Brooke T. Kidwell 520 South Cannon Ave, Apt #1 * Hagerstown, MD 21740 * (443) 523- 5454 * bk117fc6 a@westpost.

net PROFILE: * Wrote various scientific papers for requirements of a Masters Degree at Hood C ollege: Research Proposal, Animal Study Proposal, and Mock NIH-Grant Proposal (E ffects of Age of NIH Swiss Mice on Mouse Parvovirus 1 Detection) * Developed Excel database/worksheet to improve inventory control at the AHDL. * Motivated and resilient student in managing working full-time job and pursuing a graduate degree. WORK EXPERIENCE: SAIC-Frederick, Inc. Research Technician May 2001-April 2009 Worked in a viral serological and molecular diagnostic labs using various immuno logical and molecular biological techniques to detect pathogens: * BIO-Plex Suspension Array reader from BIO-RAD to detect viruses and bacteria i n sera samples of mice using MFIA (Multiplexed Flurometric Immunoassay) * Performed ELISA (Enzyme-Linked Immuosorbent Assay) on mouse sera. * Performed HAI (Hemagglutination Inhibition Assay) as a confirmatory test of po sitive results from the ELISA and MFIA. * Performed IFA (Indirect Immunoflourscent Assay) as a confirmatory test of posi tive results from the ELISA and MFIA * Use of an automated plate reader to determine the optical density score of ELI SA samples. * DNA extraction of viral and bacterial DNA from lymph nodes/ lung, and fecal pe llets * Gel electrophoresis * Data analysis of results from viral serology and molecular diagnostic lab. SAIC-Frederick, Inc. Research Technician Sept 1999- May 2001 Worked in Clinical Monitoring Laboratory supporting clinical protocols using var ious Immunological and molecular biological techniques: * Ficoll fractionation of whole blood to separate components: plasma, sera, buff y coat, red blood cells * Use of a coulter counter to determine the viability and concentration of white blood cells * Cryopreservation of white blood cells using a cryomed * DNA extraction from buffy coat of blood of patients in the RAD-TECH study, ali quot samples for inventory * UV Spectrophotometer to read OD260 and OD280 * Data analysis and entry of results into clinical database * Performed inventory control for repository storage. BBI Biotech Research Labs, Inc. Lab technician I May 1998-Aug 1999 Worked in the Repository/Fresh Lab recording sample inventory, and processed blo od of HIV, HPV, Hepatitis, HBV and Cancer patients using various immunological t echniques: * * y * s * Aspetic Technique Ficoll fractionation of whole blood to separate components: plasma, sera, buff coat, red blood cells Hemocytometer to determine the viability and concentration of white blood cell Use of a cryomed to cyroperserve white blood cells

EDUCATION:

Hood Graduate School, Frederick, MD. Graduated: 05/22/2010 Master's of Science in Biomedical Sciences Concentration Microbiology/Immunology/ Virology Towson State University, Towson, MD Graduated: 12/1996 Bachelors of Science, Biology Concentration in Microbiology, Minor in Chemistry Additional skills and Techniques: Animal Experience: * Euthanized mice, using a kill box and a carbon dioxide source, to perform a pa rtial necropsy to remove mesenteric lymph node(s), spleen, kidney, liver, and fe cal pellet(s) from the colon of mice. * Taken the ALAT and LAT courses needed to be certified Molecular Biological: * DNA/RNA Purification * cDNA cloning and expression of cloned genes. Other: * Blood borne pathogen training * IATA training (dangerous goods shipping) * Ordering Supplies and Inventory Control * Performed maintenance on BioPlex, plate washer, pipettes, etc. * Made controls and reagents for serology testing, general reagents and master mix for PCR * Writing proper SOP's for different studies Computer Experience: * Word 2003, 2007 * Excel 2003, 2007 * Access 2003, 2007 * Power Point 2003, 2007 * Outlook 2003, 2007 * Endnote X * Adobe acrobat PDF maker * Various Bioinformatics software: Claustal X, Claustal W, TreeView, PHYLIP for Windows, Genious program, BioEdit, CLC work bench program, Primer X, etc. * Windows 98, ME, XP and VISTA * Troubleshooting computer problems