CLASSIFICATION TEST FOR Carmina Pea, Martin Lorenzo Pineda, Jan Marc Punongbayan, Maria Mediatrix Saraos, Nympha

Elisa Sia, Bito Solima Jr. Group 8 2C Medical Technology Organic Chemistry Laboratory ABSTRACT Different classification tests were performed in this experiment in order to . Carboxylic acids are

EXPERIMENTAL
A.SAMPLES USED/ MATERIALS

acetyl chloride, acetic anhydride, ethyl acetate, acetamide, benzamide, 2% ethanolic AgNO3, saturated NaHCO3, 25% NaOH soln., 10% NaOH soln., 20%NaOH soln., glacial acetic acid, 95% ethanol, conc. H2SO4, aniline, 1M HCl soln., alcoholic NH2OHHCl, 5%FeCl3 soln., 1M KOH, test tubes, beaker, marbles/parafilm, litmus paper
B.PROCEDURE 1. Hydrolysis of Acid Derivatives For Acyl halides and Acid Anhydride, 1 ml of water was placed in two separate test tubes. 10 drops of sample, which is acetyl chloride and acetic anhydride, were added into separate test tubes in a drop wise manner. Warming effect was noted. The resulting mixture was divided into two portions. To the 1st, 1 ml of 2% AgNO3 was added and formation of precipitate was observed. While to the 2nd portion, 1 ml of saturated NaHCO3 was added. Evolution of gas was noted.

For Esters, 2 ml of 25% NaOH solution was added to a 1 ml sample which is the ethyl acetate. The mouth of the test tube was covered with a marble or masking tape then it was heated in a boiling water bath for 5 minutes. After that, the mixture was neutralized with 10% HCl solution. With a wafting motion, the odor of the solution was noted. For Amides, 1 ml of benzamide was treated with 5 ml 10% NaOH solution then it was heated until boiling. While heating, the reaction of test evolution was done by holding a piece of moist red litmus paper over the test tube. 2. Alcoholysis: Schotten-Baumann Reaction 10 drops of acetic acid, 1 ml ethanol and 5 drops of conc. H2SO4 was over a water bath for 2 minutes. The odor of the ester formed was noted. Another test tube with 0.5 ml of ethanol, 1 ml of water, and 0.2 ml of acyl halide sample (acetyl chloride) or acid anhydride sample (acetic acid) was combined with 20% NaOH solution. The tubes were covered with a cork or parafilm and the mixture was shaken for several minutes. The

odor of the ester formed and the formation of two layers were noted. C. Aminolysis: Anilide Formation Few drops of acetyl chloride or acetic anhydride sample were added to 0.5 ml aniline. The mixture was transferred to a new test tube containing 5 ml of water. The formation of precipitate was noted. D. Hydroxamic Acid Test A preliminary test was done first before the test itself. In here, 2 drops of the sample which is ethyl acetate or acetamide was mixed with 1 ml of 95% ethanol and 1 M HCl. The color produced was noted when 1 drop of 5% FeCl3 solution was added to the mixture. When the preliminary test produced a yellow color solution, the test proper was done. Two drops of sample was added to 2 ml of alcoholic NH2OHHCl, and 1 M KOH. The mixture was heated in a boiling water bath for 2 minutes. It was cooled and 1 ml of 5% FeCl3 solution was added. A deep burgundy color was observed, indicated a positive result.