AP Lab 5: Cell Respiration Gabriela Espinal | 11.29.11 | Period 9 Purpose: 1. Study the effect of temperature on cell respiration 2.

Determine if cell respiration occurs in germinating and non-germinating peas. 3. Compare the rates of cell respiration in germinating and non-germinating peas. Data: Table 1 Peas Germinating Non-germinating Volume (mL) 8.5 mL 8.5 mL

*Table 2 data: My group was told not to test the effects of temperature, but to acquire the data for rate of respiration of a germinated pea (cool) from another group. Table 3 Peas Germinated, room temperature Non-germinated, room temperature Germinated, cool temperature Analysis of Data: Rate of respiration (kPa/s) M = -.0001913 kPa/s M = -.00032185 kPa/s M = 1 kPa/s

Questions: 1. Identify the hypothesis being tested on this activity. The rate of cellular respiration is higher in the germinating peas in a cold temperature than in the beads or non-germinating peas. The cooler temperature in the cold water baths slows the process of cellular respiration in the both germinating and non-germinating peas. 2. This activity uses a number of controls. What conditions must remain constant and why? The constant temperature in the water baths is necessary for stable reading. The constant volume of KOH from tube to tube leads to equal amounts of carbon dioxide consumption. Other controls included the identical equilibration periods for all the respirometers, time intervals between measurements, and glass beads acting as a control for barometric pressure. The beads served to create a controlled volume for both types of peas. 3. Did cell respiration occur in the peas? How do you know? It does occur in the peas. The rates of cellular respiration are greater in germinating peas than in non-germinating peas. Oxygen levels within the tube changed during the time interval. 4. Describe the relationship between the amount of oxygen consumed and time. There was a constant, gradual incline in the amount of oxygen consumed over an interval of time. 5. What is the effect of germination on the rate of cell respiration on peas? Why do think this is? The germinating seeds have a higher metabolic rate and needed more oxygen for growth. The non-germinating peas needed to consume less oxygen for continued subsistence. 6. What is the effect of temperature on the rate of cell respiration in peas? Why do you think this is? Temperature and respiration rates are directly proportional. As temperature increases, respiration rates increase too. 7. Why is the purpose of the KOH in the experiment? The KOH drops absorbed the carbon dioxide. This caused it to precipitate at the bottom of the test tube and no longer be able to effect the readings. We could then exclusively monitor pressure changes due to oxygen consumption. 8. Why did the vial need to be completely sealed around the stopper? This way, no gas could leak out of the vial and no water would be allowed into the vial. 9. a. Cold blooded animals have their body temperature regulated by interactions with the environment. Warm-blooded animals have an internal temperature kept constant by homeostatic mechanisms which ensure their survival. b. One would expect the rate of respiration to be higher in the the 25 g warm-blooded organism at 10 degrees celsius. c. This would be expected, because a small mammal is endothermic. 10. If respiration in a small mammal were studied at both room temperature (21oC) and 10o C what results would you predict? Explain your reasoning. Think about what you found out in Question 9. I would expect the respiration to be higher at 21 degrees because the animal would need to maintain a higher body temperature. Then, the results would decrease at 10 degrees, because the mammal would be required to retain its body temperature at an even lower value in comparison to room temperature. 11. Design an experiment to examine the rates of cellular respiration with peas that have been germinating for different lengths of time: 0, 12, 24, and 36 hours. What results would you expect and why? First, four respirometers could be set up with individual test tubes. Test tube 1 would contain non-germinating peas. Tube 2 holds peas that have been germinating 12 hours. Tube 3 would

contain the peas that germinated for 24 hours. Tube 4 would hold the peas that germinated for 36 hours. All the respirometers should have the KOH added to the bottom. The respirometers should be placed in baths that are all room temperature, just as long as the temperature is constant. The seeds that have not begun germination would consume very little oxygen. The peas that have been germinating for 36 hours will have the greatest amount of oxygen consumption. The other two hours of germination will produce median results.