Olusegun James Oshota Flat 75B Nick Everton House 78 Grafton Street Manchester M13 9LR http://www.mcisb.org/people/index.

html Tel: +44 788-146-7970 Email: oo12fab4e@westpost.net Personal Profile Personality: Good communication skills, able to work independently, flexible and teamoriented attitude. Systems biology, metabolic network modelling and simulation skills: In silico mo delling and simulations using genome-scale yeast metabolic reconstruction, pathway and network analysis techniques (elementary flux mode analysis and flux balance anal ysis) with the aid of various softwares including Copasi, R, Matlab, Mathematica and , various bioinformatics and DNA microarray analysis tools. Other computing skills include PERL and Java programming languages, HTML, JavaScript, and PHP, Linux and MYSQL. Laboratory experience and skills: Experience in molecular biology and microbiolo gy including bacterial and yeast cell culture techniques and construction of yeast mutant strains using genetic crosses and PCR generated strategies. Other skills are DNA extraction, cell-based cloning techniques, ELISA, microscopy, proficiency in ste rile techniques, DNA microarray printing, microarray hybridization, various biochemic al, haematological (including blood group serology) and histological techniques. Education 2007 - 2011 DPhil in Systems Biology, Integrative Systems Biology Doctoral Train ing Centre, University of Manchester, UK. The objective of my PhD project is to identify potentially interesting biotechnological products through examination of the metabolic network of the organism. In this project, I apply metabolic network modelling and simulation techniques, elementary flux mode analysis (EFM) and flux balance analysis (FBA) on yeast genome-scale reconstruction models to probe the metabolic network of the organism for the possibilities of redirecting carbon fluxes towards increase d yield of a number of biotechnological products. The resulting target gene deletion strategies for increased production of an amino acid from the EFM modeling are being verified experimentally in the wet experiment Laboratory. 2006 2007 Master of Research (MRes) in Bioinformatics and Computational Biology , University of Leeds, UK pass with distinction. Course content: Bioinformatics of Protein Sequence, Structure and Function, Bioinformatics Project, Bioscience Core Skills 1 (Transferable), Bioinformatics Programming (Java, SQL, Servelets), Bioinformatics Programming (Perl), Bioinformatics of Transcriptomics and Proteomics. 1999 - 2000 MSc in Pharmacology and Biotechnology, Sheffield Hallam University, Sheffield, UK. Course content: Recombinant DNA technology, Molecular pharmacology, Molecular biotechnology, Human molecular genetics, Cell biology, Bioinformatics, Research methods and data analysis, Toxicology and New drug development. 1993 (Jan Sept) Post-graduate diploma (1st yr. MSc) in Clinical Biochemistry, Department of Chemical Pathology & Immunology, University of Leeds - Pass with distinction. Completed training in Clinical Biochemistry.

1983 1988 AIMLS Diploma in Chemical Pathology, University College Hospital, Iba dan, Nigeria. Completed training in Biomedical Sciences - Haematology, Chemical pathology, Histopathology, Microbiology and Serology (Major area - Chemical Pathology). Work Experience June, 2001 Sept, 2006 Scientist Health Protection Agency, (HPA - Porton), Porton Down, Salisbury, UK Duties involved microarray experiments and data analysis to study molecular gene tics and biochemical pathways of pathogenic mycobacteria; other microarray experience inc ludes printing of microarrays, development of DNA microarrays for the detection of pat hogens and also development of Nanochip SDA assay (genotyping and detection). Other activit ies involved development of multiplex PCR assays for the detection of pathogens, and molecula r genetics (PCR, cell-based cloning, DNA recombinants, in-vitro RNA transcription, baculovi rus expression system) of CCHF virus; performed ELISAs for the presence of FMDV (foo t and mouth disease virus); report validation; preparation of reagents for immunoassay of FMDV; data logging, checking and analysis of sample material. Dec, 1997 - Mar, 1998 Research Medical Laboratory Scientist Haematology Department, St. Michael's Hospital, Toronto, Canada Performed research experiments involving platelet and bleeding time measurements : part of a Phase III Clinical drug trial involving two cardiovascular drugs. Laboratory kil ls - platelet aggregation (Helena packs 4), bleeding time measurements, PTT measurements, data entry and its retrieval from Laboratory computers. Nov, 1995 - Oct, 1996 Research Medical Laboratory Assistant Haematology Department, Seacroft Hospital, Leeds, UK. Performed research experiments and data processing: part of a Phase III Clinical drug trial involving a cardiovascular drug. Laboratory skills - platelet aggregation (chron olog), bleeding time measurements, PTT measurements, fluorescent microscopy, data entry and its retrieval from Laboratory computers. Feb, 1995 - Oct, 1996 Part-time Medical Laboratory Scientist Clinical Laboratories Ltd, Leeds, UK. Performed biochemical and haematological analyses. Jun, 1994 - Feb, 1995 Phlebotomist Pathology Department, Harrogate General Hospital, Harrogate, UK. Performed phlebotomy, Microbiological and Biochemical analyses. Laboratory skill s - bacterial cultural methods, sensitivity tests, data entry and its retrieval from Laborator y computers. June, 1990 - Dec, 1992 Medical Laboratory Scientist II Clinical Pathology Department, Lagos University Teaching Hospital, Lagos, Nigeri a. Performed routine and emergency biochemical analyses. Supervised student Medical Laboratory Scientists and participated in analytical research work. Laboratory skills - man ual and automated biochemical techniques, including Immunoelectrophoresis and chromatography.

Apr, 1989 - Jun, 1990 Medical Laboratory Scientist I Laboratory Department, Seventh Day Adventist Hospital, Ile-Ife, Nigeria Performed general routine investigative analyses. Laboratory skills biochemistr y, microbiology, electrophoresis and blood group serology. Publication and Research 1. Dobson et al (2010). Further developments towards a genome-scale metabolic mo del of yeast. BMC Systems Biology 4:145. 2. Burton JE, Oshota OJ, North E, Hudson MJ, Polyanskaya N, Brehm J, Lloyd G, Si lman NJ (2005). Development of a multi-pathogen oligonucleotide microarray for detection of Bacillus anthracis. Mol Cell Probes, 19 (5), 349-57. 3. Burton JE, Oshota OJ, Silman NJ (2006). Differential identification of Bacill us anthracis from closely related environmental Bacillus species using microarray analysis. J Appl Microbiol 102 (4), 754-63. 4. Mutations in the tumour suppressor PTEN in endometrial cancer. (Thesis Schoo l of Science and Mathematics, Sheffield Hallam University, Sheffield (2000). 5. Agbedana E O, Taylor O and Oshota O J (1993). Problem Associated with Plasma Albumin: Estimation in Nephrotic syndrome Using Bromocresol Green Method. Afr J Med Med Sci. 1993 Sep; 22(3): 45-8. Conferences and Workshops Attended 1. Poster presentation A systems biology approach to the production of red and white biotechnological product yields using in silico systematic studies. Internationa l conference on systems biology (ICSB), Edinburg, UK - October, 2010. 2. Poster presentation A systems biology approach to the production of red and white biotechnological product yields using in silico systematic studies. Metabolic En gineering VIII: Metabolic Engineering for Green Growth (June 13-18, 2010 Jeju Island, Sout h Korea). 3. Poster presentation A systems biology approach to the production of red and white biotechnological product yields using in silico systematic studies. Inter DTC co nference at Systems Biology DTC, University of Warwick June, 2010. 4. Poster presentation Maximising red and white biotechnological product yields through in silico studies. Yeast conference, Manchester, UK July, 2009 5. Poster presentation Maximising red and white biotechnological product yields through in silico studies. Systems Biology: Integrative, Comparative, and Multi-Scale Mo deling, The 18th Annual Growth Factor and Signal Transduction Conference, Iowa state university, IOWA, USA - June 11-14, 2009 6. Oral presentation Maximising red and white biotechnological product yields t hrough in silico studies. Inter DTC conference at Systems Biology DTC, University of Manch ester 19-20 May, 2009. 7. Poster presentation Maximising red and white biotechnological product yields through in silico studies. BioSysBio conference, University of Cambridge, Cambridge, UK

23 25 March, 2009. 8. Poster presentation - FEBS-SysBio2009: The 3rd FEBS Advanced Lecture Course o n Systems Biology: From Molecular Biology to Biological Function, Alpbach, Austria , March 7-13, 2009. 9. Poster presentation - Hierarchical Regulation of Hexose Transport in Bakers Y east: A Combined Computational and Experimental Study (Interdisciplinary Research Conference, Manchester Central Convention Complex, 2-3 Oct 2008). 10. Oral presentation - Hierarchical Regulation of Hexose Transport in Bakers Ye ast: A Combined Computational and Experimental Study (Manchester Systems Biology DTC second rotation project presentation, Systems Biology DTC, University of Manches ter August, 2008. 11. Poster presentation - A preliminary study on the regulation of pyruvate deca rboxylase isozymes in Saccharomyces cerevisiae (Genomes to Systems Conference, Manchester Interdisciplinary Building, University of Manchester, Systems Biology DTC, Unive rsity of Manchester - 17-19 March 2008). Personal awards 1. Nestle Nutrition Scholarships award to study Postgraduate Diploma Course in C linical biochemistry - 1993. 2. BBSRC scholarship award to study MRes Bioinformatics course, University of Le eds, Leeds, UK 2006. 3. YTF award (The biochemical society) to attend The 3rd FEBS Advanced Lecture C ourse on Systems Biology, Austria 2009. 4. IOWA state university travel award to attend the Growth Factor and Signal Transduction Symposium on