Practical Manual No.

(Annona cherimola, A. muricata, A. reticulata, A. senegalensis and A. squamosa)

ANNONA

Field Manual for Extension Workers and Farmers

2006

Copies of this handbook including a monograph on this species can be obtained by writing to the address below: Southampton Centre for Underutilised Crops School of Civil Engineering and the Environment or University of Southampton Highfield, Southampton SO17 1BJ United Kingdom International Centre for Underutilised Crops International Water Management Institute (IWMI) 127, Sunil Mawatha Pelawatte Battaramulla Sri Lanka

ISBN: 0854328165 Southampton Centre for Underutilised Crops (ICUC), 2006 Printed at RPM Print and Design, Chichester, England, UK Citation: SCUC (2006), Annona: Annona cherimola, A. muricata, A. reticulata, A. senegalensis and A. squamosa, Field Manual for Extension Workers and Farmers, Unitversity of Southampton, Southampton, UK . This manual was originally prepared by Dr A.C. de Q.Pinto and has been restructured to conform to an agreed format, by D. Jackson. Drawings by L. Jackson.

THE FRUITS FOR THE FUTURE PROJECT

This publication is an output from a research project funded by the United Kingdom Department for International Development (DFID) for the benefit of developing countries. The views expressed are not necessarily those of DFID [R7187 Forestry Research Programme]. A series of underutilised fruits are being researched and this is Practical Manual No. 5 dealing specifically with Annona species.

CONTENTS PART I
PREFACE......................................................................................................................................................... I 1. INTRODUCTION - THE ANNONAS ............................................................................................................1 2. WHY GROW ANNONA TREES?..................................................................................................................1 2.1 NUTRITIONAL VALUE ................................................................................................................................1 2.2 SOCIO-ECONOMIC VALUE ......................................................................................................................... 2 2.3 MEDICINAL USE ...................................................................................................................................... 2 2.4 INDUSTRIAL PRODUCTS ............................................................................................................................ 2 3. WHERE TO GROW ANNONA TREES ........................................................................................................ 3 4. WHAT TO GROW?.................................................................................................................................... 4 5. HOW TO GROW ANNONA...................................................................................................................... 4 5.1 SELECTION OF SCIONS AND ROOTSTOCKS .................................................................................................... 4 5.2 PROPAGATION ........................................................................................................................................ 5 5.2.1 Seed Propagation........................................................................................................................... 5 5.2.2 Vegetative Propagation ................................................................................................................. 5 5.2.3 Budding Method........................................................................................................................... 6 5.2.4 Grafting Method ........................................................................................................................... 6 5.2.5 Other methods.............................................................................................................................. 7 5.3 NURSERY ESTABLISHMENT ......................................................................................................................... 7 5.3.1 Type and Size of Nursery ............................................................................................................... 7 5.3.2 Pots and Seedling Media ............................................................................................................... 8 5.4 FIELD ESTABLISHMENT .............................................................................................................................. 8 5.4.1 Site Preparation ............................................................................................................................. 8 5.4.2 Time of Planting............................................................................................................................ 8 5.4.3 Direct Seeding into the Field.......................................................................................................... 8 5.4.4 Windbreaks................................................................................................................................... 9 5.4.5 Transplanting and Spacing ............................................................................................................. 9 5.5 FIELD MANAGEMENT ............................................................................................................................... 9 5.5.1 Weeding........................................................................................................................................ 9 5.5.2 Irrigation......................................................................................................................................10 5.5.3 Fertilization.................................................................................................................................. 11 5.5.4 Pruning and Training.................................................................................................................... 11 5.5.5 Intercropping and Cover-cropping................................................................................................12 5.5.6 Pollination and Fruit Set ...............................................................................................................12 5.5.7 Pests and Diseases.........................................................................................................................13 6. HOW TO HARVEST ANNONA TREES ......................................................................................................14 6.1 TIME TO FIRST HARVESTING .....................................................................................................................14 6.2 RIPENESS AND YIELD................................................................................................................................14 6.3 HARVESTING TECHNIQUES .......................................................................................................................15 7. POST-HARVEST HANDLING AND PROCESSING .......................................................................................15 7.1 POST-HARVEST HANDLING .......................................................................................................................15 7.2 PROCESSING ..........................................................................................................................................16 8. MARKETING.............................................................................................................................................17 8.1 MARKETING POTENTIAL ...........................................................................................................................17 8.1.1 Constraints to international markets..............................................................................................17 8.1.2 Expected returns ..........................................................................................................................17

BIBLIOGRAPHY ............................................................................................................................................18 APPENDIX I MULTIPLE USES OF ANNONA............................................................................................... 20 APPENDIX IIA MAJOR PESTS OF ANNONA AND THEIR CONTROL .........................................................21 APPENDIX IIB MINOR PESTS OF ANNONA AND THEIR CONTROL ........................................................ 22 APPENDIX IIIA MAJOR DISEASES OF ANNONA AND THEIR CONTROL .................................................. 23 APPENDIX IIIB MINOR DISEASES OF ANNONA AND THEIR CONTROL .................................................. 24 APPENDIX IV - SUGGESTED FERTILISER RATES IN DIFFERENT COUNTRIES ................................................ 24 APPENDIX V - HEALTH AND SAFETY WHEN USING CHEMICALS ............................................................... 25 GLOSSARY ................................................................................................................................................... 26

PART II
Technical Note 1a Technical Note 1b Technical Note 2a Technical Note 2b Technical Note 3 Technical Note 4 Technical Note 5a Technical Note 5b Technical Note 6a Technical Note 6b Technical Note 7a Technical Note 7b Why Grow Annona Trees Where to Grow Annona Trees What to Grow How to Grow Annona - Parts of Annona used for Propagation How to Grow Annona - Propagation by Seed How to Grow Annona - Vegetative Propagation How to Grow Annona - Field Establishment Field Management Harvesting Post-harvest Handling and Storage Processing Marketing and Economics

PREFACE
Fruits for the Future is a programme implemented by the International Centre for Underutilised Crops (ICUC). It is funded by the Forestry Research Programme (FRP) of the UK Department for International Development. ICUC initially implemented this programme in collaboration with the International Plant Genetic Resources Institute (IPGRI), the World Agroforestry Centre (ICRAF) and the Food and Agriculture Organization of the United Nations (FAO). The goal of projects covered by the programme is to improve the livelihoods of the rural poor, particularly those with limited resources, by providing information on high yielding germplasm, high quality planting materials and appropriate technology for environmentally friendly, sustainable natural resources management. Project outputs aim to assist in increasing income from smallholder lands and thus contributing to livelihoods. Until specific projects were organized a major constraint was a lack of accessible practical information for those who advise fruit tree growers, not only on production but also with reference to products and local and regional marketing. This manual summarises important aspects of improved technology and also identifies products and opportunities to improve their marketing. Of course products and their marketing vary both locally and regionally. India, Sri Lanka and Thailand have developed products and marketing strategies for both the local and the international markets; other countries do not possess this framework. A number of people have been involved in the preparation of the manuscript. We would like to take this opportunity to thank them. Dr Alberto C. de Q. Pinto and colleagues prepared the original document. Dr Sue Azam-Ali and Dr Charles R. Clement helped in the editing, along with Angela Hughes of ICUC. David Jackson took responsibility to restructure the manual into an agreed userfriendly format, with illustrations by Ms Lucy Jackson. Any opinions expressed in this manual are those of the people involved in the preparation of the manuscript and not the sponsoring organizations. The techniques illustrated and explained in this manual may not be appropriate for all farmers since the goals and resources of each farmer are different. However, any concept underlying a technique is valid, but the specific techniques, products and market strategies may vary from farmer to farmer and from country to country. We would like to ask those who use this manual to do so creatively as the information to be used practically in the field can result in identifying further research opportunities. Comments and suggestions on the manual are always useful and we look forward to hearing from you. Lastly, we thank DFID-FRP or funding this project, which is part of the programme producing similar outputs on 9 other underutilized fruits. Editors 2005

1. INTRODUCTION - THE ANNONAS

This manual provides practical information on the propagation, management, processing and utilization of 5 species of annona fruits: cherimoya (Annona cherimola), soursop (A. muricata), sugar apple (A. squamosa), custard apple (A. reticulata) and wild soursop (A. senegalensis). Although annonas are widely

cultivated, this manual gives special emphasis to the Latin American region, where much of the genetic diversity exists and where domestication of the first four species listed above took place. The manual is designed to help smallholders shift towards higher-income-generating products and to help those already producing more commercially to become more efficient. Annonas are economically important in many countries of Africa and Asia as well as in South, North and Central America. The name annona derives from the Latin annual harvest. In general, the annonas are shrubs or small trees, with height from 5.0 to 7.5 m; they are erect or somewhat spreading and possess grey-brown bark, often rough and corrugated. With few exceptions, annonas are deciduous, even the tropical species, and especially when cultivated in areas with dry or cool seasons and without irrigation. Annonas are not difficult to cultivate and require comparatively little care. Depending on the species, the commercial life of a healthy annona tree can span 15 years, with yields varying from 8 to 25 t/ha. Cherimoya is the only species that is well adapted to subtropical conditions or tropical highland areas. The other four species are mainly adapted to the tropical lowlands, although they are often grown in subtropical areas. The species with the strongest consumer demand and hence economic production are cherimoya, soursop and sugar apple. The fruits from these three species are delicate and are marketed mainly in local, regional or national trade, and only rarely internationally. Their fruit pulps are sold fresh or frozen, and are usually used as desserts or made into sherbets and ice cream. Although custard apple and wild soursop are less important economically, their fresh fruits are sold in the markets of developing countries.

2. WHY GROW ANNONA TREES?

See Technical Note 1a in Part II. Like many other tropical fruit trees and shrubs, such as mango, coconut and tamarind, the annonas are multiuse plants with acceptable nutritional value as food products. They also serve as sources of medicinal and industrial products (Appendix I). They directly contribute to food security, and provide supplementary household income for small- and medium-scale farmers, as described below.

2.1 Nutritional Value

Annona fruits provide good carbohydrate nutrition, acting as excellent sources of energy. For example, a man weighing 65 kg accustomed to strong physical activity needs 4000 kcal/day in the diet. Since the caloric value of cherimoya is 90 kcal/100 g of pulp, consuming one 0.5 kg fruit each day provides 11% of the daily requirement. Annona fruits are relatively poor sources of proteins and vitamins. They are important sources of minerals, such as calcium, phosphorus and potassium. These are important in biological functions, such as helping to protect bones and teeth, providing strong muscles and improving general health. Cherimoya fruit is considered to have a higher food value than fig, mango and grape.

2.2 Socio-economic Value

Annona trees are generally cultivated in two economic contexts: Custard apple and wild soursop tend to be grown by subsistence farmers under smallholder conditions. Whilst providing nutritional value, they give only a low income to the growers. Cherimoya, soursop and sugar apple tend to be grown on commercial farms as highly remunerative crops for both small and medium scale farmers. In some cases annonas will be cultivated for specific purposes as well as for income generation, for example, sugar apple may be currently grown on wastelands in India for food and health purposes.

2.3 Medicinal Use

Annonas are widely used in traditional medicine in Africa and recently some have been used for production of modern medicines, for example in the USA and India. However, self medication is not recommended. Professional advice from clinics or hospitals should be sought. Annona plant parts used in folk medicine contain chemical compounds such as tannins, alkaloids and flavonoids found in the roots, leaves, bark, fruits and seeds. Acetogenins, for instance, are present in the seeds, roots, bark, stems and fruits of most annona trees, and appear to have great potential in anti-cancer treatments. Cherimoya roots have aporphine alkaloids, which have a relaxant effect. According to traditional folk medicine, the powder of two seeds mixed with water or milk reduces vomiting and tension in the body. Boiled water infusions of leaves help to control convulsions and digestive problems, and also to treat diabetes. The acid pulp of soursop is used to treat foot parasites and liver diseases. Roots of sugar apple can be used to treat acute dysentery and depression while leaves have been used in cases of anal prolapse, sores and swelling. Tea made from roots is highly purgative. Tea from leaves is mildly laxative. Wild soursop roots, leaves and bark are used to treat cancer, convulsions, venereal disease, diarrhoea and dysentery, fever, filariosis and male impotence.

NOTE: ANNONA-BASED HERBAL REMEDIES SHOULD NOT BE USED FOR SELF-MEDICATION. THESE COMPOUNDS HAVE TOXIC PROPERTIES.

2.4 Industrial Products

Cherimoya, soursop, sugar apple and custard apple fruits are widely consumed as processed products, such as ice creams, sherbets, milk shakes, yoghurts, cookies and pastries, syrups and juices. Soursop is the most versatile annona fruit for industrial purposes because it does not oxidize easily and there is a large recovery of pulp from the fruit. Cherimoya can be successfully preserved frozen in a domestic freezer for 120 days. Sugar apple pulp can also be processed and frozen, although its industrial processing is less important than that of cherimoya and soursop.

Annona is also used in the production of essential oils (esters of aliphatic acids) present in the seeds and the fruit pulp. These are used to improve the flavour of processed fruit products. Seeds of soursop can yield about 22% fatty oil for use in flavouring, but this is only about 7% in cherimoya. It is important to note that all extracts from pulp or seeds must be made with high quality control because of the presence of alkaloids and polyphenols, which can be very toxic when concentrated.

3. WHERE TO GROW ANNONA TREES

See Technical Note 1b in Part II. Climate, especially temperature, is an important factor influencing annona orchard location. Table 1 Climatic requirement for annonas Altitude (m) Cherimoya Sugar apple Soursop Tropical highlands From 9002000 Tropical lowlands Up to 800 Tropical lowlands Up to 300 Temperature (C) Summer Dry season 1822 2130 1530 Winter 518 Rainfall (mm) c. 1270 with long dry season 5001000 5001000

Cultivate soursop, custard apple and sugar apple in lowland tropical climates with a temperature between 21 and 30oC. Sugar apple does well when there is a mild winter and it can survive drought, but requires mild summers with evenly distributed moisture for growth and setting of fruits. Rainfall and high humidity during peak flowering season greatly enhances the fruit production of annonas.

The type of soil selected for growing annonas should be carefully considered. Table 2 Suitable habitats for annonas Cherimoya Sugar apple Soursop Soil Characteristics Best on medium soil. May require supplementary calcium and phosphorus. Best on deep soils with good aeration. No water logging. Best on deep well-drained, semi-dry soil. pH 6.57.6 6.0 6.06.5

Custard apple can be cultivated in soils of pH 58 and is tolerant of a range of soil types except for very alkaline soils; consequently, it is recommended as a rootstock for cherimoya and soursop. The selection of the orchard location is also influenced by the availability of local infrastructure and markets. Annona fruits are extremely perishable and cannot withstand transport on poor roads to distant markets unless appropriate packaging is available, such as straw in strong paper bags, woven baskets or cartons, although this extra cost may reduce income. Growers should therefore select a locality near a well made road and as close as possible to a large consumer market.

4. WHAT TO GROW?
See Technical Note 2a in Part II. Before deciding which annona fruit to plant, look at the commercial varieties (cultivars) available in the region because they usually command the best prices at market. There are wide differences among the annona species and cultivars with respect to foliage and fruit yield, as well as size, shape, colour, quality and number of seeds in the fruit. Because of this there exist several cultivars of each annona species.

The most important commercial cultivars for cherimoya, soursop and sugar apple are: Table 3 Important commercial cultivars of annonas Species Cherimoya Commercial Variety Whaley, Pinks Mammoth and Mosman (Australia), Concha Lisa, Bronceada (Chile), Fino de Jete (Spain), White, Bays, Golden Russet, Libby and Lisa (USA), Funchal and Mateus I (Portugal), Burtons Wonder and Reretai (New Zealand), Kabri and Malalai (Israel). Morada, Lisa, Blanca (Colombia), Giant of Alagoas, Selection of Ibimirim, Cerradina (Brazil). Red Sugar Apple (USA), Noi (Thailand), Molate and Lobo (Philippines), IPA Selections (Brazil), Cuban Seedless (Cuba), Barbados and British Guinea (local selections widely propagated), Balanagar, Red Sitaphal (India).

A. cherimola

Soursop

A. muricata
Sugar apple

A. squamosa

5. HOW TO GROW ANNONA

Seed propagation of annonas results in great variability in the orchard and is not recommended. Materials need to be propagated vegetatively to produce more uniform-yielding trees by grafting of the desired variety (scions) onto appropriate rootstocks.

5.1 Selection of Scions and Rootstocks

See Technical Note 2b in Part II. Select rootstock trees that combine the following characteristics: Vigorous and prolific trees with good compatibility for the scion, to produce trees with regular bearing and resistance to cold and dry conditions, pests, and diseases. Mother trees for scion material should have: Well-shaped canopy to facilitate harvesting and minimise pruning. Abundant flowers, which are attractive to insect pollinators. Harvesting period may be out of the main season to avoid market gluts. Fruit with symmetrical form, high natural fruit set, and hard skin to improve pest and disease resistance, as well as to prolong post-harvest life. Excellent fruit quality regarding flavour, with fine, fibre-free and firm pulp texture, seedless or low number of seeds in the pulp.

5.2 Propagation
There are two ways to propagate annona trees: by seed (sexual) and by vegetative (asexual) propagation. Seedlings can be used either as rootstock or as plant material for production. Vegetative propagation involves grafting or budding rootstocks with the desired cultivar to produce trees of uniform genetic characteristics. Seedling trees live longer (about 15 years) than grafted trees (maximum 10 years).

5.2.1 Seed Propagation

Annona seeds rapidly lose their viability, so plant them as soon as possible after removal from the ripe fruits. Germination capacity depends on duration of seed storage, pre-sowing treatment and species, and varies from 6094%. Time for germination varies from 3045 days. Soaking annona seeds in gibberellic acid solution is recommended, but not essential, to improve germination, at concentrations from 500 to 10,000 ppm for 12 to 72 hours, depending on the species. The higher the concentration, the lower the soaking time. Cover seeds in solution with a paper towel to keep them totally immersed. Cherimoya seeds can be soaked for 4872 hours in distilled water, or for a shorter time if heated to 92C and gradually cooled. Scarification techniques (cutting or scratching the seed coat) only slightly influences seed germination, and can lead to fungal attack and loss of germination capacity.

Sow seeds either in seedbeds or pots. Prepare raised seedbeds 1.2 m by 4 m, above soil level to ensure good drainage. Spacing: 13 cm between seeds and 10 cm between rows. For pots, sow 2 seeds per pot about 12 cm deep, then cover with a fine soil layer. Compact gently and water to saturation. Water once a day during the dry season and every 23 days during the wet season if necessary, to keep the seed-bed moist but not saturated. Remove weeds as they appear. Monitor pests and diseases daily, and control when numbers are low and use a minimum of pesticide. Ensure there is free movement of air to reduce damping off disease. Foliar fertilizer containing 5 g of urea and 15 g triple superphosphate per litre of water should start on 90-day-old seedlings. Repeat spraying monthly, before and after grafting, until planting out in the field. Keep seedlings in a nursery until their stems reach approximately 1 cm in diameter, when they can be grafted.

5.2.2 Vegetative Propagation

See Technical Note 4a in Part II. Vegetative propagation has many advantages over seed propagation; these are: The offspring will be genetically identical to the selected mother plant. Convenience and ease of propagation, selection and maintenance of clones. Shortening the time to start bearing fruit.

Budding and grafting are the two most commonly recommended methods of vegetative propagation. Both methods take a high quality or desirable scion and attach it to a compatible seedling rootstock. Carry out budding and grafting in the spring, or the periods in equatorial climates corresponding with the start of sap flow. The exception is sugar apple, for which budding is recommended prior to leaf abscission, during the dry season. Use a sharp knife and polythene tape (1.5 cm wide, 25 cm long).

5.2.3 Budding Method

See Technical Note 4b in Part II. Budding methods can be 80% successful. Sugar apple is best propagated by budding. Take a scion (bud) from a superior annona tree with high yield and fruit quality. Use shield or T budding to insert the bud into the stem of a 1015-month-old compatible rootstock with a diameter equal to the piece of bark that was removed. Tie the bud firmly with the polythene tape to keep it in position. Remove the polythene tape after 1 month, allowing the bud to grow into a new shoot. Tie the new shoot to the rootstock stem to keep it in an upright position. The rootstock should be cut when the leaves of new shoots are totally expanded. The newly budded tree can be planted 6 months after the budding operation or when 4 6 leaves have been produced.

5.2.4 Grafting Method

See Technical Note 4b in Part II. Many annona trees have shown a higher percentage take when propagated by grafting rather than by other methods. Splice grafting is one of the most important grafting methods for annonas and has given a success rate of up to 90%. The method for splice grafting is as follows: Graft annona scions onto preferred rootstocks 15 months after transplanting from the seedbed, when the rootstocks are 3040 cm tall and the stem diameter is 0.81.0 cm. The seedling rootstock should have a diameter similar to that of the scion taken from a superior cultivar (1 cm). Cut the seedling rootstock horizontally, approximately 20 cm above the level of the soil, with the sharp knife. Make a transversal cut (34 cm) in the rootstock stem. The mature shoot scion (graft-wood) from the selected cultivar should be 1720 cm long and 1 cm in diameter. The base of the scion should at a similar angle to the rootstock, so that the scion and rootstock can be joined together. Bind the scion/rootstock union firmly with grafting tape and cover with a plastic bag to maintain humidity. Tie off the grafting tape and thin plastic bag 45 cm below the grafting point. A new grafted plant will be ready for transplanting approximately 6 months later.

NOTE: TO INCREASE PERCENTAGE TAKE, REMOVE LEAVES FROM THE GRAFT-WOOD ONE WEEK BEFORE GRAFTING

5.2.5 Other methods

Stem cuttings Annona trees can also be propagated vegetatively by using stem cuttings. Orchards developed in this way can be very uniform, but rooting success is not always high: in custard apple it can be as low as 2%. Soursop cuttings do not produce a taproot and the resulting plants are susceptible to falling in high winds. To improve rooting, lengthen stems for 15 days in a mist propagator or by treating with naphthalene acetic acid (NAA) at 5000 ppm.

Root suckers Wild soursop, which has few known varieties, can be propagated by separating root suckers of desirable trees.

5.3 Nursery Establishment

See Technical Note 3b in Part II. After selecting trees for rootstocks and scions, establish the nursery so that an adequate environment can be created for young trees to grow, so that they establish well in the orchard.

5.3.1 Type and Size of Nursery

The type and size of nursery depends on the number of trees required for the orchard, and also the objectives and financial resources available to the grower. When the cost of grafted trees is low and the farmer is uncertain about grafting techniques, it is better to purchase the plant material instead of building nurseries and producing their own stock. If grafted trees are not readily available, subsistence farmers in smallholder conditions requiring only a few trees, can use a cheap temporary nursery. This can be under a shady tree. Use posts of durable wood as support for shade. Use young leaves of coconut palms or grass for shade to protect the seedlings. Training in grafting techniques should preferably be made available by the horticultural extension office. For growers aiming to produce grafted plants for sale, an improved and longer-term nursery should be established. This nursery should have an appropriate shade netting roof allowing 70% sunlight penetration. Grass or palm fronds may also be used, but will need replacing from time to time. Both types of nurseries should be situated close to a water source and must be protected by a fence against animals such as goats, chickens and cattle, which can easily destroy or damage plants and structures.

Example calculation for the establishment of custard apple rootstock trees to be grafted with soursop scions: A 5 ha soursop orchard with spacing of 7 x 7 m (204 trees/ha), needs 1020 grafted trees. Sow 55% more seeds than calculated, to produce the number of grafted plants needed. Good seeds for rootstock establishment have a germination success of about 70%. Bud or graft take is about 80% successful, and field losses after transplanting are about 5%. Therefore, the number of seeds sown should be 1581 (1020 plus 561 seeds). When rounding off, always round up. For sowing 1600 seeds (sown directly into 20 cm diameter plastic nursery bags), a nursery should have a covered area of 110 m2.

5.3.2 Pots and Seedling Media

See Technical Note 3a in Part II. Black plastic bags (approximately 22 cm diameter, 25 cm length) with holes near the bottom are the most suitable pots used for direct sowing and growth of annona seedlings. They are also relatively cheap. Different types and sizes of pots can be made from locally available materials, such as clay, tin cans (punctured) or woven baskets.

When mixing seedling medium, be aware of the following: Seedling medium should be composed of fine river sand, or a mixture of two parts of fine sand and one part of top soil. To avoid nematodes and fungal attack on young seedling roots, use a cheap and efficient technique called solarization, for soil sterilization. This requires a seedling bed with a low wall 30 cm in height, made of wood or brick and cement, about 2 m wide and 10 m long, depending on the amount of seedling medium needed. Put seedling medium in this structure and cover with transparent plastic for a minimum of 3 days of full sun. The action of sunlight increases the soil temperature to above 50C which kills most infectious soil micro-organisms.

5.4 Field Establishment

5.4.1 Site Preparation
See Technical Note 5a in Part II. Clear the land of shrubs and weeds. For small scale plantings Mark out tree positions with stakes. Dig holes as detailed in 5.4.5 For larger scale plantings If practicable take a sample of soil for analysis 46 months before planting. Take note of nutrient deficiencies in existing plants by looking at leaf colour particularly, and seek advice from soil lab or extension department to rectify any deficiencies by application of fertilisers when planting Put in either contour or subsurface drains if they are needed. Plough to a soil depth of 30 cm and harrow twice, 1 to 2 months before the wet season, to eliminate weeds. On slopes greater than 3%, prepare the ground with contour ploughing to avoid soil erosion. Plant in a triangular system, starting in the upper contour. Flat land with slopes lower than 3% do not require contour ploughing and a rectangular or square system of planting can be used.

5.4.2 Time of Planting

Plant at the beginning of the wet season, to minimize watering after planting, especially if there are seasonal dry periods and no irrigation facilities. If irrigation is provided, the time of planting is unimportant, although low relative air humidity can cause leaves to become dry.

5.4.3 Direct Seeding into the Field

Direct seeding can save time and money by avoiding nursery management of the rootstock, but losses are very high and it is not recommended. 8

Grafting seedlings in the field is not often very successful.

5.4.4 Windbreaks
Annonas have shallow rooting systems, leaving them susceptible to wind damage. In exposed areas, establish windbreaks prior to transplanting into the field. Casuarina and Glyricidia can be used as windbreaks. Other species may prove useful locally.

5.4.5 Transplanting and Spacing

In general, annona plants should be produced in a nursery and are ready for transplanting into the field or for use as rootstocks when they are about 10 months old. The ideal time for transplanting is when plants are 3045 cm high or when 4 to 6 leaves have matured. A graft or bud union should be about 16 cm above ground level when transplanted. For planting in adverse areas such as wastelands, plants may be up to 1 years old. Before transplanting, prune the leaves in half to reduce transpiration. Ensure the roots are not coiled up in the planting bag. Straighten out if necessary. Cut the tip of the main root to induce production of more lateral roots. Dig a pit about 4560 cm deep and fill with a mixture of soil and 1015 kg farmyard manure; wider pits might be needed on poorer soils. Ensure that the soil surface of the nursery bag in which the grafted plant developed is level with the ground surface. Construct a small basin around the tree and water the young plants as soon as possible after transplanting Tie plants to wooden stakes to avoid wind damage. Mulch to reduce soil drying around the newly transplanted materials.

Spacing depends upon the Annona species: Cherimoya 8 m x 6 m to 6 m x 4 m Soursop 8 m x 8 m to 4 m x 4 m Sugar apple 5 m x 5 m to 3 m x 3 m Custard apple 8 m x 8 m to 6 m x 6 m Wild soursop 5 m x 5 m Grafted and budded plants generally need the closer spacing, and seedling plants the wider spacing, as given above. The distance for planting on poorer soil can be 4 m apart, e.g. rain-fed wastelands.

5.5 Field Management

See Technical Note 5b in Part II. Careful orchard management is necessary to ensure high fruit yields of good quality. These operations are described below.

5.5.1 Weeding
In small annona orchards, eliminate weeds by using tools such as hoes or mattocks. Mulch during the first years after planting; to reduce weed infestation, reduce evaporation losses from soil, and avoid crust formation of the soil. Dried grass or husks of rice can be used for mulching. In an irrigated orchard mulch may be used only during the first year after transplanting. Herbicide spraying with paraquat or glyphosate can be considered when reduction of the cost of hand labour for orchard maintenance is involved. 9

Do not spray under windy conditions as spray drift can harm the trees.

NOTE: WHEN MULCHING WITH DRIED PLANT MATERIALS TO PROTECT SOIL EROSION, BE VIGILANT AGAINST FIRE DURING THE DRY SEASON.

5.5.2 Irrigation
Young trees require water in the nursery and during establishment in the field. The need for water depends on the species, soil type and local climate. Adult annona trees may grow and produce poorly without irrigation. When planting in the field at the beginning of the rainy season, ensure the trees are watered if dry spells occur so that the soil does not dry out, but be careful not to saturate soil. The first dry season is the most critical period when the young tree can die for lack of water before the roots become well established. Apply 7 10 l water to the basin every 10 15 days. If the leaves hang down and appear wilted, increase the amount of water at each irrigation, or reduce the number of days between each irrigation. During the second wet season no irrigation is likely to be needed unless a dry period occurs. In the second and third dry seasons apply 12 15 l water to the basins every 12 15 days depending on the temperature, winds and soil type. Watch the trees carefully for signs of water stress and apply water as needed.

The best time to apply water is in the early morning or late afternoon. Try to avoid midday as this can stress the tree. For small scale plantings: Construct a basin around the tree and fill with water every 7-10 days during the dry season. Or, two unglazed 5 litre earthenware pots sunk into the ground near the tree filled with water may be used.

For larger scale plantings: The aim is to ensure the trees do not become stressed through lack of water during the flowering and fruiting period. During the dry season irrigation should be withheld for 8-10 weeks to ensure dormancy and flower bud initiation. If plenty of water is available from a borehole, well or stream, furrow or basin irrigation can be considered. This is one of the cheapest methods, but uses more water. If water is in short supply, drag line or micro-sprinkler irrigation can be used although it costs more to install. Two micro-sprinklers per tree are preferred, with 180 of water distribution. The micro-sprinklers should not moisten the tree trunk, to avoid fungal or bacterial diseases. An adult bearing annona tree requires approximately 150 litres of water every two days (2 microsprinklers of 25 litres/h each, for 3 hours) during the dry season.

Drip irrigation methods are also suitable. Sugar apple is often a rain-fed crop. The fruits benefit from rainfall received during the fruiting season but one or two irrigations can be beneficial and bring on early bearing. Wild soursop grows in areas of relative humidity (RH) as low as 66% at noon, but requires 6001200 mm annual rainfall. Neither sugar apple nor wild soursop can tolerate waterlogging.

10

5.5.3 Fertilization
Fertilizer rates vary among annona species and with tree age. Soil analysis is highly recommended as a guide to correct fertilizer applications. See Appendix IV for examples. For small-scale plantings: Regular applications of inorganic fertilizers may be too costly for small scale producers. In the absence of fertilizers, apply compost or farm yard manure at 1012 kg per tree at the beginning of the rainy season. If yellowing of leaves becomes evident during the wet season, apply 250 g nitrogen as a top dressing. Smallholders may also use N:P:K at 250:125:125 g per tree per year or locally available materials such as bonemeal and castorcake. For larger scale plantings: In the absence of soil analysis, the following guidelines are given, but these may vary form location to location. Consult the local research station or extension department to obtain a recommendation. Farmyard manure (510 kg), lime (200300 g) and phosphorus (800 g of triple superphosphate) are commonly used in the planting holes of annona trees. Note: lime may be omitted for sugar apple and wild soursop. Ninety days after planting, apply 100 g of ammonium sulphate and 60 g of potassium chloride as a top dressing to all annona trees. Adult trees (bearing plants) should be fertilized with 3 kg of ammonium sulphate, 660 g of triple superphosphate and 500 g of potassium chloride, applied in three equal portions during the year, preferably at the beginning of, in the middle of, and late in the rainy season. The fertilizer should be lightly incorporated into the soils around the tree. Wild soursop is known to respond to occasional application of magnesium fertilizer. Fertilization by foliar spraying is potentially important to supplement short-term nutrient requirements for annona trees, especially for micronutrients. Apply boron at 2.0 g/m2 and broadcast over the ground area under the canopy. Boron and calcium sprays during flowering and early fruit set are beneficial in reducing internal fruit browning. Spraying of 0.1% zinc sulphate, at monthly intervals, will correct zinc deficiency, identified by yellowing of leaves between the veins.

5.5.4 Pruning and Training

Pruning and training influence growth by regulating the balance between vegetative and fruiting phases. There are two types: Pruning for vegetative growth and shape. Pruning for fruit production. (This system involves chemical leaf removal and is not recommended for smallholder annona production.)

Pruning for vegetative growth and shape Pruning for vegetative growth and shape has several objectives: Development of good tree architecture to support future yield. Good aeration and light penetration of canopy. Ease of application of cultural practices, e.g. hand pollination, pesticide spraying and harvesting. Removal of lower limbs touching the ground and branches rubbing against each other.

This type of pruning should start in the first year and continue until the fourth year after planting out, but this depends on the annona species. 11

Training cherimoya trees should begin in the nursery. Sugar apple and soursop trees do not require much training. Sugar apple budded plants are very lightly pruned about every 2 years, but in arid regions do not require any pruning.

How to prune First year, in the spring, cut the single trunk to 80 cm above the ground, to stimulate production of primary branches. Second year, in the spring, cut the primary branches to a length of 40 cm, to stimulate production of secondary branches. Third and fourth years, prune the secondary and tertiary branches, at 30 cm and 20 cm lengths respectively. The new branches should be spaced 1525 cm above each other in different directions to develop a good scaffold by the fourth year after planting. At this age, the plant is about 2 m in height, after which it can be left to grow naturally.

5.5.5 Intercropping and Cover-cropping

Growers can intercrop annonas with annual or vegetable crops to get additional income, to compensate for labour and other costs in the orchard during the first 23 years before first harvest. Intercropped legumes can be planted for the first 56 years. Plant perennial leguminous or grass cover-crops to avoid soil erosion and to improve soil physical structure.

NOTE: INTERCROPPING WITH CLIMBING ANNUAL SPECIES, SUCH AS CLIMBING BEANS, MAY BE HARMFUL IF THE CREEPING LEGUME COVERS THE ANNONA TREE AND REDUCES AVAILABLE LIGHT. INTERCROPS AND COVER-CROPS ALSO REQUIRE MANAGEMENT.

5.5.6 Pollination and Fruit Set

Natural pollination of annona species is relatively ineffective, especially outside their natural range where insects rarely visit their flowers. Exceptions to this are some Nitidulid beetles. Three Nitidulid beetles per flower increase fruit set to nearly 25%. These insects breed rapidly in rotting fruit and their numbers can be increased by using a rotting-fruit attractant. Fertilization failure of all or several ovules results in small or asymmetrical fruits, which affects marketing. Hand pollination is necessary to ensure commercial production of annonas in most places. It guarantees significantly higher yields and better fruit quality than insect pollination. Pollination carried out in the morning is better than in the afternoon, and cross-pollination is to be preferred to self-pollination. Fruit set of most annonas is very low in warm temperatures.

The hand-pollination technique has the following steps: Collect mature donor flowers (source of pollen) in the afternoon from the terminal portion of the branch and place them in a small paper bag. Keep this paper bag in a cool and dry environment. In the morning of the next day, remove the petals and put all the stamens into a cylindrical plastic container (a film canister, for instance) to facilitate carrying. Select flowers that have already started opening, and remove their petals. Apply the pollen onto the pistils using a brush, with back and forth strokes.

12

The stigmas are only receptive for a short time and not when the pollen is shed. Collection of pollen is thus essential the day before the stigmas become receptive. Some trees shed pollen in the morning and others in the afternoon; watch for this to aid collection. NOTE: COLLECT FLOWERS FOR HAND POLLINATION FROM THE TERMINAL PORTION OF BRANCHES, SINCE THEY GENERALLY PRESENT LOW FRUIT SET ANYWAY.

Fruit thinning is necessary to regulate crop load and to maintain fruit size. The thinning operation involves removal of misshapen fruit and the reduction of fruit clusters to improve fruit quality and size.

5.5.7 Pests and Diseases

See Technical Note 5b in Part II. Annona trees are subjected to attack by a large number of insect pests and diseases. Only major and minor pests and diseases of economic importance and their possible methods of control are described here and in Appendices 2 and 3. More detail is presented in the companion monograph. Monitor the pest and disease status of the orchard continually to prevent an epidemic attack from developing. Biological control methods are preferred and should be practised within an integrated pest management strategy. Serious pest or disease attacks should be reported to a local Extension Officer for recommendations about control methods, pesticide application rates, and their level of toxicity. Do not spray fruit within 21 days of harvest to avoid toxic deposits on the fruit skin. Spraying should be avoided if possible, especially during fruit bearing, or if the crop is near dwellings, and if there are strong prevailing winds. Empty pesticide containers should be discarded only according to government regulations.

Most important annona pests The trunk borer (a Coleoptera beetle). The fruit borer (a Lepidoptera moth). The seed borer or wasp. Fruit-flies are important pests attacking most annonas, except for soursop.

Other pests Control The removal of affected branches (for trunk borer) or attacked fruit (for moth and wasp attacks) is the first recommended method of control. Use a plastic bottle as a fruit-fly trap to monitor the infestation level of fruit-flies in the orchard. o Make four or five small windows (4 x 5 cm) around the middle part of the bottle to allow insect entrance. Mealy bugs, various species of scales or cochineals, and spider mites cause damage on plants and fruits. Aphids, fruit-spotting bugs and other hemipterous bugs (stink bugs), leaf hoppers, leaf miner larvae, root grubs and ants can damage annona trees or fruits, making them unmarketable. Sucking insect pests may be important economically, when they attack young growing fruits.

13

o o o o

Place 100 ml of an attractant poison mixture inside the bottle. A stock of this mixture can be prepared with water (10 litres), sugar cane syrup (0.7 kg) and the insecticide fenthion (10 ml). Use 4 bottles for a one-hectare orchard or 2 bottles per hectare for orchards between 2 and 5 hectares in area. When one fruit-fly is found inside one or more bottles start fruit-fly control in the orchard. Control by applying the attractrant poison mixture in 1 m2 of canopy of trees, in a ring form around those trees where fruit-flies were found in the bottles. Wet a paintbrush with the mixture, then spray the mixture onto the canopy with a flicking motion of the brush.

Diseases Several diseases attack either seedlings in the nursery or adult annona trees and their fruits in the field and post-harvest. See Appendix 3A for the major diseases common names, causal agents and their controls. Major annona diseases Damping-off, black root rot, bacterial wilt, anthracnose, black canker, diplodia rot, purple blotch, brown rot and fruit rots. Ensure good ventilation and free draining soil medium in the nursery to reduce damping-off and other seedling diseases. For fruiting trees, remove infected fruits and spray with copper oxychloride 0.2% or benomyl at 0.1%.

Minor diseases Burning string, zoned spot, blight, black scab, fumagina, rubelose, cercosporiose, armillaria root rot and nematodes. Normally these diseases do not justify the cost of spraying. If possible remove infected plant parts and burn to reduce the spread of disease.

6. HOW TO HARVEST ANNONA TREES

6.1 Time to First Harvesting
See Technical Note 6a in Part II. Annona species usually start flowering between 36 years after planting out, depending on the type of propagation, cultural practices such as pruning or hand pollination, and climate. Flowering occurs gradually over many months, so harvest time is spread over a period of months also.

6.2 Ripeness and Yield

Annona fruits reach complete ripeness individually, so harvesting should be carried out selectively. The time to harvest is called the harvest point, determined by fruit skin colour, which changes during the transition from physiological maturity to full eating ripeness. Cherimoya and sugar apple change from greyish green to yellowish-green, but their pulp should be firm. Soursop skin changes from dark green to slightly yellowish-green. Sugar apple fruits reach the harvest point for local markets when the segments (carpels) are spread far apart, exposing a yellow creamy skin in the inner part between carpels. 14

Wild soursop is ripe when the white specks on the skin become orange-yellow.

Skin colour index A skin colour index to guide harvest depends on the market place. For local market, harvest annona fruits with 20 to 40% yellowish skin, which will ripen between 4 to 6 days later. For sugar apple, there is a very short time (maximum 3 days) from harvest point to consumption point. For export markets 10% to 20% yellow is satisfactory. Fruits harvested with more than 75% yellowish skin ripen within 3 days. Those harvested with less than 5% do not ripen completely. The most suitable time of day to harvest is after overnight dew has evaporated, when fruits are dry and fungal rot contamination is less likely.

The yield of an annona tree varies depending on region, species or cultivar, cultural practices and climatic conditions. Cherimoya cv. Fino de Jete, at 7 years old, yields an average of 43 kg of fruit per tree. Soursop, beginning to bear when 3 years old, yields 24 fruits per tree, and when 7 years old reaches its maximum yield of 100 kg per tree. Sugar apple, 6 years old, can yield 5060 fruits per tree. In semi-arid tropical climates an irrigated sugar apple tree can produce two harvests: a main harvest during the rainy season, with a higher yield (60 fruits per tree), with a second harvest with a lower yield occurring during the dry season (< 30 fruits per tree). Custard apple can produce up to 150 fruits per tree.

6.3 Harvesting Techniques

Annona fruits should be hand-harvested by cutting the stalk with pruning scissors, leaving 0.5 to 1 cm of it to avoid loss in weight and post-harvest fungal diseases. Depending on tree size some species, such as sugar apple or soursop, are harvested by climbing the tree, using a ladder or a picking pole with a hook and a basket at its end. Soursop harvest is more difficult and time-consuming than other annonas because trees are usually taller and fruits are larger. Soursop left on the tree will eventually fall off naturally and, on the ground, will become rotten and unmarketable. These fruits should be picked up and destroyed as they encourage pests and diseases to reproduce and spread throughout the orchard. To keep the orchard in good condition all annona fruits should be harvested.

7. POST-HARVEST HANDLING AND PROCESSING

7.1 Post-harvest Handling
See Technical Note 6b in Part II. After clipping fruit from the tree: Put the fruit into cushioned boxes or baskets to avoid mechanical damage or bruising. Keep the boxes in the shade and protect against rain, wind and dust. Since annona are considered climacteric fruits, storage temperature should be well controlled after harvesting. Tropical annona fruits, such as soursop and sugar apple, require higher storage temperature than subtropical cherimoya fruits.

15

Inspect the fruits daily to evaluate their state of ripeness. Custard apple and wild soursop have very short shelf-lives, and treatments to extend them are too costly. It is possible to pick slightly under-ripe fruit, but even then keeping time is probably only about 4 days.

After harvesting, annonas may be stored on racks if ambient temperatures are suitable or cooling facilities are available. Cherimoya should be stored at about 1112C and will remain in good condition for 21 days. Sugar apple should be stored at 1516C and relative humidity of 85%. At this temperature, 7 days of storage is possible with minimum change in fruit quality. Soursop is stored at 20C in the shade.

Post-harvest control of fungi to increase storage life: Immerse in fungicide (thiabendazole 8g/l) and keep in polythene packaging with ethylene absorber. Ethylene absorber is made from one 6 cm3 clay brick, wet with 8 ml of aqueous solution of potassium permanganate. After storage, fruits are ready for pulp extraction or can be sold for fresh consumption.

7.2 Processing
See Technical Note 7a in Part II. Most annonas, except soursop, are sold as fresh fruits, although they can be processed as juice, frozen pulp, jelly and ice cream. Cherimoya and sugar apple are mainly consumed as dessert fruits. However, mixing pulp with milk results in a delicious drink or ice cream when frozen. Annona pulp is sweet, with moderate acidity, and its texture and flavour are excellent. Soursop is more suitable for processing because its pulp does not oxidize as easily as sugar apple and cherimoya.

Soursop concentrate is processed by following these steps: Fruit should be selected, rinsed, and hand peeled, and the seeds removed. Heat pulp to 80C for 1 minute then cool and determine soluble solids content (a measure of the sugars), using a refractometer or hydrometer. Add 10 g of sodium benzoate per 10 kg of pulp, blend for 10 minutes, then sieve. Add sugar and eliminate the air inside the pulp by compression, then concentrate it at 100oC for a few minutes. Place pulp into a storage container, cover, then cool, label and store it. Soursop should be hand-peeled and cored, as fruits have fragile skin, irregular shape and soft pulp, all of which limit machine processing. Soursop pulp processed below 93oC and frozen into polythene bags offers a high quality product with no loss of taste or smell. After processing, the pulp, sweetened or not, can be transformed into numerous other products. Puree can be used to prepare ice cream, drinks, sherbets and gelatin. To obtain nectar for juices, marmalade and jams, mix in 10 litres of water, 1.7 kg of pulp, 1 kg of sugar, 2 g of sodium benzoate and 2 g of sodium metabisulphite. This mixture should be heated at 100oC for 15 minutes.

Oxidation is a very common problem with processed annona pulp. To avoid oxidation, the pulp can be heated at 70oC for 20 minutes, and 0.5% of ascorbic acid can then be added. This preparation can be stored in polythene bags for one month at 5oC in a refrigerator. Frozen pulp should be kept at -18C. 16

8. MARKETING
8.1 Marketing Potential
See Technical Note 7b in Part II. Marketing involves the processes from the time the fruit is picked to when it reaches its final destination. This may be the consumer for fresh fruit, or through the different stages of processing to an end user. Marketing is often the weakest link in the production to consumption chain and one in which annona producers must participate in order to sell their products. Annona fruits are mainly traded within the country of production in local or urban markets, or sent to factories for processing. Cherimoya has well-established international marketing channels, with Spain and Chile as the main producers and exporters.

8.1.1 Constraints to international markets

Annona production suffers from insufficient financial support for research, transport, roads, extension services or farmers organisations. There are high levels of post-harvest losses due to short shelf life. Processed pulp may be of low quality and fail to meet international standards. There is a lack of international market information.

8.1.2 Expected returns

Crop Cherimoya Fino de Jete Brazil (7 yr old) Sugar apple (6 yr old) Soursop (7 yr old) (see Pinto et al. 2005) Year Yield 33kg/tree (417 trees/ha) 60 fruits/tree 2000 fruits/ha 100kg/tree Price Price US$ 1.8/kg Income US$ 24,800 US$ 10,000/ha US$ 48,165

Establishment/Maintenance (US$)

Average yield (tonnes fruit/ha) 2 4 5 7 9 10

Gross income (US$) 0 1,360 2,720 3,400 4,760 6,120 6,800

Net income (US$) 0 644 2,045 2,477 3,764 4,936 5,588

1st 2nd 3rd 4th 5th 6th 7th

2,486 716 675 923 996 1,184 1,212

(see Pinto et al. 2005) Based on 204 plants/ha, and estimated gross and net incomes. A great advantage of soursop is that value can easily be added to its production, since it can readily be sold as a fresh or a processed fruit. It is very important that small annona farmers consider implementing their own processing to add value to their raw product in order to increase income. 17

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(1998) Improving Fruit Set of Cherimoya (Annona cherimola Mill.) cv. Cumbe, by Autogamous and Allogamous Hand Pollination. Proceedings of the Interamerican Society for Tropical Horticulture, 41 : 162-165. FAO (1988) Traditional Food Plants - A Resource Book for Promoting the Exploitation and Consumption of Food Plants in Arid, Semi-Arid and Sub-Humid Lands of Eastern Africa. FAO Food and Nutrition Paper, 42. Freire F. de C. O. and Cardoso J. E. (1997) Doenas das Anonceas. [Portuguese] In: Proceedings of I Brazilian Symposium on Annonceous. Edited by A. R. So Jos, Souza I. V. B., Morais O. M. and Rebouas T. N. H. Universidade Estadual do Sudoeste da Bahia, Vitria da Conquista, Bahia, Brasil : pp. 196-213. Fuentes L. J. (1999) Production of Cherimoya (Annona cherimola Mill.) in Ecuador. In: Proceedings of the First International Symposium on Cherimoya. Edited by V. Van Damme, Van Damme P. and Scheldeman X. ISHS, Loja, Ecuador. Acta Horticulturae, 497 : 59-63. George A. P. and Nissen R. 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Universad de Ciencias y Artes del Estado de Chiapas, Tuxtla Gutirrez, Chiapas, Mxico : pp. 81-82. Herrera A. L. (1999) Biodiversidad, Bioseguridad y Propriedad Intelectual de los Recursos Geneticos. [Spanish] In: Memrias del II Congresso Internacional de Anonceas. Universad de Ciencias y Artes del Estado de Chiapas, Tuxtla Gutirrez, Chiapas, Mxico : pp. 31-42.

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INIA (1997) El Cultivo del Chirimoyo. Instituto Nacional de Investigacin Agraria. Boletim Tcnico 11, Lima, Peru. : 28 pp. Lahoz J. M., Gutierrez M., Sola M. M., Salto R., Pascual L., Martinez-Cayuela M. and Vargas A. M. (1993) Ethylene in Cherimoya Fruit (Annona cherimola Mill.) Under Different Storage Conditions. Journal of Agricultural Food Chemistry, 41 (5) : 721-23. Leal F. (1990) Sugar Apple. In: Fruits of Tropical and Subtropical Origin Composition, Properties and Uses. Edited by S. Nagy, Shaw P. E. and Wardowski W. F. Florida Science Source, Inc., Lake Alfred, Fla. : pp. 149-158. Lizana L. A. and Reginato G. (1990) Cherimoya In: Fruits of Tropical and Subtropical Origin: Composition, Properties and Uses. Edited by S. Nagy, Shaw P. E. and Wardowski W. F. Florida Science Source, Lake Alfred, Florida, USA : pp. 131-148. Moreno Andrade R., Luna Cazres L. and Gonzlez Esquinca A. R. (1999) Estudios Sobre la Germinacin de Annona lutescens. [Spanish] In: Memorias del II Congreso Internacional de Anonceas. Universad de Ciencias y Artes del Estado de Chiapas, Tuxtla Gutirrez, Chiapas, Mxico : 82 pp. Moror R. C., Freire E. S. and Sacramento C. K. (1997) Processamento da Graviola para Obteno de Polpa. [Portuguese] In: Anonceas: Poduoo e Mercado (Pinha, Graviola, Atemia e Cherimlia). Edited by A. R. So Jos Boas I. V., Morais O. M. and Rebouas T. N. H. Universidade Estadula do Sudoeste da Bahia, Vitria da Conquista, Brasil : pp. 263-274. Nakasone H. Y. and Paull R. E. (1998) Annonas. In: Tropical Fruits. Edited by H. Y. Nakasone and Paull R. E. CAB International, London,UK. : pp. 45-75. Pareek O. P. (1985) Fruit Crops. In: Efficient Management of Dryland Crops. Research Institute for Dryland Agriculature, Hyderbad, India. : pp. 293-408. Pinto A. C. de Q., Cordeiro, M. C. R., de Andrade, S. R. M., Ferreira, F. R., de C. Filgueiras, H. A., Alves, R. E. and Kinpara, D. I. (2005) Annona species, Internaitonal Centre for Underutlised Crops, University of Southampton, Southampton, UK. Pinto A. C. de Q. and Ramos V. H. V. (1997 a) Melhoramento Gentico da Graviola. [Portuguese] In: Proceedings of the I Brazilian Symposium on Annonaceous. Edited by A. R. So Jos, Souza I. V. B., Morais O. M. and Rebouas T. N. H. UESB, Vitria da Conquista, Brazil : pp.55-60. Pinto A. C. de Q. and Ramos V. H. V. (1997 b) Graviola: Formao do Pomar e Tratos Culturais. Anonceas, Produo e Mercado. In: Anonceas, Produo e Mercado. [Portuguese] Edited by A. R. So Jos, Souza I. V. B., Morais O. M. and Rebouas T. N. H. Universidade Estadual do Sudoeste da Bahia, Vitria da Conquista, Bahia, Brasil : pp. 94-104. Pinto A. C. de Q. and Silva E. M. (1994) Graviola para Exportao: Aspectos Tcnico da Produo [Portuguese] FRUPEX, Min. Agricultura, do Abastecimento e da Reforma Agrria, Sec. de Desenvolvimento Rural SDR, Prog. De Apoio Prod. E Export. De Frutas, Hortalias, Flores e Plantas Ornamentais. : 41 pp. Pinto E. T. (1991) Recomendaciones Tecnicas para el Cultivo del Guanabano en las Region Altantica de Costa Rica. [Portuguese] Ministerio da Agricultura y Ganadeira, Siquirres. : 22 pp. Popenoe W. (1974 a) The Anonaceous Fruits; The Cherimoya. In: Manual of Tropical and Subtropical Fruits, Facsmile of the 1920 ed. Hafner Press, a Division of Macmillan Publishing Co, Inc., New York, Collier-Macmillan Publishers, London, Chapter 5 : 161-189. Popenoe W. (1974 b) The Anonaceous Fruits; The Soursop. In: Manual of Tropical and Subtropical Fruits, Facsmile of the 1920 ed. Hafner Press, a Division of Macmillan Publishing Co, Inc., New York, Collier-Macmillan Publishers, London, Chapter 5 : 182-186. Purohit A. (1995) Annonaceous Fruits. In: Handbook of Fruit Science and Technology: Production, Composition, Storage and Processing. Edited by D. K. Salunkle and Kadam S. S. M. Dekker, New York, USA : pp. 377-385. Rao V. G., Desai M. K. and Kulkarni N. B. (1962) A New Phytophthora Fruit Rot of Annona senegalensis from India. Plant Disease Reporter, 46 : 874-876. Rungsimanop C., Suksri A. and Srinukul S. (1987) Some Irrigation Methods which Influence the Growth of Custard Apple and Papaya when Intercropped in Northeast Thailand. Phytochemistry, 30 (10) : 3335-3338. Sadhu M. K. and Ghosh S. K. (1976) Effects of Different Levels of Nitrogen, Phosphorus and Potassium on Growth, Flowering, Fruiting and Tissue Composition of Custard Apple (Annona squamosa L.). Indian Agricultural, 20 (4) : 297-301. Sanewski G. M. (1991) Custard apple - Cultivation and Crop Protection. Information Sries QI90031. Queensland Department of Primary Industry, Brisbane, Australia Santos C. R. (1997) Irrigao em Anonceas. [Portuguese] In: Proceedings of the I Brazilian Symposium on Annonaceous. Edited by A. R. So Jos, Souza I. V. B., Morais O. M. and Rebouas T. N. H. UESB, Vitria da Conquista, Bahia, Brazil : pp. 105-117. Tijero R. F. (1992) El Cultivo del Chirimoyo en el Peru. [Spanish] Ediciones Fundeagro, Lima, Peru. .108 pp. Yang C. S. (1998) Application of Plant Growth Regulators on Annona culture. In: The Application of Plant Growth Regulators on Horticultural Crops, Symposium Proceedings. Edited by H. S. Lin, Chang L. R. and Lin J. H. Changhua, Taiwan : pp. 305-320 (Special Publication, 12).

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APPENDIX I MULTIPLE USES OF ANNONA

TREE PART Fruit and pulp TYPE OF USE Fruit is sold in the market for fresh consumption of pulp. Pulp can be processed as frozen pulp, juice, jelly, sherbet, and ice cream. Pulp is often mixed with wine, milk (to make milk shakes) and yoghurt, processed into sherbet, baked into cookies and pastries, and made into fermented liquors. Unsweetened pulp can be processed to prepare nectars, soft drinks, ice creams and similar food. Essential oils are present in soursop pulp, such as esters of aliphatic acids, which have potential to improve the flavour of processed fruit products. Some flavonoids, alkaloids, tannins and saponins are effective as antispasmodics and relaxants, and as cathartic remedies, and are also used in the prevention of haemolysis of red blood cells. Resin is used for pupil dilation. Extract of smashed seeds releases alkaloids, flavonoids, tannins or saponins with good insecticidal activity (control of fruit flies). Acetogenins in seeds have shown good results in cancer treatments. Leaf extracts contain alkaloids, flavonoids, tannins or saponins with good insecticidal activity (control of mosquitoes). Leaves have alkaloids, such as murisolin, couxine, couclamine, stepharine and reticulin with antibacterial effect. Alkaloids and flavonoids in leaves have been used in trypanosomiasis treatment and also as antiulcerous and gastric treatment. Acetogenins of leaf extract have been tested as a treatment for cancer tumours. Used to stuff pillows. Some alkaloids, flavonoids, tannins and saponins of flowers have been indicated for use in eye inflammatory healing process. Extracts of some chemical compounds and alkaloids, such as murisolin, couxine, couclamine, stepharine and reticulin, have been used for treatment of skin diseases and for control of intestinal worms (vermifuge). Bark is used to produce a yellow-brown dye. Bark is used to make rope. Extract of alkaloids from root has shown purgative effect and has been used for treatment of male impotence, gastric processes and filariosis, as well as venereal disease. Acetogenins of root extract have been tested with acceptable treatment for cancer tumour.

Seed

Leaf

Flowers Twigs, shoots and bark

Root

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APPENDIX IIA MAJOR PESTS OF ANNONA AND THEIR CONTROL

COMMON NAME Trunk borer SCIENTIFIC NAME

Cratosomus b. bombina Helipus catragraphus Eurypages pennatus

NATURE OF ATTACK Larvae bore into tree trunk and branches causing sap exudation and tree death.

BIO-CONTROL Removal of affected parts; use of tolerant varieties.

Fruit borer (moth)

Cerconota annonella Heterographis bengalella

Larvae bore into fruit destroying its pulp completely.

Seed borer (wasp)

Talponia batesi Bephratelloides maculicollis Bephratelloides cubensis Anastrepha obliqua Ceratitis capitata Dacus dorsalis Batcrocera tryoni Planococcus spp., Dysmicoccus spp. Ferrisia virgata

Bores into fruit destroying the seeds completely. Larvae bore into fruit destroying its pulp completely.

Removal of attacked fruit from the ground; use of braconid insects (Apanteles spp.) to parasite larvae; bagging the fruits. Removal of attacked fruit from the ground; bagging the fruits. Removal of attacked fruit from the ground; bagging the fruits.

CHEMICAL CONTROL Painting trunk and branches with solution of 12 litres of water, 4 kg of quicklime, 100 g of sulfur, 1 kg of copper sulphate, 100 g of sodium chloride, 220 ml of diazinon, 600 ml of surfactant. Spray with fenthion 0.1% or thriclorphon 0.2% straight to young fruit, every 15 days until 15 days before harvest. Spray with decametrin 0.05% or thriclorphon 0.2% straight to young fruit, every 15 days until 15 days before harvest. The same control used for fruit borer moth (above). Spray solution of dimethoate at 0.05% plus mineral oil 0.5%.

Fruit flies

Mealy bugs

Scales Spider mites

Saissetia coffeae Ceroplastes spp. Pinnaspis spp. Oligonychus annonae Brevipalpus spp.

Sucks the sap of leaflets, mature and Removal of attacked twigs. tender shoots, and leaf petiole, causing leaf and leaf stalk chlorosis, causing dropping of young fruits. Spoils fruits by remaining in sunken areas. Sucks the sap of tender shoots. Removal of attacked parts in initial stages. Sucks and scratch adult leaves at dorsal part. No known bio-control.

Same control used for mealy bugs (above). Spray with propargite 0.15%.

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APPENDIX IIB MINOR PESTS OF ANNONA AND THEIR CONTROL

COMMON NAME Hoppers Moths Aphids Hemipterous Beetles Fruit-spotting bug Leaf larvae Leaf miners Wild bee (Irapu) Root grubs Ants SCIENTIFIC NAME

Empoasca fabae Membracis foliata Aethalion spp. Thecla ortignus Aphis gossipii Toxoptera aurantii Leptoglossus zonatus Antiteuchus tripterus Crimissa spp. Amblyphelta nitida Gonodonta nutrix Cocytius antaeus Pseudodirphia spp. Leucoptera spp. Phyllocnistis Trigona spinipes Anomala spp. Atta spp Acromyrmex spp.
spp.

NATURE OF ATTACK Sucks leaves and shoots transmitting some virosis. Larvae destroy leaves, flowers and shoots. Sucks sap of leaflets, young fruits and flowers causing the dropping. Leaflets become distorted. Sucks young fruit causing spots in its skin and further dropping. Destroy flower petals mainly from internal verticil. Sucks young fruit causing spots in its skin like canker. Destroy leaves and shoots. Mines leaflets and leaves causing the yellowing of them. Destroy leaflets and young flowers. Destroy tree roots. Destroy leaves completely.

BIO-CONTROL No known bio-control. Remove larvae by hand and kill them. Larvae of some Coccinelidae insects eat adult aphids. No known bio-control. Remove insects by hand in initial attack and kill them. No known bio-control.

CHEMICAL CONTROL Spray with malathion 0.2%. Spray with malathion 0.2%. Spray with malathion 0.2% when strong attacks occur. Spray on young fruits with decametrin 0.10%.

Spray with malathion 0.2% straight on flowers. Spray on young fruits with malathion 0.2%. Collection and destruction of Spray with trichlorphon 0.2% larvae by hand. or malathion 0.2% when attack occurs. No known bio-control. Spray with dimethoate 0.15% when attacks occur. Search and destroy the wild bee No effective control by nests. chemical spraying. No known bio-control. Application of aldicarb solution 0.2% around tree. Conical protector or chemical wet Organophosphate chemical to cotton around the trunk. control ants in the field.

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APPENDIX IIIA MAJOR DISEASES OF ANNONA AND THEIR CONTROL

COMMON NAME Damping-off Black root rot Bacterial wilt Anthracnose CAUSAL AGENT

Rhizoctonia solana, Phytophthora spp. Cylindrocladium clavatum Sclerotium rolfsii Pseudomonas solanacearum Colletotrichum gloeosporioides

BIO-CONTROL Treat potting media with solarization (see section 5.2 Nursery Establishment). Avoid excessive watering in seedbed; treat potting media with solarization. No known bio-control. Removal and burning of affected branches and fruits.

Black canker Diplodia rot Purple blotch Brown rot and fruit rots

Phomopsis spp.* Botryodiplodia theobromae Phytophthora palmivora Rhizopus stolonifer Glioclacium roseum

Cut and burn dried branches of internal part of canopy. Removal and burning of affected branches and fruits. Removal and burning of affected fruits. Same control for Phytophthora as indicated above.

* Phomopsis anonacearum often causes fruit rot of sugar apple in storage.

CHEMICAL CONTROL Watering of pots or seedbed with PCNB solution of 0.2%. Same treatment used for Damping-off (above). Spray with anti-bacterial chemical. Spray with copper oxychloride 0.2% intercalating with benomyl 0.1%, every 21 days until 21 days before harvest. Spray with Benolate 0.050 at 20-day intervals. Spray or paint trunk and branches with solution of benomyl 0.2% or copper oxychloride 0.5%. Spray with copper oxychloride 0.2% or paint the affected part with methyl tiophanate 1.2% plus surfactant. Same treatment used for anthracnose (above). No efficient treatment with chemicals is known.

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APPENDIX IIIB MINOR DISEASES OF ANNONA AND THEIR CONTROL

COMMON NAME Burning of string Zoned spot Black scab Black soot (Fumagina) Rust fungus Rubelose Cercosporiose Blight Armillaria root rot Nematodes CAUSAL AGENT BIO-CONTROL No known bio-control. No known bio-control. Avoid excessive wet condition to young seedling. Control of ants and aphids will help the control of fumagina. No known bio-control. CHEMICAL CONTROL Spray with copper oxychloride 0.15%, every fifteen days. When needed, spray with copper oxychloride 0.15%. Same control used for Damping off (Rhizoctonia solani, Appendix 3). Spray of mineral oil 0.5% then spray with copper oxychloride 0.2%. Same control used for disease Burning of string (above). Since these diseases have secondary importance, spray with copper oxychloride 0.15% when really needed.

Pellicularia koleroga Sclerotium coffeicolum Fusarium spp. Stigmella spp. Phakopsora cherimolae Corticum salmonicolor Cercospora annonae Phoma spp. Armillaria leuteobubalina Helicotylenchus spp. Meloidogyne spp.

Removal of affected tree parts. pH correction helps control. No known bio-control. Avoid excessive wet condition to young Same control used for Damping off (Rhizoctonia seedling. solani, Appendix 3). Rotation of planting and use of Aldicarb 10 g per tree. attractive plants like Crotalaria.

Source: http://www.hort.purdue.edu/newcrop/morton/ Cherimoya Soursop In Colombia this rate of fertiliser is used. In Hawaii this rate is used. At 6 months Apply N-P-K (10-8-6) lb (227 g) per tree. 6 months later: Apply N-P-K (10-8-6) 1 lb (454 g) Yr 2: N-P-K (6-10-8) 1 kg Yr 3: N-P-K (6-10-8) 1.5 kg Yr 4: N-P-K (6-10-8) 2 kg Yr 5 onwards N-P-K (6-10-8) 5 kg

APPENDIX IV - SUGGESTED FERTILISER RATES IN DIFFERENT COUNTRIES

Sugar apple In Egypt on sandy soils this rate is used. 60-80 kg manure per tree per year. N-P-K (3-10-10) 1.5 kg per year from year 4 onwards.

Yr1: Quarterly applications of N-P-K (10-10-10) 227 g per tree. Yr 2: N-P-K (10-10-10) 0.5 kg Yr 3: N-P-K (10-10-10) 1.5 kg Yr 4 onwards: N-P-K (10-10-10) 1.5 kg

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APPENDIX V - HEALTH AND SAFETY WHEN USING CHEMICALS

The following chemicals are examples of those that can be used for control of pests and diseases on annonas and the relevant health and safety advice for each. For a complete list of chemical controls and precautions for use, please check with your local extension or agricultural office. ALDICARB Active ingredients: carbamate (class) Handling and storage: Highly toxic through oral, skin contact and inhalation. Users should wear full protective clothing to avoid contact. Should be stored indoors in an isolated, well-ventilated, clean, dry, cool area. Store away from alkaline substances, water sources, food or animal feed. Environmental impact: Highly toxic to birds and other organisms. Aldicarb is persistent and may remain in soil and plant material over the entire growing cycle. Highly soluble in water and mobile in the soil. BENLATE Active ingredients: Benomyl Handling and storage: Users should wear protective clothing and avoid contact with the skin and eyes. The chemical may irritate the eyes, nose, throat and skin. Should be stored in an airtight container and kept away from water or fire. Environmental impact: Toxic to fish. DIMETHOATE Active ingredients: Dimethoate (organophosphate) Handling and storage: Protective clothing should be worn and washing facilities available. Environmental impact: Moderately toxic to humans and animals through ingestion, inhalation and through contact with the skin. It is also very toxic to birds, fish, bees and livestock. It is biodegradable and undergoes rapid degradation in the environment. It is highly soluble in water and is not toxic to plants.

25

GLOSSARY
Abscission Air-layering The process by which plant parts, such as leaves, are shed. A vegetative method of propagation in which a twig or shoot attached to the parent plant is wrapped in moist sphagnum moss and polyethylene plastic so that it will form roots, and can later be removed and planted. Period during which a flower opens or the act of flower opening. Seed produced by an unfertilized female cell or from somatic cells often in a way that mimics sexual reproduction. A vegetative method of propagation in which a bud from a parent plant is inserted in the stem of another plant (rootstock) to form a new plant. Fruits that show increased respiratory activity and release of ethylene during the ripening period, especially when stored in unsuitable temperatures. A system in which some crops (e.g. beans) are used to cover the ground and protect the main crop against erosion or to enrich the soil via nitrogen fixation. A vegetative method of propagation by which a portion of stem, root, or leaf is cut from the parent or stock plant and induced to form roots and shoots by chemical, mechanical, and/or environmental manipulation. A tree that sheds or loses foliages at the end of the growing season. The way in which someone causes the leaves of a tree to fall off by the use of chemical spray or dust. Male and female reproductive organs become receptive at different times within the same flower, to ensure cross- pollination. To blanch or whiten a plant part or a plant by excluding sunlight. The genetic makeup of an individual. A vegetative method of propagation in which a plant part, usually a shoot (graft-wood), is joined to another (rootstock) so that they grow together into a single plant. A farm operation done with use of an instrument called a harrow, which serves to break up and level soil or land. The action of gathering a crop (picking fruits during their season). Treatment given to a plant part (seed, stem, shoot) to enhance growth and development, such as a higher percentage of germination or root on cuttings. Growing two or more crops together in the same field. A ground cover system in which dry plant parts (e.g. grass) are used around the newly transplanted tree to protect soil and land against erosion and to retain soil humidity. Toxic to nerves or nervous system. A place to protect and grow plants for sale, transplanting or experimentation. A type of propagation through which small pieces of plant (explants) are propagated vegetatively in artificial culture medium in lab conditions. The male gametophyte, originating from a microspore, which fertilises the ovule to form the egg or zygote, and finally the seed. The process of breaking up and turning over the soil or land surface, or cutting furrows in preparation for sowing. Part of the mother plant (graft, bud, stem or shoot), which will be used for vegetative propagation. The process of cutting off or removing dead or living branches of a plant in order to improve shape or growth. A seedling used as the root system and lower portion of a woody plant to which the graft or bud of a more desirable plant is attached. 26

Anthesis Apomictic seed Budding Climacteric fruits Cover-cropping

Cutting

Deciduous tree Defoliation Dichogamy Etiolate Genotype Grafting

Harrowing Harvesting Hormonal treatment Inter-cropping Mulching

Neurotoxic Nursery Micro-propagation Pollen Ploughing Propagules Pruning Rootstock

Sap Scarification Scion Seedbed Seedling medium Seed propagation Fruit thinning Top dressing fertilization Transplanting Vegetative propagation Windbreak

The watery fluid that circulates through a plant, carrying food and other substances to the tissues. The process of cutting or eliminating the seed coat to enhance germination. A bud containing part from the upper portion of a plant, which is grafted onto another plant used as rootstock. Place where seed is sown. The medium (substrate) used for sowing seeds. A process through which a new plant is produced by sowing a seed. The process of eliminating deformed or small fruits to enhance growth and size of the other fruits in the panicles or branches. Application of fertiliser to the surface of the soil around a tree. The act of transferring a plant, usually a young plant, to another place, or the uprooting and replanting of a growing tree. A process of producing a new plant by vegetative means, involving the rooting, grafting, and budding of pieces of plant. A system in which a row of trees is used to break the force of the wind and avoid problems to the main crop, such as the falling of fruit.

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Why Grow Annona Trees?

These technical notes relate mainly to: Cherimoya Annona cherimola Sugar apple Annona squamosa Soursop Annona reticulata

1. Nutritional value
Fruit: Fruit pulp eaten as desert. Pulp processed into frozen juice, sherbet, ice cream.
Annona fruit

2. Socio-economic value
Leaves: Used to stuff pillows. Fruit: Fresh fruit sold in markets. Pulp mixed with wine, milk or yoghurt. Soursop: Flavouring for ice cream. Essential oils help flavouring processed fruit products. Bark: Fibres used for rope. Bark for yellow-brown dye.
Leaf

3. Medicinal value
Leaves: Contain substances with mosquito insecticide properties. Used to treat sleeping sickness. Used to control lice in hen coops. Fruit: Fruit pulp contains vitamin C. Good for teeth, bones, skin and muscle. Flower: Has been used to treat eye inflammation. Seed: Crushed seeds have insecticidal properties against fruit flies and lice. Paste of seed powder used to treat head lice. Used in cancer treatment. Bark: Extracts used to treat skin diseases and control intestinal worms. Root: Extracts used to treat cancerous tumours.

Annona twig

B E N E F I T S

Annona flower

Annona seed

Annona roots

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2006 Southampton Centre for Underutilised Crops, UK

Where To Grow Annona Trees?

F I E L D E S T A B L I S H M E N T 1b

Annonas grow in most parts of the tropics, Central and South America, the Caribbean, Africa, India, Malaysia, Philippines, Sri Lanka, and many countries of South East Asia.

1.

Climate requirements
Rainfall
mm Cherimoya range

Cherimoya: Best Average: 10001500 mm per year. 1500 Sugar apple and soursop: can survive from 300 500mm per year. Cannot survive below 300 mm. Annonas require a initiation. Temperature Cherimoya: best average in summer 1725C. In winter 518C frost will kill it. Sugar apple and soursop: 2030C. Cannot survive low temperature.

1000 Sugar apple & soursop range Cannot survive Rainfall Co 30 Sugar apple & soursop Cherimoya range range

long dry season to assist fruit 500

300 100

2. Altitude
Cherimoya: 900 to 2500 m. Sugar apple and Soursop: sea level to 1000 m.
20 10 0

3. Soils
Will grow on a wide range of soils from sandy to heavy loams, pH 6.0 7.6 . Must be good waterlogging. drainage. Cannot tolerate

Cannot survive Temperature

2006 Southampton Centre for Underutilised Crops, UK

What To Grow?
Before deciding what kind of Annona fruit should be planted, a grower should know what commercial varieties (cultivars) are available in the region, because they usually command the best prices at market.

1. Characteristics
Differences among the Annona species and cultivars determine the vegetative characteristics e.g. foliage and fruit yield, size, shape, colour, quality and number of seeds in the fruit. Because of this there exist several cultivars of each annona species. The most important commercial cultivars are the following: Cherimoya: Whaley, Pinks Mammoth and Mosman (Australia), Concha Lisa, Bronceada (Chile), Fino de Jete (Spain), White, Bays, Golden Russet, Libby and Lisa (USA), Funchal and Mateus I (Portugal), Burtons Wonder and Reretai (New Zealand), Kabri and Malalai (Israel). Soursop: Morada, Lisa, Blanca (Colombia), Giant of Alagoas, Selection of Ibimirim, Cerradina (Brazil). Sugar apple: Red Sugar Apple (USA), Noi (Thailand), Molate and Lobo (Philippines), IPA Selections (Brazil); Cuban Seedless (Cuba), Barbados and British Guinea (local selections widely propagated), Balanagar, Red Sitaphal (India).
Sugar apple

Cherimoya

Soursop

S E L E C T I O N

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2006 Southampton Centre for Underutilised Crops, UK

How To Grow Annona? - Parts of Annona Used for Propagation 1. Seeds

It is not recommended to grow seedling annona trees due to high variability.

P R O P A G A T I O N

Use seeds to grow rootstocks prior to grafting. Collect seeds when fruit is mature from strongly growing trees with desired characteristics.

2. Mature trees
Used for collecting scions (bud or grafting sticks). Good characteristics:

Big crown Good harvest of fruit with good shape, hard skin, high quality flesh.

Abundant flowers High natural fruit

No pests or disease

Strong trunk

Root system adapted to local conditions

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2006 Southampton Centre for Underutilised Crops, UK

How To Grow The Annona Tree? - Propagation by Seed Trees from seed usually produce inferior fruit.

1. Stages in seed propagation for rootstocks

Open fruit to extract seeds

S E E D P R O P A G A T I O N

Wash seeds Sow seeds within one week of collecting Air dry seeds

2. Seed treatment
Sow seeds within one week after removal from fruit for better germination. Before sowing, Soak cherimoya seeds in water for 4872 hours, or Heat seeds in water at 92C for 1015 minutes. Improve germination by up to 30% for sugar apple and soursop seeds by soaking in 1000 ppm gibberellic acid solution for 24 hours.
Soak seeds

Heat seeds

3. Seeding medium
2 parts fine river sand to 1 part topsoil.

4. Sowing seed
Sow 12 seeds in plastic bags, clay pots or tins.
Clay pot Plastic bag

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2006 Southampton Centre for Underutilised Crops, UK

How To Grow The Annona Tree? - Propagation by Seed -

S E E D P R O P A G A T I O N

1.

Seedbed

Spacing: 3 cm between plants, 10 cm between rows.

Transplant when seedlings are 1015 cm tall. Spacing: 20 cm between plants, 40 cm between rows.

Water seeds daily during dry season and every 23 days in wet season. Remove weeds weekly. Apply foliar fertiliser: 5 g urea + 15 g triple super phosphate in 1 litre water. Repeat monthly from 90 days after germination.

2. New seedling
Grow seedlings in nursery until stems are 1 cm thick. It may take 1520 months to reach this stage. They are now ready for grafting.

A young seedling

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2006 Southampton Centre for Underutilised Crops, UK

How To Grow The Annona Tree - Vegetative Propagation Vegetative propagation: The process of joining high quality plant material (the
scion) to a rooted seedling (rootstock) adapted to a specific soil and climate.

1. Advantages of vegetative propagation

Ensures the characteristics of the new tree are the same as mother tree. Trees come into fruit-bearing sooner. Cherimoya and soursop: budding and grafting is best carried out when sap begins to flow after a dry period. Sugar apple: Best time for budding is just before leaf drop during dry season. Can be up to 80% success rate.

V E G E T A T I V E P R O P A G A T I O N 4a

2. Equipment required
Secateurs to cut bud sticks Clean sharp knife Plastic tape
Secateurs Clean, sharp knife Plastic tape

3. Rootstock collection and preparation

Collect seed and sow as in Seed propagation. Seedling is ready for budding or grafting when 15 to 20 months old or when stem is just thicker than a pencil.

4. Scion collection and preparation

Cut bud stick (scion) from mature fruit bearing mother tree when leaves about to drop. Buds should be large.

2006 Southampton Centre for Underutilised Crops, UK

V E G E T A T I V E P R O P A G A T I O N 4b

1. Inverted T -budding method

(75 80% success rate) A) and B) rootstock cut and prepared. C) and D) removing bud from selected cultivar.

E) and F) insertion of the bud into the rootstock stem. G) wrapping of the bud.
.

2. Splice-grafting method
(Up to 90% success rate) A) Annona rootstock. B) Preparing rootstock. C) Joining scion to rootstock. D) Binding scion to rootstock with plastic bag protection. E) New grafted plant.

2006 Southampton Centre for Underutilised Crops, UK

How To Grow The Annona Tree - Field Establishment 1. Land preparation

If land is more than 3% slope, construct contour ridges or eyebrow terraces. If land is flat and liable to water logging construct drainage ditches. Remove shrubs and weeds before ploughing.

Contour terrace Eyebrow terrace made from stones

F I E L D E S T A B L I S H M E N T 5a

Drainage ditch Planting on flat land Use regular spacing

2. Plant spacing
Spacing varies greatly in commercial orchards. Seedling trees have wider spacing. Budded and grafted trees have closer spacing. Cherimoya 6 x 4 m to 8 x 6 m Soursop 4 x 4 m to 8 x 8 m Sugar apple 3 x 3 m to 5 x 5 m On poor rainfed wastelands can plant 4 m apart.

60cm

A
60 cm

3. Time of planting
At beginning of rains. If irrigation is available, it is possible to plant anytime. Transplant from nursery when plants are 3046 cm high or when 46 leaves have matured. (Cherimoya may be planted at 1 m tall.)

2006 Southampton Centre for Underutilised Crops, UK

F I E L D M A N A G E M E N T

How To Grow The Annona Tree - Field Management 1. Pollination

Hand pollinate to improve fruit set.

2. Annona pests
Remove affected branches or fruit

Trunk borer Fruit borer Seed borer

3. Pruning

6. Fertiliser 4. Weeding
Keep area under tree free of weeds. Mulch with grass or rice husks (beware of fire). Apply in planting hole: Farmyard manure 510 kg Lime 200300 g Phosphorus 800 g Fruit bearing trees (rate per tree): 3 kg ammonium sulphate 660 g triple super phosphate 500 g potassium chloride Apply in 3 equal amounts at beginning, middle and end of rains.

5. Irrigation
12 l water per day during establishment and dry season.

7. Intercropping
Pearl millet, beans or vegetables. Perennial legumes or grass cover crops can reduce erosion of soil and improve its physical structure.

8. Windbreaks

5b

Windbreaks such as Casuarina help protect shallow-rooted annonas.

2006 Southampton Centre for Underutilised Crops, UK

Harvesting
1. Time to first harvest
Annonas usually begin to bear fruit at between 36 years old depending on propagation method, cultural practices and climate.

2. Harvest point
Determined by skin colour Cherimoya from greyish green to yellowish green Sugar apple from greyish green to yellowish green Soursop from dark green to yellowish green

3. Skin colour index

Use the skin colour index for different markets. Local market: Pick when 20 40% yellowish skin. Will ripen 46 days later. Export market: Pick when 10-20% yellowish skin. Note: If picked at 75% yellowish will ripen in 3 days.

4. Picking fruit
Use ladder to reach high up fruit (Be careful to lean against strong branches). or Use a picking pole. Clip stalk 1 cm from fruit to reduce spoilage.

H A R V E S T I N G

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2006 Southampton Centre for Underutilised Crops, UK

P O S T H A R V E S T H A N D L I N G 6b

Post-harvest Handling And Storage

Place fruit into a basket or box with padding inside.

Place box under shade for better keeping. Store on racks in a shed. Cherimoya: 1112oC Sugar apple: 1516oC Soursop: 1620oC

Control fungus infections. Immerse in fungicide (thiobendazole 8 g/l). Dry fruit. Place in polythene packaging with ethylene absorber (clay brick wetted with potassium permanganate solution).

2006 Southampton Centre for Underutilised Crops, UK

- Preparation of Soursop PulpMost annonas are sold as fresh fruits, but they can be processed as juice, frozen pulp, jelly and ice cream. Soursop is good for processing because its pulp does not oxidise easily.

Processing

Preparation of pulp
Rinse fruit

Hand peel

Remove seeds

Heat pulp for 1 minute at 80oC Add 10g sodium benzoate per 10kg pulp Blend for 10 minutes

Sieve Add sugar Eliminate air by compression

Sterilisation of jars
Wash jars

P P R R O O C C E E S S S S II N N G G

Concentrate at 60oC

Put jars in a pan with water Boil jars for 10 minutes Remove jars

Put in storage container and

Label

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2006 Southampton Centre for Underutilised Crops, UK 2005 International Centre for Underutilised Crops, UK

Marketing and Economics

1. Local market
Annona fruits are usually sold fresh at local markets. or Transported to urban markets for sale as fresh fruits or processed into other products.

M A R K E T I N G
&

2. International market
Due to the perishable nature of annona fruits, most is sold in local and national markets. There is a growing demand for fresh cherimoya fruits in USA, Japan and Argentina. Most international trade is through processed fruit products such as juice, flavouring for ice cream and sherberts.

3. Income potential
Mean costs to establish and maintain 1 hectare soursop with 204 plants/ha.
Year Establishment/ Maintenance (US$) 2,486 716 675 923 996 1,184 1,212 Average yield (tonnes fruit/ ha) 2 4 5 7 9 10 Gross income (US$) 0 1,360 2,720 3,400 4,760 6,120 6,800 Annual Net income (US$) -2,486 644 2,045 2,477 3,764 4,936 5,588

1st 2
nd rd th

5th 6 7
th th

E C O N O M I C S 7b

2006 Southampton Centre for Underutilised Crops, UK