLmenLs were performed over a four-week perlod, wlLh Lyplcally Lwo LreaLmenL
sesslons scheduled per week.

rlor Lo LreaLmenL Lhe paLlenL compleLed a 3-day dleL and exerclse dlary Lo
provlde undersLandlng of Lhelr dleLary and eaLlng hablLs. WhllsL Lhe paLlenL was noL
requlred Lo acLlvely reduce calorle lnLake from Lhelr norm durlng Lhe sLudy, cerLaln Llps
for change were suggesLed Lo maxlmlse meLabollc and lymphaLlc healLh such as
lncreaslng waLer lnLake, reduclng caffelne and replaclng some unhealLhy snacks wlLh
healLhler opLlons. Advlce was also glven regardlng Lhe dally Llme schedule of eaLlng such
as noL mlsslng meals or eaLlng laLe ln Lhe evenlng. CLher Lhan Lhls advlce, Lhe effecLs of
ad[usLlng dleL on paLlenL response durlng Lhls sLudy were noL lnvesLlgaLed and Lhe
1oLal Loss over 3 MeasuremenLs from Slngle l-llpo 1reaLmenL
on Lhe Abdomlnal Area of 20 aLlenLs
0
2
4
6
8
10
12
14
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Patient ID
T
o
t
a
l

M
e
a
s
u
r
e
m
e
n
t

L
o
s
s

/

c
m
Total Loss (cm) over 3 Measurements

F01-1329_ 1_A.doc
paLlenL conflrmed LhaL durlng Lhe perlod of sLudy Lhelr dleL had varled llLLle from Lhelr
normal behavlour ln Lhe four weeks prlor Lo Lhe sLudy commenclng.

rlor Lo LreaLmenL commenclng Lhe paLlenL compleLed a full medlcal hlsLory and
gave wrlLLen consenL Lo LreaLmenL.

aLlenL helghL and welghL were measured before LreaLmenL uslng Lhe SLCA S223
lreesLandlng PelghL MeLer and Lhe SLCA S797 LlecLronlc lllar Scales. 8ody faL lndex
was calculaLed uslng laL rack ll ulglLal Sklnfold Calllpers and followlng manufacLurer
guldellnes for accuraLe deLermlnaLlon.

1he measuremenLs for Lhe paLlenL are glven ln 1able 1 below:

PelghL/cm WelghL/kg uress Slze 8ll/
172 89 16 39.0

1able 1. aLlenL SLaLlsLlcs rlor Lo 1reaLmenL



1reatment Method

1he LreaLmenL meLhod used was as descrlbed durlng SecLlon 1.

hoLographs pre and posL LreaLmenL were also Laken from anLerlor, lefL, rlghL
and posLerlor aspecLs. aLlenL poslLlonlng was repllcaLed as fully as posslble wlLh Lhe
paLlenL sLandlng wlLh feeL wlLhln floor markers, wlLh Lhelr hands placed on head and
relaxlng fully Lhe abdomlnal reglon. 1hls poslLlonlng was also used when maklng
clrcumference measuremenLs.

AfLer LreaLmenL Lhe pads and lymphaLlc probes were removed and measuremenL
and phoLographlc daLa collecLlon was repeaLed. Any sensaLlons/observaLlons made by
Lhe paLlenL whllsL undergolng Lhe LreaLmenL were also recorded.

1he paLlenL was Lhen dlrecLed Lo underLake 30-43 mlnuLes of exerclse before Lhe
end of Lhe day. ldeally Lhe exerclse should be cardlo-vascular ln naLure alLhough musL
be wlLhln Lhe capablllLles of Lhe paLlenL.
kesu|ts

lull pre and posL LreaLmenL measuremenL daLa for Lhe paLlenL for all LreaLmenL
sesslons are glven ln Appendlces 2.

1able 2 below deLalls Lhe LoLal LreaLmenL loss and reducLlon ln 8ody laL lndex
afLer elghL LreaLmenLs for Lhe paLlenL.




F01-1329_ 1_A.doc

Measurements 8efore ] cm Measurements After ] cm 8ody Iat Index ]

MeasuremenL
1
MeasuremenL
2
MeasuremenL
3
MeasuremenL
1
MeasuremenL
2
MeasuremenL
3
8efore AfLer
at|ent A 111.3 113.0 114.3 102.3 106.0 108.3 39.0 33.3

1able 2. 1oLal 1reaLmenL MeasuremenL and 8ll Loss


8efore and After hotographs



llg. 3. AnLerlor and LefL Slde vlew
(A) 8efore LreaLmenL, (8) AfLer 8 LreaLmenLs
D|scuss|on

uurlng Lhe course of four weeks, Lhe paLlenL has experlenced a 24cm reducLlon
over Lhree measuremenLs wlLhln Lhe abdomlnal area. Along wlLh Lhls reducLlon Lhey
have reduced Lhelr body faL lndex from 39 Lo 33.3 and Lhelr welghL by 3.3kg. 1hese
changes have been produced wlLhouL dramaLlc change Lo dally calorle lnLake and whlle
exerclse has been underLaken, lL has been resLrlcLed Lo 30-43 mlnuLes, Lwlce per week,
afLer each LreaLmenL sesslon Lo ensure LhaL faL moblllsed durlng Lhe l-llpo LreaLmenL ls
used Lo produce Lhe A1 requlred for Lhe exerclse.


F01-1329_ 1_A.doc
8y uslng Lhe l-llpo, Lhe paLlenL has been able Lo speclflcally LargeL an unwanLed
adlpose deposlL and ensure LhaL Lhls area provldes Lhe meLabollLes subsequenLly burned
off wlLh posL LreaLmenL exerclse. 1hls ls dlfferenL Lo slLuaLlons such as a paLlenL under
very sLrlcL dleLary resLrlcLlons and wlLh vasLly lncreased exerclse reglmes who mlghL
expecL Lo reduce Lhelr welghL and body laL lndex durlng a four week perlod, buL LhaL
Lhls loss would come from unspeclflc areas of Lhe bodles subcuLaneous faL accumulaLlon
dependlng on whlch cells are more senslLlve recepLors Lo Lhe clrculaLlng endocrlne
hormones LhaL LargeL Lhe adlpose cells durlng perlods of energy demand.

1o furLher supporL Lhe observaLlons and evldence of Lhe ablllLy of l-llpo Lo reduce
adlpose volume ln speclflc LreaLmenL areas a plloL sLudy uslng ulLrasound scannlng Lo
measure adlpose layer Lhlckness ls belng underLaken aL Lhe aL Lhe Mldlands ulLrasound
and Medlcal Servlces ln Lhe uk. 1hls sLudy ls uslng real-Llme ulLrasound Lechnlques Lo
vlsuallse and measure Lhe reducLlon ln subcuLaneous adlpose deposlLs ln Lhe laLeral and
mld-lower abdomlnal reglons before and afLer l-llpo LreaLmenL.

lnLerlm concluslons are Lo be fully presenLed aL Lhe LSCAu conference ln nlCL ln
SepLember 2009 buL Lo daLe 20 paLlenLs have been assessed wlLh reducLlons ln faL layer
Lhlckness lmmedlaLely posL-LreaLmenL of 30-30 and no reporLs of slde effecLs from
LreaLmenL and an example of Lhe resulLs are shown below.













llg 3. ulLrasound analysls before and afLer l-llpo LreaLmenL


8efore Lhe l-llpo LreaLmenL Lhe Lhlckness of Lhe adlpose layer can measured aL
2.33cm whlch ls reduced Lo 1.60cm lmmedlaLely afLer a slngle l-llpo LreaLmenL.

1hls sLudy ls Lo provlde reference daLa and Lechnlques for a full eLhlcs commlLLee
approved randomlsed double-bllnded sLudy Lo be underLaken laLer ln 2009.







F01-1329_ 1_A.doc
Conc|us|on

1he l-llpo low level laser devlce offers a paLlenL a fasL, non-lnvaslve and paln-free
LreaLmenL opLlon for lmmedlaLe and long-Lerm sub-cuLaneous faL reducLlon of problem
faLLy areas on Lhe body.

CbservaLlons made of clrcumference measuremenLs durlng Lhe slngle one off
LreaLmenLs conflrm Lhe ablllLy of l-llpo Lo provlde an lnsLanL lnch loss effecL on a
LreaLmenL zone.

1he scepLlcal mlnd mlghL conslder LhaL Lhls lnch loss ls purely Lemporary or
perhaps aLLrlbuLed Lo waLer loss provldlng shorL-Lerm compacLlon of Lhe adlpose cells
and as wlLh mosL aesLheLlc lnch loss Lechnlques, lL would be expecLed LhaL effecLs would
be losL afLer 2-3 days. CbservaLlons made over a four-week perlod durlng Lhe sLudy ln
secLlon 2 demonsLraLe conLlnued lnch loss wlLh addlLlonal laser LreaLmenLs. 1he
LargeLlng of Lhe subcuLaneous adlpose layer by Lhe l-llpo ls also conflrmed from Lhe real-
Llme ulLrasound observaLlons. 1hus a course of l-llpo LreaLmenLs ln con[uncLlon wlLh an
approprlaLe dleL and exerclse reglme offers Lhe ablllLy for slgnlflcanL long-Lerm reshaplng
of chosen anaLomlcal areas.


F01-1329_ 1_A.doc
keferences

(1) WalLer !., 1he heallng gods of anclenL clvlllsaLlons, new Paven ress, 1923
(2) 1odd L., aln: PlsLorlcal perspecLlves ln evaluaLlon and LreaLmenL of chronlc paln,
8alLlmore-Munlch: urban & Schwarzenberg 1983
(3) MesLer L., 1he use of Lhe laser beam ln Lherapy, Crv PeLll., 1966, 29, 107(22): 1012-
1016
(4) Walker !., 8ellef from chronlc paln by low power laser lrradlaLlon, neurosclence
LeLLs., 1983, 116, 43: 339-344
(3) 8re Mlller WS., Laser use for paln conLrol and Llssue heallng ln aLhleLlc medlclne,
hyslcal 1her. lorum, 1983, 26: 14-16
(6) MesLer L. eL al, LffecLs of laser rays on wound heallng, Am. !. Surg., 1971, 122: 332-
333
(7) Surlnchak !., Alagu M., 8ellamy 8. eL al, LffecLs of low-level laser on Lhe heallng of full
Lhlckness skln defecLs, Lasers ln Surg. Med., 1983, 2: 267-274
(8) kroeLllnger M., Cn Lhe use of Lhe laser ln acupuncLure, AcupuncLure & LlecLro
1herapeuL. 8es. lnL. !., 1980, 3: 297-311
(9) nelra 8. eL al, Low level laser asslsLed llpoplasLy appearance of faL demonsLraLed by
M8l on abdomlnal Llssue, Am. !. CosmeLlc. Surg., 2001, 18(3): 133
(10) nelra 8. eL al, laL llqulfacLlon: LffecL of low level laser energy on adlpose Llssue,
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F01-1329_ 1_A.doc
Append|x 1

Measurement data for S|ng|e 1reatment Lffects on the Abdom|na| keg|on of 20
|nd|v|dua| at|ents.

Age Male/lemale 1reaLmenL area MeasuremenLs 8efore
/cm
MeasuremenLs AfLer
/cm
1oLal cm loss/
3 measuremenLs
34 l Abdo 110 109 6
112 108
111.3 110.3
60 l Abdo 102 99 3
104 103
107 106
46 l Abdo 101.3 99 12.73
108.23 104
113 107
28 l Abdo 90 89.23 4.23
93.3 93
96.73 93.73
34 l Abdo 94.2 93.3 3.2
99 96.3
97.73 93.23
40 l Abdo 117.3 116 4.73
123 120
121.23 121
32 l Abdo 78.3 77 3
78 73.73
92 90.73
38 l Abdo 122 121 3.3
123.3 122.23
121.3 120.23
38 l Abdo 91 91 2.23
97.3 97
97.23 93.3
28 l Abdo 99 98.73 4.3
107.23 104
109.3 108.3
44 M Abdo 90.3 89.23 7.73
91.3 89
91.73 87.73
47 M Abdo 92.23 91 3.73
92 91
93 91.3
37 l Abdo 99 99 3.23
104 102.3
110.3 108.73
24 l Abdo 100.3 99.3 2.33
104.3 103
100 99.73
32 l Abdo 103.3 102.23 3
108 106.73

F01-1329_ 1_A.doc
103.3 103.3
23 l Abdo 111 107 9
107 104
116.23 114.23
39 l Abdo 82.6 80 6
80.1 77.3
84.4 83.2
19 l Abdo 88.9 86.3 6.9
91 89
91.3 89
44 l Abdo 112.8 110.2 8.3
114.3 111.3
113.3 112.8
37 l Abdo 89 89 4.3
93.3 91.2
93 92.8



F01-1329_ 1_A.doc
Append|x 2

Measurement data for at|ent before and after mu|t|p|e treatments.



Sess|on Measurement 1
8efore
Measurement 1
After
Measurement 2
8efore
Measurement 2
After
Measurement 3
8efore
Measurement 3
After
1 111.3 109.0 113.0 114.0 114.3 114.3
2 110.3 109.3 113.0 112.0 113.3 112.0
3 108.0 108.0 113.0 112.0 113.3 112.3
4 108.3 106.3 111.3 110.0 112.3 111.0
S 107.3 106.0 110.0 110.0 112.3 110.3
6 107.0 106.0 111.0 108.3 111.3 110.3
7 107.0 103.0 111.0 108.3 110.3 109.0
8 103.3 102.3 108.3 106.0 110.0 108.3
9 101.0 98.0 106.0 103.0 109.3 107.0